run_metadata
32 rows where experiment.library_selection = "PCR", experiment.library_source = "TRANSCRIPTOMIC" and tissue_curation_coarse = "Undetermined"
This data as json, CSV (advanced)
| Link | rowid ▼ | run.accession | experiment.accession | sample.accession | study.accession | bioproject | study.title | study.alias | study.type | study.abstract | study.attributes | study.PMIDs | sample.description | sample.title | sample.alias | sample.centername | sample.attributes | GEOsample.title | GEOsample.dataprocessing | GEOsample.source | GEOsample.treatmentprotocol | GEOsample.extractprotocol | GEOsample.growthprotocol | GEOsample.characteristics | GEOsample.accession | experiment.title | experiment.alias | experiment.library_name | experiment.design_description | experiment.library_construction_protocol | experiment.attributes | experiment.library_strategy | experiment.library_source | experiment.library_selection | experiment.library_layout | experiment.platform | experiment.instrument_model | experiment.spot_descriptor | experiment.study_ref | run.title | run.attributes | run.filename | run.semantic_name | run.total_bases | run.total_spots | run.alias | run.read_lengths | run.base_counts | run.r1_length | run.r2_length | run.r3_length | run.r4_length | run.Acount | run.Ccount | run.Gcount | run.Tcount | run.Ncount | run.experiment | run.pool_member | submission.accession | submission.srasource | submission.bioprojectsource | seqdetective.n_mates | seqdetective.mapping_rate.mate1 | seqdetective.mapping_rate.mate2 | seqdetective.nofeature_rate.mate1 | seqdetective.nofeature_rate.mate2 | seqdetective.sparsity.mate1 | seqdetective.sparsity.mate2 | seqdetective.pos_strand_rate.mate1 | seqdetective.pos_strand_rate.mate2 | seqdetective.readlen.mate1 | seqdetective.readlen.mate2 | seqdetective.judgement.mate1 | seqdetective.judgement.mate2 | seqdetective.judgement.reason | platform_family | instrument_generation | read_bias | selection_class | prep_kit | sc_or_bulk | tech_class | technology | tech_variant | submission.bioprojectsource.country | earliest_date | devstage_curation | devstage_curation_coarse | tissue_curation | tissue_curation_coarse |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 30250 | 30250 | SRR27756860 | SRX23421865 | SRS20276790 | SRP486416 | PRJNA1069604 | Danio rerio strain:TL | breed:Danio rerio Raw sequence reads | PRJNA1069604 | Other | To study the effect of a gene deletion in zebrafish | Model organism or animal sample from danio rerio | cobll1a | ecotype:missing|dev stage:missing|collection date:2023 02 21|geo loc name:missing|sex:neuter|tissue:FISH|biomaterial provider:missing|z:1000|BioSampleModel:Model organism or animal | Sample A | 1 | 1 | common method | RNA-Seq | TRANSCRIPTOMIC | PCR | PAIRED | ILLUMINA | HiSeq X Ten | SRP486416 | cobll1a1.R2.fq.gz cobll1a1.R1.fq.gz | fastq fastq | 7119566009.0 | 24893517.0 | cobll1a1.R1.fq.gz | 0:143.02 1:142.98 | A:1899718926;C:1652762152;G:1669973661;T:1897092278;N:18992 | 143 | 142 | 1899718926 | 1652762152 | 1669973661 | 1897092278 | 18992 | SRX23421865 | SRS20276790 | SRA1792726 | Hunan Normal University|Hunan Normal University | Hunan Normal University Hunan Normal University Hunan Normal University | 2 | 0.95825 | 0.95956 | 0.0788 | 0.07795 | 0.67894 | 0.67884 | 0.48342 | 0.48268 | 150 | 150 | B | B | biological fallback assumption | illumina | hiseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | China | 2024-01-29 | Undetermined | Undetermined | Undetermined | Undetermined | ||||||||||||||||||||
| 30251 | 30251 | SRR27756861 | SRX23421864 | SRS20276789 | SRP486416 | PRJNA1069604 | Danio rerio strain:TL | breed:Danio rerio Raw sequence reads | PRJNA1069604 | Other | To study the effect of a gene deletion in zebrafish | MIMARKS Specimen sample from Danio rerio | WT | strain:WT|collection date:2023 02 28|depth:missing|elev:missing|env broad scale:missing|env local scale:missing|env medium:missing|geo loc name:missing|isol growth condt:missing|lat lon:missing|BioSampleModel:MIMARKS.specimen|BioSampleModel:MIGS/MIMS/MIMARKS.microbial | Sample L | 11 | 11 | common method | RNA-Seq | TRANSCRIPTOMIC | PCR | PAIRED | ILLUMINA | HiSeq X Ten | SRP486416 | tu3.R1.fq.gz tu3.R2.fq.gz | fastq fastq | 6881670461.0 | 24208326.0 | tu3.R1.fq.gz | 0:142.15 1:142.12 | A:1820714788;C:1612866668;G:1629078247;T:1818992861;N:17897 | 142 | 142 | 1820714788 | 1612866668 | 1629078247 | 1818992861 | 17897 | SRX23421864 | SRS20276789 | SRA1792726 | Hunan Normal University|Hunan Normal University | Hunan Normal University Hunan Normal University Hunan Normal University | 2 | 0.95713 | 0.95821 | 0.07191 | 0.07071 | 0.67801 | 0.67726 | 0.48591 | 0.48501 | 150 | 150 | B | B | biological fallback assumption | illumina | hiseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | China | 2024-01-29 | Undetermined | Undetermined | Undetermined | Undetermined | ||||||||||||||||||||
| 30252 | 30252 | SRR27756862 | SRX23421863 | SRS20276790 | SRP486416 | PRJNA1069604 | Danio rerio strain:TL | breed:Danio rerio Raw sequence reads | PRJNA1069604 | Other | To study the effect of a gene deletion in zebrafish | Model organism or animal sample from danio rerio | cobll1a | ecotype:missing|dev stage:missing|collection date:2023 02 21|geo loc name:missing|sex:neuter|tissue:FISH|biomaterial provider:missing|z:1000|BioSampleModel:Model organism or animal | Sample C | 3 | 3 | common method | RNA-Seq | TRANSCRIPTOMIC | PCR | PAIRED | ILLUMINA | HiSeq X Ten | SRP486416 | cobll1a2.R1.fq.gz cobll1a2.R2.fq.gz | fastq fastq | 6639076778.0 | 23213579.0 | cobll1a2.R1.fq.gz | 0:143.02 1:142.98 | A:1799749408;C:1513051586;G:1530064901;T:1796193421;N:17462 | 143 | 142 | 1799749408 | 1513051586 | 1530064901 | 1796193421 | 17462 | SRX23421863 | SRS20276790 | SRA1792726 | Hunan Normal University|Hunan Normal University | Hunan Normal University Hunan Normal University Hunan Normal University | 2 | 0.95695 | 0.95781 | 0.091 | 0.0896 | 0.68199 | 0.68075 | 0.49051 | 0.49057 | 150 | 150 | B | B | biological fallback assumption | illumina | hiseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | China | 2024-01-29 | Undetermined | Undetermined | Undetermined | Undetermined | ||||||||||||||||||||
| 30253 | 30253 | SRR27756863 | SRX23421862 | SRS20276790 | SRP486416 | PRJNA1069604 | Danio rerio strain:TL | breed:Danio rerio Raw sequence reads | PRJNA1069604 | Other | To study the effect of a gene deletion in zebrafish | Model organism or animal sample from danio rerio | cobll1a | ecotype:missing|dev stage:missing|collection date:2023 02 21|geo loc name:missing|sex:neuter|tissue:FISH|biomaterial provider:missing|z:1000|BioSampleModel:Model organism or animal | Sample E | 5 | 5 | common method | RNA-Seq | TRANSCRIPTOMIC | PCR | PAIRED | ILLUMINA | HiSeq X Ten | SRP486416 | cobll1a3.R2.fq.gz cobll1a3.R1.fq.gz | fastq fastq | 6697695084.0 | 23490214.0 | cobll1a3.R1.fq.gz | 0:142.58 1:142.55 | A:1755043056;C:1586242926;G:1602146148;T:1754245480;N:17474 | 142 | 142 | 1755043056 | 1586242926 | 1602146148 | 1754245480 | 17474 | SRX23421862 | SRS20276790 | SRA1792726 | Hunan Normal University|Hunan Normal University | Hunan Normal University Hunan Normal University Hunan Normal University | 2 | 0.96007 | 0.96224 | 0.06487 | 0.06404 | 0.66939 | 0.66906 | 0.48829 | 0.48765 | 130 | 130 | B | B | biological fallback assumption | illumina | hiseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | China | 2024-01-29 | Undetermined | Undetermined | Undetermined | Undetermined | ||||||||||||||||||||
| 30254 | 30254 | SRR27756864 | SRX23421861 | SRS20276789 | SRP486416 | PRJNA1069604 | Danio rerio strain:TL | breed:Danio rerio Raw sequence reads | PRJNA1069604 | Other | To study the effect of a gene deletion in zebrafish | MIMARKS Specimen sample from Danio rerio | WT | strain:WT|collection date:2023 02 28|depth:missing|elev:missing|env broad scale:missing|env local scale:missing|env medium:missing|geo loc name:missing|isol growth condt:missing|lat lon:missing|BioSampleModel:MIMARKS.specimen|BioSampleModel:MIGS/MIMS/MIMARKS.microbial | Sample G | 7 | 7 | common method | RNA-Seq | TRANSCRIPTOMIC | PCR | PAIRED | ILLUMINA | HiSeq X Ten | SRP486416 | tu1.R1.fq.gz tu1.R2.fq.gz | fastq fastq | 7124072376.0 | 25020568.0 | tu1.R1.fq.gz | 0:142.38 1:142.35 | A:1889928651;C:1664950324;G:1681768417;T:1887406325;N:18659 | 142 | 142 | 1889928651 | 1664950324 | 1681768417 | 1887406325 | 18659 | SRX23421861 | SRS20276789 | SRA1792726 | Hunan Normal University|Hunan Normal University | Hunan Normal University Hunan Normal University Hunan Normal University | 2 | 0.96032 | 0.96145 | 0.07398 | 0.07359 | 0.68503 | 0.6843 | 0.48524 | 0.48933 | 116 | 116 | B | B | biological fallback assumption | illumina | hiseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | China | 2024-01-29 | Undetermined | Undetermined | Undetermined | Undetermined | ||||||||||||||||||||
| 30255 | 30255 | SRR27756865 | SRX23421860 | SRS20276789 | SRP486416 | PRJNA1069604 | Danio rerio strain:TL | breed:Danio rerio Raw sequence reads | PRJNA1069604 | Other | To study the effect of a gene deletion in zebrafish | MIMARKS Specimen sample from Danio rerio | WT | strain:WT|collection date:2023 02 28|depth:missing|elev:missing|env broad scale:missing|env local scale:missing|env medium:missing|geo loc name:missing|isol growth condt:missing|lat lon:missing|BioSampleModel:MIMARKS.specimen|BioSampleModel:MIGS/MIMS/MIMARKS.microbial | Sample J | 9 | 9 | common method | RNA-Seq | TRANSCRIPTOMIC | PCR | PAIRED | ILLUMINA | HiSeq X Ten | SRP486416 | tu2.R1.fq.gz tu2.R2.fq.gz | fastq fastq | 7024719799.0 | 24627933.0 | tu2.R1.fq.gz | 0:142.63 1:142.60 | A:1841519314;C:1662861553;G:1679614735;T:1840705226;N:18971 | 142 | 142 | 1841519314 | 1662861553 | 1679614735 | 1840705226 | 18971 | SRX23421860 | SRS20276789 | SRA1792726 | Hunan Normal University|Hunan Normal University | Hunan Normal University Hunan Normal University Hunan Normal University | 2 | 0.96006 | 0.96141 | 0.06713 | 0.06631 | 0.67464 | 0.67426 | 0.48684 | 0.48648 | 150 | 150 | B | B | biological fallback assumption | illumina | hiseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | China | 2024-01-29 | Undetermined | Undetermined | Undetermined | Undetermined | ||||||||||||||||||||
| 34593 | 34593 | SRR32128857 | SRX27475161 | SRS23899211 | SRP559934 | PRJNA1215774 | 3 mpf dark reared zebrafish eye RNA sequencing | PRJNA1215774 | Other | Outdoor time and light intensity are important emerging factors affecting myopia; however the underlying mechanisms remain unknown. To clarify the possible molecular mechanisms underlying myopia caused by dark environment 3 mpf zebrafish eye RNA sequencing was performed. | lighttreated4 | strain:not applicable|isolate:Sun Yat sen University|breed:not applicable10|cultivar:not applicable|ecotype:not applicable|age:not applicable|dev stage:not applicable10|collection date:2021 04 16|geo loc name:China: GuangZhou|sex:not applicable|tissue:zebrafish10|isolation source:zebrafish10|BioSampleModel:Model organism or animal | sample info | library 10 | library 10 | DNA barcode10 | WXS | TRANSCRIPTOMIC | PCR | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP559934 | lighttreated4.read1.fastq.gz lighttreated4.read2.fastq.gz | fastq fastq | 6388100400.0 | 21293668.0 | lighttreated4.read1.fastq.gz | 0:150 1:150 | A:1711345062;C:1474552325;G:1531680528;T:1670485285;N:37200 | 150 | 150 | 1711345062 | 1474552325 | 1531680528 | 1670485285 | 37200 | SRX27475161 | SRS23899211 | SRA2060897 | zhujiang hospital|ophthalmology | zhujiang hospital | B | B | biological fallback assumption | illumina | novaseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | China | 2025-01-25 | Undetermined | Adult | Undetermined | Undetermined | ||||||||||||||||||||||||||||||||
| 34594 | 34594 | SRR32128858 | SRX27475160 | SRS23899210 | SRP559934 | PRJNA1215774 | 3 mpf dark reared zebrafish eye RNA sequencing | PRJNA1215774 | Other | Outdoor time and light intensity are important emerging factors affecting myopia; however the underlying mechanisms remain unknown. To clarify the possible molecular mechanisms underlying myopia caused by dark environment 3 mpf zebrafish eye RNA sequencing was performed. | lighttreated3 | strain:not applicable|isolate:Sun Yat sen University|breed:not applicable9|cultivar:not applicable|ecotype:not applicable|age:not applicable|dev stage:not applicable9|collection date:2021 04 16|geo loc name:China: GuangZhou|sex:not applicable|tissue:zebrafish9|isolation source:zebrafish9|BioSampleModel:Model organism or animal | sample info | library 9 | library 9 | DNA barcode9 | WXS | TRANSCRIPTOMIC | PCR | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP559934 | lighttreated3.read1.fastq.gz lighttreated3.read2.fastq.gz | fastq fastq | 7919383200.0 | 26397944.0 | lighttreated3.read1.fastq.gz | 0:150 1:150 | A:2135212601;C:1813586654;G:1892878979;T:2077658764;N:46202 | 150 | 150 | 2135212601 | 1813586654 | 1892878979 | 2077658764 | 46202 | SRX27475160 | SRS23899210 | SRA2060897 | zhujiang hospital|ophthalmology | zhujiang hospital | B | B | biological fallback assumption | illumina | novaseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | China | 2025-01-25 | Undetermined | Adult | Undetermined | Undetermined | ||||||||||||||||||||||||||||||||
| 34595 | 34595 | SRR32128859 | SRX27475159 | SRS23899209 | SRP559934 | PRJNA1215774 | 3 mpf dark reared zebrafish eye RNA sequencing | PRJNA1215774 | Other | Outdoor time and light intensity are important emerging factors affecting myopia; however the underlying mechanisms remain unknown. To clarify the possible molecular mechanisms underlying myopia caused by dark environment 3 mpf zebrafish eye RNA sequencing was performed. | lighttreated2 | strain:not applicable|isolate:Sun Yat sen University|breed:not applicable8|cultivar:not applicable|ecotype:not applicable|age:not applicable|dev stage:not applicable8|collection date:2021 04 16|geo loc name:China: GuangZhou|sex:not applicable|tissue:zebrafish8|isolation source:zebrafish8|BioSampleModel:Model organism or animal | sample info | library 8 | library 8 | DNA barcode8 | WXS | TRANSCRIPTOMIC | PCR | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP559934 | lighttreated2.read1.fastq.gz lighttreated2.read2.fastq.gz | fastq fastq | 7060994100.0 | 23536647.0 | lighttreated2.read1.fastq.gz | 0:150 1:150 | A:1921099545;C:1595276449;G:1668657714;T:1875918159;N:42233 | 150 | 150 | 1921099545 | 1595276449 | 1668657714 | 1875918159 | 42233 | SRX27475159 | SRS23899209 | SRA2060897 | zhujiang hospital|ophthalmology | zhujiang hospital | B | B | biological fallback assumption | illumina | novaseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | China | 2025-01-25 | Undetermined | Adult | Undetermined | Undetermined | ||||||||||||||||||||||||||||||||
| 34596 | 34596 | SRR32128860 | SRX27475158 | SRS23899208 | SRP559934 | PRJNA1215774 | 3 mpf dark reared zebrafish eye RNA sequencing | PRJNA1215774 | Other | Outdoor time and light intensity are important emerging factors affecting myopia; however the underlying mechanisms remain unknown. To clarify the possible molecular mechanisms underlying myopia caused by dark environment 3 mpf zebrafish eye RNA sequencing was performed. | lighttreated1 | strain:not applicable|isolate:Sun Yat sen University|breed:not applicable7|cultivar:not applicable|ecotype:not applicable|age:not applicable|dev stage:not applicable7|collection date:2021 04 16|geo loc name:China: GuangZhou|sex:not applicable|tissue:zebrafish7|isolation source:zebrafish7|BioSampleModel:Model organism or animal | sample info | library 7 | library 7 | DNA barcode7 | WXS | TRANSCRIPTOMIC | PCR | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP559934 | lighttreated1.read1.fastq.gz lighttreated1.read2.fastq.gz | fastq fastq | 6908625300.0 | 23028751.0 | lighttreated1.read1.fastq.gz | 0:150 1:150 | A:1872190225;C:1565342714;G:1644929113;T:1826124355;N:38893 | 150 | 150 | 1872190225 | 1565342714 | 1644929113 | 1826124355 | 38893 | SRX27475158 | SRS23899208 | SRA2060897 | zhujiang hospital|ophthalmology | zhujiang hospital | B | B | biological fallback assumption | illumina | novaseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | China | 2025-01-25 | Undetermined | Adult | Undetermined | Undetermined | ||||||||||||||||||||||||||||||||
| 34597 | 34597 | SRR32128861 | SRX27475157 | SRS23899207 | SRP559934 | PRJNA1215774 | 3 mpf dark reared zebrafish eye RNA sequencing | PRJNA1215774 | Other | Outdoor time and light intensity are important emerging factors affecting myopia; however the underlying mechanisms remain unknown. To clarify the possible molecular mechanisms underlying myopia caused by dark environment 3 mpf zebrafish eye RNA sequencing was performed. | darktreated6 | strain:not applicable|isolate:Sun Yat sen University|breed:not applicable6|cultivar:not applicable|ecotype:not applicable|age:not applicable|dev stage:not applicable6|collection date:2021 04 16|geo loc name:China: GuangZhou|sex:not applicable|tissue:zebrafish6|isolation source:zebrafish6|BioSampleModel:Model organism or animal | sample info | library 6 | library 6 | DNA barcode6 | WXS | TRANSCRIPTOMIC | PCR | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP559934 | darktreated6.read1.fastq.gz darktreated6.read2.fastq.gz | fastq fastq | 8601630900.0 | 28672103.0 | darktreated6.read1.fastq.gz | 0:150 1:150 | A:2318530970;C:1973292572;G:2040962638;T:2268796005;N:48715 | 150 | 150 | 2318530970 | 1973292572 | 2040962638 | 2268796005 | 48715 | SRX27475157 | SRS23899207 | SRA2060897 | zhujiang hospital|ophthalmology | zhujiang hospital | B | B | biological fallback assumption | illumina | novaseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | China | 2025-01-25 | Undetermined | Adult | Undetermined | Undetermined | ||||||||||||||||||||||||||||||||
| 34598 | 34598 | SRR32128862 | SRX27475156 | SRS23899206 | SRP559934 | PRJNA1215774 | 3 mpf dark reared zebrafish eye RNA sequencing | PRJNA1215774 | Other | Outdoor time and light intensity are important emerging factors affecting myopia; however the underlying mechanisms remain unknown. To clarify the possible molecular mechanisms underlying myopia caused by dark environment 3 mpf zebrafish eye RNA sequencing was performed. | darktreated5 | strain:not applicable|isolate:Sun Yat sen University|breed:not applicable5|cultivar:not applicable|ecotype:not applicable|age:not applicable|dev stage:not applicable5|collection date:2021 04 16|geo loc name:China: GuangZhou|sex:not applicable|tissue:zebrafish5|isolation source:zebrafish5|BioSampleModel:Model organism or animal | sample info | library 5 | library 5 | DNA barcode5 | WXS | TRANSCRIPTOMIC | PCR | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP559934 | darktreated5.read1.fastq.gz darktreated5.read2.fastq.gz | fastq fastq | 8265255300.0 | 27550851.0 | darktreated5.read1.fastq.gz | 0:150 1:150 | A:2224219375;C:1898065838;G:1969603341;T:2173318912;N:47834 | 150 | 150 | 2224219375 | 1898065838 | 1969603341 | 2173318912 | 47834 | SRX27475156 | SRS23899206 | SRA2060897 | zhujiang hospital|ophthalmology | zhujiang hospital | B | B | biological fallback assumption | illumina | novaseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | China | 2025-01-25 | Undetermined | Adult | Undetermined | Undetermined | ||||||||||||||||||||||||||||||||
| 34599 | 34599 | SRR32128863 | SRX27475155 | SRS23899205 | SRP559934 | PRJNA1215774 | 3 mpf dark reared zebrafish eye RNA sequencing | PRJNA1215774 | Other | Outdoor time and light intensity are important emerging factors affecting myopia; however the underlying mechanisms remain unknown. To clarify the possible molecular mechanisms underlying myopia caused by dark environment 3 mpf zebrafish eye RNA sequencing was performed. | darktreated4 | strain:not applicable|isolate:Sun Yat sen University|breed:not applicable4|cultivar:not applicable|ecotype:not applicable|age:not applicable|dev stage:not applicable4|collection date:2021 04 16|geo loc name:China: GuangZhou|sex:not applicable|tissue:zebrafish4|isolation source:zebrafish4|BioSampleModel:Model organism or animal | sample info | library 4 | library 4 | DNA barcode4 | WXS | TRANSCRIPTOMIC | PCR | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP559934 | darktreated4.read1.fastq.gz darktreated4.read2.fastq.gz | fastq fastq | 8061663600.0 | 26872212.0 | darktreated4.read1.fastq.gz | 0:150 1:150 | A:2171664867;C:1853256385;G:1909447014;T:2127248788;N:46546 | 150 | 150 | 2171664867 | 1853256385 | 1909447014 | 2127248788 | 46546 | SRX27475155 | SRS23899205 | SRA2060897 | zhujiang hospital|ophthalmology | zhujiang hospital | B | B | biological fallback assumption | illumina | novaseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | China | 2025-01-25 | Undetermined | Adult | Undetermined | Undetermined | ||||||||||||||||||||||||||||||||
| 34600 | 34600 | SRR32128864 | SRX27475154 | SRS23899204 | SRP559934 | PRJNA1215774 | 3 mpf dark reared zebrafish eye RNA sequencing | PRJNA1215774 | Other | Outdoor time and light intensity are important emerging factors affecting myopia; however the underlying mechanisms remain unknown. To clarify the possible molecular mechanisms underlying myopia caused by dark environment 3 mpf zebrafish eye RNA sequencing was performed. | darktreated3 | strain:not applicable|isolate:Sun Yat sen University|breed:not applicable3|cultivar:not applicable|ecotype:not applicable|age:not applicable|dev stage:not applicable3|collection date:2021 04 16|geo loc name:China: GuangZhou|sex:not applicable|tissue:zebrafish3|isolation source:zebrafish3|BioSampleModel:Model organism or animal | sample info | library 3 | library 3 | DNA barcode3 | WXS | TRANSCRIPTOMIC | PCR | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP559934 | darktreated3.read1.fastq.gz darktreated3.read2.fastq.gz | fastq fastq | 8287650300.0 | 27625501.0 | darktreated3.read1.fastq.gz | 0:150 1:150 | A:2231337901;C:1902488349;G:1971440974;T:2182335741;N:47335 | 150 | 150 | 2231337901 | 1902488349 | 1971440974 | 2182335741 | 47335 | SRX27475154 | SRS23899204 | SRA2060897 | zhujiang hospital|ophthalmology | zhujiang hospital | B | B | biological fallback assumption | illumina | novaseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | China | 2025-01-25 | Undetermined | Adult | Undetermined | Undetermined | ||||||||||||||||||||||||||||||||
| 34601 | 34601 | SRR32128865 | SRX27475153 | SRS23899203 | SRP559934 | PRJNA1215774 | 3 mpf dark reared zebrafish eye RNA sequencing | PRJNA1215774 | Other | Outdoor time and light intensity are important emerging factors affecting myopia; however the underlying mechanisms remain unknown. To clarify the possible molecular mechanisms underlying myopia caused by dark environment 3 mpf zebrafish eye RNA sequencing was performed. | lighttreated6 | strain:not applicable|isolate:Sun Yat sen University|breed:not applicable12|cultivar:not applicable|ecotype:not applicable|age:not applicable|dev stage:not applicable12|collection date:2021 04 16|geo loc name:China: GuangZhou|sex:not applicable|tissue:zebrafish12|isolation source:zebrafish12|BioSampleModel:Model organism or animal | sample info | library 12 | library 12 | DNA barcode12 | WXS | TRANSCRIPTOMIC | PCR | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP559934 | lighttreated6.read1.fastq.gz lighttreated6.read2.fastq.gz | fastq fastq | 7610579400.0 | 25368598.0 | lighttreated6.read1.fastq.gz | 0:150 1:150 | A:2063115765;C:1733106391;G:1795336188;T:2018975316;N:45740 | 150 | 150 | 2063115765 | 1733106391 | 1795336188 | 2018975316 | 45740 | SRX27475153 | SRS23899203 | SRA2060897 | zhujiang hospital|ophthalmology | zhujiang hospital | B | B | biological fallback assumption | illumina | novaseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | China | 2025-01-25 | Undetermined | Adult | Undetermined | Undetermined | ||||||||||||||||||||||||||||||||
| 34602 | 34602 | SRR32128866 | SRX27475152 | SRS23899202 | SRP559934 | PRJNA1215774 | 3 mpf dark reared zebrafish eye RNA sequencing | PRJNA1215774 | Other | Outdoor time and light intensity are important emerging factors affecting myopia; however the underlying mechanisms remain unknown. To clarify the possible molecular mechanisms underlying myopia caused by dark environment 3 mpf zebrafish eye RNA sequencing was performed. | lighttreated5 | strain:not applicable|isolate:Sun Yat sen University|breed:not applicable11|cultivar:not applicable|ecotype:not applicable|age:not applicable|dev stage:not applicable11|collection date:2021 04 16|geo loc name:China: GuangZhou|sex:not applicable|tissue:zebrafish11|isolation source:zebrafish11|BioSampleModel:Model organism or animal | sample info | library 11 | library 11 | DNA barcode11 | WXS | TRANSCRIPTOMIC | PCR | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP559934 | lighttreated5.read1.fastq.gz lighttreated5.read2.fastq.gz | fastq fastq | 6677234100.0 | 22257447.0 | lighttreated5.read1.fastq.gz | 0:150 1:150 | A:1812295746;C:1506030273;G:1585674870;T:1773195250;N:37961 | 150 | 150 | 1812295746 | 1506030273 | 1585674870 | 1773195250 | 37961 | SRX27475152 | SRS23899202 | SRA2060897 | zhujiang hospital|ophthalmology | zhujiang hospital | B | B | biological fallback assumption | illumina | novaseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | China | 2025-01-25 | Undetermined | Adult | Undetermined | Undetermined | ||||||||||||||||||||||||||||||||
| 34603 | 34603 | SRR32128867 | SRX27475151 | SRS23899201 | SRP559934 | PRJNA1215774 | 3 mpf dark reared zebrafish eye RNA sequencing | PRJNA1215774 | Other | Outdoor time and light intensity are important emerging factors affecting myopia; however the underlying mechanisms remain unknown. To clarify the possible molecular mechanisms underlying myopia caused by dark environment 3 mpf zebrafish eye RNA sequencing was performed. | darktreated2 | strain:not applicable|isolate:Sun Yat sen University|breed:not applicable2|cultivar:not applicable|ecotype:not applicable|age:not applicable|dev stage:not applicable2|collection date:2021 04 16|geo loc name:China: GuangZhou|sex:not applicable|tissue:zebrafish2|isolation source:zebrafish2|BioSampleModel:Model organism or animal | sample info | library 2 | library 2 | DNA barcode2 | WXS | TRANSCRIPTOMIC | PCR | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP559934 | darktreated2.read1.fastq.gz darktreated2.read2.fastq.gz | fastq fastq | 8723972700.0 | 29079909.0 | darktreated2.read1.fastq.gz | 0:150 1:150 | A:2341340036;C:2009282478;G:2082163796;T:2291135873;N:50517 | 150 | 150 | 2341340036 | 2009282478 | 2082163796 | 2291135873 | 50517 | SRX27475151 | SRS23899201 | SRA2060897 | zhujiang hospital|ophthalmology | zhujiang hospital | B | B | biological fallback assumption | illumina | novaseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | China | 2025-01-25 | Undetermined | Adult | Undetermined | Undetermined | ||||||||||||||||||||||||||||||||
| 34604 | 34604 | SRR32128868 | SRX27475150 | SRS23899200 | SRP559934 | PRJNA1215774 | 3 mpf dark reared zebrafish eye RNA sequencing | PRJNA1215774 | Other | Outdoor time and light intensity are important emerging factors affecting myopia; however the underlying mechanisms remain unknown. To clarify the possible molecular mechanisms underlying myopia caused by dark environment 3 mpf zebrafish eye RNA sequencing was performed. | darktreated1 | strain:not applicable|isolate:Sun Yat sen University|breed:not applicable1|cultivar:not applicable|ecotype:not applicable|age:not applicable|dev stage:not applicable1|collection date:2021 04 16|geo loc name:China: GuangZhou|sex:not applicable|tissue:zebrafish1|isolation source:zebrafish1|BioSampleModel:Model organism or animal | sample info | library 1 | library 1 | DNA barcode1 | WXS | TRANSCRIPTOMIC | PCR | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP559934 | darktreated1.read1.fastq.gz darktreated1.read2.fastq.gz | fastq fastq | 7940928300.0 | 26469761.0 | darktreated1.read1.fastq.gz | 0:150 1:150 | A:2133717650;C:1824526059;G:1895330033;T:2087310578;N:43980 | 150 | 150 | 2133717650 | 1824526059 | 1895330033 | 2087310578 | 43980 | SRX27475150 | SRS23899200 | SRA2060897 | zhujiang hospital|ophthalmology | zhujiang hospital | B | B | biological fallback assumption | illumina | novaseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | China | 2025-01-25 | Undetermined | Adult | Undetermined | Undetermined | ||||||||||||||||||||||||||||||||
| 66086 | 66086 | SRR15900240 | SRX12190888 | SRS10168725 | SRP337081 | PRJNA763243 | m6A RNA methylation regulated by AHR contributes to the cardiac developmental toxicity of PM2.5 in zebrafish embryos | PRJNA763243 | Other | This study aims to investigate the roles of m6A RNA methylation regulated by AHR in the heart developmental toxicity of PM2.5 in zebrafish embryos.EOM downregulates the expression levels of methyltransferase METTL14 which can be restored by AHR inhibitor CH or AHR knockdown. In addition EOM increased ROS levels and cell apoptosis in the heart region of zebrafish embryos and these can be attenuated by enforced METTL14 overexpression. In conclusion AHR activated by PM2.5 significantly inhibits METTL14 expression which in turn disrupts heart development via oxidative stress and apoptosis resulting in heart defects in zebrafish embryos. | EOM+CH 2 | strain:AB|isolate:CH2|breed:missing|cultivar:missing|ecotype:missing|age:missing|dev stage:72 hpf organism or animal | RNA m6A methylation of zebrafish | tchenlab CH2 | tchenlab CH2 | M6A RNA immunoprecipitation was performed using GenSeqTM m6A RNA IP kit GenSeq.NEBNextUltra II Directional RNA Library Prep kit was used to construct RNA sequencing libraries for Input RNA samples without xxx and IP RNA samples post immunoprecipitation.Library inspection is performed using the BioAnalyzer 2100 analyzer Agilent.High throughput sequencing was performed using a 150 bp dual terminal mode on Illumina NovaSeq 6000 sequencer. | RIP-Seq | TRANSCRIPTOMIC | PCR | SINGLE | ILLUMINA | Illumina NovaSeq 6000 | SRP337081 | loader:fastq load.py | EOM_CH_5.8.IP_R1.fastq.gz EOM_CH_5.8.IP_R2.fastq.gz EOM_CH_5.8.Input_R1.fastq.gz EOM_CH_5.8.Input_R2.fastq.gz | fastq fastq fastq fastq | 13391108400.0 | 44637028.0 | EOM CH 5.8.IP R1.fastq.gz | 0:150 1:150 | A:2721241817;C:3886076819;G:4096587749;T:2611313908;N:75888107 | 150 | 150 | 2721241817 | 3886076819 | 4096587749 | 2611313908 | 75888107 | SRX12190888 | SRS10168725 | SRA1294636 | Soochow University|Department of Toxicology, School of Public Health | Soochow University | 2 | 0.8442 | 0.59467 | 0.17491 | 0.14724 | 0.86147 | 0.89112 | 0.7593 | 0.63363 | 150 | 150 | B | B | mate2-mate1 similar by mapping diff | illumina | novaseq_era | unknown | random_priming | nebnext | bulk | unknown | unknown | Unknown | 2021-09-15 | Larval | Larval | Undetermined | Undetermined | ||||||||||||||||||||
| 66087 | 66087 | SRR15900241 | SRX12190887 | SRS10168724 | SRP337081 | PRJNA763243 | m6A RNA methylation regulated by AHR contributes to the cardiac developmental toxicity of PM2.5 in zebrafish embryos | PRJNA763243 | Other | This study aims to investigate the roles of m6A RNA methylation regulated by AHR in the heart developmental toxicity of PM2.5 in zebrafish embryos.EOM downregulates the expression levels of methyltransferase METTL14 which can be restored by AHR inhibitor CH or AHR knockdown. In addition EOM increased ROS levels and cell apoptosis in the heart region of zebrafish embryos and these can be attenuated by enforced METTL14 overexpression. In conclusion AHR activated by PM2.5 significantly inhibits METTL14 expression which in turn disrupts heart development via oxidative stress and apoptosis resulting in heart defects in zebrafish embryos. | EOM+CH 1 | strain:AB|isolate:CH1|breed:missing|cultivar:missing|ecotype:missing|age:missing|dev stage:72 hpf organism or animal | RNA m6A methylation of zebrafish | tchenlab CH1 | tchenlab CH1 | M6A RNA immunoprecipitation was performed using GenSeqTM m6A RNA IP kit GenSeq.NEBNextUltra II Directional RNA Library Prep kit was used to construct RNA sequencing libraries for Input RNA samples without xxx and IP RNA samples post immunoprecipitation.Library inspection is performed using the BioAnalyzer 2100 analyzer Agilent.High throughput sequencing was performed using a 150 bp dual terminal mode on Illumina NovaSeq 6000 sequencer. | RIP-Seq | TRANSCRIPTOMIC | PCR | SINGLE | ILLUMINA | Illumina NovaSeq 6000 | SRP337081 | loader:fastq load.py | EOM_CH_4.16.IP_R1.fastq.gz EOM_CH_4.16.IP_R2.fastq.gz EOM_CH_4.16.Input_R1.fastq.gz EOM_CH_4.16.Input_R2.fastq.gz | fastq fastq fastq fastq | 12428976000.0 | 41429920.0 | EOM CH 4.16.IP R1.fastq.gz | 0:150 1:150 | A:2486344612;C:3648199763;G:3865825828;T:2365039093;N:63566704 | 150 | 150 | 2486344612 | 3648199763 | 3865825828 | 2365039093 | 63566704 | SRX12190887 | SRS10168724 | SRA1294636 | Soochow University|Department of Toxicology, School of Public Health | Soochow University | 2 | 0.91763 | 0.6223 | 0.18817 | 0.15102 | 0.84891 | 0.87957 | 0.74757 | 0.64429 | 150 | 150 | B | B | mate2-mate1 similar by mapping diff | illumina | novaseq_era | unknown | random_priming | nebnext | bulk | unknown | unknown | Unknown | 2021-09-15 | Larval | Larval | Undetermined | Undetermined | ||||||||||||||||||||
| 66088 | 66088 | SRR15900242 | SRX12190886 | SRS10168723 | SRP337081 | PRJNA763243 | m6A RNA methylation regulated by AHR contributes to the cardiac developmental toxicity of PM2.5 in zebrafish embryos | PRJNA763243 | Other | This study aims to investigate the roles of m6A RNA methylation regulated by AHR in the heart developmental toxicity of PM2.5 in zebrafish embryos.EOM downregulates the expression levels of methyltransferase METTL14 which can be restored by AHR inhibitor CH or AHR knockdown. In addition EOM increased ROS levels and cell apoptosis in the heart region of zebrafish embryos and these can be attenuated by enforced METTL14 overexpression. In conclusion AHR activated by PM2.5 significantly inhibits METTL14 expression which in turn disrupts heart development via oxidative stress and apoptosis resulting in heart defects in zebrafish embryos. | EOM 3 | strain:AB|isolate:T3|breed:missing|cultivar:missing|ecotype:missing|age:missing|dev stage:72 hpf organism or animal | RNA m6A methylation of zebrafish | tchenlab T3 | tchenlab T3 | M6A RNA immunoprecipitation was performed using GenSeqTM m6A RNA IP kit GenSeq.NEBNextUltra II Directional RNA Library Prep kit was used to construct RNA sequencing libraries for Input RNA samples without xxx and IP RNA samples post immunoprecipitation.Library inspection is performed using the BioAnalyzer 2100 analyzer Agilent.High throughput sequencing was performed using a 150 bp dual terminal mode on Illumina NovaSeq 6000 sequencer. | RIP-Seq | TRANSCRIPTOMIC | PCR | SINGLE | ILLUMINA | Illumina NovaSeq 6000 | SRP337081 | loader:fastq load.py | EOM_5.9.IP_R1.fastq.gz EOM_5.9.IP_R2.fastq.gz EOM_5.9.Input_R1.fastq.gz EOM_5.9.Input_R2.fastq.gz | fastq fastq fastq fastq | 12301780200.0 | 41005934.0 | EOM 5.9.IP R1.fastq.gz | 0:150 1:150 | A:2415542475;C:3651603114;G:3847935468;T:2318868176;N:67830967 | 150 | 150 | 2415542475 | 3651603114 | 3847935468 | 2318868176 | 67830967 | SRX12190886 | SRS10168723 | SRA1294636 | Soochow University|Department of Toxicology, School of Public Health | Soochow University | 2 | 0.87516 | 0.65874 | 0.18282 | 0.16547 | 0.85561 | 0.87657 | 0.72193 | 0.61862 | 150 | 150 | B | B | biological fallback assumption | illumina | novaseq_era | unknown | random_priming | nebnext | bulk | unknown | unknown | Unknown | 2021-09-15 | Larval | Larval | Undetermined | Undetermined | ||||||||||||||||||||
| 66089 | 66089 | SRR15900243 | SRX12190885 | SRS10168722 | SRP337081 | PRJNA763243 | m6A RNA methylation regulated by AHR contributes to the cardiac developmental toxicity of PM2.5 in zebrafish embryos | PRJNA763243 | Other | This study aims to investigate the roles of m6A RNA methylation regulated by AHR in the heart developmental toxicity of PM2.5 in zebrafish embryos.EOM downregulates the expression levels of methyltransferase METTL14 which can be restored by AHR inhibitor CH or AHR knockdown. In addition EOM increased ROS levels and cell apoptosis in the heart region of zebrafish embryos and these can be attenuated by enforced METTL14 overexpression. In conclusion AHR activated by PM2.5 significantly inhibits METTL14 expression which in turn disrupts heart development via oxidative stress and apoptosis resulting in heart defects in zebrafish embryos. | EOM 2 | strain:AB|isolate:T2|breed:missing|cultivar:missing|ecotype:missing|age:missing|dev stage:72 hpf organism or animal | RNA m6A methylation of zebrafish | tchenlab T2 | tchenlab T2 | M6A RNA immunoprecipitation was performed using GenSeqTM m6A RNA IP kit GenSeq.NEBNextUltra II Directional RNA Library Prep kit was used to construct RNA sequencing libraries for Input RNA samples without xxx and IP RNA samples post immunoprecipitation.Library inspection is performed using the BioAnalyzer 2100 analyzer Agilent.High throughput sequencing was performed using a 150 bp dual terminal mode on Illumina NovaSeq 6000 sequencer. | RIP-Seq | TRANSCRIPTOMIC | PCR | SINGLE | ILLUMINA | Illumina NovaSeq 6000 | SRP337081 | loader:fastq load.py | EOM_5.8.IP_R1.fastq.gz EOM_5.8.IP_R2.fastq.gz EOM_5.8.Input_R1.fastq.gz EOM_5.8.Input_R2.fastq.gz | fastq fastq fastq fastq | 12373139700.0 | 41243799.0 | EOM 5.8.IP R1.fastq.gz | 0:150 1:150 | A:2429212483;C:3679585650;G:3865741937;T:2330164654;N:68434976 | 150 | 150 | 2429212483 | 3679585650 | 3865741937 | 2330164654 | 68434976 | SRX12190885 | SRS10168722 | SRA1294636 | Soochow University|Department of Toxicology, School of Public Health | Soochow University | 2 | 0.88644 | 0.67829 | 0.18408 | 0.16867 | 0.84652 | 0.8704 | 0.76376 | 0.64158 | 150 | 150 | B | B | biological fallback assumption | illumina | novaseq_era | unknown | random_priming | nebnext | bulk | unknown | unknown | Unknown | 2021-09-15 | Larval | Larval | Undetermined | Undetermined | ||||||||||||||||||||
| 66090 | 66090 | SRR15900244 | SRX12190884 | SRS10168721 | SRP337081 | PRJNA763243 | m6A RNA methylation regulated by AHR contributes to the cardiac developmental toxicity of PM2.5 in zebrafish embryos | PRJNA763243 | Other | This study aims to investigate the roles of m6A RNA methylation regulated by AHR in the heart developmental toxicity of PM2.5 in zebrafish embryos.EOM downregulates the expression levels of methyltransferase METTL14 which can be restored by AHR inhibitor CH or AHR knockdown. In addition EOM increased ROS levels and cell apoptosis in the heart region of zebrafish embryos and these can be attenuated by enforced METTL14 overexpression. In conclusion AHR activated by PM2.5 significantly inhibits METTL14 expression which in turn disrupts heart development via oxidative stress and apoptosis resulting in heart defects in zebrafish embryos. | EOM 1 | strain:AB|isolate:T1|breed:missing|cultivar:missing|ecotype:missing|age:missing|dev stage:72 hpf organism or animal | RNA m6A methylation of zebrafish | tchenlab T1 | tchenlab T1 | M6A RNA immunoprecipitation was performed using GenSeqTM m6A RNA IP kit GenSeq.NEBNextUltra II Directional RNA Library Prep kit was used to construct RNA sequencing libraries for Input RNA samples without xxx and IP RNA samples post immunoprecipitation.Library inspection is performed using the BioAnalyzer 2100 analyzer Agilent.High throughput sequencing was performed using a 150 bp dual terminal mode on Illumina NovaSeq 6000 sequencer. | RIP-Seq | TRANSCRIPTOMIC | PCR | SINGLE | ILLUMINA | Illumina NovaSeq 6000 | SRP337081 | loader:fastq load.py | EOM_4.16.IP_R1.fastq.gz EOM_4.16.IP_R2.fastq.gz EOM_4.16.Input_R1.fastq.gz EOM_4.16.Input_R2.fastq.gz | fastq fastq fastq fastq | 13638730800.0 | 45462436.0 | EOM 4.16.IP R1.fastq.gz | 0:150 1:150 | A:2673949221;C:4057121296;G:4266457677;T:2562131972;N:79070634 | 150 | 150 | 2673949221 | 4057121296 | 4266457677 | 2562131972 | 79070634 | SRX12190884 | SRS10168721 | SRA1294636 | Soochow University|Department of Toxicology, School of Public Health | Soochow University | 2 | 0.87634 | 0.65649 | 0.18403 | 0.16375 | 0.85486 | 0.87913 | 0.69253 | 0.6405 | 150 | 150 | B | B | mate2-mate1 similar by mapping diff | illumina | novaseq_era | unknown | random_priming | nebnext | bulk | unknown | unknown | Unknown | 2021-09-15 | Larval | Larval | Undetermined | Undetermined | ||||||||||||||||||||
| 66091 | 66091 | SRR15900245 | SRX12190883 | SRS10168720 | SRP337081 | PRJNA763243 | m6A RNA methylation regulated by AHR contributes to the cardiac developmental toxicity of PM2.5 in zebrafish embryos | PRJNA763243 | Other | This study aims to investigate the roles of m6A RNA methylation regulated by AHR in the heart developmental toxicity of PM2.5 in zebrafish embryos.EOM downregulates the expression levels of methyltransferase METTL14 which can be restored by AHR inhibitor CH or AHR knockdown. In addition EOM increased ROS levels and cell apoptosis in the heart region of zebrafish embryos and these can be attenuated by enforced METTL14 overexpression. In conclusion AHR activated by PM2.5 significantly inhibits METTL14 expression which in turn disrupts heart development via oxidative stress and apoptosis resulting in heart defects in zebrafish embryos. | DMSO 3 | strain:AB|isolate:C3|breed:missing|cultivar:missing|ecotype:missing|age:missing|dev stage:72 hpf organism or animal | RNA m6A methylation of zebrafish | tchenlab C3 | tchenlab C3 | M6A RNA immunoprecipitation was performed using GenSeqTM m6A RNA IP kit GenSeq.NEBNextUltra II Directional RNA Library Prep kit was used to construct RNA sequencing libraries for Input RNA samples without xxx and IP RNA samples post immunoprecipitation.Library inspection is performed using the BioAnalyzer 2100 analyzer Agilent.High throughput sequencing was performed using a 150 bp dual terminal mode on Illumina NovaSeq 6000 sequencer. | RIP-Seq | TRANSCRIPTOMIC | PCR | SINGLE | ILLUMINA | Illumina NovaSeq 6000 | SRP337081 | loader:fastq load.py | DMSO_5.9.IP_R1.fastq.gz DMSO_5.9.IP_R2.fastq.gz DMSO_5.9.Input_R1.fastq.gz DMSO_5.9.Input_R2.fastq.gz | fastq fastq fastq fastq | 12477964200.0 | 41593214.0 | DMSO 5.9.IP R1.fastq.gz | 0:150 1:150 | A:2462053397;C:3697418372;G:3878832850;T:2368512335;N:71147246 | 150 | 150 | 2462053397 | 3697418372 | 3878832850 | 2368512335 | 71147246 | SRX12190883 | SRS10168720 | SRA1294636 | Soochow University|Department of Toxicology, School of Public Health | Soochow University | 2 | 0.94535 | 0.75289 | 0.20051 | 0.18779 | 0.84139 | 0.8646 | 0.74818 | 0.65269 | 150 | 150 | B | B | biological fallback assumption | illumina | novaseq_era | unknown | random_priming | nebnext | bulk | unknown | unknown | Unknown | 2021-09-15 | Larval | Larval | Undetermined | Undetermined | ||||||||||||||||||||
| 66092 | 66092 | SRR15900246 | SRX12190882 | SRS10168719 | SRP337081 | PRJNA763243 | m6A RNA methylation regulated by AHR contributes to the cardiac developmental toxicity of PM2.5 in zebrafish embryos | PRJNA763243 | Other | This study aims to investigate the roles of m6A RNA methylation regulated by AHR in the heart developmental toxicity of PM2.5 in zebrafish embryos.EOM downregulates the expression levels of methyltransferase METTL14 which can be restored by AHR inhibitor CH or AHR knockdown. In addition EOM increased ROS levels and cell apoptosis in the heart region of zebrafish embryos and these can be attenuated by enforced METTL14 overexpression. In conclusion AHR activated by PM2.5 significantly inhibits METTL14 expression which in turn disrupts heart development via oxidative stress and apoptosis resulting in heart defects in zebrafish embryos. | DMSO 2 | strain:AB|isolate:C2|breed:missing|cultivar:missing|ecotype:missing|age:missing|dev stage:72 hpf organism or animal | RNA m6A methylation of zebrafish | tchenlab C2 | tchenlab C2 | M6A RNA immunoprecipitation was performed using GenSeqTM m6A RNA IP kit GenSeq.NEBNextUltra II Directional RNA Library Prep kit was used to construct RNA sequencing libraries for Input RNA samples without xxx and IP RNA samples post immunoprecipitation.Library inspection is performed using the BioAnalyzer 2100 analyzer Agilent.High throughput sequencing was performed using a 150 bp dual terminal mode on Illumina NovaSeq 6000 sequencer. | RIP-Seq | TRANSCRIPTOMIC | PCR | SINGLE | ILLUMINA | Illumina NovaSeq 6000 | SRP337081 | loader:fastq load.py | DMSO_5.8.IP_R1.fastq.gz DMSO_5.8.IP_R2.fastq.gz DMSO_5.8.Input_R1.fastq.gz DMSO_5.8.Input_R2.fastq.gz | fastq fastq fastq fastq | 12272849400.0 | 40909498.0 | DMSO 5.8.IP R1.fastq.gz | 0:150 1:150 | A:2381041881;C:3673513092;G:3850518138;T:2295835815;N:71940474 | 150 | 150 | 2381041881 | 3673513092 | 3850518138 | 2295835815 | 71940474 | SRX12190882 | SRS10168719 | SRA1294636 | Soochow University|Department of Toxicology, School of Public Health | Soochow University | 2 | 0.88711 | 0.71046 | 0.18073 | 0.17436 | 0.84654 | 0.8674 | 0.76218 | 0.63257 | 150 | 150 | B | B | biological fallback assumption | illumina | novaseq_era | unknown | random_priming | nebnext | bulk | unknown | unknown | Unknown | 2021-09-15 | Larval | Larval | Undetermined | Undetermined | ||||||||||||||||||||
| 66093 | 66093 | SRR15900247 | SRX12190881 | SRS10168718 | SRP337081 | PRJNA763243 | m6A RNA methylation regulated by AHR contributes to the cardiac developmental toxicity of PM2.5 in zebrafish embryos | PRJNA763243 | Other | This study aims to investigate the roles of m6A RNA methylation regulated by AHR in the heart developmental toxicity of PM2.5 in zebrafish embryos.EOM downregulates the expression levels of methyltransferase METTL14 which can be restored by AHR inhibitor CH or AHR knockdown. In addition EOM increased ROS levels and cell apoptosis in the heart region of zebrafish embryos and these can be attenuated by enforced METTL14 overexpression. In conclusion AHR activated by PM2.5 significantly inhibits METTL14 expression which in turn disrupts heart development via oxidative stress and apoptosis resulting in heart defects in zebrafish embryos. | DMSO 1 | strain:AB|isolate:C1|breed:missing|cultivar:missing|ecotype:missing|age:missing|dev stage:72 hpf organism or animal | RNA m6A methylation of zebrafish | tchenlab C1 | tchenlab C1 | M6A RNA immunoprecipitation was performed using GenSeqTM m6A RNA IP kit GenSeq.NEBNextUltra II Directional RNA Library Prep kit was used to construct RNA sequencing libraries for Input RNA samples without xxx and IP RNA samples post immunoprecipitation.Library inspection is performed using the BioAnalyzer 2100 analyzer Agilent.High throughput sequencing was performed using a 150 bp dual terminal mode on Illumina NovaSeq 6000 sequencer. | RIP-Seq | TRANSCRIPTOMIC | PCR | SINGLE | ILLUMINA | Illumina NovaSeq 6000 | SRP337081 | loader:fastq load.py | DMSO_4.16.IP_R1.fastq.gz DMSO_4.16.IP_R2.fastq.gz DMSO_4.16.Input_R1.fastq.gz DMSO_4.16.Input_R2.fastq.gz | fastq fastq fastq fastq | 12900597000.0 | 43001990.0 | DMSO 4.16.IP R1.fastq.gz | 0:150 1:150 | A:2517062962;C:3854768074;G:4032734290;T:2426295392;N:69736282 | 150 | 150 | 2517062962 | 3854768074 | 4032734290 | 2426295392 | 69736282 | SRX12190881 | SRS10168718 | SRA1294636 | Soochow University|Department of Toxicology, School of Public Health | Soochow University | 2 | 0.75605 | 0.5734 | 0.16268 | 0.14503 | 0.8714 | 0.89422 | 0.72976 | 0.64046 | 150 | 150 | B | B | biological fallback assumption | illumina | novaseq_era | unknown | random_priming | nebnext | bulk | unknown | unknown | Unknown | 2021-09-15 | Larval | Larval | Undetermined | Undetermined | ||||||||||||||||||||
| 68548 | 68548 | SRR18056674 | SRX14208687 | SRS12030730 | SRP360305 | PRJNA806354 | Danio rerio Raw sequence reads | PRJNA806354 | Whole Genome Sequencing | Danio rerio RNA seq reads | Model organism or animal sample from Danio rerio | sample 001 | breed:AB|age:50dpf|sex:not collected|tissue:b1|BioSampleModel:Model organism or animal | SS3WT6 | 6 | 6 | commen method | RNA-Seq | TRANSCRIPTOMIC | PCR | PAIRED | ILLUMINA | HiSeq X Ten | SRP360305 | SS3WT6.R1.fq.gz SS3WT6.R2.fq.gz | fastq fastq | 7174164397.0 | 25400980.0 | SS3WT6.R1.fq.gz | 0:141.31 1:141.13 | A:1934605412;C:1644763885;G:1664938935;T:1929811138;N:45027 | 141 | 141 | 1934605412 | 1644763885 | 1664938935 | 1929811138 | 45027 | SRX14208687 | SRS12030730 | SRA1373545 | Shanghai Ocean University|College of Fisheries and life | Shanghai Ocean University | 2 | 0.94928 | 0.94948 | 0.09187 | 0.09099 | 0.68765 | 0.68769 | 0.50435 | 0.50363 | 150 | 150 | B | B | biological fallback assumption | illumina | hiseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | China | 2022-02-22 | Juvenile | Juvenile | Undetermined | Undetermined | ||||||||||||||||||||
| 68549 | 68549 | SRR18056675 | SRX14208686 | SRS12030730 | SRP360305 | PRJNA806354 | Danio rerio Raw sequence reads | PRJNA806354 | Whole Genome Sequencing | Danio rerio RNA seq reads | Model organism or animal sample from Danio rerio | sample 001 | breed:AB|age:50dpf|sex:not collected|tissue:b1|BioSampleModel:Model organism or animal | SS3WT5 | 5 | 5 | commen method | RNA-Seq | TRANSCRIPTOMIC | PCR | PAIRED | ILLUMINA | HiSeq X Ten | SRP360305 | SS3WT5.R1.fq.gz SS3WT5.R2.fq.gz | fastq fastq | 7159028081.0 | 25133913.0 | SS3WT5.R1.fq.gz | 0:142.50 1:142.33 | A:1905921436;C:1664337487;G:1683294939;T:1905427825;N:46394 | 142 | 142 | 1905921436 | 1664337487 | 1683294939 | 1905427825 | 46394 | SRX14208686 | SRS12030730 | SRA1373545 | Shanghai Ocean University|College of Fisheries and life | Shanghai Ocean University | 2 | 0.94917 | 0.95027 | 0.09384 | 0.09347 | 0.6594 | 0.65894 | 0.49218 | 0.49202 | 122 | 122 | B | B | biological fallback assumption | illumina | hiseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | China | 2022-02-22 | Juvenile | Juvenile | Undetermined | Undetermined | ||||||||||||||||||||
| 68550 | 68550 | SRR18056676 | SRX14208685 | SRS12030730 | SRP360305 | PRJNA806354 | Danio rerio Raw sequence reads | PRJNA806354 | Whole Genome Sequencing | Danio rerio RNA seq reads | Model organism or animal sample from Danio rerio | sample 001 | breed:AB|age:50dpf|sex:not collected|tissue:b1|BioSampleModel:Model organism or animal | SS3WT4 | 4 | 4 | commen method | RNA-Seq | TRANSCRIPTOMIC | PCR | PAIRED | ILLUMINA | HiSeq X Ten | SRP360305 | SS3WT4.R1.fq.gz SS3WT4.R2.fq.gz | fastq fastq | 6985092696.0 | 24360978.0 | SS3WT4.R1.fq.gz | 0:143.44 1:143.29 | A:1858629258;C:1624045323;G:1642960060;T:1859412787;N:45268 | 143 | 143 | 1858629258 | 1624045323 | 1642960060 | 1859412787 | 45268 | SRX14208685 | SRS12030730 | SRA1373545 | Shanghai Ocean University|College of Fisheries and life | Shanghai Ocean University | 2 | 0.94221 | 0.94208 | 0.1047 | 0.1038 | 0.66231 | 0.66251 | 0.49213 | 0.49128 | 113 | 112 | B | B | biological fallback assumption | illumina | hiseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | China | 2022-02-22 | Juvenile | Juvenile | Undetermined | Undetermined | ||||||||||||||||||||
| 68551 | 68551 | SRR18056677 | SRX14208684 | SRS12030730 | SRP360305 | PRJNA806354 | Danio rerio Raw sequence reads | PRJNA806354 | Whole Genome Sequencing | Danio rerio RNA seq reads | Model organism or animal sample from Danio rerio | sample 001 | breed:AB|age:50dpf|sex:not collected|tissue:b1|BioSampleModel:Model organism or animal | SS3WT3 | 3 | 3 | commen method | RNA-Seq | TRANSCRIPTOMIC | PCR | PAIRED | ILLUMINA | HiSeq X Ten | SRP360305 | SS3WT3.R1.fq.gz SS3WT3.R2.fq.gz | fastq fastq | 6604271936.0 | 23189546.0 | SS3WT3.R1.fq.gz | 0:142.47 1:142.32 | A:1765899330;C:1527534084;G:1543609939;T:1767186572;N:42011 | 142 | 142 | 1765899330 | 1527534084 | 1543609939 | 1767186572 | 42011 | SRX14208684 | SRS12030730 | SRA1373545 | Shanghai Ocean University|College of Fisheries and life | Shanghai Ocean University | 2 | 0.94017 | 0.94088 | 0.10254 | 0.10225 | 0.65163 | 0.65167 | 0.47798 | 0.47707 | 131 | 131 | B | B | biological fallback assumption | illumina | hiseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | China | 2022-02-22 | Juvenile | Juvenile | Undetermined | Undetermined | ||||||||||||||||||||
| 68552 | 68552 | SRR18056678 | SRX14208683 | SRS12030730 | SRP360305 | PRJNA806354 | Danio rerio Raw sequence reads | PRJNA806354 | Whole Genome Sequencing | Danio rerio RNA seq reads | Model organism or animal sample from Danio rerio | sample 001 | breed:AB|age:50dpf|sex:not collected|tissue:b1|BioSampleModel:Model organism or animal | SS3WT2 | 2 | 2 | commen method | RNA-Seq | TRANSCRIPTOMIC | PCR | PAIRED | ILLUMINA | HiSeq X Ten | SRP360305 | SS3WT2.R1.fq.gz SS3WT2.R2.fq.gz | fastq fastq | 6674317073.0 | 23265242.0 | SS3WT2.R1.fq.gz | 0:143.52 1:143.36 | A:1768071045;C:1560492667;G:1576548690;T:1769161220;N:43451 | 143 | 143 | 1768071045 | 1560492667 | 1576548690 | 1769161220 | 43451 | SRX14208683 | SRS12030730 | SRA1373545 | Shanghai Ocean University|College of Fisheries and life | Shanghai Ocean University | 2 | 0.94677 | 0.9473 | 0.08956 | 0.08902 | 0.66559 | 0.66557 | 0.48058 | 0.48136 | 150 | 150 | B | B | biological fallback assumption | illumina | hiseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | China | 2022-02-22 | Juvenile | Juvenile | Undetermined | Undetermined | ||||||||||||||||||||
| 68553 | 68553 | SRR18056679 | SRX14208682 | SRS12030730 | SRP360305 | PRJNA806354 | Danio rerio Raw sequence reads | PRJNA806354 | Whole Genome Sequencing | Danio rerio RNA seq reads | Model organism or animal sample from Danio rerio | sample 001 | breed:AB|age:50dpf|sex:not collected|tissue:b1|BioSampleModel:Model organism or animal | SS3WT1 | 1 | 1 | commen method | RNA-Seq | TRANSCRIPTOMIC | PCR | PAIRED | ILLUMINA | HiSeq X Ten | SRP360305 | SS3WT1.R1.fq.gz SS3WT1.R2.fq.gz | fastq fastq | 6848211264.0 | 23787995.0 | SS3WT1.R1.fq.gz | 0:144.06 1:143.82 | A:1825907736;C:1587014566;G:1607121352;T:1828122105;N:45505 | 144 | 143 | 1825907736 | 1587014566 | 1607121352 | 1828122105 | 45505 | SRX14208682 | SRS12030730 | SRA1373545 | Shanghai Ocean University|College of Fisheries and life | Shanghai Ocean University | 2 | 0.94226 | 0.94255 | 0.09053 | 0.08971 | 0.65807 | 0.65823 | 0.48525 | 0.48427 | 150 | 150 | B | B | biological fallback assumption | illumina | hiseq_era | unknown | random_priming | unknown | bulk | unknown | unknown | China | 2022-02-22 | Juvenile | Juvenile | Undetermined | Undetermined |
Advanced export
JSON shape: default, array, newline-delimited
CREATE TABLE run_metadata("run.accession" VARCHAR, "experiment.accession" VARCHAR, "sample.accession" VARCHAR, "study.accession" VARCHAR, bioproject VARCHAR, "study.title" VARCHAR, "study.alias" VARCHAR, "study.type" VARCHAR, "study.abstract" VARCHAR, "study.attributes" VARCHAR, "study.PMIDs" VARCHAR, "sample.description" VARCHAR, "sample.title" VARCHAR, "sample.alias" VARCHAR, "sample.centername" VARCHAR, "sample.attributes" VARCHAR, "GEOsample.title" VARCHAR, "GEOsample.dataprocessing" VARCHAR, "GEOsample.source" VARCHAR, "GEOsample.treatmentprotocol" VARCHAR, "GEOsample.extractprotocol" VARCHAR, "GEOsample.growthprotocol" VARCHAR, "GEOsample.characteristics" VARCHAR, "GEOsample.accession" VARCHAR, "experiment.title" VARCHAR, "experiment.alias" VARCHAR, "experiment.library_name" VARCHAR, "experiment.design_description" VARCHAR, "experiment.library_construction_protocol" VARCHAR, "experiment.attributes" VARCHAR, "experiment.library_strategy" VARCHAR, "experiment.library_source" VARCHAR, "experiment.library_selection" VARCHAR, "experiment.library_layout" VARCHAR, "experiment.platform" VARCHAR, "experiment.instrument_model" VARCHAR, "experiment.spot_descriptor" VARCHAR, "experiment.study_ref" VARCHAR, "run.title" VARCHAR, "run.attributes" VARCHAR, "run.filename" VARCHAR, "run.semantic_name" VARCHAR, "run.total_bases" DOUBLE, "run.total_spots" DOUBLE, "run.alias" VARCHAR, "run.read_lengths" VARCHAR, "run.base_counts" VARCHAR, "run.r1_length" BIGINT, "run.r2_length" BIGINT, "run.r3_length" BIGINT, "run.r4_length" BIGINT, "run.Acount" BIGINT, "run.Ccount" BIGINT, "run.Gcount" BIGINT, "run.Tcount" BIGINT, "run.Ncount" BIGINT, "run.experiment" VARCHAR, "run.pool_member" VARCHAR, "submission.accession" VARCHAR, "submission.srasource" VARCHAR, "submission.bioprojectsource" VARCHAR, "seqdetective.n_mates" BIGINT, "seqdetective.mapping_rate.mate1" DOUBLE, "seqdetective.mapping_rate.mate2" DOUBLE, "seqdetective.nofeature_rate.mate1" DOUBLE, "seqdetective.nofeature_rate.mate2" DOUBLE, "seqdetective.sparsity.mate1" DOUBLE, "seqdetective.sparsity.mate2" DOUBLE, "seqdetective.pos_strand_rate.mate1" DOUBLE, "seqdetective.pos_strand_rate.mate2" DOUBLE, "seqdetective.readlen.mate1" BIGINT, "seqdetective.readlen.mate2" BIGINT, "seqdetective.judgement.mate1" VARCHAR, "seqdetective.judgement.mate2" VARCHAR, "seqdetective.judgement.reason" VARCHAR, platform_family VARCHAR, instrument_generation VARCHAR, read_bias VARCHAR, selection_class VARCHAR, prep_kit VARCHAR, sc_or_bulk VARCHAR, tech_class VARCHAR, technology VARCHAR, tech_variant VARCHAR, "submission.bioprojectsource.country" VARCHAR, earliest_date DATE, devstage_curation VARCHAR, devstage_curation_coarse VARCHAR, tissue_curation VARCHAR, tissue_curation_coarse VARCHAR);;
CREATE INDEX idx_run_bioproject ON run_metadata(bioproject);;
CREATE INDEX idx_run_run_accession ON run_metadata("run.accession");;