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163 rows where experiment.library_selection = "PolyA", experiment.platform = "ILLUMINA" and tissue_curation_coarse = "Nervous System"

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technology 3

tissue_curation 2

experiment.library_source 2

experiment.library_layout 2

devstage_curation_coarse 2

devstage_curation 2

tissue_curation_coarse 1

  • Nervous System · 163 ✖

experiment.platform 1

  • ILLUMINA · 163 ✖

experiment.library_strategy 1

experiment.library_selection 1

  • PolyA · 163 ✖
Link rowid ▼ run.accession experiment.accession sample.accession study.accession bioproject study.title study.alias study.type study.abstract study.attributes study.PMIDs sample.description sample.title sample.alias sample.centername sample.attributes GEOsample.title GEOsample.dataprocessing GEOsample.source GEOsample.treatmentprotocol GEOsample.extractprotocol GEOsample.growthprotocol GEOsample.characteristics GEOsample.accession experiment.title experiment.alias experiment.library_name experiment.design_description experiment.library_construction_protocol experiment.attributes experiment.library_strategy experiment.library_source experiment.library_selection experiment.library_layout experiment.platform experiment.instrument_model experiment.spot_descriptor experiment.study_ref run.title run.attributes run.filename run.semantic_name run.total_bases run.total_spots run.alias run.read_lengths run.base_counts run.r1_length run.r2_length run.r3_length run.r4_length run.Acount run.Ccount run.Gcount run.Tcount run.Ncount run.experiment run.pool_member submission.accession submission.srasource submission.bioprojectsource seqdetective.n_mates seqdetective.mapping_rate.mate1 seqdetective.mapping_rate.mate2 seqdetective.nofeature_rate.mate1 seqdetective.nofeature_rate.mate2 seqdetective.sparsity.mate1 seqdetective.sparsity.mate2 seqdetective.pos_strand_rate.mate1 seqdetective.pos_strand_rate.mate2 seqdetective.readlen.mate1 seqdetective.readlen.mate2 seqdetective.judgement.mate1 seqdetective.judgement.mate2 seqdetective.judgement.reason platform_family instrument_generation read_bias selection_class prep_kit sc_or_bulk tech_class technology tech_variant submission.bioprojectsource.country earliest_date devstage_curation devstage_curation_coarse tissue_curation tissue_curation_coarse
101 101 DRR189403 DRX179868 DRS200418 DRP003977 PRJDB4470 Gene expression analysis of the zebrafish brain DRP003977 Other Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.       Telencephalon of adult zebrafrish 30 min post test session of 2 weeks memory test in non trace 2 Way Active Avoidance coditioning 3 SAMD00182246   sample name:CSUS Tel 30 2w memory 3|genotype:wild type|tissue:brain                 Illumina HiSeq 3000 sequencing of SAMD00182246 DRX179868 CSUS Tel 30 2w memory 3 1 SureSelect Strand Specific RNA Library Prep Kit   RNA-Seq TRANSCRIPTOMIC PolyA SINGLE ILLUMINA Illumina HiSeq 3000 <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>36</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> DRP003977 Illumina HiSeq 3000 sequencing of SAMD00182246       2791119960.0 77531110.0 DRR189403 0:36 A:685050951;C:641519684;G:664655385;T:799677669;N:216271 36       685050951 641519684 664655385 799677669 216271 DRX179868 DRS200418 DRA008865 NIG|National Institute of Genetics (Japan) National Institute of Genetics (Japan) 1 0.89653   0.1773   0.70725   0.49873   36   B   usable mapping rate illumina hiseq_era unknown poly_a unknown bulk unknown unknown   Japan 2021-08-08 Larval Larval Brain Nervous System
102 102 DRR189402 DRX179867 DRS200417 DRP003977 PRJDB4470 Gene expression analysis of the zebrafish brain DRP003977 Other Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.       Telencephalon of adult zebrafrish 30 min post test session of 2 weeks memory test in non trace 2 Way Active Avoidance coditioning 2 SAMD00182245   sample name:CSUS Tel 30 2w memory 2|genotype:wild type|tissue:brain                 Illumina HiSeq 3000 sequencing of SAMD00182245 DRX179867 CSUS Tel 30 2w memory 2 1 SureSelect Strand Specific RNA Library Prep Kit   RNA-Seq TRANSCRIPTOMIC PolyA SINGLE ILLUMINA Illumina HiSeq 3000 <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>36</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> DRP003977 Illumina HiSeq 3000 sequencing of SAMD00182245       1505449224.0 41818034.0 DRR189402 0:36 A:369895057;C:346914787;G:358624651;T:429897489;N:117240 36       369895057 346914787 358624651 429897489 117240 DRX179867 DRS200417 DRA008865 NIG|National Institute of Genetics (Japan) National Institute of Genetics (Japan) 1 0.89514   0.17677   0.7025   0.49571   36   B   usable mapping rate illumina hiseq_era unknown poly_a unknown bulk unknown unknown   Japan 2021-08-08 Larval Larval Brain Nervous System
103 103 DRR189401 DRX179866 DRS200416 DRP003977 PRJDB4470 Gene expression analysis of the zebrafish brain DRP003977 Other Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.       Telencephalon of adult zebrafrish 30 min post test session of 2 weeks memory test in non trace 2 Way Active Avoidance coditioning 1 SAMD00182244   sample name:CSUS Tel 30 2w memory 1|genotype:wild type|tissue:brain                 Illumina HiSeq 3000 sequencing of SAMD00182244 DRX179866 CSUS Tel 30 2w memory 1 1 SureSelect Strand Specific RNA Library Prep Kit   RNA-Seq TRANSCRIPTOMIC PolyA SINGLE ILLUMINA Illumina HiSeq 3000 <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>36</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> DRP003977 Illumina HiSeq 3000 sequencing of SAMD00182244       2088507024.0 58014084.0 DRR189401 0:36 A:516255405;C:478036869;G:496415722;T:597637091;N:161937 36       516255405 478036869 496415722 597637091 161937 DRX179866 DRS200416 DRA008865 NIG|National Institute of Genetics (Japan) National Institute of Genetics (Japan) 1 0.89533   0.18553   0.70981   0.49554   36   B   usable mapping rate illumina hiseq_era unknown poly_a unknown bulk unknown unknown   Japan 2021-08-08 Larval Larval Brain Nervous System
104 104 DRR189400 DRX179865 DRS200401 DRP003977 PRJDB4470 Gene expression analysis of the zebrafish brain DRP003977 Other Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.       Telencephalon of adult zebrafrish 30 min post test session of 1 day memory test in non trace 2 Way Active Avoidance coditioning 3 SAMD00182243   sample name:CSUS Tel 30 1d memory 3|genotype:wild type|tissue:brain                 Illumina HiSeq 3000 sequencing of SAMD00182243 DRX179865 CSUS Tel 30 1d memory 3 1 SureSelect Strand Specific RNA Library Prep Kit   RNA-Seq TRANSCRIPTOMIC PolyA SINGLE ILLUMINA Illumina HiSeq 3000 <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>36</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> DRP003977 Illumina HiSeq 3000 sequencing of SAMD00182243       1340808120.0 37244670.0 DRR189400 0:36 A:330513975;C:306935584;G:320089399;T:383165274;N:103888 36       330513975 306935584 320089399 383165274 103888 DRX179865 DRS200401 DRA008864 NIG|National Institute of Genetics (Japan) National Institute of Genetics (Japan) 1 0.89272   0.19494   0.70335   0.49456   36   B   usable mapping rate illumina hiseq_era unknown poly_a unknown bulk unknown unknown   Japan 2021-08-08 Adult Adult Brain Nervous System
105 105 DRR189399 DRX179864 DRS200400 DRP003977 PRJDB4470 Gene expression analysis of the zebrafish brain DRP003977 Other Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.       Telencephalon of adult zebrafrish 30 min post test session of 1 day memory test in non trace 2 Way Active Avoidance coditioning 2 SAMD00182242   sample name:CSUS Tel 30 1d memory 2|genotype:wild type|tissue:brain                 Illumina HiSeq 3000 sequencing of SAMD00182242 DRX179864 CSUS Tel 30 1d memory 2 1 SureSelect Strand Specific RNA Library Prep Kit   RNA-Seq TRANSCRIPTOMIC PolyA SINGLE ILLUMINA Illumina HiSeq 3000 <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>36</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> DRP003977 Illumina HiSeq 3000 sequencing of SAMD00182242       1279740096.0 35548336.0 DRR189399 0:36 A:314850837;C:294188093;G:304654881;T:365946483;N:99802 36       314850837 294188093 304654881 365946483 99802 DRX179864 DRS200400 DRA008864 NIG|National Institute of Genetics (Japan) National Institute of Genetics (Japan) 1 0.89485   0.18205   0.70593   0.49333   36   B   usable mapping rate illumina hiseq_era unknown poly_a unknown bulk unknown unknown   Japan 2021-08-08 Adult Adult Brain Nervous System
106 106 DRR189398 DRX179863 DRS200399 DRP003977 PRJDB4470 Gene expression analysis of the zebrafish brain DRP003977 Other Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.       Telencephalon of adult zebrafrish 30 min post test session of 1 day memory test in non trace 2 Way Active Avoidance coditioning 1 SAMD00182241   sample name:CSUS Tel 30 1d memory 1|genotype:wild type|tissue:brain                 Illumina HiSeq 3000 sequencing of SAMD00182241 DRX179863 CSUS Tel 30 1d memory 1 1 SureSelect Strand Specific RNA Library Prep Kit   RNA-Seq TRANSCRIPTOMIC PolyA SINGLE ILLUMINA Illumina HiSeq 3000 <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>36</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> DRP003977 Illumina HiSeq 3000 sequencing of SAMD00182241       3012353100.0 83676475.0 DRR189398 0:36 A:740795057;C:693120664;G:717419185;T:860784745;N:233449 36       740795057 693120664 717419185 860784745 233449 DRX179863 DRS200399 DRA008864 NIG|National Institute of Genetics (Japan) National Institute of Genetics (Japan) 1 0.89676   0.1791   0.70569   0.49835   36   B   usable mapping rate illumina hiseq_era unknown poly_a unknown bulk unknown unknown   Japan 2021-08-08 Adult Adult Brain Nervous System
107 107 DRR189397 DRX179862 DRS200428 DRP003977 PRJDB4470 Gene expression analysis of the zebrafish brain DRP003977 Other Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.       Telencephalon of adult zebrafrish 60 min post exposure to the conditioning tank 3 SAMD00182240   sample name:Cont Tel 60 3|genotype:wild type|tissue:brain                 Illumina HiSeq 3000 sequencing of SAMD00182240 DRX179862 Cont Tel 60 3 1 SureSelect Strand Specific RNA Library Prep Kit   RNA-Seq TRANSCRIPTOMIC PolyA SINGLE ILLUMINA Illumina HiSeq 3000 <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>50</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> DRP003977 Illumina HiSeq 3000 sequencing of SAMD00182240       5590147750.0 111802955.0 DRR189397 0:50 A:1365131560;C:1269631295;G:1366791321;T:1588422020;N:171554 50       1365131560 1269631295 1366791321 1588422020 171554 DRX179862 DRS200428 DRA008863 NIG|National Institute of Genetics (Japan) National Institute of Genetics (Japan) 1 0.89334   0.16354   0.7011   0.49383   50   B   usable mapping rate illumina hiseq_era unknown poly_a unknown bulk unknown unknown   Japan 2021-08-08 Adult Adult Brain Nervous System
108 108 DRR189396 DRX179861 DRS200427 DRP003977 PRJDB4470 Gene expression analysis of the zebrafish brain DRP003977 Other Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.       Telencephalon of adult zebrafrish 60 min post exposure to the conditioning tank 2 SAMD00182239   sample name:Cont Tel 60 2|genotype:wild type|tissue:brain                 Illumina HiSeq 3000 sequencing of SAMD00182239 DRX179861 Cont Tel 60 2 1 SureSelect Strand Specific RNA Library Prep Kit   RNA-Seq TRANSCRIPTOMIC PolyA SINGLE ILLUMINA Illumina HiSeq 3000 <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>50</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> DRP003977 Illumina HiSeq 3000 sequencing of SAMD00182239       6429019650.0 128580393.0 DRR189396 0:50 A:1570141412;C:1457539089;G:1572785681;T:1828358007;N:195461 50       1570141412 1457539089 1572785681 1828358007 195461 DRX179861 DRS200427 DRA008863 NIG|National Institute of Genetics (Japan) National Institute of Genetics (Japan) 1 0.89595   0.16204   0.70743   0.49805   50   B   usable mapping rate illumina hiseq_era unknown poly_a unknown bulk unknown unknown   Japan 2021-08-08 Adult Adult Brain Nervous System
109 109 DRR189395 DRX179860 DRS200426 DRP003977 PRJDB4470 Gene expression analysis of the zebrafish brain DRP003977 Other Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.       Telencephalon of adult zebrafrish 60 min post exposure to the conditioning tank 1 SAMD00182238   sample name:Cont Tel 60 1|genotype:wild type|tissue:brain                 Illumina HiSeq 3000 sequencing of SAMD00182238 DRX179860 Cont Tel 60 1 1 SureSelect Strand Specific RNA Library Prep Kit   RNA-Seq TRANSCRIPTOMIC PolyA SINGLE ILLUMINA Illumina HiSeq 3000 <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>50</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> DRP003977 Illumina HiSeq 3000 sequencing of SAMD00182238       6644185850.0 132883717.0 DRR189395 0:50 A:1637322491;C:1498943556;G:1616212676;T:1891505597;N:201530 50       1637322491 1498943556 1616212676 1891505597 201530 DRX179860 DRS200426 DRA008863 NIG|National Institute of Genetics (Japan) National Institute of Genetics (Japan) 1 0.89573   0.16421   0.7037   0.49805   50   B   usable mapping rate illumina hiseq_era unknown poly_a unknown bulk unknown unknown   Japan 2021-08-08 Adult Adult Brain Nervous System
110 110 DRR189394 DRX179859 DRS200407 DRP003977 PRJDB4470 Gene expression analysis of the zebrafish brain DRP003977 Other Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.       Telencephalon of adult zebrafrish 60 min post light stimulation in the conditioning tank 3 SAMD00182237   sample name:CS Tel 60 3|genotype:wild type|tissue:brain                 Illumina HiSeq 3000 sequencing of SAMD00182237 DRX179859 CS Tel 60 3 1 SureSelect Strand Specific RNA Library Prep Kit   RNA-Seq TRANSCRIPTOMIC PolyA SINGLE ILLUMINA Illumina HiSeq 3000 <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>36</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> DRP003977 Illumina HiSeq 3000 sequencing of SAMD00182237       1387078956.0 38529971.0 DRR189394 0:36 A:333990578;C:315361737;G:337599372;T:400057968;N:69301 36       333990578 315361737 337599372 400057968 69301 DRX179859 DRS200407 DRA008862 NIG|National Institute of Genetics (Japan) National Institute of Genetics (Japan) 1 0.88133   0.18418   0.70747   0.4971   36   B   usable mapping rate illumina hiseq_era unknown poly_a unknown bulk unknown unknown   Japan 2021-08-08 Adult Adult Brain Nervous System
111 111 DRR189393 DRX179858 DRS200406 DRP003977 PRJDB4470 Gene expression analysis of the zebrafish brain DRP003977 Other Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.       Telencephalon of adult zebrafrish 60 min post light stimulation in the conditioning tank 2 SAMD00182236   sample name:CS Tel 60 2|genotype:wild type|tissue:brain                 Illumina HiSeq 3000 sequencing of SAMD00182236 DRX179858 CS Tel 60 2 1 SureSelect Strand Specific RNA Library Prep Kit   RNA-Seq TRANSCRIPTOMIC PolyA SINGLE ILLUMINA Illumina HiSeq 3000 <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>36</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> DRP003977 Illumina HiSeq 3000 sequencing of SAMD00182236       537029424.0 14917484.0 DRR189393 0:36 A:127421487;C:122494467;G:132164579;T:154921150;N:27741 36       127421487 122494467 132164579 154921150 27741 DRX179858 DRS200406 DRA008862 NIG|National Institute of Genetics (Japan) National Institute of Genetics (Japan) 1 0.88264   0.17793   0.7083   0.49123   36   B   usable mapping rate illumina hiseq_era unknown poly_a unknown bulk unknown unknown   Japan 2021-08-08 Adult Adult Brain Nervous System
112 112 DRR189392 DRX179857 DRS200405 DRP003977 PRJDB4470 Gene expression analysis of the zebrafish brain DRP003977 Other Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.       Telencephalon of adult zebrafrish 60 min post light stimulation in the conditioning tank 1 SAMD00182235   sample name:CS Tel 60 1|genotype:wild type|tissue:brain                 Illumina HiSeq 3000 sequencing of SAMD00182235 DRX179857 CS Tel 60 1 1 SureSelect Strand Specific RNA Library Prep Kit   RNA-Seq TRANSCRIPTOMIC PolyA SINGLE ILLUMINA Illumina HiSeq 3000 <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>36</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> DRP003977 Illumina HiSeq 3000 sequencing of SAMD00182235       3163663656.0 87879546.0 DRR189392 0:36 A:766146692;C:714108562;G:771785405;T:911468887;N:154110 36       766146692 714108562 771785405 911468887 154110 DRX179857 DRS200405 DRA008862 NIG|National Institute of Genetics (Japan) National Institute of Genetics (Japan) 1 0.88114   0.1803   0.7052   0.4917   36   B   usable mapping rate illumina hiseq_era unknown poly_a unknown bulk unknown unknown   Japan 2021-08-08 Adult Adult Brain Nervous System
113 113 DRR189391 DRX179856 DRS200404 DRP003977 PRJDB4470 Gene expression analysis of the zebrafish brain DRP003977 Other Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.       Telencephalon of adult zebrafrish 60 min post electrical shock delivery in the conditioning tank 3 SAMD00182234   sample name:US Tel 60 3|genotype:wild type|tissue:brain                 Illumina HiSeq 3000 sequencing of SAMD00182234 DRX179856 US Tel 60 3 1 SureSelect Strand Specific RNA Library Prep Kit   RNA-Seq TRANSCRIPTOMIC PolyA SINGLE ILLUMINA Illumina HiSeq 3000 <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>36</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> DRP003977 Illumina HiSeq 3000 sequencing of SAMD00182234       1535884704.0 42663464.0 DRR189391 0:36 A:367444721;C:350978310;G:375524359;T:441859454;N:77860 36       367444721 350978310 375524359 441859454 77860 DRX179856 DRS200404 DRA008861 NIG|National Institute of Genetics (Japan) National Institute of Genetics (Japan) 1 0.88103   0.1788   0.7082   0.49462   36   B   usable mapping rate illumina hiseq_era unknown poly_a unknown bulk unknown unknown   Japan 2021-08-08 Adult Adult Brain Nervous System
114 114 DRR189390 DRX179855 DRS200403 DRP003977 PRJDB4470 Gene expression analysis of the zebrafish brain DRP003977 Other Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.       Telencephalon of adult zebrafrish 60 min post electrical shock delivery in the conditioning tank 2 SAMD00182233   sample name:US Tel 60 2|genotype:wild type|tissue:brain                 Illumina HiSeq 3000 sequencing of SAMD00182233 DRX179855 US Tel 60 2 1 SureSelect Strand Specific RNA Library Prep Kit   RNA-Seq TRANSCRIPTOMIC PolyA SINGLE ILLUMINA Illumina HiSeq 3000 <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>36</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> DRP003977 Illumina HiSeq 3000 sequencing of SAMD00182233       737309916.0 20480831.0 DRR189390 0:36 A:176982713;C:168284406;G:179343270;T:212662841;N:36686 36       176982713 168284406 179343270 212662841 36686 DRX179855 DRS200403 DRA008861 NIG|National Institute of Genetics (Japan) National Institute of Genetics (Japan) 1 0.87995   0.18515   0.70816   0.49393   36   B   usable mapping rate illumina hiseq_era unknown poly_a unknown bulk unknown unknown   Japan 2021-08-08 Adult Adult Brain Nervous System
115 115 DRR189389 DRX179854 DRS200402 DRP003977 PRJDB4470 Gene expression analysis of the zebrafish brain DRP003977 Other Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.       Telencephalon of adult zebrafrish 60 min post electrical shock delivery in the conditioning tank 1 SAMD00182232   sample name:US Tel 60 1|genotype:wild type|tissue:brain                 Illumina HiSeq 3000 sequencing of SAMD00182232 DRX179854 US Tel 60 1 1 SureSelect Strand Specific RNA Library Prep Kit   RNA-Seq TRANSCRIPTOMIC PolyA SINGLE ILLUMINA Illumina HiSeq 3000 <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>36</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> DRP003977 Illumina HiSeq 3000 sequencing of SAMD00182232       595837404.0 16551039.0 DRR189389 0:36 A:143956004;C:134783754;G:145443580;T:171624939;N:29127 36       143956004 134783754 145443580 171624939 29127 DRX179854 DRS200402 DRA008861 NIG|National Institute of Genetics (Japan) National Institute of Genetics (Japan) 1 0.87682   0.18328   0.70309   0.49081   36   B   usable mapping rate illumina hiseq_era unknown poly_a unknown bulk unknown unknown   Japan 2021-08-08 Adult Adult Brain Nervous System
116 116 DRR189388 DRX179853 DRS200452 DRP003977 PRJDB4470 Gene expression analysis of the zebrafish brain DRP003977 Other Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.       Telencephalon of adult zebrafrish 60 min post light and electrical shock association in non trace 2 Way Active Avoidance coditioning 3 SAMD00182231   sample name:CSUS Tel 60 3|genotype:wild type|tissue:brain                 Illumina HiSeq 3000 sequencing of SAMD00182231 DRX179853 CSUS Tel 60 3 1 SureSelect Strand Specific RNA Library Prep Kit   RNA-Seq TRANSCRIPTOMIC PolyA SINGLE ILLUMINA Illumina HiSeq 3000 <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>36</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> DRP003977 Illumina HiSeq 3000 sequencing of SAMD00182231       608050044.0 16890279.0 DRR189388 0:36 A:145025778;C:137906052;G:149542672;T:175545338;N:30204 36       145025778 137906052 149542672 175545338 30204 DRX179853 DRS200452 DRA008860 NIG|National Institute of Genetics (Japan) National Institute of Genetics (Japan) 1 0.87983   0.18602   0.70445   0.49195   36   B   usable mapping rate illumina hiseq_era unknown poly_a unknown bulk unknown unknown   Japan 2021-08-08 Adult Adult Brain Nervous System
117 117 DRR189387 DRX179852 DRS200451 DRP003977 PRJDB4470 Gene expression analysis of the zebrafish brain DRP003977 Other Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.       Telencephalon of adult zebrafrish 60 min post light and electrical shock association in non trace 2 Way Active Avoidance coditioning 2 SAMD00182230   sample name:CSUS Tel 60 2|genotype:wild type|tissue:brain                 Illumina HiSeq 3000 sequencing of SAMD00182230 DRX179852 CSUS Tel 60 2 1 SureSelect Strand Specific RNA Library Prep Kit   RNA-Seq TRANSCRIPTOMIC PolyA SINGLE ILLUMINA Illumina HiSeq 3000 <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>36</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> DRP003977 Illumina HiSeq 3000 sequencing of SAMD00182230       777589452.0 21599707.0 DRR189387 0:36 A:185463269;C:177742981;G:190443063;T:223900096;N:40043 36       185463269 177742981 190443063 223900096 40043 DRX179852 DRS200451 DRA008860 NIG|National Institute of Genetics (Japan) National Institute of Genetics (Japan) 1 0.88331   0.17918   0.70516   0.48956   36   B   usable mapping rate illumina hiseq_era unknown poly_a unknown bulk unknown unknown   Japan 2021-08-08 Adult Adult Brain Nervous System
118 118 DRR189386 DRX179851 DRS200450 DRP003977 PRJDB4470 Gene expression analysis of the zebrafish brain DRP003977 Other Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.       Telencephalon of adult zebrafrish 60 min post light and electrical shock association in non trace 2 Way Active Avoidance coditioning 1 SAMD00182229   sample name:CSUS Tel 60 1|genotype:wild type|tissue:brain                 Illumina HiSeq 3000 sequencing of SAMD00182229 DRX179851 CSUS Tel 60 1 1 SureSelect Strand Specific RNA Library Prep Kit   RNA-Seq TRANSCRIPTOMIC PolyA SINGLE ILLUMINA Illumina HiSeq 3000 <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>36</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> DRP003977 Illumina HiSeq 3000 sequencing of SAMD00182229       2738622348.0 76072843.0 DRR189386 0:36 A:662989485;C:622023190;G:663013857;T:790459930;N:135886 36       662989485 622023190 663013857 790459930 135886 DRX179851 DRS200450 DRA008860 NIG|National Institute of Genetics (Japan) National Institute of Genetics (Japan) 1 0.87743   0.19164   0.70025   0.49073   36   B   usable mapping rate illumina hiseq_era unknown poly_a unknown bulk unknown unknown   Japan 2021-08-08 Adult Adult Brain Nervous System
119 119 DRR189385 DRX179850 DRS200435 DRP003977 PRJDB4470 Gene expression analysis of the zebrafish brain DRP003977 Other Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.       Telencephalon of EMX3 / adult zebrafish 3 SAMD00182228   sample name:Emx3 Adult Tel 3|genotype:Emx3 / |tissue:brain                 Illumina HiSeq 3000 sequencing of SAMD00182228 DRX179850 Emx3 / Adult Tel 3 1 SureSelect Strand Specific RNA Library Prep Kit   RNA-Seq TRANSCRIPTOMIC PolyA SINGLE ILLUMINA Illumina HiSeq 3000 <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>36</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> DRP003977 Illumina HiSeq 3000 sequencing of SAMD00182228       1323636552.0 36767682.0 DRR189385 0:36 A:309582925;C:310206951;G:325673647;T:378136039;N:36990 36       309582925 310206951 325673647 378136039 36990 DRX179850 DRS200435 DRA008859 NIG|National Institute of Genetics (Japan) National Institute of Genetics (Japan) 1 0.8932   0.19244   0.71334   0.50591   36   B   usable mapping rate illumina hiseq_era unknown poly_a unknown bulk unknown unknown   Japan 2021-08-08 Adult Adult Brain Nervous System
120 120 DRR189384 DRX179849 DRS200434 DRP003977 PRJDB4470 Gene expression analysis of the zebrafish brain DRP003977 Other Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.       Telencephalon of EMX3 / adult zebrafish 2 SAMD00182227   sample name:Emx3 Adult Tel 2|genotype:Emx3 / |tissue:brain                 Illumina HiSeq 3000 sequencing of SAMD00182227 DRX179849 Emx3 / Adult Tel 2 1 SureSelect Strand Specific RNA Library Prep Kit   RNA-Seq TRANSCRIPTOMIC PolyA SINGLE ILLUMINA Illumina HiSeq 3000 <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>36</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> DRP003977 Illumina HiSeq 3000 sequencing of SAMD00182227       2994105168.0 83169588.0 DRR189384 0:36 A:709730692;C:691531995;G:727245229;T:865514929;N:82323 36       709730692 691531995 727245229 865514929 82323 DRX179849 DRS200434 DRA008859 NIG|National Institute of Genetics (Japan) National Institute of Genetics (Japan) 1 0.90382   0.1826   0.70197   0.49719   36   B   usable mapping rate illumina hiseq_era unknown poly_a unknown bulk unknown unknown   Japan 2021-08-08 Adult Adult Brain Nervous System
121 121 DRR189383 DRX179848 DRS200433 DRP003977 PRJDB4470 Gene expression analysis of the zebrafish brain DRP003977 Other Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.       Telencephalon of EMX3 / adult zebrafish 1 SAMD00182226   sample name:Emx3 Adult Tel 1|genotype:Emx3 / |tissue:brain                 Illumina HiSeq 3000 sequencing of SAMD00182226 DRX179848 Emx3 / Adult Tel 1 1 SureSelect Strand Specific RNA Library Prep Kit   RNA-Seq TRANSCRIPTOMIC PolyA SINGLE ILLUMINA Illumina HiSeq 3000 <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>36</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> DRP003977 Illumina HiSeq 3000 sequencing of SAMD00182226       1151464896.0 31985136.0 DRR189383 0:36 A:272427216;C:266708394;G:280781178;T:331515997;N:32111 36       272427216 266708394 280781178 331515997 32111 DRX179848 DRS200433 DRA008859 NIG|National Institute of Genetics (Japan) National Institute of Genetics (Japan) 1 0.89942   0.17402   0.70364   0.49438   36   B   usable mapping rate illumina hiseq_era unknown poly_a unknown bulk unknown unknown   Japan 2021-08-08 Adult Adult Brain Nervous System
122 122 DRR189382 DRX179847 DRS200421 DRP003977 PRJDB4470 Gene expression analysis of the zebrafish brain DRP003977 Other Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.       Telencephalon of wild type adult zebrafish 3 SAMD00182225   sample name:WT Adult Tel 3|genotype:wild type|tissue:brain                 Illumina HiSeq 3000 sequencing of SAMD00182225 DRX179847 WT Adult Tel 3 1 SureSelect Strand Specific RNA Library Prep Kit   RNA-Seq TRANSCRIPTOMIC PolyA SINGLE ILLUMINA Illumina HiSeq 3000 <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>36</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> DRP003977 Illumina HiSeq 3000 sequencing of SAMD00182225       2837488824.0 78819134.0 DRR189382 0:36 A:678395895;C:655098137;G:687520693;T:816395518;N:78581 36       678395895 655098137 687520693 816395518 78581 DRX179847 DRS200421 DRA008858 NIG|National Institute of Genetics (Japan) National Institute of Genetics (Japan) 1 0.89483   0.18623   0.70544   0.49839   36   B   usable mapping rate illumina hiseq_era unknown poly_a unknown bulk unknown unknown   Japan 2021-08-08 Adult Adult Brain Nervous System
123 123 DRR189381 DRX179846 DRS200420 DRP003977 PRJDB4470 Gene expression analysis of the zebrafish brain DRP003977 Other Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.       Telencephalon of wild type adult zebrafish 2 SAMD00182224   sample name:WT Adult Tel 2|genotype:wild type|tissue:brain                 Illumina HiSeq 3000 sequencing of SAMD00182224 DRX179846 WT Adult Tel 2 1 SureSelect Strand Specific RNA Library Prep Kit   RNA-Seq TRANSCRIPTOMIC PolyA SINGLE ILLUMINA Illumina HiSeq 3000 <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>36</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> DRP003977 Illumina HiSeq 3000 sequencing of SAMD00182224       1456800264.0 40466674.0 DRR189381 0:36 A:345320431;C:336838149;G:356264798;T:418336552;N:40334 36       345320431 336838149 356264798 418336552 40334 DRX179846 DRS200420 DRA008858 NIG|National Institute of Genetics (Japan) National Institute of Genetics (Japan) 1 0.89496   0.18691   0.70802   0.49721   36   B   usable mapping rate illumina hiseq_era unknown poly_a unknown bulk unknown unknown   Japan 2021-08-08 Adult Adult Brain Nervous System
124 124 DRR189380 DRX179845 DRS200419 DRP003977 PRJDB4470 Gene expression analysis of the zebrafish brain DRP003977 Other Gene expression profiling by RNA seq of specific regions and subpopulations of neurons in the zebrafish brain that control behaviors.       Telencephalon of wild type adult zebrafish 1 SAMD00182223   sample name:WT Adult Tel 1|genotype:wild type|tissue:brain                 Illumina HiSeq 3000 sequencing of SAMD00182223 DRX179845 WT Adult Tel 1 1 SureSelect Strand Specific RNA Library Prep Kit   RNA-Seq TRANSCRIPTOMIC PolyA SINGLE ILLUMINA Illumina HiSeq 3000 <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>36</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> DRP003977 Illumina HiSeq 3000 sequencing of SAMD00182223       2533282452.0 70368957.0 DRR189380 0:36 A:602595083;C:585466052;G:616064286;T:729086926;N:70105 36       602595083 585466052 616064286 729086926 70105 DRX179845 DRS200419 DRA008858 NIG|National Institute of Genetics (Japan) National Institute of Genetics (Japan) 1 0.89412   0.19005   0.70816   0.49843   36   B   usable mapping rate illumina hiseq_era unknown poly_a unknown bulk unknown unknown   Japan 2021-08-08 Adult Adult Brain Nervous System
10407 10407 ERR9077202 ERX8620336 ERS10857224 ERP135966 PRJEB51339 RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae E-MTAB-11505 Transcriptome Analysis Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21   Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. Sv2aWT5S8 SAMEA13254181 Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254181|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aWT5S8|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/+|individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aWT5S8|sex:not available|strain:AB                 NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae E MTAB 11505:Sv2aWT5S8 s Sv2aWT5S8 s RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. Experimental Factor: genotype:sv2a+/+ RNA-Seq TRANSCRIPTOMIC PolyA SINGLE ILLUMINA NextSeq 500   ERP135966 NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 sv2aWT5S8L001-1.fastq.gz fastq 652046291.0 8633105.0 E MTAB 11505:sv2aWT5S8L001 0:75.53 1:0 A:159570243;C:156420495;G:154853315;T:181073030;N:129208 75 0     159570243 156420495 154853315 181073030 129208 ERX8620336 ERS10857224 ERA9644804 Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive 1 0.95245   0.1148   0.65756   0.47011   75   B   usable mapping rate illumina nextseq unknown poly_a trueseq bulk unknown unknown   Belgium 2022-04-21 Larval Larval Head Nervous System
10408 10408 ERR9077203 ERX8620336 ERS10857224 ERP135966 PRJEB51339 RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae E-MTAB-11505 Transcriptome Analysis Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21   Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. Sv2aWT5S8 SAMEA13254181 Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254181|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aWT5S8|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/+|individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aWT5S8|sex:not available|strain:AB                 NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae E MTAB 11505:Sv2aWT5S8 s Sv2aWT5S8 s RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. Experimental Factor: genotype:sv2a+/+ RNA-Seq TRANSCRIPTOMIC PolyA SINGLE ILLUMINA NextSeq 500   ERP135966 NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 sv2aWT5S8L002-1.fastq.gz fastq 653840525.0 8656886.0 E MTAB 11505:sv2aWT5S8L002 0:75.53 1:0 A:159902458;C:156826842;G:155509070;T:181475104;N:127051 75 0     159902458 156826842 155509070 181475104 127051 ERX8620336 ERS10857224 ERA9644804 Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive 1 0.95226   0.1156   0.65792   0.4667   76   B   usable mapping rate illumina nextseq unknown poly_a trueseq bulk unknown unknown   Belgium 2022-04-21 Larval Larval Head Nervous System
10409 10409 ERR9077204 ERX8620336 ERS10857224 ERP135966 PRJEB51339 RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae E-MTAB-11505 Transcriptome Analysis Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21   Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. Sv2aWT5S8 SAMEA13254181 Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254181|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aWT5S8|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/+|individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aWT5S8|sex:not available|strain:AB                 NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae E MTAB 11505:Sv2aWT5S8 s Sv2aWT5S8 s RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. Experimental Factor: genotype:sv2a+/+ RNA-Seq TRANSCRIPTOMIC PolyA SINGLE ILLUMINA NextSeq 500   ERP135966 NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 sv2aWT5S8L003-1.fastq.gz fastq 659584196.0 8732885.0 E MTAB 11505:sv2aWT5S8L003 0:75.53 1:0 A:161257146;C:158299734;G:156793443;T:183128972;N:104901 75 0     161257146 158299734 156793443 183128972 104901 ERX8620336 ERS10857224 ERA9644804 Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive 1 0.95298   0.11485   0.65798   0.46853   76   B   usable mapping rate illumina nextseq unknown poly_a trueseq bulk unknown unknown   Belgium 2022-04-21 Larval Larval Head Nervous System
10410 10410 ERR9077205 ERX8620336 ERS10857224 ERP135966 PRJEB51339 RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae E-MTAB-11505 Transcriptome Analysis Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21   Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. Sv2aWT5S8 SAMEA13254181 Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254181|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aWT5S8|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/+|individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aWT5S8|sex:not available|strain:AB                 NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae E MTAB 11505:Sv2aWT5S8 s Sv2aWT5S8 s RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. Experimental Factor: genotype:sv2a+/+ RNA-Seq TRANSCRIPTOMIC PolyA SINGLE ILLUMINA NextSeq 500   ERP135966 NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 sv2aWT5S8L004-1.fastq.gz fastq 666627746.0 8826123.0 E MTAB 11505:sv2aWT5S8L004 0:75.53 1:0 A:162975131;C:159937258;G:158506242;T:185106149;N:102966 75 0     162975131 159937258 158506242 185106149 102966 ERX8620336 ERS10857224 ERA9644804 Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive 1 0.9531   0.11425   0.65736   0.47309   76   B   usable mapping rate illumina nextseq unknown poly_a trueseq bulk unknown unknown   Belgium 2022-04-21 Larval Larval Head Nervous System
10411 10411 ERR9077198 ERX8620335 ERS10857223 ERP135966 PRJEB51339 RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae E-MTAB-11505 Transcriptome Analysis Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21   Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. Sv2aWT4S7 SAMEA13254180 Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254180|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aWT4S7|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/+|individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aWT4S7|sex:not available|strain:AB                 NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae E MTAB 11505:Sv2aWT4S7 s Sv2aWT4S7 s RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. Experimental Factor: genotype:sv2a+/+ RNA-Seq TRANSCRIPTOMIC PolyA SINGLE ILLUMINA NextSeq 500   ERP135966 NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 sv2aWT4S7L001-1.fastq.gz fastq 683751258.0 9051795.0 E MTAB 11505:sv2aWT4S7L001 0:75.54 1:0 A:162889923;C:167127644;G:164779384;T:188822907;N:131400 75 0     162889923 167127644 164779384 188822907 131400 ERX8620335 ERS10857223 ERA9644804 Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive 1 0.95739   0.0959   0.6606   0.46826   75   B   usable mapping rate illumina nextseq unknown poly_a trueseq bulk unknown unknown   Belgium 2022-04-21 Larval Larval Head Nervous System
10412 10412 ERR9077199 ERX8620335 ERS10857223 ERP135966 PRJEB51339 RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae E-MTAB-11505 Transcriptome Analysis Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21   Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. Sv2aWT4S7 SAMEA13254180 Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254180|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aWT4S7|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/+|individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aWT4S7|sex:not available|strain:AB                 NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae E MTAB 11505:Sv2aWT4S7 s Sv2aWT4S7 s RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. Experimental Factor: genotype:sv2a+/+ RNA-Seq TRANSCRIPTOMIC PolyA SINGLE ILLUMINA NextSeq 500   ERP135966 NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 sv2aWT4S7L002-1.fastq.gz fastq 684187711.0 9057591.0 E MTAB 11505:sv2aWT4S7L002 0:75.54 1:0 A:162923879;C:167246213;G:165071145;T:188818774;N:127700 75 0     162923879 167246213 165071145 188818774 127700 ERX8620335 ERS10857223 ERA9644804 Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive 1 0.95724   0.0959   0.66036   0.47026   76   B   usable mapping rate illumina nextseq unknown poly_a trueseq bulk unknown unknown   Belgium 2022-04-21 Larval Larval Head Nervous System
10413 10413 ERR9077200 ERX8620335 ERS10857223 ERP135966 PRJEB51339 RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae E-MTAB-11505 Transcriptome Analysis Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21   Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. Sv2aWT4S7 SAMEA13254180 Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254180|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aWT4S7|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/+|individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aWT4S7|sex:not available|strain:AB                 NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae E MTAB 11505:Sv2aWT4S7 s Sv2aWT4S7 s RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. Experimental Factor: genotype:sv2a+/+ RNA-Seq TRANSCRIPTOMIC PolyA SINGLE ILLUMINA NextSeq 500   ERP135966 NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 sv2aWT4S7L003-1.fastq.gz fastq 692320213.0 9165132.0 E MTAB 11505:sv2aWT4S7L003 0:75.54 1:0 A:164772792;C:169329920;G:166952052;T:191164422;N:101027 75 0     164772792 169329920 166952052 191164422 101027 ERX8620335 ERS10857223 ERA9644804 Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive 1 0.95786   0.09701   0.66131   0.47564   76   B   usable mapping rate illumina nextseq unknown poly_a trueseq bulk unknown unknown   Belgium 2022-04-21 Larval Larval Head Nervous System
10414 10414 ERR9077201 ERX8620335 ERS10857223 ERP135966 PRJEB51339 RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae E-MTAB-11505 Transcriptome Analysis Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21   Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. Sv2aWT4S7 SAMEA13254180 Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254180|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aWT4S7|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/+|individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aWT4S7|sex:not available|strain:AB                 NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae E MTAB 11505:Sv2aWT4S7 s Sv2aWT4S7 s RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. Experimental Factor: genotype:sv2a+/+ RNA-Seq TRANSCRIPTOMIC PolyA SINGLE ILLUMINA NextSeq 500   ERP135966 NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 sv2aWT4S7L004-1.fastq.gz fastq 697047881.0 9227702.0 E MTAB 11505:sv2aWT4S7L004 0:75.54 1:0 A:165881728;C:170388393;G:168105900;T:192572068;N:99792 75 0     165881728 170388393 168105900 192572068 99792 ERX8620335 ERS10857223 ERA9644804 Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive 1 0.9584   0.09565   0.66115   0.46967   76   B   usable mapping rate illumina nextseq unknown poly_a trueseq bulk unknown unknown   Belgium 2022-04-21 Larval Larval Head Nervous System
10415 10415 ERR9077194 ERX8620334 ERS10857222 ERP135966 PRJEB51339 RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae E-MTAB-11505 Transcriptome Analysis Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21   Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. Sv2aWT3S2 SAMEA13254179 Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254179|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aWT3S2|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/+|individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aWT3S2|sex:not available|strain:AB                 NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae E MTAB 11505:Sv2aWT3S2 s Sv2aWT3S2 s RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. Experimental Factor: genotype:sv2a+/+ RNA-Seq TRANSCRIPTOMIC PolyA SINGLE ILLUMINA NextSeq 500   ERP135966 NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 sv2aWT3S2L001-1.fastq.gz fastq 723508205.0 9579193.0 E MTAB 11505:sv2aWT3S2L001 0:75.53 1:0 A:177832489;C:174193135;G:169978598;T:201360184;N:143799 75 0     177832489 174193135 169978598 201360184 143799 ERX8620334 ERS10857222 ERA9644804 Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive 1 0.95218   0.11732   0.65916   0.47955   71   B   usable mapping rate illumina nextseq unknown poly_a trueseq bulk unknown unknown   Belgium 2022-04-21 Larval Larval Head Nervous System
10416 10416 ERR9077195 ERX8620334 ERS10857222 ERP135966 PRJEB51339 RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae E-MTAB-11505 Transcriptome Analysis Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21   Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. Sv2aWT3S2 SAMEA13254179 Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254179|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aWT3S2|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/+|individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aWT3S2|sex:not available|strain:AB                 NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae E MTAB 11505:Sv2aWT3S2 s Sv2aWT3S2 s RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. Experimental Factor: genotype:sv2a+/+ RNA-Seq TRANSCRIPTOMIC PolyA SINGLE ILLUMINA NextSeq 500   ERP135966 NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 sv2aWT3S2L002-1.fastq.gz fastq 726190024.0 9614674.0 E MTAB 11505:sv2aWT3S2L002 0:75.53 1:0 A:178408224;C:174776263;G:170820745;T:202047224;N:137568 75 0     178408224 174776263 170820745 202047224 137568 ERX8620334 ERS10857222 ERA9644804 Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive 1 0.95093   0.11567   0.65865   0.46877   75   B   usable mapping rate illumina nextseq unknown poly_a trueseq bulk unknown unknown   Belgium 2022-04-21 Larval Larval Head Nervous System
10417 10417 ERR9077196 ERX8620334 ERS10857222 ERP135966 PRJEB51339 RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae E-MTAB-11505 Transcriptome Analysis Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21   Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. Sv2aWT3S2 SAMEA13254179 Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254179|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aWT3S2|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/+|individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aWT3S2|sex:not available|strain:AB                 NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae E MTAB 11505:Sv2aWT3S2 s Sv2aWT3S2 s RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. Experimental Factor: genotype:sv2a+/+ RNA-Seq TRANSCRIPTOMIC PolyA SINGLE ILLUMINA NextSeq 500   ERP135966 NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 sv2aWT3S2L003-1.fastq.gz fastq 735499955.0 9737845.0 E MTAB 11505:sv2aWT3S2L003 0:75.53 1:0 A:180659962;C:177141362;G:172905323;T:204678308;N:115000 75 0     180659962 177141362 172905323 204678308 115000 ERX8620334 ERS10857222 ERA9644804 Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive 1 0.95233   0.11614   0.65898   0.47114   76   B   usable mapping rate illumina nextseq unknown poly_a trueseq bulk unknown unknown   Belgium 2022-04-21 Larval Larval Head Nervous System
10418 10418 ERR9077197 ERX8620334 ERS10857222 ERP135966 PRJEB51339 RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae E-MTAB-11505 Transcriptome Analysis Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21   Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. Sv2aWT3S2 SAMEA13254179 Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254179|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aWT3S2|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/+|individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aWT3S2|sex:not available|strain:AB                 NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae E MTAB 11505:Sv2aWT3S2 s Sv2aWT3S2 s RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. Experimental Factor: genotype:sv2a+/+ RNA-Seq TRANSCRIPTOMIC PolyA SINGLE ILLUMINA NextSeq 500   ERP135966 NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 sv2aWT3S2L004-1.fastq.gz fastq 745285512.0 9867337.0 E MTAB 11505:sv2aWT3S2L004 0:75.53 1:0 A:183067487;C:179408382;G:175250814;T:207445912;N:112917 75 0     183067487 179408382 175250814 207445912 112917 ERX8620334 ERS10857222 ERA9644804 Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive 1 0.95245   0.11655   0.65857   0.47087   76   B   usable mapping rate illumina nextseq unknown poly_a trueseq bulk unknown unknown   Belgium 2022-04-21 Larval Larval Head Nervous System
10419 10419 ERR9077190 ERX8620333 ERS10857221 ERP135966 PRJEB51339 RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae E-MTAB-11505 Transcriptome Analysis Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21   Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. Sv2aWT1S1 SAMEA13254178 Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254178|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aWT1S1|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/+|individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aWT1S1|sex:not available|strain:AB                 NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae E MTAB 11505:Sv2aWT1S1 s Sv2aWT1S1 s RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. Experimental Factor: genotype:sv2a+/+ RNA-Seq TRANSCRIPTOMIC PolyA SINGLE ILLUMINA NextSeq 500   ERP135966 NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 sv2aWT1S1L001-1.fastq.gz fastq 647414748.0 8571883.0 E MTAB 11505:sv2aWT1S1L001 0:75.53 1:0 A:154593859;C:160498057;G:153809612;T:178389112;N:124108 75 0     154593859 160498057 153809612 178389112 124108 ERX8620333 ERS10857221 ERA9644804 Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive 1 0.95955   0.08686   0.65955   0.47406   76   B   usable mapping rate illumina nextseq unknown poly_a trueseq bulk unknown unknown   Belgium 2022-04-21 Larval Larval Head Nervous System
10420 10420 ERR9077191 ERX8620333 ERS10857221 ERP135966 PRJEB51339 RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae E-MTAB-11505 Transcriptome Analysis Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21   Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. Sv2aWT1S1 SAMEA13254178 Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254178|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aWT1S1|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/+|individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aWT1S1|sex:not available|strain:AB                 NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae E MTAB 11505:Sv2aWT1S1 s Sv2aWT1S1 s RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. Experimental Factor: genotype:sv2a+/+ RNA-Seq TRANSCRIPTOMIC PolyA SINGLE ILLUMINA NextSeq 500   ERP135966 NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 sv2aWT1S1L002-1.fastq.gz fastq 646657758.0 8561861.0 E MTAB 11505:sv2aWT1S1L002 0:75.53 1:0 A:154348044;C:160294625;G:153804048;T:178092212;N:118829 75 0     154348044 160294625 153804048 178092212 118829 ERX8620333 ERS10857221 ERA9644804 Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive 1 0.95942   0.08632   0.65991   0.47539   76   B   usable mapping rate illumina nextseq unknown poly_a trueseq bulk unknown unknown   Belgium 2022-04-21 Larval Larval Head Nervous System
10421 10421 ERR9077192 ERX8620333 ERS10857221 ERP135966 PRJEB51339 RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae E-MTAB-11505 Transcriptome Analysis Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21   Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. Sv2aWT1S1 SAMEA13254178 Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254178|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aWT1S1|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/+|individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aWT1S1|sex:not available|strain:AB                 NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae E MTAB 11505:Sv2aWT1S1 s Sv2aWT1S1 s RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. Experimental Factor: genotype:sv2a+/+ RNA-Seq TRANSCRIPTOMIC PolyA SINGLE ILLUMINA NextSeq 500   ERP135966 NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 sv2aWT1S1L003-1.fastq.gz fastq 654781451.0 8669333.0 E MTAB 11505:sv2aWT1S1L003 0:75.53 1:0 A:156252744;C:162396721;G:155681450;T:180352911;N:97625 75 0     156252744 162396721 155681450 180352911 97625 ERX8620333 ERS10857221 ERA9644804 Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive 1 0.95972   0.08498   0.66137   0.47457   75   B   usable mapping rate illumina nextseq unknown poly_a trueseq bulk unknown unknown   Belgium 2022-04-21 Larval Larval Head Nervous System
10422 10422 ERR9077193 ERX8620333 ERS10857221 ERP135966 PRJEB51339 RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae E-MTAB-11505 Transcriptome Analysis Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21   Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. Sv2aWT1S1 SAMEA13254178 Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254178|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aWT1S1|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/+|individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aWT1S1|sex:not available|strain:AB                 NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae E MTAB 11505:Sv2aWT1S1 s Sv2aWT1S1 s RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. Experimental Factor: genotype:sv2a+/+ RNA-Seq TRANSCRIPTOMIC PolyA SINGLE ILLUMINA NextSeq 500   ERP135966 NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 sv2aWT1S1L004-1.fastq.gz fastq 659716937.0 8734618.0 E MTAB 11505:sv2aWT1S1L004 0:75.53 1:0 A:157367211;C:163585842;G:156911139;T:181757809;N:94936 75 0     157367211 163585842 156911139 181757809 94936 ERX8620333 ERS10857221 ERA9644804 Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive 1 0.95985   0.08585   0.65857   0.47349   76   B   usable mapping rate illumina nextseq unknown poly_a trueseq bulk unknown unknown   Belgium 2022-04-21 Larval Larval Head Nervous System
10423 10423 ERR9077186 ERX8620332 ERS10857220 ERP135966 PRJEB51339 RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae E-MTAB-11505 Transcriptome Analysis Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21   Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. Sv2aHO6S12 SAMEA13254177 Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254177|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHO6S12|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a / |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHO6S12|sex:not available|strain:AB                 NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae E MTAB 11505:Sv2aHO6S12 s Sv2aHO6S12 s RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. Experimental Factor: genotype:sv2a / RNA-Seq TRANSCRIPTOMIC PolyA SINGLE ILLUMINA NextSeq 500   ERP135966 NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 sv2aHO6S12L001-1.fastq.gz fastq 675018261.0 8937958.0 E MTAB 11505:sv2aHO6S12L001 0:75.52 1:0 A:166564263;C:160539328;G:160003980;T:187773509;N:137181 75 0     166564263 160539328 160003980 187773509 137181 ERX8620332 ERS10857220 ERA9644804 Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive 1 0.94964   0.13009   0.65413   0.47079   76   B   usable mapping rate illumina nextseq unknown poly_a trueseq bulk unknown unknown   Belgium 2022-04-21 Larval Larval Head Nervous System
10424 10424 ERR9077187 ERX8620332 ERS10857220 ERP135966 PRJEB51339 RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae E-MTAB-11505 Transcriptome Analysis Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21   Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. Sv2aHO6S12 SAMEA13254177 Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254177|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHO6S12|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a / |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHO6S12|sex:not available|strain:AB                 NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae E MTAB 11505:Sv2aHO6S12 s Sv2aHO6S12 s RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. Experimental Factor: genotype:sv2a / RNA-Seq TRANSCRIPTOMIC PolyA SINGLE ILLUMINA NextSeq 500   ERP135966 NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 sv2aHO6S12L002-1.fastq.gz fastq 672995667.0 8911161.0 E MTAB 11505:sv2aHO6S12L002 0:75.52 1:0 A:165990319;C:160039398;G:159722501;T:187108781;N:134668 75 0     165990319 160039398 159722501 187108781 134668 ERX8620332 ERS10857220 ERA9644804 Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive 1 0.94837   0.13029   0.65744   0.47959   76   B   usable mapping rate illumina nextseq unknown poly_a trueseq bulk unknown unknown   Belgium 2022-04-21 Larval Larval Head Nervous System
10425 10425 ERR9077188 ERX8620332 ERS10857220 ERP135966 PRJEB51339 RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae E-MTAB-11505 Transcriptome Analysis Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21   Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. Sv2aHO6S12 SAMEA13254177 Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254177|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHO6S12|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a / |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHO6S12|sex:not available|strain:AB                 NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae E MTAB 11505:Sv2aHO6S12 s Sv2aHO6S12 s RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. Experimental Factor: genotype:sv2a / RNA-Seq TRANSCRIPTOMIC PolyA SINGLE ILLUMINA NextSeq 500   ERP135966 NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 sv2aHO6S12L003-1.fastq.gz fastq 683276767.0 9047147.0 E MTAB 11505:sv2aHO6S12L003 0:75.52 1:0 A:168501690;C:162583852;G:162063190;T:190018001;N:110034 75 0     168501690 162583852 162063190 190018001 110034 ERX8620332 ERS10857220 ERA9644804 Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive 1 0.94983   0.13248   0.6565   0.47977   76   B   usable mapping rate illumina nextseq unknown poly_a trueseq bulk unknown unknown   Belgium 2022-04-21 Larval Larval Head Nervous System
10426 10426 ERR9077189 ERX8620332 ERS10857220 ERP135966 PRJEB51339 RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae E-MTAB-11505 Transcriptome Analysis Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21   Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. Sv2aHO6S12 SAMEA13254177 Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254177|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHO6S12|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a / |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHO6S12|sex:not available|strain:AB                 NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae E MTAB 11505:Sv2aHO6S12 s Sv2aHO6S12 s RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. Experimental Factor: genotype:sv2a / RNA-Seq TRANSCRIPTOMIC PolyA SINGLE ILLUMINA NextSeq 500   ERP135966 NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 sv2aHO6S12L004-1.fastq.gz fastq 686469910.0 9089431.0 E MTAB 11505:sv2aHO6S12L004 0:75.52 1:0 A:169287189;C:163276361;G:162844724;T:190954218;N:107418 75 0     169287189 163276361 162844724 190954218 107418 ERX8620332 ERS10857220 ERA9644804 Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive 1 0.95061   0.13029   0.65389   0.48044   76   B   usable mapping rate illumina nextseq unknown poly_a trueseq bulk unknown unknown   Belgium 2022-04-21 Larval Larval Head Nervous System
10427 10427 ERR9077182 ERX8620331 ERS10857219 ERP135966 PRJEB51339 RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae E-MTAB-11505 Transcriptome Analysis Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21   Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. Sv2aHO4S10 SAMEA13254176 Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254176|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHO4S10|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a / |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHO4S10|sex:not available|strain:AB                 NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae E MTAB 11505:Sv2aHO4S10 s Sv2aHO4S10 s RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. Experimental Factor: genotype:sv2a / RNA-Seq TRANSCRIPTOMIC PolyA SINGLE ILLUMINA NextSeq 500   ERP135966 NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 sv2aHO4S10L001-1.fastq.gz fastq 713858966.0 9452474.0 E MTAB 11505:sv2aHO4S10L001 0:75.52 1:0 A:177913695;C:169425317;G:167540012;T:198835532;N:144410 75 0     177913695 169425317 167540012 198835532 144410 ERX8620331 ERS10857219 ERA9644804 Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive 1 0.94848   0.12981   0.6636   0.47772   76   B   usable mapping rate illumina nextseq unknown poly_a trueseq bulk unknown unknown   Belgium 2022-04-21 Larval Larval Head Nervous System
10428 10428 ERR9077183 ERX8620331 ERS10857219 ERP135966 PRJEB51339 RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae E-MTAB-11505 Transcriptome Analysis Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21   Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. Sv2aHO4S10 SAMEA13254176 Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254176|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHO4S10|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a / |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHO4S10|sex:not available|strain:AB                 NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae E MTAB 11505:Sv2aHO4S10 s Sv2aHO4S10 s RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. Experimental Factor: genotype:sv2a / RNA-Seq TRANSCRIPTOMIC PolyA SINGLE ILLUMINA NextSeq 500   ERP135966 NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 sv2aHO4S10L002-1.fastq.gz fastq 717619593.0 9502301.0 E MTAB 11505:sv2aHO4S10L002 0:75.52 1:0 A:178830542;C:170284720;G:168594514;T:199773382;N:136435 75 0     178830542 170284720 168594514 199773382 136435 ERX8620331 ERS10857219 ERA9644804 Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive 1 0.94812   0.12868   0.6645   0.47281   75   B   usable mapping rate illumina nextseq unknown poly_a trueseq bulk unknown unknown   Belgium 2022-04-21 Larval Larval Head Nervous System
10429 10429 ERR9077184 ERX8620331 ERS10857219 ERP135966 PRJEB51339 RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae E-MTAB-11505 Transcriptome Analysis Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21   Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. Sv2aHO4S10 SAMEA13254176 Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254176|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHO4S10|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a / |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHO4S10|sex:not available|strain:AB                 NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae E MTAB 11505:Sv2aHO4S10 s Sv2aHO4S10 s RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. Experimental Factor: genotype:sv2a / RNA-Seq TRANSCRIPTOMIC PolyA SINGLE ILLUMINA NextSeq 500   ERP135966 NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 sv2aHO4S10L003-1.fastq.gz fastq 727263626.0 9629858.0 E MTAB 11505:sv2aHO4S10L003 0:75.52 1:0 A:181201278;C:172619520;G:170780953;T:202550397;N:111478 75 0     181201278 172619520 170780953 202550397 111478 ERX8620331 ERS10857219 ERA9644804 Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive 1 0.94768   0.12827   0.66596   0.47332   76   B   usable mapping rate illumina nextseq unknown poly_a trueseq bulk unknown unknown   Belgium 2022-04-21 Larval Larval Head Nervous System
10430 10430 ERR9077185 ERX8620331 ERS10857219 ERP135966 PRJEB51339 RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae E-MTAB-11505 Transcriptome Analysis Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21   Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. Sv2aHO4S10 SAMEA13254176 Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254176|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHO4S10|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a / |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHO4S10|sex:not available|strain:AB                 NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae E MTAB 11505:Sv2aHO4S10 s Sv2aHO4S10 s RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. Experimental Factor: genotype:sv2a / RNA-Seq TRANSCRIPTOMIC PolyA SINGLE ILLUMINA NextSeq 500   ERP135966 NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 sv2aHO4S10L004-1.fastq.gz fastq 736595278.0 9753337.0 E MTAB 11505:sv2aHO4S10L004 0:75.52 1:0 A:183510872;C:174806534;G:173027794;T:205140640;N:109438 75 0     183510872 174806534 173027794 205140640 109438 ERX8620331 ERS10857219 ERA9644804 Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive 1 0.94925   0.12904   0.66417   0.47297   76   B   usable mapping rate illumina nextseq unknown poly_a trueseq bulk unknown unknown   Belgium 2022-04-21 Larval Larval Head Nervous System
10431 10431 ERR9077178 ERX8620330 ERS10857218 ERP135966 PRJEB51339 RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae E-MTAB-11505 Transcriptome Analysis Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21   Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. Sv2aHO3S6 SAMEA13254175 Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254175|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHO3S6|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a / |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHO3S6|sex:not available|strain:AB                 NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae E MTAB 11505:Sv2aHO3S6 s Sv2aHO3S6 s RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. Experimental Factor: genotype:sv2a / RNA-Seq TRANSCRIPTOMIC PolyA SINGLE ILLUMINA NextSeq 500   ERP135966 NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 sv2aHO3S6L001-1.fastq.gz fastq 710756305.0 9410862.0 E MTAB 11505:sv2aHO3S6L001 0:75.53 1:0 A:175585613;C:170261120;G:166658917;T:198106388;N:144267 75 0     175585613 170261120 166658917 198106388 144267 ERX8620330 ERS10857218 ERA9644804 Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive 1 0.94827   0.14002   0.66785   0.474   75   B   usable mapping rate illumina nextseq unknown poly_a trueseq bulk unknown unknown   Belgium 2022-04-21 Larval Larval Head Nervous System
10432 10432 ERR9077179 ERX8620330 ERS10857218 ERP135966 PRJEB51339 RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae E-MTAB-11505 Transcriptome Analysis Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21   Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. Sv2aHO3S6 SAMEA13254175 Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254175|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHO3S6|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a / |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHO3S6|sex:not available|strain:AB                 NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae E MTAB 11505:Sv2aHO3S6 s Sv2aHO3S6 s RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. Experimental Factor: genotype:sv2a / RNA-Seq TRANSCRIPTOMIC PolyA SINGLE ILLUMINA NextSeq 500   ERP135966 NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 sv2aHO3S6L002-1.fastq.gz fastq 715330324.0 9471415.0 E MTAB 11505:sv2aHO3S6L002 0:75.53 1:0 A:176679775;C:171311031;G:167903237;T:199299483;N:136798 75 0     176679775 171311031 167903237 199299483 136798 ERX8620330 ERS10857218 ERA9644804 Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive 1 0.9469   0.13915   0.67004   0.47654   75   B   usable mapping rate illumina nextseq unknown poly_a trueseq bulk unknown unknown   Belgium 2022-04-21 Larval Larval Head Nervous System
10433 10433 ERR9077180 ERX8620330 ERS10857218 ERP135966 PRJEB51339 RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae E-MTAB-11505 Transcriptome Analysis Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21   Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. Sv2aHO3S6 SAMEA13254175 Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254175|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHO3S6|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a / |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHO3S6|sex:not available|strain:AB                 NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae E MTAB 11505:Sv2aHO3S6 s Sv2aHO3S6 s RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. Experimental Factor: genotype:sv2a / RNA-Seq TRANSCRIPTOMIC PolyA SINGLE ILLUMINA NextSeq 500   ERP135966 NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 sv2aHO3S6L003-1.fastq.gz fastq 722810562.0 9570364.0 E MTAB 11505:sv2aHO3S6L003 0:75.53 1:0 A:178442845;C:173192786;G:169595085;T:201465522;N:114324 75 0     178442845 173192786 169595085 201465522 114324 ERX8620330 ERS10857218 ERA9644804 Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive 1 0.94918   0.13979   0.66902   0.47572   75   B   usable mapping rate illumina nextseq unknown poly_a trueseq bulk unknown unknown   Belgium 2022-04-21 Larval Larval Head Nervous System
10434 10434 ERR9077181 ERX8620330 ERS10857218 ERP135966 PRJEB51339 RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae E-MTAB-11505 Transcriptome Analysis Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21   Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. Sv2aHO3S6 SAMEA13254175 Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254175|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHO3S6|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a / |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHO3S6|sex:not available|strain:AB                 NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae E MTAB 11505:Sv2aHO3S6 s Sv2aHO3S6 s RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. Experimental Factor: genotype:sv2a / RNA-Seq TRANSCRIPTOMIC PolyA SINGLE ILLUMINA NextSeq 500   ERP135966 NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 sv2aHO3S6L004-1.fastq.gz fastq 732990507.0 9705054.0 E MTAB 11505:sv2aHO3S6L004 0:75.53 1:0 A:180951130;C:175567778;G:172037542;T:204323488;N:110569 75 0     180951130 175567778 172037542 204323488 110569 ERX8620330 ERS10857218 ERA9644804 Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive 1 0.94812   0.13893   0.67085   0.47502   75   B   usable mapping rate illumina nextseq unknown poly_a trueseq bulk unknown unknown   Belgium 2022-04-21 Larval Larval Head Nervous System
10435 10435 ERR9077174 ERX8620329 ERS10857217 ERP135966 PRJEB51339 RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae E-MTAB-11505 Transcriptome Analysis Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21   Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. Sv2aHET7S9 SAMEA13254174 Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254174|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHET7S9|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/ |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHET7S9|sex:not available|strain:AB                 NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae E MTAB 11505:Sv2aHET7S9 s Sv2aHET7S9 s RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. Experimental Factor: genotype:sv2a+/ RNA-Seq TRANSCRIPTOMIC PolyA SINGLE ILLUMINA NextSeq 500   ERP135966 NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 sv2aHET7S9L001-1.fastq.gz fastq 773209432.0 10238087.0 E MTAB 11505:sv2aHET7S9L001 0:75.52 1:0 A:191962953;C:184699376;G:180774008;T:215620759;N:152336 75 0     191962953 184699376 180774008 215620759 152336 ERX8620329 ERS10857217 ERA9644804 Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive 1 0.95047   0.12485   0.65705   0.4775   76   B   usable mapping rate illumina nextseq unknown poly_a trueseq bulk unknown unknown   Belgium 2022-04-21 Larval Larval Head Nervous System
10436 10436 ERR9077175 ERX8620329 ERS10857217 ERP135966 PRJEB51339 RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae E-MTAB-11505 Transcriptome Analysis Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21   Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. Sv2aHET7S9 SAMEA13254174 Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254174|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHET7S9|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/ |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHET7S9|sex:not available|strain:AB                 NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae E MTAB 11505:Sv2aHET7S9 s Sv2aHET7S9 s RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. Experimental Factor: genotype:sv2a+/ RNA-Seq TRANSCRIPTOMIC PolyA SINGLE ILLUMINA NextSeq 500   ERP135966 NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 sv2aHET7S9L002-1.fastq.gz fastq 773106152.0 10236677.0 E MTAB 11505:sv2aHET7S9L002 0:75.52 1:0 A:191812833;C:184676419;G:180997547;T:215473732;N:145621 75 0     191812833 184676419 180997547 215473732 145621 ERX8620329 ERS10857217 ERA9644804 Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive 1 0.95016   0.12464   0.65482   0.47419   76   B   usable mapping rate illumina nextseq unknown poly_a trueseq bulk unknown unknown   Belgium 2022-04-21 Larval Larval Head Nervous System
10437 10437 ERR9077176 ERX8620329 ERS10857217 ERP135966 PRJEB51339 RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae E-MTAB-11505 Transcriptome Analysis Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21   Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. Sv2aHET7S9 SAMEA13254174 Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254174|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHET7S9|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/ |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHET7S9|sex:not available|strain:AB                 NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae E MTAB 11505:Sv2aHET7S9 s Sv2aHET7S9 s RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. Experimental Factor: genotype:sv2a+/ RNA-Seq TRANSCRIPTOMIC PolyA SINGLE ILLUMINA NextSeq 500   ERP135966 NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 sv2aHET7S9L003-1.fastq.gz fastq 783061051.0 10368445.0 E MTAB 11505:sv2aHET7S9L003 0:75.52 1:0 A:194289152;C:187108364;G:183177036;T:218370500;N:115999 75 0     194289152 187108364 183177036 218370500 115999 ERX8620329 ERS10857217 ERA9644804 Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive 1 0.95172   0.12488   0.65445   0.48157   76   B   usable mapping rate illumina nextseq unknown poly_a trueseq bulk unknown unknown   Belgium 2022-04-21 Larval Larval Head Nervous System
10438 10438 ERR9077177 ERX8620329 ERS10857217 ERP135966 PRJEB51339 RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae E-MTAB-11505 Transcriptome Analysis Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21   Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. Sv2aHET7S9 SAMEA13254174 Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254174|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHET7S9|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/ |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHET7S9|sex:not available|strain:AB                 NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae E MTAB 11505:Sv2aHET7S9 s Sv2aHET7S9 s RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. Experimental Factor: genotype:sv2a+/ RNA-Seq TRANSCRIPTOMIC PolyA SINGLE ILLUMINA NextSeq 500   ERP135966 NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 sv2aHET7S9L004-1.fastq.gz fastq 790150025.0 10462214.0 E MTAB 11505:sv2aHET7S9L004 0:75.52 1:0 A:196056500;C:188746833;G:184927588;T:220305922;N:113182 75 0     196056500 188746833 184927588 220305922 113182 ERX8620329 ERS10857217 ERA9644804 Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive 1 0.95285   0.12478   0.65551   0.4763   76   B   usable mapping rate illumina nextseq unknown poly_a trueseq bulk unknown unknown   Belgium 2022-04-21 Larval Larval Head Nervous System
10439 10439 ERR9077170 ERX8620328 ERS10857216 ERP135966 PRJEB51339 RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae E-MTAB-11505 Transcriptome Analysis Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21   Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. Sv2aHET4S5 SAMEA13254173 Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254173|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHET4S5|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/ |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHET4S5|sex:not available|strain:AB                 NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae E MTAB 11505:Sv2aHET4S5 s Sv2aHET4S5 s RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. Experimental Factor: genotype:sv2a+/ RNA-Seq TRANSCRIPTOMIC PolyA SINGLE ILLUMINA NextSeq 500   ERP135966 NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 sv2aHET4S5L001-1.fastq.gz fastq 685650097.0 9076481.0 E MTAB 11505:sv2aHET4S5L001 0:75.54 1:0 A:161812875;C:167870520;G:166985310;T:188850530;N:130862 75 0     161812875 167870520 166985310 188850530 130862 ERX8620328 ERS10857216 ERA9644804 Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive 1 0.95733   0.09974   0.65691   0.47392   76   B   usable mapping rate illumina nextseq unknown poly_a trueseq bulk unknown unknown   Belgium 2022-04-21 Larval Larval Head Nervous System
10440 10440 ERR9077171 ERX8620328 ERS10857216 ERP135966 PRJEB51339 RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae E-MTAB-11505 Transcriptome Analysis Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21   Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. Sv2aHET4S5 SAMEA13254173 Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254173|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHET4S5|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/ |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHET4S5|sex:not available|strain:AB                 NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae E MTAB 11505:Sv2aHET4S5 s Sv2aHET4S5 s RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. Experimental Factor: genotype:sv2a+/ RNA-Seq TRANSCRIPTOMIC PolyA SINGLE ILLUMINA NextSeq 500   ERP135966 NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 sv2aHET4S5L002-1.fastq.gz fastq 684402241.0 9059974.0 E MTAB 11505:sv2aHET4S5L002 0:75.54 1:0 A:161493428;C:167520248;G:166882054;T:188383816;N:122695 75 0     161493428 167520248 166882054 188383816 122695 ERX8620328 ERS10857216 ERA9644804 Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive 1 0.95537   0.09915   0.65719   0.48261   76   B   usable mapping rate illumina nextseq unknown poly_a trueseq bulk unknown unknown   Belgium 2022-04-21 Larval Larval Head Nervous System
10441 10441 ERR9077172 ERX8620328 ERS10857216 ERP135966 PRJEB51339 RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae E-MTAB-11505 Transcriptome Analysis Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21   Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. Sv2aHET4S5 SAMEA13254173 Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254173|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHET4S5|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/ |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHET4S5|sex:not available|strain:AB                 NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae E MTAB 11505:Sv2aHET4S5 s Sv2aHET4S5 s RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. Experimental Factor: genotype:sv2a+/ RNA-Seq TRANSCRIPTOMIC PolyA SINGLE ILLUMINA NextSeq 500   ERP135966 NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 sv2aHET4S5L003-1.fastq.gz fastq 694099126.0 9188245.0 E MTAB 11505:sv2aHET4S5L003 0:75.54 1:0 A:163727378;C:169989210;G:169143224;T:191137588;N:101726 75 0     163727378 169989210 169143224 191137588 101726 ERX8620328 ERS10857216 ERA9644804 Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive 1 0.95629   0.09861   0.65705   0.47187   75   B   usable mapping rate illumina nextseq unknown poly_a trueseq bulk unknown unknown   Belgium 2022-04-21 Larval Larval Head Nervous System
10442 10442 ERR9077173 ERX8620328 ERS10857216 ERP135966 PRJEB51339 RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae E-MTAB-11505 Transcriptome Analysis Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21   Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. Sv2aHET4S5 SAMEA13254173 Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254173|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHET4S5|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/ |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHET4S5|sex:not available|strain:AB                 NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae E MTAB 11505:Sv2aHET4S5 s Sv2aHET4S5 s RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. Experimental Factor: genotype:sv2a+/ RNA-Seq TRANSCRIPTOMIC PolyA SINGLE ILLUMINA NextSeq 500   ERP135966 NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 sv2aHET4S5L004-1.fastq.gz fastq 698753836.0 9249820.0 E MTAB 11505:sv2aHET4S5L004 0:75.54 1:0 A:164809473;C:171057633;G:170349692;T:192441413;N:95625 75 0     164809473 171057633 170349692 192441413 95625 ERX8620328 ERS10857216 ERA9644804 Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive 1 0.95613   0.09885   0.65798   0.47227   76   B   usable mapping rate illumina nextseq unknown poly_a trueseq bulk unknown unknown   Belgium 2022-04-21 Larval Larval Head Nervous System
10443 10443 ERR9077166 ERX8620327 ERS10857215 ERP135966 PRJEB51339 RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae E-MTAB-11505 Transcriptome Analysis Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21   Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. Sv2aHET3S4 SAMEA13254172 Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254172|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHET3S4|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/ |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHET3S4|sex:not available|strain:AB                 NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae E MTAB 11505:Sv2aHET3S4 s Sv2aHET3S4 s RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. Experimental Factor: genotype:sv2a+/ RNA-Seq TRANSCRIPTOMIC PolyA SINGLE ILLUMINA NextSeq 500   ERP135966 NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 sv2aHET3S4L001-1.fastq.gz fastq 691298605.0 9151512.0 E MTAB 11505:sv2aHET3S4L001 0:75.54 1:0 A:164743919;C:170752099;G:164763621;T:190912044;N:126922 75 0     164743919 170752099 164763621 190912044 126922 ERX8620327 ERS10857215 ERA9644804 Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive 1 0.95684   0.09604   0.6603   0.47285   76   B   usable mapping rate illumina nextseq unknown poly_a trueseq bulk unknown unknown   Belgium 2022-04-21 Larval Larval Head Nervous System
10444 10444 ERR9077167 ERX8620327 ERS10857215 ERP135966 PRJEB51339 RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae E-MTAB-11505 Transcriptome Analysis Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21   Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. Sv2aHET3S4 SAMEA13254172 Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254172|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHET3S4|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/ |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHET3S4|sex:not available|strain:AB                 NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae E MTAB 11505:Sv2aHET3S4 s Sv2aHET3S4 s RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. Experimental Factor: genotype:sv2a+/ RNA-Seq TRANSCRIPTOMIC PolyA SINGLE ILLUMINA NextSeq 500   ERP135966 NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 sv2aHET3S4L002-1.fastq.gz fastq 694080975.0 9188259.0 E MTAB 11505:sv2aHET3S4L002 0:75.54 1:0 A:165336810;C:171397273;G:165632321;T:191591872;N:122699 75 0     165336810 171397273 165632321 191591872 122699 ERX8620327 ERS10857215 ERA9644804 Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive 1 0.95612   0.09694   0.65993   0.46918   76   B   usable mapping rate illumina nextseq unknown poly_a trueseq bulk unknown unknown   Belgium 2022-04-21 Larval Larval Head Nervous System
10445 10445 ERR9077168 ERX8620327 ERS10857215 ERP135966 PRJEB51339 RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae E-MTAB-11505 Transcriptome Analysis Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21   Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. Sv2aHET3S4 SAMEA13254172 Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254172|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHET3S4|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/ |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHET3S4|sex:not available|strain:AB                 NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae E MTAB 11505:Sv2aHET3S4 s Sv2aHET3S4 s RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. Experimental Factor: genotype:sv2a+/ RNA-Seq TRANSCRIPTOMIC PolyA SINGLE ILLUMINA NextSeq 500   ERP135966 NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 sv2aHET3S4L003-1.fastq.gz fastq 703689889.0 9315387.0 E MTAB 11505:sv2aHET3S4L003 0:75.54 1:0 A:167575230;C:173881977;G:167845574;T:194289403;N:97705 75 0     167575230 173881977 167845574 194289403 97705 ERX8620327 ERS10857215 ERA9644804 Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive 1 0.9577   0.09608   0.66042   0.47022   74   B   usable mapping rate illumina nextseq unknown poly_a trueseq bulk unknown unknown   Belgium 2022-04-21 Larval Larval Head Nervous System
10446 10446 ERR9077169 ERX8620327 ERS10857215 ERP135966 PRJEB51339 RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae E-MTAB-11505 Transcriptome Analysis Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21   Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. Sv2aHET3S4 SAMEA13254172 Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254172|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHET3S4|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/ |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHET3S4|sex:not available|strain:AB                 NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae E MTAB 11505:Sv2aHET3S4 s Sv2aHET3S4 s RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. Experimental Factor: genotype:sv2a+/ RNA-Seq TRANSCRIPTOMIC PolyA SINGLE ILLUMINA NextSeq 500   ERP135966 NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 sv2aHET3S4L004-1.fastq.gz fastq 712116538.0 9426961.0 E MTAB 11505:sv2aHET3S4L004 0:75.54 1:0 A:169598596;C:175885948;G:169866534;T:196669400;N:96060 75 0     169598596 175885948 169866534 196669400 96060 ERX8620327 ERS10857215 ERA9644804 Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive 1 0.95734   0.09716   0.66054   0.47257   75   B   usable mapping rate illumina nextseq unknown poly_a trueseq bulk unknown unknown   Belgium 2022-04-21 Larval Larval Head Nervous System
10447 10447 ERR9077162 ERX8620326 ERS10857214 ERP135966 PRJEB51339 RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae E-MTAB-11505 Transcriptome Analysis Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21   Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. Sv2aHET2S3 SAMEA13254171 Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254171|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHET2S3|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/ |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHET2S3|sex:not available|strain:AB                 NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae E MTAB 11505:Sv2aHET2S3 s Sv2aHET2S3 s RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. Experimental Factor: genotype:sv2a+/ RNA-Seq TRANSCRIPTOMIC PolyA SINGLE ILLUMINA NextSeq 500   ERP135966 NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 sv2aHET2S3L001-1.fastq.gz fastq 733440522.0 9709422.0 E MTAB 11505:sv2aHET2S3L001 0:75.54 1:0 A:173670000;C:181175353;G:175392793;T:203059256;N:143120 75 0     173670000 181175353 175392793 203059256 143120 ERX8620326 ERS10857214 ERA9644804 Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive 1 0.95885   0.08758   0.65393   0.47249   76   B   usable mapping rate illumina nextseq unknown poly_a trueseq bulk unknown unknown   Belgium 2022-04-21 Larval Larval Head Nervous System
10448 10448 ERR9077163 ERX8620326 ERS10857214 ERP135966 PRJEB51339 RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae E-MTAB-11505 Transcriptome Analysis Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21   Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. Sv2aHET2S3 SAMEA13254171 Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254171|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHET2S3|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/ |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHET2S3|sex:not available|strain:AB                 NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae E MTAB 11505:Sv2aHET2S3 s Sv2aHET2S3 s RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. Experimental Factor: genotype:sv2a+/ RNA-Seq TRANSCRIPTOMIC PolyA SINGLE ILLUMINA NextSeq 500   ERP135966 NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 sv2aHET2S3L002-1.fastq.gz fastq 732121929.0 9691967.0 E MTAB 11505:sv2aHET2S3L002 0:75.54 1:0 A:173332091;C:180805738;G:175271736;T:202574727;N:137637 75 0     173332091 180805738 175271736 202574727 137637 ERX8620326 ERS10857214 ERA9644804 Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive 1 0.95775   0.08879   0.6564   0.4718   76   B   usable mapping rate illumina nextseq unknown poly_a trueseq bulk unknown unknown   Belgium 2022-04-21 Larval Larval Head Nervous System
10449 10449 ERR9077164 ERX8620326 ERS10857214 ERP135966 PRJEB51339 RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae E-MTAB-11505 Transcriptome Analysis Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21   Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. Sv2aHET2S3 SAMEA13254171 Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254171|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHET2S3|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/ |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHET2S3|sex:not available|strain:AB                 NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae E MTAB 11505:Sv2aHET2S3 s Sv2aHET2S3 s RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. Experimental Factor: genotype:sv2a+/ RNA-Seq TRANSCRIPTOMIC PolyA SINGLE ILLUMINA NextSeq 500   ERP135966 NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 sv2aHET2S3L003-1.fastq.gz fastq 743488189.0 9842286.0 E MTAB 11505:sv2aHET2S3L003 0:75.54 1:0 A:175979238;C:183726319;G:177933958;T:205735955;N:112719 75 0     175979238 183726319 177933958 205735955 112719 ERX8620326 ERS10857214 ERA9644804 Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive 1 0.9593   0.08716   0.65437   0.46976   75   B   usable mapping rate illumina nextseq unknown poly_a trueseq bulk unknown unknown   Belgium 2022-04-21 Larval Larval Head Nervous System
10450 10450 ERR9077165 ERX8620326 ERS10857214 ERP135966 PRJEB51339 RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae E-MTAB-11505 Transcriptome Analysis Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21   Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. Sv2aHET2S3 SAMEA13254171 Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254171|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHET2S3|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/ |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHET2S3|sex:not available|strain:AB                 NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae E MTAB 11505:Sv2aHET2S3 s Sv2aHET2S3 s RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. Experimental Factor: genotype:sv2a+/ RNA-Seq TRANSCRIPTOMIC PolyA SINGLE ILLUMINA NextSeq 500   ERP135966 NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 sv2aHET2S3L004-1.fastq.gz fastq 747723481.0 9898304.0 E MTAB 11505:sv2aHET2S3L004 0:75.54 1:0 A:176935451;C:184684782;G:179046040;T:206946124;N:111084 75 0     176935451 184684782 179046040 206946124 111084 ERX8620326 ERS10857214 ERA9644804 Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive 1 0.95934   0.08676   0.65533   0.47523   75   B   usable mapping rate illumina nextseq unknown poly_a trueseq bulk unknown unknown   Belgium 2022-04-21 Larval Larval Head Nervous System
11025 11025 ERR9787516 ERX9334792 ERS12088364 ERP137937 PRJEB53153 RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae E-MTAB-11776 Transcriptome Analysis Transcriptome analysis of the heads of 5 dpf epdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12   Protocols: 5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines. Sample 9 SAMEA14460887 Laboratory for Molecular Biodiscovery, KU Leuven ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12|External Id:SAMEA14460887|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven|INSDC first public:2022 09 12T12:19:24Z|INSDC last update:2022 09 12T12:19:24Z|INSDC status:public|Submitter Id:E MTAB 11776:Sample 9|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:tsc2 / |individual:pool of 10 larvae|isolate:not applicable|organism part:head|sample name:E MTAB 11776:Sample 9|scientific name:Danio rerio|sex:non available|strain:AB                 Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae E MTAB 11776:Sample 9 p Sample 9 p RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae 5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines. Experimental Factor: genotype:tsc2 / RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina NovaSeq 6000   ERP137937 Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12 TSCHOM1_1.fastq.gz TSCHOM1_2.fastq.gz fastq fastq 6360351174.0 31486887.0 E MTAB 11776:TSCHOM1 0:101 1:101 A:1750731340;C:1410426576;G:1460021447;T:1739131898;N:39913 101 101     1750731340 1410426576 1460021447 1739131898 39913 ERX9334792 ERS12088364 ERA14756702 Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive 2 0.93517 0.92417 0.15551 0.15199 0.67736 0.6786 0.48763 0.48988 101 101 B B biological fallback assumption illumina novaseq_era unknown poly_a trueseq bulk unknown unknown   Belgium 2022-09-12 Larval Larval Head Nervous System
11026 11026 ERR9787515 ERX9334791 ERS12088363 ERP137937 PRJEB53153 RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae E-MTAB-11776 Transcriptome Analysis Transcriptome analysis of the heads of 5 dpf epdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12   Protocols: 5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines. Sample 8 SAMEA14460886 Laboratory for Molecular Biodiscovery, KU Leuven ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12|External Id:SAMEA14460886|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven|INSDC first public:2022 09 12T12:19:24Z|INSDC last update:2022 09 12T12:19:24Z|INSDC status:public|Submitter Id:E MTAB 11776:Sample 8|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:depdc5 / |individual:pool of 10 larvae|isolate:not applicable|organism part:head|sample name:E MTAB 11776:Sample 8|scientific name:Danio rerio|sex:non available|strain:AB                 Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae E MTAB 11776:Sample 8 p Sample 8 p RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae 5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines. Experimental Factor: genotype:depdc5 / RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina NovaSeq 6000   ERP137937 Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12 DEPHOM4_1.fastq.gz DEPHOM4_2.fastq.gz fastq fastq 6195443828.0 30670514.0 E MTAB 11776:DEPHOM4 0:101 1:101 A:1524344281;C:1573560980;G:1585093004;T:1512406843;N:38720 101 101     1524344281 1573560980 1585093004 1512406843 38720 ERX9334791 ERS12088363 ERA14756702 Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive 2 0.95088 0.9542 0.17631 0.17203 0.71121 0.70981 0.56015 0.57999 101 101 B B biological fallback assumption illumina novaseq_era unknown poly_a trueseq bulk unknown unknown   Belgium 2022-09-12 Larval Larval Head Nervous System
11027 11027 ERR9787514 ERX9334790 ERS12088362 ERP137937 PRJEB53153 RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae E-MTAB-11776 Transcriptome Analysis Transcriptome analysis of the heads of 5 dpf epdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12   Protocols: 5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines. Sample 7 SAMEA14460885 Laboratory for Molecular Biodiscovery, KU Leuven ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12|External Id:SAMEA14460885|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven|INSDC first public:2022 09 12T12:19:23Z|INSDC last update:2022 09 12T12:19:23Z|INSDC status:public|Submitter Id:E MTAB 11776:Sample 7|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:depdc5 / |individual:pool of 10 larvae|isolate:not applicable|organism part:head|sample name:E MTAB 11776:Sample 7|scientific name:Danio rerio|sex:non available|strain:AB                 Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae E MTAB 11776:Sample 7 p Sample 7 p RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae 5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines. Experimental Factor: genotype:depdc5 / RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina NovaSeq 6000   ERP137937 Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12 DEPHOM7_1.fastq.gz DEPHOM7_2.fastq.gz fastq fastq 6201660378.0 30701289.0 E MTAB 11776:DEPHOM7 0:101 1:101 A:1699906420;C:1382805098;G:1427335171;T:1691574092;N:39597 101 101     1699906420 1382805098 1427335171 1691574092 39597 ERX9334790 ERS12088362 ERA14756702 Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive 2 0.92781 0.92603 0.15556 0.15416 0.67965 0.67963 0.48209 0.48229 101 101 B B biological fallback assumption illumina novaseq_era unknown poly_a trueseq bulk unknown unknown   Belgium 2022-09-12 Larval Larval Head Nervous System
11028 11028 ERR9787513 ERX9334789 ERS12088361 ERP137937 PRJEB53153 RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae E-MTAB-11776 Transcriptome Analysis Transcriptome analysis of the heads of 5 dpf epdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12   Protocols: 5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines. Sample 6 SAMEA14460884 Laboratory for Molecular Biodiscovery, KU Leuven ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12|External Id:SAMEA14460884|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven|INSDC first public:2022 09 12T12:19:23Z|INSDC last update:2022 09 12T12:19:23Z|INSDC status:public|Submitter Id:E MTAB 11776:Sample 6|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:depdc5 / |individual:pool of 10 larvae|isolate:not applicable|organism part:head|sample name:E MTAB 11776:Sample 6|scientific name:Danio rerio|sex:non available|strain:AB                 Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae E MTAB 11776:Sample 6 p Sample 6 p RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae 5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines. Experimental Factor: genotype:depdc5 / RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina NovaSeq 6000   ERP137937 Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12 DEPHOM6_1.fastq.gz DEPHOM6_2.fastq.gz fastq fastq 6255857382.0 30969591.0 E MTAB 11776:DEPHOM6 0:101 1:101 A:1722554517;C:1398134491;G:1424183345;T:1710945949;N:39080 101 101     1722554517 1398134491 1424183345 1710945949 39080 ERX9334789 ERS12088361 ERA14756702 Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive 2 0.93476 0.93206 0.16121 0.1592 0.67882 0.67898 0.48043 0.47851 101 101 B B biological fallback assumption illumina novaseq_era unknown poly_a trueseq bulk unknown unknown   Belgium 2022-09-12 Larval Larval Head Nervous System
11029 11029 ERR9787512 ERX9334788 ERS12088360 ERP137937 PRJEB53153 RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae E-MTAB-11776 Transcriptome Analysis Transcriptome analysis of the heads of 5 dpf epdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12   Protocols: 5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines. Sample 5 SAMEA14460883 Laboratory for Molecular Biodiscovery, KU Leuven ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12|External Id:SAMEA14460883|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven|INSDC first public:2022 09 12T12:19:23Z|INSDC last update:2022 09 12T12:19:23Z|INSDC status:public|Submitter Id:E MTAB 11776:Sample 5|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:depdc5 / |individual:pool of 10 larvae|isolate:not applicable|organism part:head|sample name:E MTAB 11776:Sample 5|scientific name:Danio rerio|sex:non available|strain:AB                 Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae E MTAB 11776:Sample 5 p Sample 5 p RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae 5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines. Experimental Factor: genotype:depdc5 / RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina NovaSeq 6000   ERP137937 Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12 DEPHOM1_1.fastq.gz DEPHOM1_2.fastq.gz fastq fastq 6340417006.0 31388203.0 E MTAB 11776:DEPHOM1 0:101 1:101 A:1738622221;C:1420060351;G:1455477148;T:1726216833;N:40453 101 101     1738622221 1420060351 1455477148 1726216833 40453 ERX9334788 ERS12088360 ERA14756702 Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive 2 0.93634 0.93167 0.15036 0.14813 0.67809 0.67813 0.47978 0.47851 101 101 B B biological fallback assumption illumina novaseq_era unknown poly_a trueseq bulk unknown unknown   Belgium 2022-09-12 Larval Larval Head Nervous System
11030 11030 ERR9787511 ERX9334787 ERS12088359 ERP137937 PRJEB53153 RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae E-MTAB-11776 Transcriptome Analysis Transcriptome analysis of the heads of 5 dpf epdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12   Protocols: 5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines. Sample 4 SAMEA14460882 Laboratory for Molecular Biodiscovery, KU Leuven ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12|External Id:SAMEA14460882|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven|INSDC first public:2022 09 12T12:19:23Z|INSDC last update:2022 09 12T12:19:23Z|INSDC status:public|Submitter Id:E MTAB 11776:Sample 4|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:depdc5 / x tsc2 / |individual:pool of 10 larvae|isolate:not applicable|organism part:head|sample name:E MTAB 11776:Sample 4|scientific name:Danio rerio|sex:non available|strain:AB                 Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae E MTAB 11776:Sample 4 p Sample 4 p RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae 5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines. Experimental Factor: genotype:depdc5 / x tsc2 / RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina NovaSeq 6000   ERP137937 Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12 2XHOM7_1.fastq.gz 2XHOM7_2.fastq.gz fastq fastq 6291880446.0 31147923.0 E MTAB 11776:2XHOM7 0:101 1:101 A:1712784902;C:1424143534;G:1452943449;T:1701969086;N:39475 101 101     1712784902 1424143534 1452943449 1701969086 39475 ERX9334787 ERS12088359 ERA14756702 Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive 2 0.93869 0.93511 0.15405 0.15042 0.67377 0.67351 0.50599 0.50683 101 101 B B biological fallback assumption illumina novaseq_era unknown poly_a trueseq bulk unknown unknown   Belgium 2022-09-12 Larval Larval Head Nervous System
11031 11031 ERR9787510 ERX9334786 ERS12088358 ERP137937 PRJEB53153 RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae E-MTAB-11776 Transcriptome Analysis Transcriptome analysis of the heads of 5 dpf epdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12   Protocols: 5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines. Sample 3 SAMEA14460881 Laboratory for Molecular Biodiscovery, KU Leuven ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12|External Id:SAMEA14460881|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven|INSDC first public:2022 09 12T12:19:23Z|INSDC last update:2022 09 12T12:19:23Z|INSDC status:public|Submitter Id:E MTAB 11776:Sample 3|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:depdc5 / x tsc2 / |individual:pool of 10 larvae|isolate:not applicable|organism part:head|sample name:E MTAB 11776:Sample 3|scientific name:Danio rerio|sex:non available|strain:AB                 Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae E MTAB 11776:Sample 3 p Sample 3 p RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae 5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines. Experimental Factor: genotype:depdc5 / x tsc2 / RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina NovaSeq 6000   ERP137937 Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12 2XHOM6_1.fastq.gz 2XHOM6_2.fastq.gz fastq fastq 6214327596.0 30763998.0 E MTAB 11776:2XHOM6 0:101 1:101 A:1705019759;C:1384064144;G:1432455427;T:1692749964;N:38302 101 101     1705019759 1384064144 1432455427 1692749964 38302 ERX9334786 ERS12088358 ERA14756702 Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive 2 0.93591 0.92636 0.14492 0.14145 0.67489 0.67491 0.47753 0.49286 101 101 B B biological fallback assumption illumina novaseq_era unknown poly_a trueseq bulk unknown unknown   Belgium 2022-09-12 Larval Larval Head Nervous System
11032 11032 ERR9787509 ERX9334785 ERS12088357 ERP137937 PRJEB53153 RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae E-MTAB-11776 Transcriptome Analysis Transcriptome analysis of the heads of 5 dpf epdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12   Protocols: 5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines. Sample 2 SAMEA14460880 Laboratory for Molecular Biodiscovery, KU Leuven ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12|External Id:SAMEA14460880|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven|INSDC first public:2022 09 12T12:19:23Z|INSDC last update:2022 09 12T12:19:23Z|INSDC status:public|Submitter Id:E MTAB 11776:Sample 2|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:depdc5 / x tsc2 / |individual:pool of 10 larvae|isolate:not applicable|organism part:head|sample name:E MTAB 11776:Sample 2|scientific name:Danio rerio|sex:non available|strain:AB                 Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae E MTAB 11776:Sample 2 p Sample 2 p RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae 5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines. Experimental Factor: genotype:depdc5 / x tsc2 / RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina NovaSeq 6000   ERP137937 Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12 2XHOM4_1.fastq.gz 2XHOM4_2.fastq.gz fastq fastq 6301691990.0 31196495.0 E MTAB 11776:2XHOM4 0:101 1:101 A:1698118705;C:1442276237;G:1473988715;T:1687268823;N:39510 101 101     1698118705 1442276237 1473988715 1687268823 39510 ERX9334785 ERS12088357 ERA14756702 Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive 2 0.94255 0.93839 0.13232 0.13007 0.67326 0.67294 0.49084 0.49291 101 101 B B biological fallback assumption illumina novaseq_era unknown poly_a trueseq bulk unknown unknown   Belgium 2022-09-12 Larval Larval Head Nervous System
11033 11033 ERR9787508 ERX9334784 ERS12088356 ERP137937 PRJEB53153 RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae E-MTAB-11776 Transcriptome Analysis Transcriptome analysis of the heads of 5 dpf epdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12   Protocols: 5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines. Sample 16 SAMEA14460879 Laboratory for Molecular Biodiscovery, KU Leuven ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12|External Id:SAMEA14460879|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven|INSDC first public:2022 09 12T12:19:23Z|INSDC last update:2022 09 12T12:19:23Z|INSDC status:public|Submitter Id:E MTAB 11776:Sample 16|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:wild type genotype|individual:pool of 10 larvae|isolate:not applicable|organism part:head|sample name:E MTAB 11776:Sample 16|scientific name:Danio rerio|sex:non available|strain:AB                 Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae E MTAB 11776:Sample 16 p Sample 16 p RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae 5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines. Experimental Factor: genotype:wild type genotype RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina NovaSeq 6000   ERP137937 Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12 WT7_1.fastq.gz WT7_2.fastq.gz fastq fastq 6206666746.0 30726073.0 E MTAB 11776:WT7 0:101 1:101 A:1705689174;C:1387290312;G:1420249657;T:1693397929;N:39674 101 101     1705689174 1387290312 1420249657 1693397929 39674 ERX9334784 ERS12088356 ERA14756702 Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive 2 0.93493 0.92964 0.15803 0.1554 0.67405 0.67501 0.47988 0.46768 101 101 B B biological fallback assumption illumina novaseq_era unknown poly_a trueseq bulk unknown unknown   Belgium 2022-09-12 Larval Larval Head Nervous System
11034 11034 ERR9787507 ERX9334783 ERS12088355 ERP137937 PRJEB53153 RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae E-MTAB-11776 Transcriptome Analysis Transcriptome analysis of the heads of 5 dpf epdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12   Protocols: 5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines. Sample 15 SAMEA14460878 Laboratory for Molecular Biodiscovery, KU Leuven ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12|External Id:SAMEA14460878|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven|INSDC first public:2022 09 12T12:19:23Z|INSDC last update:2022 09 12T12:19:23Z|INSDC status:public|Submitter Id:E MTAB 11776:Sample 15|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:wild type genotype|individual:pool of 10 larvae|isolate:not applicable|organism part:head|sample name:E MTAB 11776:Sample 15|scientific name:Danio rerio|sex:non available|strain:AB                 Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae E MTAB 11776:Sample 15 p Sample 15 p RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae 5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines. Experimental Factor: genotype:wild type genotype RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina NovaSeq 6000   ERP137937 Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12 WT6_1.fastq.gz WT6_2.fastq.gz fastq fastq 6360740630.0 31488815.0 E MTAB 11776:WT6 0:101 1:101 A:1735195643;C:1436805269;G:1469558083;T:1719141699;N:39936 101 101     1735195643 1436805269 1469558083 1719141699 39936 ERX9334783 ERS12088355 ERA14756702 Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive 2 0.93629 0.93343 0.15169 0.1501 0.67572 0.67517 0.47499 0.47879 101 101 B B biological fallback assumption illumina novaseq_era unknown poly_a trueseq bulk unknown unknown   Belgium 2022-09-12 Larval Larval Head Nervous System
11035 11035 ERR9787506 ERX9334782 ERS12088354 ERP137937 PRJEB53153 RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae E-MTAB-11776 Transcriptome Analysis Transcriptome analysis of the heads of 5 dpf epdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12   Protocols: 5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines. Sample 14 SAMEA14460877 Laboratory for Molecular Biodiscovery, KU Leuven ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12|External Id:SAMEA14460877|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven|INSDC first public:2022 09 12T12:19:23Z|INSDC last update:2022 09 12T12:19:23Z|INSDC status:public|Submitter Id:E MTAB 11776:Sample 14|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:wild type genotype|individual:pool of 10 larvae|isolate:not applicable|organism part:head|sample name:E MTAB 11776:Sample 14|scientific name:Danio rerio|sex:non available|strain:AB                 Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae E MTAB 11776:Sample 14 p Sample 14 p RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae 5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines. Experimental Factor: genotype:wild type genotype RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina NovaSeq 6000   ERP137937 Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12 WT4_1.fastq.gz WT4_2.fastq.gz fastq fastq 6227472342.0 30829071.0 E MTAB 11776:WT4 0:101 1:101 A:1668352581;C:1434152947;G:1468645621;T:1656281986;N:39207 101 101     1668352581 1434152947 1468645621 1656281986 39207 ERX9334782 ERS12088354 ERA14756702 Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive 2 0.94261 0.93675 0.15334 0.15049 0.67927 0.67872 0.48762 0.49138 101 101 B B biological fallback assumption illumina novaseq_era unknown poly_a trueseq bulk unknown unknown   Belgium 2022-09-12 Larval Larval Head Nervous System
11036 11036 ERR9787505 ERX9334781 ERS12088353 ERP137937 PRJEB53153 RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae E-MTAB-11776 Transcriptome Analysis Transcriptome analysis of the heads of 5 dpf epdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12   Protocols: 5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines. Sample 13 SAMEA14460876 Laboratory for Molecular Biodiscovery, KU Leuven ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12|External Id:SAMEA14460876|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven|INSDC first public:2022 09 12T12:19:23Z|INSDC last update:2022 09 12T12:19:23Z|INSDC status:public|Submitter Id:E MTAB 11776:Sample 13|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:wild type genotype|individual:pool of 10 larvae|isolate:not applicable|organism part:head|sample name:E MTAB 11776:Sample 13|scientific name:Danio rerio|sex:non available|strain:AB                 Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae E MTAB 11776:Sample 13 p Sample 13 p RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae 5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines. Experimental Factor: genotype:wild type genotype RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina NovaSeq 6000   ERP137937 Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12 WT1_1.fastq.gz WT1_2.fastq.gz fastq fastq 6332757570.0 31350285.0 E MTAB 11776:WT1 0:101 1:101 A:1726838066;C:1431452165;G:1460746594;T:1713681043;N:39702 101 101     1726838066 1431452165 1460746594 1713681043 39702 ERX9334781 ERS12088353 ERA14756702 Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive 2 0.92422 0.93556 0.14884 0.14926 0.67694 0.67564 0.48223 0.48884 101 101 B B biological fallback assumption illumina novaseq_era unknown poly_a trueseq bulk unknown unknown   Belgium 2022-09-12 Larval Larval Head Nervous System
11037 11037 ERR9787504 ERX9334780 ERS12088352 ERP137937 PRJEB53153 RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae E-MTAB-11776 Transcriptome Analysis Transcriptome analysis of the heads of 5 dpf epdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12   Protocols: 5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines. Sample 12 SAMEA14460875 Laboratory for Molecular Biodiscovery, KU Leuven ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12|External Id:SAMEA14460875|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven|INSDC first public:2022 09 12T12:19:23Z|INSDC last update:2022 09 12T12:19:23Z|INSDC status:public|Submitter Id:E MTAB 11776:Sample 12|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:tsc2 / |individual:pool of 10 larvae|isolate:not applicable|organism part:head|sample name:E MTAB 11776:Sample 12|scientific name:Danio rerio|sex:non available|strain:AB                 Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae E MTAB 11776:Sample 12 p Sample 12 p RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae 5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines. Experimental Factor: genotype:tsc2 / RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina NovaSeq 6000   ERP137937 Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12 TSCHOM7_1.fastq.gz TSCHOM7_2.fastq.gz fastq fastq 6216990360.0 30777180.0 E MTAB 11776:TSCHOM7 0:101 1:101 A:1710256020;C:1390027205;G:1419250792;T:1697417099;N:39244 101 101     1710256020 1390027205 1419250792 1697417099 39244 ERX9334780 ERS12088352 ERA14756702 Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive 2 0.93162 0.92801 0.15191 0.14943 0.67789 0.67951 0.47915 0.47762 101 101 B B biological fallback assumption illumina novaseq_era unknown poly_a trueseq bulk unknown unknown   Belgium 2022-09-12 Larval Larval Head Nervous System
11038 11038 ERR9787503 ERX9334779 ERS12088351 ERP137937 PRJEB53153 RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae E-MTAB-11776 Transcriptome Analysis Transcriptome analysis of the heads of 5 dpf epdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12   Protocols: 5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines. Sample 11 SAMEA14460874 Laboratory for Molecular Biodiscovery, KU Leuven ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12|External Id:SAMEA14460874|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven|INSDC first public:2022 09 12T12:19:23Z|INSDC last update:2022 09 12T12:19:23Z|INSDC status:public|Submitter Id:E MTAB 11776:Sample 11|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:tsc2 / |individual:pool of 10 larvae|isolate:not applicable|organism part:head|sample name:E MTAB 11776:Sample 11|scientific name:Danio rerio|sex:non available|strain:AB                 Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae E MTAB 11776:Sample 11 p Sample 11 p RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae 5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines. Experimental Factor: genotype:tsc2 / RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina NovaSeq 6000   ERP137937 Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12 TSCHOM6_1.fastq.gz TSCHOM6_2.fastq.gz fastq fastq 6199081848.0 30688524.0 E MTAB 11776:TSCHOM6 0:101 1:101 A:1705859045;C:1387530558;G:1415224718;T:1690428701;N:38826 101 101     1705859045 1387530558 1415224718 1690428701 38826 ERX9334779 ERS12088351 ERA14756702 Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive 2 0.93583 0.93349 0.15923 0.15664 0.67976 0.67986 0.48632 0.48376 101 101 B B biological fallback assumption illumina novaseq_era unknown poly_a trueseq bulk unknown unknown   Belgium 2022-09-12 Larval Larval Head Nervous System
11039 11039 ERR9787502 ERX9334778 ERS12088350 ERP137937 PRJEB53153 RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae E-MTAB-11776 Transcriptome Analysis Transcriptome analysis of the heads of 5 dpf epdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12   Protocols: 5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines. Sample 10 SAMEA14460873 Laboratory for Molecular Biodiscovery, KU Leuven ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12|External Id:SAMEA14460873|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven|INSDC first public:2022 09 12T12:19:23Z|INSDC last update:2022 09 12T12:19:23Z|INSDC status:public|Submitter Id:E MTAB 11776:Sample 10|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:tsc2 / |individual:pool of 10 larvae|isolate:not applicable|organism part:head|sample name:E MTAB 11776:Sample 10|scientific name:Danio rerio|sex:non available|strain:AB                 Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae E MTAB 11776:Sample 10 p Sample 10 p RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae 5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines. Experimental Factor: genotype:tsc2 / RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina NovaSeq 6000   ERP137937 Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12 TSCHOM4_1.fastq.gz TSCHOM4_2.fastq.gz fastq fastq 6287836204.0 31127902.0 E MTAB 11776:TSCHOM4 0:101 1:101 A:1673762523;C:1453880149;G:1493343671;T:1666809849;N:40012 101 101     1673762523 1453880149 1493343671 1666809849 40012 ERX9334778 ERS12088350 ERA14756702 Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive 2 0.94269 0.9375 0.14166 0.13979 0.67476 0.67722 0.4927 0.49365 101 101 B B biological fallback assumption illumina novaseq_era unknown poly_a trueseq bulk unknown unknown   Belgium 2022-09-12 Larval Larval Head Nervous System
11040 11040 ERR9787501 ERX9334777 ERS12088349 ERP137937 PRJEB53153 RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae E-MTAB-11776 Transcriptome Analysis Transcriptome analysis of the heads of 5 dpf epdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12   Protocols: 5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines. Sample 1 SAMEA14460872 Laboratory for Molecular Biodiscovery, KU Leuven ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12|External Id:SAMEA14460872|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven|INSDC first public:2022 09 12T12:19:23Z|INSDC last update:2022 09 12T12:19:23Z|INSDC status:public|Submitter Id:E MTAB 11776:Sample 1|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:depdc5 / x tsc2 / |individual:pool of 10 larvae|isolate:not applicable|organism part:head|sample name:E MTAB 11776:Sample 1|scientific name:Danio rerio|sex:non available|strain:AB                 Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae E MTAB 11776:Sample 1 p Sample 1 p RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae 5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines. Experimental Factor: genotype:depdc5 / x tsc2 / RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina NovaSeq 6000   ERP137937 Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 / tsc2 / depdc5 / x tsc2 / and wildtype zebrafish larvae ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12 2XHOM1_1.fastq.gz 2XHOM1_2.fastq.gz fastq fastq 6232605768.0 30854484.0 E MTAB 11776:2XHOM1 0:101 1:101 A:1725401812;C:1384507160;G:1410306583;T:1712351429;N:38784 101 101     1725401812 1384507160 1410306583 1712351429 38784 ERX9334777 ERS12088349 ERA14756702 Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive 2 0.93594 0.93262 0.15282 0.14977 0.67503 0.67373 0.49522 0.49759 101 101 B B biological fallback assumption illumina novaseq_era unknown poly_a trueseq bulk unknown unknown   Belgium 2022-09-12 Larval Larval Head Nervous System
11711 11711 ERR11282347 ERX10689990 ERS15395535 ERP146895 PRJEB61813 RNA seq of brain tissues of WT and abcb4 knockout zebrafish at 2 month and 30 month E-MTAB-12901 Other The ATP binding cassette subfamily B member 1 ABCB1 encoding a multidrug transporter P glycoprotein plays a critical role in the efflux of xenobiotics in humans and is implicated in cancer resistance to chemotherapy—however little information regarding Pgp at the zebrafish. In addition to study the function of Pgp in the zebrafish brain in the aging process we performed RNA seq using brain tissue of WT and abcb4 knockout zebrafish at different ages such as 2 month and 30 month. ENA FIRST PUBLIC:2023 07 14|ENA LAST UPDATE:2023 07 14   Protocols: brain tissue were dissected from zebrafish and snap frozen until RNA isolation Trizol + zymo RNA clean column kit TruSeq RNA sample prep kit Illumina to prepare cDNA libraries post PolyA selection Sample 8 SAMEA113400389 VANDERBILT UNIVERSITY ENA FIRST PUBLIC:2023 07 14T00:23:41Z|ENA LAST UPDATE:2023 07 14T00:23:41Z|External Id:SAMEA113400389|INSDC center alias:VANDERBILT UNIVERSITY|INSDC center name:VANDERBILT UNIVERSITY|INSDC first public:2023 07 14T00:23:41Z|INSDC last update:2023 07 14T00:23:41Z|INSDC status:public|Submitter Id:E MTAB 12901:Sample 8|age:2|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|disease:normal|genotype:wild type genotype|organism part:brain|sample name:E MTAB 12901:Sample 8|scientific name:Danio rerio|sex:female|strain:AB                 Illumina NovaSeq 6000 paired end sequencing; RNA seq of brain tissues of WT and abcb4 knockout zebrafish at 2 month and 30 month E MTAB 12901:Sample 8 p Sample 8 p RNA seq of brain tissues of WT and abcb4 knockout zebrafish at 2 month and 30 month brain tissue were dissected from zebrafish and snap frozen until RNA isolation Trizol + zymo RNA clean column kit TruSeq RNA sample prep kit Illumina to prepare cDNA libraries post PolyA selection Experimental Factor: genotype:wild type genotype RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina NovaSeq 6000   ERP146895 Illumina NovaSeq 6000 paired end sequencing; RNA seq of brain tissues of WT and abcb4 knockout zebrafish at 2 month and 30 month ENA FIRST PUBLIC:2023 07 14|ENA LAST UPDATE:2023 07 14 8680-JP-0008_S1_L005_R2_001.fastq.gz 8680-JP-0008_S1_L005_R1_001.fastq.gz fastq fastq 21919844332.0 72582266.0 E MTAB 12901:8680 JP 0008 S1 L005 R 0:151 1:151 A:5996325335;C:4770833694;G:5244802952;T:5876120950;N:31761401 151 151     5996325335 4770833694 5244802952 5876120950 31761401 ERX10689990 ERS15395535 ERA23235100 VANDERBILT UNIVERSITY|European Nucleotide Archive VANDERBILT UNIVERSITY|European Nucleotide Archive 2 0.92572 0.92425 0.13427 0.13101 0.70086 0.70266 0.50868 0.50381 151 151 B B biological fallback assumption illumina novaseq_era unknown poly_a trueseq bulk unknown unknown   United States 2023-07-14 Adult Adult Brain Nervous System
11712 11712 ERR11282346 ERX10689989 ERS15395534 ERP146895 PRJEB61813 RNA seq of brain tissues of WT and abcb4 knockout zebrafish at 2 month and 30 month E-MTAB-12901 Other The ATP binding cassette subfamily B member 1 ABCB1 encoding a multidrug transporter P glycoprotein plays a critical role in the efflux of xenobiotics in humans and is implicated in cancer resistance to chemotherapy—however little information regarding Pgp at the zebrafish. In addition to study the function of Pgp in the zebrafish brain in the aging process we performed RNA seq using brain tissue of WT and abcb4 knockout zebrafish at different ages such as 2 month and 30 month. ENA FIRST PUBLIC:2023 07 14|ENA LAST UPDATE:2023 07 14   Protocols: brain tissue were dissected from zebrafish and snap frozen until RNA isolation Trizol + zymo RNA clean column kit TruSeq RNA sample prep kit Illumina to prepare cDNA libraries post PolyA selection Sample 7 SAMEA113400388 VANDERBILT UNIVERSITY ENA FIRST PUBLIC:2023 07 14T00:23:41Z|ENA LAST UPDATE:2023 07 14T00:23:41Z|External Id:SAMEA113400388|INSDC center alias:VANDERBILT UNIVERSITY|INSDC center name:VANDERBILT UNIVERSITY|INSDC first public:2023 07 14T00:23:41Z|INSDC last update:2023 07 14T00:23:41Z|INSDC status:public|Submitter Id:E MTAB 12901:Sample 7|age:2|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|disease:normal|genotype:wild type genotype|organism part:brain|sample name:E MTAB 12901:Sample 7|scientific name:Danio rerio|sex:female|strain:AB                 Illumina NovaSeq 6000 paired end sequencing; RNA seq of brain tissues of WT and abcb4 knockout zebrafish at 2 month and 30 month E MTAB 12901:Sample 7 p Sample 7 p RNA seq of brain tissues of WT and abcb4 knockout zebrafish at 2 month and 30 month brain tissue were dissected from zebrafish and snap frozen until RNA isolation Trizol + zymo RNA clean column kit TruSeq RNA sample prep kit Illumina to prepare cDNA libraries post PolyA selection Experimental Factor: genotype:wild type genotype RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina NovaSeq 6000   ERP146895 Illumina NovaSeq 6000 paired end sequencing; RNA seq of brain tissues of WT and abcb4 knockout zebrafish at 2 month and 30 month ENA FIRST PUBLIC:2023 07 14|ENA LAST UPDATE:2023 07 14 8680-JP-0007_S1_L005_R2_001.fastq.gz 8680-JP-0007_S1_L005_R1_001.fastq.gz fastq fastq 20351465316.0 67388958.0 E MTAB 12901:8680 JP 0007 S1 L005 R 0:151 1:151 A:5563989836;C:4461170993;G:4892290448;T:5404916249;N:29097790 151 151     5563989836 4461170993 4892290448 5404916249 29097790 ERX10689989 ERS15395534 ERA23235100 VANDERBILT UNIVERSITY|European Nucleotide Archive VANDERBILT UNIVERSITY|European Nucleotide Archive 2 0.9267 0.9236 0.12782 0.12528 0.70307 0.70473 0.50799 0.50969 151 151 B B biological fallback assumption illumina novaseq_era unknown poly_a trueseq bulk unknown unknown   United States 2023-07-14 Adult Adult Brain Nervous System
11713 11713 ERR11282345 ERX10689988 ERS15395533 ERP146895 PRJEB61813 RNA seq of brain tissues of WT and abcb4 knockout zebrafish at 2 month and 30 month E-MTAB-12901 Other The ATP binding cassette subfamily B member 1 ABCB1 encoding a multidrug transporter P glycoprotein plays a critical role in the efflux of xenobiotics in humans and is implicated in cancer resistance to chemotherapy—however little information regarding Pgp at the zebrafish. In addition to study the function of Pgp in the zebrafish brain in the aging process we performed RNA seq using brain tissue of WT and abcb4 knockout zebrafish at different ages such as 2 month and 30 month. ENA FIRST PUBLIC:2023 07 14|ENA LAST UPDATE:2023 07 14   Protocols: brain tissue were dissected from zebrafish and snap frozen until RNA isolation Trizol + zymo RNA clean column kit TruSeq RNA sample prep kit Illumina to prepare cDNA libraries post PolyA selection Sample 6 SAMEA113400387 VANDERBILT UNIVERSITY ENA FIRST PUBLIC:2023 07 14T00:23:41Z|ENA LAST UPDATE:2023 07 14T00:23:41Z|External Id:SAMEA113400387|INSDC center alias:VANDERBILT UNIVERSITY|INSDC center name:VANDERBILT UNIVERSITY|INSDC first public:2023 07 14T00:23:41Z|INSDC last update:2023 07 14T00:23:41Z|INSDC status:public|Submitter Id:E MTAB 12901:Sample 6|age:2|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|disease:normal|genotype:wild type genotype|organism part:brain|sample name:E MTAB 12901:Sample 6|scientific name:Danio rerio|sex:female|strain:AB                 Illumina NovaSeq 6000 paired end sequencing; RNA seq of brain tissues of WT and abcb4 knockout zebrafish at 2 month and 30 month E MTAB 12901:Sample 6 p Sample 6 p RNA seq of brain tissues of WT and abcb4 knockout zebrafish at 2 month and 30 month brain tissue were dissected from zebrafish and snap frozen until RNA isolation Trizol + zymo RNA clean column kit TruSeq RNA sample prep kit Illumina to prepare cDNA libraries post PolyA selection Experimental Factor: genotype:wild type genotype RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina NovaSeq 6000   ERP146895 Illumina NovaSeq 6000 paired end sequencing; RNA seq of brain tissues of WT and abcb4 knockout zebrafish at 2 month and 30 month ENA FIRST PUBLIC:2023 07 14|ENA LAST UPDATE:2023 07 14 8680-JP-0006_S1_L005_R2_001.fastq.gz 8680-JP-0006_S1_L005_R1_001.fastq.gz fastq fastq 19220102648.0 63642724.0 E MTAB 12901:8680 JP 0006 S1 L005 R 0:151 1:151 A:5250476003;C:4214339559;G:4606611173;T:5120701154;N:27974759 151 151     5250476003 4214339559 4606611173 5120701154 27974759 ERX10689988 ERS15395533 ERA23235100 VANDERBILT UNIVERSITY|European Nucleotide Archive VANDERBILT UNIVERSITY|European Nucleotide Archive 2 0.92441 0.92391 0.13718 0.13346 0.70281 0.7024 0.48155 0.49173 151 151 B B biological fallback assumption illumina novaseq_era unknown poly_a trueseq bulk unknown unknown   United States 2023-07-14 Adult Adult Brain Nervous System
11714 11714 ERR11282344 ERX10689987 ERS15395532 ERP146895 PRJEB61813 RNA seq of brain tissues of WT and abcb4 knockout zebrafish at 2 month and 30 month E-MTAB-12901 Other The ATP binding cassette subfamily B member 1 ABCB1 encoding a multidrug transporter P glycoprotein plays a critical role in the efflux of xenobiotics in humans and is implicated in cancer resistance to chemotherapy—however little information regarding Pgp at the zebrafish. In addition to study the function of Pgp in the zebrafish brain in the aging process we performed RNA seq using brain tissue of WT and abcb4 knockout zebrafish at different ages such as 2 month and 30 month. ENA FIRST PUBLIC:2023 07 14|ENA LAST UPDATE:2023 07 14   Protocols: brain tissue were dissected from zebrafish and snap frozen until RNA isolation Trizol + zymo RNA clean column kit TruSeq RNA sample prep kit Illumina to prepare cDNA libraries post PolyA selection Sample 3 SAMEA113400386 VANDERBILT UNIVERSITY ENA FIRST PUBLIC:2023 07 14T00:23:41Z|ENA LAST UPDATE:2023 07 14T00:23:41Z|External Id:SAMEA113400386|INSDC center alias:VANDERBILT UNIVERSITY|INSDC center name:VANDERBILT UNIVERSITY|INSDC first public:2023 07 14T00:23:41Z|INSDC last update:2023 07 14T00:23:41Z|INSDC status:public|Submitter Id:E MTAB 12901:Sample 3|age:30|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|disease:normal|genotype:wild type genotype|organism part:brain|sample name:E MTAB 12901:Sample 3|scientific name:Danio rerio|sex:female|strain:AB                 Illumina NovaSeq 6000 paired end sequencing; RNA seq of brain tissues of WT and abcb4 knockout zebrafish at 2 month and 30 month E MTAB 12901:Sample 3 p Sample 3 p RNA seq of brain tissues of WT and abcb4 knockout zebrafish at 2 month and 30 month brain tissue were dissected from zebrafish and snap frozen until RNA isolation Trizol + zymo RNA clean column kit TruSeq RNA sample prep kit Illumina to prepare cDNA libraries post PolyA selection Experimental Factor: genotype:wild type genotype RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina NovaSeq 6000   ERP146895 Illumina NovaSeq 6000 paired end sequencing; RNA seq of brain tissues of WT and abcb4 knockout zebrafish at 2 month and 30 month ENA FIRST PUBLIC:2023 07 14|ENA LAST UPDATE:2023 07 14 8680-JP-0003_S1_L005_R1_001.fastq.gz 8680-JP-0003_S1_L005_R2_001.fastq.gz fastq fastq 18624364462.0 61670081.0 E MTAB 12901:8680 JP 0003 S1 L005 R 0:151 1:151 A:5114421711;C:4075705896;G:4436341056;T:4970953410;N:26942389 151 151     5114421711 4075705896 4436341056 4970953410 26942389 ERX10689987 ERS15395532 ERA23235100 VANDERBILT UNIVERSITY|European Nucleotide Archive VANDERBILT UNIVERSITY|European Nucleotide Archive 2 0.92553 0.92378 0.13069 0.12709 0.69988 0.70258 0.4969 0.51157 151 151 B B biological fallback assumption illumina novaseq_era unknown poly_a trueseq bulk unknown unknown   United States 2023-07-14 Adult Adult Brain Nervous System
11715 11715 ERR11282343 ERX10689986 ERS15395531 ERP146895 PRJEB61813 RNA seq of brain tissues of WT and abcb4 knockout zebrafish at 2 month and 30 month E-MTAB-12901 Other The ATP binding cassette subfamily B member 1 ABCB1 encoding a multidrug transporter P glycoprotein plays a critical role in the efflux of xenobiotics in humans and is implicated in cancer resistance to chemotherapy—however little information regarding Pgp at the zebrafish. In addition to study the function of Pgp in the zebrafish brain in the aging process we performed RNA seq using brain tissue of WT and abcb4 knockout zebrafish at different ages such as 2 month and 30 month. ENA FIRST PUBLIC:2023 07 14|ENA LAST UPDATE:2023 07 14   Protocols: brain tissue were dissected from zebrafish and snap frozen until RNA isolation Trizol + zymo RNA clean column kit TruSeq RNA sample prep kit Illumina to prepare cDNA libraries post PolyA selection Sample 2 SAMEA113400385 VANDERBILT UNIVERSITY ENA FIRST PUBLIC:2023 07 14T00:23:41Z|ENA LAST UPDATE:2023 07 14T00:23:41Z|External Id:SAMEA113400385|INSDC center alias:VANDERBILT UNIVERSITY|INSDC center name:VANDERBILT UNIVERSITY|INSDC first public:2023 07 14T00:23:41Z|INSDC last update:2023 07 14T00:23:41Z|INSDC status:public|Submitter Id:E MTAB 12901:Sample 2|age:30|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|disease:normal|genotype:wild type genotype|organism part:brain|sample name:E MTAB 12901:Sample 2|scientific name:Danio rerio|sex:female|strain:AB                 Illumina NovaSeq 6000 paired end sequencing; RNA seq of brain tissues of WT and abcb4 knockout zebrafish at 2 month and 30 month E MTAB 12901:Sample 2 p Sample 2 p RNA seq of brain tissues of WT and abcb4 knockout zebrafish at 2 month and 30 month brain tissue were dissected from zebrafish and snap frozen until RNA isolation Trizol + zymo RNA clean column kit TruSeq RNA sample prep kit Illumina to prepare cDNA libraries post PolyA selection Experimental Factor: genotype:wild type genotype RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina NovaSeq 6000   ERP146895 Illumina NovaSeq 6000 paired end sequencing; RNA seq of brain tissues of WT and abcb4 knockout zebrafish at 2 month and 30 month ENA FIRST PUBLIC:2023 07 14|ENA LAST UPDATE:2023 07 14 8680-JP-0002_S1_L005_R1_001.fastq.gz 8680-JP-0002_S1_L005_R2_001.fastq.gz fastq fastq 15153807038.0 50178169.0 E MTAB 12901:8680 JP 0002 S1 L005 R 0:151 1:151 A:4156183785;C:3309476575;G:3635942314;T:4030261947;N:21942417 151 151     4156183785 3309476575 3635942314 4030261947 21942417 ERX10689986 ERS15395531 ERA23235100 VANDERBILT UNIVERSITY|European Nucleotide Archive VANDERBILT UNIVERSITY|European Nucleotide Archive 2 0.92069 0.91964 0.13733 0.13466 0.70102 0.70185 0.48507 0.48992 151 151 B B biological fallback assumption illumina novaseq_era unknown poly_a trueseq bulk unknown unknown   United States 2023-07-14 Adult Adult Brain Nervous System
11716 11716 ERR11282342 ERX10689985 ERS15395530 ERP146895 PRJEB61813 RNA seq of brain tissues of WT and abcb4 knockout zebrafish at 2 month and 30 month E-MTAB-12901 Other The ATP binding cassette subfamily B member 1 ABCB1 encoding a multidrug transporter P glycoprotein plays a critical role in the efflux of xenobiotics in humans and is implicated in cancer resistance to chemotherapy—however little information regarding Pgp at the zebrafish. In addition to study the function of Pgp in the zebrafish brain in the aging process we performed RNA seq using brain tissue of WT and abcb4 knockout zebrafish at different ages such as 2 month and 30 month. ENA FIRST PUBLIC:2023 07 14|ENA LAST UPDATE:2023 07 14   Protocols: brain tissue were dissected from zebrafish and snap frozen until RNA isolation Trizol + zymo RNA clean column kit TruSeq RNA sample prep kit Illumina to prepare cDNA libraries post PolyA selection Sample 1 SAMEA113400384 VANDERBILT UNIVERSITY ENA FIRST PUBLIC:2023 07 14T00:23:41Z|ENA LAST UPDATE:2023 07 14T00:23:41Z|External Id:SAMEA113400384|INSDC center alias:VANDERBILT UNIVERSITY|INSDC center name:VANDERBILT UNIVERSITY|INSDC first public:2023 07 14T00:23:41Z|INSDC last update:2023 07 14T00:23:41Z|INSDC status:public|Submitter Id:E MTAB 12901:Sample 1|age:30|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|disease:normal|genotype:wild type genotype|organism part:brain|sample name:E MTAB 12901:Sample 1|scientific name:Danio rerio|sex:female|strain:AB                 Illumina NovaSeq 6000 paired end sequencing; RNA seq of brain tissues of WT and abcb4 knockout zebrafish at 2 month and 30 month E MTAB 12901:Sample 1 p Sample 1 p RNA seq of brain tissues of WT and abcb4 knockout zebrafish at 2 month and 30 month brain tissue were dissected from zebrafish and snap frozen until RNA isolation Trizol + zymo RNA clean column kit TruSeq RNA sample prep kit Illumina to prepare cDNA libraries post PolyA selection Experimental Factor: genotype:wild type genotype RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina NovaSeq 6000   ERP146895 Illumina NovaSeq 6000 paired end sequencing; RNA seq of brain tissues of WT and abcb4 knockout zebrafish at 2 month and 30 month ENA FIRST PUBLIC:2023 07 14|ENA LAST UPDATE:2023 07 14 8680-JP-0001_S1_L005_R2_001.fastq.gz 8680-JP-0001_S1_L005_R1_001.fastq.gz fastq fastq 17758550696.0 58803148.0 E MTAB 12901:8680 JP 0001 S1 L005 R 0:151 1:151 A:4866917250;C:3906782394;G:4241519777;T:4717566830;N:25764445 151 151     4866917250 3906782394 4241519777 4717566830 25764445 ERX10689985 ERS15395530 ERA23235100 VANDERBILT UNIVERSITY|European Nucleotide Archive VANDERBILT UNIVERSITY|European Nucleotide Archive 2 0.92263 0.92154 0.13341 0.12969 0.69443 0.69658 0.48648 0.49678 151 151 B B biological fallback assumption illumina novaseq_era unknown poly_a trueseq bulk unknown unknown   United States 2023-07-14 Adult Adult Brain Nervous System
11717 11717 ERR11282341 ERX10689984 ERS15395529 ERP146895 PRJEB61813 RNA seq of brain tissues of WT and abcb4 knockout zebrafish at 2 month and 30 month E-MTAB-12901 Other The ATP binding cassette subfamily B member 1 ABCB1 encoding a multidrug transporter P glycoprotein plays a critical role in the efflux of xenobiotics in humans and is implicated in cancer resistance to chemotherapy—however little information regarding Pgp at the zebrafish. In addition to study the function of Pgp in the zebrafish brain in the aging process we performed RNA seq using brain tissue of WT and abcb4 knockout zebrafish at different ages such as 2 month and 30 month. ENA FIRST PUBLIC:2023 07 14|ENA LAST UPDATE:2023 07 14   Protocols: brain tissue were dissected from zebrafish and snap frozen until RNA isolation Trizol + zymo RNA clean column kit TruSeq RNA sample prep kit Illumina to prepare cDNA libraries post PolyA selection Sample 9 SAMEA113400383 VANDERBILT UNIVERSITY ENA FIRST PUBLIC:2023 07 14T00:23:41Z|ENA LAST UPDATE:2023 07 14T00:23:41Z|External Id:SAMEA113400383|INSDC center alias:VANDERBILT UNIVERSITY|INSDC center name:VANDERBILT UNIVERSITY|INSDC first public:2023 07 14T00:23:41Z|INSDC last update:2023 07 14T00:23:41Z|INSDC status:public|Submitter Id:E MTAB 12901:Sample 9|age:2|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|disease:normal|genotype:abcb4 / |organism part:brain|sample name:E MTAB 12901:Sample 9|scientific name:Danio rerio|sex:female|strain:AB                 Illumina NovaSeq 6000 paired end sequencing; RNA seq of brain tissues of WT and abcb4 knockout zebrafish at 2 month and 30 month E MTAB 12901:Sample 9 p Sample 9 p RNA seq of brain tissues of WT and abcb4 knockout zebrafish at 2 month and 30 month brain tissue were dissected from zebrafish and snap frozen until RNA isolation Trizol + zymo RNA clean column kit TruSeq RNA sample prep kit Illumina to prepare cDNA libraries post PolyA selection Experimental Factor: genotype:abcb4 / RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina NovaSeq 6000   ERP146895 Illumina NovaSeq 6000 paired end sequencing; RNA seq of brain tissues of WT and abcb4 knockout zebrafish at 2 month and 30 month ENA FIRST PUBLIC:2023 07 14|ENA LAST UPDATE:2023 07 14 8680-JP-0009_S1_L005_R2_001.fastq.gz 8680-JP-0009_S1_L005_R1_001.fastq.gz fastq fastq 21619735456.0 71588528.0 E MTAB 12901:8680 JP 0009 S1 L005 R 0:151 1:151 A:5904040180;C:4720941204;G:5229712921;T:5733559571;N:31481580 151 151     5904040180 4720941204 5229712921 5733559571 31481580 ERX10689984 ERS15395529 ERA23235100 VANDERBILT UNIVERSITY|European Nucleotide Archive VANDERBILT UNIVERSITY|European Nucleotide Archive 2 0.92235 0.92126 0.13956 0.13682 0.70378 0.70526 0.4929 0.49286 151 151 B B biological fallback assumption illumina novaseq_era unknown poly_a trueseq bulk unknown unknown   United States 2023-07-14 Adult Adult Brain Nervous System
11718 11718 ERR11282340 ERX10689983 ERS15395528 ERP146895 PRJEB61813 RNA seq of brain tissues of WT and abcb4 knockout zebrafish at 2 month and 30 month E-MTAB-12901 Other The ATP binding cassette subfamily B member 1 ABCB1 encoding a multidrug transporter P glycoprotein plays a critical role in the efflux of xenobiotics in humans and is implicated in cancer resistance to chemotherapy—however little information regarding Pgp at the zebrafish. In addition to study the function of Pgp in the zebrafish brain in the aging process we performed RNA seq using brain tissue of WT and abcb4 knockout zebrafish at different ages such as 2 month and 30 month. ENA FIRST PUBLIC:2023 07 14|ENA LAST UPDATE:2023 07 14   Protocols: brain tissue were dissected from zebrafish and snap frozen until RNA isolation Trizol + zymo RNA clean column kit TruSeq RNA sample prep kit Illumina to prepare cDNA libraries post PolyA selection Sample 11 SAMEA113400382 VANDERBILT UNIVERSITY ENA FIRST PUBLIC:2023 07 14T00:23:41Z|ENA LAST UPDATE:2023 07 14T00:23:41Z|External Id:SAMEA113400382|INSDC center alias:VANDERBILT UNIVERSITY|INSDC center name:VANDERBILT UNIVERSITY|INSDC first public:2023 07 14T00:23:41Z|INSDC last update:2023 07 14T00:23:41Z|INSDC status:public|Submitter Id:E MTAB 12901:Sample 11|age:2|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|disease:normal|genotype:abcb4 / |organism part:brain|sample name:E MTAB 12901:Sample 11|scientific name:Danio rerio|sex:female|strain:AB                 Illumina NovaSeq 6000 paired end sequencing; RNA seq of brain tissues of WT and abcb4 knockout zebrafish at 2 month and 30 month E MTAB 12901:Sample 11 p Sample 11 p RNA seq of brain tissues of WT and abcb4 knockout zebrafish at 2 month and 30 month brain tissue were dissected from zebrafish and snap frozen until RNA isolation Trizol + zymo RNA clean column kit TruSeq RNA sample prep kit Illumina to prepare cDNA libraries post PolyA selection Experimental Factor: genotype:abcb4 / RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina NovaSeq 6000   ERP146895 Illumina NovaSeq 6000 paired end sequencing; RNA seq of brain tissues of WT and abcb4 knockout zebrafish at 2 month and 30 month ENA FIRST PUBLIC:2023 07 14|ENA LAST UPDATE:2023 07 14 8680-JP-0011_S1_L005_R1_001.fastq.gz 8680-JP-0011_S1_L005_R2_001.fastq.gz fastq fastq 20372991574.0 67460237.0 E MTAB 12901:8680 JP 0011 S1 L005 R 0:151 1:151 A:5569250549;C:4448986210;G:4925237941;T:5399892024;N:29624850 151 151     5569250549 4448986210 4925237941 5399892024 29624850 ERX10689983 ERS15395528 ERA23235100 VANDERBILT UNIVERSITY|European Nucleotide Archive VANDERBILT UNIVERSITY|European Nucleotide Archive 2 0.92578 0.92449 0.12979 0.12632 0.69666 0.69775 0.51379 0.51557 151 151 B B biological fallback assumption illumina novaseq_era unknown poly_a trueseq bulk unknown unknown   United States 2023-07-14 Adult Adult Brain Nervous System
11719 11719 ERR11282339 ERX10689982 ERS15395527 ERP146895 PRJEB61813 RNA seq of brain tissues of WT and abcb4 knockout zebrafish at 2 month and 30 month E-MTAB-12901 Other The ATP binding cassette subfamily B member 1 ABCB1 encoding a multidrug transporter P glycoprotein plays a critical role in the efflux of xenobiotics in humans and is implicated in cancer resistance to chemotherapy—however little information regarding Pgp at the zebrafish. In addition to study the function of Pgp in the zebrafish brain in the aging process we performed RNA seq using brain tissue of WT and abcb4 knockout zebrafish at different ages such as 2 month and 30 month. ENA FIRST PUBLIC:2023 07 14|ENA LAST UPDATE:2023 07 14   Protocols: brain tissue were dissected from zebrafish and snap frozen until RNA isolation Trizol + zymo RNA clean column kit TruSeq RNA sample prep kit Illumina to prepare cDNA libraries post PolyA selection Sample 10 SAMEA113400381 VANDERBILT UNIVERSITY ENA FIRST PUBLIC:2023 07 14T00:23:41Z|ENA LAST UPDATE:2023 07 14T00:23:41Z|External Id:SAMEA113400381|INSDC center alias:VANDERBILT UNIVERSITY|INSDC center name:VANDERBILT UNIVERSITY|INSDC first public:2023 07 14T00:23:41Z|INSDC last update:2023 07 14T00:23:41Z|INSDC status:public|Submitter Id:E MTAB 12901:Sample 10|age:2|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|disease:normal|genotype:abcb4 / |organism part:brain|sample name:E MTAB 12901:Sample 10|scientific name:Danio rerio|sex:female|strain:AB                 Illumina NovaSeq 6000 paired end sequencing; RNA seq of brain tissues of WT and abcb4 knockout zebrafish at 2 month and 30 month E MTAB 12901:Sample 10 p Sample 10 p RNA seq of brain tissues of WT and abcb4 knockout zebrafish at 2 month and 30 month brain tissue were dissected from zebrafish and snap frozen until RNA isolation Trizol + zymo RNA clean column kit TruSeq RNA sample prep kit Illumina to prepare cDNA libraries post PolyA selection Experimental Factor: genotype:abcb4 / RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina NovaSeq 6000   ERP146895 Illumina NovaSeq 6000 paired end sequencing; RNA seq of brain tissues of WT and abcb4 knockout zebrafish at 2 month and 30 month ENA FIRST PUBLIC:2023 07 14|ENA LAST UPDATE:2023 07 14 8680-JP-0010_S1_L005_R1_001.fastq.gz 8680-JP-0010_S1_L005_R2_001.fastq.gz fastq fastq 21517298566.0 71249333.0 E MTAB 12901:8680 JP 0010 S1 L005 R 0:151 1:151 A:5890133399;C:4693794003;G:5193013881;T:5709021540;N:31335743 151 151     5890133399 4693794003 5193013881 5709021540 31335743 ERX10689982 ERS15395527 ERA23235100 VANDERBILT UNIVERSITY|European Nucleotide Archive VANDERBILT UNIVERSITY|European Nucleotide Archive 2 0.92431 0.92248 0.13476 0.13116 0.70203 0.70283 0.50087 0.50123 151 151 B B biological fallback assumption illumina novaseq_era unknown poly_a trueseq bulk unknown unknown   United States 2023-07-14 Adult Adult Brain Nervous System
11720 11720 ERR11282338 ERX10689981 ERS15395526 ERP146895 PRJEB61813 RNA seq of brain tissues of WT and abcb4 knockout zebrafish at 2 month and 30 month E-MTAB-12901 Other The ATP binding cassette subfamily B member 1 ABCB1 encoding a multidrug transporter P glycoprotein plays a critical role in the efflux of xenobiotics in humans and is implicated in cancer resistance to chemotherapy—however little information regarding Pgp at the zebrafish. In addition to study the function of Pgp in the zebrafish brain in the aging process we performed RNA seq using brain tissue of WT and abcb4 knockout zebrafish at different ages such as 2 month and 30 month. ENA FIRST PUBLIC:2023 07 14|ENA LAST UPDATE:2023 07 14   Protocols: brain tissue were dissected from zebrafish and snap frozen until RNA isolation Trizol + zymo RNA clean column kit TruSeq RNA sample prep kit Illumina to prepare cDNA libraries post PolyA selection Sample 5 SAMEA113400380 VANDERBILT UNIVERSITY ENA FIRST PUBLIC:2023 07 14T00:23:41Z|ENA LAST UPDATE:2023 07 14T00:23:41Z|External Id:SAMEA113400380|INSDC center alias:VANDERBILT UNIVERSITY|INSDC center name:VANDERBILT UNIVERSITY|INSDC first public:2023 07 14T00:23:41Z|INSDC last update:2023 07 14T00:23:41Z|INSDC status:public|Submitter Id:E MTAB 12901:Sample 5|age:30|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|disease:normal|genotype:abcb4 / |organism part:brain|sample name:E MTAB 12901:Sample 5|scientific name:Danio rerio|sex:female|strain:AB                 Illumina NovaSeq 6000 paired end sequencing; RNA seq of brain tissues of WT and abcb4 knockout zebrafish at 2 month and 30 month E MTAB 12901:Sample 5 p Sample 5 p RNA seq of brain tissues of WT and abcb4 knockout zebrafish at 2 month and 30 month brain tissue were dissected from zebrafish and snap frozen until RNA isolation Trizol + zymo RNA clean column kit TruSeq RNA sample prep kit Illumina to prepare cDNA libraries post PolyA selection Experimental Factor: genotype:abcb4 / RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina NovaSeq 6000   ERP146895 Illumina NovaSeq 6000 paired end sequencing; RNA seq of brain tissues of WT and abcb4 knockout zebrafish at 2 month and 30 month ENA FIRST PUBLIC:2023 07 14|ENA LAST UPDATE:2023 07 14 8680-JP-0005_S1_L005_R1_001.fastq.gz 8680-JP-0005_S1_L005_R2_001.fastq.gz fastq fastq 25670665306.0 85002203.0 E MTAB 12901:8680 JP 0005 S1 L005 R 0:151 1:151 A:7004508034;C:5563021332;G:6252648683;T:6813680645;N:36806612 151 151     7004508034 5563021332 6252648683 6813680645 36806612 ERX10689981 ERS15395526 ERA23235100 VANDERBILT UNIVERSITY|European Nucleotide Archive VANDERBILT UNIVERSITY|European Nucleotide Archive 2 0.92268 0.92095 0.13119 0.12796 0.70234 0.70276 0.51658 0.51519 151 151 B B biological fallback assumption illumina novaseq_era unknown poly_a trueseq bulk unknown unknown   United States 2023-07-14 Adult Adult Brain Nervous System
11721 11721 ERR11282337 ERX10689980 ERS15395525 ERP146895 PRJEB61813 RNA seq of brain tissues of WT and abcb4 knockout zebrafish at 2 month and 30 month E-MTAB-12901 Other The ATP binding cassette subfamily B member 1 ABCB1 encoding a multidrug transporter P glycoprotein plays a critical role in the efflux of xenobiotics in humans and is implicated in cancer resistance to chemotherapy—however little information regarding Pgp at the zebrafish. In addition to study the function of Pgp in the zebrafish brain in the aging process we performed RNA seq using brain tissue of WT and abcb4 knockout zebrafish at different ages such as 2 month and 30 month. ENA FIRST PUBLIC:2023 07 14|ENA LAST UPDATE:2023 07 14   Protocols: brain tissue were dissected from zebrafish and snap frozen until RNA isolation Trizol + zymo RNA clean column kit TruSeq RNA sample prep kit Illumina to prepare cDNA libraries post PolyA selection Sample 4 SAMEA113400379 VANDERBILT UNIVERSITY ENA FIRST PUBLIC:2023 07 14T00:23:41Z|ENA LAST UPDATE:2023 07 14T00:23:41Z|External Id:SAMEA113400379|INSDC center alias:VANDERBILT UNIVERSITY|INSDC center name:VANDERBILT UNIVERSITY|INSDC first public:2023 07 14T00:23:41Z|INSDC last update:2023 07 14T00:23:41Z|INSDC status:public|Submitter Id:E MTAB 12901:Sample 4|age:30|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|disease:normal|genotype:abcb4 / |organism part:brain|sample name:E MTAB 12901:Sample 4|scientific name:Danio rerio|sex:female|strain:AB                 Illumina NovaSeq 6000 paired end sequencing; RNA seq of brain tissues of WT and abcb4 knockout zebrafish at 2 month and 30 month E MTAB 12901:Sample 4 p Sample 4 p RNA seq of brain tissues of WT and abcb4 knockout zebrafish at 2 month and 30 month brain tissue were dissected from zebrafish and snap frozen until RNA isolation Trizol + zymo RNA clean column kit TruSeq RNA sample prep kit Illumina to prepare cDNA libraries post PolyA selection Experimental Factor: genotype:abcb4 / RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina NovaSeq 6000   ERP146895 Illumina NovaSeq 6000 paired end sequencing; RNA seq of brain tissues of WT and abcb4 knockout zebrafish at 2 month and 30 month ENA FIRST PUBLIC:2023 07 14|ENA LAST UPDATE:2023 07 14 8680-JP-0004_S1_L005_R1_001.fastq.gz 8680-JP-0004_S1_L005_R2_001.fastq.gz fastq fastq 19678467074.0 65160487.0 E MTAB 12901:8680 JP 0004 S1 L005 R 0:151 1:151 A:5388172969;C:4290008191;G:4741082570;T:5230699181;N:28504163 151 151     5388172969 4290008191 4741082570 5230699181 28504163 ERX10689980 ERS15395525 ERA23235100 VANDERBILT UNIVERSITY|European Nucleotide Archive VANDERBILT UNIVERSITY|European Nucleotide Archive 2 0.92019 0.91807 0.13779 0.13325 0.69739 0.69682 0.48956 0.48353 151 151 B B biological fallback assumption illumina novaseq_era unknown poly_a trueseq bulk unknown unknown   United States 2023-07-14 Adult Adult Brain Nervous System
11722 11722 ERR11282336 ERX10689979 ERS15395524 ERP146895 PRJEB61813 RNA seq of brain tissues of WT and abcb4 knockout zebrafish at 2 month and 30 month E-MTAB-12901 Other The ATP binding cassette subfamily B member 1 ABCB1 encoding a multidrug transporter P glycoprotein plays a critical role in the efflux of xenobiotics in humans and is implicated in cancer resistance to chemotherapy—however little information regarding Pgp at the zebrafish. In addition to study the function of Pgp in the zebrafish brain in the aging process we performed RNA seq using brain tissue of WT and abcb4 knockout zebrafish at different ages such as 2 month and 30 month. ENA FIRST PUBLIC:2023 07 14|ENA LAST UPDATE:2023 07 14   Protocols: brain tissue were dissected from zebrafish and snap frozen until RNA isolation Trizol + zymo RNA clean column kit TruSeq RNA sample prep kit Illumina to prepare cDNA libraries post PolyA selection Sample 12 SAMEA113400378 VANDERBILT UNIVERSITY ENA FIRST PUBLIC:2023 07 14T00:23:41Z|ENA LAST UPDATE:2023 07 14T00:23:41Z|External Id:SAMEA113400378|INSDC center alias:VANDERBILT UNIVERSITY|INSDC center name:VANDERBILT UNIVERSITY|INSDC first public:2023 07 14T00:23:41Z|INSDC last update:2023 07 14T00:23:41Z|INSDC status:public|Submitter Id:E MTAB 12901:Sample 12|age:30|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|disease:normal|genotype:abcb4 / |organism part:brain|sample name:E MTAB 12901:Sample 12|scientific name:Danio rerio|sex:female|strain:AB                 Illumina NovaSeq 6000 paired end sequencing; RNA seq of brain tissues of WT and abcb4 knockout zebrafish at 2 month and 30 month E MTAB 12901:Sample 12 p Sample 12 p RNA seq of brain tissues of WT and abcb4 knockout zebrafish at 2 month and 30 month brain tissue were dissected from zebrafish and snap frozen until RNA isolation Trizol + zymo RNA clean column kit TruSeq RNA sample prep kit Illumina to prepare cDNA libraries post PolyA selection Experimental Factor: genotype:abcb4 / RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina NovaSeq 6000   ERP146895 Illumina NovaSeq 6000 paired end sequencing; RNA seq of brain tissues of WT and abcb4 knockout zebrafish at 2 month and 30 month ENA FIRST PUBLIC:2023 07 14|ENA LAST UPDATE:2023 07 14 8680-JP-0012_S1_L005_R1_001.fastq.gz 8680-JP-0012_S1_L005_R2_001.fastq.gz fastq fastq 19025354324.0 62997862.0 E MTAB 12901:8680 JP 0012 S1 L005 R 0:151 1:151 A:5203524540;C:4195830684;G:4551047863;T:5047420365;N:27530872 151 151     5203524540 4195830684 4551047863 5047420365 27530872 ERX10689979 ERS15395524 ERA23235100 VANDERBILT UNIVERSITY|European Nucleotide Archive VANDERBILT UNIVERSITY|European Nucleotide Archive 2 0.92091 0.92095 0.13145 0.12737 0.69238 0.69258 0.50372 0.48431 151 151 B B biological fallback assumption illumina novaseq_era unknown poly_a trueseq bulk unknown unknown   United States 2023-07-14 Adult Adult Brain Nervous System
11813 11813 ERR11788863 ERX11187331 ERS16221143 ERP149944 PRJEB64780 A single cell transcriptomic atlas reveals a new myeloid parenchymal population in the zebrafish brain E-MTAB-13223 Transcriptome Analysis To understand the cellular basis of the immune compartment of the zebrafish brain we have established reliable protocols for dissociation and prospective isolation of brain leukocytes using fluorescent transgenic lines. By combining this approach with single cell RNA sequencing we have generated a gene expression atlas composed of the distinct immune cells present in the homeostatic brain. These analyses revealed the presence of subpopulations of mononuclear phagocytes and other leukocytes including cell types that have not been or have been poorly characterized so far. Here we present the characterization of a new mononuclear phagocyte population that represents an important fraction among all brain leukocytes. Adult brain single cell suspensions were prepared from adult Tgp2ry12:GFP; cd45:DsRed fish n=3 and a total of 14 000 cd45:DsRed+ cells were processed for single cell profiling using the 10x Genomics platform. ENA FIRST PUBLIC:2023 12 25|ENA LAST UPDATE:2023 12 25   Protocols: FACS sorting of cd45:Dsred cells. Brains from adult Tgp2ry12:GFP; cd45:DsRed fish n=3 were dissected in 0.9X Dulbecco′s Phosphate Buffered Saline DPBS were triturated and treated with Liberase TM at 33°C for 30 45 minutes fully dissociated using a syringe with a 26G needle and washed in 2% fetal bovine serum diluted in 0.9X DPBS. Cell suspensions were centrifuged at 290g 4ºC 10 min and filtered through a 40µm nylon mesh. Just before flow cytometry analysis using calcein violet to exclude dead cells 1µM Thermo Fisher. Flow cytometry acquisition and cell sorting was performed on a FACS ARIA II Becton Dickinson. RNA Illumina Sample 1 SAMEA114235870 Universite Libre de Bruxelles ENA FIRST PUBLIC:2023 12 25T01:15:29Z|ENA LAST UPDATE:2023 12 25T01:15:29Z|External Id:SAMEA114235870|INSDC center name:Universite Libre de Bruxelles|INSDC first public:2023 12 25T01:15:29Z|INSDC last update:2023 12 25T01:15:29Z|INSDC status:public|Submitter Id:E MTAB 13223:Sample 1|age:5|broker name:ArrayExpress|cell type:leukocyte|collection date:not collected|common name:zebrafish|developmental stage:adult|genotype:wild type genotype|geographic location country and/or sea:not collected|individual:pool of 3 fish|isolate:not applicable|organism part:brain|sample name:E MTAB 13223:Sample 1|scientific name:Danio rerio|sex:male and female|strain:AB                 A single cell transcriptomic atlas reveals a new myeloid parenchymal population in the zebrafish brain E MTAB 13223:Sample 1 p Sample 1 p A single cell transcriptomic atlas reveals a new myeloid parenchymal population in the zebrafish brain FACS sorting of cd45:Dsred cells. Brains from adult Tgp2ry12:GFP; cd45:DsRed fish n=3 were dissected in 0.9X Dulbecco′s Phosphate Buffered Saline DPBS were triturated and treated with Liberase TM at 33°C for 30 45 minutes fully dissociated using a syringe with a 26G needle and washed in 2% fetal bovine serum diluted in 0.9X DPBS. Cell suspensions were centrifuged at 290g 4ºC 10 min and filtered through a 40µm nylon mesh. Just before flow cytometry analysis using calcein violet to exclude dead cells 1µM Thermo Fisher. Flow cytometry acquisition and cell sorting was performed on a FACS ARIA II Becton Dickinson. RNA Illumina   RNA-Seq TRANSCRIPTOMIC SINGLE CELL PolyA PAIRED ILLUMINA NextSeq 550   ERP149944 NextSeq 550 paired end sequencing; A single cell transcriptomic atlas reveals a new myeloid parenchymal population in the zebrafish brain ENA FIRST PUBLIC:2023 12 25|ENA LAST UPDATE:2023 12 25 L41805_Track-81843_R1.fastq.gz L41805_Track-81843_R2.fastq.gz fastq fastq 5545444464.0 66017196.0 E MTAB 13223:L41805 Track 81843 0:28 1:56 A:1576174024;C:1193175858;G:1221776615;T:1551009004;N:3308963 28 56     1576174024 1193175858 1221776615 1551009004 3308963 ERX11187331 ERS16221143 ERA26318932 European Bioinformatics Institute|European Nucleotide Archive European Bioinformatics Institute|European Nucleotide Archive 2 0.00738 0.90151 0.00285 0.20553 0.99072 0.82402 0.4645 0.53817 28 56 T B sc-like readlen illumina nextseq unknown poly_a unknown sc single_cell_droplet 10x   United Kingdom 2023-12-25 Adult Adult Brain Nervous System
11814 11814 ERR11788864 ERX11187331 ERS16221143 ERP149944 PRJEB64780 A single cell transcriptomic atlas reveals a new myeloid parenchymal population in the zebrafish brain E-MTAB-13223 Transcriptome Analysis To understand the cellular basis of the immune compartment of the zebrafish brain we have established reliable protocols for dissociation and prospective isolation of brain leukocytes using fluorescent transgenic lines. By combining this approach with single cell RNA sequencing we have generated a gene expression atlas composed of the distinct immune cells present in the homeostatic brain. These analyses revealed the presence of subpopulations of mononuclear phagocytes and other leukocytes including cell types that have not been or have been poorly characterized so far. Here we present the characterization of a new mononuclear phagocyte population that represents an important fraction among all brain leukocytes. Adult brain single cell suspensions were prepared from adult Tgp2ry12:GFP; cd45:DsRed fish n=3 and a total of 14 000 cd45:DsRed+ cells were processed for single cell profiling using the 10x Genomics platform. ENA FIRST PUBLIC:2023 12 25|ENA LAST UPDATE:2023 12 25   Protocols: FACS sorting of cd45:Dsred cells. Brains from adult Tgp2ry12:GFP; cd45:DsRed fish n=3 were dissected in 0.9X Dulbecco′s Phosphate Buffered Saline DPBS were triturated and treated with Liberase TM at 33°C for 30 45 minutes fully dissociated using a syringe with a 26G needle and washed in 2% fetal bovine serum diluted in 0.9X DPBS. Cell suspensions were centrifuged at 290g 4ºC 10 min and filtered through a 40µm nylon mesh. Just before flow cytometry analysis using calcein violet to exclude dead cells 1µM Thermo Fisher. Flow cytometry acquisition and cell sorting was performed on a FACS ARIA II Becton Dickinson. RNA Illumina Sample 1 SAMEA114235870 Universite Libre de Bruxelles ENA FIRST PUBLIC:2023 12 25T01:15:29Z|ENA LAST UPDATE:2023 12 25T01:15:29Z|External Id:SAMEA114235870|INSDC center name:Universite Libre de Bruxelles|INSDC first public:2023 12 25T01:15:29Z|INSDC last update:2023 12 25T01:15:29Z|INSDC status:public|Submitter Id:E MTAB 13223:Sample 1|age:5|broker name:ArrayExpress|cell type:leukocyte|collection date:not collected|common name:zebrafish|developmental stage:adult|genotype:wild type genotype|geographic location country and/or sea:not collected|individual:pool of 3 fish|isolate:not applicable|organism part:brain|sample name:E MTAB 13223:Sample 1|scientific name:Danio rerio|sex:male and female|strain:AB                 A single cell transcriptomic atlas reveals a new myeloid parenchymal population in the zebrafish brain E MTAB 13223:Sample 1 p Sample 1 p A single cell transcriptomic atlas reveals a new myeloid parenchymal population in the zebrafish brain FACS sorting of cd45:Dsred cells. Brains from adult Tgp2ry12:GFP; cd45:DsRed fish n=3 were dissected in 0.9X Dulbecco′s Phosphate Buffered Saline DPBS were triturated and treated with Liberase TM at 33°C for 30 45 minutes fully dissociated using a syringe with a 26G needle and washed in 2% fetal bovine serum diluted in 0.9X DPBS. Cell suspensions were centrifuged at 290g 4ºC 10 min and filtered through a 40µm nylon mesh. Just before flow cytometry analysis using calcein violet to exclude dead cells 1µM Thermo Fisher. Flow cytometry acquisition and cell sorting was performed on a FACS ARIA II Becton Dickinson. RNA Illumina   RNA-Seq TRANSCRIPTOMIC SINGLE CELL PolyA PAIRED ILLUMINA NextSeq 550   ERP149944 NextSeq 550 paired end sequencing; A single cell transcriptomic atlas reveals a new myeloid parenchymal population in the zebrafish brain ENA FIRST PUBLIC:2023 12 25|ENA LAST UPDATE:2023 12 25 L41805_Track-81377_R1.fastq.gz L41805_Track-81377_R2.fastq.gz fastq fastq 3184137096.0 37906394.0 E MTAB 13223:L41805 Track 81377 0:28 1:56 A:899943184;C:685571078;G:703849582;T:889228559;N:5544693 28 56     899943184 685571078 703849582 889228559 5544693 ERX11187331 ERS16221143 ERA26318932 European Bioinformatics Institute|European Nucleotide Archive European Bioinformatics Institute|European Nucleotide Archive 2 0.00734 0.90077 0.00293 0.20318 0.99064 0.82765 0.47994 0.53473 28 56 T B sc-like readlen illumina nextseq unknown poly_a unknown sc single_cell_droplet 10x   United Kingdom 2023-12-25 Adult Adult Brain Nervous System
31902 31902 SRR28763293 SRX24328715 SRS21090019 SRP503258 PRJNA1102381 Acidification alters anxiety like behaviour and brain gene expression in zebrafish PRJNA1102381 Other CO2 driven acidification of freshwater ecosystems is an accelerating process that can impact aquatic life in the future. Despite their importance in accurately predicting how future freshwater teleost fish will respond to changing environments molecular mechanisms coordinating the response to elevated CO2 have not been extensively studied. Making use of the zebrafish Danio rerio considered 'tolerant' to acidified waters we analyzed the behavioural and transcriptomic response to acidification to highlight potential response mechanisms in the brain and the gills which mediate behaviour and perform acid base regulation. Adult zebrafish were exposed to control 500 uatm and elevated CO2 1000 uatm for five days and submitted to Open Field and Novel Object Approach tests revealing a decrease in anxiety like behaviour in response to elevated CO2. In the brain acidification caused differential expression of genes involved in cytoskeletal organization cellular transport immunity and the visual neural system. In the gills an elevated expression of genes involved in immune response and oxidoreduction with increasing pCO2 in combination with decreased anxiety like behaviour point to an increase of oxidative stress. However the absence of differentially expressed genes in gills indicates that this species has adapted to regulating its acid base balance in acidified waters common in its natural habitat. Our study pinpoints the molecular response to elevated CO2 possibly triggering behavioural changes in zebrafish and suggest that not only marine but also freshwater fishes will be behaviourally affected by acidification due to rapid climate change.         EB19   replicate name:10|breed:AB|age:4 months|dev stage:adult|collection date:2021 11 14|geo loc name:Hong Kong|sex:not determined|tissue:brain|BioSampleModel:Model organism or animal                 RNA Seq of 4mpf Danio rerio brain EB19 EB19 Total RNA was extracted using the RNeasy Micro Kit Qiagen.     RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina NovaSeq 6000   SRP503258     EB19_1.fastq.gz EB19_2.fastq.gz fastq fastq 9436386526.0 31246313.0 EB19 1.fastq.gz 0:151 1:151 A:2716315062;C:1976294752;G:2147671436;T:2595980360;N:124916 151 151     2716315062 1976294752 2147671436 2595980360 124916 SRX24328715 SRS21090019 SRA1849282 The University of Hong Kong|School of Biological Sciences The University of Hong Kong 2 0.91409 0.91345 0.24896 0.24788 0.78056 0.78279 0.72332 0.73168 151 151 B B biological fallback assumption illumina novaseq_era unknown poly_a unknown bulk unknown unknown   China 2024-04-22 Adult Adult Brain Nervous System
31903 31903 SRR28763294 SRX24328714 SRS21090018 SRP503258 PRJNA1102381 Acidification alters anxiety like behaviour and brain gene expression in zebrafish PRJNA1102381 Other CO2 driven acidification of freshwater ecosystems is an accelerating process that can impact aquatic life in the future. Despite their importance in accurately predicting how future freshwater teleost fish will respond to changing environments molecular mechanisms coordinating the response to elevated CO2 have not been extensively studied. Making use of the zebrafish Danio rerio considered 'tolerant' to acidified waters we analyzed the behavioural and transcriptomic response to acidification to highlight potential response mechanisms in the brain and the gills which mediate behaviour and perform acid base regulation. Adult zebrafish were exposed to control 500 uatm and elevated CO2 1000 uatm for five days and submitted to Open Field and Novel Object Approach tests revealing a decrease in anxiety like behaviour in response to elevated CO2. In the brain acidification caused differential expression of genes involved in cytoskeletal organization cellular transport immunity and the visual neural system. In the gills an elevated expression of genes involved in immune response and oxidoreduction with increasing pCO2 in combination with decreased anxiety like behaviour point to an increase of oxidative stress. However the absence of differentially expressed genes in gills indicates that this species has adapted to regulating its acid base balance in acidified waters common in its natural habitat. Our study pinpoints the molecular response to elevated CO2 possibly triggering behavioural changes in zebrafish and suggest that not only marine but also freshwater fishes will be behaviourally affected by acidification due to rapid climate change.         EB18   replicate name:9|breed:AB|age:4 months|dev stage:adult|collection date:2021 11 08|geo loc name:Hong Kong|sex:not determined|tissue:brain|BioSampleModel:Model organism or animal                 RNA Seq of 4mpf Danio rerio brain EB18 EB18 Total RNA was extracted using the RNeasy Micro Kit Qiagen.     RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina NovaSeq 6000   SRP503258     EB18_1.fastq.gz EB18_2.fastq.gz fastq fastq 9118420390.0 30193445.0 EB18 1.fastq.gz 0:151 1:151 A:2629035452;C:1916588061;G:2003273132;T:2569401842;N:121903 151 151     2629035452 1916588061 2003273132 2569401842 121903 SRX24328714 SRS21090018 SRA1849282 The University of Hong Kong|School of Biological Sciences The University of Hong Kong 2 0.92136 0.91989 0.22403 0.22513 0.72318 0.72344 0.58479 0.59137 151 151 B B biological fallback assumption illumina novaseq_era unknown poly_a unknown bulk unknown unknown   China 2024-04-22 Adult Adult Brain Nervous System

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CREATE TABLE run_metadata("run.accession" VARCHAR, "experiment.accession" VARCHAR, "sample.accession" VARCHAR, "study.accession" VARCHAR, bioproject VARCHAR, "study.title" VARCHAR, "study.alias" VARCHAR, "study.type" VARCHAR, "study.abstract" VARCHAR, "study.attributes" VARCHAR, "study.PMIDs" VARCHAR, "sample.description" VARCHAR, "sample.title" VARCHAR, "sample.alias" VARCHAR, "sample.centername" VARCHAR, "sample.attributes" VARCHAR, "GEOsample.title" VARCHAR, "GEOsample.dataprocessing" VARCHAR, "GEOsample.source" VARCHAR, "GEOsample.treatmentprotocol" VARCHAR, "GEOsample.extractprotocol" VARCHAR, "GEOsample.growthprotocol" VARCHAR, "GEOsample.characteristics" VARCHAR, "GEOsample.accession" VARCHAR, "experiment.title" VARCHAR, "experiment.alias" VARCHAR, "experiment.library_name" VARCHAR, "experiment.design_description" VARCHAR, "experiment.library_construction_protocol" VARCHAR, "experiment.attributes" VARCHAR, "experiment.library_strategy" VARCHAR, "experiment.library_source" VARCHAR, "experiment.library_selection" VARCHAR, "experiment.library_layout" VARCHAR, "experiment.platform" VARCHAR, "experiment.instrument_model" VARCHAR, "experiment.spot_descriptor" VARCHAR, "experiment.study_ref" VARCHAR, "run.title" VARCHAR, "run.attributes" VARCHAR, "run.filename" VARCHAR, "run.semantic_name" VARCHAR, "run.total_bases" DOUBLE, "run.total_spots" DOUBLE, "run.alias" VARCHAR, "run.read_lengths" VARCHAR, "run.base_counts" VARCHAR, "run.r1_length" BIGINT, "run.r2_length" BIGINT, "run.r3_length" BIGINT, "run.r4_length" BIGINT, "run.Acount" BIGINT, "run.Ccount" BIGINT, "run.Gcount" BIGINT, "run.Tcount" BIGINT, "run.Ncount" BIGINT, "run.experiment" VARCHAR, "run.pool_member" VARCHAR, "submission.accession" VARCHAR, "submission.srasource" VARCHAR, "submission.bioprojectsource" VARCHAR, "seqdetective.n_mates" BIGINT, "seqdetective.mapping_rate.mate1" DOUBLE, "seqdetective.mapping_rate.mate2" DOUBLE, "seqdetective.nofeature_rate.mate1" DOUBLE, "seqdetective.nofeature_rate.mate2" DOUBLE, "seqdetective.sparsity.mate1" DOUBLE, "seqdetective.sparsity.mate2" DOUBLE, "seqdetective.pos_strand_rate.mate1" DOUBLE, "seqdetective.pos_strand_rate.mate2" DOUBLE, "seqdetective.readlen.mate1" BIGINT, "seqdetective.readlen.mate2" BIGINT, "seqdetective.judgement.mate1" VARCHAR, "seqdetective.judgement.mate2" VARCHAR, "seqdetective.judgement.reason" VARCHAR, platform_family VARCHAR, instrument_generation VARCHAR, read_bias VARCHAR, selection_class VARCHAR, prep_kit VARCHAR, sc_or_bulk VARCHAR, tech_class VARCHAR, technology VARCHAR, tech_variant VARCHAR, "submission.bioprojectsource.country" VARCHAR, earliest_date DATE, devstage_curation VARCHAR, devstage_curation_coarse VARCHAR, tissue_curation VARCHAR, tissue_curation_coarse VARCHAR);;
CREATE INDEX idx_run_bioproject ON run_metadata(bioproject);;
CREATE INDEX idx_run_run_accession ON run_metadata("run.accession");;