run_metadata
8 rows where experiment.library_selection = "PolyA", experiment.library_source = "TRANSCRIPTOMIC SINGLE CELL" and tissue_curation = "Brain"
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| Link | rowid ▼ | run.accession | experiment.accession | sample.accession | study.accession | bioproject | study.title | study.alias | study.type | study.abstract | study.attributes | study.PMIDs | sample.description | sample.title | sample.alias | sample.centername | sample.attributes | GEOsample.title | GEOsample.dataprocessing | GEOsample.source | GEOsample.treatmentprotocol | GEOsample.extractprotocol | GEOsample.growthprotocol | GEOsample.characteristics | GEOsample.accession | experiment.title | experiment.alias | experiment.library_name | experiment.design_description | experiment.library_construction_protocol | experiment.attributes | experiment.library_strategy | experiment.library_source | experiment.library_selection | experiment.library_layout | experiment.platform | experiment.instrument_model | experiment.spot_descriptor | experiment.study_ref | run.title | run.attributes | run.filename | run.semantic_name | run.total_bases | run.total_spots | run.alias | run.read_lengths | run.base_counts | run.r1_length | run.r2_length | run.r3_length | run.r4_length | run.Acount | run.Ccount | run.Gcount | run.Tcount | run.Ncount | run.experiment | run.pool_member | submission.accession | submission.srasource | submission.bioprojectsource | seqdetective.n_mates | seqdetective.mapping_rate.mate1 | seqdetective.mapping_rate.mate2 | seqdetective.nofeature_rate.mate1 | seqdetective.nofeature_rate.mate2 | seqdetective.sparsity.mate1 | seqdetective.sparsity.mate2 | seqdetective.pos_strand_rate.mate1 | seqdetective.pos_strand_rate.mate2 | seqdetective.readlen.mate1 | seqdetective.readlen.mate2 | seqdetective.judgement.mate1 | seqdetective.judgement.mate2 | seqdetective.judgement.reason | platform_family | instrument_generation | read_bias | selection_class | prep_kit | sc_or_bulk | tech_class | technology | tech_variant | submission.bioprojectsource.country | earliest_date | devstage_curation | devstage_curation_coarse | tissue_curation | tissue_curation_coarse |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 11813 | 11813 | ERR11788863 | ERX11187331 | ERS16221143 | ERP149944 | PRJEB64780 | A single cell transcriptomic atlas reveals a new myeloid parenchymal population in the zebrafish brain | E-MTAB-13223 | Transcriptome Analysis | To understand the cellular basis of the immune compartment of the zebrafish brain we have established reliable protocols for dissociation and prospective isolation of brain leukocytes using fluorescent transgenic lines. By combining this approach with single cell RNA sequencing we have generated a gene expression atlas composed of the distinct immune cells present in the homeostatic brain. These analyses revealed the presence of subpopulations of mononuclear phagocytes and other leukocytes including cell types that have not been or have been poorly characterized so far. Here we present the characterization of a new mononuclear phagocyte population that represents an important fraction among all brain leukocytes. Adult brain single cell suspensions were prepared from adult Tgp2ry12:GFP; cd45:DsRed fish n=3 and a total of 14 000 cd45:DsRed+ cells were processed for single cell profiling using the 10x Genomics platform. | ENA FIRST PUBLIC:2023 12 25|ENA LAST UPDATE:2023 12 25 | Protocols: FACS sorting of cd45:Dsred cells. Brains from adult Tgp2ry12:GFP; cd45:DsRed fish n=3 were dissected in 0.9X Dulbecco′s Phosphate Buffered Saline DPBS were triturated and treated with Liberase TM at 33°C for 30 45 minutes fully dissociated using a syringe with a 26G needle and washed in 2% fetal bovine serum diluted in 0.9X DPBS. Cell suspensions were centrifuged at 290g 4ºC 10 min and filtered through a 40µm nylon mesh. Just before flow cytometry analysis using calcein violet to exclude dead cells 1µM Thermo Fisher. Flow cytometry acquisition and cell sorting was performed on a FACS ARIA II Becton Dickinson. RNA Illumina | Sample 1 | SAMEA114235870 | Universite Libre de Bruxelles | ENA FIRST PUBLIC:2023 12 25T01:15:29Z|ENA LAST UPDATE:2023 12 25T01:15:29Z|External Id:SAMEA114235870|INSDC center name:Universite Libre de Bruxelles|INSDC first public:2023 12 25T01:15:29Z|INSDC last update:2023 12 25T01:15:29Z|INSDC status:public|Submitter Id:E MTAB 13223:Sample 1|age:5|broker name:ArrayExpress|cell type:leukocyte|collection date:not collected|common name:zebrafish|developmental stage:adult|genotype:wild type genotype|geographic location country and/or sea:not collected|individual:pool of 3 fish|isolate:not applicable|organism part:brain|sample name:E MTAB 13223:Sample 1|scientific name:Danio rerio|sex:male and female|strain:AB | A single cell transcriptomic atlas reveals a new myeloid parenchymal population in the zebrafish brain | E MTAB 13223:Sample 1 p | Sample 1 p | A single cell transcriptomic atlas reveals a new myeloid parenchymal population in the zebrafish brain | FACS sorting of cd45:Dsred cells. Brains from adult Tgp2ry12:GFP; cd45:DsRed fish n=3 were dissected in 0.9X Dulbecco′s Phosphate Buffered Saline DPBS were triturated and treated with Liberase TM at 33°C for 30 45 minutes fully dissociated using a syringe with a 26G needle and washed in 2% fetal bovine serum diluted in 0.9X DPBS. Cell suspensions were centrifuged at 290g 4ºC 10 min and filtered through a 40µm nylon mesh. Just before flow cytometry analysis using calcein violet to exclude dead cells 1µM Thermo Fisher. Flow cytometry acquisition and cell sorting was performed on a FACS ARIA II Becton Dickinson. RNA Illumina | RNA-Seq | TRANSCRIPTOMIC SINGLE CELL | PolyA | PAIRED | ILLUMINA | NextSeq 550 | ERP149944 | NextSeq 550 paired end sequencing; A single cell transcriptomic atlas reveals a new myeloid parenchymal population in the zebrafish brain | ENA FIRST PUBLIC:2023 12 25|ENA LAST UPDATE:2023 12 25 | L41805_Track-81843_R1.fastq.gz L41805_Track-81843_R2.fastq.gz | fastq fastq | 5545444464.0 | 66017196.0 | E MTAB 13223:L41805 Track 81843 | 0:28 1:56 | A:1576174024;C:1193175858;G:1221776615;T:1551009004;N:3308963 | 28 | 56 | 1576174024 | 1193175858 | 1221776615 | 1551009004 | 3308963 | ERX11187331 | ERS16221143 | ERA26318932 | European Bioinformatics Institute|European Nucleotide Archive | European Bioinformatics Institute|European Nucleotide Archive | 2 | 0.00738 | 0.90151 | 0.00285 | 0.20553 | 0.99072 | 0.82402 | 0.4645 | 0.53817 | 28 | 56 | T | B | sc-like readlen | illumina | nextseq | unknown | poly_a | unknown | sc | single_cell_droplet | 10x | United Kingdom | 2023-12-25 | Adult | Adult | Brain | Nervous System | ||||||||||||||
| 11814 | 11814 | ERR11788864 | ERX11187331 | ERS16221143 | ERP149944 | PRJEB64780 | A single cell transcriptomic atlas reveals a new myeloid parenchymal population in the zebrafish brain | E-MTAB-13223 | Transcriptome Analysis | To understand the cellular basis of the immune compartment of the zebrafish brain we have established reliable protocols for dissociation and prospective isolation of brain leukocytes using fluorescent transgenic lines. By combining this approach with single cell RNA sequencing we have generated a gene expression atlas composed of the distinct immune cells present in the homeostatic brain. These analyses revealed the presence of subpopulations of mononuclear phagocytes and other leukocytes including cell types that have not been or have been poorly characterized so far. Here we present the characterization of a new mononuclear phagocyte population that represents an important fraction among all brain leukocytes. Adult brain single cell suspensions were prepared from adult Tgp2ry12:GFP; cd45:DsRed fish n=3 and a total of 14 000 cd45:DsRed+ cells were processed for single cell profiling using the 10x Genomics platform. | ENA FIRST PUBLIC:2023 12 25|ENA LAST UPDATE:2023 12 25 | Protocols: FACS sorting of cd45:Dsred cells. Brains from adult Tgp2ry12:GFP; cd45:DsRed fish n=3 were dissected in 0.9X Dulbecco′s Phosphate Buffered Saline DPBS were triturated and treated with Liberase TM at 33°C for 30 45 minutes fully dissociated using a syringe with a 26G needle and washed in 2% fetal bovine serum diluted in 0.9X DPBS. Cell suspensions were centrifuged at 290g 4ºC 10 min and filtered through a 40µm nylon mesh. Just before flow cytometry analysis using calcein violet to exclude dead cells 1µM Thermo Fisher. Flow cytometry acquisition and cell sorting was performed on a FACS ARIA II Becton Dickinson. RNA Illumina | Sample 1 | SAMEA114235870 | Universite Libre de Bruxelles | ENA FIRST PUBLIC:2023 12 25T01:15:29Z|ENA LAST UPDATE:2023 12 25T01:15:29Z|External Id:SAMEA114235870|INSDC center name:Universite Libre de Bruxelles|INSDC first public:2023 12 25T01:15:29Z|INSDC last update:2023 12 25T01:15:29Z|INSDC status:public|Submitter Id:E MTAB 13223:Sample 1|age:5|broker name:ArrayExpress|cell type:leukocyte|collection date:not collected|common name:zebrafish|developmental stage:adult|genotype:wild type genotype|geographic location country and/or sea:not collected|individual:pool of 3 fish|isolate:not applicable|organism part:brain|sample name:E MTAB 13223:Sample 1|scientific name:Danio rerio|sex:male and female|strain:AB | A single cell transcriptomic atlas reveals a new myeloid parenchymal population in the zebrafish brain | E MTAB 13223:Sample 1 p | Sample 1 p | A single cell transcriptomic atlas reveals a new myeloid parenchymal population in the zebrafish brain | FACS sorting of cd45:Dsred cells. Brains from adult Tgp2ry12:GFP; cd45:DsRed fish n=3 were dissected in 0.9X Dulbecco′s Phosphate Buffered Saline DPBS were triturated and treated with Liberase TM at 33°C for 30 45 minutes fully dissociated using a syringe with a 26G needle and washed in 2% fetal bovine serum diluted in 0.9X DPBS. Cell suspensions were centrifuged at 290g 4ºC 10 min and filtered through a 40µm nylon mesh. Just before flow cytometry analysis using calcein violet to exclude dead cells 1µM Thermo Fisher. Flow cytometry acquisition and cell sorting was performed on a FACS ARIA II Becton Dickinson. RNA Illumina | RNA-Seq | TRANSCRIPTOMIC SINGLE CELL | PolyA | PAIRED | ILLUMINA | NextSeq 550 | ERP149944 | NextSeq 550 paired end sequencing; A single cell transcriptomic atlas reveals a new myeloid parenchymal population in the zebrafish brain | ENA FIRST PUBLIC:2023 12 25|ENA LAST UPDATE:2023 12 25 | L41805_Track-81377_R1.fastq.gz L41805_Track-81377_R2.fastq.gz | fastq fastq | 3184137096.0 | 37906394.0 | E MTAB 13223:L41805 Track 81377 | 0:28 1:56 | A:899943184;C:685571078;G:703849582;T:889228559;N:5544693 | 28 | 56 | 899943184 | 685571078 | 703849582 | 889228559 | 5544693 | ERX11187331 | ERS16221143 | ERA26318932 | European Bioinformatics Institute|European Nucleotide Archive | European Bioinformatics Institute|European Nucleotide Archive | 2 | 0.00734 | 0.90077 | 0.00293 | 0.20318 | 0.99064 | 0.82765 | 0.47994 | 0.53473 | 28 | 56 | T | B | sc-like readlen | illumina | nextseq | unknown | poly_a | unknown | sc | single_cell_droplet | 10x | United Kingdom | 2023-12-25 | Adult | Adult | Brain | Nervous System | ||||||||||||||
| 35865 | 35865 | SRR33094447 | SRX28358130 | SRS24687221 | SRP578075 | PRJNA1249535 | P301S Mutant Tau Driven Zebrafish Platform Enables Systematic Identification of Pharmacological GSK3B Inhibitors with Anti Neurodegenerative Efficacy | PRJNA1249535 | Other | Single cell transcriptome sequencing provides high resolution transcriptome data on the molecular mechanisms by which Tau P301S mediates Alzheimer's disease like pathological changes in zebrafish providing unique insights into the pathogenesis of Tau pathology mediated cell to cell interactions in AD. | TauP301S 4 | TauP301S 4 | breed:zebrafish|age:4 month|collection date:2024 05 18|geo loc name:China:Shantou|sex:not determined|tissue:brain|BioSampleModel:Model organism or animal | P301S Mutant Tau Driven Zebrafish Platform Enables Systematic Identification of Pharmacological GSK3B Inhibitors with Anti Neurodegenerative Efficacy | TauP301S 4 | TauP301S 4 | tissue | RNA-Seq | TRANSCRIPTOMIC SINGLE CELL | PolyA | PAIRED | BGISEQ | BGISEQ-500 | SRP578075 | AD4_S1_L001_R1_001.fastq.gz AD4_S1_L001_R2_001.fastq.gz | fastq fastq | 129145897746.0 | 427635423.0 | AD4 S1 L001 R1 001.fastq.gz | 0:151 1:151 | A:37870495722;C:24917123133;G:22536537853;T:43764941649;N:56799389 | 151 | 151 | 37870495722 | 24917123133 | 22536537853 | 43764941649 | 56799389 | SRX28358130 | SRS24687221 | SRA2110625 | Shantou university medical college|Neuroscience Center | Shantou university medical college | T | B | mate1 technical by mapping diff | bgi | bgi | unknown | poly_a | unknown | sc | unknown | unknown | China | 2025-04-13 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||||||||||||||
| 35866 | 35866 | SRR33094448 | SRX28358129 | SRS24687220 | SRP578075 | PRJNA1249535 | P301S Mutant Tau Driven Zebrafish Platform Enables Systematic Identification of Pharmacological GSK3B Inhibitors with Anti Neurodegenerative Efficacy | PRJNA1249535 | Other | Single cell transcriptome sequencing provides high resolution transcriptome data on the molecular mechanisms by which Tau P301S mediates Alzheimer's disease like pathological changes in zebrafish providing unique insights into the pathogenesis of Tau pathology mediated cell to cell interactions in AD. | TauP301S 3 | TauP301S 3 | breed:zebrafish|age:4 month|collection date:2024 05 17|geo loc name:China:Shantou|sex:not determined|tissue:brain|BioSampleModel:Model organism or animal | P301S Mutant Tau Driven Zebrafish Platform Enables Systematic Identification of Pharmacological GSK3B Inhibitors with Anti Neurodegenerative Efficacy | TauP301S 3 | TauP301S 3 | tissue | RNA-Seq | TRANSCRIPTOMIC SINGLE CELL | PolyA | PAIRED | BGISEQ | BGISEQ-500 | SRP578075 | AD3_S1_L001_R1_001.fastq.gz AD3_S1_L001_R2_001.fastq.gz | fastq fastq | 130723873718.0 | 432860509.0 | AD3 S1 L001 R1 001.fastq.gz | 0:151 1:151 | A:38509426012;C:25238065771;G:22586405501;T:44332442791;N:57533643 | 151 | 151 | 38509426012 | 25238065771 | 22586405501 | 44332442791 | 57533643 | SRX28358129 | SRS24687220 | SRA2110625 | Shantou university medical college|Neuroscience Center | Shantou university medical college | T | B | mate1 technical by mapping diff | bgi | bgi | unknown | poly_a | unknown | sc | unknown | unknown | China | 2025-04-13 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||||||||||||||
| 35867 | 35867 | SRR33094449 | SRX28358128 | SRS24687219 | SRP578075 | PRJNA1249535 | P301S Mutant Tau Driven Zebrafish Platform Enables Systematic Identification of Pharmacological GSK3B Inhibitors with Anti Neurodegenerative Efficacy | PRJNA1249535 | Other | Single cell transcriptome sequencing provides high resolution transcriptome data on the molecular mechanisms by which Tau P301S mediates Alzheimer's disease like pathological changes in zebrafish providing unique insights into the pathogenesis of Tau pathology mediated cell to cell interactions in AD. | TauP301S 2 | TauP301S 2 | breed:zebrafish|age:4 month|collection date:2024 05 16|geo loc name:China:Shantou|sex:not determined|tissue:brain|BioSampleModel:Model organism or animal | P301S Mutant Tau Driven Zebrafish Platform Enables Systematic Identification of Pharmacological GSK3B Inhibitors with Anti Neurodegenerative Efficacy | TauP301S 2 | TauP301S 2 | tissue | RNA-Seq | TRANSCRIPTOMIC SINGLE CELL | PolyA | PAIRED | BGISEQ | BGISEQ-500 | SRP578075 | AD2_S1_L001_R1_001.fastq.gz AD2_S1_L001_R2_001.fastq.gz | fastq fastq | 130145208498.0 | 430944399.0 | AD2 S1 L001 R1 001.fastq.gz | 0:151 1:151 | A:37802929660;C:22980541562;G:20982421471;T:48304142442;N:75173363 | 151 | 151 | 37802929660 | 22980541562 | 20982421471 | 48304142442 | 75173363 | SRX28358128 | SRS24687219 | SRA2110625 | Shantou university medical college|Neuroscience Center | Shantou university medical college | T | B | mate1 technical by mapping diff | bgi | bgi | unknown | poly_a | unknown | sc | unknown | unknown | China | 2025-04-13 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||||||||||||||
| 35868 | 35868 | SRR33094450 | SRX28358127 | SRS24687218 | SRP578075 | PRJNA1249535 | P301S Mutant Tau Driven Zebrafish Platform Enables Systematic Identification of Pharmacological GSK3B Inhibitors with Anti Neurodegenerative Efficacy | PRJNA1249535 | Other | Single cell transcriptome sequencing provides high resolution transcriptome data on the molecular mechanisms by which Tau P301S mediates Alzheimer's disease like pathological changes in zebrafish providing unique insights into the pathogenesis of Tau pathology mediated cell to cell interactions in AD. | TauP301S 1 | TauP301S 1 | breed:zebrafish|age:4 month|collection date:2024 05 15|geo loc name:China:Shantou|sex:not determined|tissue:brain|BioSampleModel:Model organism or animal | P301S Mutant Tau Driven Zebrafish Platform Enables Systematic Identification of Pharmacological GSK3B Inhibitors with Anti Neurodegenerative Efficacy | TauP301S 1 | TauP301S 1 | tissue | RNA-Seq | TRANSCRIPTOMIC SINGLE CELL | PolyA | PAIRED | BGISEQ | BGISEQ-500 | SRP578075 | AD1_S1_L001_R1_001.fastq.gz AD1_S1_L001_R2_001.fastq.gz | fastq fastq | 151897861402.0 | 502973051.0 | AD1 S1 L001 R1 001.fastq.gz | 0:151 1:151 | A:44290580650;C:26864167508;G:24611898618;T:56043760812;N:87453814 | 151 | 151 | 44290580650 | 26864167508 | 24611898618 | 56043760812 | 87453814 | SRX28358127 | SRS24687218 | SRA2110625 | Shantou university medical college|Neuroscience Center | Shantou university medical college | T | B | mate1 technical by mapping diff | bgi | bgi | unknown | poly_a | unknown | sc | unknown | unknown | China | 2025-04-13 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||||||||||||||
| 35869 | 35869 | SRR33094451 | SRX28358126 | SRS24687216 | SRP578075 | PRJNA1249535 | P301S Mutant Tau Driven Zebrafish Platform Enables Systematic Identification of Pharmacological GSK3B Inhibitors with Anti Neurodegenerative Efficacy | PRJNA1249535 | Other | Single cell transcriptome sequencing provides high resolution transcriptome data on the molecular mechanisms by which Tau P301S mediates Alzheimer's disease like pathological changes in zebrafish providing unique insights into the pathogenesis of Tau pathology mediated cell to cell interactions in AD. | Wildtype2 | Wildtype2 | breed:zebrafish|age:4 month|collection date:2024 05 14|geo loc name:China:Shantou|sex:not determined|tissue:brain|BioSampleModel:Model organism or animal | P301S Mutant Tau Driven Zebrafish Platform Enables Systematic Identification of Pharmacological GSK3B Inhibitors with Anti Neurodegenerative Efficacy | Wildtype2 | Wildtype2 | tissue | RNA-Seq | TRANSCRIPTOMIC SINGLE CELL | PolyA | SINGLE | BGISEQ | BGISEQ-500 | SRP578075 | WT2_S1_L001_I1_001.fastq.gz | fastq | 15183539264.0 | 1897942408.0 | WT2 S1 L001 I1 001.fastq.gz | 0:8 | A:5024041949;C:2632487194;G:2782260146;T:4744243552;N:506423 | 8 | 5024041949 | 2632487194 | 2782260146 | 4744243552 | 506423 | SRX28358126 | SRS24687216 | SRA2110625 | Shantou university medical college|Neuroscience Center | Shantou university medical college | T | under 1.2% mapping rate | bgi | bgi | unknown | poly_a | unknown | sc | unknown | unknown | China | 2025-04-18 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||||||||||||||||
| 35870 | 35870 | SRR33094452 | SRX28358125 | SRS24687217 | SRP578075 | PRJNA1249535 | P301S Mutant Tau Driven Zebrafish Platform Enables Systematic Identification of Pharmacological GSK3B Inhibitors with Anti Neurodegenerative Efficacy | PRJNA1249535 | Other | Single cell transcriptome sequencing provides high resolution transcriptome data on the molecular mechanisms by which Tau P301S mediates Alzheimer's disease like pathological changes in zebrafish providing unique insights into the pathogenesis of Tau pathology mediated cell to cell interactions in AD. | Wildtype1 | Wildtype1 | breed:zebrafish|age:4 month|collection date:2024 05 13|geo loc name:China:Shantou|sex:not determined|tissue:brain|BioSampleModel:Model organism or animal | P301S Mutant Tau Driven Zebrafish Platform Enables Systematic Identification of Pharmacological GSK3B Inhibitors with Anti Neurodegenerative Efficacy | Wildtype1 | Wildtype1 | tissue | RNA-Seq | TRANSCRIPTOMIC SINGLE CELL | PolyA | SINGLE | BGISEQ | BGISEQ-500 | SRP578075 | WT1_S1_L001_I1_001.fastq.gz | fastq | 7991376264.0 | 998922033.0 | WT1 S1 L001 I1 001.fastq.gz | 0:8 | A:2573734851;C:1426474966;G:1492966198;T:2498173074;N:27175 | 8 | 2573734851 | 1426474966 | 1492966198 | 2498173074 | 27175 | SRX28358125 | SRS24687217 | SRA2110625 | Shantou university medical college|Neuroscience Center | Shantou university medical college | T | under 1.2% mapping rate | bgi | bgi | unknown | poly_a | unknown | sc | unknown | unknown | China | 2025-04-18 | Adult | Adult | Brain | Nervous System |
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CREATE TABLE run_metadata("run.accession" VARCHAR, "experiment.accession" VARCHAR, "sample.accession" VARCHAR, "study.accession" VARCHAR, bioproject VARCHAR, "study.title" VARCHAR, "study.alias" VARCHAR, "study.type" VARCHAR, "study.abstract" VARCHAR, "study.attributes" VARCHAR, "study.PMIDs" VARCHAR, "sample.description" VARCHAR, "sample.title" VARCHAR, "sample.alias" VARCHAR, "sample.centername" VARCHAR, "sample.attributes" VARCHAR, "GEOsample.title" VARCHAR, "GEOsample.dataprocessing" VARCHAR, "GEOsample.source" VARCHAR, "GEOsample.treatmentprotocol" VARCHAR, "GEOsample.extractprotocol" VARCHAR, "GEOsample.growthprotocol" VARCHAR, "GEOsample.characteristics" VARCHAR, "GEOsample.accession" VARCHAR, "experiment.title" VARCHAR, "experiment.alias" VARCHAR, "experiment.library_name" VARCHAR, "experiment.design_description" VARCHAR, "experiment.library_construction_protocol" VARCHAR, "experiment.attributes" VARCHAR, "experiment.library_strategy" VARCHAR, "experiment.library_source" VARCHAR, "experiment.library_selection" VARCHAR, "experiment.library_layout" VARCHAR, "experiment.platform" VARCHAR, "experiment.instrument_model" VARCHAR, "experiment.spot_descriptor" VARCHAR, "experiment.study_ref" VARCHAR, "run.title" VARCHAR, "run.attributes" VARCHAR, "run.filename" VARCHAR, "run.semantic_name" VARCHAR, "run.total_bases" DOUBLE, "run.total_spots" DOUBLE, "run.alias" VARCHAR, "run.read_lengths" VARCHAR, "run.base_counts" VARCHAR, "run.r1_length" BIGINT, "run.r2_length" BIGINT, "run.r3_length" BIGINT, "run.r4_length" BIGINT, "run.Acount" BIGINT, "run.Ccount" BIGINT, "run.Gcount" BIGINT, "run.Tcount" BIGINT, "run.Ncount" BIGINT, "run.experiment" VARCHAR, "run.pool_member" VARCHAR, "submission.accession" VARCHAR, "submission.srasource" VARCHAR, "submission.bioprojectsource" VARCHAR, "seqdetective.n_mates" BIGINT, "seqdetective.mapping_rate.mate1" DOUBLE, "seqdetective.mapping_rate.mate2" DOUBLE, "seqdetective.nofeature_rate.mate1" DOUBLE, "seqdetective.nofeature_rate.mate2" DOUBLE, "seqdetective.sparsity.mate1" DOUBLE, "seqdetective.sparsity.mate2" DOUBLE, "seqdetective.pos_strand_rate.mate1" DOUBLE, "seqdetective.pos_strand_rate.mate2" DOUBLE, "seqdetective.readlen.mate1" BIGINT, "seqdetective.readlen.mate2" BIGINT, "seqdetective.judgement.mate1" VARCHAR, "seqdetective.judgement.mate2" VARCHAR, "seqdetective.judgement.reason" VARCHAR, platform_family VARCHAR, instrument_generation VARCHAR, read_bias VARCHAR, selection_class VARCHAR, prep_kit VARCHAR, sc_or_bulk VARCHAR, tech_class VARCHAR, technology VARCHAR, tech_variant VARCHAR, "submission.bioprojectsource.country" VARCHAR, earliest_date DATE, devstage_curation VARCHAR, devstage_curation_coarse VARCHAR, tissue_curation VARCHAR, tissue_curation_coarse VARCHAR);;
CREATE INDEX idx_run_bioproject ON run_metadata(bioproject);;
CREATE INDEX idx_run_run_accession ON run_metadata("run.accession");;