run_metadata
92 rows where experiment.library_selection = "RANDOM" and tissue_curation_coarse = "Surface Structure"
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| Link | rowid ▼ | run.accession | experiment.accession | sample.accession | study.accession | bioproject | study.title | study.alias | study.type | study.abstract | study.attributes | study.PMIDs | sample.description | sample.title | sample.alias | sample.centername | sample.attributes | GEOsample.title | GEOsample.dataprocessing | GEOsample.source | GEOsample.treatmentprotocol | GEOsample.extractprotocol | GEOsample.growthprotocol | GEOsample.characteristics | GEOsample.accession | experiment.title | experiment.alias | experiment.library_name | experiment.design_description | experiment.library_construction_protocol | experiment.attributes | experiment.library_strategy | experiment.library_source | experiment.library_selection | experiment.library_layout | experiment.platform | experiment.instrument_model | experiment.spot_descriptor | experiment.study_ref | run.title | run.attributes | run.filename | run.semantic_name | run.total_bases | run.total_spots | run.alias | run.read_lengths | run.base_counts | run.r1_length | run.r2_length | run.r3_length | run.r4_length | run.Acount | run.Ccount | run.Gcount | run.Tcount | run.Ncount | run.experiment | run.pool_member | submission.accession | submission.srasource | submission.bioprojectsource | seqdetective.n_mates | seqdetective.mapping_rate.mate1 | seqdetective.mapping_rate.mate2 | seqdetective.nofeature_rate.mate1 | seqdetective.nofeature_rate.mate2 | seqdetective.sparsity.mate1 | seqdetective.sparsity.mate2 | seqdetective.pos_strand_rate.mate1 | seqdetective.pos_strand_rate.mate2 | seqdetective.readlen.mate1 | seqdetective.readlen.mate2 | seqdetective.judgement.mate1 | seqdetective.judgement.mate2 | seqdetective.judgement.reason | platform_family | instrument_generation | read_bias | selection_class | prep_kit | sc_or_bulk | tech_class | technology | tech_variant | submission.bioprojectsource.country | earliest_date | devstage_curation | devstage_curation_coarse | tissue_curation | tissue_curation_coarse |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 28383 | 28383 | SRR26319642 | SRX22027776 | SRS19100884 | SRP463771 | PRJNA1022139 | Danio rerio strain:AB Transcriptome or Gene expression | PRJNA1022139 | Other | Effects and Mechanisms of Porphyromonas gingivalis Outer Membrane Vesicles Induced Cardiovascular Injury | WT 3 | strain:AB|age:larvae|dev stage:48 hpi|collection date:2022 11 06|geo loc name:China:Fujian|sex:not applicable|tissue:Whole body|BioSampleModel:Model organism or animal | RNA Seq of Danio rerio:PBS treated | WT 3 | WT 3 | PBS treated | RNA-Seq | TRANSCRIPTOMIC | RANDOM | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP463771 | WT_3_1.fq.gz WT_3_2.fq.gz | fastq fastq | 6638052600.0 | 22126842.0 | WT 3 1.fq.gz | 0:150 1:150 | A:1795389898;C:1548955123;G:1524873816;T:1768758791;N:74972 | 150 | 150 | 1795389898 | 1548955123 | 1524873816 | 1768758791 | 74972 | SRX22027776 | SRS19100884 | SRA1727571 | Hospital of Stomatology, Fujian Medical University|Department of Orthodontics | Hospital of Stomatology, Fujian Medical University | 2 | 0.94512 | 0.94441 | 0.0675 | 0.06744 | 0.69457 | 0.69353 | 0.47279 | 0.46651 | 150 | 150 | B | B | biological fallback assumption | illumina | novaseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | China | 2023-10-11 | Larval | Larval | Trunk | Surface Structure | |||||||||||||||||||||
| 28384 | 28384 | SRR26316552 | SRX22024811 | SRS19097993 | SRP463771 | PRJNA1022139 | Danio rerio strain:AB Transcriptome or Gene expression | PRJNA1022139 | Other | Effects and Mechanisms of Porphyromonas gingivalis Outer Membrane Vesicles Induced Cardiovascular Injury | WT 1 | WT 1 | strain:AB|age:larvae|dev stage:48 hpi|collection date:2022 11 06|geo loc name:China:Fujian|sex:not applicable|tissue:Whole body|BioSampleModel:Model organism or animal | RNA Seq of Danio rerio:PBS treated | WT 1 | WT 1 | PBS treated | RNA-Seq | TRANSCRIPTOMIC | RANDOM | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP463771 | WT_1_1.fq.gz WT_1_2.fq.gz | fastq fastq | 6806096700.0 | 22686989.0 | WT 1 1.fq.gz | 0:150 1:150 | A:1824700900;C:1607509679;G:1583685250;T:1790098957;N:101914 | 150 | 150 | 1824700900 | 1607509679 | 1583685250 | 1790098957 | 101914 | SRX22024811 | SRS19097993 | SRA1727466 | Hospital of Stomatology, Fujian Medical University|Department of Orthodontics | Hospital of Stomatology, Fujian Medical University | 2 | 0.94441 | 0.94413 | 0.0633 | 0.06243 | 0.69589 | 0.69603 | 0.47278 | 0.46311 | 150 | 150 | B | B | biological fallback assumption | illumina | novaseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | China | 2023-10-11 | Larval | Larval | Trunk | Surface Structure | ||||||||||||||||||||
| 28385 | 28385 | SRR26298124 | SRX22006623 | SRS19081287 | SRP463771 | PRJNA1022139 | Danio rerio strain:AB Transcriptome or Gene expression | PRJNA1022139 | Other | Effects and Mechanisms of Porphyromonas gingivalis Outer Membrane Vesicles Induced Cardiovascular Injury | Pg OMVs 3 | Pg OMVs 3 | strain:AB|age:larvae|dev stage:48 hpi|collection date:2022 11 06|geo loc name:China:Fujian|sex:not applicable|tissue:whole body|BioSampleModel:Model organism or animal | RNA Seq of Danio rerio:Pg OMVs treated | Pg OMVs 3 | Pg OMVs 3 | Pg OMVs treated | RNA-Seq | TRANSCRIPTOMIC | RANDOM | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP463771 | Pgom_3_1.fq.gz Pgom_3_2.fq.gz | fastq fastq | 6799556700.0 | 22665189.0 | Pgom 3 1.fq.gz | 0:150 1:150 | A:1841955583;C:1578686846;G:1566584129;T:1812024582;N:305560 | 150 | 150 | 1841955583 | 1578686846 | 1566584129 | 1812024582 | 305560 | SRX22006623 | SRS19081287 | SRA1726653 | Hospital of Stomatology, Fujian Medical University|Department of Orthodontics | Hospital of Stomatology, Fujian Medical University | 2 | 0.93679 | 0.93643 | 0.0705 | 0.07046 | 0.69522 | 0.69572 | 0.46657 | 0.46769 | 150 | 150 | B | B | biological fallback assumption | illumina | novaseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | China | 2023-10-09 | Larval | Larval | Trunk | Surface Structure | ||||||||||||||||||||
| 28386 | 28386 | SRR26284600 | SRX21993532 | SRS19068394 | SRP463771 | PRJNA1022139 | Danio rerio strain:AB Transcriptome or Gene expression | PRJNA1022139 | Other | Effects and Mechanisms of Porphyromonas gingivalis Outer Membrane Vesicles Induced Cardiovascular Injury | Pg OMVs 2 | Pg OMVs 2 | strain:AB|age:larvae|dev stage:48 hpi|collection date:2022 11 06|geo loc name:China:Fujian|sex:not applicable|tissue:whole body|BioSampleModel:Model organism or animal | RNA Seq of Danio rerio:Pg OMVs treated | Pg OMVs 2 | Pg OMVs 2 | Pg OMVs treated | RNA-Seq | TRANSCRIPTOMIC | RANDOM | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP463771 | Pgom_2_2.fq.gz Pgom_2_1.fq.gz | fastq fastq | 6873087300.0 | 22910291.0 | Pgom 2 1.fq.gz | 0:150 1:150 | A:1852244290;C:1608380801;G:1593161224;T:1819219541;N:81444 | 150 | 150 | 1852244290 | 1608380801 | 1593161224 | 1819219541 | 81444 | SRX21993532 | SRS19068394 | SRA1726180 | Hospital of Stomatology, Fujian Medical University|Department of Orthodontics | Hospital of Stomatology, Fujian Medical University | 2 | 0.93991 | 0.93977 | 0.06917 | 0.06976 | 0.68883 | 0.68931 | 0.47575 | 0.45981 | 150 | 150 | B | B | biological fallback assumption | illumina | novaseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | China | 2023-10-06 | Larval | Larval | Trunk | Surface Structure | ||||||||||||||||||||
| 28387 | 28387 | SRR26283523 | SRX21992473 | SRS19067399 | SRP463771 | PRJNA1022139 | Danio rerio strain:AB Transcriptome or Gene expression | PRJNA1022139 | Other | Effects and Mechanisms of Porphyromonas gingivalis Outer Membrane Vesicles Induced Cardiovascular Injury | WT 2 | strain:AB|age:larvae|dev stage:48 hpi|collection date:2022 11 06|geo loc name:China:Fujian|sex:not applicable|tissue:whole body|BioSampleModel:Model organism or animal | not applicable | not applicable | not applicable | wild type | RNA-Seq | TRANSCRIPTOMIC | RANDOM | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP463771 | WT_2_1.fq.gz WT_2_2.fq.gz | fastq fastq | 6662879700.0 | 22209599.0 | WT 2 1.fq.gz | 0:150 1:150 | A:1793163738;C:1560948295;G:1543048656;T:1765563975;N:155036 | 150 | 150 | 1793163738 | 1560948295 | 1543048656 | 1765563975 | 155036 | SRX21992473 | SRS19067399 | SRA1726064 | Hospital of Stomatology, Fujian Medical University|Department of Orthodontics | Hospital of Stomatology, Fujian Medical University | 2 | 0.94479 | 0.94546 | 0.06315 | 0.06307 | 0.69751 | 0.69627 | 0.46332 | 0.45959 | 150 | 150 | B | B | biological fallback assumption | illumina | novaseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | China | 2023-10-06 | Larval | Larval | Trunk | Surface Structure | |||||||||||||||||||||
| 28388 | 28388 | SRR26213372 | SRX21923899 | SRS19008410 | SRP463771 | PRJNA1022139 | Danio rerio strain:AB Transcriptome or Gene expression | PRJNA1022139 | Other | Effects and Mechanisms of Porphyromonas gingivalis Outer Membrane Vesicles Induced Cardiovascular Injury | Model organism or animal sample from Danio rerio | Pg OMVs 1 a | strain:AB|dev stage:larvae|collection date:2022 11 06|geo loc name:China:Fujian|sex:not applicable|tissue:whole body|treatment:Pg OMVs injected|BioSampleModel:Model organism or animal | RNA seq of zebrafish:48 hpi | Pg OMVs group1a | Pg OMVs group1a | Pg OMVs injected | RNA-Seq | TRANSCRIPTOMIC | RANDOM | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP463771 | Pgom_1_1.fq.gz Pgom_1_2.fq.gz | fastq fastq | 6768097800.0 | 22560326.0 | Pgom 1 1.fq.gz | 0:150 1:150 | A:1807197563;C:1591333381;G:1591884474;T:1777600011;N:82371 | 150 | 150 | 1807197563 | 1591333381 | 1591884474 | 1777600011 | 82371 | SRX21923899 | SRS19008410 | SRA1722765 | Hospital of Stomatology, Fujian Medical University|Department of Orthodontics | Hospital of Stomatology, Fujian Medical University | 2 | 0.93975 | 0.93898 | 0.05827 | 0.05801 | 0.68521 | 0.68517 | 0.46882 | 0.46802 | 150 | 150 | B | B | biological fallback assumption | illumina | novaseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | China | 2023-09-28 | Larval | Larval | Trunk | Surface Structure | ||||||||||||||||||||
| 31473 | 31473 | SRR28362822 | SRX23967985 | SRS20767216 | SRP495638 | PRJNA1088730 | Effects of noxo1a deficiency on gene expression in zebrafish larvae upon exposure to cold warm stress | PRJNA1088730 | Other | Exposure of fish to cold warm stress was reported to elicit ROS generation and subsequent tissue damage. On the other hand ROS signaling is essential for many physiological functions of the organism. As a NADPH oxidase NOX organizer deficiency of Noxo1a is expected to decrease the content of hydrogen peroxide in fish tissue upon exposure to cold warm stress. In this study the effect on Noxo1a deficiency on gene expression in zebrafish larvae under cold warm stress was characterized by RNA sequencing. | WT re#2 | WT re#2 | strain:AB strain|dev stage:Larvae|collection date:2023 03 05|geo loc name:China: Wuhan|sex:not determined|tissue:Whole body|genotype:Wild type|treatment:Exposed to 10 °C for 12 h followed by recovery at 28 °C for 6 h|replicate:replicate = biological replicate 2|BioSampleModel:Model organism or animal | WT re#2 | WT re#2 | WT re#2 | One microgram total RNA from each sample was used as the beginning material for sequencing library construction. Each treatment included 4 biological replicates. The NEBNext rRNA Depletion Kit was used for rRNA depletion. post rRNA depletion Agencourt RNAClean XP Beads from Beckman Coulter were used for RNA purification. Then the NEBNext Ultra Directional RNA Library Prep Kit for Illumina was used for RNA fragmentation first and second strand cDNA synthesis double stranded cDNA end repair. Double strand cDNAs were purified using the Agencourt AMPure XP from Beckman Coulter and ligated to adaptors of NEBNext Multiplex Oligos for Illumina. Finally the Q5 Hot Start HiFi PCR Master Mix was used for PCR enrichment of the adaptor ligated DNA. The concentration and quality of the constructed sequencing libraries were measured by using the Agilent High Sensitivity DNA Kit and a Bioanalyzer 2100 from Agilent Technologies. The libraries were submitted to 51 bp single end high throughput sequencing by the Center for Advanced Technology CAT of the University of California San Francisco using Hiseq 3000. | RNA-Seq | TRANSCRIPTOMIC | RANDOM | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP495638 | wt-re-2_S42_L004_R1_001.fastq.gz wt-re-2_S42_L004_R2_001.fastq.gz | fastq fastq | 7360107300.0 | 24533691.0 | wt re 2 S42 L004 R1 001.fastq.gz | 0:150 1:150 | A:1953410192;C:1717566713;G:1749971750;T:1938773172;N:385473 | 150 | 150 | 1953410192 | 1717566713 | 1749971750 | 1938773172 | 385473 | SRX23967985 | SRS20767216 | SRA1825819 | Chinese Academy of Sciences|Institute of Hydrobiology | Chinese Academy of Sciences | B | B | biological fallback assumption | illumina | novaseq_era | unknown | random_priming | nebnext | bulk | unknown | unknown | China | 2024-03-16 | Larval | Larval | Trunk | Surface Structure | |||||||||||||||||||||||||||||||
| 31474 | 31474 | SRR28362829 | SRX23967984 | SRS20767215 | SRP495638 | PRJNA1088730 | Effects of noxo1a deficiency on gene expression in zebrafish larvae upon exposure to cold warm stress | PRJNA1088730 | Other | Exposure of fish to cold warm stress was reported to elicit ROS generation and subsequent tissue damage. On the other hand ROS signaling is essential for many physiological functions of the organism. As a NADPH oxidase NOX organizer deficiency of Noxo1a is expected to decrease the content of hydrogen peroxide in fish tissue upon exposure to cold warm stress. In this study the effect on Noxo1a deficiency on gene expression in zebrafish larvae under cold warm stress was characterized by RNA sequencing. | WT re#1 | WT re#1 | strain:AB strain|dev stage:Larvae|collection date:2023 03 05|geo loc name:China: Wuhan|sex:not determined|tissue:Whole body|genotype:Wild type|treatment:Exposed to 10 °C for 12 h followed by recovery at 28 °C for 6 h|replicate:replicate = biological replicate 1|BioSampleModel:Model organism or animal | WT re#1 | WT re#1 | WT re#1 | One microgram total RNA from each sample was used as the beginning material for sequencing library construction. Each treatment included 4 biological replicates. The NEBNext rRNA Depletion Kit was used for rRNA depletion. post rRNA depletion Agencourt RNAClean XP Beads from Beckman Coulter were used for RNA purification. Then the NEBNext Ultra Directional RNA Library Prep Kit for Illumina was used for RNA fragmentation first and second strand cDNA synthesis double stranded cDNA end repair. Double strand cDNAs were purified using the Agencourt AMPure XP from Beckman Coulter and ligated to adaptors of NEBNext Multiplex Oligos for Illumina. Finally the Q5 Hot Start HiFi PCR Master Mix was used for PCR enrichment of the adaptor ligated DNA. The concentration and quality of the constructed sequencing libraries were measured by using the Agilent High Sensitivity DNA Kit and a Bioanalyzer 2100 from Agilent Technologies. The libraries were submitted to 51 bp single end high throughput sequencing by the Center for Advanced Technology CAT of the University of California San Francisco using Hiseq 3000. | RNA-Seq | TRANSCRIPTOMIC | RANDOM | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP495638 | wt-re-1_S41_L004_R1_001.fastq.gz wt-re-1_S41_L004_R2_001.fastq.gz wt-re-1_S6_L002_R1_001.fastq.gz wt-re-1_S6_L002_R2_001.fastq.gz | fastq fastq fastq fastq | 7304976300.0 | 24349921.0 | wt re 1 S41 L004 R1 001.fastq.gz | 0:150 1:150 | A:1931654450;C:1711483982;G:1750280300;T:1911082544;N:475024 | 150 | 150 | 1931654450 | 1711483982 | 1750280300 | 1911082544 | 475024 | SRX23967984 | SRS20767215 | SRA1825819 | Chinese Academy of Sciences|Institute of Hydrobiology | Chinese Academy of Sciences | B | B | biological fallback assumption | illumina | novaseq_era | unknown | random_priming | nebnext | bulk | unknown | unknown | China | 2024-03-16 | Larval | Larval | Trunk | Surface Structure | |||||||||||||||||||||||||||||||
| 31475 | 31475 | SRR28362823 | SRX23967983 | SRS20767214 | SRP495638 | PRJNA1088730 | Effects of noxo1a deficiency on gene expression in zebrafish larvae upon exposure to cold warm stress | PRJNA1088730 | Other | Exposure of fish to cold warm stress was reported to elicit ROS generation and subsequent tissue damage. On the other hand ROS signaling is essential for many physiological functions of the organism. As a NADPH oxidase NOX organizer deficiency of Noxo1a is expected to decrease the content of hydrogen peroxide in fish tissue upon exposure to cold warm stress. In this study the effect on Noxo1a deficiency on gene expression in zebrafish larvae under cold warm stress was characterized by RNA sequencing. | WT cs#4 | WT cs#4 | strain:AB strain|dev stage:Larvae|collection date:2023 03 05|geo loc name:China: Wuhan|sex:not determined|tissue:Whole body|genotype:Wild type|treatment:Exposed to 10 °C for 12 h|replicate:replicate = biological replicate 4|BioSampleModel:Model organism or animal | WT cs#4 | WT cs#4 | WT cs#4 | One microgram total RNA from each sample was used as the beginning material for sequencing library construction. Each treatment included 4 biological replicates. The NEBNext rRNA Depletion Kit was used for rRNA depletion. post rRNA depletion Agencourt RNAClean XP Beads from Beckman Coulter were used for RNA purification. Then the NEBNext Ultra Directional RNA Library Prep Kit for Illumina was used for RNA fragmentation first and second strand cDNA synthesis double stranded cDNA end repair. Double strand cDNAs were purified using the Agencourt AMPure XP from Beckman Coulter and ligated to adaptors of NEBNext Multiplex Oligos for Illumina. Finally the Q5 Hot Start HiFi PCR Master Mix was used for PCR enrichment of the adaptor ligated DNA. The concentration and quality of the constructed sequencing libraries were measured by using the Agilent High Sensitivity DNA Kit and a Bioanalyzer 2100 from Agilent Technologies. The libraries were submitted to 51 bp single end high throughput sequencing by the Center for Advanced Technology CAT of the University of California San Francisco using Hiseq 3000. | RNA-Seq | TRANSCRIPTOMIC | RANDOM | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP495638 | wt-cs-4_S38_L004_R1_001.fastq.gz wt-cs-4_S38_L004_R2_001.fastq.gz | fastq fastq | 7117266300.0 | 23724221.0 | wt cs 4 S38 L004 R1 001.fastq.gz | 0:150 1:150 | A:1893835105;C:1654405982;G:1690442550;T:1878207126;N:375537 | 150 | 150 | 1893835105 | 1654405982 | 1690442550 | 1878207126 | 375537 | SRX23967983 | SRS20767214 | SRA1825819 | Chinese Academy of Sciences|Institute of Hydrobiology | Chinese Academy of Sciences | B | B | biological fallback assumption | illumina | novaseq_era | unknown | random_priming | nebnext | bulk | unknown | unknown | China | 2024-03-16 | Larval | Larval | Trunk | Surface Structure | |||||||||||||||||||||||||||||||
| 31476 | 31476 | SRR28362824 | SRX23967982 | SRS20767213 | SRP495638 | PRJNA1088730 | Effects of noxo1a deficiency on gene expression in zebrafish larvae upon exposure to cold warm stress | PRJNA1088730 | Other | Exposure of fish to cold warm stress was reported to elicit ROS generation and subsequent tissue damage. On the other hand ROS signaling is essential for many physiological functions of the organism. As a NADPH oxidase NOX organizer deficiency of Noxo1a is expected to decrease the content of hydrogen peroxide in fish tissue upon exposure to cold warm stress. In this study the effect on Noxo1a deficiency on gene expression in zebrafish larvae under cold warm stress was characterized by RNA sequencing. | WT cs#3 | WT cs#3 | strain:AB strain|dev stage:Larvae|collection date:2023 03 05|geo loc name:China: Wuhan|sex:not determined|tissue:Whole body|genotype:Wild type|treatment:Exposed to 10 °C for 12 h|replicate:replicate = biological replicate 3|BioSampleModel:Model organism or animal | WT cs#3 | WT cs#3 | WT cs#3 | One microgram total RNA from each sample was used as the beginning material for sequencing library construction. Each treatment included 4 biological replicates. The NEBNext rRNA Depletion Kit was used for rRNA depletion. post rRNA depletion Agencourt RNAClean XP Beads from Beckman Coulter were used for RNA purification. Then the NEBNext Ultra Directional RNA Library Prep Kit for Illumina was used for RNA fragmentation first and second strand cDNA synthesis double stranded cDNA end repair. Double strand cDNAs were purified using the Agencourt AMPure XP from Beckman Coulter and ligated to adaptors of NEBNext Multiplex Oligos for Illumina. Finally the Q5 Hot Start HiFi PCR Master Mix was used for PCR enrichment of the adaptor ligated DNA. The concentration and quality of the constructed sequencing libraries were measured by using the Agilent High Sensitivity DNA Kit and a Bioanalyzer 2100 from Agilent Technologies. The libraries were submitted to 51 bp single end high throughput sequencing by the Center for Advanced Technology CAT of the University of California San Francisco using Hiseq 3000. | RNA-Seq | TRANSCRIPTOMIC | RANDOM | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP495638 | wt-cs-3_S37_L004_R1_001.fastq.gz wt-cs-3_S37_L004_R2_001.fastq.gz | fastq fastq | 7766755500.0 | 25889185.0 | wt cs 3 S37 L004 R1 001.fastq.gz | 0:150 1:150 | A:2066456864;C:1808454260;G:1837135016;T:2054303347;N:406013 | 150 | 150 | 2066456864 | 1808454260 | 1837135016 | 2054303347 | 406013 | SRX23967982 | SRS20767213 | SRA1825819 | Chinese Academy of Sciences|Institute of Hydrobiology | Chinese Academy of Sciences | B | B | biological fallback assumption | illumina | novaseq_era | unknown | random_priming | nebnext | bulk | unknown | unknown | China | 2024-03-16 | Larval | Larval | Trunk | Surface Structure | |||||||||||||||||||||||||||||||
| 31477 | 31477 | SRR28362825 | SRX23967981 | SRS20767212 | SRP495638 | PRJNA1088730 | Effects of noxo1a deficiency on gene expression in zebrafish larvae upon exposure to cold warm stress | PRJNA1088730 | Other | Exposure of fish to cold warm stress was reported to elicit ROS generation and subsequent tissue damage. On the other hand ROS signaling is essential for many physiological functions of the organism. As a NADPH oxidase NOX organizer deficiency of Noxo1a is expected to decrease the content of hydrogen peroxide in fish tissue upon exposure to cold warm stress. In this study the effect on Noxo1a deficiency on gene expression in zebrafish larvae under cold warm stress was characterized by RNA sequencing. | WT cs#2 | WT cs#2 | strain:AB strain|dev stage:Larvae|collection date:2023 03 05|geo loc name:China: Wuhan|sex:not determined|tissue:Whole body|genotype:Wild type|treatment:Exposed to 10 °C for 12 h|replicate:replicate = biological replicate 2|BioSampleModel:Model organism or animal | WT cs#2 | WT cs#2 | WT cs#2 | One microgram total RNA from each sample was used as the beginning material for sequencing library construction. Each treatment included 4 biological replicates. The NEBNext rRNA Depletion Kit was used for rRNA depletion. post rRNA depletion Agencourt RNAClean XP Beads from Beckman Coulter were used for RNA purification. Then the NEBNext Ultra Directional RNA Library Prep Kit for Illumina was used for RNA fragmentation first and second strand cDNA synthesis double stranded cDNA end repair. Double strand cDNAs were purified using the Agencourt AMPure XP from Beckman Coulter and ligated to adaptors of NEBNext Multiplex Oligos for Illumina. Finally the Q5 Hot Start HiFi PCR Master Mix was used for PCR enrichment of the adaptor ligated DNA. The concentration and quality of the constructed sequencing libraries were measured by using the Agilent High Sensitivity DNA Kit and a Bioanalyzer 2100 from Agilent Technologies. The libraries were submitted to 51 bp single end high throughput sequencing by the Center for Advanced Technology CAT of the University of California San Francisco using Hiseq 3000. | RNA-Seq | TRANSCRIPTOMIC | RANDOM | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP495638 | wt-cs-2_S36_L004_R1_001.fastq.gz wt-cs-2_S36_L004_R2_001.fastq.gz | fastq fastq | 7863905100.0 | 26213017.0 | wt cs 2 S36 L004 R1 001.fastq.gz | 0:150 1:150 | A:2095810379;C:1826144395;G:1861582031;T:2079957538;N:410757 | 150 | 150 | 2095810379 | 1826144395 | 1861582031 | 2079957538 | 410757 | SRX23967981 | SRS20767212 | SRA1825819 | Chinese Academy of Sciences|Institute of Hydrobiology | Chinese Academy of Sciences | B | B | biological fallback assumption | illumina | novaseq_era | unknown | random_priming | nebnext | bulk | unknown | unknown | China | 2024-03-16 | Larval | Larval | Trunk | Surface Structure | |||||||||||||||||||||||||||||||
| 31478 | 31478 | SRR28362826 | SRX23967980 | SRS20767211 | SRP495638 | PRJNA1088730 | Effects of noxo1a deficiency on gene expression in zebrafish larvae upon exposure to cold warm stress | PRJNA1088730 | Other | Exposure of fish to cold warm stress was reported to elicit ROS generation and subsequent tissue damage. On the other hand ROS signaling is essential for many physiological functions of the organism. As a NADPH oxidase NOX organizer deficiency of Noxo1a is expected to decrease the content of hydrogen peroxide in fish tissue upon exposure to cold warm stress. In this study the effect on Noxo1a deficiency on gene expression in zebrafish larvae under cold warm stress was characterized by RNA sequencing. | WT cs#1 | WT cs#1 | strain:AB strain|dev stage:Larvae|collection date:2023 03 05|geo loc name:China: Wuhan|sex:not determined|tissue:Whole body|genotype:Wild type|treatment:Exposed to 10 °C for 12 h|replicate:replicate = biological replicate 1|BioSampleModel:Model organism or animal | WT cs#1 | WT cs#1 | WT cs#1 | One microgram total RNA from each sample was used as the beginning material for sequencing library construction. Each treatment included 4 biological replicates. The NEBNext rRNA Depletion Kit was used for rRNA depletion. post rRNA depletion Agencourt RNAClean XP Beads from Beckman Coulter were used for RNA purification. Then the NEBNext Ultra Directional RNA Library Prep Kit for Illumina was used for RNA fragmentation first and second strand cDNA synthesis double stranded cDNA end repair. Double strand cDNAs were purified using the Agencourt AMPure XP from Beckman Coulter and ligated to adaptors of NEBNext Multiplex Oligos for Illumina. Finally the Q5 Hot Start HiFi PCR Master Mix was used for PCR enrichment of the adaptor ligated DNA. The concentration and quality of the constructed sequencing libraries were measured by using the Agilent High Sensitivity DNA Kit and a Bioanalyzer 2100 from Agilent Technologies. The libraries were submitted to 51 bp single end high throughput sequencing by the Center for Advanced Technology CAT of the University of California San Francisco using Hiseq 3000. | RNA-Seq | TRANSCRIPTOMIC | RANDOM | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP495638 | wt-cs-1_S35_L004_R1_001.fastq.gz wt-cs-1_S35_L004_R2_001.fastq.gz | fastq fastq | 6904903200.0 | 23016344.0 | wt cs 1 S35 L004 R1 001.fastq.gz | 0:150 1:150 | A:1837602528;C:1605156041;G:1638534027;T:1823249554;N:361050 | 150 | 150 | 1837602528 | 1605156041 | 1638534027 | 1823249554 | 361050 | SRX23967980 | SRS20767211 | SRA1825819 | Chinese Academy of Sciences|Institute of Hydrobiology | Chinese Academy of Sciences | B | B | biological fallback assumption | illumina | novaseq_era | unknown | random_priming | nebnext | bulk | unknown | unknown | China | 2024-03-16 | Larval | Larval | Trunk | Surface Structure | |||||||||||||||||||||||||||||||
| 31479 | 31479 | SRR28362827 | SRX23967979 | SRS20767210 | SRP495638 | PRJNA1088730 | Effects of noxo1a deficiency on gene expression in zebrafish larvae upon exposure to cold warm stress | PRJNA1088730 | Other | Exposure of fish to cold warm stress was reported to elicit ROS generation and subsequent tissue damage. On the other hand ROS signaling is essential for many physiological functions of the organism. As a NADPH oxidase NOX organizer deficiency of Noxo1a is expected to decrease the content of hydrogen peroxide in fish tissue upon exposure to cold warm stress. In this study the effect on Noxo1a deficiency on gene expression in zebrafish larvae under cold warm stress was characterized by RNA sequencing. | WT ctrl#4 | WT ctrl#4 | strain:AB strain|dev stage:Larvae|collection date:2023 03 05|geo loc name:China: Wuhan|sex:not determined|tissue:Whole body|genotype:Wild type|treatment:Collected at 96 hpf|replicate:replicate = biological replicate 4|BioSampleModel:Model organism or animal | WT ctrl#4 | WT ctrl#4 | WT ctrl#4 | One microgram total RNA from each sample was used as the beginning material for sequencing library construction. Each treatment included 4 biological replicates. The NEBNext rRNA Depletion Kit was used for rRNA depletion. post rRNA depletion Agencourt RNAClean XP Beads from Beckman Coulter were used for RNA purification. Then the NEBNext Ultra Directional RNA Library Prep Kit for Illumina was used for RNA fragmentation first and second strand cDNA synthesis double stranded cDNA end repair. Double strand cDNAs were purified using the Agencourt AMPure XP from Beckman Coulter and ligated to adaptors of NEBNext Multiplex Oligos for Illumina. Finally the Q5 Hot Start HiFi PCR Master Mix was used for PCR enrichment of the adaptor ligated DNA. The concentration and quality of the constructed sequencing libraries were measured by using the Agilent High Sensitivity DNA Kit and a Bioanalyzer 2100 from Agilent Technologies. The libraries were submitted to 51 bp single end high throughput sequencing by the Center for Advanced Technology CAT of the University of California San Francisco using Hiseq 3000. | RNA-Seq | TRANSCRIPTOMIC | RANDOM | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP495638 | wt-ctrl-4_S20_L004_R1_001.fastq.gz wt-ctrl-4_S20_L004_R2_001.fastq.gz | fastq fastq | 6576597600.0 | 21921992.0 | wt ctrl 4 S20 L004 R1 001.fastq.gz | 0:150 1:150 | A:1706071185;C:1570838977;G:1609917484;T:1689461796;N:308158 | 150 | 150 | 1706071185 | 1570838977 | 1609917484 | 1689461796 | 308158 | SRX23967979 | SRS20767210 | SRA1825819 | Chinese Academy of Sciences|Institute of Hydrobiology | Chinese Academy of Sciences | B | B | biological fallback assumption | illumina | novaseq_era | unknown | random_priming | nebnext | bulk | unknown | unknown | China | 2024-03-16 | Larval | Larval | Trunk | Surface Structure | |||||||||||||||||||||||||||||||
| 31480 | 31480 | SRR28362828 | SRX23967978 | SRS20767209 | SRP495638 | PRJNA1088730 | Effects of noxo1a deficiency on gene expression in zebrafish larvae upon exposure to cold warm stress | PRJNA1088730 | Other | Exposure of fish to cold warm stress was reported to elicit ROS generation and subsequent tissue damage. On the other hand ROS signaling is essential for many physiological functions of the organism. As a NADPH oxidase NOX organizer deficiency of Noxo1a is expected to decrease the content of hydrogen peroxide in fish tissue upon exposure to cold warm stress. In this study the effect on Noxo1a deficiency on gene expression in zebrafish larvae under cold warm stress was characterized by RNA sequencing. | noxo1a re#4 | noxo1a re#4 | strain:AB strain|dev stage:Larvae|collection date:2023 03 05|geo loc name:China: Wuhan|sex:not determined|tissue:Whole body|genotype:noxo1a mutant with indel|treatment:Exposed to 10 °C for 12 h followed by recovery at 28 °C for 6 h|replicate:replicate = biological replicate 4|BioSampleModel:Model organism or animal | noxo1a re#4 | noxo1a re#4 | noxo1a re#4 | One microgram total RNA from each sample was used as the beginning material for sequencing library construction. Each treatment included 4 biological replicates. The NEBNext rRNA Depletion Kit was used for rRNA depletion. post rRNA depletion Agencourt RNAClean XP Beads from Beckman Coulter were used for RNA purification. Then the NEBNext Ultra Directional RNA Library Prep Kit for Illumina was used for RNA fragmentation first and second strand cDNA synthesis double stranded cDNA end repair. Double strand cDNAs were purified using the Agencourt AMPure XP from Beckman Coulter and ligated to adaptors of NEBNext Multiplex Oligos for Illumina. Finally the Q5 Hot Start HiFi PCR Master Mix was used for PCR enrichment of the adaptor ligated DNA. The concentration and quality of the constructed sequencing libraries were measured by using the Agilent High Sensitivity DNA Kit and a Bioanalyzer 2100 from Agilent Technologies. The libraries were submitted to 51 bp single end high throughput sequencing by the Center for Advanced Technology CAT of the University of California San Francisco using Hiseq 3000. | RNA-Seq | TRANSCRIPTOMIC | RANDOM | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP495638 | noxo1a-re-4_S0_L000_R1_000.fastq.gz noxo1a-re-4_S0_L000_R2_000.fastq.gz | fastq fastq | 6288486900.0 | 20961623.0 | noxo1a re 4 S0 L000 R1 000.fastq.gz | 0:150 1:150 | A:1666277839;C:1470224724;G:1500784713;T:1651119958;N:79666 | 150 | 150 | 1666277839 | 1470224724 | 1500784713 | 1651119958 | 79666 | SRX23967978 | SRS20767209 | SRA1825819 | Chinese Academy of Sciences|Institute of Hydrobiology | Chinese Academy of Sciences | B | B | biological fallback assumption | illumina | novaseq_era | unknown | random_priming | nebnext | bulk | unknown | unknown | China | 2024-03-16 | Larval | Larval | Trunk | Surface Structure | |||||||||||||||||||||||||||||||
| 31481 | 31481 | SRR28362830 | SRX23967977 | SRS20767208 | SRP495638 | PRJNA1088730 | Effects of noxo1a deficiency on gene expression in zebrafish larvae upon exposure to cold warm stress | PRJNA1088730 | Other | Exposure of fish to cold warm stress was reported to elicit ROS generation and subsequent tissue damage. On the other hand ROS signaling is essential for many physiological functions of the organism. As a NADPH oxidase NOX organizer deficiency of Noxo1a is expected to decrease the content of hydrogen peroxide in fish tissue upon exposure to cold warm stress. In this study the effect on Noxo1a deficiency on gene expression in zebrafish larvae under cold warm stress was characterized by RNA sequencing. | noxo1a re#3 | noxo1a re#3 | strain:AB strain|dev stage:Larvae|collection date:2023 03 05|geo loc name:China: Wuhan|sex:not determined|tissue:Whole body|genotype:noxo1a mutant with indel|treatment:Exposed to 10 °C for 12 h followed by recovery at 28 °C for 6 h|replicate:replicate = biological replicate 3|BioSampleModel:Model organism or animal | noxo1a re#3 | noxo1a re#3 | noxo1a re#3 | One microgram total RNA from each sample was used as the beginning material for sequencing library construction. Each treatment included 4 biological replicates. The NEBNext rRNA Depletion Kit was used for rRNA depletion. post rRNA depletion Agencourt RNAClean XP Beads from Beckman Coulter were used for RNA purification. Then the NEBNext Ultra Directional RNA Library Prep Kit for Illumina was used for RNA fragmentation first and second strand cDNA synthesis double stranded cDNA end repair. Double strand cDNAs were purified using the Agencourt AMPure XP from Beckman Coulter and ligated to adaptors of NEBNext Multiplex Oligos for Illumina. Finally the Q5 Hot Start HiFi PCR Master Mix was used for PCR enrichment of the adaptor ligated DNA. The concentration and quality of the constructed sequencing libraries were measured by using the Agilent High Sensitivity DNA Kit and a Bioanalyzer 2100 from Agilent Technologies. The libraries were submitted to 51 bp single end high throughput sequencing by the Center for Advanced Technology CAT of the University of California San Francisco using Hiseq 3000. | RNA-Seq | TRANSCRIPTOMIC | RANDOM | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP495638 | noxo1a-re-3_S1_L001_R1_001.fastq.gz noxo1a-re-3_S1_L001_R2_001.fastq.gz noxo1a-re-3_S2_L004_R1_001.fastq.gz noxo1a-re-3_S2_L004_R2_001.fastq.gz | fastq fastq fastq fastq | 6993336600.0 | 23311122.0 | noxo1a re 3 S1 L001 R1 001.fastq.gz | 0:150 1:150 | A:1857289544;C:1633216640;G:1660910386;T:1841856529;N:63501 | 150 | 150 | 1857289544 | 1633216640 | 1660910386 | 1841856529 | 63501 | SRX23967977 | SRS20767208 | SRA1825819 | Chinese Academy of Sciences|Institute of Hydrobiology | Chinese Academy of Sciences | B | B | biological fallback assumption | illumina | novaseq_era | unknown | random_priming | nebnext | bulk | unknown | unknown | China | 2024-03-16 | Larval | Larval | Trunk | Surface Structure | |||||||||||||||||||||||||||||||
| 31482 | 31482 | SRR28362831 | SRX23967976 | SRS20767207 | SRP495638 | PRJNA1088730 | Effects of noxo1a deficiency on gene expression in zebrafish larvae upon exposure to cold warm stress | PRJNA1088730 | Other | Exposure of fish to cold warm stress was reported to elicit ROS generation and subsequent tissue damage. On the other hand ROS signaling is essential for many physiological functions of the organism. As a NADPH oxidase NOX organizer deficiency of Noxo1a is expected to decrease the content of hydrogen peroxide in fish tissue upon exposure to cold warm stress. In this study the effect on Noxo1a deficiency on gene expression in zebrafish larvae under cold warm stress was characterized by RNA sequencing. | noxo1a re#2 | noxo1a re#2 | strain:AB strain|dev stage:Larvae|collection date:2023 03 05|geo loc name:China: Wuhan|sex:not determined|tissue:Whole body|genotype:noxo1a mutant with indel|treatment:Exposed to 10 °C for 12 h followed by recovery at 28 °C for 6 h|replicate:replicate = biological replicate 2|BioSampleModel:Model organism or animal | noxo1a re#2 | noxo1a re#2 | noxo1a re#2 | One microgram total RNA from each sample was used as the beginning material for sequencing library construction. Each treatment included 4 biological replicates. The NEBNext rRNA Depletion Kit was used for rRNA depletion. post rRNA depletion Agencourt RNAClean XP Beads from Beckman Coulter were used for RNA purification. Then the NEBNext Ultra Directional RNA Library Prep Kit for Illumina was used for RNA fragmentation first and second strand cDNA synthesis double stranded cDNA end repair. Double strand cDNAs were purified using the Agencourt AMPure XP from Beckman Coulter and ligated to adaptors of NEBNext Multiplex Oligos for Illumina. Finally the Q5 Hot Start HiFi PCR Master Mix was used for PCR enrichment of the adaptor ligated DNA. The concentration and quality of the constructed sequencing libraries were measured by using the Agilent High Sensitivity DNA Kit and a Bioanalyzer 2100 from Agilent Technologies. The libraries were submitted to 51 bp single end high throughput sequencing by the Center for Advanced Technology CAT of the University of California San Francisco using Hiseq 3000. | RNA-Seq | TRANSCRIPTOMIC | RANDOM | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP495638 | noxo1a-re-2_S1_L004_R1_001.fastq.gz noxo1a-re-2_S1_L004_R2_001.fastq.gz | fastq fastq | 6021534600.0 | 20071782.0 | noxo1a re 2 S1 L004 R1 001.fastq.gz | 0:150 1:150 | A:1597183597;C:1408878472;G:1434927510;T:1580500451;N:44570 | 150 | 150 | 1597183597 | 1408878472 | 1434927510 | 1580500451 | 44570 | SRX23967976 | SRS20767207 | SRA1825819 | Chinese Academy of Sciences|Institute of Hydrobiology | Chinese Academy of Sciences | B | B | biological fallback assumption | illumina | novaseq_era | unknown | random_priming | nebnext | bulk | unknown | unknown | China | 2024-03-16 | Larval | Larval | Trunk | Surface Structure | |||||||||||||||||||||||||||||||
| 31483 | 31483 | SRR28362832 | SRX23967975 | SRS20767206 | SRP495638 | PRJNA1088730 | Effects of noxo1a deficiency on gene expression in zebrafish larvae upon exposure to cold warm stress | PRJNA1088730 | Other | Exposure of fish to cold warm stress was reported to elicit ROS generation and subsequent tissue damage. On the other hand ROS signaling is essential for many physiological functions of the organism. As a NADPH oxidase NOX organizer deficiency of Noxo1a is expected to decrease the content of hydrogen peroxide in fish tissue upon exposure to cold warm stress. In this study the effect on Noxo1a deficiency on gene expression in zebrafish larvae under cold warm stress was characterized by RNA sequencing. | noxo1a re#1 | noxo1a re#1 | strain:AB strain|dev stage:Larvae|collection date:2023 03 05|geo loc name:China: Wuhan|sex:not determined|tissue:Whole body|genotype:noxo1a mutant with indel|treatment:Exposed to 10 °C for 12 h followed by recovery at 28 °C for 6 h|replicate:replicate = biological replicate 1|BioSampleModel:Model organism or animal | noxo1a re#1 | noxo1a re#1 | noxo1a re#1 | One microgram total RNA from each sample was used as the beginning material for sequencing library construction. Each treatment included 4 biological replicates. The NEBNext rRNA Depletion Kit was used for rRNA depletion. post rRNA depletion Agencourt RNAClean XP Beads from Beckman Coulter were used for RNA purification. Then the NEBNext Ultra Directional RNA Library Prep Kit for Illumina was used for RNA fragmentation first and second strand cDNA synthesis double stranded cDNA end repair. Double strand cDNAs were purified using the Agencourt AMPure XP from Beckman Coulter and ligated to adaptors of NEBNext Multiplex Oligos for Illumina. Finally the Q5 Hot Start HiFi PCR Master Mix was used for PCR enrichment of the adaptor ligated DNA. The concentration and quality of the constructed sequencing libraries were measured by using the Agilent High Sensitivity DNA Kit and a Bioanalyzer 2100 from Agilent Technologies. The libraries were submitted to 51 bp single end high throughput sequencing by the Center for Advanced Technology CAT of the University of California San Francisco using Hiseq 3000. | RNA-Seq | TRANSCRIPTOMIC | RANDOM | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP495638 | noxo1a-re-1_S44_L004_R1_001.fastq.gz noxo1a-re-1_S44_L004_R2_001.fastq.gz | fastq fastq | 6459165300.0 | 21530551.0 | noxo1a re 1 S44 L004 R1 001.fastq.gz | 0:150 1:150 | A:1704973328;C:1516599476;G:1546419572;T:1690833152;N:339772 | 150 | 150 | 1704973328 | 1516599476 | 1546419572 | 1690833152 | 339772 | SRX23967975 | SRS20767206 | SRA1825819 | Chinese Academy of Sciences|Institute of Hydrobiology | Chinese Academy of Sciences | B | B | biological fallback assumption | illumina | novaseq_era | unknown | random_priming | nebnext | bulk | unknown | unknown | China | 2024-03-16 | Larval | Larval | Trunk | Surface Structure | |||||||||||||||||||||||||||||||
| 31484 | 31484 | SRR28362833 | SRX23967974 | SRS20767205 | SRP495638 | PRJNA1088730 | Effects of noxo1a deficiency on gene expression in zebrafish larvae upon exposure to cold warm stress | PRJNA1088730 | Other | Exposure of fish to cold warm stress was reported to elicit ROS generation and subsequent tissue damage. On the other hand ROS signaling is essential for many physiological functions of the organism. As a NADPH oxidase NOX organizer deficiency of Noxo1a is expected to decrease the content of hydrogen peroxide in fish tissue upon exposure to cold warm stress. In this study the effect on Noxo1a deficiency on gene expression in zebrafish larvae under cold warm stress was characterized by RNA sequencing. | WT ctrl#3 | WT ctrl#3 | strain:AB strain|dev stage:Larvae|collection date:2023 03 05|geo loc name:China: Wuhan|sex:not determined|tissue:Whole body|genotype:Wild type|treatment:Collected at 96 hpf|replicate:replicate = biological replicate 3|BioSampleModel:Model organism or animal | WT ctrl#3 | WT ctrl#3 | WT ctrl#3 | One microgram total RNA from each sample was used as the beginning material for sequencing library construction. Each treatment included 4 biological replicates. The NEBNext rRNA Depletion Kit was used for rRNA depletion. post rRNA depletion Agencourt RNAClean XP Beads from Beckman Coulter were used for RNA purification. Then the NEBNext Ultra Directional RNA Library Prep Kit for Illumina was used for RNA fragmentation first and second strand cDNA synthesis double stranded cDNA end repair. Double strand cDNAs were purified using the Agencourt AMPure XP from Beckman Coulter and ligated to adaptors of NEBNext Multiplex Oligos for Illumina. Finally the Q5 Hot Start HiFi PCR Master Mix was used for PCR enrichment of the adaptor ligated DNA. The concentration and quality of the constructed sequencing libraries were measured by using the Agilent High Sensitivity DNA Kit and a Bioanalyzer 2100 from Agilent Technologies. The libraries were submitted to 51 bp single end high throughput sequencing by the Center for Advanced Technology CAT of the University of California San Francisco using Hiseq 3000. | RNA-Seq | TRANSCRIPTOMIC | RANDOM | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP495638 | wt-ctrl-3_S31_L004_R1_001.fastq.gz wt-ctrl-3_S31_L004_R2_001.fastq.gz | fastq fastq | 7847308200.0 | 26157694.0 | wt ctrl 3 S31 L004 R1 001.fastq.gz | 0:150 1:150 | A:2059319370;C:1853070672;G:1895075718;T:2039431518;N:410922 | 150 | 150 | 2059319370 | 1853070672 | 1895075718 | 2039431518 | 410922 | SRX23967974 | SRS20767205 | SRA1825819 | Chinese Academy of Sciences|Institute of Hydrobiology | Chinese Academy of Sciences | B | B | biological fallback assumption | illumina | novaseq_era | unknown | random_priming | nebnext | bulk | unknown | unknown | China | 2024-03-16 | Larval | Larval | Trunk | Surface Structure | |||||||||||||||||||||||||||||||
| 31485 | 31485 | SRR28362834 | SRX23967973 | SRS20767204 | SRP495638 | PRJNA1088730 | Effects of noxo1a deficiency on gene expression in zebrafish larvae upon exposure to cold warm stress | PRJNA1088730 | Other | Exposure of fish to cold warm stress was reported to elicit ROS generation and subsequent tissue damage. On the other hand ROS signaling is essential for many physiological functions of the organism. As a NADPH oxidase NOX organizer deficiency of Noxo1a is expected to decrease the content of hydrogen peroxide in fish tissue upon exposure to cold warm stress. In this study the effect on Noxo1a deficiency on gene expression in zebrafish larvae under cold warm stress was characterized by RNA sequencing. | noxo1a cs#4 | noxo1a cs#4 | strain:AB strain|dev stage:Larvae|collection date:2023 03 05|geo loc name:China: Wuhan|sex:not determined|tissue:Whole body|genotype:noxo1a mutant with indel|treatment:Exposed to 10 °C for 12 h|replicate:replicate = biological replicate 4|BioSampleModel:Model organism or animal | noxo1a cs#4 | noxo1a cs#4 | noxo1a cs#4 | One microgram total RNA from each sample was used as the beginning material for sequencing library construction. Each treatment included 4 biological replicates. The NEBNext rRNA Depletion Kit was used for rRNA depletion. post rRNA depletion Agencourt RNAClean XP Beads from Beckman Coulter were used for RNA purification. Then the NEBNext Ultra Directional RNA Library Prep Kit for Illumina was used for RNA fragmentation first and second strand cDNA synthesis double stranded cDNA end repair. Double strand cDNAs were purified using the Agencourt AMPure XP from Beckman Coulter and ligated to adaptors of NEBNext Multiplex Oligos for Illumina. Finally the Q5 Hot Start HiFi PCR Master Mix was used for PCR enrichment of the adaptor ligated DNA. The concentration and quality of the constructed sequencing libraries were measured by using the Agilent High Sensitivity DNA Kit and a Bioanalyzer 2100 from Agilent Technologies. The libraries were submitted to 51 bp single end high throughput sequencing by the Center for Advanced Technology CAT of the University of California San Francisco using Hiseq 3000. | RNA-Seq | TRANSCRIPTOMIC | RANDOM | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP495638 | noxo1a-cs-4_S23_L004_R1_001.fastq.gz noxo1a-cs-4_S23_L004_R2_001.fastq.gz | fastq fastq | 6310591800.0 | 21035306.0 | noxo1a cs 4 S23 L004 R1 001.fastq.gz | 0:150 1:150 | A:1649781865;C:1495677401;G:1531600458;T:1633236277;N:295799 | 150 | 150 | 1649781865 | 1495677401 | 1531600458 | 1633236277 | 295799 | SRX23967973 | SRS20767204 | SRA1825819 | Chinese Academy of Sciences|Institute of Hydrobiology | Chinese Academy of Sciences | B | B | biological fallback assumption | illumina | novaseq_era | unknown | random_priming | nebnext | bulk | unknown | unknown | China | 2024-03-16 | Larval | Larval | Trunk | Surface Structure | |||||||||||||||||||||||||||||||
| 31486 | 31486 | SRR28362835 | SRX23967972 | SRS20767203 | SRP495638 | PRJNA1088730 | Effects of noxo1a deficiency on gene expression in zebrafish larvae upon exposure to cold warm stress | PRJNA1088730 | Other | Exposure of fish to cold warm stress was reported to elicit ROS generation and subsequent tissue damage. On the other hand ROS signaling is essential for many physiological functions of the organism. As a NADPH oxidase NOX organizer deficiency of Noxo1a is expected to decrease the content of hydrogen peroxide in fish tissue upon exposure to cold warm stress. In this study the effect on Noxo1a deficiency on gene expression in zebrafish larvae under cold warm stress was characterized by RNA sequencing. | noxo1a cs#3 | noxo1a cs#3 | strain:AB strain|dev stage:Larvae|collection date:2023 03 05|geo loc name:China: Wuhan|sex:not determined|tissue:Whole body|genotype:noxo1a mutant with indel|treatment:Exposed to 10 °C for 12 h|replicate:replicate = biological replicate 3|BioSampleModel:Model organism or animal | noxo1a cs#3 | noxo1a cs#3 | noxo1a cs#3 | One microgram total RNA from each sample was used as the beginning material for sequencing library construction. Each treatment included 4 biological replicates. The NEBNext rRNA Depletion Kit was used for rRNA depletion. post rRNA depletion Agencourt RNAClean XP Beads from Beckman Coulter were used for RNA purification. Then the NEBNext Ultra Directional RNA Library Prep Kit for Illumina was used for RNA fragmentation first and second strand cDNA synthesis double stranded cDNA end repair. Double strand cDNAs were purified using the Agencourt AMPure XP from Beckman Coulter and ligated to adaptors of NEBNext Multiplex Oligos for Illumina. Finally the Q5 Hot Start HiFi PCR Master Mix was used for PCR enrichment of the adaptor ligated DNA. The concentration and quality of the constructed sequencing libraries were measured by using the Agilent High Sensitivity DNA Kit and a Bioanalyzer 2100 from Agilent Technologies. The libraries were submitted to 51 bp single end high throughput sequencing by the Center for Advanced Technology CAT of the University of California San Francisco using Hiseq 3000. | RNA-Seq | TRANSCRIPTOMIC | RANDOM | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP495638 | noxo1a-cs-3_S40_L004_R1_001.fastq.gz noxo1a-cs-3_S40_L004_R2_001.fastq.gz | fastq fastq | 6704622300.0 | 22348741.0 | noxo1a cs 3 S40 L004 R1 001.fastq.gz | 0:150 1:150 | A:1767126555;C:1576232837;G:1610607905;T:1750301837;N:353166 | 150 | 150 | 1767126555 | 1576232837 | 1610607905 | 1750301837 | 353166 | SRX23967972 | SRS20767203 | SRA1825819 | Chinese Academy of Sciences|Institute of Hydrobiology | Chinese Academy of Sciences | B | B | biological fallback assumption | illumina | novaseq_era | unknown | random_priming | nebnext | bulk | unknown | unknown | China | 2024-03-16 | Larval | Larval | Trunk | Surface Structure | |||||||||||||||||||||||||||||||
| 31487 | 31487 | SRR28362836 | SRX23967971 | SRS20767202 | SRP495638 | PRJNA1088730 | Effects of noxo1a deficiency on gene expression in zebrafish larvae upon exposure to cold warm stress | PRJNA1088730 | Other | Exposure of fish to cold warm stress was reported to elicit ROS generation and subsequent tissue damage. On the other hand ROS signaling is essential for many physiological functions of the organism. As a NADPH oxidase NOX organizer deficiency of Noxo1a is expected to decrease the content of hydrogen peroxide in fish tissue upon exposure to cold warm stress. In this study the effect on Noxo1a deficiency on gene expression in zebrafish larvae under cold warm stress was characterized by RNA sequencing. | noxo1a cs#2 | noxo1a cs#2 | strain:AB strain|dev stage:Larvae|collection date:2023 03 05|geo loc name:China: Wuhan|sex:not determined|tissue:Whole body|genotype:noxo1a mutant with indel|treatment:Exposed to 10 °C for 12 h|replicate:replicate = biological replicate 2|BioSampleModel:Model organism or animal | noxo1a cs#2 | noxo1a cs#2 | noxo1a cs#2 | One microgram total RNA from each sample was used as the beginning material for sequencing library construction. Each treatment included 4 biological replicates. The NEBNext rRNA Depletion Kit was used for rRNA depletion. post rRNA depletion Agencourt RNAClean XP Beads from Beckman Coulter were used for RNA purification. Then the NEBNext Ultra Directional RNA Library Prep Kit for Illumina was used for RNA fragmentation first and second strand cDNA synthesis double stranded cDNA end repair. Double strand cDNAs were purified using the Agencourt AMPure XP from Beckman Coulter and ligated to adaptors of NEBNext Multiplex Oligos for Illumina. Finally the Q5 Hot Start HiFi PCR Master Mix was used for PCR enrichment of the adaptor ligated DNA. The concentration and quality of the constructed sequencing libraries were measured by using the Agilent High Sensitivity DNA Kit and a Bioanalyzer 2100 from Agilent Technologies. The libraries were submitted to 51 bp single end high throughput sequencing by the Center for Advanced Technology CAT of the University of California San Francisco using Hiseq 3000. | RNA-Seq | TRANSCRIPTOMIC | RANDOM | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP495638 | noxo1a-cs-2_S22_L004_R1_001.fastq.gz noxo1a-cs-2_S22_L004_R2_001.fastq.gz | fastq fastq | 6500565900.0 | 21668553.0 | noxo1a cs 2 S22 L004 R1 001.fastq.gz | 0:150 1:150 | A:1717991417;C:1522293556;G:1558318730;T:1701666138;N:296059 | 150 | 150 | 1717991417 | 1522293556 | 1558318730 | 1701666138 | 296059 | SRX23967971 | SRS20767202 | SRA1825819 | Chinese Academy of Sciences|Institute of Hydrobiology | Chinese Academy of Sciences | B | B | biological fallback assumption | illumina | novaseq_era | unknown | random_priming | nebnext | bulk | unknown | unknown | China | 2024-03-16 | Larval | Larval | Trunk | Surface Structure | |||||||||||||||||||||||||||||||
| 31488 | 31488 | SRR28362837 | SRX23967970 | SRS20767201 | SRP495638 | PRJNA1088730 | Effects of noxo1a deficiency on gene expression in zebrafish larvae upon exposure to cold warm stress | PRJNA1088730 | Other | Exposure of fish to cold warm stress was reported to elicit ROS generation and subsequent tissue damage. On the other hand ROS signaling is essential for many physiological functions of the organism. As a NADPH oxidase NOX organizer deficiency of Noxo1a is expected to decrease the content of hydrogen peroxide in fish tissue upon exposure to cold warm stress. In this study the effect on Noxo1a deficiency on gene expression in zebrafish larvae under cold warm stress was characterized by RNA sequencing. | noxo1a cs#1 | noxo1a cs#1 | strain:AB strain|dev stage:Larvae|collection date:2023 03 05|geo loc name:China: Wuhan|sex:not determined|tissue:Whole body|genotype:noxo1a mutant with indel|treatment:Exposed to 10 °C for 12 h|replicate:replicate = biological replicate 1|BioSampleModel:Model organism or animal | noxo1a cs#1 | noxo1a cs#1 | noxo1a cs#1 | One microgram total RNA from each sample was used as the beginning material for sequencing library construction. Each treatment included 4 biological replicates. The NEBNext rRNA Depletion Kit was used for rRNA depletion. post rRNA depletion Agencourt RNAClean XP Beads from Beckman Coulter were used for RNA purification. Then the NEBNext Ultra Directional RNA Library Prep Kit for Illumina was used for RNA fragmentation first and second strand cDNA synthesis double stranded cDNA end repair. Double strand cDNAs were purified using the Agencourt AMPure XP from Beckman Coulter and ligated to adaptors of NEBNext Multiplex Oligos for Illumina. Finally the Q5 Hot Start HiFi PCR Master Mix was used for PCR enrichment of the adaptor ligated DNA. The concentration and quality of the constructed sequencing libraries were measured by using the Agilent High Sensitivity DNA Kit and a Bioanalyzer 2100 from Agilent Technologies. The libraries were submitted to 51 bp single end high throughput sequencing by the Center for Advanced Technology CAT of the University of California San Francisco using Hiseq 3000. | RNA-Seq | TRANSCRIPTOMIC | RANDOM | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP495638 | noxo1a-cs-1_S39_L004_R1_001.fastq.gz noxo1a-cs-1_S39_L004_R2_001.fastq.gz | fastq fastq | 6662253600.0 | 22207512.0 | noxo1a cs 1 S39 L004 R1 001.fastq.gz | 0:150 1:150 | A:1765032411;C:1556260041;G:1592453845;T:1748156183;N:351120 | 150 | 150 | 1765032411 | 1556260041 | 1592453845 | 1748156183 | 351120 | SRX23967970 | SRS20767201 | SRA1825819 | Chinese Academy of Sciences|Institute of Hydrobiology | Chinese Academy of Sciences | B | B | biological fallback assumption | illumina | novaseq_era | unknown | random_priming | nebnext | bulk | unknown | unknown | China | 2024-03-16 | Larval | Larval | Trunk | Surface Structure | |||||||||||||||||||||||||||||||
| 31489 | 31489 | SRR28362838 | SRX23967969 | SRS20767200 | SRP495638 | PRJNA1088730 | Effects of noxo1a deficiency on gene expression in zebrafish larvae upon exposure to cold warm stress | PRJNA1088730 | Other | Exposure of fish to cold warm stress was reported to elicit ROS generation and subsequent tissue damage. On the other hand ROS signaling is essential for many physiological functions of the organism. As a NADPH oxidase NOX organizer deficiency of Noxo1a is expected to decrease the content of hydrogen peroxide in fish tissue upon exposure to cold warm stress. In this study the effect on Noxo1a deficiency on gene expression in zebrafish larvae under cold warm stress was characterized by RNA sequencing. | noxo1a ctrl#4 | noxo1a ctrl#4 | strain:AB strain|dev stage:Larvae|collection date:2023 03 05|geo loc name:China: Wuhan|sex:not determined|tissue:Whole body|genotype:noxo1a mutant with indel|treatment:Collected at 96 hpf|replicate:replicate = biological replicate 4|BioSampleModel:Model organism or animal | noxo1a ctrl#4 | noxo1a ctrl#4 | noxo1a ctrl#4 | One microgram total RNA from each sample was used as the beginning material for sequencing library construction. Each treatment included 4 biological replicates. The NEBNext rRNA Depletion Kit was used for rRNA depletion. post rRNA depletion Agencourt RNAClean XP Beads from Beckman Coulter were used for RNA purification. Then the NEBNext Ultra Directional RNA Library Prep Kit for Illumina was used for RNA fragmentation first and second strand cDNA synthesis double stranded cDNA end repair. Double strand cDNAs were purified using the Agencourt AMPure XP from Beckman Coulter and ligated to adaptors of NEBNext Multiplex Oligos for Illumina. Finally the Q5 Hot Start HiFi PCR Master Mix was used for PCR enrichment of the adaptor ligated DNA. The concentration and quality of the constructed sequencing libraries were measured by using the Agilent High Sensitivity DNA Kit and a Bioanalyzer 2100 from Agilent Technologies. The libraries were submitted to 51 bp single end high throughput sequencing by the Center for Advanced Technology CAT of the University of California San Francisco using Hiseq 3000. | RNA-Seq | TRANSCRIPTOMIC | RANDOM | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP495638 | noxo1a-ctrl-4_S21_L004_R1_001.fastq.gz noxo1a-ctrl-4_S21_L004_R2_001.fastq.gz | fastq fastq | 6428415000.0 | 21428050.0 | noxo1a ctrl 4 S21 L004 R1 001.fastq.gz | 0:150 1:150 | A:1704089785;C:1501936031;G:1530024908;T:1692071168;N:293108 | 150 | 150 | 1704089785 | 1501936031 | 1530024908 | 1692071168 | 293108 | SRX23967969 | SRS20767200 | SRA1825819 | Chinese Academy of Sciences|Institute of Hydrobiology | Chinese Academy of Sciences | B | B | biological fallback assumption | illumina | novaseq_era | unknown | random_priming | nebnext | bulk | unknown | unknown | China | 2024-03-16 | Larval | Larval | Trunk | Surface Structure | |||||||||||||||||||||||||||||||
| 31490 | 31490 | SRR28362839 | SRX23967968 | SRS20767199 | SRP495638 | PRJNA1088730 | Effects of noxo1a deficiency on gene expression in zebrafish larvae upon exposure to cold warm stress | PRJNA1088730 | Other | Exposure of fish to cold warm stress was reported to elicit ROS generation and subsequent tissue damage. On the other hand ROS signaling is essential for many physiological functions of the organism. As a NADPH oxidase NOX organizer deficiency of Noxo1a is expected to decrease the content of hydrogen peroxide in fish tissue upon exposure to cold warm stress. In this study the effect on Noxo1a deficiency on gene expression in zebrafish larvae under cold warm stress was characterized by RNA sequencing. | noxo1a ctrl#3 | noxo1a ctrl#3 | strain:AB strain|dev stage:Larvae|collection date:2023 03 05|geo loc name:China: Wuhan|sex:not determined|tissue:Whole body|genotype:noxo1a mutant with indel|treatment:Collected at 96 hpf|replicate:replicate = biological replicate 3|BioSampleModel:Model organism or animal | noxo1a ctrl#3 | noxo1a ctrl#3 | noxo1a ctrl#3 | One microgram total RNA from each sample was used as the beginning material for sequencing library construction. Each treatment included 4 biological replicates. The NEBNext rRNA Depletion Kit was used for rRNA depletion. post rRNA depletion Agencourt RNAClean XP Beads from Beckman Coulter were used for RNA purification. Then the NEBNext Ultra Directional RNA Library Prep Kit for Illumina was used for RNA fragmentation first and second strand cDNA synthesis double stranded cDNA end repair. Double strand cDNAs were purified using the Agencourt AMPure XP from Beckman Coulter and ligated to adaptors of NEBNext Multiplex Oligos for Illumina. Finally the Q5 Hot Start HiFi PCR Master Mix was used for PCR enrichment of the adaptor ligated DNA. The concentration and quality of the constructed sequencing libraries were measured by using the Agilent High Sensitivity DNA Kit and a Bioanalyzer 2100 from Agilent Technologies. The libraries were submitted to 51 bp single end high throughput sequencing by the Center for Advanced Technology CAT of the University of California San Francisco using Hiseq 3000. | RNA-Seq | TRANSCRIPTOMIC | RANDOM | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP495638 | noxo1a-ctrl-3_S34_L004_R1_001.fastq.gz noxo1a-ctrl-3_S34_L004_R2_001.fastq.gz | fastq fastq | 6988991100.0 | 23296637.0 | noxo1a ctrl 3 S34 L004 R1 001.fastq.gz | 0:150 1:150 | A:1853131820;C:1633719581;G:1663738566;T:1838036906;N:364227 | 150 | 150 | 1853131820 | 1633719581 | 1663738566 | 1838036906 | 364227 | SRX23967968 | SRS20767199 | SRA1825819 | Chinese Academy of Sciences|Institute of Hydrobiology | Chinese Academy of Sciences | B | B | biological fallback assumption | illumina | novaseq_era | unknown | random_priming | nebnext | bulk | unknown | unknown | China | 2024-03-16 | Larval | Larval | Trunk | Surface Structure | |||||||||||||||||||||||||||||||
| 31491 | 31491 | SRR28362840 | SRX23967967 | SRS20767198 | SRP495638 | PRJNA1088730 | Effects of noxo1a deficiency on gene expression in zebrafish larvae upon exposure to cold warm stress | PRJNA1088730 | Other | Exposure of fish to cold warm stress was reported to elicit ROS generation and subsequent tissue damage. On the other hand ROS signaling is essential for many physiological functions of the organism. As a NADPH oxidase NOX organizer deficiency of Noxo1a is expected to decrease the content of hydrogen peroxide in fish tissue upon exposure to cold warm stress. In this study the effect on Noxo1a deficiency on gene expression in zebrafish larvae under cold warm stress was characterized by RNA sequencing. | noxo1a ctrl#2 | noxo1a ctrl#2 | strain:AB strain|dev stage:Larvae|collection date:2023 03 05|geo loc name:China: Wuhan|sex:not determined|tissue:Whole body|genotype:noxo1a mutant with indel|treatment:Collected at 96 hpf|replicate:replicate = biological replicate 2|BioSampleModel:Model organism or animal | noxo1a ctrl#2 | noxo1a ctrl#2 | noxo1a ctrl#2 | One microgram total RNA from each sample was used as the beginning material for sequencing library construction. Each treatment included 4 biological replicates. The NEBNext rRNA Depletion Kit was used for rRNA depletion. post rRNA depletion Agencourt RNAClean XP Beads from Beckman Coulter were used for RNA purification. Then the NEBNext Ultra Directional RNA Library Prep Kit for Illumina was used for RNA fragmentation first and second strand cDNA synthesis double stranded cDNA end repair. Double strand cDNAs were purified using the Agencourt AMPure XP from Beckman Coulter and ligated to adaptors of NEBNext Multiplex Oligos for Illumina. Finally the Q5 Hot Start HiFi PCR Master Mix was used for PCR enrichment of the adaptor ligated DNA. The concentration and quality of the constructed sequencing libraries were measured by using the Agilent High Sensitivity DNA Kit and a Bioanalyzer 2100 from Agilent Technologies. The libraries were submitted to 51 bp single end high throughput sequencing by the Center for Advanced Technology CAT of the University of California San Francisco using Hiseq 3000. | RNA-Seq | TRANSCRIPTOMIC | RANDOM | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP495638 | noxo1a-ctrl-2_S33_L004_R1_001.fastq.gz noxo1a-ctrl-2_S33_L004_R2_001.fastq.gz | fastq fastq | 6591167700.0 | 21970559.0 | noxo1a ctrl 2 S33 L004 R1 001.fastq.gz | 0:150 1:150 | A:1750729608;C:1535828938;G:1566173485;T:1738090384;N:345285 | 150 | 150 | 1750729608 | 1535828938 | 1566173485 | 1738090384 | 345285 | SRX23967967 | SRS20767198 | SRA1825819 | Chinese Academy of Sciences|Institute of Hydrobiology | Chinese Academy of Sciences | B | B | biological fallback assumption | illumina | novaseq_era | unknown | random_priming | nebnext | bulk | unknown | unknown | China | 2024-03-16 | Larval | Larval | Trunk | Surface Structure | |||||||||||||||||||||||||||||||
| 31492 | 31492 | SRR28362841 | SRX23967966 | SRS20767197 | SRP495638 | PRJNA1088730 | Effects of noxo1a deficiency on gene expression in zebrafish larvae upon exposure to cold warm stress | PRJNA1088730 | Other | Exposure of fish to cold warm stress was reported to elicit ROS generation and subsequent tissue damage. On the other hand ROS signaling is essential for many physiological functions of the organism. As a NADPH oxidase NOX organizer deficiency of Noxo1a is expected to decrease the content of hydrogen peroxide in fish tissue upon exposure to cold warm stress. In this study the effect on Noxo1a deficiency on gene expression in zebrafish larvae under cold warm stress was characterized by RNA sequencing. | noxo1a ctrl#1 | noxo1a ctrl#1 | strain:AB strain|dev stage:Larvae|collection date:2023 03 05|geo loc name:China: Wuhan|sex:not determined|tissue:Whole body|genotype:noxo1a mutant with indel|treatment:Collected at 96 hpf|replicate:replicate = biological replicate 1|BioSampleModel:Model organism or animal | noxo1a ctrl#1 | noxo1a ctrl#1 | noxo1a ctrl#1 | One microgram total RNA from each sample was used as the beginning material for sequencing library construction. Each treatment included 4 biological replicates. The NEBNext rRNA Depletion Kit was used for rRNA depletion. post rRNA depletion Agencourt RNAClean XP Beads from Beckman Coulter were used for RNA purification. Then the NEBNext Ultra Directional RNA Library Prep Kit for Illumina was used for RNA fragmentation first and second strand cDNA synthesis double stranded cDNA end repair. Double strand cDNAs were purified using the Agencourt AMPure XP from Beckman Coulter and ligated to adaptors of NEBNext Multiplex Oligos for Illumina. Finally the Q5 Hot Start HiFi PCR Master Mix was used for PCR enrichment of the adaptor ligated DNA. The concentration and quality of the constructed sequencing libraries were measured by using the Agilent High Sensitivity DNA Kit and a Bioanalyzer 2100 from Agilent Technologies. The libraries were submitted to 51 bp single end high throughput sequencing by the Center for Advanced Technology CAT of the University of California San Francisco using Hiseq 3000. | RNA-Seq | TRANSCRIPTOMIC | RANDOM | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP495638 | noxo1a-ctrl-1_S32_L004_R1_001.fastq.gz noxo1a-ctrl-1_S32_L004_R2_001.fastq.gz | fastq fastq | 7083186300.0 | 23610621.0 | noxo1a ctrl 1 S32 L004 R1 001.fastq.gz | 0:150 1:150 | A:1869161754;C:1663101510;G:1702666018;T:1847889969;N:367049 | 150 | 150 | 1869161754 | 1663101510 | 1702666018 | 1847889969 | 367049 | SRX23967966 | SRS20767197 | SRA1825819 | Chinese Academy of Sciences|Institute of Hydrobiology | Chinese Academy of Sciences | B | B | biological fallback assumption | illumina | novaseq_era | unknown | random_priming | nebnext | bulk | unknown | unknown | China | 2024-03-16 | Larval | Larval | Trunk | Surface Structure | |||||||||||||||||||||||||||||||
| 31493 | 31493 | SRR28362842 | SRX23967965 | SRS20767196 | SRP495638 | PRJNA1088730 | Effects of noxo1a deficiency on gene expression in zebrafish larvae upon exposure to cold warm stress | PRJNA1088730 | Other | Exposure of fish to cold warm stress was reported to elicit ROS generation and subsequent tissue damage. On the other hand ROS signaling is essential for many physiological functions of the organism. As a NADPH oxidase NOX organizer deficiency of Noxo1a is expected to decrease the content of hydrogen peroxide in fish tissue upon exposure to cold warm stress. In this study the effect on Noxo1a deficiency on gene expression in zebrafish larvae under cold warm stress was characterized by RNA sequencing. | WT re#4 | WT re#4 | strain:AB strain|dev stage:Larvae|collection date:2023 03 05|geo loc name:China: Wuhan|sex:not determined|tissue:Whole body|genotype:Wild type|treatment:Exposed to 10 °C for 12 h followed by recovery at 28 °C for 6 h|replicate:replicate = biological replicate 4|BioSampleModel:Model organism or animal | WT re#4 | WT re#4 | WT re#4 | One microgram total RNA from each sample was used as the beginning material for sequencing library construction. Each treatment included 4 biological replicates. The NEBNext rRNA Depletion Kit was used for rRNA depletion. post rRNA depletion Agencourt RNAClean XP Beads from Beckman Coulter were used for RNA purification. Then the NEBNext Ultra Directional RNA Library Prep Kit for Illumina was used for RNA fragmentation first and second strand cDNA synthesis double stranded cDNA end repair. Double strand cDNAs were purified using the Agencourt AMPure XP from Beckman Coulter and ligated to adaptors of NEBNext Multiplex Oligos for Illumina. Finally the Q5 Hot Start HiFi PCR Master Mix was used for PCR enrichment of the adaptor ligated DNA. The concentration and quality of the constructed sequencing libraries were measured by using the Agilent High Sensitivity DNA Kit and a Bioanalyzer 2100 from Agilent Technologies. The libraries were submitted to 51 bp single end high throughput sequencing by the Center for Advanced Technology CAT of the University of California San Francisco using Hiseq 3000. | RNA-Seq | TRANSCRIPTOMIC | RANDOM | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP495638 | wt-re-4_S24_L004_R1_001.fastq.gz wt-re-4_S24_L004_R2_001.fastq.gz | fastq fastq | 6571058100.0 | 21903527.0 | wt re 4 S24 L004 R1 001.fastq.gz | 0:150 1:150 | A:1733008779;C:1544891080;G:1572067431;T:1720784422;N:306388 | 150 | 150 | 1733008779 | 1544891080 | 1572067431 | 1720784422 | 306388 | SRX23967965 | SRS20767196 | SRA1825819 | Chinese Academy of Sciences|Institute of Hydrobiology | Chinese Academy of Sciences | B | B | biological fallback assumption | illumina | novaseq_era | unknown | random_priming | nebnext | bulk | unknown | unknown | China | 2024-03-16 | Larval | Larval | Trunk | Surface Structure | |||||||||||||||||||||||||||||||
| 31494 | 31494 | SRR28362843 | SRX23967964 | SRS20767195 | SRP495638 | PRJNA1088730 | Effects of noxo1a deficiency on gene expression in zebrafish larvae upon exposure to cold warm stress | PRJNA1088730 | Other | Exposure of fish to cold warm stress was reported to elicit ROS generation and subsequent tissue damage. On the other hand ROS signaling is essential for many physiological functions of the organism. As a NADPH oxidase NOX organizer deficiency of Noxo1a is expected to decrease the content of hydrogen peroxide in fish tissue upon exposure to cold warm stress. In this study the effect on Noxo1a deficiency on gene expression in zebrafish larvae under cold warm stress was characterized by RNA sequencing. | WT re#3 | WT re#3 | strain:AB strain|dev stage:Larvae|collection date:2023 03 05|geo loc name:China: Wuhan|sex:not determined|tissue:Whole body|genotype:Wild type|treatment:Exposed to 10 °C for 12 h followed by recovery at 28 °C for 6 h|replicate:replicate = biological replicate 3|BioSampleModel:Model organism or animal | WT re#3 | WT re#3 | WT re#3 | One microgram total RNA from each sample was used as the beginning material for sequencing library construction. Each treatment included 4 biological replicates. The NEBNext rRNA Depletion Kit was used for rRNA depletion. post rRNA depletion Agencourt RNAClean XP Beads from Beckman Coulter were used for RNA purification. Then the NEBNext Ultra Directional RNA Library Prep Kit for Illumina was used for RNA fragmentation first and second strand cDNA synthesis double stranded cDNA end repair. Double strand cDNAs were purified using the Agencourt AMPure XP from Beckman Coulter and ligated to adaptors of NEBNext Multiplex Oligos for Illumina. Finally the Q5 Hot Start HiFi PCR Master Mix was used for PCR enrichment of the adaptor ligated DNA. The concentration and quality of the constructed sequencing libraries were measured by using the Agilent High Sensitivity DNA Kit and a Bioanalyzer 2100 from Agilent Technologies. The libraries were submitted to 51 bp single end high throughput sequencing by the Center for Advanced Technology CAT of the University of California San Francisco using Hiseq 3000. | RNA-Seq | TRANSCRIPTOMIC | RANDOM | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP495638 | wt-re-3_S43_L004_R1_001.fastq.gz wt-re-3_S43_L004_R2_001.fastq.gz | fastq fastq | 7263273300.0 | 24210911.0 | wt re 3 S43 L004 R1 001.fastq.gz | 0:150 1:150 | A:1933925580;C:1690509128;G:1721840325;T:1916618378;N:379889 | 150 | 150 | 1933925580 | 1690509128 | 1721840325 | 1916618378 | 379889 | SRX23967964 | SRS20767195 | SRA1825819 | Chinese Academy of Sciences|Institute of Hydrobiology | Chinese Academy of Sciences | B | B | biological fallback assumption | illumina | novaseq_era | unknown | random_priming | nebnext | bulk | unknown | unknown | China | 2024-03-16 | Larval | Larval | Trunk | Surface Structure | |||||||||||||||||||||||||||||||
| 31495 | 31495 | SRR28362844 | SRX23967963 | SRS20767194 | SRP495638 | PRJNA1088730 | Effects of noxo1a deficiency on gene expression in zebrafish larvae upon exposure to cold warm stress | PRJNA1088730 | Other | Exposure of fish to cold warm stress was reported to elicit ROS generation and subsequent tissue damage. On the other hand ROS signaling is essential for many physiological functions of the organism. As a NADPH oxidase NOX organizer deficiency of Noxo1a is expected to decrease the content of hydrogen peroxide in fish tissue upon exposure to cold warm stress. In this study the effect on Noxo1a deficiency on gene expression in zebrafish larvae under cold warm stress was characterized by RNA sequencing. | WT ctrl#2 | WT ctrl#2 | strain:AB strain|dev stage:Larvae|collection date:2023 03 05|geo loc name:China: Wuhan|sex:not determined|tissue:Whole body|genotype:Wild type|treatment:Collected at 96 hpf|replicate:replicate = biological replicate 2|BioSampleModel:Model organism or animal | WT ctrl#2 | WT ctrl#2 | WT ctrl#2 | One microgram total RNA from each sample was used as the beginning material for sequencing library construction. Each treatment included 4 biological replicates. The NEBNext rRNA Depletion Kit was used for rRNA depletion. post rRNA depletion Agencourt RNAClean XP Beads from Beckman Coulter were used for RNA purification. Then the NEBNext Ultra Directional RNA Library Prep Kit for Illumina was used for RNA fragmentation first and second strand cDNA synthesis double stranded cDNA end repair. Double strand cDNAs were purified using the Agencourt AMPure XP from Beckman Coulter and ligated to adaptors of NEBNext Multiplex Oligos for Illumina. Finally the Q5 Hot Start HiFi PCR Master Mix was used for PCR enrichment of the adaptor ligated DNA. The concentration and quality of the constructed sequencing libraries were measured by using the Agilent High Sensitivity DNA Kit and a Bioanalyzer 2100 from Agilent Technologies. The libraries were submitted to 51 bp single end high throughput sequencing by the Center for Advanced Technology CAT of the University of California San Francisco using Hiseq 3000. | RNA-Seq | TRANSCRIPTOMIC | RANDOM | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP495638 | wt-ctrl-2_S19_L004_R1_001.fastq.gz wt-ctrl-2_S19_L004_R2_001.fastq.gz | fastq fastq | 6989097000.0 | 23296990.0 | wt ctrl 2 S19 L004 R1 001.fastq.gz | 0:150 1:150 | A:1831914872;C:1651035418;G:1692865635;T:1812968538;N:312537 | 150 | 150 | 1831914872 | 1651035418 | 1692865635 | 1812968538 | 312537 | SRX23967963 | SRS20767194 | SRA1825819 | Chinese Academy of Sciences|Institute of Hydrobiology | Chinese Academy of Sciences | B | B | biological fallback assumption | illumina | novaseq_era | unknown | random_priming | nebnext | bulk | unknown | unknown | China | 2024-03-16 | Larval | Larval | Trunk | Surface Structure | |||||||||||||||||||||||||||||||
| 31496 | 31496 | SRR28362845 | SRX23967962 | SRS20767193 | SRP495638 | PRJNA1088730 | Effects of noxo1a deficiency on gene expression in zebrafish larvae upon exposure to cold warm stress | PRJNA1088730 | Other | Exposure of fish to cold warm stress was reported to elicit ROS generation and subsequent tissue damage. On the other hand ROS signaling is essential for many physiological functions of the organism. As a NADPH oxidase NOX organizer deficiency of Noxo1a is expected to decrease the content of hydrogen peroxide in fish tissue upon exposure to cold warm stress. In this study the effect on Noxo1a deficiency on gene expression in zebrafish larvae under cold warm stress was characterized by RNA sequencing. | WT ctrl#1 | WT ctrl#1 | strain:AB strain|dev stage:Larvae|collection date:2023 03 05|geo loc name:China: Wuhan|sex:not determined|tissue:Whole body|genotype:Wild type|treatment:Collected at 96 hpf|replicate:replicate = biological replicate 1|BioSampleModel:Model organism or animal | WT ctrl#1 | WT ctrl#1 | WT ctrl#1 | One microgram total RNA from each sample was used as the beginning material for sequencing library construction. Each treatment included 4 biological replicates. The NEBNext rRNA Depletion Kit was used for rRNA depletion. post rRNA depletion Agencourt RNAClean XP Beads from Beckman Coulter were used for RNA purification. Then the NEBNext Ultra Directional RNA Library Prep Kit for Illumina was used for RNA fragmentation first and second strand cDNA synthesis double stranded cDNA end repair. Double strand cDNAs were purified using the Agencourt AMPure XP from Beckman Coulter and ligated to adaptors of NEBNext Multiplex Oligos for Illumina. Finally the Q5 Hot Start HiFi PCR Master Mix was used for PCR enrichment of the adaptor ligated DNA. The concentration and quality of the constructed sequencing libraries were measured by using the Agilent High Sensitivity DNA Kit and a Bioanalyzer 2100 from Agilent Technologies. The libraries were submitted to 51 bp single end high throughput sequencing by the Center for Advanced Technology CAT of the University of California San Francisco using Hiseq 3000. | RNA-Seq | TRANSCRIPTOMIC | RANDOM | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP495638 | wt-ctrl-1_S30_L004_R1_001.fastq.gz wt-ctrl-1_S30_L004_R2_001.fastq.gz | fastq fastq | 7158502800.0 | 23861676.0 | wt ctrl 1 S30 L004 R1 001.fastq.gz | 0:150 1:150 | A:1880251200;C:1687764492;G:1727478938;T:1862632997;N:375173 | 150 | 150 | 1880251200 | 1687764492 | 1727478938 | 1862632997 | 375173 | SRX23967962 | SRS20767193 | SRA1825819 | Chinese Academy of Sciences|Institute of Hydrobiology | Chinese Academy of Sciences | B | B | biological fallback assumption | illumina | novaseq_era | unknown | random_priming | nebnext | bulk | unknown | unknown | China | 2024-03-16 | Larval | Larval | Trunk | Surface Structure | |||||||||||||||||||||||||||||||
| 42197 | 42197 | SRR5485641 | SRX2768777 | SRS2152486 | SRP105410 | PRJNA384462 | Danio rerio Transcriptome or Gene expression | PRJNA384462 | Whole Genome Sequencing | 5 dpf larval zebrafish Danio rerio were exposed to 0 5 and 20mg/L of virgin polyethylene microparticles in dechlorinated water for up to 14 days. Fish were maintained at 26C throughout the experiment and fed at libitum. Whole RNA was extracted from 4 replicates of 35 pooled embryos from each treatment at day 2 and day 14 post exposure with RNeasy kit QIAGEN. The NEB libraries generated from these extracted RNA were then sequenced on 2 Illumina HiSeq 2500 SR 100bp lanes. | A 3.0.2 | ecotype:Growth Chamber|sex:male and female|tissue:Whole body|treatment:3.0.2|age:newborn|BioSampleModel:Model organism or animal | Danio rerio Raw sequence reads | A 3.0.2 | A 3.0.2 | Illumina HiSeq read information to generate gene expression values | WGS | TRANSCRIPTOMIC | RANDOM | SINGLE | ILLUMINA | Illumina HiSeq 2500 | SRP105410 | HI.4079.001.Index_4.A_R1.fastq.gz HI.4096.008.Index_4.A_R1.fastq.gz | fastq fastq | 2072387100.0 | 20723871.0 | HI.4079.001.Index 4.A R1.fastq.gz | 0:100 1:0 | A:539231555;C:478779687;G:466992364;T:587014266;N:369228 | 100 | 0 | 539231555 | 478779687 | 466992364 | 587014266 | 369228 | SRX2768777 | SRS2152486 | SRA557480 | Brandon University|Biology | Brandon University | 1 | 0.94094 | 0.13282 | 0.67566 | 0.46135 | 100 | B | usable mapping rate | illumina | hiseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | Canada | 2018-09-11 | Undetermined | Multi-stage | Trunk | Surface Structure | |||||||||||||||||||||||||||
| 42198 | 42198 | SRR5485640 | SRX2768776 | SRS2152485 | SRP105410 | PRJNA384462 | Danio rerio Transcriptome or Gene expression | PRJNA384462 | Whole Genome Sequencing | 5 dpf larval zebrafish Danio rerio were exposed to 0 5 and 20mg/L of virgin polyethylene microparticles in dechlorinated water for up to 14 days. Fish were maintained at 26C throughout the experiment and fed at libitum. Whole RNA was extracted from 4 replicates of 35 pooled embryos from each treatment at day 2 and day 14 post exposure with RNeasy kit QIAGEN. The NEB libraries generated from these extracted RNA were then sequenced on 2 Illumina HiSeq 2500 SR 100bp lanes. | B 3.5.2 | ecotype:Growth Chamber|sex:male and female|tissue:Whole body|treatment:3.5.2|age:newborn|BioSampleModel:Model organism or animal | Danio rerio Raw sequence reads | B 3.5.2 | B 3.5.2 | Illumina HiSeq read information to generate gene expression values | WGS | TRANSCRIPTOMIC | RANDOM | SINGLE | ILLUMINA | Illumina HiSeq 2500 | SRP105410 | HI.4079.001.Index_5.B_R1.fastq.gz HI.4096.008.Index_5.B_R1.fastq.gz | fastq fastq | 2236492200.0 | 22364922.0 | HI.4079.001.Index 5.B R1.fastq.gz | 0:100 1:0 | A:580646206;C:518191209;G:502712894;T:634542622;N:399269 | 100 | 0 | 580646206 | 518191209 | 502712894 | 634542622 | 399269 | SRX2768776 | SRS2152485 | SRA557480 | Brandon University|Biology | Brandon University | 1 | 0.94188 | 0.13073 | 0.67653 | 0.4635 | 100 | B | usable mapping rate | illumina | hiseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | Canada | 2018-09-11 | Undetermined | Multi-stage | Trunk | Surface Structure | |||||||||||||||||||||||||||
| 42199 | 42199 | SRR5485639 | SRX2768775 | SRS2152484 | SRP105410 | PRJNA384462 | Danio rerio Transcriptome or Gene expression | PRJNA384462 | Whole Genome Sequencing | 5 dpf larval zebrafish Danio rerio were exposed to 0 5 and 20mg/L of virgin polyethylene microparticles in dechlorinated water for up to 14 days. Fish were maintained at 26C throughout the experiment and fed at libitum. Whole RNA was extracted from 4 replicates of 35 pooled embryos from each treatment at day 2 and day 14 post exposure with RNeasy kit QIAGEN. The NEB libraries generated from these extracted RNA were then sequenced on 2 Illumina HiSeq 2500 SR 100bp lanes. | C 3.20.2 | ecotype:Growth Chamber|sex:male and female|tissue:Whole body|treatment:3.20.2|age:newborn|BioSampleModel:Model organism or animal | Danio rerio Raw sequence reads | C 3.20.2 | C 3.20.2 | Illumina HiSeq read information to generate gene expression values | WGS | TRANSCRIPTOMIC | RANDOM | SINGLE | ILLUMINA | Illumina HiSeq 2500 | SRP105410 | HI.4079.001.Index_12.C_R1.fastq.gz HI.4096.008.Index_12.C_R1.fastq.gz | fastq fastq | 1979628000.0 | 19796280.0 | HI.4079.001.Index 12.C R1.fastq.gz | 0:100 1:0 | A:519406228;C:453563284;G:442115002;T:564195493;N:347993 | 100 | 0 | 519406228 | 453563284 | 442115002 | 564195493 | 347993 | SRX2768775 | SRS2152484 | SRA557480 | Brandon University|Biology | Brandon University | 1 | 0.93946 | 0.14364 | 0.6759 | 0.46247 | 100 | B | usable mapping rate | illumina | hiseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | Canada | 2018-09-11 | Undetermined | Multi-stage | Trunk | Surface Structure | |||||||||||||||||||||||||||
| 42200 | 42200 | SRR5485638 | SRX2768774 | SRS2152483 | SRP105410 | PRJNA384462 | Danio rerio Transcriptome or Gene expression | PRJNA384462 | Whole Genome Sequencing | 5 dpf larval zebrafish Danio rerio were exposed to 0 5 and 20mg/L of virgin polyethylene microparticles in dechlorinated water for up to 14 days. Fish were maintained at 26C throughout the experiment and fed at libitum. Whole RNA was extracted from 4 replicates of 35 pooled embryos from each treatment at day 2 and day 14 post exposure with RNeasy kit QIAGEN. The NEB libraries generated from these extracted RNA were then sequenced on 2 Illumina HiSeq 2500 SR 100bp lanes. | D 3.0.14 | ecotype:Growth Chamber|sex:male and female|tissue:Whole body|treatment:3.0.14|age:newborn|BioSampleModel:Model organism or animal | Danio rerio Raw sequence reads | D 3.0.14 | D 3.0.14 | Illumina HiSeq read information to generate gene expression values | WGS | TRANSCRIPTOMIC | RANDOM | SINGLE | ILLUMINA | Illumina HiSeq 2500 | SRP105410 | HI.4079.001.Index_19.D_R1.fastq.gz HI.4096.008.Index_19.D_R1.fastq.gz | fastq fastq | 1856095600.0 | 18560956.0 | HI.4079.001.Index 19.D R1.fastq.gz | 0:100 1:0 | A:492424946;C:416504198;G:409645413;T:537196088;N:324955 | 100 | 0 | 492424946 | 416504198 | 409645413 | 537196088 | 324955 | SRX2768774 | SRS2152483 | SRA557480 | Brandon University|Biology | Brandon University | 1 | 0.9288 | 0.15497 | 0.69232 | 0.46842 | 100 | B | usable mapping rate | illumina | hiseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | Canada | 2018-09-11 | Undetermined | Multi-stage | Trunk | Surface Structure | |||||||||||||||||||||||||||
| 42201 | 42201 | SRR5485637 | SRX2768773 | SRS2152481 | SRP105410 | PRJNA384462 | Danio rerio Transcriptome or Gene expression | PRJNA384462 | Whole Genome Sequencing | 5 dpf larval zebrafish Danio rerio were exposed to 0 5 and 20mg/L of virgin polyethylene microparticles in dechlorinated water for up to 14 days. Fish were maintained at 26C throughout the experiment and fed at libitum. Whole RNA was extracted from 4 replicates of 35 pooled embryos from each treatment at day 2 and day 14 post exposure with RNeasy kit QIAGEN. The NEB libraries generated from these extracted RNA were then sequenced on 2 Illumina HiSeq 2500 SR 100bp lanes. | E 3.5.14 | ecotype:Growth Chamber|sex:male and female|tissue:Whole body|treatment:3.5.14|age:newborn|BioSampleModel:Model organism or animal | Danio rerio Raw sequence reads | E 3.5.14 | E 3.5.14 | Illumina HiSeq read information to generate gene expression values | WGS | TRANSCRIPTOMIC | RANDOM | SINGLE | ILLUMINA | Illumina HiSeq 2500 | SRP105410 | HI.4096.008.Index_1.E_R1.fastq.gz HI.4079.001.Index_1.E_R1.fastq.gz | fastq fastq | 2936761500.0 | 29367615.0 | HI.4079.001.Index 1.E R1.fastq.gz | 0:100 1:0 | A:762661816;C:675701333;G:661832769;T:836033713;N:531869 | 100 | 0 | 762661816 | 675701333 | 661832769 | 836033713 | 531869 | SRX2768773 | SRS2152481 | SRA557480 | Brandon University|Biology | Brandon University | 1 | 0.93957 | 0.12499 | 0.67714 | 0.4697 | 100 | B | usable mapping rate | illumina | hiseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | Canada | 2017-05-08 | Undetermined | Multi-stage | Trunk | Surface Structure | |||||||||||||||||||||||||||
| 42202 | 42202 | SRR5485636 | SRX2768772 | SRS2152480 | SRP105410 | PRJNA384462 | Danio rerio Transcriptome or Gene expression | PRJNA384462 | Whole Genome Sequencing | 5 dpf larval zebrafish Danio rerio were exposed to 0 5 and 20mg/L of virgin polyethylene microparticles in dechlorinated water for up to 14 days. Fish were maintained at 26C throughout the experiment and fed at libitum. Whole RNA was extracted from 4 replicates of 35 pooled embryos from each treatment at day 2 and day 14 post exposure with RNeasy kit QIAGEN. The NEB libraries generated from these extracted RNA were then sequenced on 2 Illumina HiSeq 2500 SR 100bp lanes. | F 3.20.14 | ecotype:Growth Chamber|sex:male and female|tissue:Whole body|treatment:3.20.14|age:newborn|BioSampleModel:Model organism or animal | Danio rerio Raw sequence reads | F 3.20.14 | F 3.20.14 | Illumina HiSeq read information to generate gene expression values | WGS | TRANSCRIPTOMIC | RANDOM | SINGLE | ILLUMINA | Illumina HiSeq 2500 | SRP105410 | HI.4079.001.Index_3.F_R1.fastq.gz HI.4096.008.Index_3.F_R1.fastq.gz | fastq fastq | 2169884500.0 | 21698845.0 | HI.4079.001.Index 3.F R1.fastq.gz | 0:100 1:0 | A:566808272;C:496620769;G:487930959;T:618144722;N:379778 | 100 | 0 | 566808272 | 496620769 | 487930959 | 618144722 | 379778 | SRX2768772 | SRS2152480 | SRA557480 | Brandon University|Biology | Brandon University | 1 | 0.93678 | 0.13131 | 0.67635 | 0.47972 | 100 | B | usable mapping rate | illumina | hiseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | Canada | 2018-09-11 | Undetermined | Multi-stage | Trunk | Surface Structure | |||||||||||||||||||||||||||
| 42203 | 42203 | SRR5485635 | SRX2768771 | SRS2152482 | SRP105410 | PRJNA384462 | Danio rerio Transcriptome or Gene expression | PRJNA384462 | Whole Genome Sequencing | 5 dpf larval zebrafish Danio rerio were exposed to 0 5 and 20mg/L of virgin polyethylene microparticles in dechlorinated water for up to 14 days. Fish were maintained at 26C throughout the experiment and fed at libitum. Whole RNA was extracted from 4 replicates of 35 pooled embryos from each treatment at day 2 and day 14 post exposure with RNeasy kit QIAGEN. The NEB libraries generated from these extracted RNA were then sequenced on 2 Illumina HiSeq 2500 SR 100bp lanes. | G 4.0.2 | ecotype:Growth Chamber|sex:male and female|tissue:Whole body|treatment:4.0.2|age:newborn|BioSampleModel:Model organism or animal | Danio rerio Raw sequence reads | G 4.0.2 | G 4.0.2 | Illumina HiSeq read information to generate gene expression values | WGS | TRANSCRIPTOMIC | RANDOM | SINGLE | ILLUMINA | Illumina HiSeq 2500 | SRP105410 | HI.4079.001.Index_9.G_R1.fastq.gz HI.4096.008.Index_9.G_R1.fastq.gz | fastq fastq | 2008051800.0 | 20080518.0 | HI.4079.001.Index 9.G R1.fastq.gz | 0:100 1:0 | A:518304309;C:466602325;G:455957803;T:566834696;N:352667 | 100 | 0 | 518304309 | 466602325 | 455957803 | 566834696 | 352667 | SRX2768771 | SRS2152482 | SRA557480 | Brandon University|Biology | Brandon University | 1 | 0.94445 | 0.12419 | 0.68201 | 0.46743 | 100 | B | usable mapping rate | illumina | hiseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | Canada | 2018-09-11 | Undetermined | Multi-stage | Trunk | Surface Structure | |||||||||||||||||||||||||||
| 42204 | 42204 | SRR5485634 | SRX2768770 | SRS2152479 | SRP105410 | PRJNA384462 | Danio rerio Transcriptome or Gene expression | PRJNA384462 | Whole Genome Sequencing | 5 dpf larval zebrafish Danio rerio were exposed to 0 5 and 20mg/L of virgin polyethylene microparticles in dechlorinated water for up to 14 days. Fish were maintained at 26C throughout the experiment and fed at libitum. Whole RNA was extracted from 4 replicates of 35 pooled embryos from each treatment at day 2 and day 14 post exposure with RNeasy kit QIAGEN. The NEB libraries generated from these extracted RNA were then sequenced on 2 Illumina HiSeq 2500 SR 100bp lanes. | H 4.5.2 | ecotype:Growth Chamber|sex:male and female|tissue:Whole body|treatment:4.5.2|age:newborn|BioSampleModel:Model organism or animal | Danio rerio Raw sequence reads | H 4.5.2 | H 4.5.2 | Illumina HiSeq read information to generate gene expression values | WGS | TRANSCRIPTOMIC | RANDOM | SINGLE | ILLUMINA | Illumina HiSeq 2500 | SRP105410 | HI.4079.001.Index_8.H_R1.fastq.gz HI.4096.008.Index_8.H_R1.fastq.gz | fastq fastq | 1908097900.0 | 19080979.0 | HI.4079.001.Index 8.H R1.fastq.gz | 0:100 1:0 | A:494318032;C:441931870;G:431477592;T:540030651;N:339755 | 100 | 0 | 494318032 | 441931870 | 431477592 | 540030651 | 339755 | SRX2768770 | SRS2152479 | SRA557480 | Brandon University|Biology | Brandon University | 1 | 0.94216 | 0.1306 | 0.68195 | 0.45834 | 100 | B | usable mapping rate | illumina | hiseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | Canada | 2018-09-11 | Undetermined | Multi-stage | Trunk | Surface Structure | |||||||||||||||||||||||||||
| 42205 | 42205 | SRR5485633 | SRX2768769 | SRS2152477 | SRP105410 | PRJNA384462 | Danio rerio Transcriptome or Gene expression | PRJNA384462 | Whole Genome Sequencing | 5 dpf larval zebrafish Danio rerio were exposed to 0 5 and 20mg/L of virgin polyethylene microparticles in dechlorinated water for up to 14 days. Fish were maintained at 26C throughout the experiment and fed at libitum. Whole RNA was extracted from 4 replicates of 35 pooled embryos from each treatment at day 2 and day 14 post exposure with RNeasy kit QIAGEN. The NEB libraries generated from these extracted RNA were then sequenced on 2 Illumina HiSeq 2500 SR 100bp lanes. | I 4.20.2 | ecotype:Growth Chamber|sex:male and female|tissue:Whole body|treatment:4.20.2|age:newborn|BioSampleModel:Model organism or animal | Danio rerio Raw sequence reads | I 4.20.2 | I 4.20.2 | Illumina HiSeq read information to generate gene expression values | WGS | TRANSCRIPTOMIC | RANDOM | SINGLE | ILLUMINA | Illumina HiSeq 2500 | SRP105410 | HI.4079.001.Index_10.I_R1.fastq.gz HI.4096.008.Index_10.I_R1.fastq.gz | fastq fastq | 2059931100.0 | 20599311.0 | HI.4079.001.Index 10.I R1.fastq.gz | 0:100 1:0 | A:536731862;C:475769707;G:462890515;T:584183211;N:355805 | 100 | 0 | 536731862 | 475769707 | 462890515 | 584183211 | 355805 | SRX2768769 | SRS2152477 | SRA557480 | Brandon University|Biology | Brandon University | 1 | 0.93561 | 0.13393 | 0.68225 | 0.46489 | 100 | B | usable mapping rate | illumina | hiseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | Canada | 2018-09-11 | Undetermined | Multi-stage | Trunk | Surface Structure | |||||||||||||||||||||||||||
| 42206 | 42206 | SRR5485632 | SRX2768768 | SRS2152478 | SRP105410 | PRJNA384462 | Danio rerio Transcriptome or Gene expression | PRJNA384462 | Whole Genome Sequencing | 5 dpf larval zebrafish Danio rerio were exposed to 0 5 and 20mg/L of virgin polyethylene microparticles in dechlorinated water for up to 14 days. Fish were maintained at 26C throughout the experiment and fed at libitum. Whole RNA was extracted from 4 replicates of 35 pooled embryos from each treatment at day 2 and day 14 post exposure with RNeasy kit QIAGEN. The NEB libraries generated from these extracted RNA were then sequenced on 2 Illumina HiSeq 2500 SR 100bp lanes. | J 4.0.14 | ecotype:Growth Chamber|sex:male and female|tissue:Whole body|treatment:4.0.14|age:newborn|BioSampleModel:Model organism or animal | Danio rerio Raw sequence reads | J 4.0.14 | J 4.0.14 | Illumina HiSeq read information to generate gene expression values | WGS | TRANSCRIPTOMIC | RANDOM | SINGLE | ILLUMINA | Illumina HiSeq 2500 | SRP105410 | HI.4079.001.Index_11.J_R1.fastq.gz HI.4096.008.Index_11.J_R1.fastq.gz | fastq fastq | 2199504800.0 | 21995048.0 | HI.4079.001.Index 11.J R1.fastq.gz | 0:100 1:0 | A:579625731;C:499663817;G:489173373;T:630657278;N:384601 | 100 | 0 | 579625731 | 499663817 | 489173373 | 630657278 | 384601 | SRX2768768 | SRS2152478 | SRA557480 | Brandon University|Biology | Brandon University | 1 | 0.93523 | 0.13836 | 0.68655 | 0.47839 | 100 | B | usable mapping rate | illumina | hiseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | Canada | 2018-09-11 | Undetermined | Multi-stage | Trunk | Surface Structure | |||||||||||||||||||||||||||
| 42207 | 42207 | SRR5485631 | SRX2768767 | SRS2152475 | SRP105410 | PRJNA384462 | Danio rerio Transcriptome or Gene expression | PRJNA384462 | Whole Genome Sequencing | 5 dpf larval zebrafish Danio rerio were exposed to 0 5 and 20mg/L of virgin polyethylene microparticles in dechlorinated water for up to 14 days. Fish were maintained at 26C throughout the experiment and fed at libitum. Whole RNA was extracted from 4 replicates of 35 pooled embryos from each treatment at day 2 and day 14 post exposure with RNeasy kit QIAGEN. The NEB libraries generated from these extracted RNA were then sequenced on 2 Illumina HiSeq 2500 SR 100bp lanes. | K 4.5.14 | ecotype:Growth Chamber|sex:male and female|tissue:Whole body|treatment:4.5.14|age:newborn|BioSampleModel:Model organism or animal | Danio rerio Raw sequence reads | K 4.5.14 | K 4.5.14 | Illumina HiSeq read information to generate gene expression values | WGS | TRANSCRIPTOMIC | RANDOM | SINGLE | ILLUMINA | Illumina HiSeq 2500 | SRP105410 | HI.4079.001.Index_20.K_R1.fastq.gz HI.4096.008.Index_20.K_R1.fastq.gz | fastq fastq | 2154282200.0 | 21542822.0 | HI.4079.001.Index 20.K R1.fastq.gz | 0:100 1:0 | A:563358732;C:494058898;G:482596638;T:613890813;N:377119 | 100 | 0 | 563358732 | 494058898 | 482596638 | 613890813 | 377119 | SRX2768767 | SRS2152475 | SRA557480 | Brandon University|Biology | Brandon University | 1 | 0.93863 | 0.12932 | 0.67801 | 0.46426 | 100 | B | usable mapping rate | illumina | hiseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | Canada | 2018-09-11 | Undetermined | Multi-stage | Trunk | Surface Structure | |||||||||||||||||||||||||||
| 42208 | 42208 | SRR5485630 | SRX2768766 | SRS2152476 | SRP105410 | PRJNA384462 | Danio rerio Transcriptome or Gene expression | PRJNA384462 | Whole Genome Sequencing | 5 dpf larval zebrafish Danio rerio were exposed to 0 5 and 20mg/L of virgin polyethylene microparticles in dechlorinated water for up to 14 days. Fish were maintained at 26C throughout the experiment and fed at libitum. Whole RNA was extracted from 4 replicates of 35 pooled embryos from each treatment at day 2 and day 14 post exposure with RNeasy kit QIAGEN. The NEB libraries generated from these extracted RNA were then sequenced on 2 Illumina HiSeq 2500 SR 100bp lanes. | L 4.20.14 | ecotype:Growth Chamber|sex:male and female|tissue:Whole body|treatment:4.20.14|age:newborn|BioSampleModel:Model organism or animal | Danio rerio Raw sequence reads | L 4.20.14 | L 4.20.14 | Illumina HiSeq read information to generate gene expression values | WGS | TRANSCRIPTOMIC | RANDOM | SINGLE | ILLUMINA | Illumina HiSeq 2500 | SRP105410 | HI.4096.008.Index_22.L_R1.fastq.gz HI.4079.001.Index_22.L_R1.fastq.gz | fastq fastq | 2702515700.0 | 27025157.0 | HI.4079.001.Index 22.L R1.fastq.gz | 0:100 1:0 | A:708045960;C:615042366;G:603133757;T:775807969;N:485648 | 100 | 0 | 708045960 | 615042366 | 603133757 | 775807969 | 485648 | SRX2768766 | SRS2152476 | SRA557480 | Brandon University|Biology | Brandon University | 1 | 0.93316 | 0.14421 | 0.68915 | 0.47027 | 100 | B | usable mapping rate | illumina | hiseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | Canada | 2017-05-08 | Undetermined | Multi-stage | Trunk | Surface Structure | |||||||||||||||||||||||||||
| 42209 | 42209 | SRR5485629 | SRX2768765 | SRS2152474 | SRP105410 | PRJNA384462 | Danio rerio Transcriptome or Gene expression | PRJNA384462 | Whole Genome Sequencing | 5 dpf larval zebrafish Danio rerio were exposed to 0 5 and 20mg/L of virgin polyethylene microparticles in dechlorinated water for up to 14 days. Fish were maintained at 26C throughout the experiment and fed at libitum. Whole RNA was extracted from 4 replicates of 35 pooled embryos from each treatment at day 2 and day 14 post exposure with RNeasy kit QIAGEN. The NEB libraries generated from these extracted RNA were then sequenced on 2 Illumina HiSeq 2500 SR 100bp lanes. | M 5.0.2 | ecotype:Growth Chamber|sex:male and female|tissue:Whole body|treatment:5.0.2|age:newborn|BioSampleModel:Model organism or animal | Danio rerio Raw sequence reads | M 5.0.2 | M 5.0.2 | Illumina HiSeq read information to generate gene expression values | WGS | TRANSCRIPTOMIC | RANDOM | SINGLE | ILLUMINA | Illumina HiSeq 2500 | SRP105410 | HI.4079.001.Index_25.M_R1.fastq.gz HI.4096.008.Index_25.M_R1.fastq.gz | fastq fastq | 1999183400.0 | 19991834.0 | HI.4079.001.Index 25.M R1.fastq.gz | 0:100 1:0 | A:526344986;C:456726468;G:447467581;T:568292150;N:352215 | 100 | 0 | 526344986 | 456726468 | 447467581 | 568292150 | 352215 | SRX2768765 | SRS2152474 | SRA557480 | Brandon University|Biology | Brandon University | 1 | 0.9374 | 0.14185 | 0.67665 | 0.47249 | 100 | B | usable mapping rate | illumina | hiseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | Canada | 2018-09-11 | Undetermined | Multi-stage | Trunk | Surface Structure | |||||||||||||||||||||||||||
| 42210 | 42210 | SRR5485628 | SRX2768764 | SRS2152473 | SRP105410 | PRJNA384462 | Danio rerio Transcriptome or Gene expression | PRJNA384462 | Whole Genome Sequencing | 5 dpf larval zebrafish Danio rerio were exposed to 0 5 and 20mg/L of virgin polyethylene microparticles in dechlorinated water for up to 14 days. Fish were maintained at 26C throughout the experiment and fed at libitum. Whole RNA was extracted from 4 replicates of 35 pooled embryos from each treatment at day 2 and day 14 post exposure with RNeasy kit QIAGEN. The NEB libraries generated from these extracted RNA were then sequenced on 2 Illumina HiSeq 2500 SR 100bp lanes. | N 5.5.2 | ecotype:Growth Chamber|sex:male and female|tissue:Whole body|treatment:5.5.2|age:newborn|BioSampleModel:Model organism or animal | Danio rerio Raw sequence reads | N 5.5.2 | N 5.5.2 | Illumina HiSeq read information to generate gene expression values | WGS | TRANSCRIPTOMIC | RANDOM | SINGLE | ILLUMINA | Illumina HiSeq 2500 | SRP105410 | HI.4079.001.Index_21.N_R1.fastq.gz HI.4096.008.Index_21.N_R1.fastq.gz | fastq fastq | 2097386100.0 | 20973861.0 | HI.4079.001.Index 21.N R1.fastq.gz | 0:100 1:0 | A:551586521;C:479609380;G:467572413;T:598246436;N:371350 | 100 | 0 | 551586521 | 479609380 | 467572413 | 598246436 | 371350 | SRX2768764 | SRS2152473 | SRA557480 | Brandon University|Biology | Brandon University | 1 | 0.93984 | 0.13949 | 0.68219 | 0.46921 | 100 | B | usable mapping rate | illumina | hiseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | Canada | 2018-09-11 | Undetermined | Multi-stage | Trunk | Surface Structure | |||||||||||||||||||||||||||
| 42211 | 42211 | SRR5485627 | SRX2768763 | SRS2152472 | SRP105410 | PRJNA384462 | Danio rerio Transcriptome or Gene expression | PRJNA384462 | Whole Genome Sequencing | 5 dpf larval zebrafish Danio rerio were exposed to 0 5 and 20mg/L of virgin polyethylene microparticles in dechlorinated water for up to 14 days. Fish were maintained at 26C throughout the experiment and fed at libitum. Whole RNA was extracted from 4 replicates of 35 pooled embryos from each treatment at day 2 and day 14 post exposure with RNeasy kit QIAGEN. The NEB libraries generated from these extracted RNA were then sequenced on 2 Illumina HiSeq 2500 SR 100bp lanes. | O 5.20.2 | ecotype:Growth Chamber|sex:male and female|tissue:Whole body|treatment:5.20.2|age:newborn|BioSampleModel:Model organism or animal | Danio rerio Raw sequence reads | O 5.20.2 | O 5.20.2 | Illumina HiSeq read information to generate gene expression values | WGS | TRANSCRIPTOMIC | RANDOM | SINGLE | ILLUMINA | Illumina HiSeq 2500 | SRP105410 | HI.4079.001.Index_23.O_R1.fastq.gz HI.4096.008.Index_23.O_R1.fastq.gz | fastq fastq | 2003835800.0 | 20038358.0 | HI.4079.001.Index 23.O R1.fastq.gz | 0:100 1:0 | A:529075084;C:456810826;G:444441300;T:573155538;N:353052 | 100 | 0 | 529075084 | 456810826 | 444441300 | 573155538 | 353052 | SRX2768763 | SRS2152472 | SRA557480 | Brandon University|Biology | Brandon University | 1 | 0.93635 | 0.15287 | 0.67712 | 0.45807 | 100 | B | usable mapping rate | illumina | hiseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | Canada | 2018-09-11 | Undetermined | Multi-stage | Trunk | Surface Structure | |||||||||||||||||||||||||||
| 42212 | 42212 | SRR5485626 | SRX2768762 | SRS2152471 | SRP105410 | PRJNA384462 | Danio rerio Transcriptome or Gene expression | PRJNA384462 | Whole Genome Sequencing | 5 dpf larval zebrafish Danio rerio were exposed to 0 5 and 20mg/L of virgin polyethylene microparticles in dechlorinated water for up to 14 days. Fish were maintained at 26C throughout the experiment and fed at libitum. Whole RNA was extracted from 4 replicates of 35 pooled embryos from each treatment at day 2 and day 14 post exposure with RNeasy kit QIAGEN. The NEB libraries generated from these extracted RNA were then sequenced on 2 Illumina HiSeq 2500 SR 100bp lanes. | P 5.0.14 | ecotype:Growth Chamber|sex:male and female|tissue:Whole body|treatment:5.0.14|age:newborn|BioSampleModel:Model organism or animal | Danio rerio Raw sequence reads | P 5.0.14 | P 5.0.14 | Illumina HiSeq read information to generate gene expression values | WGS | TRANSCRIPTOMIC | RANDOM | SINGLE | ILLUMINA | Illumina HiSeq 2500 | SRP105410 | HI.4079.001.Index_27.P_R1.fastq.gz HI.4096.008.Index_27.P_R1.fastq.gz | fastq fastq | 1799355500.0 | 17993555.0 | HI.4079.001.Index 27.P R1.fastq.gz | 0:100 1:0 | A:485156399;C:399414298;G:389730888;T:524733869;N:320046 | 100 | 0 | 485156399 | 399414298 | 389730888 | 524733869 | 320046 | SRX2768762 | SRS2152471 | SRA557480 | Brandon University|Biology | Brandon University | 1 | 0.92813 | 0.15749 | 0.67943 | 0.47424 | 100 | B | usable mapping rate | illumina | hiseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | Canada | 2018-09-11 | Undetermined | Multi-stage | Trunk | Surface Structure | |||||||||||||||||||||||||||
| 42213 | 42213 | SRR5485625 | SRX2768761 | SRS2152470 | SRP105410 | PRJNA384462 | Danio rerio Transcriptome or Gene expression | PRJNA384462 | Whole Genome Sequencing | 5 dpf larval zebrafish Danio rerio were exposed to 0 5 and 20mg/L of virgin polyethylene microparticles in dechlorinated water for up to 14 days. Fish were maintained at 26C throughout the experiment and fed at libitum. Whole RNA was extracted from 4 replicates of 35 pooled embryos from each treatment at day 2 and day 14 post exposure with RNeasy kit QIAGEN. The NEB libraries generated from these extracted RNA were then sequenced on 2 Illumina HiSeq 2500 SR 100bp lanes. | Q 5.5.14 | ecotype:Growth Chamber|sex:male and female|tissue:Whole body|treatment:5.5.14|age:newborn|BioSampleModel:Model organism or animal | Danio rerio Raw sequence reads | Q 5.5.14 | Q 5.5.14 | Illumina HiSeq read information to generate gene expression values | WGS | TRANSCRIPTOMIC | RANDOM | SINGLE | ILLUMINA | Illumina HiSeq 2500 | SRP105410 | HI.4079.001.Index_2.Q_R1.fastq.gz HI.4096.008.Index_2.Q_R1.fastq.gz | fastq fastq | 2260494100.0 | 22604941.0 | HI.4079.001.Index 2.Q R1.fastq.gz | 0:100 1:0 | A:602519260;C:506296484;G:495685951;T:655587703;N:404702 | 100 | 0 | 602519260 | 506296484 | 495685951 | 655587703 | 404702 | SRX2768761 | SRS2152470 | SRA557480 | Brandon University|Biology | Brandon University | 1 | 0.92524 | 0.14979 | 0.6859 | 0.47957 | 100 | B | usable mapping rate | illumina | hiseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | Canada | 2018-09-11 | Undetermined | Multi-stage | Trunk | Surface Structure | |||||||||||||||||||||||||||
| 42214 | 42214 | SRR5485624 | SRX2768760 | SRS2152469 | SRP105410 | PRJNA384462 | Danio rerio Transcriptome or Gene expression | PRJNA384462 | Whole Genome Sequencing | 5 dpf larval zebrafish Danio rerio were exposed to 0 5 and 20mg/L of virgin polyethylene microparticles in dechlorinated water for up to 14 days. Fish were maintained at 26C throughout the experiment and fed at libitum. Whole RNA was extracted from 4 replicates of 35 pooled embryos from each treatment at day 2 and day 14 post exposure with RNeasy kit QIAGEN. The NEB libraries generated from these extracted RNA were then sequenced on 2 Illumina HiSeq 2500 SR 100bp lanes. | R 5.20.14 | ecotype:Growth Chamber|sex:male and female|tissue:Whole body|treatment:5.20.14|age:newborn|BioSampleModel:Model organism or animal | Danio rerio Raw sequence reads | R 5.20.14 | R 5.20.14 | Illumina HiSeq read information to generate gene expression values | WGS | TRANSCRIPTOMIC | RANDOM | SINGLE | ILLUMINA | Illumina HiSeq 2500 | SRP105410 | HI.4079.001.Index_13.R_R1.fastq.gz HI.4096.008.Index_13.R_R1.fastq.gz | fastq fastq | 1975297900.0 | 19752979.0 | HI.4079.001.Index 13.R R1.fastq.gz | 0:100 1:0 | A:527732232;C:440160233;G:430962427;T:576091914;N:351094 | 100 | 0 | 527732232 | 440160233 | 430962427 | 576091914 | 351094 | SRX2768760 | SRS2152469 | SRA557480 | Brandon University|Biology | Brandon University | 1 | 0.92785 | 0.15864 | 0.6814 | 0.47773 | 100 | B | usable mapping rate | illumina | hiseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | Canada | 2018-09-11 | Undetermined | Multi-stage | Trunk | Surface Structure | |||||||||||||||||||||||||||
| 42215 | 42215 | SRR5485623 | SRX2768759 | SRS2152468 | SRP105410 | PRJNA384462 | Danio rerio Transcriptome or Gene expression | PRJNA384462 | Whole Genome Sequencing | 5 dpf larval zebrafish Danio rerio were exposed to 0 5 and 20mg/L of virgin polyethylene microparticles in dechlorinated water for up to 14 days. Fish were maintained at 26C throughout the experiment and fed at libitum. Whole RNA was extracted from 4 replicates of 35 pooled embryos from each treatment at day 2 and day 14 post exposure with RNeasy kit QIAGEN. The NEB libraries generated from these extracted RNA were then sequenced on 2 Illumina HiSeq 2500 SR 100bp lanes. | S 6.0.2 | ecotype:Growth Chamber|sex:male and female|tissue:Whole body|treatment:S 6.0.2|age:newborn|BioSampleModel:Model organism or animal | Danio rerio Raw sequence reads | S 6.0.2 | S 6.0.2 | Illumina HiSeq read information to generate gene expression values | WGS | TRANSCRIPTOMIC | RANDOM | SINGLE | ILLUMINA | Illumina HiSeq 2500 | SRP105410 | HI.4079.001.Index_6.S_R1.fastq.gz HI.4096.008.Index_6.S_R1.fastq.gz | fastq fastq | 2690628500.0 | 26906285.0 | HI.4079.001.Index 6.S R1.fastq.gz | 0:100 1:0 | A:713021693;C:609779787;G:594591832;T:772745072;N:490116 | 100 | 0 | 713021693 | 609779787 | 594591832 | 772745072 | 490116 | SRX2768759 | SRS2152468 | SRA557480 | Brandon University|Biology | Brandon University | 1 | 0.93405 | 0.15472 | 0.68505 | 0.45796 | 100 | B | usable mapping rate | illumina | hiseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | Canada | 2018-09-11 | Undetermined | Multi-stage | Trunk | Surface Structure | |||||||||||||||||||||||||||
| 42216 | 42216 | SRR5485622 | SRX2768758 | SRS2152466 | SRP105410 | PRJNA384462 | Danio rerio Transcriptome or Gene expression | PRJNA384462 | Whole Genome Sequencing | 5 dpf larval zebrafish Danio rerio were exposed to 0 5 and 20mg/L of virgin polyethylene microparticles in dechlorinated water for up to 14 days. Fish were maintained at 26C throughout the experiment and fed at libitum. Whole RNA was extracted from 4 replicates of 35 pooled embryos from each treatment at day 2 and day 14 post exposure with RNeasy kit QIAGEN. The NEB libraries generated from these extracted RNA were then sequenced on 2 Illumina HiSeq 2500 SR 100bp lanes. | T 6.5.2 | ecotype:Growth Chamber|sex:male and female|tissue:Whole body|treatment:6.5.2|age:newborn|BioSampleModel:Model organism or animal | Danio rerio Raw sequence reads | T 6.5.2 | T 6.5.2 | Illumina HiSeq read information to generate gene expression values | WGS | TRANSCRIPTOMIC | RANDOM | SINGLE | ILLUMINA | Illumina HiSeq 2500 | SRP105410 | HI.4079.001.Index_15.T_R1.fastq.gz HI.4096.008.Index_15.T_R1.fastq.gz | fastq fastq | 1949043500.0 | 19490435.0 | HI.4079.001.Index 15.T R1.fastq.gz | 0:100 1:0 | A:515794596;C:441198348;G:430323928;T:561379111;N:347517 | 100 | 0 | 515794596 | 441198348 | 430323928 | 561379111 | 347517 | SRX2768758 | SRS2152466 | SRA557480 | Brandon University|Biology | Brandon University | 1 | 0.93579 | 0.16207 | 0.68473 | 0.44993 | 100 | B | usable mapping rate | illumina | hiseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | Canada | 2018-09-11 | Undetermined | Multi-stage | Trunk | Surface Structure | |||||||||||||||||||||||||||
| 42217 | 42217 | SRR5485621 | SRX2768757 | SRS2152467 | SRP105410 | PRJNA384462 | Danio rerio Transcriptome or Gene expression | PRJNA384462 | Whole Genome Sequencing | 5 dpf larval zebrafish Danio rerio were exposed to 0 5 and 20mg/L of virgin polyethylene microparticles in dechlorinated water for up to 14 days. Fish were maintained at 26C throughout the experiment and fed at libitum. Whole RNA was extracted from 4 replicates of 35 pooled embryos from each treatment at day 2 and day 14 post exposure with RNeasy kit QIAGEN. The NEB libraries generated from these extracted RNA were then sequenced on 2 Illumina HiSeq 2500 SR 100bp lanes. | U 6.20.2 | ecotype:Growth Chamber|sex:male and female|tissue:Whole body|treatment:6.20.2|age:newborn|BioSampleModel:Model organism or animal | Danio rerio Raw sequence reads | U 6.20.2 | U 6.20.2 | Illumina HiSeq read information to generate gene expression values | WGS | TRANSCRIPTOMIC | RANDOM | SINGLE | ILLUMINA | Illumina HiSeq 2500 | SRP105410 | HI.4079.001.Index_7.U_R1.fastq.gz HI.4096.008.Index_7.U_R1.fastq.gz | fastq fastq | 1754851900.0 | 17548519.0 | HI.4079.001.Index 7.U R1.fastq.gz | 0:100 1:0 | A:464191451;C:397313933;G:387262108;T:505777499;N:306909 | 100 | 0 | 464191451 | 397313933 | 387262108 | 505777499 | 306909 | SRX2768757 | SRS2152467 | SRA557480 | Brandon University|Biology | Brandon University | 1 | 0.93016 | 0.16163 | 0.68329 | 0.47088 | 100 | B | usable mapping rate | illumina | hiseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | Canada | 2018-09-11 | Undetermined | Multi-stage | Trunk | Surface Structure | |||||||||||||||||||||||||||
| 42218 | 42218 | SRR5485620 | SRX2768756 | SRS2152463 | SRP105410 | PRJNA384462 | Danio rerio Transcriptome or Gene expression | PRJNA384462 | Whole Genome Sequencing | 5 dpf larval zebrafish Danio rerio were exposed to 0 5 and 20mg/L of virgin polyethylene microparticles in dechlorinated water for up to 14 days. Fish were maintained at 26C throughout the experiment and fed at libitum. Whole RNA was extracted from 4 replicates of 35 pooled embryos from each treatment at day 2 and day 14 post exposure with RNeasy kit QIAGEN. The NEB libraries generated from these extracted RNA were then sequenced on 2 Illumina HiSeq 2500 SR 100bp lanes. | V 6.0.14 | ecotype:Growth Chamber|sex:male and female|tissue:Whole body|treatment:6.0.14|age:newborn|BioSampleModel:Model organism or animal | Danio rerio Raw sequence reads | V 6.0.14 | V 6.0.14 | Illumina HiSeq read information to generate gene expression values | WGS | TRANSCRIPTOMIC | RANDOM | SINGLE | ILLUMINA | Illumina HiSeq 2500 | SRP105410 | HI.4079.001.Index_18.V_R1.fastq.gz HI.4096.008.Index_18.V_R1.fastq.gz | fastq fastq | 2027423200.0 | 20274232.0 | HI.4079.001.Index 18.V R1.fastq.gz | 0:100 1:0 | A:542440452;C:449399743;G:440389586;T:594839784;N:353635 | 100 | 0 | 542440452 | 449399743 | 440389586 | 594839784 | 353635 | SRX2768756 | SRS2152463 | SRA557480 | Brandon University|Biology | Brandon University | 1 | 0.92441 | 0.16974 | 0.6842 | 0.48209 | 100 | B | usable mapping rate | illumina | hiseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | Canada | 2018-09-11 | Undetermined | Multi-stage | Trunk | Surface Structure | |||||||||||||||||||||||||||
| 42219 | 42219 | SRR5485619 | SRX2768755 | SRS2152464 | SRP105410 | PRJNA384462 | Danio rerio Transcriptome or Gene expression | PRJNA384462 | Whole Genome Sequencing | 5 dpf larval zebrafish Danio rerio were exposed to 0 5 and 20mg/L of virgin polyethylene microparticles in dechlorinated water for up to 14 days. Fish were maintained at 26C throughout the experiment and fed at libitum. Whole RNA was extracted from 4 replicates of 35 pooled embryos from each treatment at day 2 and day 14 post exposure with RNeasy kit QIAGEN. The NEB libraries generated from these extracted RNA were then sequenced on 2 Illumina HiSeq 2500 SR 100bp lanes. | W 6.5.14 | ecotype:Growth Chamber|sex:male and female|tissue:Whole body|treatment:6.5.14|age:newborn|BioSampleModel:Model organism or animal | Danio rerio Raw sequence reads | W 6.5.14 | W 6.5.14 | Illumina HiSeq read information to generate gene expression values | WGS | TRANSCRIPTOMIC | RANDOM | SINGLE | ILLUMINA | Illumina HiSeq 2500 | SRP105410 | HI.4079.001.Index_14.W_R1.fastq.gz HI.4096.008.Index_14.W_R1.fastq.gz | fastq fastq | 2139528300.0 | 21395283.0 | HI.4079.001.Index 14.W R1.fastq.gz | 0:100 1:0 | A:569259910;C:478055273;G:469309420;T:622525486;N:378211 | 100 | 0 | 569259910 | 478055273 | 469309420 | 622525486 | 378211 | SRX2768755 | SRS2152464 | SRA557480 | Brandon University|Biology | Brandon University | 1 | 0.92551 | 0.15358 | 0.68136 | 0.47093 | 100 | B | usable mapping rate | illumina | hiseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | Canada | 2018-09-11 | Undetermined | Multi-stage | Trunk | Surface Structure | |||||||||||||||||||||||||||
| 42220 | 42220 | SRR5485618 | SRX2768754 | SRS2152465 | SRP105410 | PRJNA384462 | Danio rerio Transcriptome or Gene expression | PRJNA384462 | Whole Genome Sequencing | 5 dpf larval zebrafish Danio rerio were exposed to 0 5 and 20mg/L of virgin polyethylene microparticles in dechlorinated water for up to 14 days. Fish were maintained at 26C throughout the experiment and fed at libitum. Whole RNA was extracted from 4 replicates of 35 pooled embryos from each treatment at day 2 and day 14 post exposure with RNeasy kit QIAGEN. The NEB libraries generated from these extracted RNA were then sequenced on 2 Illumina HiSeq 2500 SR 100bp lanes. | X 6.20.14 | ecotype:Growth Chamber|sex:male and female|tissue:Whole body|treatment:6.20.14|age:newborn|BioSampleModel:Model organism or animal | Danio rerio Raw sequence reads | X 6.20.14 | X 6.20.14 | Illumina HiSeq read information to generate gene expression values | WGS | TRANSCRIPTOMIC | RANDOM | SINGLE | ILLUMINA | Illumina HiSeq 2500 | SRP105410 | HI.4079.001.Index_16.X_R1.fastq.gz HI.4096.008.Index_16.X_R1.fastq.gz | fastq fastq | 1768110400.0 | 17681104.0 | HI.4079.001.Index 16.X R1.fastq.gz | 0:100 1:0 | A:472810124;C:392555880;G:382616900;T:519817796;N:309700 | 100 | 0 | 472810124 | 392555880 | 382616900 | 519817796 | 309700 | SRX2768754 | SRS2152465 | SRA557480 | Brandon University|Biology | Brandon University | 1 | 0.92486 | 0.16073 | 0.69041 | 0.48403 | 100 | B | usable mapping rate | illumina | hiseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | Canada | 2018-09-11 | Undetermined | Multi-stage | Trunk | Surface Structure | |||||||||||||||||||||||||||
| 53587 | 53587 | SRR11886668 | SRX8434202 | SRS6745285 | SRP265421 | PRJNA559885 | A comparative epigenomic and single cell transcriptomic analysis of teleost regeneration | PRJNA559885 | Other | The ability to regenerate body parts lost to amputation is widely but non uniformly distributed in animals. Species such as bony fishes display extensive regenerative capacities while others such as mammals regenerate poorly. Even though regeneration has been the subject of extensive phylogenetic developmental cellular and molecular studies the mechanisms underlying the broad disparity of regenerative capacities in animals remain elusive. Here we report on a comparative epigenomic and transcriptomic approach which identified an evolutionarily conserved regeneration response program in vertebrates. By defining the cis regulomes and single cell transcriptomes of early stages of regeneration in the distantly related African killifish Nothobranchius furzeri and the zebrafish Danio rerio we uncovered species specific and evolutionarily conserved genomic responses to amputation. | zebrafish fin regeneration | zebrafish fin 1dpa | strain:AB|isolate:12|breed:N/A|cultivar:N/A|ecotype:N/A|age:6 mpf|dev stage:Adult|sex:not collected|tissue:caudal fin|BioSampleModel:Model organism or animal | zebrafish RNAseq 1dpa R3 | L34024 | L34024 | Three biological replicates 6 fish per replicate were prepared for each fin regeneration time point. Ten different African killifish time points were studied including 0dpa 3hpa 6hpa 14hpa 1dpa 2dpa 3dpa 4dpa 7dpa and 18dpa. Samples from 0dpa and 1dpa were collected for zebrafish. All the freshly dissected samples used for RNA seq were quickly rinsed in cold PBS and placed in a 1.5 ml Eppendorf tube followed by flash freezing in liquid nitrogen. Samples were then homogenized with a sterile pestle in 1 ml Trizol reagent Ambion and stored at 80 C until RNA extraction. | RNA-Seq | TRANSCRIPTOMIC | RANDOM | SINGLE | ILLUMINA | Illumina HiSeq 2500 | SRP265421 | ws_3_1_GCCAAT.fastq.gz | fastq | 1907555958.0 | 37403058.0 | ws 3 1 GCCAAT.fastq.gz | 0:51 1:0 | A:417030128;C:470533688;G:468961155;T:550856047;N:174940 | 51 | 0 | 417030128 | 470533688 | 468961155 | 550856047 | 174940 | SRX8434202 | SRS6745285 | SRA1081627 | Stowers Institute for Medical Research|Sanchez lab | Stowers Institute for Medical Research | 1 | 0.96213 | 0.05602 | 0.72967 | 0.45848 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | United States | 2020-05-31 | Adult | Adult | Fin | Surface Structure | ||||||||||||||||||||||||||
| 53588 | 53588 | SRR11886669 | SRX8434201 | SRS6745285 | SRP265421 | PRJNA559885 | A comparative epigenomic and single cell transcriptomic analysis of teleost regeneration | PRJNA559885 | Other | The ability to regenerate body parts lost to amputation is widely but non uniformly distributed in animals. Species such as bony fishes display extensive regenerative capacities while others such as mammals regenerate poorly. Even though regeneration has been the subject of extensive phylogenetic developmental cellular and molecular studies the mechanisms underlying the broad disparity of regenerative capacities in animals remain elusive. Here we report on a comparative epigenomic and transcriptomic approach which identified an evolutionarily conserved regeneration response program in vertebrates. By defining the cis regulomes and single cell transcriptomes of early stages of regeneration in the distantly related African killifish Nothobranchius furzeri and the zebrafish Danio rerio we uncovered species specific and evolutionarily conserved genomic responses to amputation. | zebrafish fin regeneration | zebrafish fin 1dpa | strain:AB|isolate:12|breed:N/A|cultivar:N/A|ecotype:N/A|age:6 mpf|dev stage:Adult|sex:not collected|tissue:caudal fin|BioSampleModel:Model organism or animal | zebrafish RNAseq 1dpa R2 | L34023 | L34023 | Three biological replicates 6 fish per replicate were prepared for each fin regeneration time point. Ten different African killifish time points were studied including 0dpa 3hpa 6hpa 14hpa 1dpa 2dpa 3dpa 4dpa 7dpa and 18dpa. Samples from 0dpa and 1dpa were collected for zebrafish. All the freshly dissected samples used for RNA seq were quickly rinsed in cold PBS and placed in a 1.5 ml Eppendorf tube followed by flash freezing in liquid nitrogen. Samples were then homogenized with a sterile pestle in 1 ml Trizol reagent Ambion and stored at 80 C until RNA extraction. | RNA-Seq | TRANSCRIPTOMIC | RANDOM | SINGLE | ILLUMINA | Illumina HiSeq 2500 | SRP265421 | ws_3_1_ACAGTG.fastq.gz | fastq | 1731933225.0 | 33959475.0 | ws 3 1 ACAGTG.fastq.gz | 0:51 1:0 | A:381487795;C:424246634;G:426026749;T:500012138;N:159909 | 51 | 0 | 381487795 | 424246634 | 426026749 | 500012138 | 159909 | SRX8434201 | SRS6745285 | SRA1081627 | Stowers Institute for Medical Research|Sanchez lab | Stowers Institute for Medical Research | 1 | 0.96269 | 0.05578 | 0.73261 | 0.45837 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | United States | 2020-05-31 | Adult | Adult | Fin | Surface Structure | ||||||||||||||||||||||||||
| 53589 | 53589 | SRR11886671 | SRX8434200 | SRS6745285 | SRP265421 | PRJNA559885 | A comparative epigenomic and single cell transcriptomic analysis of teleost regeneration | PRJNA559885 | Other | The ability to regenerate body parts lost to amputation is widely but non uniformly distributed in animals. Species such as bony fishes display extensive regenerative capacities while others such as mammals regenerate poorly. Even though regeneration has been the subject of extensive phylogenetic developmental cellular and molecular studies the mechanisms underlying the broad disparity of regenerative capacities in animals remain elusive. Here we report on a comparative epigenomic and transcriptomic approach which identified an evolutionarily conserved regeneration response program in vertebrates. By defining the cis regulomes and single cell transcriptomes of early stages of regeneration in the distantly related African killifish Nothobranchius furzeri and the zebrafish Danio rerio we uncovered species specific and evolutionarily conserved genomic responses to amputation. | zebrafish fin regeneration | zebrafish fin 1dpa | strain:AB|isolate:12|breed:N/A|cultivar:N/A|ecotype:N/A|age:6 mpf|dev stage:Adult|sex:not collected|tissue:caudal fin|BioSampleModel:Model organism or animal | zebrafish RNAseq 1dpa R1 | L34022 | L34022 | Three biological replicates 6 fish per replicate were prepared for each fin regeneration time point. Ten different African killifish time points were studied including 0dpa 3hpa 6hpa 14hpa 1dpa 2dpa 3dpa 4dpa 7dpa and 18dpa. Samples from 0dpa and 1dpa were collected for zebrafish. All the freshly dissected samples used for RNA seq were quickly rinsed in cold PBS and placed in a 1.5 ml Eppendorf tube followed by flash freezing in liquid nitrogen. Samples were then homogenized with a sterile pestle in 1 ml Trizol reagent Ambion and stored at 80 C until RNA extraction. | RNA-Seq | TRANSCRIPTOMIC | RANDOM | SINGLE | ILLUMINA | Illumina HiSeq 2500 | SRP265421 | ws_3_1_TGACCA.fastq.gz | fastq | 1814021907.0 | 35569057.0 | ws 3 1 TGACCA.fastq.gz | 0:51 1:0 | A:404693395;C:438897306;G:444600496;T:525663505;N:167205 | 51 | 0 | 404693395 | 438897306 | 444600496 | 525663505 | 167205 | SRX8434200 | SRS6745285 | SRA1081627 | Stowers Institute for Medical Research|Sanchez lab | Stowers Institute for Medical Research | 1 | 0.95686 | 0.06383 | 0.72677 | 0.44564 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | United States | 2020-05-31 | Adult | Adult | Fin | Surface Structure | ||||||||||||||||||||||||||
| 53590 | 53590 | SRR11886672 | SRX8434199 | SRS6745284 | SRP265421 | PRJNA559885 | A comparative epigenomic and single cell transcriptomic analysis of teleost regeneration | PRJNA559885 | Other | The ability to regenerate body parts lost to amputation is widely but non uniformly distributed in animals. Species such as bony fishes display extensive regenerative capacities while others such as mammals regenerate poorly. Even though regeneration has been the subject of extensive phylogenetic developmental cellular and molecular studies the mechanisms underlying the broad disparity of regenerative capacities in animals remain elusive. Here we report on a comparative epigenomic and transcriptomic approach which identified an evolutionarily conserved regeneration response program in vertebrates. By defining the cis regulomes and single cell transcriptomes of early stages of regeneration in the distantly related African killifish Nothobranchius furzeri and the zebrafish Danio rerio we uncovered species specific and evolutionarily conserved genomic responses to amputation. | zebrafish fin regeneration | zebrafish fin 0dpa | strain:AB|isolate:11|breed:N/A|cultivar:N/A|ecotype:N/A|age:6 mpf|dev stage:Adult|sex:not collected|tissue:caudal fin|BioSampleModel:Model organism or animal | zebrafish RNAseq 0dpa R3 | L34021 | L34021 | Three biological replicates 6 fish per replicate were prepared for each fin regeneration time point. Ten different African killifish time points were studied including 0dpa 3hpa 6hpa 14hpa 1dpa 2dpa 3dpa 4dpa 7dpa and 18dpa. Samples from 0dpa and 1dpa were collected for zebrafish. All the freshly dissected samples used for RNA seq were quickly rinsed in cold PBS and placed in a 1.5 ml Eppendorf tube followed by flash freezing in liquid nitrogen. Samples were then homogenized with a sterile pestle in 1 ml Trizol reagent Ambion and stored at 80 C until RNA extraction. | RNA-Seq | TRANSCRIPTOMIC | RANDOM | SINGLE | ILLUMINA | Illumina HiSeq 2500 | SRP265421 | ws_3_1_TTAGGC.fastq.gz | fastq | 1894657395.0 | 37150145.0 | ws 3 1 TTAGGC.fastq.gz | 0:51 1:0 | A:424630121;C:461337738;G:460024108;T:548490887;N:174541 | 51 | 0 | 424630121 | 461337738 | 460024108 | 548490887 | 174541 | SRX8434199 | SRS6745284 | SRA1081627 | Stowers Institute for Medical Research|Sanchez lab | Stowers Institute for Medical Research | 1 | 0.9524 | 0.08535 | 0.70999 | 0.44299 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | United States | 2020-05-31 | Adult | Adult | Fin | Surface Structure | ||||||||||||||||||||||||||
| 53591 | 53591 | SRR11886673 | SRX8434198 | SRS6745284 | SRP265421 | PRJNA559885 | A comparative epigenomic and single cell transcriptomic analysis of teleost regeneration | PRJNA559885 | Other | The ability to regenerate body parts lost to amputation is widely but non uniformly distributed in animals. Species such as bony fishes display extensive regenerative capacities while others such as mammals regenerate poorly. Even though regeneration has been the subject of extensive phylogenetic developmental cellular and molecular studies the mechanisms underlying the broad disparity of regenerative capacities in animals remain elusive. Here we report on a comparative epigenomic and transcriptomic approach which identified an evolutionarily conserved regeneration response program in vertebrates. By defining the cis regulomes and single cell transcriptomes of early stages of regeneration in the distantly related African killifish Nothobranchius furzeri and the zebrafish Danio rerio we uncovered species specific and evolutionarily conserved genomic responses to amputation. | zebrafish fin regeneration | zebrafish fin 0dpa | strain:AB|isolate:11|breed:N/A|cultivar:N/A|ecotype:N/A|age:6 mpf|dev stage:Adult|sex:not collected|tissue:caudal fin|BioSampleModel:Model organism or animal | zebrafish RNAseq 0dpa R2 | L34020 | L34020 | Three biological replicates 6 fish per replicate were prepared for each fin regeneration time point. Ten different African killifish time points were studied including 0dpa 3hpa 6hpa 14hpa 1dpa 2dpa 3dpa 4dpa 7dpa and 18dpa. Samples from 0dpa and 1dpa were collected for zebrafish. All the freshly dissected samples used for RNA seq were quickly rinsed in cold PBS and placed in a 1.5 ml Eppendorf tube followed by flash freezing in liquid nitrogen. Samples were then homogenized with a sterile pestle in 1 ml Trizol reagent Ambion and stored at 80 C until RNA extraction. | RNA-Seq | TRANSCRIPTOMIC | RANDOM | SINGLE | ILLUMINA | Illumina HiSeq 2500 | SRP265421 | ws_3_1_CGATGT.fastq.gz | fastq | 1826016036.0 | 35804236.0 | ws 3 1 CGATGT.fastq.gz | 0:51 1:0 | A:408512993;C:445830968;G:443573205;T:527930321;N:168549 | 51 | 0 | 408512993 | 445830968 | 443573205 | 527930321 | 168549 | SRX8434198 | SRS6745284 | SRA1081627 | Stowers Institute for Medical Research|Sanchez lab | Stowers Institute for Medical Research | 1 | 0.95224 | 0.08373 | 0.70934 | 0.45345 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | United States | 2020-05-31 | Adult | Adult | Fin | Surface Structure | ||||||||||||||||||||||||||
| 53592 | 53592 | SRR11886674 | SRX8434197 | SRS6745284 | SRP265421 | PRJNA559885 | A comparative epigenomic and single cell transcriptomic analysis of teleost regeneration | PRJNA559885 | Other | The ability to regenerate body parts lost to amputation is widely but non uniformly distributed in animals. Species such as bony fishes display extensive regenerative capacities while others such as mammals regenerate poorly. Even though regeneration has been the subject of extensive phylogenetic developmental cellular and molecular studies the mechanisms underlying the broad disparity of regenerative capacities in animals remain elusive. Here we report on a comparative epigenomic and transcriptomic approach which identified an evolutionarily conserved regeneration response program in vertebrates. By defining the cis regulomes and single cell transcriptomes of early stages of regeneration in the distantly related African killifish Nothobranchius furzeri and the zebrafish Danio rerio we uncovered species specific and evolutionarily conserved genomic responses to amputation. | zebrafish fin regeneration | zebrafish fin 0dpa | strain:AB|isolate:11|breed:N/A|cultivar:N/A|ecotype:N/A|age:6 mpf|dev stage:Adult|sex:not collected|tissue:caudal fin|BioSampleModel:Model organism or animal | zebrafish RNAseq 0dpa R1 | L34019 | L34019 | Three biological replicates 6 fish per replicate were prepared for each fin regeneration time point. Ten different African killifish time points were studied including 0dpa 3hpa 6hpa 14hpa 1dpa 2dpa 3dpa 4dpa 7dpa and 18dpa. Samples from 0dpa and 1dpa were collected for zebrafish. All the freshly dissected samples used for RNA seq were quickly rinsed in cold PBS and placed in a 1.5 ml Eppendorf tube followed by flash freezing in liquid nitrogen. Samples were then homogenized with a sterile pestle in 1 ml Trizol reagent Ambion and stored at 80 C until RNA extraction. | RNA-Seq | TRANSCRIPTOMIC | RANDOM | SINGLE | ILLUMINA | Illumina HiSeq 2500 | SRP265421 | ws_3_1_ATCACG.fastq.gz | fastq | 1889698206.0 | 37052906.0 | ws 3 1 ATCACG.fastq.gz | 0:51 1:0 | A:425019592;C:459513661;G:459679307;T:545311781;N:173865 | 51 | 0 | 425019592 | 459513661 | 459679307 | 545311781 | 173865 | SRX8434197 | SRS6745284 | SRA1081627 | Stowers Institute for Medical Research|Sanchez lab | Stowers Institute for Medical Research | 1 | 0.95224 | 0.08995 | 0.71106 | 0.45283 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | United States | 2020-05-31 | Adult | Adult | Fin | Surface Structure | ||||||||||||||||||||||||||
| 53593 | 53593 | SRR11886700 | SRX8434172 | SRS6745285 | SRP265421 | PRJNA559885 | A comparative epigenomic and single cell transcriptomic analysis of teleost regeneration | PRJNA559885 | Other | The ability to regenerate body parts lost to amputation is widely but non uniformly distributed in animals. Species such as bony fishes display extensive regenerative capacities while others such as mammals regenerate poorly. Even though regeneration has been the subject of extensive phylogenetic developmental cellular and molecular studies the mechanisms underlying the broad disparity of regenerative capacities in animals remain elusive. Here we report on a comparative epigenomic and transcriptomic approach which identified an evolutionarily conserved regeneration response program in vertebrates. By defining the cis regulomes and single cell transcriptomes of early stages of regeneration in the distantly related African killifish Nothobranchius furzeri and the zebrafish Danio rerio we uncovered species specific and evolutionarily conserved genomic responses to amputation. | zebrafish fin regeneration | zebrafish fin 1dpa | strain:AB|isolate:12|breed:N/A|cultivar:N/A|ecotype:N/A|age:6 mpf|dev stage:Adult|sex:not collected|tissue:caudal fin|BioSampleModel:Model organism or animal | zebrafish blastema single cell | L41598 | L41598 | The African killifish and zebrafish single cell RNA seq were done with the 10x Chromium platform. About 20 fish were used for cell dissociation in each experiment. Hoechst stained cells 100 000 cells from the dissected blastema tissues at 1dpa were collected on ice using a BD Influx sorter. The viability of cells 94.5% was determined before loading cells into 10x Chromium platform. | RNA-Seq | TRANSCRIPTOMIC SINGLE CELL | RANDOM | PAIRED | ILLUMINA | Illumina HiSeq 2500 | SRP265421 | loader:fastq load.py | L41598_S1_L001_I1_001.fastq.gz L41598_S1_L001_R1_001.fastq.gz L41598_S1_L001_R2_001.fastq.gz L41598_S1_L002_I1_001.fastq.gz L41598_S1_L002_R1_001.fastq.gz L41598_S1_L002_R2_001.fastq.gz L41598_S1_L003_I1_001.fastq.gz L41598_S1_L003_R1_001.fastq.gz L41598_S1_L003_R2_001.fastq.gz L41598_S1_L004_I1_001.fastq.gz L41598_S1_L004_R1_001.fastq.gz L41598_S1_L004_R2_001.fastq.gz | fastq fastq fastq fastq fastq fastq fastq fastq fastq fastq fastq fastq | 52222012142.0 | 411196946.0 | L41598 S1 L001 I1 001.fastq.gz | 0:8 1:28 2:91 | A:10978857897;C:7760934381;G:8557391299;T:10029401940;N:92336569 | 8 | 28 | 91 | 10978857897 | 7760934381 | 8557391299 | 10029401940 | 92336569 | SRX8434172 | SRS6745285 | SRA1081627 | Stowers Institute for Medical Research|Sanchez lab | Stowers Institute for Medical Research | 1 | 0.92629 | 0.12539 | 0.78589 | 0.5225 | 91 | B | usable mapping rate | illumina | hiseq_era | unknown | random_priming | unknown | sc | single_cell_droplet | 10x | United States | 2020-08-11 | Adult | Adult | Fin | Surface Structure | ||||||||||||||||||||||||
| 55982 | 55982 | SRR10895887 | SRX7564592 | SRS6001802 | SRP241982 | PRJNA599026 | Project of basal ray finned fishes | PRJNA599026 | Other | The project is to study the oldest fish lineages in the ray finned fishes. Although they belong to fish their body structure and behavior remain highly similar to that of the tetrapods. Through comparative genome analysis with living vertebrates we provides insights into the molecular basis of terrestrial adaptation of basal ray finned fishes. | pubmed:33545088;pubmed:12470943 | Zebrafish 007 | strain:not collected|isolate:not collected|breed:not collected|cultivar:not collected|ecotype:not collected|age:not collected|dev stage:Adult|sex:not determined|tissue:Skin|BioSampleModel:Model organism or animal | RNA Seq of Danio rerio : adult | CL100101924 L01 9 | CL100101924 L01 9 | RNA-Seq | TRANSCRIPTOMIC | RANDOM | PAIRED | BGISEQ | BGISEQ-500 | SRP241982 | CL100101924_L01_550_1.fq.gz CL100101924_L01_550_2.fq.gz | fastq fastq | 8780226200.0 | 87802262.0 | CL100101924 L01 550 1.fq.gz | 0:100 1:100 | A:2363664067;C:1983383192;G:2025795704;T:2396528723;N:10854514 | 100 | 100 | 2363664067 | 1983383192 | 2025795704 | 2396528723 | 10854514 | SRX7564592 | SRS6001802 | SRA1026516 | BGI|BGI-Research | BGI | 1 | 0.92686 | 0.10085 | 0.70903 | 0.51488 | 100 | B | usable mapping rate | bgi | bgi | unknown | random_priming | unknown | bulk | unknown | unknown | China | 2020-01-15 | Adult | Adult | Skin | Surface Structure | |||||||||||||||||||||||||||
| 55983 | 55983 | SRR10895888 | SRX7564591 | SRS6001802 | SRP241982 | PRJNA599026 | Project of basal ray finned fishes | PRJNA599026 | Other | The project is to study the oldest fish lineages in the ray finned fishes. Although they belong to fish their body structure and behavior remain highly similar to that of the tetrapods. Through comparative genome analysis with living vertebrates we provides insights into the molecular basis of terrestrial adaptation of basal ray finned fishes. | pubmed:33545088;pubmed:12470943 | Zebrafish 007 | strain:not collected|isolate:not collected|breed:not collected|cultivar:not collected|ecotype:not collected|age:not collected|dev stage:Adult|sex:not determined|tissue:Skin|BioSampleModel:Model organism or animal | RNA Seq of Danio rerio : adult | CL100101924 L01 8 | CL100101924 L01 8 | RNA-Seq | TRANSCRIPTOMIC | RANDOM | PAIRED | BGISEQ | BGISEQ-500 | SRP241982 | CL100101924_L01_549_2.fq.gz CL100101924_L01_549_1.fq.gz | fastq fastq | 9805051200.0 | 98050512.0 | CL100101924 L01 549 1.fq.gz | 0:100 1:100 | A:2663706429;C:2193838939;G:2241619945;T:2693859046;N:12026841 | 100 | 100 | 2663706429 | 2193838939 | 2241619945 | 2693859046 | 12026841 | SRX7564591 | SRS6001802 | SRA1026516 | BGI|BGI-Research | BGI | 1 | 0.92652 | 0.0983 | 0.71043 | 0.53401 | 100 | B | usable mapping rate | bgi | bgi | unknown | random_priming | unknown | bulk | unknown | unknown | China | 2020-01-15 | Adult | Adult | Skin | Surface Structure | |||||||||||||||||||||||||||
| 55984 | 55984 | SRR10895889 | SRX7564590 | SRS6001802 | SRP241982 | PRJNA599026 | Project of basal ray finned fishes | PRJNA599026 | Other | The project is to study the oldest fish lineages in the ray finned fishes. Although they belong to fish their body structure and behavior remain highly similar to that of the tetrapods. Through comparative genome analysis with living vertebrates we provides insights into the molecular basis of terrestrial adaptation of basal ray finned fishes. | pubmed:33545088;pubmed:12470943 | Zebrafish 007 | strain:not collected|isolate:not collected|breed:not collected|cultivar:not collected|ecotype:not collected|age:not collected|dev stage:Adult|sex:not determined|tissue:Skin|BioSampleModel:Model organism or animal | RNA Seq of Danio rerio : adult | CL100101924 L01 7 | CL100101924 L01 7 | RNA-Seq | TRANSCRIPTOMIC | RANDOM | PAIRED | BGISEQ | BGISEQ-500 | SRP241982 | CL100101924_L01_547_1.fq.gz CL100101924_L01_547_2.fq.gz | fastq fastq | 9137587600.0 | 91375876.0 | CL100101924 L01 547 1.fq.gz | 0:100 1:100 | A:2471506783;C:2052808667;G:2096159921;T:2505897469;N:11214760 | 100 | 100 | 2471506783 | 2052808667 | 2096159921 | 2505897469 | 11214760 | SRX7564590 | SRS6001802 | SRA1026516 | BGI|BGI-Research | BGI | 1 | 0.93126 | 0.09864 | 0.70613 | 0.51947 | 100 | B | usable mapping rate | bgi | bgi | unknown | random_priming | unknown | bulk | unknown | unknown | China | 2020-01-15 | Adult | Adult | Skin | Surface Structure | |||||||||||||||||||||||||||
| 55985 | 55985 | SRR10895890 | SRX7564589 | SRS6001802 | SRP241982 | PRJNA599026 | Project of basal ray finned fishes | PRJNA599026 | Other | The project is to study the oldest fish lineages in the ray finned fishes. Although they belong to fish their body structure and behavior remain highly similar to that of the tetrapods. Through comparative genome analysis with living vertebrates we provides insights into the molecular basis of terrestrial adaptation of basal ray finned fishes. | pubmed:33545088;pubmed:12470943 | Zebrafish 007 | strain:not collected|isolate:not collected|breed:not collected|cultivar:not collected|ecotype:not collected|age:not collected|dev stage:Adult|sex:not determined|tissue:Skin|BioSampleModel:Model organism or animal | RNA Seq of Danio rerio : adult | CL100101924 L01 6 | CL100101924 L01 6 | RNA-Seq | TRANSCRIPTOMIC | RANDOM | PAIRED | BGISEQ | BGISEQ-500 | SRP241982 | CL100101924_L01_551_1.fq.gz CL100101924_L01_551_2.fq.gz | fastq fastq | 7665129600.0 | 76651296.0 | CL100101924 L01 551 1.fq.gz | 0:100 1:100 | A:2062939973;C:1731950056;G:1768675635;T:2092110510;N:9453426 | 100 | 100 | 2062939973 | 1731950056 | 1768675635 | 2092110510 | 9453426 | SRX7564589 | SRS6001802 | SRA1026516 | BGI|BGI-Research | BGI | 1 | 0.93022 | 0.10662 | 0.70512 | 0.50773 | 100 | B | usable mapping rate | bgi | bgi | unknown | random_priming | unknown | bulk | unknown | unknown | China | 2020-01-15 | Adult | Adult | Skin | Surface Structure | |||||||||||||||||||||||||||
| 55986 | 55986 | SRR10895892 | SRX7564587 | SRS6001802 | SRP241982 | PRJNA599026 | Project of basal ray finned fishes | PRJNA599026 | Other | The project is to study the oldest fish lineages in the ray finned fishes. Although they belong to fish their body structure and behavior remain highly similar to that of the tetrapods. Through comparative genome analysis with living vertebrates we provides insights into the molecular basis of terrestrial adaptation of basal ray finned fishes. | pubmed:33545088;pubmed:12470943 | Zebrafish 007 | strain:not collected|isolate:not collected|breed:not collected|cultivar:not collected|ecotype:not collected|age:not collected|dev stage:Adult|sex:not determined|tissue:Skin|BioSampleModel:Model organism or animal | RNA Seq of Danio rerio : adult | CL100101924 L01 5 | CL100101924 L01 5 | RNA-Seq | TRANSCRIPTOMIC | RANDOM | PAIRED | BGISEQ | BGISEQ-500 | SRP241982 | CL100101924_L01_548_1.fq.gz CL100101924_L01_548_2.fq.gz | fastq fastq | 6527814200.0 | 65278142.0 | CL100101924 L01 548 1.fq.gz | 0:100 1:100 | A:1748761735;C:1483522306;G:1516468410;T:1771028617;N:8033132 | 100 | 100 | 1748761735 | 1483522306 | 1516468410 | 1771028617 | 8033132 | SRX7564587 | SRS6001802 | SRA1026516 | BGI|BGI-Research | BGI | 1 | 0.93149 | 0.09371 | 0.70542 | 0.51664 | 100 | B | usable mapping rate | bgi | bgi | unknown | random_priming | unknown | bulk | unknown | unknown | China | 2020-01-15 | Adult | Adult | Skin | Surface Structure | |||||||||||||||||||||||||||
| 56002 | 56002 | SRR10895909 | SRX7564570 | SRS6001798 | SRP241982 | PRJNA599026 | Project of basal ray finned fishes | PRJNA599026 | Other | The project is to study the oldest fish lineages in the ray finned fishes. Although they belong to fish their body structure and behavior remain highly similar to that of the tetrapods. Through comparative genome analysis with living vertebrates we provides insights into the molecular basis of terrestrial adaptation of basal ray finned fishes. | pubmed:33545088;pubmed:12470943 | Zebrafish 003 | strain:not collected|isolate:not collected|breed:not collected|cultivar:not collected|ecotype:not collected|age:not collected|dev stage:Adult|sex:not determined|tissue:Jaw|BioSampleModel:Model organism or animal | RNA Seq of Danio rerio : adult | CL100103858 L02 6 | CL100103858 L02 6 | RNA-Seq | TRANSCRIPTOMIC | RANDOM | PAIRED | BGISEQ | BGISEQ-500 | SRP241982 | CL100103858_L02_524_1.fq.gz CL100103858_L02_524_2.fq.gz | fastq fastq | 8863838800.0 | 88638388.0 | CL100103858 L02 524 1.fq.gz | 0:100 1:100 | A:2346347928;C:2037236505;G:2089998345;T:2378150886;N:12105136 | 100 | 100 | 2346347928 | 2037236505 | 2089998345 | 2378150886 | 12105136 | SRX7564570 | SRS6001798 | SRA1026516 | BGI|BGI-Research | BGI | 1 | 0.93198 | 0.08128 | 0.74519 | 0.48951 | 100 | B | usable mapping rate | bgi | bgi | unknown | random_priming | unknown | bulk | unknown | unknown | China | 2020-01-15 | Adult | Adult | Jaw | Surface Structure | |||||||||||||||||||||||||||
| 56003 | 56003 | SRR10895910 | SRX7564569 | SRS6001798 | SRP241982 | PRJNA599026 | Project of basal ray finned fishes | PRJNA599026 | Other | The project is to study the oldest fish lineages in the ray finned fishes. Although they belong to fish their body structure and behavior remain highly similar to that of the tetrapods. Through comparative genome analysis with living vertebrates we provides insights into the molecular basis of terrestrial adaptation of basal ray finned fishes. | pubmed:33545088;pubmed:12470943 | Zebrafish 003 | strain:not collected|isolate:not collected|breed:not collected|cultivar:not collected|ecotype:not collected|age:not collected|dev stage:Adult|sex:not determined|tissue:Jaw|BioSampleModel:Model organism or animal | RNA Seq of Danio rerio : adult | CL100103858 L02 5 | CL100103858 L02 5 | RNA-Seq | TRANSCRIPTOMIC | RANDOM | PAIRED | BGISEQ | BGISEQ-500 | SRP241982 | CL100103858_L02_521_2.fq.gz CL100103858_L02_521_1.fq.gz | fastq fastq | 10301853000.0 | 103018530.0 | CL100103858 L02 521 1.fq.gz | 0:100 1:100 | A:2719789797;C:2379371302;G:2435354849;T:2753122052;N:14215000 | 100 | 100 | 2719789797 | 2379371302 | 2435354849 | 2753122052 | 14215000 | SRX7564569 | SRS6001798 | SRA1026516 | BGI|BGI-Research | BGI | 1 | 0.93582 | 0.08016 | 0.73466 | 0.4997 | 100 | B | usable mapping rate | bgi | bgi | unknown | random_priming | unknown | bulk | unknown | unknown | China | 2020-01-15 | Adult | Adult | Jaw | Surface Structure | |||||||||||||||||||||||||||
| 56004 | 56004 | SRR10895911 | SRX7564568 | SRS6001798 | SRP241982 | PRJNA599026 | Project of basal ray finned fishes | PRJNA599026 | Other | The project is to study the oldest fish lineages in the ray finned fishes. Although they belong to fish their body structure and behavior remain highly similar to that of the tetrapods. Through comparative genome analysis with living vertebrates we provides insights into the molecular basis of terrestrial adaptation of basal ray finned fishes. | pubmed:33545088;pubmed:12470943 | Zebrafish 003 | strain:not collected|isolate:not collected|breed:not collected|cultivar:not collected|ecotype:not collected|age:not collected|dev stage:Adult|sex:not determined|tissue:Jaw|BioSampleModel:Model organism or animal | RNA Seq of Danio rerio : adult | CL100103858 L02 4 | CL100103858 L02 4 | RNA-Seq | TRANSCRIPTOMIC | RANDOM | PAIRED | BGISEQ | BGISEQ-500 | SRP241982 | CL100103858_L02_522_1.fq.gz CL100103858_L02_522_2.fq.gz | fastq fastq | 8856151600.0 | 88561516.0 | CL100103858 L02 522 1.fq.gz | 0:100 1:100 | A:2327973616;C:2055012813;G:2104952956;T:2356113813;N:12098402 | 100 | 100 | 2327973616 | 2055012813 | 2104952956 | 2356113813 | 12098402 | SRX7564568 | SRS6001798 | SRA1026516 | BGI|BGI-Research | BGI | 1 | 0.93888 | 0.07312 | 0.74337 | 0.50608 | 100 | B | usable mapping rate | bgi | bgi | unknown | random_priming | unknown | bulk | unknown | unknown | China | 2020-01-15 | Adult | Adult | Jaw | Surface Structure | |||||||||||||||||||||||||||
| 56005 | 56005 | SRR10895912 | SRX7564567 | SRS6001798 | SRP241982 | PRJNA599026 | Project of basal ray finned fishes | PRJNA599026 | Other | The project is to study the oldest fish lineages in the ray finned fishes. Although they belong to fish their body structure and behavior remain highly similar to that of the tetrapods. Through comparative genome analysis with living vertebrates we provides insights into the molecular basis of terrestrial adaptation of basal ray finned fishes. | pubmed:33545088;pubmed:12470943 | Zebrafish 003 | strain:not collected|isolate:not collected|breed:not collected|cultivar:not collected|ecotype:not collected|age:not collected|dev stage:Adult|sex:not determined|tissue:Jaw|BioSampleModel:Model organism or animal | RNA Seq of Danio rerio : adult | CL100103858 L02 3 | CL100103858 L02 3 | RNA-Seq | TRANSCRIPTOMIC | RANDOM | PAIRED | BGISEQ | BGISEQ-500 | SRP241982 | CL100103858_L02_525_1.fq.gz CL100103858_L02_525_2.fq.gz | fastq fastq | 8393966200.0 | 83939662.0 | CL100103858 L02 525 1.fq.gz | 0:100 1:100 | A:2205916604;C:1944354784;G:1992482736;T:2239434609;N:11777467 | 100 | 100 | 2205916604 | 1944354784 | 1992482736 | 2239434609 | 11777467 | SRX7564567 | SRS6001798 | SRA1026516 | BGI|BGI-Research | BGI | 1 | 0.93522 | 0.07124 | 0.73939 | 0.50385 | 100 | B | usable mapping rate | bgi | bgi | unknown | random_priming | unknown | bulk | unknown | unknown | China | 2020-01-15 | Adult | Adult | Jaw | Surface Structure | |||||||||||||||||||||||||||
| 56006 | 56006 | SRR10895914 | SRX7564565 | SRS6001798 | SRP241982 | PRJNA599026 | Project of basal ray finned fishes | PRJNA599026 | Other | The project is to study the oldest fish lineages in the ray finned fishes. Although they belong to fish their body structure and behavior remain highly similar to that of the tetrapods. Through comparative genome analysis with living vertebrates we provides insights into the molecular basis of terrestrial adaptation of basal ray finned fishes. | pubmed:33545088;pubmed:12470943 | Zebrafish 003 | strain:not collected|isolate:not collected|breed:not collected|cultivar:not collected|ecotype:not collected|age:not collected|dev stage:Adult|sex:not determined|tissue:Jaw|BioSampleModel:Model organism or animal | RNA Seq of Danio rerio : adult | CL100103858 L02 2 | CL100103858 L02 2 | RNA-Seq | TRANSCRIPTOMIC | RANDOM | PAIRED | BGISEQ | BGISEQ-500 | SRP241982 | CL100103858_L02_523_2.fq.gz CL100103858_L02_523_1.fq.gz | fastq fastq | 9645085400.0 | 96450854.0 | CL100103858 L02 523 1.fq.gz | 0:100 1:100 | A:2543300716;C:2229112849;G:2282624833;T:2576502560;N:13544442 | 100 | 100 | 2543300716 | 2229112849 | 2282624833 | 2576502560 | 13544442 | SRX7564565 | SRS6001798 | SRA1026516 | BGI|BGI-Research | BGI | 1 | 0.93673 | 0.0738 | 0.74337 | 0.49427 | 100 | B | usable mapping rate | bgi | bgi | unknown | random_priming | unknown | bulk | unknown | unknown | China | 2020-01-15 | Adult | Adult | Jaw | Surface Structure | |||||||||||||||||||||||||||
| 58983 | 58983 | SRR11610427 | SRX8176695 | SRS6536450 | SRP258542 | PRJNA626778 | Transcriptome sequencing for larvae and adult zebrafish liver post difenoconazole exposure | PRJNA626778 | Metagenomics | RNA seq was performed on zebrafish larvae post embryos exposed to 0.400 mg/L difenoconazole for 120 h and on zebrafish liver post 21 d exposure in triplicate using Illumina HiSeq X Ten | T06 | cultivar:WT type AB strain|age:10 days|dev stage:larvae|sex:male|tissue:larvae whole body|treatment:D larvae3|BioSampleModel:Model organism or animal | NS 3 | D larvae3 | D larvae3 | D larvae3 | RNA-Seq | TRANSCRIPTOMIC | RANDOM | PAIRED | ILLUMINA | HiSeq X Ten | SRP258542 | T06_2.fq.gz T06_1.fq.gz | fastq fastq | 8705370600.0 | 29017902.0 | T06 1.fq.gz | 0:150 1:150 | A:2317335170;C:2020864131;G:2032474454;T:2333792505;N:904340 | 150 | 150 | 2317335170 | 2020864131 | 2032474454 | 2333792505 | 904340 | SRX8176695 | SRS6536450 | SRA1069066 | Zhejiang Academy of Agricultural Sciences|Institute of Quality and Standard for Agroproducts | Zhejiang Academy of Agricultural Sciences | 2 | 0.94348 | 0.94914 | 0.10329 | 0.1021 | 0.65163 | 0.6524 | 0.47004 | 0.46491 | 150 | 150 | B | B | biological fallback assumption | illumina | hiseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | China | 2020-04-26 | Larval | Larval | Trunk | Surface Structure | |||||||||||||||||||||
| 58984 | 58984 | SRR11610428 | SRX8176694 | SRS6536449 | SRP258542 | PRJNA626778 | Transcriptome sequencing for larvae and adult zebrafish liver post difenoconazole exposure | PRJNA626778 | Metagenomics | RNA seq was performed on zebrafish larvae post embryos exposed to 0.400 mg/L difenoconazole for 120 h and on zebrafish liver post 21 d exposure in triplicate using Illumina HiSeq X Ten | T05 | cultivar:WT type AB strain|age:9 days|dev stage:larvae|sex:male|tissue:larvae whole body|treatment:D larvae2|BioSampleModel:Model organism or animal | NS 2 | D larvae2 | D larvae2 | D larvae2 | RNA-Seq | TRANSCRIPTOMIC | RANDOM | PAIRED | ILLUMINA | HiSeq X Ten | SRP258542 | T05_2.fq.gz T05_1.fq.gz | fastq fastq | 8775682200.0 | 29252274.0 | T05 1.fq.gz | 0:150 1:150 | A:2343850546;C:2028804671;G:2042371366;T:2359745584;N:910033 | 150 | 150 | 2343850546 | 2028804671 | 2042371366 | 2359745584 | 910033 | SRX8176694 | SRS6536449 | SRA1069066 | Zhejiang Academy of Agricultural Sciences|Institute of Quality and Standard for Agroproducts | Zhejiang Academy of Agricultural Sciences | 2 | 0.94324 | 0.9478 | 0.10742 | 0.10626 | 0.65374 | 0.65423 | 0.46936 | 0.47023 | 150 | 150 | B | B | biological fallback assumption | illumina | hiseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | China | 2020-04-26 | Larval | Larval | Trunk | Surface Structure | |||||||||||||||||||||
| 58985 | 58985 | SRR11610429 | SRX8176693 | SRS6536448 | SRP258542 | PRJNA626778 | Transcriptome sequencing for larvae and adult zebrafish liver post difenoconazole exposure | PRJNA626778 | Metagenomics | RNA seq was performed on zebrafish larvae post embryos exposed to 0.400 mg/L difenoconazole for 120 h and on zebrafish liver post 21 d exposure in triplicate using Illumina HiSeq X Ten | T04 | cultivar:WT type AB strain|age:8 days|dev stage:larvae|sex:male|tissue:larvae whole body|treatment:D larvae1|BioSampleModel:Model organism or animal | NS 1 | D larvae1 | D larvae1 | D larvae1 | RNA-Seq | TRANSCRIPTOMIC | RANDOM | PAIRED | ILLUMINA | HiSeq X Ten | SRP258542 | T04_1.fq.gz T04_2.fq.gz | fastq fastq | 9470209800.0 | 31567366.0 | T04 1.fq.gz | 0:150 1:150 | A:2512151104;C:2206180833;G:2224120159;T:2526778146;N:979558 | 150 | 150 | 2512151104 | 2206180833 | 2224120159 | 2526778146 | 979558 | SRX8176693 | SRS6536448 | SRA1069066 | Zhejiang Academy of Agricultural Sciences|Institute of Quality and Standard for Agroproducts | Zhejiang Academy of Agricultural Sciences | 2 | 0.94469 | 0.94918 | 0.09968 | 0.09817 | 0.65224 | 0.6538 | 0.45967 | 0.46068 | 150 | 150 | B | B | biological fallback assumption | illumina | hiseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | China | 2020-04-26 | Larval | Larval | Trunk | Surface Structure | |||||||||||||||||||||
| 58986 | 58986 | SRR11610430 | SRX8176692 | SRS6536447 | SRP258542 | PRJNA626778 | Transcriptome sequencing for larvae and adult zebrafish liver post difenoconazole exposure | PRJNA626778 | Metagenomics | RNA seq was performed on zebrafish larvae post embryos exposed to 0.400 mg/L difenoconazole for 120 h and on zebrafish liver post 21 d exposure in triplicate using Illumina HiSeq X Ten | T03 | cultivar:WT type AB strain|age:7 days|dev stage:larvae|sex:male|tissue:larvae whole body|treatment:ck larvae3|BioSampleModel:Model organism or animal | LS 3 | ck larvae3 | ck larvae3 | ck larvae3 | RNA-Seq | TRANSCRIPTOMIC | RANDOM | PAIRED | ILLUMINA | HiSeq X Ten | SRP258542 | T03_1.fq.gz T03_2.fq.gz | fastq fastq | 8454791400.0 | 28182638.0 | T03 1.fq.gz | 0:150 1:150 | A:2264436569;C:1947544159;G:1962858262;T:2279085384;N:867026 | 150 | 150 | 2264436569 | 1947544159 | 1962858262 | 2279085384 | 867026 | SRX8176692 | SRS6536447 | SRA1069066 | Zhejiang Academy of Agricultural Sciences|Institute of Quality and Standard for Agroproducts | Zhejiang Academy of Agricultural Sciences | 2 | 0.94312 | 0.94697 | 0.11012 | 0.10792 | 0.65042 | 0.65017 | 0.47003 | 0.47097 | 150 | 150 | B | B | biological fallback assumption | illumina | hiseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | China | 2020-04-26 | Larval | Larval | Trunk | Surface Structure | |||||||||||||||||||||
| 58989 | 58989 | SRR11610433 | SRX8176689 | SRS6536445 | SRP258542 | PRJNA626778 | Transcriptome sequencing for larvae and adult zebrafish liver post difenoconazole exposure | PRJNA626778 | Metagenomics | RNA seq was performed on zebrafish larvae post embryos exposed to 0.400 mg/L difenoconazole for 120 h and on zebrafish liver post 21 d exposure in triplicate using Illumina HiSeq X Ten | T02 | cultivar:WT type AB strain|age:6 days|dev stage:larvae|sex:male|tissue:larvae whole body|treatment:ck larvae2|BioSampleModel:Model organism or animal | LS 2 | ck larvae2 | ck larvae2 | ck larvae2 | RNA-Seq | TRANSCRIPTOMIC | RANDOM | PAIRED | ILLUMINA | HiSeq X Ten | SRP258542 | T02_1.fq.gz T02_2.fq.gz | fastq fastq | 9808202700.0 | 32694009.0 | T02 1.fq.gz | 0:150 1:150 | A:2611961476;C:2273571725;G:2295489444;T:2626158782;N:1021273 | 150 | 150 | 2611961476 | 2273571725 | 2295489444 | 2626158782 | 1021273 | SRX8176689 | SRS6536445 | SRA1069066 | Zhejiang Academy of Agricultural Sciences|Institute of Quality and Standard for Agroproducts | Zhejiang Academy of Agricultural Sciences | 2 | 0.94301 | 0.94782 | 0.10296 | 0.10114 | 0.65111 | 0.6507 | 0.47143 | 0.46806 | 150 | 150 | B | B | biological fallback assumption | illumina | hiseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | China | 2020-04-26 | Larval | Larval | Trunk | Surface Structure | |||||||||||||||||||||
| 58990 | 58990 | SRR11610434 | SRX8176688 | SRS6536444 | SRP258542 | PRJNA626778 | Transcriptome sequencing for larvae and adult zebrafish liver post difenoconazole exposure | PRJNA626778 | Metagenomics | RNA seq was performed on zebrafish larvae post embryos exposed to 0.400 mg/L difenoconazole for 120 h and on zebrafish liver post 21 d exposure in triplicate using Illumina HiSeq X Ten | T01 | cultivar:WT type AB strain|age:5 days|dev stage:larvae|sex:male|tissue:larvae whole body|treatment:ck larvae1|BioSampleModel:Model organism or animal | LS 1 | ck larvae1 | ck larvae1 | ck larvae1 | RNA-Seq | TRANSCRIPTOMIC | RANDOM | PAIRED | ILLUMINA | HiSeq X Ten | SRP258542 | T01_1.fq.gz T01_2.fq.gz | fastq fastq | 8337627600.0 | 27792092.0 | T01 1.fq.gz | 0:150 1:150 | A:2205115835;C:1947292476;G:1965213034;T:2219138382;N:867873 | 150 | 150 | 2205115835 | 1947292476 | 1965213034 | 2219138382 | 867873 | SRX8176688 | SRS6536444 | SRA1069066 | Zhejiang Academy of Agricultural Sciences|Institute of Quality and Standard for Agroproducts | Zhejiang Academy of Agricultural Sciences | 2 | 0.94524 | 0.94926 | 0.09868 | 0.09692 | 0.65251 | 0.65399 | 0.47004 | 0.46218 | 150 | 150 | B | B | biological fallback assumption | illumina | hiseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | China | 2020-04-26 | Larval | Larval | Trunk | Surface Structure | |||||||||||||||||||||
| 66804 | 66804 | SRR16647484 | SRX12848204 | SRS10791664 | SRP343938 | PRJNA776643 | RNAseq study for assessed the microcystin LR and geosmin differential gene expression modulation in zebrafish larvae 30 dph | PRJNA776643 | Other | Microcystin LR and Geosmin effects over whole body zebrafish Danio rerio larvae transcriptome | Cyanotoxins exposition | Cyanotoxins | strain:Wild|dev stage:31 dph|sex:Not collected|tissue:whole body|BioSampleModel:Model organism or animal | Geosmin Group | Geosmin5 | Geosmin5 | TruSeq stranded mRNA library | RNA-Seq | TRANSCRIPTOMIC | RANDOM | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP343938 | Geos_9_2.fastq.gz Geos_9_1.fastq.gz | fastq fastq | 6334215404.0 | 31357502.0 | Geos 9 1.fastq.gz | 0:101 1:101 | A:1660680129;C:1502146962;G:1509550327;T:1661764270;N:73716 | 101 | 101 | 1660680129 | 1502146962 | 1509550327 | 1661764270 | 73716 | SRX12848204 | SRS10791664 | SRA1320158 | University of Chile|INTA | University of Chile | 2 | 0.9606 | 0.96446 | 0.06247 | 0.06142 | 0.68024 | 0.67868 | 0.46945 | 0.48301 | 101 | 101 | B | B | biological fallback assumption | illumina | novaseq_era | unknown | random_priming | trueseq | bulk | unknown | unknown | Chile | 2021-10-31 | Undetermined | Larval | Trunk | Surface Structure | ||||||||||||||||||||
| 66805 | 66805 | SRR16647485 | SRX12848203 | SRS10791664 | SRP343938 | PRJNA776643 | RNAseq study for assessed the microcystin LR and geosmin differential gene expression modulation in zebrafish larvae 30 dph | PRJNA776643 | Other | Microcystin LR and Geosmin effects over whole body zebrafish Danio rerio larvae transcriptome | Cyanotoxins exposition | Cyanotoxins | strain:Wild|dev stage:31 dph|sex:Not collected|tissue:whole body|BioSampleModel:Model organism or animal | Geosmin Group | Geosmin4 | Geosmin4 | TruSeq stranded mRNA library | RNA-Seq | TRANSCRIPTOMIC | RANDOM | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP343938 | Geos_8_2.fastq.gz Geos_8_1.fastq.gz | fastq fastq | 4083342938.0 | 20214569.0 | Geos 8 1.fastq.gz | 0:101 1:101 | A:1059060815;C:981147486;G:980661533;T:1062425066;N:48038 | 101 | 101 | 1059060815 | 981147486 | 980661533 | 1062425066 | 48038 | SRX12848203 | SRS10791664 | SRA1320158 | University of Chile|INTA | University of Chile | 2 | 0.95947 | 0.96443 | 0.05387 | 0.05331 | 0.66395 | 0.66419 | 0.49093 | 0.48488 | 101 | 101 | B | B | biological fallback assumption | illumina | novaseq_era | unknown | random_priming | trueseq | bulk | unknown | unknown | Chile | 2021-10-31 | Undetermined | Larval | Trunk | Surface Structure | ||||||||||||||||||||
| 66806 | 66806 | SRR16647486 | SRX12848202 | SRS10791664 | SRP343938 | PRJNA776643 | RNAseq study for assessed the microcystin LR and geosmin differential gene expression modulation in zebrafish larvae 30 dph | PRJNA776643 | Other | Microcystin LR and Geosmin effects over whole body zebrafish Danio rerio larvae transcriptome | Cyanotoxins exposition | Cyanotoxins | strain:Wild|dev stage:31 dph|sex:Not collected|tissue:whole body|BioSampleModel:Model organism or animal | Geosmin Group | Geosmin3 | Geosmin3 | TruSeq stranded mRNA library | RNA-Seq | TRANSCRIPTOMIC | RANDOM | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP343938 | Geos_7_1.fastq.gz Geos_7_2.fastq.gz | fastq fastq | 6351796272.0 | 31444536.0 | Geos 7 1.fastq.gz | 0:101 1:101 | A:1666018762;C:1507166887;G:1508718223;T:1669818164;N:74236 | 101 | 101 | 1666018762 | 1507166887 | 1508718223 | 1669818164 | 74236 | SRX12848202 | SRS10791664 | SRA1320158 | University of Chile|INTA | University of Chile | 2 | 0.95901 | 0.96323 | 0.05991 | 0.05856 | 0.66703 | 0.6663 | 0.47986 | 0.48189 | 101 | 101 | B | B | biological fallback assumption | illumina | novaseq_era | unknown | random_priming | trueseq | bulk | unknown | unknown | Chile | 2021-10-31 | Undetermined | Larval | Trunk | Surface Structure | ||||||||||||||||||||
| 66807 | 66807 | SRR16647487 | SRX12848201 | SRS10791664 | SRP343938 | PRJNA776643 | RNAseq study for assessed the microcystin LR and geosmin differential gene expression modulation in zebrafish larvae 30 dph | PRJNA776643 | Other | Microcystin LR and Geosmin effects over whole body zebrafish Danio rerio larvae transcriptome | Cyanotoxins exposition | Cyanotoxins | strain:Wild|dev stage:31 dph|sex:Not collected|tissue:whole body|BioSampleModel:Model organism or animal | Geosmin Group | Geosmin2 | Geosmin2 | TruSeq stranded mRNA library | RNA-Seq | TRANSCRIPTOMIC | RANDOM | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP343938 | Geos_5_2.fastq.gz Geos_5_1.fastq.gz | fastq fastq | 5034600934.0 | 24923767.0 | Geos 5 1.fastq.gz | 0:101 1:101 | A:1324246402;C:1190124296;G:1192268426;T:1327901769;N:60041 | 101 | 101 | 1324246402 | 1190124296 | 1192268426 | 1327901769 | 60041 | SRX12848201 | SRS10791664 | SRA1320158 | University of Chile|INTA | University of Chile | 2 | 0.95626 | 0.96096 | 0.06801 | 0.06708 | 0.67292 | 0.67109 | 0.47788 | 0.48168 | 101 | 101 | B | B | biological fallback assumption | illumina | novaseq_era | unknown | random_priming | trueseq | bulk | unknown | unknown | Chile | 2021-10-31 | Undetermined | Larval | Trunk | Surface Structure | ||||||||||||||||||||
| 66808 | 66808 | SRR16647488 | SRX12848200 | SRS10791664 | SRP343938 | PRJNA776643 | RNAseq study for assessed the microcystin LR and geosmin differential gene expression modulation in zebrafish larvae 30 dph | PRJNA776643 | Other | Microcystin LR and Geosmin effects over whole body zebrafish Danio rerio larvae transcriptome | Cyanotoxins exposition | Cyanotoxins | strain:Wild|dev stage:31 dph|sex:Not collected|tissue:whole body|BioSampleModel:Model organism or animal | Geosmin Group | Geosmin1 | Geosmin1 | TruSeq stranded mRNA library | RNA-Seq | TRANSCRIPTOMIC | RANDOM | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP343938 | Geos_2_1.fastq.gz Geos_2_2.fastq.gz | fastq fastq | 5540468928.0 | 27428064.0 | Geos 2 1.fastq.gz | 0:101 1:101 | A:1453397068;C:1314496546;G:1318965254;T:1453545221;N:64839 | 101 | 101 | 1453397068 | 1314496546 | 1318965254 | 1453545221 | 64839 | SRX12848200 | SRS10791664 | SRA1320158 | University of Chile|INTA | University of Chile | 2 | 0.96164 | 0.96533 | 0.0572 | 0.056 | 0.68712 | 0.68682 | 0.47672 | 0.48134 | 101 | 101 | B | B | biological fallback assumption | illumina | novaseq_era | unknown | random_priming | trueseq | bulk | unknown | unknown | Chile | 2021-10-31 | Undetermined | Larval | Trunk | Surface Structure | ||||||||||||||||||||
| 66809 | 66809 | SRR16647489 | SRX12848199 | SRS10791664 | SRP343938 | PRJNA776643 | RNAseq study for assessed the microcystin LR and geosmin differential gene expression modulation in zebrafish larvae 30 dph | PRJNA776643 | Other | Microcystin LR and Geosmin effects over whole body zebrafish Danio rerio larvae transcriptome | Cyanotoxins exposition | Cyanotoxins | strain:Wild|dev stage:31 dph|sex:Not collected|tissue:whole body|BioSampleModel:Model organism or animal | Control Group | Control5 | Control5 | TruSeq stranded mRNA library | RNA-Seq | TRANSCRIPTOMIC | RANDOM | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP343938 | Control_10_2.fastq.gz Control_10_1.fastq.gz | fastq fastq | 4443325118.0 | 21996659.0 | Control 10 1.fastq.gz | 0:101 1:101 | A:1166167313;C:1050165932;G:1062683912;T:1164256629;N:51332 | 101 | 101 | 1166167313 | 1050165932 | 1062683912 | 1164256629 | 51332 | SRX12848199 | SRS10791664 | SRA1320158 | University of Chile|INTA | University of Chile | 2 | 0.96096 | 0.96463 | 0.06551 | 0.06521 | 0.68091 | 0.67884 | 0.46365 | 0.47531 | 101 | 101 | B | B | biological fallback assumption | illumina | novaseq_era | unknown | random_priming | trueseq | bulk | unknown | unknown | Chile | 2021-10-31 | Undetermined | Larval | Trunk | Surface Structure | ||||||||||||||||||||
| 66810 | 66810 | SRR16647490 | SRX12848198 | SRS10791664 | SRP343938 | PRJNA776643 | RNAseq study for assessed the microcystin LR and geosmin differential gene expression modulation in zebrafish larvae 30 dph | PRJNA776643 | Other | Microcystin LR and Geosmin effects over whole body zebrafish Danio rerio larvae transcriptome | Cyanotoxins exposition | Cyanotoxins | strain:Wild|dev stage:31 dph|sex:Not collected|tissue:whole body|BioSampleModel:Model organism or animal | Control Group | Control4 | Control4 | TruSeq stranded mRNA library | RNA-Seq | TRANSCRIPTOMIC | RANDOM | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP343938 | Control_9_1.fastq.gz Control_9_2.fastq.gz | fastq fastq | 4929324190.0 | 24402595.0 | Control 9 1.fastq.gz | 0:101 1:101 | A:1284447529;C:1179260179;G:1182075889;T:1283482794;N:57799 | 101 | 101 | 1284447529 | 1179260179 | 1182075889 | 1283482794 | 57799 | SRX12848198 | SRS10791664 | SRA1320158 | University of Chile|INTA | University of Chile | 2 | 0.96315 | 0.96562 | 0.0579 | 0.05684 | 0.6872 | 0.6854 | 0.4698 | 0.47885 | 101 | 101 | B | B | biological fallback assumption | illumina | novaseq_era | unknown | random_priming | trueseq | bulk | unknown | unknown | Chile | 2021-10-31 | Undetermined | Larval | Trunk | Surface Structure | ||||||||||||||||||||
| 66811 | 66811 | SRR16647491 | SRX12848197 | SRS10791664 | SRP343938 | PRJNA776643 | RNAseq study for assessed the microcystin LR and geosmin differential gene expression modulation in zebrafish larvae 30 dph | PRJNA776643 | Other | Microcystin LR and Geosmin effects over whole body zebrafish Danio rerio larvae transcriptome | Cyanotoxins exposition | Cyanotoxins | strain:Wild|dev stage:31 dph|sex:Not collected|tissue:whole body|BioSampleModel:Model organism or animal | Control group | Control3 | Control3 | TruSeq stranded mRNA library | RNA-Seq | TRANSCRIPTOMIC | RANDOM | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP343938 | Control_7_1.fastq.gz Control_7_2.fastq.gz | fastq fastq | 5451223308.0 | 26986254.0 | Control 7 1.fastq.gz | 0:101 1:101 | A:1430742224;C:1290445675;G:1297973588;T:1431995526;N:66295 | 101 | 101 | 1430742224 | 1290445675 | 1297973588 | 1431995526 | 66295 | SRX12848197 | SRS10791664 | SRA1320158 | University of Chile|INTA | University of Chile | 2 | 0.95748 | 0.96139 | 0.06511 | 0.06325 | 0.6786 | 0.67683 | 0.48371 | 0.47809 | 101 | 101 | B | B | biological fallback assumption | illumina | novaseq_era | unknown | random_priming | trueseq | bulk | unknown | unknown | Chile | 2021-10-31 | Undetermined | Larval | Trunk | Surface Structure | ||||||||||||||||||||
| 66812 | 66812 | SRR16647492 | SRX12848196 | SRS10791664 | SRP343938 | PRJNA776643 | RNAseq study for assessed the microcystin LR and geosmin differential gene expression modulation in zebrafish larvae 30 dph | PRJNA776643 | Other | Microcystin LR and Geosmin effects over whole body zebrafish Danio rerio larvae transcriptome | Cyanotoxins exposition | Cyanotoxins | strain:Wild|dev stage:31 dph|sex:Not collected|tissue:whole body|BioSampleModel:Model organism or animal | MCLR Group | Microcystin5 | Microcystin5 | TruSeq stranded mRNA library | RNA-Seq | TRANSCRIPTOMIC | RANDOM | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP343938 | MCLR_9_2.fastq.gz MCLR_9_1.fastq.gz | fastq fastq | 6319760688.0 | 31285944.0 | MCLR 9 1.fastq.gz | 0:101 1:101 | A:1651386984;C:1506636394;G:1503844710;T:1657818616;N:73984 | 101 | 101 | 1651386984 | 1506636394 | 1503844710 | 1657818616 | 73984 | SRX12848196 | SRS10791664 | SRA1320158 | University of Chile|INTA | University of Chile | 2 | 0.96138 | 0.9653 | 0.06138 | 0.06096 | 0.68527 | 0.68375 | 0.47357 | 0.47623 | 101 | 101 | B | B | biological fallback assumption | illumina | novaseq_era | unknown | random_priming | trueseq | bulk | unknown | unknown | Chile | 2021-10-31 | Undetermined | Larval | Trunk | Surface Structure | ||||||||||||||||||||
| 66813 | 66813 | SRR16647493 | SRX12848195 | SRS10791664 | SRP343938 | PRJNA776643 | RNAseq study for assessed the microcystin LR and geosmin differential gene expression modulation in zebrafish larvae 30 dph | PRJNA776643 | Other | Microcystin LR and Geosmin effects over whole body zebrafish Danio rerio larvae transcriptome | Cyanotoxins exposition | Cyanotoxins | strain:Wild|dev stage:31 dph|sex:Not collected|tissue:whole body|BioSampleModel:Model organism or animal | MCLR Group | Microcystin4 | Microcystin4 | TruSeq stranded mRNA library | RNA-Seq | TRANSCRIPTOMIC | RANDOM | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP343938 | MCLR_6_1.fastq.gz MCLR_6_2.fastq.gz | fastq fastq | 5958169780.0 | 29495890.0 | MCLR 6 1.fastq.gz | 0:101 1:101 | A:1554274821;C:1422499065;G:1422743047;T:1558582611;N:70236 | 101 | 101 | 1554274821 | 1422499065 | 1422743047 | 1558582611 | 70236 | SRX12848195 | SRS10791664 | SRA1320158 | University of Chile|INTA | University of Chile | 2 | 0.96155 | 0.96352 | 0.05329 | 0.05208 | 0.67838 | 0.67671 | 0.46412 | 0.46602 | 101 | 101 | B | B | biological fallback assumption | illumina | novaseq_era | unknown | random_priming | trueseq | bulk | unknown | unknown | Chile | 2021-10-31 | Undetermined | Larval | Trunk | Surface Structure | ||||||||||||||||||||
| 66814 | 66814 | SRR16647494 | SRX12848194 | SRS10791664 | SRP343938 | PRJNA776643 | RNAseq study for assessed the microcystin LR and geosmin differential gene expression modulation in zebrafish larvae 30 dph | PRJNA776643 | Other | Microcystin LR and Geosmin effects over whole body zebrafish Danio rerio larvae transcriptome | Cyanotoxins exposition | Cyanotoxins | strain:Wild|dev stage:31 dph|sex:Not collected|tissue:whole body|BioSampleModel:Model organism or animal | MCLR Group | Microcystin3 | Microcystin3 | TruSeq stranded mRNA library | RNA-Seq | TRANSCRIPTOMIC | RANDOM | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP343938 | MCLR_4_1.fastq.gz MCLR_4_2.fastq.gz | fastq fastq | 4299813410.0 | 21286205.0 | MCLR 4 1.fastq.gz | 0:101 1:101 | A:1130530424;C:1017015892;G:1018283950;T:1133933238;N:49906 | 101 | 101 | 1130530424 | 1017015892 | 1018283950 | 1133933238 | 49906 | SRX12848194 | SRS10791664 | SRA1320158 | University of Chile|INTA | University of Chile | 2 | 0.95994 | 0.96417 | 0.06438 | 0.06329 | 0.68164 | 0.68024 | 0.46908 | 0.45721 | 101 | 101 | B | B | biological fallback assumption | illumina | novaseq_era | unknown | random_priming | trueseq | bulk | unknown | unknown | Chile | 2021-10-31 | Undetermined | Larval | Trunk | Surface Structure | ||||||||||||||||||||
| 66815 | 66815 | SRR16647495 | SRX12848193 | SRS10791664 | SRP343938 | PRJNA776643 | RNAseq study for assessed the microcystin LR and geosmin differential gene expression modulation in zebrafish larvae 30 dph | PRJNA776643 | Other | Microcystin LR and Geosmin effects over whole body zebrafish Danio rerio larvae transcriptome | Cyanotoxins exposition | Cyanotoxins | strain:Wild|dev stage:31 dph|sex:Not collected|tissue:whole body|BioSampleModel:Model organism or animal | MCLR Group | Microcystin2 | Microcystin2 | TruSeq stranded mRNA library | RNA-Seq | TRANSCRIPTOMIC | RANDOM | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP343938 | MCLR_2_1.fastq.gz MCLR_2_2.fastq.gz | fastq fastq | 4423131582.0 | 21896691.0 | MCLR 2 1.fastq.gz | 0:101 1:101 | A:1149883296;C:1057886123;G:1061611354;T:1153698579;N:52230 | 101 | 101 | 1149883296 | 1057886123 | 1061611354 | 1153698579 | 52230 | SRX12848193 | SRS10791664 | SRA1320158 | University of Chile|INTA | University of Chile | 2 | 0.95613 | 0.95907 | 0.05023 | 0.04906 | 0.66797 | 0.66728 | 0.46443 | 0.47031 | 101 | 101 | B | B | biological fallback assumption | illumina | novaseq_era | unknown | random_priming | trueseq | bulk | unknown | unknown | Chile | 2021-10-31 | Undetermined | Larval | Trunk | Surface Structure | ||||||||||||||||||||
| 66816 | 66816 | SRR16647496 | SRX12848192 | SRS10791664 | SRP343938 | PRJNA776643 | RNAseq study for assessed the microcystin LR and geosmin differential gene expression modulation in zebrafish larvae 30 dph | PRJNA776643 | Other | Microcystin LR and Geosmin effects over whole body zebrafish Danio rerio larvae transcriptome | Cyanotoxins exposition | Cyanotoxins | strain:Wild|dev stage:31 dph|sex:Not collected|tissue:whole body|BioSampleModel:Model organism or animal | MCLR Group | Microcystin1 | Microcystin1 | TruSeq stranded mRNA library | RNA-Seq | TRANSCRIPTOMIC | RANDOM | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP343938 | MCLR_1_1.fastq.gz MCLR_1_2.fastq.gz | fastq fastq | 4515806556.0 | 22355478.0 | MCLR 1 1.fastq.gz | 0:101 1:101 | A:1182700971;C:1072987233;G:1076179047;T:1183885831;N:53474 | 101 | 101 | 1182700971 | 1072987233 | 1076179047 | 1183885831 | 53474 | SRX12848192 | SRS10791664 | SRA1320158 | University of Chile|INTA | University of Chile | 2 | 0.96333 | 0.96773 | 0.05947 | 0.05827 | 0.68674 | 0.68592 | 0.48455 | 0.48382 | 101 | 101 | B | B | biological fallback assumption | illumina | novaseq_era | unknown | random_priming | trueseq | bulk | unknown | unknown | Chile | 2021-10-31 | Undetermined | Larval | Trunk | Surface Structure | ||||||||||||||||||||
| 66817 | 66817 | SRR16647497 | SRX12848191 | SRS10791664 | SRP343938 | PRJNA776643 | RNAseq study for assessed the microcystin LR and geosmin differential gene expression modulation in zebrafish larvae 30 dph | PRJNA776643 | Other | Microcystin LR and Geosmin effects over whole body zebrafish Danio rerio larvae transcriptome | Cyanotoxins exposition | Cyanotoxins | strain:Wild|dev stage:31 dph|sex:Not collected|tissue:whole body|BioSampleModel:Model organism or animal | Control group | Control2 | Control2 | TruSeq stranded mRNA library | RNA-Seq | TRANSCRIPTOMIC | RANDOM | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP343938 | Control_4_2.fastq.gz Control_4_1.fastq.gz | fastq fastq | 5751744364.0 | 28473982.0 | Control 4 1.fastq.gz | 0:101 1:101 | A:1506277501;C:1366270345;G:1370493240;T:1508634723;N:68555 | 101 | 101 | 1506277501 | 1366270345 | 1370493240 | 1508634723 | 68555 | SRX12848191 | SRS10791664 | SRA1320158 | University of Chile|INTA | University of Chile | 2 | 0.95986 | 0.96351 | 0.06011 | 0.05885 | 0.66849 | 0.66665 | 0.48168 | 0.48854 | 101 | 101 | B | B | biological fallback assumption | illumina | novaseq_era | unknown | random_priming | trueseq | bulk | unknown | unknown | Chile | 2021-10-31 | Undetermined | Larval | Trunk | Surface Structure | ||||||||||||||||||||
| 66818 | 66818 | SRR16647498 | SRX12848190 | SRS10791664 | SRP343938 | PRJNA776643 | RNAseq study for assessed the microcystin LR and geosmin differential gene expression modulation in zebrafish larvae 30 dph | PRJNA776643 | Other | Microcystin LR and Geosmin effects over whole body zebrafish Danio rerio larvae transcriptome | Cyanotoxins exposition | Cyanotoxins | strain:Wild|dev stage:31 dph|sex:Not collected|tissue:whole body|BioSampleModel:Model organism or animal | Control group | Control1 | Control1 | TruSeq stranded mRNA library | RNA-Seq | TRANSCRIPTOMIC | RANDOM | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP343938 | Control_1_1.fastq.gz Control_1_2.fastq.gz | fastq fastq | 5858177356.0 | 29000878.0 | Control 1 1.fastq.gz | 0:101 1:101 | A:1526389428;C:1399991038;G:1403067225;T:1528661140;N:68525 | 101 | 101 | 1526389428 | 1399991038 | 1403067225 | 1528661140 | 68525 | SRX12848190 | SRS10791664 | SRA1320158 | University of Chile|INTA | University of Chile | 2 | 0.96083 | 0.96528 | 0.05769 | 0.05658 | 0.67393 | 0.67294 | 0.4684 | 0.46759 | 101 | 101 | B | B | biological fallback assumption | illumina | novaseq_era | unknown | random_priming | trueseq | bulk | unknown | unknown | Chile | 2021-10-31 | Undetermined | Larval | Trunk | Surface Structure |
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CREATE TABLE run_metadata("run.accession" VARCHAR, "experiment.accession" VARCHAR, "sample.accession" VARCHAR, "study.accession" VARCHAR, bioproject VARCHAR, "study.title" VARCHAR, "study.alias" VARCHAR, "study.type" VARCHAR, "study.abstract" VARCHAR, "study.attributes" VARCHAR, "study.PMIDs" VARCHAR, "sample.description" VARCHAR, "sample.title" VARCHAR, "sample.alias" VARCHAR, "sample.centername" VARCHAR, "sample.attributes" VARCHAR, "GEOsample.title" VARCHAR, "GEOsample.dataprocessing" VARCHAR, "GEOsample.source" VARCHAR, "GEOsample.treatmentprotocol" VARCHAR, "GEOsample.extractprotocol" VARCHAR, "GEOsample.growthprotocol" VARCHAR, "GEOsample.characteristics" VARCHAR, "GEOsample.accession" VARCHAR, "experiment.title" VARCHAR, "experiment.alias" VARCHAR, "experiment.library_name" VARCHAR, "experiment.design_description" VARCHAR, "experiment.library_construction_protocol" VARCHAR, "experiment.attributes" VARCHAR, "experiment.library_strategy" VARCHAR, "experiment.library_source" VARCHAR, "experiment.library_selection" VARCHAR, "experiment.library_layout" VARCHAR, "experiment.platform" VARCHAR, "experiment.instrument_model" VARCHAR, "experiment.spot_descriptor" VARCHAR, "experiment.study_ref" VARCHAR, "run.title" VARCHAR, "run.attributes" VARCHAR, "run.filename" VARCHAR, "run.semantic_name" VARCHAR, "run.total_bases" DOUBLE, "run.total_spots" DOUBLE, "run.alias" VARCHAR, "run.read_lengths" VARCHAR, "run.base_counts" VARCHAR, "run.r1_length" BIGINT, "run.r2_length" BIGINT, "run.r3_length" BIGINT, "run.r4_length" BIGINT, "run.Acount" BIGINT, "run.Ccount" BIGINT, "run.Gcount" BIGINT, "run.Tcount" BIGINT, "run.Ncount" BIGINT, "run.experiment" VARCHAR, "run.pool_member" VARCHAR, "submission.accession" VARCHAR, "submission.srasource" VARCHAR, "submission.bioprojectsource" VARCHAR, "seqdetective.n_mates" BIGINT, "seqdetective.mapping_rate.mate1" DOUBLE, "seqdetective.mapping_rate.mate2" DOUBLE, "seqdetective.nofeature_rate.mate1" DOUBLE, "seqdetective.nofeature_rate.mate2" DOUBLE, "seqdetective.sparsity.mate1" DOUBLE, "seqdetective.sparsity.mate2" DOUBLE, "seqdetective.pos_strand_rate.mate1" DOUBLE, "seqdetective.pos_strand_rate.mate2" DOUBLE, "seqdetective.readlen.mate1" BIGINT, "seqdetective.readlen.mate2" BIGINT, "seqdetective.judgement.mate1" VARCHAR, "seqdetective.judgement.mate2" VARCHAR, "seqdetective.judgement.reason" VARCHAR, platform_family VARCHAR, instrument_generation VARCHAR, read_bias VARCHAR, selection_class VARCHAR, prep_kit VARCHAR, sc_or_bulk VARCHAR, tech_class VARCHAR, technology VARCHAR, tech_variant VARCHAR, "submission.bioprojectsource.country" VARCHAR, earliest_date DATE, devstage_curation VARCHAR, devstage_curation_coarse VARCHAR, tissue_curation VARCHAR, tissue_curation_coarse VARCHAR);;
CREATE INDEX idx_run_bioproject ON run_metadata(bioproject);;
CREATE INDEX idx_run_run_accession ON run_metadata("run.accession");;