run_metadata
35 rows where experiment.library_selection = "RANDOM" and tissue_curation = "Gut"
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| Link | rowid ▼ | run.accession | experiment.accession | sample.accession | study.accession | bioproject | study.title | study.alias | study.type | study.abstract | study.attributes | study.PMIDs | sample.description | sample.title | sample.alias | sample.centername | sample.attributes | GEOsample.title | GEOsample.dataprocessing | GEOsample.source | GEOsample.treatmentprotocol | GEOsample.extractprotocol | GEOsample.growthprotocol | GEOsample.characteristics | GEOsample.accession | experiment.title | experiment.alias | experiment.library_name | experiment.design_description | experiment.library_construction_protocol | experiment.attributes | experiment.library_strategy | experiment.library_source | experiment.library_selection | experiment.library_layout | experiment.platform | experiment.instrument_model | experiment.spot_descriptor | experiment.study_ref | run.title | run.attributes | run.filename | run.semantic_name | run.total_bases | run.total_spots | run.alias | run.read_lengths | run.base_counts | run.r1_length | run.r2_length | run.r3_length | run.r4_length | run.Acount | run.Ccount | run.Gcount | run.Tcount | run.Ncount | run.experiment | run.pool_member | submission.accession | submission.srasource | submission.bioprojectsource | seqdetective.n_mates | seqdetective.mapping_rate.mate1 | seqdetective.mapping_rate.mate2 | seqdetective.nofeature_rate.mate1 | seqdetective.nofeature_rate.mate2 | seqdetective.sparsity.mate1 | seqdetective.sparsity.mate2 | seqdetective.pos_strand_rate.mate1 | seqdetective.pos_strand_rate.mate2 | seqdetective.readlen.mate1 | seqdetective.readlen.mate2 | seqdetective.judgement.mate1 | seqdetective.judgement.mate2 | seqdetective.judgement.reason | platform_family | instrument_generation | read_bias | selection_class | prep_kit | sc_or_bulk | tech_class | technology | tech_variant | submission.bioprojectsource.country | earliest_date | devstage_curation | devstage_curation_coarse | tissue_curation | tissue_curation_coarse |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 32 | 32 | DRR408242 | DRX393848 | DRS407006 | DRP012035 | PRJDB14274 | Zebrafish Gut RNA seq. | DRP012035 | Transcriptome Analysis | A project to find differential expressed genes between larval and adult zebrafish gut. We dissected guts of wild type AB line and prepared three duplicates for each of larval and adult gut. Libraries for NGS are prepared using illumina TruSeq standard mRNA sample prep kit. | zebrafish wild type AB adult gut replicate 3 | zebrafish adult gut replicate 3 | SAMD00529462 | sample name:zebrafish adult gut replicate 3|biological replicate:adult 3|strain:AB | Illumina HiSeq 1500 sequencing of SAMD00529462 | DRX393848 | AR019 gut 6 adult | 1 | 1 | RNA-Seq | TRANSCRIPTOMIC | RANDOM | SINGLE | ILLUMINA | Illumina HiSeq 1500 | <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>126</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> | DRP012035 | Illumina HiSeq 1500 sequencing of SAMD00529462 | 3546347364.0 | 28145614.0 | DRR408242 | 0:126 1:0 | A:919466631;C:829424335;G:818581155;T:978810217;N:65026 | 126 | 0 | 919466631 | 829424335 | 818581155 | 978810217 | 65026 | DRX393848 | DRS407006 | DRA014885 | NIBB|NIBB core research facilities, National Institute for Basic Biology | University of Hyogo | B | usable mapping rate | illumina | hiseq_era | unknown | random_priming | trueseq | bulk | unknown | unknown | Japan | 2024-09-20 | Adult | Adult | Gut | Digestive System | ||||||||||||||||||||||||||||||
| 33 | 33 | DRR408241 | DRX393847 | DRS407005 | DRP012035 | PRJDB14274 | Zebrafish Gut RNA seq. | DRP012035 | Transcriptome Analysis | A project to find differential expressed genes between larval and adult zebrafish gut. We dissected guts of wild type AB line and prepared three duplicates for each of larval and adult gut. Libraries for NGS are prepared using illumina TruSeq standard mRNA sample prep kit. | zebrafish wild type AB adult gut replicate 2 | zebrafish adult gut replicate 2 | SAMD00529461 | sample name:zebrafish adult gut replicate 2|biological replicate:adult 2|strain:AB | Illumina HiSeq 1500 sequencing of SAMD00529461 | DRX393847 | AR006 gut 4 adult | 1 | 1 | RNA-Seq | TRANSCRIPTOMIC | RANDOM | SINGLE | ILLUMINA | Illumina HiSeq 1500 | <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>126</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> | DRP012035 | Illumina HiSeq 1500 sequencing of SAMD00529461 | 3671973648.0 | 29142648.0 | DRR408241 | 0:126 1:0 | A:942167543;C:859431290;G:852661772;T:1017643011;N:70032 | 126 | 0 | 942167543 | 859431290 | 852661772 | 1017643011 | 70032 | DRX393847 | DRS407005 | DRA014885 | NIBB|NIBB core research facilities, National Institute for Basic Biology | University of Hyogo | B | usable mapping rate | illumina | hiseq_era | unknown | random_priming | trueseq | bulk | unknown | unknown | Japan | 2024-09-20 | Adult | Adult | Gut | Digestive System | ||||||||||||||||||||||||||||||
| 34 | 34 | DRR408240 | DRX393846 | DRS407004 | DRP012035 | PRJDB14274 | Zebrafish Gut RNA seq. | DRP012035 | Transcriptome Analysis | A project to find differential expressed genes between larval and adult zebrafish gut. We dissected guts of wild type AB line and prepared three duplicates for each of larval and adult gut. Libraries for NGS are prepared using illumina TruSeq standard mRNA sample prep kit. | zebrafish wild type AB adult gut replicate 1 | zebrafish adult gut replicate 1 | SAMD00529460 | sample name:zebrafish adult gut replicate 1|biological replicate:adult 1|strain:AB | Illumina HiSeq 1500 sequencing of SAMD00529460 | DRX393846 | AR004 gut 2 adult | 1 | 1 | RNA-Seq | TRANSCRIPTOMIC | RANDOM | SINGLE | ILLUMINA | Illumina HiSeq 1500 | <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>126</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> | DRP012035 | Illumina HiSeq 1500 sequencing of SAMD00529460 | 3480523704.0 | 27623204.0 | DRR408240 | 0:126 1:0 | A:898051986;C:827557593;G:816541244;T:938307607;N:65274 | 126 | 0 | 898051986 | 827557593 | 816541244 | 938307607 | 65274 | DRX393846 | DRS407004 | DRA014885 | NIBB|NIBB core research facilities, National Institute for Basic Biology | University of Hyogo | B | usable mapping rate | illumina | hiseq_era | unknown | random_priming | trueseq | bulk | unknown | unknown | Japan | 2024-09-20 | Adult | Adult | Gut | Digestive System | ||||||||||||||||||||||||||||||
| 35 | 35 | DRR408239 | DRX393845 | DRS407003 | DRP012035 | PRJDB14274 | Zebrafish Gut RNA seq. | DRP012035 | Transcriptome Analysis | A project to find differential expressed genes between larval and adult zebrafish gut. We dissected guts of wild type AB line and prepared three duplicates for each of larval and adult gut. Libraries for NGS are prepared using illumina TruSeq standard mRNA sample prep kit. | zebrafish wild type AB larval gut replicate 3 | zebrafish larval gut replicate 3 | SAMD00529459 | sample name:zebrafish larval gut replicate 3|biological replicate:larval 3|strain:AB | Illumina HiSeq 1500 sequencing of SAMD00529459 | DRX393845 | AR012 gut 5 5 dpf 6 dpf larvae | 1 | 1 | RNA-Seq | TRANSCRIPTOMIC | RANDOM | SINGLE | ILLUMINA | Illumina HiSeq 1500 | <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>126</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> | DRP012035 | Illumina HiSeq 1500 sequencing of SAMD00529459 | 3463982046.0 | 27491921.0 | DRR408239 | 0:126 1:0 | A:842552557;C:849757648;G:837664725;T:933942026;N:65090 | 126 | 0 | 842552557 | 849757648 | 837664725 | 933942026 | 65090 | DRX393845 | DRS407003 | DRA014885 | NIBB|NIBB core research facilities, National Institute for Basic Biology | University of Hyogo | B | usable mapping rate | illumina | hiseq_era | unknown | random_priming | trueseq | bulk | unknown | unknown | Japan | 2024-09-20 | Larval | Larval | Gut | Digestive System | ||||||||||||||||||||||||||||||
| 36 | 36 | DRR408238 | DRX393844 | DRS407002 | DRP012035 | PRJDB14274 | Zebrafish Gut RNA seq. | DRP012035 | Transcriptome Analysis | A project to find differential expressed genes between larval and adult zebrafish gut. We dissected guts of wild type AB line and prepared three duplicates for each of larval and adult gut. Libraries for NGS are prepared using illumina TruSeq standard mRNA sample prep kit. | zebrafish wild type AB larval gut replicate 2 | zebrafish larval gut replicate 2 | SAMD00529458 | sample name:zebrafish larval gut replicate 2|biological replicate:larval 2|strain:AB | Illumina HiSeq 1500 sequencing of SAMD00529458 | DRX393844 | AR005 gut 3 5 dpf 6 dpf larvae | 1 | 1 | RNA-Seq | TRANSCRIPTOMIC | RANDOM | SINGLE | ILLUMINA | Illumina HiSeq 1500 | <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>126</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> | DRP012035 | Illumina HiSeq 1500 sequencing of SAMD00529458 | 3782320416.0 | 30018416.0 | DRR408238 | 0:126 1:0 | A:930337206;C:920645770;G:906559955;T:1024704277;N:73208 | 126 | 0 | 930337206 | 920645770 | 906559955 | 1024704277 | 73208 | DRX393844 | DRS407002 | DRA014885 | NIBB|NIBB core research facilities, National Institute for Basic Biology | University of Hyogo | B | usable mapping rate | illumina | hiseq_era | unknown | random_priming | trueseq | bulk | unknown | unknown | Japan | 2024-09-20 | Larval | Larval | Gut | Digestive System | ||||||||||||||||||||||||||||||
| 37 | 37 | DRR408237 | DRX393843 | DRS407001 | DRP012035 | PRJDB14274 | Zebrafish Gut RNA seq. | DRP012035 | Transcriptome Analysis | A project to find differential expressed genes between larval and adult zebrafish gut. We dissected guts of wild type AB line and prepared three duplicates for each of larval and adult gut. Libraries for NGS are prepared using illumina TruSeq standard mRNA sample prep kit. | zebrafish wild type AB larval gut replicate 1 | zebrafish larval gut replicate 1 | SAMD00529457 | sample name:zebrafish larval gut replicate 1|biological replicate:larval 1|strain:AB | Illumina HiSeq 1500 sequencing of SAMD00529457 | DRX393843 | AR002 gut 1 5 dpf 6 dpf larvae | 1 | 1 | RNA-Seq | TRANSCRIPTOMIC | RANDOM | SINGLE | ILLUMINA | Illumina HiSeq 1500 | <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>126</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> | DRP012035 | Illumina HiSeq 1500 sequencing of SAMD00529457 | 3606885828.0 | 28626078.0 | DRR408237 | 0:126 1:0 | A:879148446;C:885673723;G:870330963;T:971663212;N:69484 | 126 | 0 | 879148446 | 885673723 | 870330963 | 971663212 | 69484 | DRX393843 | DRS407001 | DRA014885 | NIBB|NIBB core research facilities, National Institute for Basic Biology | University of Hyogo | B | usable mapping rate | illumina | hiseq_era | unknown | random_priming | trueseq | bulk | unknown | unknown | Japan | 2024-09-20 | Larval | Larval | Gut | Digestive System | ||||||||||||||||||||||||||||||
| 25135 | 25135 | SRR25649156 | SRX21375305 | SRS18618397 | SRP455253 | PRJNA1005695 | Metagenomic survey of zebrafish from the laboratory and the pet trade | PRJNA1005695 | Other | This study involved sequencing of bulk ribo depleted RNA from intestine kidney and spleen tissues of adult zebrafish reared in the laboratory and the pet trade to identify zebrafish associated microbes in different environments. The goal was to identify viruses and other microbes that infect zebrafish to improve colony health monitoring and highlight pathogens that could be used to study infections of relevance to aquaculture and biomedicine. | 18689X8 | strain:Tubingen|dev stage:adult|collection date:2020 11 05|geo loc name:USA: Salt Lake City UT|sex:female|tissue:intestine|isolation source:lab|replicate:CNTF1|BioSampleModel:Model organism or animal | RNA seq of adult Danio rerio from laboratory and pet trade sources | 18689X8 | 18689X8 | Fish were sacrificed and intestines whole kidney marrows and spleens were harvested for processing. All tissues were homogenized using mechanical lysis. RNA was extracted using the directzol kit Zymo. 1ml of TRIzol was used per intestine and 0.5ml of TRIzol used for individual spleens and kidneys. Following RNA extraction samples were DNAse treated Zymo. RNA ScreenTape Agilent was used to assess quality of RNA samples. Only samples with RIN scores >8 were used for analysis. RNA libraries were prepared by the High Throughput Genomics Shared Resource at the University of Utah with the Illumina TruSeq Stranded Total RNA Library Prep Ribo Zero Gold and sequenced on a Novaseq with using a 150x150 bp sequencing kit to a depth of 25 million reads per sample | RNA-Seq | METATRANSCRIPTOMIC | RANDOM | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP455253 | 18689X8_R1.fastq.gz 18689X8_R2.fastq.gz | fastq fastq | 8163984120.0 | 27033060.0 | 18689X8 R1.fastq.gz | 0:151 1:151 | A:1900143724;C:2183735704;G:2147710753;T:1932229591;N:164348 | 151 | 151 | 1900143724 | 2183735704 | 2147710753 | 1932229591 | 164348 | SRX21375305 | SRS18618397 | SRA1693347 | University of Utah|Quantitative Cell Science | University of Utah | 2 | 0.89288 | 0.89479 | 0.20193 | 0.20116 | 0.79072 | 0.79352 | 0.57246 | 0.56409 | 151 | 151 | B | B | biological fallback assumption | illumina | novaseq_era | unknown | random_priming | trueseq | bulk | bulk | bulk | United States | 2023-08-15 | Adult | Adult | Gut | Digestive System | |||||||||||||||||||||
| 25136 | 25136 | SRR25649157 | SRX21375304 | SRS18618396 | SRP455253 | PRJNA1005695 | Metagenomic survey of zebrafish from the laboratory and the pet trade | PRJNA1005695 | Other | This study involved sequencing of bulk ribo depleted RNA from intestine kidney and spleen tissues of adult zebrafish reared in the laboratory and the pet trade to identify zebrafish associated microbes in different environments. The goal was to identify viruses and other microbes that infect zebrafish to improve colony health monitoring and highlight pathogens that could be used to study infections of relevance to aquaculture and biomedicine. | 19629X15 | strain:unknown|dev stage:adult|collection date:2022 03 15|geo loc name:USA: Salt Lake City UT|sex:female|tissue:intestine|isolation source:pet trade 3|replicate:T3|BioSampleModel:Model organism or animal | RNA seq of adult Danio rerio from laboratory and pet trade sources | 19629X15 | 19629X15 | Fish were sacrificed and intestines whole kidney marrows and spleens were harvested for processing. All tissues were homogenized using mechanical lysis. RNA was extracted using the directzol kit Zymo. 1ml of TRIzol was used per intestine and 0.5ml of TRIzol used for individual spleens and kidneys. Following RNA extraction samples were DNAse treated Zymo. RNA ScreenTape Agilent was used to assess quality of RNA samples. Only samples with RIN scores >8 were used for analysis. RNA libraries were prepared by the High Throughput Genomics Shared Resource at the University of Utah with the Illumina TruSeq Stranded Total RNA Library Prep Ribo Zero Gold and sequenced on a Novaseq with using a 150x150 bp sequencing kit to a depth of 25 million reads per sample | RNA-Seq | METATRANSCRIPTOMIC | RANDOM | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP455253 | 19629X15_R2.fastq.gz 19629X15_R1.fastq.gz | fastq fastq | 10277997106.0 | 34033103.0 | 19629X15 R1.fastq.gz | 0:151 1:151 | A:2798108841;C:2341310321;G:2438634645;T:2699729301;N:213998 | 151 | 151 | 2798108841 | 2341310321 | 2438634645 | 2699729301 | 213998 | SRX21375304 | SRS18618396 | SRA1693347 | University of Utah|Quantitative Cell Science | University of Utah | 2 | 0.90655 | 0.90714 | 0.18082 | 0.18027 | 0.72803 | 0.72953 | 0.45722 | 0.45335 | 151 | 151 | B | B | biological fallback assumption | illumina | novaseq_era | unknown | random_priming | trueseq | bulk | bulk | bulk | United States | 2023-08-15 | Adult | Adult | Gut | Digestive System | |||||||||||||||||||||
| 25137 | 25137 | SRR25649158 | SRX21375303 | SRS18618395 | SRP455253 | PRJNA1005695 | Metagenomic survey of zebrafish from the laboratory and the pet trade | PRJNA1005695 | Other | This study involved sequencing of bulk ribo depleted RNA from intestine kidney and spleen tissues of adult zebrafish reared in the laboratory and the pet trade to identify zebrafish associated microbes in different environments. The goal was to identify viruses and other microbes that infect zebrafish to improve colony health monitoring and highlight pathogens that could be used to study infections of relevance to aquaculture and biomedicine. | 19629X14 | strain:unknown|dev stage:adult|collection date:2022 03 15|geo loc name:USA: Salt Lake City UT|sex:male|tissue:intestine|isolation source:pet trade 3|replicate:T2|BioSampleModel:Model organism or animal | RNA seq of adult Danio rerio from laboratory and pet trade sources | 19629X14 | 19629X14 | Fish were sacrificed and intestines whole kidney marrows and spleens were harvested for processing. All tissues were homogenized using mechanical lysis. RNA was extracted using the directzol kit Zymo. 1ml of TRIzol was used per intestine and 0.5ml of TRIzol used for individual spleens and kidneys. Following RNA extraction samples were DNAse treated Zymo. RNA ScreenTape Agilent was used to assess quality of RNA samples. Only samples with RIN scores >8 were used for analysis. RNA libraries were prepared by the High Throughput Genomics Shared Resource at the University of Utah with the Illumina TruSeq Stranded Total RNA Library Prep Ribo Zero Gold and sequenced on a Novaseq with using a 150x150 bp sequencing kit to a depth of 25 million reads per sample | RNA-Seq | METATRANSCRIPTOMIC | RANDOM | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP455253 | 19629X14_R1.fastq.gz 19629X14_R2.fastq.gz | fastq fastq | 10921707086.0 | 36164593.0 | 19629X14 R1.fastq.gz | 0:151 1:151 | A:2969229092;C:2484790148;G:2595052715;T:2872407360;N:227771 | 151 | 151 | 2969229092 | 2484790148 | 2595052715 | 2872407360 | 227771 | SRX21375303 | SRS18618395 | SRA1693347 | University of Utah|Quantitative Cell Science | University of Utah | 2 | 0.90015 | 0.90211 | 0.19548 | 0.19546 | 0.72997 | 0.73131 | 0.53978 | 0.54998 | 151 | 151 | B | B | biological fallback assumption | illumina | novaseq_era | unknown | random_priming | trueseq | bulk | bulk | bulk | United States | 2023-08-15 | Adult | Adult | Gut | Digestive System | |||||||||||||||||||||
| 25138 | 25138 | SRR25649159 | SRX21375302 | SRS18618394 | SRP455253 | PRJNA1005695 | Metagenomic survey of zebrafish from the laboratory and the pet trade | PRJNA1005695 | Other | This study involved sequencing of bulk ribo depleted RNA from intestine kidney and spleen tissues of adult zebrafish reared in the laboratory and the pet trade to identify zebrafish associated microbes in different environments. The goal was to identify viruses and other microbes that infect zebrafish to improve colony health monitoring and highlight pathogens that could be used to study infections of relevance to aquaculture and biomedicine. | 19629X13 | strain:unknown|dev stage:adult|collection date:2022 03 15|geo loc name:USA: Salt Lake City UT|sex:female|tissue:intestine|isolation source:pet trade 3|replicate:T1|BioSampleModel:Model organism or animal | RNA seq of adult Danio rerio from laboratory and pet trade sources | 19629X13 | 19629X13 | Fish were sacrificed and intestines whole kidney marrows and spleens were harvested for processing. All tissues were homogenized using mechanical lysis. RNA was extracted using the directzol kit Zymo. 1ml of TRIzol was used per intestine and 0.5ml of TRIzol used for individual spleens and kidneys. Following RNA extraction samples were DNAse treated Zymo. RNA ScreenTape Agilent was used to assess quality of RNA samples. Only samples with RIN scores >8 were used for analysis. RNA libraries were prepared by the High Throughput Genomics Shared Resource at the University of Utah with the Illumina TruSeq Stranded Total RNA Library Prep Ribo Zero Gold and sequenced on a Novaseq with using a 150x150 bp sequencing kit to a depth of 25 million reads per sample | RNA-Seq | METATRANSCRIPTOMIC | RANDOM | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP455253 | 19629X13_R1.fastq.gz 19629X13_R2.fastq.gz | fastq fastq | 10019262230.0 | 33176365.0 | 19629X13 R1.fastq.gz | 0:151 1:151 | A:2732766602;C:2270429150;G:2391166434;T:2624690350;N:209694 | 151 | 151 | 2732766602 | 2270429150 | 2391166434 | 2624690350 | 209694 | SRX21375302 | SRS18618394 | SRA1693347 | University of Utah|Quantitative Cell Science | University of Utah | 2 | 0.90381 | 0.90536 | 0.18425 | 0.18388 | 0.72681 | 0.72936 | 0.48627 | 0.49398 | 151 | 151 | B | B | biological fallback assumption | illumina | novaseq_era | unknown | random_priming | trueseq | bulk | bulk | bulk | United States | 2023-08-15 | Adult | Adult | Gut | Digestive System | |||||||||||||||||||||
| 25139 | 25139 | SRR25649160 | SRX21375301 | SRS18618393 | SRP455253 | PRJNA1005695 | Metagenomic survey of zebrafish from the laboratory and the pet trade | PRJNA1005695 | Other | This study involved sequencing of bulk ribo depleted RNA from intestine kidney and spleen tissues of adult zebrafish reared in the laboratory and the pet trade to identify zebrafish associated microbes in different environments. The goal was to identify viruses and other microbes that infect zebrafish to improve colony health monitoring and highlight pathogens that could be used to study infections of relevance to aquaculture and biomedicine. | 19629X12 | strain:unknown|dev stage:adult|collection date:2022 03 15|geo loc name:USA: Salt Lake City UT|sex:female|tissue:intestine|isolation source:pet trade 2|replicate:E3|BioSampleModel:Model organism or animal | RNA seq of adult Danio rerio from laboratory and pet trade sources | 19629X12 | 19629X12 | Fish were sacrificed and intestines whole kidney marrows and spleens were harvested for processing. All tissues were homogenized using mechanical lysis. RNA was extracted using the directzol kit Zymo. 1ml of TRIzol was used per intestine and 0.5ml of TRIzol used for individual spleens and kidneys. Following RNA extraction samples were DNAse treated Zymo. RNA ScreenTape Agilent was used to assess quality of RNA samples. Only samples with RIN scores >8 were used for analysis. RNA libraries were prepared by the High Throughput Genomics Shared Resource at the University of Utah with the Illumina TruSeq Stranded Total RNA Library Prep Ribo Zero Gold and sequenced on a Novaseq with using a 150x150 bp sequencing kit to a depth of 25 million reads per sample | RNA-Seq | METATRANSCRIPTOMIC | RANDOM | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP455253 | 19629X12_R1.fastq.gz 19629X12_R2.fastq.gz | fastq fastq | 11078632326.0 | 36684213.0 | 19629X12 R1.fastq.gz | 0:151 1:151 | A:2995473302;C:2550446088;G:2653594689;T:2878887522;N:230725 | 151 | 151 | 2995473302 | 2550446088 | 2653594689 | 2878887522 | 230725 | SRX21375301 | SRS18618393 | SRA1693347 | University of Utah|Quantitative Cell Science | University of Utah | 2 | 0.91101 | 0.91121 | 0.16145 | 0.16142 | 0.70274 | 0.70498 | 0.47852 | 0.48788 | 151 | 151 | B | B | biological fallback assumption | illumina | novaseq_era | unknown | random_priming | trueseq | bulk | bulk | bulk | United States | 2023-08-15 | Adult | Adult | Gut | Digestive System | |||||||||||||||||||||
| 25140 | 25140 | SRR25649161 | SRX21375300 | SRS18618392 | SRP455253 | PRJNA1005695 | Metagenomic survey of zebrafish from the laboratory and the pet trade | PRJNA1005695 | Other | This study involved sequencing of bulk ribo depleted RNA from intestine kidney and spleen tissues of adult zebrafish reared in the laboratory and the pet trade to identify zebrafish associated microbes in different environments. The goal was to identify viruses and other microbes that infect zebrafish to improve colony health monitoring and highlight pathogens that could be used to study infections of relevance to aquaculture and biomedicine. | 19629X11 | strain:unknown|dev stage:adult|collection date:2022 03 15|geo loc name:USA: Salt Lake City UT|sex:female|tissue:intestine|isolation source:pet trade 2|replicate:E2|BioSampleModel:Model organism or animal | RNA seq of adult Danio rerio from laboratory and pet trade sources | 19629X11 | 19629X11 | Fish were sacrificed and intestines whole kidney marrows and spleens were harvested for processing. All tissues were homogenized using mechanical lysis. RNA was extracted using the directzol kit Zymo. 1ml of TRIzol was used per intestine and 0.5ml of TRIzol used for individual spleens and kidneys. Following RNA extraction samples were DNAse treated Zymo. RNA ScreenTape Agilent was used to assess quality of RNA samples. Only samples with RIN scores >8 were used for analysis. RNA libraries were prepared by the High Throughput Genomics Shared Resource at the University of Utah with the Illumina TruSeq Stranded Total RNA Library Prep Ribo Zero Gold and sequenced on a Novaseq with using a 150x150 bp sequencing kit to a depth of 25 million reads per sample | RNA-Seq | METATRANSCRIPTOMIC | RANDOM | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP455253 | 19629X11_R1.fastq.gz 19629X11_R2.fastq.gz | fastq fastq | 10957183630.0 | 36282065.0 | 19629X11 R1.fastq.gz | 0:151 1:151 | A:2987773085;C:2485587980;G:2603747193;T:2879845340;N:230032 | 151 | 151 | 2987773085 | 2485587980 | 2603747193 | 2879845340 | 230032 | SRX21375300 | SRS18618392 | SRA1693347 | University of Utah|Quantitative Cell Science | University of Utah | 2 | 0.91198 | 0.91301 | 0.20992 | 0.20926 | 0.73044 | 0.73316 | 0.44301 | 0.43596 | 151 | 151 | B | B | biological fallback assumption | illumina | novaseq_era | unknown | random_priming | trueseq | bulk | bulk | bulk | United States | 2023-08-15 | Adult | Adult | Gut | Digestive System | |||||||||||||||||||||
| 25141 | 25141 | SRR25649162 | SRX21375299 | SRS18618390 | SRP455253 | PRJNA1005695 | Metagenomic survey of zebrafish from the laboratory and the pet trade | PRJNA1005695 | Other | This study involved sequencing of bulk ribo depleted RNA from intestine kidney and spleen tissues of adult zebrafish reared in the laboratory and the pet trade to identify zebrafish associated microbes in different environments. The goal was to identify viruses and other microbes that infect zebrafish to improve colony health monitoring and highlight pathogens that could be used to study infections of relevance to aquaculture and biomedicine. | 19629X10 | strain:unknown|dev stage:adult|collection date:2022 03 15|geo loc name:USA: Salt Lake City UT|sex:female|tissue:intestine|isolation source:pet trade 2|replicate:E1|BioSampleModel:Model organism or animal | RNA seq of adult Danio rerio from laboratory and pet trade sources | 19629X10 | 19629X10 | Fish were sacrificed and intestines whole kidney marrows and spleens were harvested for processing. All tissues were homogenized using mechanical lysis. RNA was extracted using the directzol kit Zymo. 1ml of TRIzol was used per intestine and 0.5ml of TRIzol used for individual spleens and kidneys. Following RNA extraction samples were DNAse treated Zymo. RNA ScreenTape Agilent was used to assess quality of RNA samples. Only samples with RIN scores >8 were used for analysis. RNA libraries were prepared by the High Throughput Genomics Shared Resource at the University of Utah with the Illumina TruSeq Stranded Total RNA Library Prep Ribo Zero Gold and sequenced on a Novaseq with using a 150x150 bp sequencing kit to a depth of 25 million reads per sample | RNA-Seq | METATRANSCRIPTOMIC | RANDOM | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP455253 | 19629X10_R1.fastq.gz 19629X10_R2.fastq.gz | fastq fastq | 9549065142.0 | 31619421.0 | 19629X10 R1.fastq.gz | 0:151 1:151 | A:2603092155;C:2167963952;G:2264536965;T:2513273275;N:198795 | 151 | 151 | 2603092155 | 2167963952 | 2264536965 | 2513273275 | 198795 | SRX21375299 | SRS18618390 | SRA1693347 | University of Utah|Quantitative Cell Science | University of Utah | 2 | 0.92549 | 0.92615 | 0.16176 | 0.15925 | 0.7683 | 0.76926 | 0.3093 | 0.30927 | 151 | 151 | B | B | biological fallback assumption | illumina | novaseq_era | unknown | random_priming | trueseq | bulk | bulk | bulk | United States | 2023-08-15 | Adult | Adult | Gut | Digestive System | |||||||||||||||||||||
| 25144 | 25144 | SRR25649165 | SRX21375296 | SRS18618388 | SRP455253 | PRJNA1005695 | Metagenomic survey of zebrafish from the laboratory and the pet trade | PRJNA1005695 | Other | This study involved sequencing of bulk ribo depleted RNA from intestine kidney and spleen tissues of adult zebrafish reared in the laboratory and the pet trade to identify zebrafish associated microbes in different environments. The goal was to identify viruses and other microbes that infect zebrafish to improve colony health monitoring and highlight pathogens that could be used to study infections of relevance to aquaculture and biomedicine. | 18843X6 | strain:Tubingen|dev stage:adult|collection date:2020 11 05|geo loc name:USA: Salt Lake City UT|sex:male|tissue:intestine|isolation source:lab cohoused|replicate:CHM3|BioSampleModel:Model organism or animal | RNA seq of adult Danio rerio from laboratory and pet trade sources | 18843X6 | 18843X6 | Fish were sacrificed and intestines whole kidney marrows and spleens were harvested for processing. All tissues were homogenized using mechanical lysis. RNA was extracted using the directzol kit Zymo. 1ml of TRIzol was used per intestine and 0.5ml of TRIzol used for individual spleens and kidneys. Following RNA extraction samples were DNAse treated Zymo. RNA ScreenTape Agilent was used to assess quality of RNA samples. Only samples with RIN scores >8 were used for analysis. RNA libraries were prepared by the High Throughput Genomics Shared Resource at the University of Utah with the Illumina TruSeq Stranded Total RNA Library Prep Ribo Zero Gold and sequenced on a Novaseq with using a 150x150 bp sequencing kit to a depth of 25 million reads per sample | RNA-Seq | METATRANSCRIPTOMIC | RANDOM | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP455253 | 18843X6_R1.fastq.gz 18843X6_R2.fastq.gz | fastq fastq | 8690806510.0 | 28777505.0 | 18843X6 R1.fastq.gz | 0:151 1:151 | A:2346658651;C:2002583855;G:2108507319;T:2232918222;N:138463 | 151 | 151 | 2346658651 | 2002583855 | 2108507319 | 2232918222 | 138463 | SRX21375296 | SRS18618388 | SRA1693347 | University of Utah|Quantitative Cell Science | University of Utah | 2 | 0.91741 | 0.91785 | 0.21796 | 0.21778 | 0.6971 | 0.70102 | 0.49123 | 0.51016 | 151 | 151 | B | B | biological fallback assumption | illumina | novaseq_era | unknown | random_priming | trueseq | bulk | bulk | bulk | United States | 2023-08-15 | Adult | Adult | Gut | Digestive System | |||||||||||||||||||||
| 25147 | 25147 | SRR25649168 | SRX21375293 | SRS18618385 | SRP455253 | PRJNA1005695 | Metagenomic survey of zebrafish from the laboratory and the pet trade | PRJNA1005695 | Other | This study involved sequencing of bulk ribo depleted RNA from intestine kidney and spleen tissues of adult zebrafish reared in the laboratory and the pet trade to identify zebrafish associated microbes in different environments. The goal was to identify viruses and other microbes that infect zebrafish to improve colony health monitoring and highlight pathogens that could be used to study infections of relevance to aquaculture and biomedicine. | 18843X3 | strain:Tubingen|dev stage:adult|collection date:2020 11 05|geo loc name:USA: Salt Lake City UT|sex:male|tissue:intestine|isolation source:lab cohoused|replicate:CHM2|BioSampleModel:Model organism or animal | RNA seq of adult Danio rerio from laboratory and pet trade sources | 18843X3 | 18843X3 | Fish were sacrificed and intestines whole kidney marrows and spleens were harvested for processing. All tissues were homogenized using mechanical lysis. RNA was extracted using the directzol kit Zymo. 1ml of TRIzol was used per intestine and 0.5ml of TRIzol used for individual spleens and kidneys. Following RNA extraction samples were DNAse treated Zymo. RNA ScreenTape Agilent was used to assess quality of RNA samples. Only samples with RIN scores >8 were used for analysis. RNA libraries were prepared by the High Throughput Genomics Shared Resource at the University of Utah with the Illumina TruSeq Stranded Total RNA Library Prep Ribo Zero Gold and sequenced on a Novaseq with using a 150x150 bp sequencing kit to a depth of 25 million reads per sample | RNA-Seq | METATRANSCRIPTOMIC | RANDOM | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP455253 | 18843X3_R1.fastq.gz 18843X3_R2.fastq.gz | fastq fastq | 9351018676.0 | 30963638.0 | 18843X3 R1.fastq.gz | 0:151 1:151 | A:2555870224;C:2133006230;G:2246501165;T:2415491618;N:149439 | 151 | 151 | 2555870224 | 2133006230 | 2246501165 | 2415491618 | 149439 | SRX21375293 | SRS18618385 | SRA1693347 | University of Utah|Quantitative Cell Science | University of Utah | 2 | 0.91594 | 0.91512 | 0.21678 | 0.21621 | 0.72719 | 0.73117 | 0.52961 | 0.53327 | 151 | 151 | B | B | biological fallback assumption | illumina | novaseq_era | unknown | random_priming | trueseq | bulk | bulk | bulk | United States | 2023-08-15 | Adult | Adult | Gut | Digestive System | |||||||||||||||||||||
| 25149 | 25149 | SRR25649170 | SRX21375291 | SRS18618383 | SRP455253 | PRJNA1005695 | Metagenomic survey of zebrafish from the laboratory and the pet trade | PRJNA1005695 | Other | This study involved sequencing of bulk ribo depleted RNA from intestine kidney and spleen tissues of adult zebrafish reared in the laboratory and the pet trade to identify zebrafish associated microbes in different environments. The goal was to identify viruses and other microbes that infect zebrafish to improve colony health monitoring and highlight pathogens that could be used to study infections of relevance to aquaculture and biomedicine. | 19629X9 | strain:unknown|dev stage:adult|collection date:2022 03 15|geo loc name:USA: Salt Lake City UT|sex:male|tissue:intestine|isolation source:pet trade 1|replicate:P3|BioSampleModel:Model organism or animal | RNA seq of adult Danio rerio from laboratory and pet trade sources | 19629X9 | 19629X9 | Fish were sacrificed and intestines whole kidney marrows and spleens were harvested for processing. All tissues were homogenized using mechanical lysis. RNA was extracted using the directzol kit Zymo. 1ml of TRIzol was used per intestine and 0.5ml of TRIzol used for individual spleens and kidneys. Following RNA extraction samples were DNAse treated Zymo. RNA ScreenTape Agilent was used to assess quality of RNA samples. Only samples with RIN scores >8 were used for analysis. RNA libraries were prepared by the High Throughput Genomics Shared Resource at the University of Utah with the Illumina TruSeq Stranded Total RNA Library Prep Ribo Zero Gold and sequenced on a Novaseq with using a 150x150 bp sequencing kit to a depth of 25 million reads per sample | RNA-Seq | METATRANSCRIPTOMIC | RANDOM | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP455253 | 19629X9_R1.fastq.gz 19629X9_R2.fastq.gz | fastq fastq | 12342730940.0 | 40869970.0 | 19629X9 R1.fastq.gz | 0:151 1:151 | A:3339874828;C:2828928610;G:2945452577;T:3228218292;N:256633 | 151 | 151 | 3339874828 | 2828928610 | 2945452577 | 3228218292 | 256633 | SRX21375291 | SRS18618383 | SRA1693347 | University of Utah|Quantitative Cell Science | University of Utah | 2 | 0.90738 | 0.8127 | 0.19825 | 0.17409 | 0.73385 | 0.74659 | 0.54306 | 0.55304 | 151 | 151 | B | B | biological fallback assumption | illumina | novaseq_era | unknown | random_priming | trueseq | bulk | bulk | bulk | United States | 2023-08-15 | Adult | Adult | Gut | Digestive System | |||||||||||||||||||||
| 25151 | 25151 | SRR25649172 | SRX21375289 | SRS18618381 | SRP455253 | PRJNA1005695 | Metagenomic survey of zebrafish from the laboratory and the pet trade | PRJNA1005695 | Other | This study involved sequencing of bulk ribo depleted RNA from intestine kidney and spleen tissues of adult zebrafish reared in the laboratory and the pet trade to identify zebrafish associated microbes in different environments. The goal was to identify viruses and other microbes that infect zebrafish to improve colony health monitoring and highlight pathogens that could be used to study infections of relevance to aquaculture and biomedicine. | 18689X5 | strain:Tubingen|dev stage:adult|collection date:2020 11 05|geo loc name:USA: Salt Lake City UT|sex:male|tissue:intestine|isolation source:lab cohoused|replicate:CHM1|BioSampleModel:Model organism or animal | RNA seq of adult Danio rerio from laboratory and pet trade sources | 18689X5 | 18689X5 | Fish were sacrificed and intestines whole kidney marrows and spleens were harvested for processing. All tissues were homogenized using mechanical lysis. RNA was extracted using the directzol kit Zymo. 1ml of TRIzol was used per intestine and 0.5ml of TRIzol used for individual spleens and kidneys. Following RNA extraction samples were DNAse treated Zymo. RNA ScreenTape Agilent was used to assess quality of RNA samples. Only samples with RIN scores >8 were used for analysis. RNA libraries were prepared by the High Throughput Genomics Shared Resource at the University of Utah with the Illumina TruSeq Stranded Total RNA Library Prep Ribo Zero Gold and sequenced on a Novaseq with using a 150x150 bp sequencing kit to a depth of 25 million reads per sample | RNA-Seq | METATRANSCRIPTOMIC | RANDOM | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP455253 | 18689X5_R1.fastq.gz 18689X5_R2.fastq.gz | fastq fastq | 8136028886.0 | 26940493.0 | 18689X5 R1.fastq.gz | 0:151 1:151 | A:2015403368;C:2051480513;G:2023331135;T:2045661742;N:152128 | 151 | 151 | 2015403368 | 2051480513 | 2023331135 | 2045661742 | 152128 | SRX21375289 | SRS18618381 | SRA1693347 | University of Utah|Quantitative Cell Science | University of Utah | 2 | 0.88067 | 0.88216 | 0.24575 | 0.2425 | 0.75018 | 0.75073 | 0.59361 | 0.61451 | 151 | 151 | B | B | biological fallback assumption | illumina | novaseq_era | unknown | random_priming | trueseq | bulk | bulk | bulk | United States | 2023-08-15 | Adult | Adult | Gut | Digestive System | |||||||||||||||||||||
| 25154 | 25154 | SRR25649175 | SRX21375286 | SRS18618378 | SRP455253 | PRJNA1005695 | Metagenomic survey of zebrafish from the laboratory and the pet trade | PRJNA1005695 | Other | This study involved sequencing of bulk ribo depleted RNA from intestine kidney and spleen tissues of adult zebrafish reared in the laboratory and the pet trade to identify zebrafish associated microbes in different environments. The goal was to identify viruses and other microbes that infect zebrafish to improve colony health monitoring and highlight pathogens that could be used to study infections of relevance to aquaculture and biomedicine. | 18843X9 | strain:Tubingen|dev stage:adult|collection date:2020 11 05|geo loc name:USA: Salt Lake City UT|sex:male|tissue:intestine|isolation source:lab|replicate:CNTM3|BioSampleModel:Model organism or animal | RNA seq of adult Danio rerio from laboratory and pet trade sources | 18843X9 | 18843X9 | Fish were sacrificed and intestines whole kidney marrows and spleens were harvested for processing. All tissues were homogenized using mechanical lysis. RNA was extracted using the directzol kit Zymo. 1ml of TRIzol was used per intestine and 0.5ml of TRIzol used for individual spleens and kidneys. Following RNA extraction samples were DNAse treated Zymo. RNA ScreenTape Agilent was used to assess quality of RNA samples. Only samples with RIN scores >8 were used for analysis. RNA libraries were prepared by the High Throughput Genomics Shared Resource at the University of Utah with the Illumina TruSeq Stranded Total RNA Library Prep Ribo Zero Gold and sequenced on a Novaseq with using a 150x150 bp sequencing kit to a depth of 25 million reads per sample | RNA-Seq | METATRANSCRIPTOMIC | RANDOM | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP455253 | 18843X9_R1.fastq.gz 18843X9_R2.fastq.gz | fastq fastq | 8819654206.0 | 29204153.0 | 18843X9 R1.fastq.gz | 0:151 1:151 | A:2383706177;C:2044504407;G:2140881818;T:2250422256;N:139548 | 151 | 151 | 2383706177 | 2044504407 | 2140881818 | 2250422256 | 139548 | SRX21375286 | SRS18618378 | SRA1693347 | University of Utah|Quantitative Cell Science | University of Utah | 2 | 0.90385 | 0.90437 | 0.21274 | 0.21235 | 0.72604 | 0.73164 | 0.52888 | 0.52909 | 151 | 151 | B | B | biological fallback assumption | illumina | novaseq_era | unknown | random_priming | trueseq | bulk | bulk | bulk | United States | 2023-08-15 | Adult | Adult | Gut | Digestive System | |||||||||||||||||||||
| 25157 | 25157 | SRR25649178 | SRX21375283 | SRS18618375 | SRP455253 | PRJNA1005695 | Metagenomic survey of zebrafish from the laboratory and the pet trade | PRJNA1005695 | Other | This study involved sequencing of bulk ribo depleted RNA from intestine kidney and spleen tissues of adult zebrafish reared in the laboratory and the pet trade to identify zebrafish associated microbes in different environments. The goal was to identify viruses and other microbes that infect zebrafish to improve colony health monitoring and highlight pathogens that could be used to study infections of relevance to aquaculture and biomedicine. | 18689X7 | strain:Tubingen|dev stage:adult|collection date:2020 11 05|geo loc name:USA: Salt Lake City UT|sex:male|tissue:intestine|isolation source:lab|replicate:CNTM2|BioSampleModel:Model organism or animal | RNA seq of adult Danio rerio from laboratory and pet trade sources | 18689X7 | 18689X7 | Fish were sacrificed and intestines whole kidney marrows and spleens were harvested for processing. All tissues were homogenized using mechanical lysis. RNA was extracted using the directzol kit Zymo. 1ml of TRIzol was used per intestine and 0.5ml of TRIzol used for individual spleens and kidneys. Following RNA extraction samples were DNAse treated Zymo. RNA ScreenTape Agilent was used to assess quality of RNA samples. Only samples with RIN scores >8 were used for analysis. RNA libraries were prepared by the High Throughput Genomics Shared Resource at the University of Utah with the Illumina TruSeq Stranded Total RNA Library Prep Ribo Zero Gold and sequenced on a Novaseq with using a 150x150 bp sequencing kit to a depth of 25 million reads per sample | RNA-Seq | METATRANSCRIPTOMIC | RANDOM | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP455253 | 18689X7_R1.fastq.gz 18689X7_R2.fastq.gz | fastq fastq | 8986035368.0 | 29755084.0 | 18689X7 R1.fastq.gz | 0:151 1:151 | A:2169723143;C:2326462981;G:2292317481;T:2197350043;N:181720 | 151 | 151 | 2169723143 | 2326462981 | 2292317481 | 2197350043 | 181720 | SRX21375283 | SRS18618375 | SRA1693347 | University of Utah|Quantitative Cell Science | University of Utah | 2 | 0.89254 | 0.89285 | 0.22562 | 0.22356 | 0.74911 | 0.75089 | 0.6409 | 0.65585 | 151 | 151 | B | B | biological fallback assumption | illumina | novaseq_era | unknown | random_priming | trueseq | bulk | bulk | bulk | United States | 2023-08-15 | Adult | Adult | Gut | Digestive System | |||||||||||||||||||||
| 25160 | 25160 | SRR25649181 | SRX21375280 | SRS18618372 | SRP455253 | PRJNA1005695 | Metagenomic survey of zebrafish from the laboratory and the pet trade | PRJNA1005695 | Other | This study involved sequencing of bulk ribo depleted RNA from intestine kidney and spleen tissues of adult zebrafish reared in the laboratory and the pet trade to identify zebrafish associated microbes in different environments. The goal was to identify viruses and other microbes that infect zebrafish to improve colony health monitoring and highlight pathogens that could be used to study infections of relevance to aquaculture and biomedicine. | 19629X8 | strain:unknown|dev stage:adult|collection date:2022 03 15|geo loc name:USA: Salt Lake City UT|sex:male|tissue:intestine|isolation source:pet trade 1|replicate:P2|BioSampleModel:Model organism or animal | RNA seq of adult Danio rerio from laboratory and pet trade sources | 19629X8 | 19629X8 | Fish were sacrificed and intestines whole kidney marrows and spleens were harvested for processing. All tissues were homogenized using mechanical lysis. RNA was extracted using the directzol kit Zymo. 1ml of TRIzol was used per intestine and 0.5ml of TRIzol used for individual spleens and kidneys. Following RNA extraction samples were DNAse treated Zymo. RNA ScreenTape Agilent was used to assess quality of RNA samples. Only samples with RIN scores >8 were used for analysis. RNA libraries were prepared by the High Throughput Genomics Shared Resource at the University of Utah with the Illumina TruSeq Stranded Total RNA Library Prep Ribo Zero Gold and sequenced on a Novaseq with using a 150x150 bp sequencing kit to a depth of 25 million reads per sample | RNA-Seq | METATRANSCRIPTOMIC | RANDOM | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP455253 | 19629X8_R2.fastq.gz 19629X8_R1.fastq.gz | fastq fastq | 10758135430.0 | 35622965.0 | 19629X8 R1.fastq.gz | 0:151 1:151 | A:2991372806;C:2398155523;G:2517276916;T:2851104238;N:225947 | 151 | 151 | 2991372806 | 2398155523 | 2517276916 | 2851104238 | 225947 | SRX21375280 | SRS18618372 | SRA1693347 | University of Utah|Quantitative Cell Science | University of Utah | 2 | 0.88839 | 0.89055 | 0.23357 | 0.23344 | 0.72592 | 0.72851 | 0.53343 | 0.53081 | 151 | 151 | B | B | biological fallback assumption | illumina | novaseq_era | unknown | random_priming | trueseq | bulk | bulk | bulk | United States | 2023-08-15 | Adult | Adult | Gut | Digestive System | |||||||||||||||||||||
| 25161 | 25161 | SRR25649182 | SRX21375279 | SRS18618371 | SRP455253 | PRJNA1005695 | Metagenomic survey of zebrafish from the laboratory and the pet trade | PRJNA1005695 | Other | This study involved sequencing of bulk ribo depleted RNA from intestine kidney and spleen tissues of adult zebrafish reared in the laboratory and the pet trade to identify zebrafish associated microbes in different environments. The goal was to identify viruses and other microbes that infect zebrafish to improve colony health monitoring and highlight pathogens that could be used to study infections of relevance to aquaculture and biomedicine. | 19629X7 | strain:unknown|dev stage:adult|collection date:2022 03 15|geo loc name:USA: Salt Lake City UT|sex:male|tissue:intestine|isolation source:pet trade 1|replicate:P1|BioSampleModel:Model organism or animal | RNA seq of adult Danio rerio from laboratory and pet trade sources | 19629X7 | 19629X7 | Fish were sacrificed and intestines whole kidney marrows and spleens were harvested for processing. All tissues were homogenized using mechanical lysis. RNA was extracted using the directzol kit Zymo. 1ml of TRIzol was used per intestine and 0.5ml of TRIzol used for individual spleens and kidneys. Following RNA extraction samples were DNAse treated Zymo. RNA ScreenTape Agilent was used to assess quality of RNA samples. Only samples with RIN scores >8 were used for analysis. RNA libraries were prepared by the High Throughput Genomics Shared Resource at the University of Utah with the Illumina TruSeq Stranded Total RNA Library Prep Ribo Zero Gold and sequenced on a Novaseq with using a 150x150 bp sequencing kit to a depth of 25 million reads per sample | RNA-Seq | METATRANSCRIPTOMIC | RANDOM | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP455253 | 19629X7_R1.fastq.gz 19629X7_R2.fastq.gz | fastq fastq | 11738178884.0 | 38868142.0 | 19629X7 R1.fastq.gz | 0:151 1:151 | A:3192358555;C:2674045852;G:2784070515;T:3087458659;N:245303 | 151 | 151 | 3192358555 | 2674045852 | 2784070515 | 3087458659 | 245303 | SRX21375279 | SRS18618371 | SRA1693347 | University of Utah|Quantitative Cell Science | University of Utah | 2 | 0.9074 | 0.90829 | 0.20853 | 0.20916 | 0.74115 | 0.74369 | 0.53704 | 0.55385 | 151 | 151 | B | B | biological fallback assumption | illumina | novaseq_era | unknown | random_priming | trueseq | bulk | bulk | bulk | United States | 2023-08-15 | Adult | Adult | Gut | Digestive System | |||||||||||||||||||||
| 61693 | 61693 | SRR12964273 | SRX9416878 | SRS7632325 | SRP290781 | PRJNA673693 | Global studies of soybean meal dietary in fish | PRJNA673693 | Other | Intestinal transcriptome profile to evaluate dietary soybean meal tolerance favoring growth in adult zebrafish | LG | strain:family generated from TAB 5 DB 3 or AB|isolate:null|breed:zebrafish|age:98 dpf stage:adult|sex:male|tissue:intestine|biomaterial provider:Danio Biotech Company from Zebrafish for Innovation and Research ZIRLab Faculty of Sciences at University of Chile|genotype:low w8 post a soy diet|BioSampleModel:Model organism or animal | RNA Seq of zebrafish adult soy diet | 1 8 R1 | 1 8 R1 | RNA Seq libraries were constructed using TruSeq Stranded mRNA LT Sample Prep Kit following TruSeq Stranded mRNA Sample Preparation Guide Part #15031047 Rev. E. Briefly each sequencing library was prepared by random fragmentation of the DNA or cDNA sample followed by five prime and three prime adapter ligation. Alternatively "tagmentation" combines the fragmentation and ligation reactions into a single step that greatly increases the efficiency of the library preparation process. Adapter ligated fragments were then PCR amplified and gel purified. The fragments were sequenced with a read length of 151 base pairs using Illumina sequencing technology | RNA-Seq | TRANSCRIPTOMIC | RANDOM | PAIRED | ILLUMINA | Illumina HiSeq 1000 | SRP290781 | F1-8-CH_1.fastq.gz F1-8-CH_2.fastq.gz | fastq fastq | 7325280894.0 | 24255897.0 | F1 8 CH 1.fastq.gz | 0:151 1:151 | A:1918036349;C:1737589923;G:1816977467;T:1852649336;N:27819 | 151 | 151 | 1918036349 | 1737589923 | 1816977467 | 1852649336 | 27819 | SRX9416878 | SRS7632325 | SRA1152184 | Universidad de las Americas|Facultad de Medicina Veterinaria y Agronomia | Universidad de las Americas | 2 | 0.95509 | 0.95658 | 0.04684 | 0.04664 | 0.82221 | 0.82059 | 0.60005 | 0.56895 | 151 | 151 | B | B | biological fallback assumption | illumina | hiseq_era | unknown | random_priming | trueseq | bulk | unknown | unknown | Unknown | 2020-11-02 | Adult | Adult | Gut | Digestive System | |||||||||||||||||||||
| 61694 | 61694 | SRR12964274 | SRX9416877 | SRS7632325 | SRP290781 | PRJNA673693 | Global studies of soybean meal dietary in fish | PRJNA673693 | Other | Intestinal transcriptome profile to evaluate dietary soybean meal tolerance favoring growth in adult zebrafish | LG | strain:family generated from TAB 5 DB 3 or AB|isolate:null|breed:zebrafish|age:98 dpf stage:adult|sex:male|tissue:intestine|biomaterial provider:Danio Biotech Company from Zebrafish for Innovation and Research ZIRLab Faculty of Sciences at University of Chile|genotype:low w8 post a soy diet|BioSampleModel:Model organism or animal | RNA Seq of zebrafish adult soy diet | 13 1 R1 | 13 1 R1 | RNA Seq libraries were constructed using TruSeq Stranded mRNA LT Sample Prep Kit following TruSeq Stranded mRNA Sample Preparation Guide Part #15031047 Rev. E. Briefly each sequencing library was prepared by random fragmentation of the DNA or cDNA sample followed by five prime and three prime adapter ligation. Alternatively "tagmentation" combines the fragmentation and ligation reactions into a single step that greatly increases the efficiency of the library preparation process. Adapter ligated fragments were then PCR amplified and gel purified. The fragments were sequenced with a read length of 151 base pairs using Illumina sequencing technology | RNA-Seq | TRANSCRIPTOMIC | RANDOM | PAIRED | ILLUMINA | Illumina HiSeq 1000 | SRP290781 | F13-1-CH_1.fastq.gz F13-1-CH_2.fastq.gz | fastq fastq | 7204681422.0 | 23856561.0 | F13 1 CH 1.fastq.gz | 0:151 1:151 | A:1865960439;C:1728161422;G:1822471558;T:1788060072;N:27931 | 151 | 151 | 1865960439 | 1728161422 | 1822471558 | 1788060072 | 27931 | SRX9416877 | SRS7632325 | SRA1152184 | Universidad de las Americas|Facultad de Medicina Veterinaria y Agronomia | Universidad de las Americas | 2 | 0.95713 | 0.95761 | 0.04562 | 0.04518 | 0.77339 | 0.7725 | 0.54579 | 0.55214 | 151 | 151 | B | B | biological fallback assumption | illumina | hiseq_era | unknown | random_priming | trueseq | bulk | unknown | unknown | Unknown | 2020-11-02 | Adult | Adult | Gut | Digestive System | |||||||||||||||||||||
| 61695 | 61695 | SRR12964275 | SRX9416876 | SRS7632325 | SRP290781 | PRJNA673693 | Global studies of soybean meal dietary in fish | PRJNA673693 | Other | Intestinal transcriptome profile to evaluate dietary soybean meal tolerance favoring growth in adult zebrafish | LG | strain:family generated from TAB 5 DB 3 or AB|isolate:null|breed:zebrafish|age:98 dpf stage:adult|sex:male|tissue:intestine|biomaterial provider:Danio Biotech Company from Zebrafish for Innovation and Research ZIRLab Faculty of Sciences at University of Chile|genotype:low w8 post a soy diet|BioSampleModel:Model organism or animal | RNA Seq of zebrafish adult soy diet | 13 6 R1 | 13 6 R1 | RNA Seq libraries were constructed using TruSeq Stranded mRNA LT Sample Prep Kit following TruSeq Stranded mRNA Sample Preparation Guide Part #15031047 Rev. E. Briefly each sequencing library was prepared by random fragmentation of the DNA or cDNA sample followed by five prime and three prime adapter ligation. Alternatively "tagmentation" combines the fragmentation and ligation reactions into a single step that greatly increases the efficiency of the library preparation process. Adapter ligated fragments were then PCR amplified and gel purified. The fragments were sequenced with a read length of 151 base pairs using Illumina sequencing technology | RNA-Seq | TRANSCRIPTOMIC | RANDOM | PAIRED | ILLUMINA | Illumina HiSeq 1000 | SRP290781 | F13-6-CH_1.fastq.gz F13-6-CH_2.fastq.gz | fastq fastq | 6717305366.0 | 22242733.0 | F13 6 CH 1.fastq.gz | 0:151 1:151 | A:1751406615;C:1600949148;G:1664039061;T:1700884996;N:25546 | 151 | 151 | 1751406615 | 1600949148 | 1664039061 | 1700884996 | 25546 | SRX9416876 | SRS7632325 | SRA1152184 | Universidad de las Americas|Facultad de Medicina Veterinaria y Agronomia | Universidad de las Americas | 2 | 0.95426 | 0.95523 | 0.05393 | 0.0534 | 0.76165 | 0.76045 | 0.53973 | 0.54233 | 151 | 151 | B | B | biological fallback assumption | illumina | hiseq_era | unknown | random_priming | trueseq | bulk | unknown | unknown | Unknown | 2020-11-02 | Adult | Adult | Gut | Digestive System | |||||||||||||||||||||
| 61696 | 61696 | SRR12964276 | SRX9416875 | SRS7632325 | SRP290781 | PRJNA673693 | Global studies of soybean meal dietary in fish | PRJNA673693 | Other | Intestinal transcriptome profile to evaluate dietary soybean meal tolerance favoring growth in adult zebrafish | LG | strain:family generated from TAB 5 DB 3 or AB|isolate:null|breed:zebrafish|age:98 dpf stage:adult|sex:male|tissue:intestine|biomaterial provider:Danio Biotech Company from Zebrafish for Innovation and Research ZIRLab Faculty of Sciences at University of Chile|genotype:low w8 post a soy diet|BioSampleModel:Model organism or animal | RNA Seq of zebrafish adult soy diet | 20 4 R1 | 20 4 R1 | RNA Seq libraries were constructed using TruSeq Stranded mRNA LT Sample Prep Kit following TruSeq Stranded mRNA Sample Preparation Guide Part #15031047 Rev. E. Briefly each sequencing library was prepared by random fragmentation of the DNA or cDNA sample followed by five prime and three prime adapter ligation. Alternatively "tagmentation" combines the fragmentation and ligation reactions into a single step that greatly increases the efficiency of the library preparation process. Adapter ligated fragments were then PCR amplified and gel purified. The fragments were sequenced with a read length of 151 base pairs using Illumina sequencing technology | RNA-Seq | TRANSCRIPTOMIC | RANDOM | PAIRED | ILLUMINA | Illumina HiSeq 1000 | SRP290781 | F20-4-CH_1.fastq.gz F20-4-CH_2.fastq.gz | fastq fastq | 8278093914.0 | 27410907.0 | F20 4 CH 1.fastq.gz | 0:151 1:151 | A:2152723831;C:1979642795;G:2075202406;T:2070493116;N:31766 | 151 | 151 | 2152723831 | 1979642795 | 2075202406 | 2070493116 | 31766 | SRX9416875 | SRS7632325 | SRA1152184 | Universidad de las Americas|Facultad de Medicina Veterinaria y Agronomia | Universidad de las Americas | 2 | 0.95671 | 0.9578 | 0.04994 | 0.04962 | 0.76282 | 0.76311 | 0.54822 | 0.55628 | 151 | 151 | B | B | biological fallback assumption | illumina | hiseq_era | unknown | random_priming | trueseq | bulk | unknown | unknown | Unknown | 2020-11-02 | Adult | Adult | Gut | Digestive System | |||||||||||||||||||||
| 61697 | 61697 | SRR12964277 | SRX9416874 | SRS7632325 | SRP290781 | PRJNA673693 | Global studies of soybean meal dietary in fish | PRJNA673693 | Other | Intestinal transcriptome profile to evaluate dietary soybean meal tolerance favoring growth in adult zebrafish | LG | strain:family generated from TAB 5 DB 3 or AB|isolate:null|breed:zebrafish|age:98 dpf stage:adult|sex:male|tissue:intestine|biomaterial provider:Danio Biotech Company from Zebrafish for Innovation and Research ZIRLab Faculty of Sciences at University of Chile|genotype:low w8 post a soy diet|BioSampleModel:Model organism or animal | RNA Seq of zebrafish adult soy diet | 22 3 R1 | 22 3 R1 | RNA Seq libraries were constructed using TruSeq Stranded mRNA LT Sample Prep Kit following TruSeq Stranded mRNA Sample Preparation Guide Part #15031047 Rev. E. Briefly each sequencing library was prepared by random fragmentation of the DNA or cDNA sample followed by five prime and three prime adapter ligation. Alternatively "tagmentation" combines the fragmentation and ligation reactions into a single step that greatly increases the efficiency of the library preparation process. Adapter ligated fragments were then PCR amplified and gel purified. The fragments were sequenced with a read length of 151 base pairs using Illumina sequencing technology | RNA-Seq | TRANSCRIPTOMIC | RANDOM | PAIRED | ILLUMINA | Illumina HiSeq 1000 | SRP290781 | F22-3-CH_1.fastq.gz F22-3-CH_2.fastq.gz | fastq fastq | 6744916924.0 | 22334162.0 | F22 3 CH 1.fastq.gz | 0:151 1:151 | A:1735435964;C:1625676259;G:1701661616;T:1682117454;N:25631 | 151 | 151 | 1735435964 | 1625676259 | 1701661616 | 1682117454 | 25631 | SRX9416874 | SRS7632325 | SRA1152184 | Universidad de las Americas|Facultad de Medicina Veterinaria y Agronomia | Universidad de las Americas | 2 | 0.96162 | 0.96291 | 0.04811 | 0.04761 | 0.76731 | 0.76597 | 0.54763 | 0.55415 | 151 | 151 | B | B | biological fallback assumption | illumina | hiseq_era | unknown | random_priming | trueseq | bulk | unknown | unknown | Unknown | 2020-11-02 | Adult | Adult | Gut | Digestive System | |||||||||||||||||||||
| 61698 | 61698 | SRR12964278 | SRX9416873 | SRS7632325 | SRP290781 | PRJNA673693 | Global studies of soybean meal dietary in fish | PRJNA673693 | Other | Intestinal transcriptome profile to evaluate dietary soybean meal tolerance favoring growth in adult zebrafish | LG | strain:family generated from TAB 5 DB 3 or AB|isolate:null|breed:zebrafish|age:98 dpf stage:adult|sex:male|tissue:intestine|biomaterial provider:Danio Biotech Company from Zebrafish for Innovation and Research ZIRLab Faculty of Sciences at University of Chile|genotype:low w8 post a soy diet|BioSampleModel:Model organism or animal | RNA Seq of zebrafish adult soy diet | 22 5 R1 | 22 5 R1 | RNA Seq libraries were constructed using TruSeq Stranded mRNA LT Sample Prep Kit following TruSeq Stranded mRNA Sample Preparation Guide Part #15031047 Rev. E. Briefly each sequencing library was prepared by random fragmentation of the DNA or cDNA sample followed by five prime and three prime adapter ligation. Alternatively "tagmentation" combines the fragmentation and ligation reactions into a single step that greatly increases the efficiency of the library preparation process. Adapter ligated fragments were then PCR amplified and gel purified. The fragments were sequenced with a read length of 151 base pairs using Illumina sequencing technology | RNA-Seq | TRANSCRIPTOMIC | RANDOM | PAIRED | ILLUMINA | Illumina HiSeq 1000 | SRP290781 | F22-5-CH_1.fastq.gz F22-5-CH_2.fastq.gz | fastq fastq | 9233983710.0 | 30576105.0 | F22 5 CH 1.fastq.gz | 0:151 1:151 | A:2414399726;C:2199826316;G:2268243787;T:2351478779;N:35102 | 151 | 151 | 2414399726 | 2199826316 | 2268243787 | 2351478779 | 35102 | SRX9416873 | SRS7632325 | SRA1152184 | Universidad de las Americas|Facultad de Medicina Veterinaria y Agronomia | Universidad de las Americas | 2 | 0.95807 | 0.95965 | 0.05505 | 0.05432 | 0.74878 | 0.74775 | 0.54766 | 0.53823 | 151 | 151 | B | B | biological fallback assumption | illumina | hiseq_era | unknown | random_priming | trueseq | bulk | unknown | unknown | Unknown | 2020-11-02 | Adult | Adult | Gut | Digestive System | |||||||||||||||||||||
| 61699 | 61699 | SRR12964279 | SRX9416872 | SRS7632324 | SRP290781 | PRJNA673693 | Global studies of soybean meal dietary in fish | PRJNA673693 | Other | Intestinal transcriptome profile to evaluate dietary soybean meal tolerance favoring growth in adult zebrafish | HG | strain:family generated from TAB 5 DB 3 or AB|isolate:null|breed:zebrafish|age:98 dpf stage:adult|sex:male|tissue:intestine|biomaterial provider:Danio Biotech Company from Zebrafish for Innovation and Research ZIRLab Faculty of Sciences at University of Chile|genotype:high w8 post a soy diet|BioSampleModel:Model organism or animal | RNA Seq of zebrafish adult soy diet | 3 7 R1 | 3 7 R1 | RNA Seq libraries were constructed using TruSeq Stranded mRNA LT Sample Prep Kit following TruSeq Stranded mRNA Sample Preparation Guide Part #15031047 Rev. E. Briefly each sequencing library was prepared by random fragmentation of the DNA or cDNA sample followed by five prime and three prime adapter ligation. Alternatively "tagmentation" combines the fragmentation and ligation reactions into a single step that greatly increases the efficiency of the library preparation process. Adapter ligated fragments were then PCR amplified and gel purified. The fragments were sequenced with a read length of 151 base pairs using Illumina sequencing technology | RNA-Seq | TRANSCRIPTOMIC | RANDOM | PAIRED | ILLUMINA | Illumina HiSeq 1000 | SRP290781 | F3-7-GR_1.fastq.gz F3-7-GR_2.fastq.gz | fastq fastq | 6885663118.0 | 22800209.0 | F3 7 GR 1.fastq.gz | 0:151 1:151 | A:1812477779;C:1623127887;G:1690116325;T:1759914694;N:26433 | 151 | 151 | 1812477779 | 1623127887 | 1690116325 | 1759914694 | 26433 | SRX9416872 | SRS7632324 | SRA1152184 | Universidad de las Americas|Facultad de Medicina Veterinaria y Agronomia | Universidad de las Americas | 2 | 0.94927 | 0.95113 | 0.05683 | 0.05648 | 0.75191 | 0.7512 | 0.54881 | 0.52947 | 151 | 151 | B | B | biological fallback assumption | illumina | hiseq_era | unknown | random_priming | trueseq | bulk | unknown | unknown | Unknown | 2020-11-02 | Adult | Adult | Gut | Digestive System | |||||||||||||||||||||
| 61700 | 61700 | SRR12964280 | SRX9416871 | SRS7632324 | SRP290781 | PRJNA673693 | Global studies of soybean meal dietary in fish | PRJNA673693 | Other | Intestinal transcriptome profile to evaluate dietary soybean meal tolerance favoring growth in adult zebrafish | HG | strain:family generated from TAB 5 DB 3 or AB|isolate:null|breed:zebrafish|age:98 dpf stage:adult|sex:male|tissue:intestine|biomaterial provider:Danio Biotech Company from Zebrafish for Innovation and Research ZIRLab Faculty of Sciences at University of Chile|genotype:high w8 post a soy diet|BioSampleModel:Model organism or animal | RNA Seq of zebrafish adult soy diet | 12 8 R1 | 12 8 R1 | RNA Seq libraries were constructed using TruSeq Stranded mRNA LT Sample Prep Kit following TruSeq Stranded mRNA Sample Preparation Guide Part #15031047 Rev. E. Briefly each sequencing library was prepared by random fragmentation of the DNA or cDNA sample followed by five prime and three prime adapter ligation. Alternatively "tagmentation" combines the fragmentation and ligation reactions into a single step that greatly increases the efficiency of the library preparation process. Adapter ligated fragments were then PCR amplified and gel purified. The fragments were sequenced with a read length of 151 base pairs using Illumina sequencing technology | RNA-Seq | TRANSCRIPTOMIC | RANDOM | PAIRED | ILLUMINA | Illumina HiSeq 1000 | SRP290781 | F12-8-GR_1.fastq.gz F12-8-GR_2.fastq.gz | fastq fastq | 7444642166.0 | 24651133.0 | F12 8 GR 1.fastq.gz | 0:151 1:151 | A:1929248444;C:1789983189;G:1819316431;T:1906065632;N:28470 | 151 | 151 | 1929248444 | 1789983189 | 1819316431 | 1906065632 | 28470 | SRX9416871 | SRS7632324 | SRA1152184 | Universidad de las Americas|Facultad de Medicina Veterinaria y Agronomia | Universidad de las Americas | 2 | 0.96276 | 0.96524 | 0.05604 | 0.05528 | 0.75684 | 0.75564 | 0.52829 | 0.54166 | 151 | 151 | B | B | biological fallback assumption | illumina | hiseq_era | unknown | random_priming | trueseq | bulk | unknown | unknown | Unknown | 2020-11-02 | Adult | Adult | Gut | Digestive System | |||||||||||||||||||||
| 61701 | 61701 | SRR12964281 | SRX9416870 | SRS7632324 | SRP290781 | PRJNA673693 | Global studies of soybean meal dietary in fish | PRJNA673693 | Other | Intestinal transcriptome profile to evaluate dietary soybean meal tolerance favoring growth in adult zebrafish | HG | strain:family generated from TAB 5 DB 3 or AB|isolate:null|breed:zebrafish|age:98 dpf stage:adult|sex:male|tissue:intestine|biomaterial provider:Danio Biotech Company from Zebrafish for Innovation and Research ZIRLab Faculty of Sciences at University of Chile|genotype:high w8 post a soy diet|BioSampleModel:Model organism or animal | RNA Seq of zebrafish adult soy diet | 3 9 R1 | 3 9 R1 | RNA Seq libraries were constructed using TruSeq Stranded mRNA LT Sample Prep Kit following TruSeq Stranded mRNA Sample Preparation Guide Part #15031047 Rev. E. Briefly each sequencing library was prepared by random fragmentation of the DNA or cDNA sample followed by five prime and three prime adapter ligation. Alternatively "tagmentation" combines the fragmentation and ligation reactions into a single step that greatly increases the efficiency of the library preparation process. Adapter ligated fragments were then PCR amplified and gel purified. The fragments were sequenced with a read length of 151 base pairs using Illumina sequencing technology | RNA-Seq | TRANSCRIPTOMIC | RANDOM | PAIRED | ILLUMINA | Illumina HiSeq 1000 | SRP290781 | F3-9-GR_1.fastq.gz F3-9-GR_2.fastq.gz | fastq fastq | 8809498550.0 | 29170525.0 | F3 9 GR 1.fastq.gz | 0:151 1:151 | A:2306194345;C:2098546233;G:2173624200;T:2231100146;N:33626 | 151 | 151 | 2306194345 | 2098546233 | 2173624200 | 2231100146 | 33626 | SRX9416870 | SRS7632324 | SRA1152184 | Universidad de las Americas|Facultad de Medicina Veterinaria y Agronomia | Universidad de las Americas | 2 | 0.95932 | 0.96111 | 0.05271 | 0.05227 | 0.7514 | 0.75097 | 0.55339 | 0.55085 | 151 | 151 | B | B | biological fallback assumption | illumina | hiseq_era | unknown | random_priming | trueseq | bulk | unknown | unknown | Unknown | 2020-11-02 | Adult | Adult | Gut | Digestive System | |||||||||||||||||||||
| 61702 | 61702 | SRR12964282 | SRX9416869 | SRS7632324 | SRP290781 | PRJNA673693 | Global studies of soybean meal dietary in fish | PRJNA673693 | Other | Intestinal transcriptome profile to evaluate dietary soybean meal tolerance favoring growth in adult zebrafish | HG | strain:family generated from TAB 5 DB 3 or AB|isolate:null|breed:zebrafish|age:98 dpf stage:adult|sex:male|tissue:intestine|biomaterial provider:Danio Biotech Company from Zebrafish for Innovation and Research ZIRLab Faculty of Sciences at University of Chile|genotype:high w8 post a soy diet|BioSampleModel:Model organism or animal | RNA Seq of zebrafish adult soy diet | 21 7 R1 | 21 7 R1 | RNA Seq libraries were constructed using TruSeq Stranded mRNA LT Sample Prep Kit following TruSeq Stranded mRNA Sample Preparation Guide Part #15031047 Rev. E. Briefly each sequencing library was prepared by random fragmentation of the DNA or cDNA sample followed by five prime and three prime adapter ligation. Alternatively "tagmentation" combines the fragmentation and ligation reactions into a single step that greatly increases the efficiency of the library preparation process. Adapter ligated fragments were then PCR amplified and gel purified. The fragments were sequenced with a read length of 151 base pairs using Illumina sequencing technology | RNA-Seq | TRANSCRIPTOMIC | RANDOM | PAIRED | ILLUMINA | Illumina HiSeq 1000 | SRP290781 | F21-7-GR_1.fastq.gz F21-7-GR_2.fastq.gz | fastq fastq | 9035029734.0 | 29917317.0 | F21 7 GR 1.fastq.gz | 0:151 1:151 | A:2358940333;C:2147949025;G:2221572455;T:2306533838;N:34083 | 151 | 151 | 2358940333 | 2147949025 | 2221572455 | 2306533838 | 34083 | SRX9416869 | SRS7632324 | SRA1152184 | Universidad de las Americas|Facultad de Medicina Veterinaria y Agronomia | Universidad de las Americas | 2 | 0.94873 | 0.95017 | 0.05513 | 0.05454 | 0.75073 | 0.75061 | 0.54564 | 0.55234 | 151 | 151 | B | B | biological fallback assumption | illumina | hiseq_era | unknown | random_priming | trueseq | bulk | unknown | unknown | Unknown | 2020-11-02 | Adult | Adult | Gut | Digestive System | |||||||||||||||||||||
| 61703 | 61703 | SRR12964283 | SRX9416868 | SRS7632324 | SRP290781 | PRJNA673693 | Global studies of soybean meal dietary in fish | PRJNA673693 | Other | Intestinal transcriptome profile to evaluate dietary soybean meal tolerance favoring growth in adult zebrafish | HG | strain:family generated from TAB 5 DB 3 or AB|isolate:null|breed:zebrafish|age:98 dpf stage:adult|sex:male|tissue:intestine|biomaterial provider:Danio Biotech Company from Zebrafish for Innovation and Research ZIRLab Faculty of Sciences at University of Chile|genotype:high w8 post a soy diet|BioSampleModel:Model organism or animal | RNA Seq of zebrafish adult soy diet | 21 1 R1 | 21 1 R1 | RNA Seq libraries were constructed using TruSeq Stranded mRNA LT Sample Prep Kit following TruSeq Stranded mRNA Sample Preparation Guide Part #15031047 Rev. E. Briefly each sequencing library was prepared by random fragmentation of the DNA or cDNA sample followed by five prime and three prime adapter ligation. Alternatively "tagmentation" combines the fragmentation and ligation reactions into a single step that greatly increases the efficiency of the library preparation process. Adapter ligated fragments were then PCR amplified and gel purified. The fragments were sequenced with a read length of 151 base pairs using Illumina sequencing technology | RNA-Seq | TRANSCRIPTOMIC | RANDOM | PAIRED | ILLUMINA | Illumina HiSeq 1000 | SRP290781 | F21-1-GR_1.fastq.gz F21-1-GR_2.fastq.gz | fastq fastq | 8985193090.0 | 29752295.0 | F21 1 GR 1.fastq.gz | 0:151 1:151 | A:2309244915;C:2172345682;G:2225381374;T:2278186696;N:34423 | 151 | 151 | 2309244915 | 2172345682 | 2225381374 | 2278186696 | 34423 | SRX9416868 | SRS7632324 | SRA1152184 | Universidad de las Americas|Facultad de Medicina Veterinaria y Agronomia | Universidad de las Americas | 2 | 0.95801 | 0.96086 | 0.05089 | 0.05045 | 0.75643 | 0.7554 | 0.54732 | 0.51671 | 151 | 151 | B | B | biological fallback assumption | illumina | hiseq_era | unknown | random_priming | trueseq | bulk | unknown | unknown | Unknown | 2020-11-02 | Adult | Adult | Gut | Digestive System | |||||||||||||||||||||
| 71597 | 71597 | SRR21780858 | SRX17775679 | SRS15301946 | SRP400655 | PRJNA885906 | Cell Type Specific Responses to the Microbiota Across All Tissues of the Larval Zebrafish | PRJNA885906 | Other | The transcriptional responses of the host zebrafish to the presence of the microbiota was assessed across all cell types throughout the larval body at single cell resolution. The goal of this work is to generate a resource for generating new hypotheses about the intricate interactions between animal hosts and their microbiota. | BefA diss | BefA 1532 | strain:Tgins; eGFP|dev stage:6dpf|sex:not applicable|tissue:dissected digestive systems|BioSampleModel:Model organism or animal | germ free 6dpf dissected digestive systems treated with protein | 1532 | 1532 | the cells are from dissected guts from germ free larvae but were treated with exogenous protein | RNA-Seq | TRANSCRIPTOMIC SINGLE CELL | RANDOM | PAIRED | ILLUMINA | Illumina HiSeq 4000 | SRP400655 | BefAdiss_S2_L008_R1_001.fastq.gz BefAdiss_S2_L008_R2_001.fastq.gz | fastq fastq | 62618506720.0 | 355786970.0 | BefAdiss S2 L008 R1 001.fastq.gz | 0:26 1:150 | A:18434729847;C:13374700052;G:13922149278;T:16789619859;N:97307684 | 26 | 150 | 18434729847 | 13374700052 | 13922149278 | 16789619859 | 97307684 | SRX17775679 | SRS15301946 | SRA1511285 | University of Oregon|Institute of Molecular Biology | University of Oregon | 2 | 0.00182 | 0.89692 | 0.00064 | 0.07716 | 0.9962 | 0.82798 | 0.42608 | 0.6075 | 26 | 150 | T | B | sc-like readlen | illumina | hiseq_era | unknown | random_priming | unknown | sc | unknown | unknown | United States | 2022-10-02 | Larval | Larval | Gut | Digestive System | ||||||||||||||||||||
| 71598 | 71598 | SRR21780859 | SRX17775678 | SRS15301945 | SRP400655 | PRJNA885906 | Cell Type Specific Responses to the Microbiota Across All Tissues of the Larval Zebrafish | PRJNA885906 | Other | The transcriptional responses of the host zebrafish to the presence of the microbiota was assessed across all cell types throughout the larval body at single cell resolution. The goal of this work is to generate a resource for generating new hypotheses about the intricate interactions between animal hosts and their microbiota. | GF diss2 | GF 1531 | strain:Tgins; eGFP|dev stage:6dpf|sex:not applicable|tissue:dissected digestive systems|BioSampleModel:Model organism or animal | germ free 6dpf dissected digestive systems lane 2 | 1531 | 1531 | the cells dissected guts from germ free larvae were split across two lanes library 1530/1531 come from same sample | RNA-Seq | TRANSCRIPTOMIC SINGLE CELL | RANDOM | PAIRED | ILLUMINA | Illumina HiSeq 4000 | SRP400655 | GFdiss2_S1_L004_R1_001.fastq.gz GFdiss2_S1_L004_R2_001.fastq.gz | fastq fastq | 66862372720.0 | 379899845.0 | GFdiss2 S1 L004 R1 001.fastq.gz | 0:26 1:150 | A:10814720171;C:8609009803;G:8785194946;T:9942289999;N:28711157801 | 26 | 150 | 10814720171 | 8609009803 | 8785194946 | 9942289999 | 28711157801 | SRX17775678 | SRS15301945 | SRA1511285 | University of Oregon|Institute of Molecular Biology | University of Oregon | 2 | 0.00191 | 0.92014 | 0.00074 | 0.08575 | 0.99616 | 0.87793 | 0.35964 | 0.58459 | 26 | 150 | T | B | sc-like readlen | illumina | hiseq_era | unknown | random_priming | unknown | sc | unknown | unknown | United States | 2022-10-02 | Larval | Larval | Gut | Digestive System | ||||||||||||||||||||
| 71599 | 71599 | SRR21780860 | SRX17775677 | SRS15301944 | SRP400655 | PRJNA885906 | Cell Type Specific Responses to the Microbiota Across All Tissues of the Larval Zebrafish | PRJNA885906 | Other | The transcriptional responses of the host zebrafish to the presence of the microbiota was assessed across all cell types throughout the larval body at single cell resolution. The goal of this work is to generate a resource for generating new hypotheses about the intricate interactions between animal hosts and their microbiota. | GF diss1 | GF 1530 | strain:Tgins; eGFP|dev stage:6dpf|sex:not applicable|tissue:dissected digestive systems|BioSampleModel:Model organism or animal | germ free 6dpf dissected digestive systems lane 1 | 1530 | 1530 | the cells dissected guts from germ free larvae were split across two lanes library 1530/1531 come from same sample | RNA-Seq | TRANSCRIPTOMIC SINGLE CELL | RANDOM | PAIRED | ILLUMINA | Illumina HiSeq 4000 | SRP400655 | GFdiss1_S1_L007_R1_001.fastq.gz GFdiss1_S1_L007_R2_001.fastq.gz | fastq fastq | 63769716736.0 | 362327936.0 | GFdiss1 S1 L007 R1 001.fastq.gz | 0:26 1:150 | A:18291156920;C:14095548431;G:14421791117;T:16786925671;N:174294597 | 26 | 150 | 18291156920 | 14095548431 | 14421791117 | 16786925671 | 174294597 | SRX17775677 | SRS15301944 | SRA1511285 | University of Oregon|Institute of Molecular Biology | University of Oregon | 2 | 0.00172 | 0.91725 | 0.00064 | 0.08763 | 0.99648 | 0.82804 | 0.39234 | 0.5982 | 26 | 150 | T | B | sc-like readlen | illumina | hiseq_era | unknown | random_priming | unknown | sc | unknown | unknown | United States | 2022-10-02 | Larval | Larval | Gut | Digestive System |
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CREATE TABLE run_metadata("run.accession" VARCHAR, "experiment.accession" VARCHAR, "sample.accession" VARCHAR, "study.accession" VARCHAR, bioproject VARCHAR, "study.title" VARCHAR, "study.alias" VARCHAR, "study.type" VARCHAR, "study.abstract" VARCHAR, "study.attributes" VARCHAR, "study.PMIDs" VARCHAR, "sample.description" VARCHAR, "sample.title" VARCHAR, "sample.alias" VARCHAR, "sample.centername" VARCHAR, "sample.attributes" VARCHAR, "GEOsample.title" VARCHAR, "GEOsample.dataprocessing" VARCHAR, "GEOsample.source" VARCHAR, "GEOsample.treatmentprotocol" VARCHAR, "GEOsample.extractprotocol" VARCHAR, "GEOsample.growthprotocol" VARCHAR, "GEOsample.characteristics" VARCHAR, "GEOsample.accession" VARCHAR, "experiment.title" VARCHAR, "experiment.alias" VARCHAR, "experiment.library_name" VARCHAR, "experiment.design_description" VARCHAR, "experiment.library_construction_protocol" VARCHAR, "experiment.attributes" VARCHAR, "experiment.library_strategy" VARCHAR, "experiment.library_source" VARCHAR, "experiment.library_selection" VARCHAR, "experiment.library_layout" VARCHAR, "experiment.platform" VARCHAR, "experiment.instrument_model" VARCHAR, "experiment.spot_descriptor" VARCHAR, "experiment.study_ref" VARCHAR, "run.title" VARCHAR, "run.attributes" VARCHAR, "run.filename" VARCHAR, "run.semantic_name" VARCHAR, "run.total_bases" DOUBLE, "run.total_spots" DOUBLE, "run.alias" VARCHAR, "run.read_lengths" VARCHAR, "run.base_counts" VARCHAR, "run.r1_length" BIGINT, "run.r2_length" BIGINT, "run.r3_length" BIGINT, "run.r4_length" BIGINT, "run.Acount" BIGINT, "run.Ccount" BIGINT, "run.Gcount" BIGINT, "run.Tcount" BIGINT, "run.Ncount" BIGINT, "run.experiment" VARCHAR, "run.pool_member" VARCHAR, "submission.accession" VARCHAR, "submission.srasource" VARCHAR, "submission.bioprojectsource" VARCHAR, "seqdetective.n_mates" BIGINT, "seqdetective.mapping_rate.mate1" DOUBLE, "seqdetective.mapping_rate.mate2" DOUBLE, "seqdetective.nofeature_rate.mate1" DOUBLE, "seqdetective.nofeature_rate.mate2" DOUBLE, "seqdetective.sparsity.mate1" DOUBLE, "seqdetective.sparsity.mate2" DOUBLE, "seqdetective.pos_strand_rate.mate1" DOUBLE, "seqdetective.pos_strand_rate.mate2" DOUBLE, "seqdetective.readlen.mate1" BIGINT, "seqdetective.readlen.mate2" BIGINT, "seqdetective.judgement.mate1" VARCHAR, "seqdetective.judgement.mate2" VARCHAR, "seqdetective.judgement.reason" VARCHAR, platform_family VARCHAR, instrument_generation VARCHAR, read_bias VARCHAR, selection_class VARCHAR, prep_kit VARCHAR, sc_or_bulk VARCHAR, tech_class VARCHAR, technology VARCHAR, tech_variant VARCHAR, "submission.bioprojectsource.country" VARCHAR, earliest_date DATE, devstage_curation VARCHAR, devstage_curation_coarse VARCHAR, tissue_curation VARCHAR, tissue_curation_coarse VARCHAR);;
CREATE INDEX idx_run_bioproject ON run_metadata(bioproject);;
CREATE INDEX idx_run_run_accession ON run_metadata("run.accession");;