run_metadata
45 rows where experiment.library_selection = "RACE", experiment.library_source = "TRANSCRIPTOMIC" and tissue_curation = "Whole Organism"
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| Link | rowid ▼ | run.accession | experiment.accession | sample.accession | study.accession | bioproject | study.title | study.alias | study.type | study.abstract | study.attributes | study.PMIDs | sample.description | sample.title | sample.alias | sample.centername | sample.attributes | GEOsample.title | GEOsample.dataprocessing | GEOsample.source | GEOsample.treatmentprotocol | GEOsample.extractprotocol | GEOsample.growthprotocol | GEOsample.characteristics | GEOsample.accession | experiment.title | experiment.alias | experiment.library_name | experiment.design_description | experiment.library_construction_protocol | experiment.attributes | experiment.library_strategy | experiment.library_source | experiment.library_selection | experiment.library_layout | experiment.platform | experiment.instrument_model | experiment.spot_descriptor | experiment.study_ref | run.title | run.attributes | run.filename | run.semantic_name | run.total_bases | run.total_spots | run.alias | run.read_lengths | run.base_counts | run.r1_length | run.r2_length | run.r3_length | run.r4_length | run.Acount | run.Ccount | run.Gcount | run.Tcount | run.Ncount | run.experiment | run.pool_member | submission.accession | submission.srasource | submission.bioprojectsource | seqdetective.n_mates | seqdetective.mapping_rate.mate1 | seqdetective.mapping_rate.mate2 | seqdetective.nofeature_rate.mate1 | seqdetective.nofeature_rate.mate2 | seqdetective.sparsity.mate1 | seqdetective.sparsity.mate2 | seqdetective.pos_strand_rate.mate1 | seqdetective.pos_strand_rate.mate2 | seqdetective.readlen.mate1 | seqdetective.readlen.mate2 | seqdetective.judgement.mate1 | seqdetective.judgement.mate2 | seqdetective.judgement.reason | platform_family | instrument_generation | read_bias | selection_class | prep_kit | sc_or_bulk | tech_class | technology | tech_variant | submission.bioprojectsource.country | earliest_date | devstage_curation | devstage_curation_coarse | tissue_curation | tissue_curation_coarse |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 10141 | 10141 | ERR4973564 | ERX4792138 | ERS5459722 | ERP125703 | PRJEB41864 | three prime mRNA seq of sfpq / zebrafish embryos and siblings at 24 hpf | E-MTAB-9899 | Transcriptome Analysis | mRNA was purified from whole zebrafish embryos at 24 hpf and three prime proximal region was sequenced. | ENA FIRST PUBLIC:2021 01 27|ENA LAST UPDATE:2020 12 10 | Protocols: Embryos were collected from in crosses of sfpq+/ adult zebrafish RNA was extracted using the RNEasy Mini Kit Qiagen Library was generated using Lexogen's QuantSeq three prime mRNA Seq Library Prep Kit FWD for Illumina | sibling 3 | SAMEA7703213 | Centre for Developmental Neurobiology King's College London | ENA first public:2021 01 27|ENA last update:2020 12 10|External Id:SAMEA7703213|INSDC center alias:Centre for Developmental Neurobiology King's College London|INSDC center name:Centre for Developmental Neurobiology King's College London|INSDC first public:2021 01 27T17:06:57Z|INSDC last update:2020 12 10T17:23:32Z|INSDC status:public|Submitter Id:E MTAB 9899:sibling 3|age:24|broker name:ArrayExpress|common name:zebrafish|developmental stage:embryo stage|genotype:wild type genotype|organism part:whole organism|sample name:E MTAB 9899:sibling 3 | NextSeq 500 sequencing; 3 prime mRNA seq of sfpq / zebrafish embryos and siblings at 24 hpf | E MTAB 9899:sibling 3 s | sibling 3 s | three prime mRNA seq of sfpq / zebrafish embryos and siblings at 24 hpf | Embryos were collected from in crosses of sfpq+/ adult zebrafish RNA was extracted using the RNEasy Mini Kit Qiagen Library was generated using Lexogen's QuantSeq three prime mRNA Seq Library Prep Kit FWD for Illumina | Experimental Factor: genotype:wild type genotype | RNA-Seq | TRANSCRIPTOMIC | RACE | SINGLE | ILLUMINA | NextSeq 500 | ERP125703 | NextSeq 500 sequencing; three prime mRNA seq of sfpq / zebrafish embryos and siblings at 24 hpf | ENA FIRST PUBLIC:2021 01 27|ENA LAST UPDATE:2020 12 10 | 70_AACCGA_70_ACTAGC_S2_R1_001.fastq.gz | fastq | E MTAB 9899:sibling 3 | 0:76 1:0 | A:385382600;C:210104151;G:238678838;T:315495923;N:5536 | 76 | 0 | 385382600 | 210104151 | 238678838 | 315495923 | 5536 | ERX4792138 | ERS5459722 | ERA3194022 | Centre for Developmental Neurobiology King | Centre for Developmental Neurobiology King | 1 | 0.82613 | 0.13111 | 0.77597 | 0.53833 | 76 | B | usable mapping rate | illumina | nextseq | 3prime | other | lexogen | bulk | unknown | unknown | United Kingdom | 2020-12-10 | Multi-stage | Multi-stage | Whole Organism | All anatomical structures | |||||||||||||||||||||
| 10142 | 10142 | ERR4973563 | ERX4792137 | ERS5459721 | ERP125703 | PRJEB41864 | three prime mRNA seq of sfpq / zebrafish embryos and siblings at 24 hpf | E-MTAB-9899 | Transcriptome Analysis | mRNA was purified from whole zebrafish embryos at 24 hpf and three prime proximal region was sequenced. | ENA FIRST PUBLIC:2021 01 27|ENA LAST UPDATE:2020 12 10 | Protocols: Embryos were collected from in crosses of sfpq+/ adult zebrafish RNA was extracted using the RNEasy Mini Kit Qiagen Library was generated using Lexogen's QuantSeq three prime mRNA Seq Library Prep Kit FWD for Illumina | sibling 2 | SAMEA7703212 | Centre for Developmental Neurobiology King's College London | ENA first public:2021 01 27|ENA last update:2020 12 10|External Id:SAMEA7703212|INSDC center alias:Centre for Developmental Neurobiology King's College London|INSDC center name:Centre for Developmental Neurobiology King's College London|INSDC first public:2021 01 27T17:06:57Z|INSDC last update:2020 12 10T17:23:32Z|INSDC status:public|Submitter Id:E MTAB 9899:sibling 2|age:24|broker name:ArrayExpress|common name:zebrafish|developmental stage:embryo stage|genotype:wild type genotype|organism part:whole organism|sample name:E MTAB 9899:sibling 2 | NextSeq 500 sequencing; 3 prime mRNA seq of sfpq / zebrafish embryos and siblings at 24 hpf | E MTAB 9899:sibling 2 s | sibling 2 s | three prime mRNA seq of sfpq / zebrafish embryos and siblings at 24 hpf | Embryos were collected from in crosses of sfpq+/ adult zebrafish RNA was extracted using the RNEasy Mini Kit Qiagen Library was generated using Lexogen's QuantSeq three prime mRNA Seq Library Prep Kit FWD for Illumina | Experimental Factor: genotype:wild type genotype | RNA-Seq | TRANSCRIPTOMIC | RACE | SINGLE | ILLUMINA | NextSeq 500 | ERP125703 | NextSeq 500 sequencing; three prime mRNA seq of sfpq / zebrafish embryos and siblings at 24 hpf | ENA FIRST PUBLIC:2021 01 27|ENA LAST UPDATE:2020 12 10 | 69_AGTTGA_69_CTTACA_S7_R1_001.fastq.gz | fastq | E MTAB 9899:sibling 2 | 0:76 1:0 | A:405337161;C:226099266;G:255734601;T:341120121;N:5955 | 76 | 0 | 405337161 | 226099266 | 255734601 | 341120121 | 5955 | ERX4792137 | ERS5459721 | ERA3194022 | Centre for Developmental Neurobiology King | Centre for Developmental Neurobiology King | 1 | 0.84066 | 0.12241 | 0.77755 | 0.56472 | 76 | B | usable mapping rate | illumina | nextseq | 3prime | other | lexogen | bulk | unknown | unknown | United Kingdom | 2020-12-10 | Multi-stage | Multi-stage | Whole Organism | All anatomical structures | |||||||||||||||||||||
| 10143 | 10143 | ERR4973562 | ERX4792136 | ERS5459720 | ERP125703 | PRJEB41864 | three prime mRNA seq of sfpq / zebrafish embryos and siblings at 24 hpf | E-MTAB-9899 | Transcriptome Analysis | mRNA was purified from whole zebrafish embryos at 24 hpf and three prime proximal region was sequenced. | ENA FIRST PUBLIC:2021 01 27|ENA LAST UPDATE:2020 12 10 | Protocols: Embryos were collected from in crosses of sfpq+/ adult zebrafish RNA was extracted using the RNEasy Mini Kit Qiagen Library was generated using Lexogen's QuantSeq three prime mRNA Seq Library Prep Kit FWD for Illumina | sibling 1 | SAMEA7703211 | Centre for Developmental Neurobiology King's College London | ENA first public:2021 01 27|ENA last update:2020 12 10|External Id:SAMEA7703211|INSDC center alias:Centre for Developmental Neurobiology King's College London|INSDC center name:Centre for Developmental Neurobiology King's College London|INSDC first public:2021 01 27T17:06:57Z|INSDC last update:2020 12 10T17:23:32Z|INSDC status:public|Submitter Id:E MTAB 9899:sibling 1|age:24|broker name:ArrayExpress|common name:zebrafish|developmental stage:embryo stage|genotype:wild type genotype|organism part:whole organism|sample name:E MTAB 9899:sibling 1 | NextSeq 500 sequencing; 3 prime mRNA seq of sfpq / zebrafish embryos and siblings at 24 hpf | E MTAB 9899:sibling 1 s | sibling 1 s | three prime mRNA seq of sfpq / zebrafish embryos and siblings at 24 hpf | Embryos were collected from in crosses of sfpq+/ adult zebrafish RNA was extracted using the RNEasy Mini Kit Qiagen Library was generated using Lexogen's QuantSeq three prime mRNA Seq Library Prep Kit FWD for Illumina | Experimental Factor: genotype:wild type genotype | RNA-Seq | TRANSCRIPTOMIC | RACE | SINGLE | ILLUMINA | NextSeq 500 | ERP125703 | NextSeq 500 sequencing; three prime mRNA seq of sfpq / zebrafish embryos and siblings at 24 hpf | ENA FIRST PUBLIC:2021 01 27|ENA LAST UPDATE:2020 12 10 | 68_CCAATT_68_GTCCCG_S11_R1_001.fastq.gz | fastq | E MTAB 9899:sibling 1 | 0:76 1:0 | A:440539801;C:236835299;G:268057277;T:355636994;N:6169 | 76 | 0 | 440539801 | 236835299 | 268057277 | 355636994 | 6169 | ERX4792136 | ERS5459720 | ERA3194022 | Centre for Developmental Neurobiology King | Centre for Developmental Neurobiology King | 1 | 0.82002 | 0.12908 | 0.77766 | 0.54981 | 76 | B | usable mapping rate | illumina | nextseq | 3prime | other | lexogen | bulk | unknown | unknown | United Kingdom | 2020-12-10 | Multi-stage | Multi-stage | Whole Organism | All anatomical structures | |||||||||||||||||||||
| 10144 | 10144 | ERR4973561 | ERX4792135 | ERS5459719 | ERP125703 | PRJEB41864 | three prime mRNA seq of sfpq / zebrafish embryos and siblings at 24 hpf | E-MTAB-9899 | Transcriptome Analysis | mRNA was purified from whole zebrafish embryos at 24 hpf and three prime proximal region was sequenced. | ENA FIRST PUBLIC:2021 01 27|ENA LAST UPDATE:2020 12 10 | Protocols: Embryos were collected from in crosses of sfpq+/ adult zebrafish RNA was extracted using the RNEasy Mini Kit Qiagen Library was generated using Lexogen's QuantSeq three prime mRNA Seq Library Prep Kit FWD for Illumina | sfpq 3 | SAMEA7703210 | Centre for Developmental Neurobiology King's College London | ENA first public:2021 01 27|ENA last update:2020 12 10|External Id:SAMEA7703210|INSDC center alias:Centre for Developmental Neurobiology King's College London|INSDC center name:Centre for Developmental Neurobiology King's College London|INSDC first public:2021 01 27T17:06:57Z|INSDC last update:2020 12 10T17:23:32Z|INSDC status:public|Submitter Id:E MTAB 9899:sfpq 3|age:24|broker name:ArrayExpress|common name:zebrafish|developmental stage:embryo stage|genotype:sfpq / |organism part:whole organism|sample name:E MTAB 9899:sfpq 3 | NextSeq 500 sequencing; 3 prime mRNA seq of sfpq / zebrafish embryos and siblings at 24 hpf | E MTAB 9899:sfpq 3 s | sfpq 3 s | three prime mRNA seq of sfpq / zebrafish embryos and siblings at 24 hpf | Embryos were collected from in crosses of sfpq+/ adult zebrafish RNA was extracted using the RNEasy Mini Kit Qiagen Library was generated using Lexogen's QuantSeq three prime mRNA Seq Library Prep Kit FWD for Illumina | Experimental Factor: genotype:sfpq / | RNA-Seq | TRANSCRIPTOMIC | RACE | SINGLE | ILLUMINA | NextSeq 500 | ERP125703 | NextSeq 500 sequencing; three prime mRNA seq of sfpq / zebrafish embryos and siblings at 24 hpf | ENA FIRST PUBLIC:2021 01 27|ENA LAST UPDATE:2020 12 10 | 67_TCGTTC_67_TGCTAT_S22_R1_001.fastq.gz | fastq | E MTAB 9899:sfpq 3 | 0:76 1:0 | A:493165327;C:268347233;G:303443918;T:399152596;N:7138 | 76 | 0 | 493165327 | 268347233 | 303443918 | 399152596 | 7138 | ERX4792135 | ERS5459719 | ERA3194022 | Centre for Developmental Neurobiology King | Centre for Developmental Neurobiology King | 1 | 0.82521 | 0.12349 | 0.78545 | 0.57446 | 76 | B | usable mapping rate | illumina | nextseq | 3prime | other | lexogen | bulk | unknown | unknown | United Kingdom | 2020-12-10 | Multi-stage | Multi-stage | Whole Organism | All anatomical structures | |||||||||||||||||||||
| 10145 | 10145 | ERR4973560 | ERX4792134 | ERS5459718 | ERP125703 | PRJEB41864 | three prime mRNA seq of sfpq / zebrafish embryos and siblings at 24 hpf | E-MTAB-9899 | Transcriptome Analysis | mRNA was purified from whole zebrafish embryos at 24 hpf and three prime proximal region was sequenced. | ENA FIRST PUBLIC:2021 01 27|ENA LAST UPDATE:2020 12 10 | Protocols: Embryos were collected from in crosses of sfpq+/ adult zebrafish RNA was extracted using the RNEasy Mini Kit Qiagen Library was generated using Lexogen's QuantSeq three prime mRNA Seq Library Prep Kit FWD for Illumina | sfpq 2 | SAMEA7703209 | Centre for Developmental Neurobiology King's College London | ENA first public:2021 01 27|ENA last update:2020 12 10|External Id:SAMEA7703209|INSDC center alias:Centre for Developmental Neurobiology King's College London|INSDC center name:Centre for Developmental Neurobiology King's College London|INSDC first public:2021 01 27T17:06:57Z|INSDC last update:2020 12 10T17:23:32Z|INSDC status:public|Submitter Id:E MTAB 9899:sfpq 2|age:24|broker name:ArrayExpress|common name:zebrafish|developmental stage:embryo stage|genotype:sfpq / |organism part:whole organism|sample name:E MTAB 9899:sfpq 2 | NextSeq 500 sequencing; 3 prime mRNA seq of sfpq / zebrafish embryos and siblings at 24 hpf | E MTAB 9899:sfpq 2 s | sfpq 2 s | three prime mRNA seq of sfpq / zebrafish embryos and siblings at 24 hpf | Embryos were collected from in crosses of sfpq+/ adult zebrafish RNA was extracted using the RNEasy Mini Kit Qiagen Library was generated using Lexogen's QuantSeq three prime mRNA Seq Library Prep Kit FWD for Illumina | Experimental Factor: genotype:sfpq / | RNA-Seq | TRANSCRIPTOMIC | RACE | SINGLE | ILLUMINA | NextSeq 500 | ERP125703 | NextSeq 500 sequencing; three prime mRNA seq of sfpq / zebrafish embryos and siblings at 24 hpf | ENA FIRST PUBLIC:2021 01 27|ENA LAST UPDATE:2020 12 10 | 66_GACGAT_66_TCAGTC_S18_R1_001.fastq.gz | fastq | E MTAB 9899:sfpq 2 | 0:76 1:0 | A:426637005;C:227027693;G:257342561;T:338159540;N:5909 | 76 | 0 | 426637005 | 227027693 | 257342561 | 338159540 | 5909 | ERX4792134 | ERS5459718 | ERA3194022 | Centre for Developmental Neurobiology King | Centre for Developmental Neurobiology King | 1 | 0.81933 | 0.11571 | 0.78593 | 0.56675 | 76 | B | usable mapping rate | illumina | nextseq | 3prime | other | lexogen | bulk | unknown | unknown | United Kingdom | 2020-12-10 | Multi-stage | Multi-stage | Whole Organism | All anatomical structures | |||||||||||||||||||||
| 10146 | 10146 | ERR4973559 | ERX4792133 | ERS5459717 | ERP125703 | PRJEB41864 | three prime mRNA seq of sfpq / zebrafish embryos and siblings at 24 hpf | E-MTAB-9899 | Transcriptome Analysis | mRNA was purified from whole zebrafish embryos at 24 hpf and three prime proximal region was sequenced. | ENA FIRST PUBLIC:2021 01 27|ENA LAST UPDATE:2020 12 10 | Protocols: Embryos were collected from in crosses of sfpq+/ adult zebrafish RNA was extracted using the RNEasy Mini Kit Qiagen Library was generated using Lexogen's QuantSeq three prime mRNA Seq Library Prep Kit FWD for Illumina | sfpq 1 | SAMEA7703208 | Centre for Developmental Neurobiology King's College London | ENA first public:2021 01 27|ENA last update:2020 12 10|External Id:SAMEA7703208|INSDC center alias:Centre for Developmental Neurobiology King's College London|INSDC center name:Centre for Developmental Neurobiology King's College London|INSDC first public:2021 01 27T17:06:57Z|INSDC last update:2020 12 10T17:23:32Z|INSDC status:public|Submitter Id:E MTAB 9899:sfpq 1|age:24|broker name:ArrayExpress|common name:zebrafish|developmental stage:embryo stage|genotype:sfpq / |organism part:whole organism|sample name:E MTAB 9899:sfpq 1 | NextSeq 500 sequencing; 3 prime mRNA seq of sfpq / zebrafish embryos and siblings at 24 hpf | E MTAB 9899:sfpq 1 s | sfpq 1 s | three prime mRNA seq of sfpq / zebrafish embryos and siblings at 24 hpf | Embryos were collected from in crosses of sfpq+/ adult zebrafish RNA was extracted using the RNEasy Mini Kit Qiagen Library was generated using Lexogen's QuantSeq three prime mRNA Seq Library Prep Kit FWD for Illumina | Experimental Factor: genotype:sfpq / | RNA-Seq | TRANSCRIPTOMIC | RACE | SINGLE | ILLUMINA | NextSeq 500 | ERP125703 | NextSeq 500 sequencing; three prime mRNA seq of sfpq / zebrafish embryos and siblings at 24 hpf | ENA FIRST PUBLIC:2021 01 27|ENA LAST UPDATE:2020 12 10 | 65_AAGCTC_65_AAGAAG_S3_R1_001.fastq.gz | fastq | E MTAB 9899:sfpq 1 | 0:76 1:0 | A:425613638;C:225707961;G:254738302;T:336863560;N:5847 | 76 | 0 | 425613638 | 225707961 | 254738302 | 336863560 | 5847 | ERX4792133 | ERS5459717 | ERA3194022 | Centre for Developmental Neurobiology King | Centre for Developmental Neurobiology King | 1 | 0.81857 | 0.11342 | 0.78587 | 0.56439 | 76 | B | usable mapping rate | illumina | nextseq | 3prime | other | lexogen | bulk | unknown | unknown | United Kingdom | 2020-12-10 | Multi-stage | Multi-stage | Whole Organism | All anatomical structures | |||||||||||||||||||||
| 40376 | 40376 | SRR3166964 | SRX1583817 | SRS1295580 | SRP070056 | PRJNA309588 | Analysis of the T cell response in Zebrafish | PRJNA309588 | Other | Our understanding of T cell receptor TCR repertoire diversity and response tochallenge is still incomplete. For example TCR clones shared by different individuals withminimal alteration to germline gene sequences public clones are detectable in all vertebrates but their significance is unknown. We exploited the experimental advantages offered by thezebrafish to analyze the complete TCR repertoire and its response to self and foreign antigens.We found that cross reactive public TCRs dominate the T cell response endowing the TCRrepertoire with the ability to cope with diverse antigenic challenges. These features of vertebratepublic TCRs provide a mechanism for the rapid generation of protective T cell immunityallowing a short temporal window for the development of more specific private T cell responses. | TCRA library 28d Calm4 | breed:AB|chain:alpha|index:26|sex:male|time point:28d|tissue:whole fish|age:1y|BioSampleModel:Model organism or animal | TCRa sequencing of zebrafish: whole zebrafish: Sample TCRA library 28d Calm4 | 116 28d Calm4 alpha | 116 28d Calm4 alpha | five prime RACE amplification of TCRa transcript | AMPLICON | TRANSCRIPTOMIC | RACE | PAIRED | ILLUMINA | Illumina HiSeq 2000 | <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>300</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>151</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> | SRP070056 | read1_116_28d_Calm4_alpha.fq.gz read2_116_28d_Calm4_alpha.fq.gz | fastq fastq | 12052440600.0 | 40174802.0 | 116 28d Calm4 alpha files | 0:150 1:150 | A:3274476247;C:2324807837;G:3578878507;T:2848354560;N:25923449 | 150 | 150 | 3274476247 | 2324807837 | 3578878507 | 2848354560 | 25923449 | SRX1583817 | SRS1295580 | SRA353254 | SRA | Bar-Ilan University | 2 | 0.00096 | 0.22917 | 0.00017 | 0.19822 | 0.99906 | 0.99762 | 0.35172 | 0.6189 | 150 | 150 | T | B | mate1 technical by mapping diff | illumina | hiseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | Israel | 2017-02-12 | Undetermined | Undetermined | Whole Organism | All anatomical structures | ||||||||||||||||||||
| 40377 | 40377 | SRR3166963 | SRX1583816 | SRS1295581 | SRP070056 | PRJNA309588 | Analysis of the T cell response in Zebrafish | PRJNA309588 | Other | Our understanding of T cell receptor TCR repertoire diversity and response tochallenge is still incomplete. For example TCR clones shared by different individuals withminimal alteration to germline gene sequences public clones are detectable in all vertebrates but their significance is unknown. We exploited the experimental advantages offered by thezebrafish to analyze the complete TCR repertoire and its response to self and foreign antigens.We found that cross reactive public TCRs dominate the T cell response endowing the TCRrepertoire with the ability to cope with diverse antigenic challenges. These features of vertebratepublic TCRs provide a mechanism for the rapid generation of protective T cell immunityallowing a short temporal window for the development of more specific private T cell responses. | TCRA library 28d Calm2 | breed:AB|chain:alpha|index:25|sex:male|time point:28d|tissue:whole fish|age:1y|BioSampleModel:Model organism or animal | TCRa sequencing of zebrafish: whole zebrafish: Sample TCRA library 28d Calm2 | 114 28d Calm2 alpha | 114 28d Calm2 alpha | five prime RACE amplification of TCRa transcript | AMPLICON | TRANSCRIPTOMIC | RACE | PAIRED | ILLUMINA | Illumina HiSeq 2000 | <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>300</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>151</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> | SRP070056 | read1_114_28d_Calm2_alpha.fq.gz read2_114_28d_Calm2_alpha.fq.gz | fastq fastq | 4957122900.0 | 16523743.0 | 114 28d Calm2 alpha files | 0:150 1:150 | A:1398510544;C:988027281;G:1244120221;T:1316350204;N:10114650 | 150 | 150 | 1398510544 | 988027281 | 1244120221 | 1316350204 | 10114650 | SRX1583816 | SRS1295581 | SRA353254 | SRA | Bar-Ilan University | 2 | 0.00167 | 0.03332 | 0.00146 | 0.028 | 0.99967 | 0.99896 | 0.15789 | 0.60731 | 150 | 150 | T | T | mates < 9% mapping rate | illumina | hiseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | Israel | 2017-02-12 | Undetermined | Undetermined | Whole Organism | All anatomical structures | ||||||||||||||||||||
| 40378 | 40378 | SRR3166962 | SRX1583815 | SRS1295582 | SRP070056 | PRJNA309588 | Analysis of the T cell response in Zebrafish | PRJNA309588 | Other | Our understanding of T cell receptor TCR repertoire diversity and response tochallenge is still incomplete. For example TCR clones shared by different individuals withminimal alteration to germline gene sequences public clones are detectable in all vertebrates but their significance is unknown. We exploited the experimental advantages offered by thezebrafish to analyze the complete TCR repertoire and its response to self and foreign antigens.We found that cross reactive public TCRs dominate the T cell response endowing the TCRrepertoire with the ability to cope with diverse antigenic challenges. These features of vertebratepublic TCRs provide a mechanism for the rapid generation of protective T cell immunityallowing a short temporal window for the development of more specific private T cell responses. | TCRA library 28d KLH6 | breed:AB|chain:alpha|index:21|sex:male|time point:28d|tissue:whole fish|age:1y|BioSampleModel:Model organism or animal | TCRa sequencing of zebrafish: whole zebrafish: Sample TCRA library 28d KLH6 | 110 28d KLH6 alpha | 110 28d KLH6 alpha | five prime RACE amplification of TCRa transcript | AMPLICON | TRANSCRIPTOMIC | RACE | PAIRED | ILLUMINA | Illumina HiSeq 2000 | <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>300</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>151</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> | SRP070056 | read1_110_28d_KLH6_alpha.fq.gz read2_110_28d_KLH6_alpha.fq.gz | fastq fastq | 6270530700.0 | 20901769.0 | 110 28d KLH6 alpha files | 0:150 1:150 | A:1802311183;C:1236560108;G:1561615962;T:1659163742;N:10879705 | 150 | 150 | 1802311183 | 1236560108 | 1561615962 | 1659163742 | 10879705 | SRX1583815 | SRS1295582 | SRA353254 | SRA | Bar-Ilan University | 2 | 0.00179 | 0.13512 | 0.00073 | 0.11592 | 0.99967 | 0.99855 | 0.21568 | 0.87835 | 150 | 150 | T | B | mate1 technical by mapping diff | illumina | hiseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | Israel | 2017-02-12 | Undetermined | Undetermined | Whole Organism | All anatomical structures | ||||||||||||||||||||
| 40379 | 40379 | SRR3166961 | SRX1583814 | SRS1295583 | SRP070056 | PRJNA309588 | Analysis of the T cell response in Zebrafish | PRJNA309588 | Other | Our understanding of T cell receptor TCR repertoire diversity and response tochallenge is still incomplete. For example TCR clones shared by different individuals withminimal alteration to germline gene sequences public clones are detectable in all vertebrates but their significance is unknown. We exploited the experimental advantages offered by thezebrafish to analyze the complete TCR repertoire and its response to self and foreign antigens.We found that cross reactive public TCRs dominate the T cell response endowing the TCRrepertoire with the ability to cope with diverse antigenic challenges. These features of vertebratepublic TCRs provide a mechanism for the rapid generation of protective T cell immunityallowing a short temporal window for the development of more specific private T cell responses. | TCRA library 28d PHA6 | breed:AB|chain:alpha|index:32|sex:male|time point:28d|tissue:whole fish|age:1y|BioSampleModel:Model organism or animal | TCRa sequencing of zebrafish: whole zebrafish: Sample TCRA library 28d PHA6 | 102 28d PHA6 alpha | 102 28d PHA6 alpha | five prime RACE amplification of TCRa transcript | AMPLICON | TRANSCRIPTOMIC | RACE | PAIRED | ILLUMINA | Illumina HiSeq 2000 | <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>300</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>151</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> | SRP070056 | read1_102_28d_PHA6_alpha.fq.gz read2_102_28d_PHA6_alpha.fq.gz | fastq fastq | 10067723700.0 | 33559079.0 | 102 28d PHA6 alpha files | 0:150 1:150 | A:2803515995;C:2027880463;G:2649055724;T:2566963260;N:20308258 | 150 | 150 | 2803515995 | 2027880463 | 2649055724 | 2566963260 | 20308258 | SRX1583814 | SRS1295583 | SRA353254 | SRA | Bar-Ilan University | 2 | 0.00158 | 0.04576 | 0.00056 | 0.03839 | 0.99922 | 0.99831 | 0.3246 | 0.40078 | 150 | 150 | T | T | mates < 9% mapping rate | illumina | hiseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | Israel | 2016-02-14 | Undetermined | Undetermined | Whole Organism | All anatomical structures | ||||||||||||||||||||
| 40380 | 40380 | SRR3166960 | SRX1583813 | SRS1295584 | SRP070056 | PRJNA309588 | Analysis of the T cell response in Zebrafish | PRJNA309588 | Other | Our understanding of T cell receptor TCR repertoire diversity and response tochallenge is still incomplete. For example TCR clones shared by different individuals withminimal alteration to germline gene sequences public clones are detectable in all vertebrates but their significance is unknown. We exploited the experimental advantages offered by thezebrafish to analyze the complete TCR repertoire and its response to self and foreign antigens.We found that cross reactive public TCRs dominate the T cell response endowing the TCRrepertoire with the ability to cope with diverse antigenic challenges. These features of vertebratepublic TCRs provide a mechanism for the rapid generation of protective T cell immunityallowing a short temporal window for the development of more specific private T cell responses. | TCRA library 7d KLH2 | breed:AB|chain:alpha|index:34|sex:male|time point:7d|tissue:whole fish|age:1y|BioSampleModel:Model organism or animal | TCRa sequencing of zebrafish: whole zebrafish: Sample TCRA library 7d KLH2 | 16 7d KLH2 alpha | 16 7d KLH2 alpha | five prime RACE amplification of TCRa transcript | AMPLICON | TRANSCRIPTOMIC | RACE | PAIRED | ILLUMINA | Illumina HiSeq 2000 | <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>300</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>151</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> | SRP070056 | read1_16_7d_KLH2_alpha.fq.gz read2_16_7d_KLH2_alpha.fq.gz | fastq fastq | 2783317500.0 | 9277725.0 | 16 7d KLH2 alpha files | 0:150 1:150 | A:761370556;C:556705024;G:719835894;T:727504605;N:17901421 | 150 | 150 | 761370556 | 556705024 | 719835894 | 727504605 | 17901421 | SRX1583813 | SRS1295584 | SRA353254 | SRA | Bar-Ilan University | 2 | 0.00143 | 0.13658 | 0.0008 | 0.11879 | 0.99935 | 0.99876 | 0.25409 | 0.87567 | 150 | 150 | T | B | mate1 technical by mapping diff | illumina | hiseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | Israel | 2016-02-14 | Undetermined | Undetermined | Whole Organism | All anatomical structures | ||||||||||||||||||||
| 40381 | 40381 | SRR3166959 | SRX1583812 | SRS1295585 | SRP070056 | PRJNA309588 | Analysis of the T cell response in Zebrafish | PRJNA309588 | Other | Our understanding of T cell receptor TCR repertoire diversity and response tochallenge is still incomplete. For example TCR clones shared by different individuals withminimal alteration to germline gene sequences public clones are detectable in all vertebrates but their significance is unknown. We exploited the experimental advantages offered by thezebrafish to analyze the complete TCR repertoire and its response to self and foreign antigens.We found that cross reactive public TCRs dominate the T cell response endowing the TCRrepertoire with the ability to cope with diverse antigenic challenges. These features of vertebratepublic TCRs provide a mechanism for the rapid generation of protective T cell immunityallowing a short temporal window for the development of more specific private T cell responses. | TCRA library 21d KLH6 | breed:AB|chain:alpha|index:24|sex:male|time point:21d|tissue:whole fish|age:1y|BioSampleModel:Model organism or animal | TCRa sequencing of zebrafish: whole zebrafish: Sample TCRA library 21d KLH6 | 78 21d KLH6 alpha | 78 21d KLH6 alpha | five prime RACE amplification of TCRa transcript | AMPLICON | TRANSCRIPTOMIC | RACE | PAIRED | ILLUMINA | Illumina HiSeq 2000 | <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>300</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>151</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> | SRP070056 | read1_78_21d_KLH6_alpha.fq.gz read2_78_21d_KLH6_alpha.fq.gz | fastq fastq | 7720614900.0 | 25735383.0 | 78 21d KLH6 alpha files | 0:150 1:150 | A:2163229607;C:1591913203;G:1963558161;T:1998596363;N:3317566 | 150 | 150 | 2163229607 | 1591913203 | 1963558161 | 1998596363 | 3317566 | SRX1583812 | SRS1295585 | SRA353254 | SRA | Bar-Ilan University | 2 | 0.00105 | 0.04913 | 0.00036 | 0.04186 | 0.99937 | 0.99835 | 0.14393 | 0.4819 | 150 | 150 | T | T | mates < 9% mapping rate | illumina | hiseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | Israel | 2017-02-12 | Undetermined | Undetermined | Whole Organism | All anatomical structures | ||||||||||||||||||||
| 40382 | 40382 | SRR3166958 | SRX1583811 | SRS1295586 | SRP070056 | PRJNA309588 | Analysis of the T cell response in Zebrafish | PRJNA309588 | Other | Our understanding of T cell receptor TCR repertoire diversity and response tochallenge is still incomplete. For example TCR clones shared by different individuals withminimal alteration to germline gene sequences public clones are detectable in all vertebrates but their significance is unknown. We exploited the experimental advantages offered by thezebrafish to analyze the complete TCR repertoire and its response to self and foreign antigens.We found that cross reactive public TCRs dominate the T cell response endowing the TCRrepertoire with the ability to cope with diverse antigenic challenges. These features of vertebratepublic TCRs provide a mechanism for the rapid generation of protective T cell immunityallowing a short temporal window for the development of more specific private T cell responses. | TCRA library 7d Calm6 | breed:AB|chain:alpha|index:33|sex:male|time point:7d|tissue:whole fish|age:1y|BioSampleModel:Model organism or animal | TCRa sequencing of zebrafish: whole zebrafish: Sample TCRA library 7d Calm6 | 27 7d Calm6 alpha | 27 7d Calm6 alpha | five prime RACE amplification of TCRa transcript | AMPLICON | TRANSCRIPTOMIC | RACE | PAIRED | ILLUMINA | Illumina HiSeq 2000 | <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>300</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>151</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> | SRP070056 | read1_27_7d_Calm6_alpha.fq.gz read2_27_7d_Calm6_alpha.fq.gz | fastq fastq | 4337226600.0 | 14457422.0 | 27 7d Calm6 alpha files | 0:150 1:150 | A:1174401516;C:871068073;G:1145864920;T:1118174763;N:27717328 | 150 | 150 | 1174401516 | 871068073 | 1145864920 | 1118174763 | 27717328 | SRX1583811 | SRS1295586 | SRA353254 | SRA | Bar-Ilan University | 2 | 0.002 | 0.032 | 0.0007 | 0.02494 | 0.99924 | 0.99912 | 0.38 | 0.38606 | 150 | 150 | T | T | mates < 9% mapping rate | illumina | hiseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | Israel | 2016-02-14 | Undetermined | Undetermined | Whole Organism | All anatomical structures | ||||||||||||||||||||
| 40383 | 40383 | SRR3166957 | SRX1583810 | SRS1295587 | SRP070056 | PRJNA309588 | Analysis of the T cell response in Zebrafish | PRJNA309588 | Other | Our understanding of T cell receptor TCR repertoire diversity and response tochallenge is still incomplete. For example TCR clones shared by different individuals withminimal alteration to germline gene sequences public clones are detectable in all vertebrates but their significance is unknown. We exploited the experimental advantages offered by thezebrafish to analyze the complete TCR repertoire and its response to self and foreign antigens.We found that cross reactive public TCRs dominate the T cell response endowing the TCRrepertoire with the ability to cope with diverse antigenic challenges. These features of vertebratepublic TCRs provide a mechanism for the rapid generation of protective T cell immunityallowing a short temporal window for the development of more specific private T cell responses. | TCRA library 7d PHA7 | breed:AB|chain:alpha|index:36|sex:male|time point:7d|tissue:whole fish|age:1y|BioSampleModel:Model organism or animal | TCRa sequencing of zebrafish: whole zebrafish: Sample TCRA library 7d PHA7 | 14 7d PHA7 alpha | 14 7d PHA7 alpha | five prime RACE amplification of TCRa transcript | AMPLICON | TRANSCRIPTOMIC | RACE | PAIRED | ILLUMINA | Illumina HiSeq 2000 | <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>300</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>151</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> | SRP070056 | read1_14_7d_PHA7_alpha.fq.gz read2_14_7d_PHA7_alpha.fq.gz | fastq fastq | 3401366100.0 | 11337887.0 | 14 7d PHA7 alpha files | SRX1583810 | SRS1295587 | SRA353254 | SRA | Bar-Ilan University | 2 | 0.00357 | 0.02242 | 0.00202 | 0.01952 | 0.99902 | 0.99811 | 0.35016 | 0.5424 | 150 | 150 | T | T | mates < 9% mapping rate | illumina | hiseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | Israel | 2017-02-12 | Undetermined | Undetermined | Whole Organism | All anatomical structures | |||||||||||||||||||||||||||||
| 40384 | 40384 | SRR3166956 | SRX1583809 | SRS1295588 | SRP070056 | PRJNA309588 | Analysis of the T cell response in Zebrafish | PRJNA309588 | Other | Our understanding of T cell receptor TCR repertoire diversity and response tochallenge is still incomplete. For example TCR clones shared by different individuals withminimal alteration to germline gene sequences public clones are detectable in all vertebrates but their significance is unknown. We exploited the experimental advantages offered by thezebrafish to analyze the complete TCR repertoire and its response to self and foreign antigens.We found that cross reactive public TCRs dominate the T cell response endowing the TCRrepertoire with the ability to cope with diverse antigenic challenges. These features of vertebratepublic TCRs provide a mechanism for the rapid generation of protective T cell immunityallowing a short temporal window for the development of more specific private T cell responses. | TCRA library 7d PHA3 | breed:AB|chain:alpha|index:35|sex:male|time point:7d|tissue:whole fish|age:1y|BioSampleModel:Model organism or animal | TCRa sequencing of zebrafish: whole zebrafish: Sample TCRA library 7d PHA3 | 10 7d PHA3 alpha | 10 7d PHA3 alpha | five prime RACE amplification of TCRa transcript | AMPLICON | TRANSCRIPTOMIC | RACE | PAIRED | ILLUMINA | Illumina HiSeq 2000 | <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>300</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>151</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> | SRP070056 | read2_10_7d_PHA3_alpha.fq.gz read1_10_7d_PHA3_alpha.fq.gz | fastq fastq | 4293459300.0 | 14311531.0 | 10 7d PHA3 alpha files | 0:150 1:150 | A:1208054755;C:855800391;G:1072212534;T:1129932131;N:27459489 | 150 | 150 | 1208054755 | 855800391 | 1072212534 | 1129932131 | 27459489 | SRX1583809 | SRS1295588 | SRA353254 | SRA | Bar-Ilan University | 2 | 0.00174 | 0.05155 | 0.0009 | 0.0472 | 0.99937 | 0.9989 | 0.2256 | 0.60714 | 150 | 150 | T | T | mates < 9% mapping rate | illumina | hiseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | Israel | 2017-02-12 | Undetermined | Undetermined | Whole Organism | All anatomical structures | ||||||||||||||||||||
| 40385 | 40385 | SRR3166955 | SRX1583808 | SRS1295589 | SRP070056 | PRJNA309588 | Analysis of the T cell response in Zebrafish | PRJNA309588 | Other | Our understanding of T cell receptor TCR repertoire diversity and response tochallenge is still incomplete. For example TCR clones shared by different individuals withminimal alteration to germline gene sequences public clones are detectable in all vertebrates but their significance is unknown. We exploited the experimental advantages offered by thezebrafish to analyze the complete TCR repertoire and its response to self and foreign antigens.We found that cross reactive public TCRs dominate the T cell response endowing the TCRrepertoire with the ability to cope with diverse antigenic challenges. These features of vertebratepublic TCRs provide a mechanism for the rapid generation of protective T cell immunityallowing a short temporal window for the development of more specific private T cell responses. | TCRB library 28d Calm7 | breed:AB|chain:beta|index:56|sex:male|time point:28d|tissue:whole fish|age:1y|BioSampleModel:Model organism or animal | TCRb sequencing of zebrafish: whole zebrafish: Sample TCRB library 28d Calm7 | 119 28d Calm7 beta | 119 28d Calm7 beta | five prime RACE amplification of TCRb transcript | AMPLICON | TRANSCRIPTOMIC | RACE | PAIRED | ILLUMINA | Illumina HiSeq 2000 | <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>300</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>151</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> | SRP070056 | read1_119_28d_Calm7_beta.fq.gz read2_119_28d_Calm7_beta.fq.gz | fastq fastq | 3494941800.0 | 11649806.0 | 119 28d Calm7 beta files | 0:150 1:150 | A:1262121526;C:602497563;G:790544892;T:738346792;N:101431027 | 150 | 150 | 1262121526 | 602497563 | 790544892 | 738346792 | 101431027 | SRX1583808 | SRS1295589 | SRA353254 | SRA | Bar-Ilan University | 2 | 3e-05 | 0.00179 | 0.0 | 7e-05 | 0.99997 | 0.99831 | 0.0 | 0.42009 | 150 | 150 | T | T | mates < 9% mapping rate | illumina | hiseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | Israel | 2017-02-12 | Undetermined | Undetermined | Whole Organism | All anatomical structures | ||||||||||||||||||||
| 40386 | 40386 | SRR3166954 | SRX1583807 | SRS1295590 | SRP070056 | PRJNA309588 | Analysis of the T cell response in Zebrafish | PRJNA309588 | Other | Our understanding of T cell receptor TCR repertoire diversity and response tochallenge is still incomplete. For example TCR clones shared by different individuals withminimal alteration to germline gene sequences public clones are detectable in all vertebrates but their significance is unknown. We exploited the experimental advantages offered by thezebrafish to analyze the complete TCR repertoire and its response to self and foreign antigens.We found that cross reactive public TCRs dominate the T cell response endowing the TCRrepertoire with the ability to cope with diverse antigenic challenges. These features of vertebratepublic TCRs provide a mechanism for the rapid generation of protective T cell immunityallowing a short temporal window for the development of more specific private T cell responses. | TCRB library 28d Calm5 | breed:AB|chain:beta|index:55|sex:male|time point:28d|tissue:whole fish|age:1y|BioSampleModel:Model organism or animal | TCRb sequencing of zebrafish: whole zebrafish: Sample TCRB library 28d Calm5 | 117 28d Calm5 beta | 117 28d Calm5 beta | five prime RACE amplification of TCRb transcript | AMPLICON | TRANSCRIPTOMIC | RACE | PAIRED | ILLUMINA | Illumina HiSeq 2000 | <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>300</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>151</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> | SRP070056 | read2_117_28d_Calm5_beta.fq.gz read1_117_28d_Calm5_beta.fq.gz | fastq fastq | 2950876200.0 | 9836254.0 | 117 28d Calm5 beta files | 0:150 1:150 | A:1025845698;C:505599350;G:688977765;T:634738210;N:95715177 | 150 | 150 | 1025845698 | 505599350 | 688977765 | 634738210 | 95715177 | SRX1583807 | SRS1295590 | SRA353254 | SRA | Bar-Ilan University | 2 | 0.0 | 0.00023 | 0.0 | 0.0 | 1.0 | 0.99987 | 0.07692 | 150 | 150 | T | T | mates < 9% mapping rate | illumina | hiseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | Israel | 2016-03-02 | Undetermined | Undetermined | Whole Organism | All anatomical structures | |||||||||||||||||||||
| 40387 | 40387 | SRR3166953 | SRX1583806 | SRS1295591 | SRP070056 | PRJNA309588 | Analysis of the T cell response in Zebrafish | PRJNA309588 | Other | Our understanding of T cell receptor TCR repertoire diversity and response tochallenge is still incomplete. For example TCR clones shared by different individuals withminimal alteration to germline gene sequences public clones are detectable in all vertebrates but their significance is unknown. We exploited the experimental advantages offered by thezebrafish to analyze the complete TCR repertoire and its response to self and foreign antigens.We found that cross reactive public TCRs dominate the T cell response endowing the TCRrepertoire with the ability to cope with diverse antigenic challenges. These features of vertebratepublic TCRs provide a mechanism for the rapid generation of protective T cell immunityallowing a short temporal window for the development of more specific private T cell responses. | TCRB library 28d Calm3 | breed:AB|chain:beta|index:54|sex:male|time point:28d|tissue:whole fish|age:1y|BioSampleModel:Model organism or animal | TCRb sequencing of zebrafish: whole zebrafish: Sample TCRB library 28d Calm3 | 115 28d Calm3 beta | 115 28d Calm3 beta | five prime RACE amplification of TCRb transcript | AMPLICON | TRANSCRIPTOMIC | RACE | PAIRED | ILLUMINA | Illumina HiSeq 2000 | <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>300</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>151</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> | SRP070056 | read1_115_28d_Calm3_beta.fq.gz read2_115_28d_Calm3_beta.fq.gz | fastq fastq | 1913218200.0 | 6377394.0 | 115 28d Calm3 beta files | 0:150 1:150 | A:637646003;C:349779874;G:435732113;T:430619528;N:59440682 | 150 | 150 | 637646003 | 349779874 | 435732113 | 430619528 | 59440682 | SRX1583806 | SRS1295591 | SRA353254 | SRA | Bar-Ilan University | 2 | 0.0 | 0.02438 | 0.0 | 0.00184 | 1.0 | 0.99908 | 0.01511 | 150 | 150 | T | T | mates < 9% mapping rate | illumina | hiseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | Israel | 2017-02-12 | Undetermined | Undetermined | Whole Organism | All anatomical structures | |||||||||||||||||||||
| 40388 | 40388 | SRR3166952 | SRX1583805 | SRS1295592 | SRP070056 | PRJNA309588 | Analysis of the T cell response in Zebrafish | PRJNA309588 | Other | Our understanding of T cell receptor TCR repertoire diversity and response tochallenge is still incomplete. For example TCR clones shared by different individuals withminimal alteration to germline gene sequences public clones are detectable in all vertebrates but their significance is unknown. We exploited the experimental advantages offered by thezebrafish to analyze the complete TCR repertoire and its response to self and foreign antigens.We found that cross reactive public TCRs dominate the T cell response endowing the TCRrepertoire with the ability to cope with diverse antigenic challenges. These features of vertebratepublic TCRs provide a mechanism for the rapid generation of protective T cell immunityallowing a short temporal window for the development of more specific private T cell responses. | TCRB library 28d KLH6 | breed:AB|chain:beta|index:27|sex:male|time point:28d|tissue:whole fish|age:1y|BioSampleModel:Model organism or animal | TCRb sequencing of zebrafish: whole zebrafish: Sample TCRB library 28d KLH6 | 110 28d KLH6 beta | 110 28d KLH6 beta | five prime RACE amplification of TCRb transcript | AMPLICON | TRANSCRIPTOMIC | RACE | PAIRED | ILLUMINA | Illumina HiSeq 2000 | <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>300</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>151</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> | SRP070056 | read1_110_28d_KLH6_beta.fq.gz read2_110_28d_KLH6_beta.fq.gz | fastq fastq | 2689206600.0 | 8964022.0 | 110 28d KLH6 beta files | 0:150 1:150 | A:807762783;C:503825062;G:585283183;T:703818890;N:88516682 | 150 | 150 | 807762783 | 503825062 | 585283183 | 703818890 | 88516682 | SRX1583805 | SRS1295592 | SRA353254 | SRA | Bar-Ilan University | 2 | 0.00014 | 0.3078 | 0.0 | 0.02202 | 0.99975 | 0.99095 | 0.38888 | 0.10297 | 150 | 150 | T | B | mate1 technical by mapping diff | illumina | hiseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | Israel | 2017-02-12 | Undetermined | Undetermined | Whole Organism | All anatomical structures | ||||||||||||||||||||
| 40389 | 40389 | SRR3166951 | SRX1583804 | SRS1295593 | SRP070056 | PRJNA309588 | Analysis of the T cell response in Zebrafish | PRJNA309588 | Other | Our understanding of T cell receptor TCR repertoire diversity and response tochallenge is still incomplete. For example TCR clones shared by different individuals withminimal alteration to germline gene sequences public clones are detectable in all vertebrates but their significance is unknown. We exploited the experimental advantages offered by thezebrafish to analyze the complete TCR repertoire and its response to self and foreign antigens.We found that cross reactive public TCRs dominate the T cell response endowing the TCRrepertoire with the ability to cope with diverse antigenic challenges. These features of vertebratepublic TCRs provide a mechanism for the rapid generation of protective T cell immunityallowing a short temporal window for the development of more specific private T cell responses. | TCRB library 28d KLH2 | breed:AB|chain:beta|index:23|sex:male|time point:28d|tissue:whole fish|age:1y|BioSampleModel:Model organism or animal | TCRb sequencing of zebrafish: whole zebrafish: Sample TCRB library 28d KLH2 | 106 28d KLH2 beta | 106 28d KLH2 beta | five prime RACE amplification of TCRb transcript | AMPLICON | TRANSCRIPTOMIC | RACE | PAIRED | ILLUMINA | Illumina HiSeq 2000 | <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>300</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>151</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> | SRP070056 | read2_106_28d_KLH2_beta.fq.gz read1_106_28d_KLH2_beta.fq.gz | fastq fastq | 2795086200.0 | 9316954.0 | 106 28d KLH2 beta files | 0:150 1:150 | A:897420089;C:470460508;G:621809014;T:712999451;N:92397138 | 150 | 150 | 897420089 | 470460508 | 621809014 | 712999451 | 92397138 | SRX1583804 | SRS1295593 | SRA353254 | SRA | Bar-Ilan University | 2 | 0.0 | 0.05106 | 0.0 | 0.00859 | 1.0 | 0.99943 | 0.01388 | 150 | 150 | T | T | mates < 9% mapping rate | illumina | hiseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | Israel | 2016-03-02 | Undetermined | Undetermined | Whole Organism | All anatomical structures | |||||||||||||||||||||
| 40390 | 40390 | SRR3166950 | SRX1583803 | SRS1295594 | SRP070056 | PRJNA309588 | Analysis of the T cell response in Zebrafish | PRJNA309588 | Other | Our understanding of T cell receptor TCR repertoire diversity and response tochallenge is still incomplete. For example TCR clones shared by different individuals withminimal alteration to germline gene sequences public clones are detectable in all vertebrates but their significance is unknown. We exploited the experimental advantages offered by thezebrafish to analyze the complete TCR repertoire and its response to self and foreign antigens.We found that cross reactive public TCRs dominate the T cell response endowing the TCRrepertoire with the ability to cope with diverse antigenic challenges. These features of vertebratepublic TCRs provide a mechanism for the rapid generation of protective T cell immunityallowing a short temporal window for the development of more specific private T cell responses. | TCRB library 28d KLH1 | breed:AB|chain:beta|index:22|sex:male|time point:28d|tissue:whole fish|age:1y|BioSampleModel:Model organism or animal | TCRb sequencing of zebrafish: whole zebrafish: Sample TCRB library 28d KLH1 | 105 28d KLH1 beta | 105 28d KLH1 beta | five prime RACE amplification of TCRb transcript | AMPLICON | TRANSCRIPTOMIC | RACE | PAIRED | ILLUMINA | Illumina HiSeq 2000 | <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>300</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>151</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> | SRP070056 | read1_105_28d_KLH1_beta.fq.gz read2_105_28d_KLH1_beta.fq.gz | fastq fastq | 1768628700.0 | 5895429.0 | 105 28d KLH1 beta files | 0:150 1:150 | A:486366963;C:366398128;G:389871583;T:478235213;N:47756813 | 150 | 150 | 486366963 | 366398128 | 389871583 | 478235213 | 47756813 | SRX1583803 | SRS1295594 | SRA353254 | SRA | Bar-Ilan University | 2 | 4e-05 | 0.09054 | 3e-05 | 0.01597 | 1.0 | 0.99931 | 0.00767 | 150 | 150 | T | B | mate1 technical by mapping diff | illumina | hiseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | Israel | 2017-02-12 | Undetermined | Undetermined | Whole Organism | All anatomical structures | |||||||||||||||||||||
| 40391 | 40391 | SRR3166949 | SRX1583802 | SRS1295595 | SRP070056 | PRJNA309588 | Analysis of the T cell response in Zebrafish | PRJNA309588 | Other | Our understanding of T cell receptor TCR repertoire diversity and response tochallenge is still incomplete. For example TCR clones shared by different individuals withminimal alteration to germline gene sequences public clones are detectable in all vertebrates but their significance is unknown. We exploited the experimental advantages offered by thezebrafish to analyze the complete TCR repertoire and its response to self and foreign antigens.We found that cross reactive public TCRs dominate the T cell response endowing the TCRrepertoire with the ability to cope with diverse antigenic challenges. These features of vertebratepublic TCRs provide a mechanism for the rapid generation of protective T cell immunityallowing a short temporal window for the development of more specific private T cell responses. | TCRB library 28d PHA7 | breed:AB|chain:beta|index:30|sex:male|time point:28d|tissue:whole fish|age:1y|BioSampleModel:Model organism or animal | TCRb sequencing of zebrafish: whole zebrafish: Sample TCRB library 28d PHA7 | 103 28d PHA7 beta | 103 28d PHA7 beta | five prime RACE amplification of TCRb transcript | AMPLICON | TRANSCRIPTOMIC | RACE | PAIRED | ILLUMINA | Illumina HiSeq 2000 | <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>300</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>151</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> | SRP070056 | read1_103_28d_PHA7_beta.fq.gz read2_103_28d_PHA7_beta.fq.gz | fastq fastq | 2234344500.0 | 7447815.0 | 103 28d PHA7 beta files | 0:150 1:150 | A:628358242;C:462371909;G:526170885;T:548473987;N:68969477 | 150 | 150 | 628358242 | 462371909 | 526170885 | 548473987 | 68969477 | SRX1583802 | SRS1295595 | SRA353254 | SRA | Bar-Ilan University | 2 | 0.00104 | 0.68691 | 5e-05 | 0.01999 | 0.99894 | 0.97281 | 0.23076 | 0.44172 | 150 | 150 | T | B | mate1 technical by mapping diff | illumina | hiseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | Israel | 2016-03-02 | Undetermined | Undetermined | Whole Organism | All anatomical structures | ||||||||||||||||||||
| 40392 | 40392 | SRR3166948 | SRX1583801 | SRS1295596 | SRP070056 | PRJNA309588 | Analysis of the T cell response in Zebrafish | PRJNA309588 | Other | Our understanding of T cell receptor TCR repertoire diversity and response tochallenge is still incomplete. For example TCR clones shared by different individuals withminimal alteration to germline gene sequences public clones are detectable in all vertebrates but their significance is unknown. We exploited the experimental advantages offered by thezebrafish to analyze the complete TCR repertoire and its response to self and foreign antigens.We found that cross reactive public TCRs dominate the T cell response endowing the TCRrepertoire with the ability to cope with diverse antigenic challenges. These features of vertebratepublic TCRs provide a mechanism for the rapid generation of protective T cell immunityallowing a short temporal window for the development of more specific private T cell responses. | TCRB library 28d PHA5 | breed:AB|chain:beta|index:29|sex:male|time point:28d|tissue:whole fish|age:1y|BioSampleModel:Model organism or animal | TCRb sequencing of zebrafish: whole zebrafish: Sample TCRB library 28d PHA5 | 101 28d PHA5 beta | 101 28d PHA5 beta | five prime RACE amplification of TCRb transcript | AMPLICON | TRANSCRIPTOMIC | RACE | PAIRED | ILLUMINA | Illumina HiSeq 2000 | <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>300</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>151</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> | SRP070056 | read1_101_28d_PHA5_beta.fq.gz read2_101_28d_PHA5_beta.fq.gz | fastq fastq | 1356278400.0 | 4520928.0 | 101 28d PHA5 beta files | 0:150 1:150 | A:372190640;C:278464953;G:294268430;T:378226253;N:33128124 | 150 | 150 | 372190640 | 278464953 | 294268430 | 378226253 | 33128124 | SRX1583801 | SRS1295596 | SRA353254 | SRA | Bar-Ilan University | 2 | 0.00254 | 0.10913 | 0.0001 | 0.01715 | 0.99995 | 0.99904 | 0.01106 | 0.01084 | 150 | 150 | T | B | mate1 technical by mapping diff | illumina | hiseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | Israel | 2017-02-12 | Undetermined | Undetermined | Whole Organism | All anatomical structures | ||||||||||||||||||||
| 40393 | 40393 | SRR3166947 | SRX1583800 | SRS1295597 | SRP070056 | PRJNA309588 | Analysis of the T cell response in Zebrafish | PRJNA309588 | Other | Our understanding of T cell receptor TCR repertoire diversity and response tochallenge is still incomplete. For example TCR clones shared by different individuals withminimal alteration to germline gene sequences public clones are detectable in all vertebrates but their significance is unknown. We exploited the experimental advantages offered by thezebrafish to analyze the complete TCR repertoire and its response to self and foreign antigens.We found that cross reactive public TCRs dominate the T cell response endowing the TCRrepertoire with the ability to cope with diverse antigenic challenges. These features of vertebratepublic TCRs provide a mechanism for the rapid generation of protective T cell immunityallowing a short temporal window for the development of more specific private T cell responses. | TCRB library 28d PHA2 | breed:AB|chain:beta|index:28|sex:male|time point:28d|tissue:whole fish|age:1y|BioSampleModel:Model organism or animal | TCRb sequencing of zebrafish: whole zebrafish: Sample TCRB library 28d PHA2 | 98 28d PHA2 beta | 98 28d PHA2 beta | five prime RACE amplification of TCRb transcript | AMPLICON | TRANSCRIPTOMIC | RACE | PAIRED | ILLUMINA | Illumina HiSeq 2000 | <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>300</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>151</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> | SRP070056 | read2_98_28d_PHA2_beta.fq.gz read1_98_28d_PHA2_beta.fq.gz | fastq fastq | 4533755400.0 | 15112518.0 | 98 28d PHA2 beta files | 0:150 1:150 | A:1508096116;C:749853448;G:1051562277;T:1142392486;N:81851073 | 150 | 150 | 1508096116 | 749853448 | 1051562277 | 1142392486 | 81851073 | SRX1583800 | SRS1295597 | SRA353254 | SRA | Bar-Ilan University | 2 | 0.0 | 0.33493 | 0.0 | 0.02388 | 1.0 | 0.99758 | 0.15819 | 150 | 150 | T | B | mate1 technical by mapping diff | illumina | hiseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | Israel | 2017-02-12 | Undetermined | Undetermined | Whole Organism | All anatomical structures | |||||||||||||||||||||
| 40394 | 40394 | SRR3166946 | SRX1583799 | SRS1295598 | SRP070056 | PRJNA309588 | Analysis of the T cell response in Zebrafish | PRJNA309588 | Other | Our understanding of T cell receptor TCR repertoire diversity and response tochallenge is still incomplete. For example TCR clones shared by different individuals withminimal alteration to germline gene sequences public clones are detectable in all vertebrates but their significance is unknown. We exploited the experimental advantages offered by thezebrafish to analyze the complete TCR repertoire and its response to self and foreign antigens.We found that cross reactive public TCRs dominate the T cell response endowing the TCRrepertoire with the ability to cope with diverse antigenic challenges. These features of vertebratepublic TCRs provide a mechanism for the rapid generation of protective T cell immunityallowing a short temporal window for the development of more specific private T cell responses. | TCRB library 28d IFA 5 | breed:AB|chain:beta|index:11|sex:male|time point:28d|tissue:whole fish|age:1y|BioSampleModel:Model organism or animal | TCRb sequencing of zebrafish: whole zebrafish: Sample TCRB library 28d IFA 5 | 93 28d IFA 5 beta | 93 28d IFA 5 beta | five prime RACE amplification of TCRb transcript | AMPLICON | TRANSCRIPTOMIC | RACE | PAIRED | ILLUMINA | Illumina HiSeq 2000 | <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>300</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>151</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> | SRP070056 | read1_93_28d_IFA_5_beta.fq.gz read2_93_28d_IFA_5_beta.fq.gz | fastq fastq | 1526394600.0 | 5087982.0 | 93 28d IFA 5 beta files | 0:150 1:150 | A:431910708;C:323148774;G:334806769;T:417586287;N:18942062 | 150 | 150 | 431910708 | 323148774 | 334806769 | 417586287 | 18942062 | SRX1583799 | SRS1295598 | SRA353254 | SRA | Bar-Ilan University | 2 | 0.00039 | 0.31457 | 0.00015 | 0.00771 | 0.99963 | 0.98468 | 0.28947 | 0.16209 | 150 | 150 | T | B | mate1 technical by mapping diff | illumina | hiseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | Israel | 2017-02-12 | Undetermined | Undetermined | Whole Organism | All anatomical structures | ||||||||||||||||||||
| 40395 | 40395 | SRR3166945 | SRX1583798 | SRS1295599 | SRP070056 | PRJNA309588 | Analysis of the T cell response in Zebrafish | PRJNA309588 | Other | Our understanding of T cell receptor TCR repertoire diversity and response tochallenge is still incomplete. For example TCR clones shared by different individuals withminimal alteration to germline gene sequences public clones are detectable in all vertebrates but their significance is unknown. We exploited the experimental advantages offered by thezebrafish to analyze the complete TCR repertoire and its response to self and foreign antigens.We found that cross reactive public TCRs dominate the T cell response endowing the TCRrepertoire with the ability to cope with diverse antigenic challenges. These features of vertebratepublic TCRs provide a mechanism for the rapid generation of protective T cell immunityallowing a short temporal window for the development of more specific private T cell responses. | TCRB library 28d IFA 2 | breed:AB|chain:beta|index:10|sex:male|time point:28d|tissue:whole fish|age:1y|BioSampleModel:Model organism or animal | TCRb sequencing of zebrafish: whole zebrafish: Sample TCRB library 28d IFA 2 | 90 28d IFA 2 beta | 90 28d IFA 2 beta | five prime RACE amplification of TCRb transcript | AMPLICON | TRANSCRIPTOMIC | RACE | PAIRED | ILLUMINA | Illumina HiSeq 2000 | <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>300</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>151</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> | SRP070056 | read1_90_28d_IFA_2_beta.fq.gz read2_90_28d_IFA_2_beta.fq.gz | fastq fastq | 2345298300.0 | 7817661.0 | 90 28d IFA 2 beta files | 0:150 1:150 | A:753823005;C:419081900;G:516233583;T:613533931;N:42625881 | 150 | 150 | 753823005 | 419081900 | 516233583 | 613533931 | 42625881 | SRX1583798 | SRS1295599 | SRA353254 | SRA | Bar-Ilan University | 2 | 0.00015 | 0.38592 | 3e-05 | 0.00508 | 0.99985 | 0.99385 | 0.33333 | 0.06202 | 150 | 150 | T | B | mate1 technical by mapping diff | illumina | hiseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | Israel | 2017-02-12 | Undetermined | Undetermined | Whole Organism | All anatomical structures | ||||||||||||||||||||
| 40396 | 40396 | SRR3166944 | SRX1583797 | SRS1295600 | SRP070056 | PRJNA309588 | Analysis of the T cell response in Zebrafish | PRJNA309588 | Other | Our understanding of T cell receptor TCR repertoire diversity and response tochallenge is still incomplete. For example TCR clones shared by different individuals withminimal alteration to germline gene sequences public clones are detectable in all vertebrates but their significance is unknown. We exploited the experimental advantages offered by thezebrafish to analyze the complete TCR repertoire and its response to self and foreign antigens.We found that cross reactive public TCRs dominate the T cell response endowing the TCRrepertoire with the ability to cope with diverse antigenic challenges. These features of vertebratepublic TCRs provide a mechanism for the rapid generation of protective T cell immunityallowing a short temporal window for the development of more specific private T cell responses. | TCRB library 28d IFA 1 | breed:AB|chain:beta|index:28|sex:male|time point:28d|tissue:whole fish|age:1y|BioSampleModel:Model organism or animal | TCRb sequencing of zebrafish: whole zebrafish: Sample TCRB library 28d IFA 1 | 89 28d IFA 1 beta | 89 28d IFA 1 beta | five prime RACE amplification of TCRb transcript | AMPLICON | TRANSCRIPTOMIC | RACE | PAIRED | ILLUMINA | Illumina HiSeq 2000 | <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>300</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>151</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> | SRP070056 | read1_89_28d_IFA_1_beta.fq.gz read2_89_28d_IFA_1_beta.fq.gz | fastq fastq | 2029349400.0 | 6764498.0 | 89 28d IFA 1 beta files | 0:150 1:150 | A:714287901;C:360307311;G:472841677;T:446872160;N:35040351 | 150 | 150 | 714287901 | 360307311 | 472841677 | 446872160 | 35040351 | SRX1583797 | SRS1295600 | SRA353254 | SRA | Bar-Ilan University | 2 | 0.0 | 0.06313 | 0.0 | 0.01031 | 1.0 | 0.99703 | 0.15591 | 150 | 150 | T | T | mates < 9% mapping rate | illumina | hiseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | Israel | 2017-02-12 | Undetermined | Undetermined | Whole Organism | All anatomical structures | |||||||||||||||||||||
| 40397 | 40397 | SRR3166943 | SRX1583796 | SRS1295601 | SRP070056 | PRJNA309588 | Analysis of the T cell response in Zebrafish | PRJNA309588 | Other | Our understanding of T cell receptor TCR repertoire diversity and response tochallenge is still incomplete. For example TCR clones shared by different individuals withminimal alteration to germline gene sequences public clones are detectable in all vertebrates but their significance is unknown. We exploited the experimental advantages offered by thezebrafish to analyze the complete TCR repertoire and its response to self and foreign antigens.We found that cross reactive public TCRs dominate the T cell response endowing the TCRrepertoire with the ability to cope with diverse antigenic challenges. These features of vertebratepublic TCRs provide a mechanism for the rapid generation of protective T cell immunityallowing a short temporal window for the development of more specific private T cell responses. | TCRB library 21d Calm6 | breed:AB|chain:beta|index:44|sex:male|time point:21d|tissue:whole fish|age:1y|BioSampleModel:Model organism or animal | TCRb sequencing of zebrafish: whole zebrafish: Sample TCRB library 21d Calm6 | 86 21d Calm6 beta | 86 21d Calm6 beta | five prime RACE amplification of TCRb transcript | AMPLICON | TRANSCRIPTOMIC | RACE | PAIRED | ILLUMINA | Illumina HiSeq 2000 | <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>300</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>151</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> | SRP070056 | read2_86_21d_Calm6_beta.fq.gz read1_86_21d_Calm6_beta.fq.gz | fastq fastq | 2610499200.0 | 8701664.0 | 86 21d Calm6 beta files | 0:150 1:150 | A:764524163;C:544491001;G:601533912;T:654396223;N:45553901 | 150 | 150 | 764524163 | 544491001 | 601533912 | 654396223 | 45553901 | SRX1583796 | SRS1295601 | SRA353254 | SRA | Bar-Ilan University | 2 | 0.00108 | 0.63356 | 0.00012 | 0.12284 | 0.99837 | 0.96193 | 0.21768 | 0.38237 | 150 | 150 | T | B | mate1 technical by mapping diff | illumina | hiseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | Israel | 2016-03-02 | Undetermined | Undetermined | Whole Organism | All anatomical structures | ||||||||||||||||||||
| 40398 | 40398 | SRR3166942 | SRX1583795 | SRS1295602 | SRP070056 | PRJNA309588 | Analysis of the T cell response in Zebrafish | PRJNA309588 | Other | Our understanding of T cell receptor TCR repertoire diversity and response tochallenge is still incomplete. For example TCR clones shared by different individuals withminimal alteration to germline gene sequences public clones are detectable in all vertebrates but their significance is unknown. We exploited the experimental advantages offered by thezebrafish to analyze the complete TCR repertoire and its response to self and foreign antigens.We found that cross reactive public TCRs dominate the T cell response endowing the TCRrepertoire with the ability to cope with diverse antigenic challenges. These features of vertebratepublic TCRs provide a mechanism for the rapid generation of protective T cell immunityallowing a short temporal window for the development of more specific private T cell responses. | TCRB library 21d Calm5 | breed:AB|chain:beta|index:43|sex:male|time point:21d|tissue:whole fish|age:1y|BioSampleModel:Model organism or animal | TCRb sequencing of zebrafish: whole zebrafish: Sample TCRB library 21d Calm5 | 85 21d Calm5 beta | 85 21d Calm5 beta | five prime RACE amplification of TCRb transcript | AMPLICON | TRANSCRIPTOMIC | RACE | PAIRED | ILLUMINA | Illumina HiSeq 2000 | <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>300</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>151</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> | SRP070056 | read1_85_21d_Calm5_beta.fq.gz read2_85_21d_Calm5_beta.fq.gz | fastq fastq | 2700056100.0 | 9000187.0 | 85 21d Calm5 beta files | 0:150 1:150 | A:755011216;C:593419169;G:623123170;T:688658373;N:39844172 | 150 | 150 | 755011216 | 593419169 | 623123170 | 688658373 | 39844172 | SRX1583795 | SRS1295602 | SRA353254 | SRA | Bar-Ilan University | 2 | 0.00244 | 0.68155 | 0.00026 | 0.01492 | 0.99766 | 0.96597 | 0.24863 | 0.31823 | 150 | 150 | T | B | mate1 technical by mapping diff | illumina | hiseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | Israel | 2017-02-12 | Undetermined | Undetermined | Whole Organism | All anatomical structures | ||||||||||||||||||||
| 40399 | 40399 | SRR3166941 | SRX1583794 | SRS1295603 | SRP070056 | PRJNA309588 | Analysis of the T cell response in Zebrafish | PRJNA309588 | Other | Our understanding of T cell receptor TCR repertoire diversity and response tochallenge is still incomplete. For example TCR clones shared by different individuals withminimal alteration to germline gene sequences public clones are detectable in all vertebrates but their significance is unknown. We exploited the experimental advantages offered by thezebrafish to analyze the complete TCR repertoire and its response to self and foreign antigens.We found that cross reactive public TCRs dominate the T cell response endowing the TCRrepertoire with the ability to cope with diverse antigenic challenges. These features of vertebratepublic TCRs provide a mechanism for the rapid generation of protective T cell immunityallowing a short temporal window for the development of more specific private T cell responses. | TCRB library 21d Calm1 | breed:AB|chain:beta|index:42|sex:male|time point:21d|tissue:whole fish|age:1y|BioSampleModel:Model organism or animal | TCRb sequencing of zebrafish: whole zebrafish: Sample TCRB library 21d Calm1 | 81 21d Calm1 beta | 81 21d Calm1 beta | five prime RACE amplification of TCRb transcript | AMPLICON | TRANSCRIPTOMIC | RACE | PAIRED | ILLUMINA | Illumina HiSeq 2000 | <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>300</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>151</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> | SRP070056 | read1_81_21d_Calm1_beta.fq.gz read2_81_21d_Calm1_beta.fq.gz | fastq fastq | 2296683900.0 | 7655613.0 | 81 21d Calm1 beta files | 0:150 1:150 | A:776751441;C:430253074;G:528909259;T:525320137;N:35449989 | 150 | 150 | 776751441 | 430253074 | 528909259 | 525320137 | 35449989 | SRX1583794 | SRS1295603 | SRA353254 | SRA | Bar-Ilan University | 2 | 2e-05 | 0.0464 | 0.0 | 0.00091 | 0.99997 | 0.99882 | 0.0 | 0.0134 | 150 | 150 | T | T | mates < 9% mapping rate | illumina | hiseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | Israel | 2016-03-02 | Undetermined | Undetermined | Whole Organism | All anatomical structures | ||||||||||||||||||||
| 40400 | 40400 | SRR3166940 | SRX1583793 | SRS1295604 | SRP070056 | PRJNA309588 | Analysis of the T cell response in Zebrafish | PRJNA309588 | Other | Our understanding of T cell receptor TCR repertoire diversity and response tochallenge is still incomplete. For example TCR clones shared by different individuals withminimal alteration to germline gene sequences public clones are detectable in all vertebrates but their significance is unknown. We exploited the experimental advantages offered by thezebrafish to analyze the complete TCR repertoire and its response to self and foreign antigens.We found that cross reactive public TCRs dominate the T cell response endowing the TCRrepertoire with the ability to cope with diverse antigenic challenges. These features of vertebratepublic TCRs provide a mechanism for the rapid generation of protective T cell immunityallowing a short temporal window for the development of more specific private T cell responses. | TCRB library 21d KLH6 | breed:AB|chain:beta|index:50|sex:male|time point:21d|tissue:whole fish|age:1y|BioSampleModel:Model organism or animal | TCRb sequencing of zebrafish: whole zebrafish: Sample TCRB library 21d KLH6 | 78 21d KLH6 beta | 78 21d KLH6 beta | five prime RACE amplification of TCRb transcript | AMPLICON | TRANSCRIPTOMIC | RACE | PAIRED | ILLUMINA | Illumina HiSeq 2000 | <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>300</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>151</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> | SRP070056 | read1_78_21d_KLH6_beta.fq.gz read2_78_21d_KLH6_beta.fq.gz | fastq fastq | 1500221100.0 | 5000737.0 | 78 21d KLH6 beta files | 0:150 1:150 | A:435807096;C:294707078;G:337922064;T:361615328;N:70169534 | 150 | 150 | 435807096 | 294707078 | 337922064 | 361615328 | 70169534 | SRX1583793 | SRS1295604 | SRA353254 | SRA | Bar-Ilan University | 2 | 5e-05 | 0.37212 | 3e-05 | 0.03128 | 0.99997 | 0.99358 | 0.0 | 0.11962 | 150 | 150 | T | B | mate1 technical by mapping diff | illumina | hiseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | Israel | 2017-02-12 | Undetermined | Undetermined | Whole Organism | All anatomical structures | ||||||||||||||||||||
| 40401 | 40401 | SRR3166939 | SRX1583792 | SRS1295605 | SRP070056 | PRJNA309588 | Analysis of the T cell response in Zebrafish | PRJNA309588 | Other | Our understanding of T cell receptor TCR repertoire diversity and response tochallenge is still incomplete. For example TCR clones shared by different individuals withminimal alteration to germline gene sequences public clones are detectable in all vertebrates but their significance is unknown. We exploited the experimental advantages offered by thezebrafish to analyze the complete TCR repertoire and its response to self and foreign antigens.We found that cross reactive public TCRs dominate the T cell response endowing the TCRrepertoire with the ability to cope with diverse antigenic challenges. These features of vertebratepublic TCRs provide a mechanism for the rapid generation of protective T cell immunityallowing a short temporal window for the development of more specific private T cell responses. | TCRB library 21d KLH5 | breed:AB|chain:beta|index:49|sex:male|time point:21d|tissue:whole fish|age:1y|BioSampleModel:Model organism or animal | TCRb sequencing of zebrafish: whole zebrafish: Sample TCRB library 21d KLH5 | 77 21d KLH5 beta | 77 21d KLH5 beta | five prime RACE amplification of TCRb transcript | AMPLICON | TRANSCRIPTOMIC | RACE | PAIRED | ILLUMINA | Illumina HiSeq 2000 | <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>300</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>151</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> | SRP070056 | read1_77_21d_KLH5_beta.fq.gz read2_77_21d_KLH5_beta.fq.gz | fastq fastq | 2106732600.0 | 7022442.0 | 77 21d KLH5 beta files | 0:150 1:150 | A:696373936;C:376059887;G:465978414;T:465485185;N:102835178 | 150 | 150 | 696373936 | 376059887 | 465978414 | 465485185 | 102835178 | SRX1583792 | SRS1295605 | SRA353254 | SRA | Bar-Ilan University | 2 | 0.0 | 0.04625 | 0.0 | 0.00381 | 1.0 | 0.99979 | 0.00123 | 150 | 150 | T | T | mates < 9% mapping rate | illumina | hiseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | Israel | 2017-02-12 | Undetermined | Undetermined | Whole Organism | All anatomical structures | |||||||||||||||||||||
| 40402 | 40402 | SRR3166938 | SRX1583791 | SRS1295606 | SRP070056 | PRJNA309588 | Analysis of the T cell response in Zebrafish | PRJNA309588 | Other | Our understanding of T cell receptor TCR repertoire diversity and response tochallenge is still incomplete. For example TCR clones shared by different individuals withminimal alteration to germline gene sequences public clones are detectable in all vertebrates but their significance is unknown. We exploited the experimental advantages offered by thezebrafish to analyze the complete TCR repertoire and its response to self and foreign antigens.We found that cross reactive public TCRs dominate the T cell response endowing the TCRrepertoire with the ability to cope with diverse antigenic challenges. These features of vertebratepublic TCRs provide a mechanism for the rapid generation of protective T cell immunityallowing a short temporal window for the development of more specific private T cell responses. | TCRB library 21d KLH1 | breed:AB|chain:beta|index:48|sex:male|time point:21d|tissue:whole fish|age:1y|BioSampleModel:Model organism or animal | TCRb sequencing of zebrafish: whole zebrafish: Sample TCRB library 21d KLH1 | 73 21d KLH1 beta | 73 21d KLH1 beta | five prime RACE amplification of TCRb transcript | AMPLICON | TRANSCRIPTOMIC | RACE | PAIRED | ILLUMINA | Illumina HiSeq 2000 | <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>300</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>151</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> | SRP070056 | read2_73_21d_KLH1_beta.fq.gz read1_73_21d_KLH1_beta.fq.gz | fastq fastq | 3554391900.0 | 11847973.0 | 73 21d KLH1 beta files | 0:150 1:150 | A:1312477977;C:598886813;G:787337227;T:686894043;N:168795840 | 150 | 150 | 1312477977 | 598886813 | 787337227 | 686894043 | 168795840 | SRX1583791 | SRS1295606 | SRA353254 | SRA | Bar-Ilan University | 2 | 6e-05 | 0.00136 | 5e-05 | 0.00019 | 1.0 | 0.99983 | 0.0303 | 150 | 150 | T | T | mates < 9% mapping rate | illumina | hiseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | Israel | 2017-02-12 | Undetermined | Undetermined | Whole Organism | All anatomical structures | |||||||||||||||||||||
| 40403 | 40403 | SRR3166937 | SRX1583790 | SRS1295607 | SRP070056 | PRJNA309588 | Analysis of the T cell response in Zebrafish | PRJNA309588 | Other | Our understanding of T cell receptor TCR repertoire diversity and response tochallenge is still incomplete. For example TCR clones shared by different individuals withminimal alteration to germline gene sequences public clones are detectable in all vertebrates but their significance is unknown. We exploited the experimental advantages offered by thezebrafish to analyze the complete TCR repertoire and its response to self and foreign antigens.We found that cross reactive public TCRs dominate the T cell response endowing the TCRrepertoire with the ability to cope with diverse antigenic challenges. These features of vertebratepublic TCRs provide a mechanism for the rapid generation of protective T cell immunityallowing a short temporal window for the development of more specific private T cell responses. | TCRB library 21d PHA6 | breed:AB|chain:beta|index:53|sex:male|time point:21d|tissue:whole fish|age:1y|BioSampleModel:Model organism or animal | TCRb sequencing of zebrafish: whole zebrafish: Sample TCRB library 21d PHA6 | 70 21d PHA6 beta | 70 21d PHA6 beta | five prime RACE amplification of TCRb transcript | AMPLICON | TRANSCRIPTOMIC | RACE | PAIRED | ILLUMINA | Illumina HiSeq 2000 | <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>300</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>151</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> | SRP070056 | read2_70_21d_PHA6_beta.fq.gz read1_70_21d_PHA6_beta.fq.gz | fastq fastq | 4238076900.0 | 14126923.0 | 70 21d PHA6 beta files | 0:150 1:150 | A:1519543327;C:710498920;G:932091678;T:844220454;N:231722521 | 150 | 150 | 1519543327 | 710498920 | 932091678 | 844220454 | 231722521 | SRX1583790 | SRS1295607 | SRA353254 | SRA | Bar-Ilan University | 2 | 0.00023 | 0.00772 | 7e-05 | 0.00068 | 0.99991 | 0.99851 | 0.25 | 0.08615 | 150 | 150 | T | T | mates < 9% mapping rate | illumina | hiseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | Israel | 2017-02-12 | Undetermined | Undetermined | Whole Organism | All anatomical structures | ||||||||||||||||||||
| 40404 | 40404 | SRR3166936 | SRX1583789 | SRS1295608 | SRP070056 | PRJNA309588 | Analysis of the T cell response in Zebrafish | PRJNA309588 | Other | Our understanding of T cell receptor TCR repertoire diversity and response tochallenge is still incomplete. For example TCR clones shared by different individuals withminimal alteration to germline gene sequences public clones are detectable in all vertebrates but their significance is unknown. We exploited the experimental advantages offered by thezebrafish to analyze the complete TCR repertoire and its response to self and foreign antigens.We found that cross reactive public TCRs dominate the T cell response endowing the TCRrepertoire with the ability to cope with diverse antigenic challenges. These features of vertebratepublic TCRs provide a mechanism for the rapid generation of protective T cell immunityallowing a short temporal window for the development of more specific private T cell responses. | TCRB library 21d PHA4 | breed:AB|chain:beta|index:52|sex:male|time point:21d|tissue:whole fish|age:1y|BioSampleModel:Model organism or animal | TCRb sequencing of zebrafish: whole zebrafish: Sample TCRB library 21d PHA4 | 68 21d PHA4 beta | 68 21d PHA4 beta | five prime RACE amplification of TCRb transcript | AMPLICON | TRANSCRIPTOMIC | RACE | PAIRED | ILLUMINA | Illumina HiSeq 2000 | <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>300</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>151</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> | SRP070056 | read1_68_21d_PHA4_beta.fq.gz read2_68_21d_PHA4_beta.fq.gz | fastq fastq | 2030511600.0 | 6768372.0 | 68 21d PHA4 beta files | 0:150 1:150 | A:659873688;C:307551211;G:388669539;T:566328083;N:108089079 | 150 | 150 | 659873688 | 307551211 | 388669539 | 566328083 | 108089079 | SRX1583789 | SRS1295608 | SRA353254 | SRA | Bar-Ilan University | 2 | 0.00029 | 0.24246 | 3e-05 | 0.05929 | 0.99971 | 0.99214 | 0.4 | 0.16492 | 150 | 150 | T | B | mate1 technical by mapping diff | illumina | hiseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | Israel | 2017-02-12 | Undetermined | Undetermined | Whole Organism | All anatomical structures | ||||||||||||||||||||
| 40405 | 40405 | SRR3166935 | SRX1583788 | SRS1295609 | SRP070056 | PRJNA309588 | Analysis of the T cell response in Zebrafish | PRJNA309588 | Other | Our understanding of T cell receptor TCR repertoire diversity and response tochallenge is still incomplete. For example TCR clones shared by different individuals withminimal alteration to germline gene sequences public clones are detectable in all vertebrates but their significance is unknown. We exploited the experimental advantages offered by thezebrafish to analyze the complete TCR repertoire and its response to self and foreign antigens.We found that cross reactive public TCRs dominate the T cell response endowing the TCRrepertoire with the ability to cope with diverse antigenic challenges. These features of vertebratepublic TCRs provide a mechanism for the rapid generation of protective T cell immunityallowing a short temporal window for the development of more specific private T cell responses. | TCRB library 21d PHA1 | breed:AB|chain:beta|index:51|sex:male|time point:21d|tissue:whole fish|age:1y|BioSampleModel:Model organism or animal | TCRb sequencing of zebrafish: whole zebrafish: Sample TCRB library 21d PHA1 | 65 21d PHA1 beta | 65 21d PHA1 beta | five prime RACE amplification of TCRb transcript | AMPLICON | TRANSCRIPTOMIC | RACE | PAIRED | ILLUMINA | Illumina HiSeq 2000 | <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>300</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>151</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> | SRP070056 | read1_65_21d_PHA1_beta.fq.gz read2_65_21d_PHA1_beta.fq.gz | fastq fastq | 930240600.0 | 3100802.0 | 65 21d PHA1 beta files | 0:150 1:150 | A:259486434;C:184043790;G:197463162;T:250422190;N:38825024 | 150 | 150 | 259486434 | 184043790 | 197463162 | 250422190 | 38825024 | SRX1583788 | SRS1295609 | SRA353254 | SRA | Bar-Ilan University | 2 | 0.00012 | 0.14868 | 0.0 | 0.02051 | 0.99963 | 0.99111 | 0.36842 | 0.258 | 150 | 150 | T | B | mate1 technical by mapping diff | illumina | hiseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | Israel | 2017-02-12 | Undetermined | Undetermined | Whole Organism | All anatomical structures | ||||||||||||||||||||
| 40406 | 40406 | SRR3166934 | SRX1583787 | SRS1295610 | SRP070056 | PRJNA309588 | Analysis of the T cell response in Zebrafish | PRJNA309588 | Other | Our understanding of T cell receptor TCR repertoire diversity and response tochallenge is still incomplete. For example TCR clones shared by different individuals withminimal alteration to germline gene sequences public clones are detectable in all vertebrates but their significance is unknown. We exploited the experimental advantages offered by thezebrafish to analyze the complete TCR repertoire and its response to self and foreign antigens.We found that cross reactive public TCRs dominate the T cell response endowing the TCRrepertoire with the ability to cope with diverse antigenic challenges. These features of vertebratepublic TCRs provide a mechanism for the rapid generation of protective T cell immunityallowing a short temporal window for the development of more specific private T cell responses. | TCRB library 21d IFA 8 | breed:AB|chain:beta|index:47|sex:male|time point:21d|tissue:whole fish|age:1y|BioSampleModel:Model organism or animal | TCRb sequencing of zebrafish: whole zebrafish: Sample TCRB library 21d IFA 8 | 64 21d IFA 8 beta | 64 21d IFA 8 beta | five prime RACE amplification of TCRb transcript | AMPLICON | TRANSCRIPTOMIC | RACE | PAIRED | ILLUMINA | Illumina HiSeq 2000 | <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>300</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>151</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> | SRP070056 | read1_64_21d_IFA_8_beta.fq.gz read2_64_21d_IFA_8_beta.fq.gz | fastq fastq | 1461868800.0 | 4872896.0 | 64 21d IFA 8 beta files | 0:150 1:150 | A:462418747;C:247121586;G:284662907;T:394116911;N:73548649 | 150 | 150 | 462418747 | 247121586 | 284662907 | 394116911 | 73548649 | SRX1583787 | SRS1295610 | SRA353254 | SRA | Bar-Ilan University | 2 | 0.0001 | 0.17532 | 0.0 | 0.04988 | 0.99983 | 0.99843 | 0.25 | 0.01908 | 150 | 150 | T | B | mate1 technical by mapping diff | illumina | hiseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | Israel | 2017-02-12 | Undetermined | Undetermined | Whole Organism | All anatomical structures | ||||||||||||||||||||
| 40407 | 40407 | SRR3166933 | SRX1583786 | SRS1295611 | SRP070056 | PRJNA309588 | Analysis of the T cell response in Zebrafish | PRJNA309588 | Other | Our understanding of T cell receptor TCR repertoire diversity and response tochallenge is still incomplete. For example TCR clones shared by different individuals withminimal alteration to germline gene sequences public clones are detectable in all vertebrates but their significance is unknown. We exploited the experimental advantages offered by thezebrafish to analyze the complete TCR repertoire and its response to self and foreign antigens.We found that cross reactive public TCRs dominate the T cell response endowing the TCRrepertoire with the ability to cope with diverse antigenic challenges. These features of vertebratepublic TCRs provide a mechanism for the rapid generation of protective T cell immunityallowing a short temporal window for the development of more specific private T cell responses. | TCRB library 21d IFA 4 | breed:AB|chain:beta|index:46|sex:male|time point:21d|tissue:whole fish|age:1y|BioSampleModel:Model organism or animal | TCRb sequencing of zebrafish: whole zebrafish: Sample TCRB library 21d IFA 4 | 60 21d IFA 4 beta | 60 21d IFA 4 beta | five prime RACE amplification of TCRb transcript | AMPLICON | TRANSCRIPTOMIC | RACE | PAIRED | ILLUMINA | Illumina HiSeq 2000 | <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>300</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>151</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> | SRP070056 | read2_60_21d_IFA_4_beta.fq.gz read1_60_21d_IFA_4_beta.fq.gz | fastq fastq | 4128399900.0 | 13761333.0 | 60 21d IFA 4 beta files | 0:150 1:150 | A:1332478795;C:800389451;G:937176538;T:950735272;N:107619844 | 150 | 150 | 1332478795 | 800389451 | 937176538 | 950735272 | 107619844 | SRX1583786 | SRS1295611 | SRA353254 | SRA | Bar-Ilan University | 2 | 0.00043 | 0.19668 | 5e-05 | 0.00597 | 0.99953 | 0.9782 | 0.2647 | 0.35656 | 150 | 150 | T | B | mate1 technical by mapping diff | illumina | hiseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | Israel | 2017-02-12 | Undetermined | Undetermined | Whole Organism | All anatomical structures | ||||||||||||||||||||
| 40408 | 40408 | SRR3166932 | SRX1583785 | SRS1295612 | SRP070056 | PRJNA309588 | Analysis of the T cell response in Zebrafish | PRJNA309588 | Other | Our understanding of T cell receptor TCR repertoire diversity and response tochallenge is still incomplete. For example TCR clones shared by different individuals withminimal alteration to germline gene sequences public clones are detectable in all vertebrates but their significance is unknown. We exploited the experimental advantages offered by thezebrafish to analyze the complete TCR repertoire and its response to self and foreign antigens.We found that cross reactive public TCRs dominate the T cell response endowing the TCRrepertoire with the ability to cope with diverse antigenic challenges. These features of vertebratepublic TCRs provide a mechanism for the rapid generation of protective T cell immunityallowing a short temporal window for the development of more specific private T cell responses. | TCRB library 21d IFA 2 | breed:AB|chain:beta|index:45|sex:male|time point:21d|tissue:whole fish|age:1y|BioSampleModel:Model organism or animal | TCRb sequencing of zebrafish: whole zebrafish: Sample TCRB library 21d IFA 2 | 58 21d IFA 2 beta | 58 21d IFA 2 beta | five prime RACE amplification of TCRb transcript | AMPLICON | TRANSCRIPTOMIC | RACE | PAIRED | ILLUMINA | Illumina HiSeq 2000 | <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>300</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>151</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> | SRP070056 | read2_58_21d_IFA_2_beta.fq.gz read1_58_21d_IFA_2_beta.fq.gz | fastq fastq | 5035078800.0 | 16783596.0 | 58 21d IFA 2 beta files | 0:150 1:150 | A:1714919930;C:839542402;G:1121993893;T:1220614755;N:138007820 | 150 | 150 | 1714919930 | 839542402 | 1121993893 | 1220614755 | 138007820 | SRX1583785 | SRS1295612 | SRA353254 | SRA | Bar-Ilan University | 2 | 0.0 | 0.17551 | 0.0 | 0.00318 | 1.0 | 0.99833 | 0.10802 | 150 | 150 | T | B | mate1 technical by mapping diff | illumina | hiseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | Israel | 2016-03-02 | Undetermined | Undetermined | Whole Organism | All anatomical structures | |||||||||||||||||||||
| 40409 | 40409 | SRR3166931 | SRX1583784 | SRS1295613 | SRP070056 | PRJNA309588 | Analysis of the T cell response in Zebrafish | PRJNA309588 | Other | Our understanding of T cell receptor TCR repertoire diversity and response tochallenge is still incomplete. For example TCR clones shared by different individuals withminimal alteration to germline gene sequences public clones are detectable in all vertebrates but their significance is unknown. We exploited the experimental advantages offered by thezebrafish to analyze the complete TCR repertoire and its response to self and foreign antigens.We found that cross reactive public TCRs dominate the T cell response endowing the TCRrepertoire with the ability to cope with diverse antigenic challenges. These features of vertebratepublic TCRs provide a mechanism for the rapid generation of protective T cell immunityallowing a short temporal window for the development of more specific private T cell responses. | TCRB library 14d Calm6 | breed:AB|chain:beta|index:37|sex:male|time point:14d|tissue:whole fish|age:1y|BioSampleModel:Model organism or animal | TCRb sequencing of zebrafish: whole zebrafish: Sample TCRB library 14d Calm6 | 55 14d Calm6 beta | 55 14d Calm6 beta | five prime RACE amplification of TCRb transcript | AMPLICON | TRANSCRIPTOMIC | RACE | PAIRED | ILLUMINA | Illumina HiSeq 2000 | <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>300</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>151</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> | SRP070056 | read1_55_14d_Calm6_beta.fq.gz read2_55_14d_Calm6_beta.fq.gz | fastq fastq | 1028012700.0 | 3426709.0 | 55 14d Calm6 beta files | 0:150 1:150 | A:295958360;C:207583542;G:216651025;T:284035600;N:23784173 | 150 | 150 | 295958360 | 207583542 | 216651025 | 284035600 | 23784173 | SRX1583784 | SRS1295613 | SRA353254 | SRA | Bar-Ilan University | 2 | 5e-05 | 0.16498 | 0.0 | 0.01748 | 0.99989 | 0.99253 | 0.33333 | 0.12385 | 150 | 150 | T | B | mate1 technical by mapping diff | illumina | hiseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | Israel | 2016-03-02 | Undetermined | Undetermined | Whole Organism | All anatomical structures | ||||||||||||||||||||
| 40410 | 40410 | SRR3166930 | SRX1583783 | SRS1295614 | SRP070056 | PRJNA309588 | Analysis of the T cell response in Zebrafish | PRJNA309588 | Other | Our understanding of T cell receptor TCR repertoire diversity and response tochallenge is still incomplete. For example TCR clones shared by different individuals withminimal alteration to germline gene sequences public clones are detectable in all vertebrates but their significance is unknown. We exploited the experimental advantages offered by thezebrafish to analyze the complete TCR repertoire and its response to self and foreign antigens.We found that cross reactive public TCRs dominate the T cell response endowing the TCRrepertoire with the ability to cope with diverse antigenic challenges. These features of vertebratepublic TCRs provide a mechanism for the rapid generation of protective T cell immunityallowing a short temporal window for the development of more specific private T cell responses. | TCRB library Naive6 | breed:AB|chain:beta|index:31|sex:male|tissue:whole fish|age:1y|BioSampleModel:Model organism or animal | TCRb sequencing of zebrafish: whole zebrafish: Sample TCRB library Naive6 | 126 Naive6 beta | 126 Naive6 beta | five prime RACE amplification of TCRb transcript | AMPLICON | TRANSCRIPTOMIC | RACE | PAIRED | ILLUMINA | Illumina HiSeq 2000 | <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>300</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>151</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> | SRP070056 | read2_126_Naive6_beta.fq.gz read1_126_Naive6_beta.fq.gz | fastq fastq | 1984909800.0 | 6616366.0 | 126 Naive6 beta files | 0:150 1:150 | A:574002364;C:411972224;G:452963561;T:493883048;N:52088603 | 150 | 150 | 574002364 | 411972224 | 452963561 | 493883048 | 52088603 | SRX1583783 | SRS1295614 | SRA353254 | SRA | Bar-Ilan University | 2 | 0.0007 | 0.73767 | 5e-05 | 0.01562 | 0.99928 | 0.9754 | 0.16 | 0.44061 | 150 | 150 | T | B | mate1 technical by mapping diff | illumina | hiseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | Israel | 2017-02-12 | Undetermined | Undetermined | Whole Organism | All anatomical structures | ||||||||||||||||||||
| 40411 | 40411 | SRR3166929 | SRX1583782 | SRS1295615 | SRP070056 | PRJNA309588 | Analysis of the T cell response in Zebrafish | PRJNA309588 | Other | Our understanding of T cell receptor TCR repertoire diversity and response tochallenge is still incomplete. For example TCR clones shared by different individuals withminimal alteration to germline gene sequences public clones are detectable in all vertebrates but their significance is unknown. We exploited the experimental advantages offered by thezebrafish to analyze the complete TCR repertoire and its response to self and foreign antigens.We found that cross reactive public TCRs dominate the T cell response endowing the TCRrepertoire with the ability to cope with diverse antigenic challenges. These features of vertebratepublic TCRs provide a mechanism for the rapid generation of protective T cell immunityallowing a short temporal window for the development of more specific private T cell responses. | TCRB library 14d IFA1 | breed:AB|chain:beta|index:38|sex:male|time point:14d|tissue:whole fish|age:1y|BioSampleModel:Model organism or animal | TCRb sequencing of zebrafish: whole zebrafish: Sample TCRB library 14d IFA1 | 36 14d IFA1 beta | 36 14d IFA1 beta | five prime RACE amplification of TCRb transcript | AMPLICON | TRANSCRIPTOMIC | RACE | PAIRED | ILLUMINA | Illumina HiSeq 2000 | <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>300</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>151</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> | SRP070056 | read2_36_14d_IFA1_beta.fq.gz read1_36_14d_IFA1_beta.fq.gz | fastq fastq | 2641709400.0 | 8805698.0 | 36 14d IFA1 beta files | 0:150 1:150 | A:828430279;C:516389015;G:603137557;T:652458512;N:41294037 | 150 | 150 | 828430279 | 516389015 | 603137557 | 652458512 | 41294037 | SRX1583782 | SRS1295615 | SRA353254 | SRA | Bar-Ilan University | 2 | 6e-05 | 0.17777 | 1e-05 | 0.00441 | 0.99991 | 0.99243 | 0.5 | 0.07212 | 150 | 150 | T | B | mate1 technical by mapping diff | illumina | hiseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | Israel | 2017-02-12 | Undetermined | Undetermined | Whole Organism | All anatomical structures | ||||||||||||||||||||
| 40412 | 40412 | SRR3166928 | SRX1583781 | SRS1295616 | SRP070056 | PRJNA309588 | Analysis of the T cell response in Zebrafish | PRJNA309588 | Other | Our understanding of T cell receptor TCR repertoire diversity and response tochallenge is still incomplete. For example TCR clones shared by different individuals withminimal alteration to germline gene sequences public clones are detectable in all vertebrates but their significance is unknown. We exploited the experimental advantages offered by thezebrafish to analyze the complete TCR repertoire and its response to self and foreign antigens.We found that cross reactive public TCRs dominate the T cell response endowing the TCRrepertoire with the ability to cope with diverse antigenic challenges. These features of vertebratepublic TCRs provide a mechanism for the rapid generation of protective T cell immunityallowing a short temporal window for the development of more specific private T cell responses. | TCRB library 14d PHA7 | breed:AB|chain:beta|index:41|sex:male|time point:14d|tissue:whole fish|age:1y|BioSampleModel:Model organism or animal | TCRb sequencing of zebrafish: whole zebrafish: Sample TCRB library 14d PHA7 | 35 14d PHA7 beta | 35 14d PHA7 beta | five prime RACE amplification of TCRb transcript | AMPLICON | TRANSCRIPTOMIC | RACE | PAIRED | ILLUMINA | Illumina HiSeq 2000 | <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>300</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>151</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> | SRP070056 | read2_35_14d_PHA7_beta.fq.gz read1_35_14d_PHA7_beta.fq.gz | fastq fastq | 2481744900.0 | 8272483.0 | 35 14d PHA7 beta files | 0:150 1:150 | A:684148801;C:534638425;G:555073374;T:671216692;N:36667608 | 150 | 150 | 684148801 | 534638425 | 555073374 | 671216692 | 36667608 | SRX1583781 | SRS1295616 | SRA353254 | SRA | Bar-Ilan University | 2 | 0.00054 | 0.3447 | 8e-05 | 0.03511 | 0.9991 | 0.97049 | 0.29411 | 0.37357 | 150 | 150 | T | B | mate1 technical by mapping diff | illumina | hiseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | Israel | 2017-02-12 | Undetermined | Undetermined | Whole Organism | All anatomical structures | ||||||||||||||||||||
| 40413 | 40413 | SRR3166927 | SRX1583780 | SRS1295617 | SRP070056 | PRJNA309588 | Analysis of the T cell response in Zebrafish | PRJNA309588 | Other | Our understanding of T cell receptor TCR repertoire diversity and response tochallenge is still incomplete. For example TCR clones shared by different individuals withminimal alteration to germline gene sequences public clones are detectable in all vertebrates but their significance is unknown. We exploited the experimental advantages offered by thezebrafish to analyze the complete TCR repertoire and its response to self and foreign antigens.We found that cross reactive public TCRs dominate the T cell response endowing the TCRrepertoire with the ability to cope with diverse antigenic challenges. These features of vertebratepublic TCRs provide a mechanism for the rapid generation of protective T cell immunityallowing a short temporal window for the development of more specific private T cell responses. | TCRB library 14d PHA6 | breed:AB|chain:beta|index:40|sex:male|time point:14d|tissue:whole fish|age:1y|BioSampleModel:Model organism or animal | TCRb sequencing of zebrafish: whole zebrafish: Sample TCRB library 14d PHA6 | 34 14d PHA6 beta | 34 14d PHA6 beta | five prime RACE amplification of TCRb transcript | AMPLICON | TRANSCRIPTOMIC | RACE | PAIRED | ILLUMINA | Illumina HiSeq 2000 | <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>300</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>151</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> | SRP070056 | read1_34_14d_PHA6_beta.fq.gz read2_34_14d_PHA6_beta.fq.gz | fastq fastq | 2149899000.0 | 7166330.0 | 34 14d PHA6 beta files | 0:150 1:150 | A:603640552;C:452244383;G:474826068;T:587177894;N:32010103 | 150 | 150 | 603640552 | 452244383 | 474826068 | 587177894 | 32010103 | SRX1583780 | SRS1295617 | SRA353254 | SRA | Bar-Ilan University | 2 | 4e-05 | 0.16994 | 0.0 | 0.14727 | 0.99991 | 0.99342 | 0.0 | 0.31047 | 150 | 150 | T | B | mate1 technical by mapping diff | illumina | hiseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | Israel | 2016-03-02 | Undetermined | Undetermined | Whole Organism | All anatomical structures | ||||||||||||||||||||
| 40414 | 40414 | SRR3166926 | SRX1583779 | SRS1295618 | SRP070056 | PRJNA309588 | Analysis of the T cell response in Zebrafish | PRJNA309588 | Other | Our understanding of T cell receptor TCR repertoire diversity and response tochallenge is still incomplete. For example TCR clones shared by different individuals withminimal alteration to germline gene sequences public clones are detectable in all vertebrates but their significance is unknown. We exploited the experimental advantages offered by thezebrafish to analyze the complete TCR repertoire and its response to self and foreign antigens.We found that cross reactive public TCRs dominate the T cell response endowing the TCRrepertoire with the ability to cope with diverse antigenic challenges. These features of vertebratepublic TCRs provide a mechanism for the rapid generation of protective T cell immunityallowing a short temporal window for the development of more specific private T cell responses. | TCRB library 14d PHA5 | breed:AB|chain:beta|index:39|sex:male|time point:14d|tissue:whole fish|age:1y|BioSampleModel:Model organism or animal | TCRb sequencing of zebrafish: whole zebrafish: Sample TCRB library 14d PHA5 | 33 14d PHA5 beta | 33 14d PHA5 beta | five prime RACE amplification of TCRb transcript | AMPLICON | TRANSCRIPTOMIC | RACE | PAIRED | ILLUMINA | Illumina HiSeq 2000 | <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>300</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>151</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> | SRP070056 | read1_33_14d_PHA5_beta.fq.gz read2_33_14d_PHA5_beta.fq.gz | fastq fastq | 4781329500.0 | 15937765.0 | 33 14d PHA5 beta files | 0:150 1:150 | A:1564269921;C:790001191;G:1015752339;T:1331170138;N:80135911 | 150 | 150 | 1564269921 | 790001191 | 1015752339 | 1331170138 | 80135911 | SRX1583779 | SRS1295618 | SRA353254 | SRA | Bar-Ilan University | 2 | 0.00034 | 0.20127 | 0.00018 | 0.00887 | 0.99989 | 0.99567 | 0.05263 | 0.08667 | 150 | 150 | T | B | mate1 technical by mapping diff | illumina | hiseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | Israel | 2016-03-02 | Undetermined | Undetermined | Whole Organism | All anatomical structures |
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CREATE TABLE run_metadata("run.accession" VARCHAR, "experiment.accession" VARCHAR, "sample.accession" VARCHAR, "study.accession" VARCHAR, bioproject VARCHAR, "study.title" VARCHAR, "study.alias" VARCHAR, "study.type" VARCHAR, "study.abstract" VARCHAR, "study.attributes" VARCHAR, "study.PMIDs" VARCHAR, "sample.description" VARCHAR, "sample.title" VARCHAR, "sample.alias" VARCHAR, "sample.centername" VARCHAR, "sample.attributes" VARCHAR, "GEOsample.title" VARCHAR, "GEOsample.dataprocessing" VARCHAR, "GEOsample.source" VARCHAR, "GEOsample.treatmentprotocol" VARCHAR, "GEOsample.extractprotocol" VARCHAR, "GEOsample.growthprotocol" VARCHAR, "GEOsample.characteristics" VARCHAR, "GEOsample.accession" VARCHAR, "experiment.title" VARCHAR, "experiment.alias" VARCHAR, "experiment.library_name" VARCHAR, "experiment.design_description" VARCHAR, "experiment.library_construction_protocol" VARCHAR, "experiment.attributes" VARCHAR, "experiment.library_strategy" VARCHAR, "experiment.library_source" VARCHAR, "experiment.library_selection" VARCHAR, "experiment.library_layout" VARCHAR, "experiment.platform" VARCHAR, "experiment.instrument_model" VARCHAR, "experiment.spot_descriptor" VARCHAR, "experiment.study_ref" VARCHAR, "run.title" VARCHAR, "run.attributes" VARCHAR, "run.filename" VARCHAR, "run.semantic_name" VARCHAR, "run.total_bases" DOUBLE, "run.total_spots" DOUBLE, "run.alias" VARCHAR, "run.read_lengths" VARCHAR, "run.base_counts" VARCHAR, "run.r1_length" BIGINT, "run.r2_length" BIGINT, "run.r3_length" BIGINT, "run.r4_length" BIGINT, "run.Acount" BIGINT, "run.Ccount" BIGINT, "run.Gcount" BIGINT, "run.Tcount" BIGINT, "run.Ncount" BIGINT, "run.experiment" VARCHAR, "run.pool_member" VARCHAR, "submission.accession" VARCHAR, "submission.srasource" VARCHAR, "submission.bioprojectsource" VARCHAR, "seqdetective.n_mates" BIGINT, "seqdetective.mapping_rate.mate1" DOUBLE, "seqdetective.mapping_rate.mate2" DOUBLE, "seqdetective.nofeature_rate.mate1" DOUBLE, "seqdetective.nofeature_rate.mate2" DOUBLE, "seqdetective.sparsity.mate1" DOUBLE, "seqdetective.sparsity.mate2" DOUBLE, "seqdetective.pos_strand_rate.mate1" DOUBLE, "seqdetective.pos_strand_rate.mate2" DOUBLE, "seqdetective.readlen.mate1" BIGINT, "seqdetective.readlen.mate2" BIGINT, "seqdetective.judgement.mate1" VARCHAR, "seqdetective.judgement.mate2" VARCHAR, "seqdetective.judgement.reason" VARCHAR, platform_family VARCHAR, instrument_generation VARCHAR, read_bias VARCHAR, selection_class VARCHAR, prep_kit VARCHAR, sc_or_bulk VARCHAR, tech_class VARCHAR, technology VARCHAR, tech_variant VARCHAR, "submission.bioprojectsource.country" VARCHAR, earliest_date DATE, devstage_curation VARCHAR, devstage_curation_coarse VARCHAR, tissue_curation VARCHAR, tissue_curation_coarse VARCHAR);;
CREATE INDEX idx_run_bioproject ON run_metadata(bioproject);;
CREATE INDEX idx_run_run_accession ON run_metadata("run.accession");;