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188 rows where experiment.library_selection = "Oligo-dT" and tissue_curation_coarse = "Reproductive System"

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devstage_curation_coarse 3

devstage_curation 3

technology 2

experiment.platform 2

experiment.library_layout 2

tissue_curation_coarse 1

  • Reproductive System · 188 ✖

tissue_curation 1

experiment.library_strategy 1

experiment.library_source 1

experiment.library_selection 1

  • Oligo-dT · 188 ✖
Link rowid ▼ run.accession experiment.accession sample.accession study.accession bioproject study.title study.alias study.type study.abstract study.attributes study.PMIDs sample.description sample.title sample.alias sample.centername sample.attributes GEOsample.title GEOsample.dataprocessing GEOsample.source GEOsample.treatmentprotocol GEOsample.extractprotocol GEOsample.growthprotocol GEOsample.characteristics GEOsample.accession experiment.title experiment.alias experiment.library_name experiment.design_description experiment.library_construction_protocol experiment.attributes experiment.library_strategy experiment.library_source experiment.library_selection experiment.library_layout experiment.platform experiment.instrument_model experiment.spot_descriptor experiment.study_ref run.title run.attributes run.filename run.semantic_name run.total_bases run.total_spots run.alias run.read_lengths run.base_counts run.r1_length run.r2_length run.r3_length run.r4_length run.Acount run.Ccount run.Gcount run.Tcount run.Ncount run.experiment run.pool_member submission.accession submission.srasource submission.bioprojectsource seqdetective.n_mates seqdetective.mapping_rate.mate1 seqdetective.mapping_rate.mate2 seqdetective.nofeature_rate.mate1 seqdetective.nofeature_rate.mate2 seqdetective.sparsity.mate1 seqdetective.sparsity.mate2 seqdetective.pos_strand_rate.mate1 seqdetective.pos_strand_rate.mate2 seqdetective.readlen.mate1 seqdetective.readlen.mate2 seqdetective.judgement.mate1 seqdetective.judgement.mate2 seqdetective.judgement.reason platform_family instrument_generation read_bias selection_class prep_kit sc_or_bulk tech_class technology tech_variant submission.bioprojectsource.country earliest_date devstage_curation devstage_curation_coarse tissue_curation tissue_curation_coarse
9357 9357 ERR2983451 ERX2986067 ERS2955655 ERP112513 PRJEB30097 RNA Seq of Danionine species E-MTAB-7476 Transcriptome Analysis Tissues specific transcriptomes of Danio rerio Danio albolineatus and Danio aesculapii Brain Liver and Gonads. ENA FIRST PUBLIC:2018 12 05|ENA LAST UPDATE:2018 12 05   Protocols: Organs were sampled and snap frozen in liquid nitrogen RNA was extracted using Trizol and standard silica membrane protocol Library was constructed using magnetic beads dT to enrich mRNA. post fragmentation the cDNA was synthesized using the mRNA fragments as templates with random hexamer primers. R2wG SAMEA5147910 University of Hamburg, Institute of Zoology, Molecular Animal Physiology ENA FIRST PUBLIC:2018 12 05T17:02:27Z|ENA LAST UPDATE:2018 12 05T10:01:48Z|External Id:SAMEA5147910|INSDC center name:University of Hamburg Institute of Zoology Molecular Animal Physiology|INSDC first public:2018 12 05T17:02:27Z|INSDC last update:2018 12 05T10:01:48Z|INSDC status:public|Submitter Id:E MTAB 7476:R2wG|age:3|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|genotype:wild type genotype|individual:2|organism part:gonad|sample name:E MTAB 7476:R2wG|scientific name:Danio rerio|sex:female|strain:tu                 Illumina HiSeq 4000 paired end sequencing; RNA Seq of Danio9 species E MTAB 7476:R2wG p R2wG p RNA Seq of Danionine species Organs were sampled and snap frozen in liquid nitrogen RNA was extracted using Trizol and standard silica membrane protocol Library was constructed using magnetic beads dT to enrich mRNA. post fragmentation the cDNA was synthesized using the mRNA fragments as templates with random hexamer primers. Experimental Factor: organism:Danio rerio|Experimental Factor: organism part:gonad RNA-Seq TRANSCRIPTOMIC Oligo-dT PAIRED ILLUMINA Illumina HiSeq 4000 <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>200</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>101</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> ERP112513 Illumina HiSeq 4000 paired end sequencing; RNA Seq of Danionine species ENA FIRST PUBLIC:2018 12 05|ENA LAST UPDATE:2018 12 05 FCHNW2WBBXX_L6_HKZEBodsEAAFRAAPEI-209_1.fq.gz FCHNW2WBBXX_L6_HKZEBodsEAAFRAAPEI-209_2.fq.gz fastq fastq 4276100600.0 21380503.0 E MTAB 7476:FCHNW2WBBXX L6 HKZEBodsEAAFRAAPEI 209 0:100 1:100 A:1108098396;C:1033423434;G:1019885778;T:1113782383;N:910609 100 100     1108098396 1033423434 1019885778 1113782383 910609 ERX2986067 ERS2955655 ERA1674470 University of Hamburg, Institute of Zoology, Molecular Animal Physiology|European Nucleotide Archive University of Hamburg, Institute of Zoology, Molecular Animal Physiology|European Nucleotide Archive 2 0.9444 0.94495 0.02624 0.02606 0.74959 0.7512 0.48453 0.48539 100 100 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   Germany 2018-12-05 Adult Adult Gonad Reproductive System
9359 9359 ERR2983449 ERX2986065 ERS2955653 ERP112513 PRJEB30097 RNA Seq of Danionine species E-MTAB-7476 Transcriptome Analysis Tissues specific transcriptomes of Danio rerio Danio albolineatus and Danio aesculapii Brain Liver and Gonads. ENA FIRST PUBLIC:2018 12 05|ENA LAST UPDATE:2018 12 05   Protocols: Organs were sampled and snap frozen in liquid nitrogen RNA was extracted using Trizol and standard silica membrane protocol Library was constructed using magnetic beads dT to enrich mRNA. post fragmentation the cDNA was synthesized using the mRNA fragments as templates with random hexamer primers. R1wG SAMEA5147908 University of Hamburg, Institute of Zoology, Molecular Animal Physiology ENA FIRST PUBLIC:2018 12 05T17:02:27Z|ENA LAST UPDATE:2018 12 05T10:01:48Z|External Id:SAMEA5147908|INSDC center name:University of Hamburg Institute of Zoology Molecular Animal Physiology|INSDC first public:2018 12 05T17:02:27Z|INSDC last update:2018 12 05T10:01:48Z|INSDC status:public|Submitter Id:E MTAB 7476:R1wG|age:3|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|genotype:wild type genotype|individual:1|organism part:gonad|sample name:E MTAB 7476:R1wG|scientific name:Danio rerio|sex:female|strain:tu                 Illumina HiSeq 4000 paired end sequencing; RNA Seq of Danio9 species E MTAB 7476:R1wG p R1wG p RNA Seq of Danionine species Organs were sampled and snap frozen in liquid nitrogen RNA was extracted using Trizol and standard silica membrane protocol Library was constructed using magnetic beads dT to enrich mRNA. post fragmentation the cDNA was synthesized using the mRNA fragments as templates with random hexamer primers. Experimental Factor: organism:Danio rerio|Experimental Factor: organism part:gonad RNA-Seq TRANSCRIPTOMIC Oligo-dT PAIRED ILLUMINA Illumina HiSeq 4000 <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>200</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>101</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> ERP112513 Illumina HiSeq 4000 paired end sequencing; RNA Seq of Danionine species ENA FIRST PUBLIC:2018 12 05|ENA LAST UPDATE:2018 12 05 FCHNW2WBBXX_L6_HKZEBodsEAAERAAPEI-208_1.fq.gz FCHNW2WBBXX_L6_HKZEBodsEAAERAAPEI-208_2.fq.gz fastq fastq 3574419200.0 17872096.0 E MTAB 7476:FCHNW2WBBXX L6 HKZEBodsEAAERAAPEI 208 0:100 1:100 A:927454175;C:863033369;G:844910972;T:938253904;N:766780 100 100     927454175 863033369 844910972 938253904 766780 ERX2986065 ERS2955653 ERA1674470 University of Hamburg, Institute of Zoology, Molecular Animal Physiology|European Nucleotide Archive University of Hamburg, Institute of Zoology, Molecular Animal Physiology|European Nucleotide Archive 2 0.93851 0.93973 0.02799 0.02809 0.72701 0.72914 0.48827 0.48841 100 100 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   Germany 2018-12-05 Adult Adult Gonad Reproductive System
26542 26542 SRR28370185 SRX23975270 SRS20773688 SRP496008 PRJNA1020641 Danio rerio strain:AB strain Raw sequence reads PRJNA1020641 Whole Genome Sequencing post different low temperature treatments for 14 days transcriptome sequencing was conducted on ovaries to analyze the metabolic state under low temperatures.         L13 2 1   strain:not applicable|age:7|dev stage:month|collection date:2021 08 23|geo loc name:not applicable|sex:female|tissue:ovary|replicate:replicate=biological replicate 27|BioSampleModel:Model organism or animal                 RNA seq of ovary: adult female zebrafish E27 E27 Strand specific RNA seq libraries were prepared using the VAHTS Stranded mRNA seq Library Prep Kit for Illumina v2 Vazyme Biotech China and subjected to 150bp paired end sequencing on the Illumina Novaseq 6000 platform. Trimmomatic software was employed to remove low quality reads and sequencing adapters followed by the alignment of clean reads to the zebrafish reference genome GRCz11 v106 using STAR software. Read counts for each gene were calculated using featureCounts.     RNA-Seq TRANSCRIPTOMIC Oligo-dT PAIRED ILLUMINA Illumina NovaSeq 6000   SRP496008     L13-2_1.fq.gz L13-2_2.fq.gz fastq fastq 6912432900.0 23041443.0 L13 2 1.fq.gz 0:150 1:150 A:1784119910;C:1650041348;G:1690584500;T:1787634630;N:52512 150 150     1784119910 1650041348 1690584500 1787634630 52512 SRX23975270 SRS20773688 SRA1825755 shanghai ocean university|College of Fisheries and Life Science shanghai ocean university                       B B biological fallback assumption illumina novaseq_era unknown poly_a unknown bulk unknown unknown   China 2024-03-18 Adult Adult Gonad Reproductive System
26543 26543 SRR28370183 SRX23975269 SRS20773687 SRP496008 PRJNA1020641 Danio rerio strain:AB strain Raw sequence reads PRJNA1020641 Whole Genome Sequencing post different low temperature treatments for 14 days transcriptome sequencing was conducted on ovaries to analyze the metabolic state under low temperatures.         L13 3 1   strain:not applicable|age:7|dev stage:month|collection date:2021 08 23|geo loc name:not applicable|sex:female|tissue:ovary|replicate:replicate=biological replicate 29|BioSampleModel:Model organism or animal                 RNA seq of ovary: adult female zebrafish E29 E29 Strand specific RNA seq libraries were prepared using the VAHTS Stranded mRNA seq Library Prep Kit for Illumina v2 Vazyme Biotech China and subjected to 150bp paired end sequencing on the Illumina Novaseq 6000 platform. Trimmomatic software was employed to remove low quality reads and sequencing adapters followed by the alignment of clean reads to the zebrafish reference genome GRCz11 v106 using STAR software. Read counts for each gene were calculated using featureCounts.     RNA-Seq TRANSCRIPTOMIC Oligo-dT PAIRED ILLUMINA Illumina NovaSeq 6000   SRP496008     L13-3_1.fq.gz L13-3_2.fq.gz fastq fastq 6353525700.0 21178419.0 L13 3 1.fq.gz 0:150 1:150 A:1642863679;C:1514378445;G:1549765145;T:1646469683;N:48748 150 150     1642863679 1514378445 1549765145 1646469683 48748 SRX23975269 SRS20773687 SRA1825755 shanghai ocean university|College of Fisheries and Life Science shanghai ocean university                       B B biological fallback assumption illumina novaseq_era unknown poly_a unknown bulk unknown unknown   China 2024-03-18 Adult Adult Gonad Reproductive System
26544 26544 SRR28370186 SRX23975268 SRS20773685 SRP496008 PRJNA1020641 Danio rerio strain:AB strain Raw sequence reads PRJNA1020641 Whole Genome Sequencing post different low temperature treatments for 14 days transcriptome sequencing was conducted on ovaries to analyze the metabolic state under low temperatures.         L13 4 1   strain:not applicable|age:7|dev stage:month|collection date:2021 08 23|geo loc name:not applicable|sex:female|tissue:ovary|replicate:replicate=biological replicate 31|BioSampleModel:Model organism or animal                 RNA seq of ovary: adult female zebrafish E31 E31 Strand specific RNA seq libraries were prepared using the VAHTS Stranded mRNA seq Library Prep Kit for Illumina v2 Vazyme Biotech China and subjected to 150bp paired end sequencing on the Illumina Novaseq 6000 platform. Trimmomatic software was employed to remove low quality reads and sequencing adapters followed by the alignment of clean reads to the zebrafish reference genome GRCz11 v106 using STAR software. Read counts for each gene were calculated using featureCounts.     RNA-Seq TRANSCRIPTOMIC Oligo-dT PAIRED ILLUMINA Illumina NovaSeq 6000   SRP496008     L13-4_1.fq.gz L13-4_2.fq.gz fastq fastq 6026853600.0 20089512.0 L13 4 1.fq.gz 0:150 1:150 A:1557631540;C:1437822618;G:1472097382;T:1559255786;N:46274 150 150     1557631540 1437822618 1472097382 1559255786 46274 SRX23975268 SRS20773685 SRA1825755 shanghai ocean university|College of Fisheries and Life Science shanghai ocean university                       B B biological fallback assumption illumina novaseq_era unknown poly_a unknown bulk unknown unknown   China 2024-03-18 Adult Adult Gonad Reproductive System
26545 26545 SRR28370187 SRX23975267 SRS20773686 SRP496008 PRJNA1020641 Danio rerio strain:AB strain Raw sequence reads PRJNA1020641 Whole Genome Sequencing post different low temperature treatments for 14 days transcriptome sequencing was conducted on ovaries to analyze the metabolic state under low temperatures.         H27 3 1   strain:not applicable|age:7|dev stage:month|collection date:2021 08 23|geo loc name:not applicable|sex:female|tissue:ovary|replicate:replicate=biological replicate 5|BioSampleModel:Model organism or animal                 RNA seq of ovary: adult female zebrafish E5 E5 Strand specific RNA seq libraries were prepared using the VAHTS Stranded mRNA seq Library Prep Kit for Illumina v2 Vazyme Biotech China and subjected to 150bp paired end sequencing on the Illumina Novaseq 6000 platform. Trimmomatic software was employed to remove low quality reads and sequencing adapters followed by the alignment of clean reads to the zebrafish reference genome GRCz11 v106 using STAR software. Read counts for each gene were calculated using featureCounts.     RNA-Seq TRANSCRIPTOMIC Oligo-dT PAIRED ILLUMINA Illumina NovaSeq 6000   SRP496008     H27-3_1.fq.gz H27-3_2.fq.gz fastq fastq 6437512500.0 21458375.0 H27 3 1.fq.gz 0:150 1:150 A:1664818485;C:1534913781;G:1570911516;T:1666819538;N:49180 150 150     1664818485 1534913781 1570911516 1666819538 49180 SRX23975267 SRS20773686 SRA1825755 shanghai ocean university|College of Fisheries and Life Science shanghai ocean university                       B B biological fallback assumption illumina novaseq_era unknown poly_a unknown bulk unknown unknown   China 2024-03-18 Adult Adult Gonad Reproductive System
26546 26546 SRR28370188 SRX23975266 SRS20773684 SRP496008 PRJNA1020641 Danio rerio strain:AB strain Raw sequence reads PRJNA1020641 Whole Genome Sequencing post different low temperature treatments for 14 days transcriptome sequencing was conducted on ovaries to analyze the metabolic state under low temperatures.         H27 4 1   strain:not applicable|age:7|dev stage:month|collection date:2021 08 23|geo loc name:not applicable|sex:female|tissue:ovary|replicate:replicate=biological replicate 7|BioSampleModel:Model organism or animal                 RNA seq of ovary: adult female zebrafish E7 E7 Strand specific RNA seq libraries were prepared using the VAHTS Stranded mRNA seq Library Prep Kit for Illumina v2 Vazyme Biotech China and subjected to 150bp paired end sequencing on the Illumina Novaseq 6000 platform. Trimmomatic software was employed to remove low quality reads and sequencing adapters followed by the alignment of clean reads to the zebrafish reference genome GRCz11 v106 using STAR software. Read counts for each gene were calculated using featureCounts.     RNA-Seq TRANSCRIPTOMIC Oligo-dT PAIRED ILLUMINA Illumina NovaSeq 6000   SRP496008     H27-4_1.fq.gz H27-4_2.fq.gz fastq fastq 6583604100.0 21945347.0 H27 4 1.fq.gz 0:150 1:150 A:1701207657;C:1570727535;G:1607990863;T:1703626924;N:51121 150 150     1701207657 1570727535 1607990863 1703626924 51121 SRX23975266 SRS20773684 SRA1825755 shanghai ocean university|College of Fisheries and Life Science shanghai ocean university                       B B biological fallback assumption illumina novaseq_era unknown poly_a unknown bulk unknown unknown   China 2024-03-18 Adult Adult Gonad Reproductive System
26547 26547 SRR28370189 SRX23975265 SRS20773683 SRP496008 PRJNA1020641 Danio rerio strain:AB strain Raw sequence reads PRJNA1020641 Whole Genome Sequencing post different low temperature treatments for 14 days transcriptome sequencing was conducted on ovaries to analyze the metabolic state under low temperatures.         M20 1 1   strain:not applicable|age:7|dev stage:month|collection date:2021 08 23|geo loc name:not applicable|sex:female|tissue:ovary|replicate:replicate=biological replicate 9|BioSampleModel:Model organism or animal                 RNA seq of ovary: adult female zebrafish E9 E9 Strand specific RNA seq libraries were prepared using the VAHTS Stranded mRNA seq Library Prep Kit for Illumina v2 Vazyme Biotech China and subjected to 150bp paired end sequencing on the Illumina Novaseq 6000 platform. Trimmomatic software was employed to remove low quality reads and sequencing adapters followed by the alignment of clean reads to the zebrafish reference genome GRCz11 v106 using STAR software. Read counts for each gene were calculated using featureCounts.     RNA-Seq TRANSCRIPTOMIC Oligo-dT PAIRED ILLUMINA Illumina NovaSeq 6000   SRP496008     M20-1_1.fq.gz M20-1_2.fq.gz fastq fastq 7048815900.0 23496053.0 M20 1 1.fq.gz 0:150 1:150 A:1818915133;C:1681912688;G:1724222526;T:1823711995;N:53558 150 150     1818915133 1681912688 1724222526 1823711995 53558 SRX23975265 SRS20773683 SRA1825755 shanghai ocean university|College of Fisheries and Life Science shanghai ocean university                       B B biological fallback assumption illumina novaseq_era unknown poly_a unknown bulk unknown unknown   China 2024-03-18 Adult Adult Gonad Reproductive System
26548 26548 SRR28370190 SRX23975264 SRS20773682 SRP496008 PRJNA1020641 Danio rerio strain:AB strain Raw sequence reads PRJNA1020641 Whole Genome Sequencing post different low temperature treatments for 14 days transcriptome sequencing was conducted on ovaries to analyze the metabolic state under low temperatures.         M22 2 1   strain:not applicable|age:7|dev stage:month|collection date:2021 08 23|geo loc name:not applicable|sex:female|tissue:ovary|replicate:replicate=biological replicate 19|BioSampleModel:Model organism or animal                 RNA seq of ovary: adult female zebrafish E19 E19 Strand specific RNA seq libraries were prepared using the VAHTS Stranded mRNA seq Library Prep Kit for Illumina v2 Vazyme Biotech China and subjected to 150bp paired end sequencing on the Illumina Novaseq 6000 platform. Trimmomatic software was employed to remove low quality reads and sequencing adapters followed by the alignment of clean reads to the zebrafish reference genome GRCz11 v106 using STAR software. Read counts for each gene were calculated using featureCounts.     RNA-Seq TRANSCRIPTOMIC Oligo-dT PAIRED ILLUMINA Illumina NovaSeq 6000   SRP496008     M22-2_1.fq.gz M22-2_2.fq.gz fastq fastq 6844400100.0 22814667.0 M22 2 1.fq.gz 0:150 1:150 A:1770073015;C:1631597148;G:1666105661;T:1776572883;N:51393 150 150     1770073015 1631597148 1666105661 1776572883 51393 SRX23975264 SRS20773682 SRA1825755 shanghai ocean university|College of Fisheries and Life Science shanghai ocean university                       B B biological fallback assumption illumina novaseq_era unknown poly_a unknown bulk unknown unknown   China 2024-03-18 Adult Adult Gonad Reproductive System
26549 26549 SRR28370191 SRX23975263 SRS20773681 SRP496008 PRJNA1020641 Danio rerio strain:AB strain Raw sequence reads PRJNA1020641 Whole Genome Sequencing post different low temperature treatments for 14 days transcriptome sequencing was conducted on ovaries to analyze the metabolic state under low temperatures.         H27 2 1   strain:not applicable|age:7|dev stage:month|collection date:2021 08 23|geo loc name:not applicable|sex:female|tissue:ovary|replicate:replicate=biological replicate 3|BioSampleModel:Model organism or animal                 RNA seq of ovary: adult female zebrafish E3 E3 Strand specific RNA seq libraries were prepared using the VAHTS Stranded mRNA seq Library Prep Kit for Illumina v2 Vazyme Biotech China and subjected to 150bp paired end sequencing on the Illumina Novaseq 6000 platform. Trimmomatic software was employed to remove low quality reads and sequencing adapters followed by the alignment of clean reads to the zebrafish reference genome GRCz11 v106 using STAR software. Read counts for each gene were calculated using featureCounts.     RNA-Seq TRANSCRIPTOMIC Oligo-dT PAIRED ILLUMINA Illumina NovaSeq 6000   SRP496008     H27-2_1.fq.gz H27-2_2.fq.gz fastq fastq 6803741100.0 22679137.0 H27 2 1.fq.gz 0:150 1:150 A:1759471720;C:1621328873;G:1660379342;T:1762509905;N:51260 150 150     1759471720 1621328873 1660379342 1762509905 51260 SRX23975263 SRS20773681 SRA1825755 shanghai ocean university|College of Fisheries and Life Science shanghai ocean university                       B B biological fallback assumption illumina novaseq_era unknown poly_a unknown bulk unknown unknown   China 2024-03-18 Adult Adult Gonad Reproductive System
26550 26550 SRR28370184 SRX23975262 SRS20773680 SRP496008 PRJNA1020641 Danio rerio strain:AB strain Raw sequence reads PRJNA1020641 Whole Genome Sequencing post different low temperature treatments for 14 days transcriptome sequencing was conducted on ovaries to analyze the metabolic state under low temperatures.         M22 3 1   strain:not applicable|age:7|dev stage:month|collection date:2021 08 23|geo loc name:not applicable|sex:female|tissue:ovary|replicate:replicate=biological replicate 21|BioSampleModel:Model organism or animal                 RNA seq of ovary: adult female zebrafish E21 E21 Strand specific RNA seq libraries were prepared using the VAHTS Stranded mRNA seq Library Prep Kit for Illumina v2 Vazyme Biotech China and subjected to 150bp paired end sequencing on the Illumina Novaseq 6000 platform. Trimmomatic software was employed to remove low quality reads and sequencing adapters followed by the alignment of clean reads to the zebrafish reference genome GRCz11 v106 using STAR software. Read counts for each gene were calculated using featureCounts.     RNA-Seq TRANSCRIPTOMIC Oligo-dT PAIRED ILLUMINA Illumina NovaSeq 6000   SRP496008     M22-3_1.fq.gz M22-3_2.fq.gz fastq fastq 6312346800.0 21041156.0 M22 3 1.fq.gz 0:150 1:150 A:1638152183;C:1500304435;G:1533591340;T:1640250617;N:48225 150 150     1638152183 1500304435 1533591340 1640250617 48225 SRX23975262 SRS20773680 SRA1825755 shanghai ocean university|College of Fisheries and Life Science shanghai ocean university                       B B biological fallback assumption illumina novaseq_era unknown poly_a unknown bulk unknown unknown   China 2024-03-18 Adult Adult Gonad Reproductive System
26551 26551 SRR28370192 SRX23975261 SRS20773679 SRP496008 PRJNA1020641 Danio rerio strain:AB strain Raw sequence reads PRJNA1020641 Whole Genome Sequencing post different low temperature treatments for 14 days transcriptome sequencing was conducted on ovaries to analyze the metabolic state under low temperatures.         M22 4 1   strain:not applicable|age:7|dev stage:month|collection date:2021 08 23|geo loc name:not applicable|sex:female|tissue:ovary|replicate:replicate=biological replicate 23|BioSampleModel:Model organism or animal                 RNA seq of ovary: adult female zebrafish E23 E23 Strand specific RNA seq libraries were prepared using the VAHTS Stranded mRNA seq Library Prep Kit for Illumina v2 Vazyme Biotech China and subjected to 150bp paired end sequencing on the Illumina Novaseq 6000 platform. Trimmomatic software was employed to remove low quality reads and sequencing adapters followed by the alignment of clean reads to the zebrafish reference genome GRCz11 v106 using STAR software. Read counts for each gene were calculated using featureCounts.     RNA-Seq TRANSCRIPTOMIC Oligo-dT PAIRED ILLUMINA Illumina NovaSeq 6000   SRP496008     M22-4_1.fq.gz M22-4_2.fq.gz fastq fastq 7163095500.0 23876985.0 M22 4 1.fq.gz 0:150 1:150 A:1851012240;C:1707016293;G:1748790263;T:1856222899;N:53805 150 150     1851012240 1707016293 1748790263 1856222899 53805 SRX23975261 SRS20773679 SRA1825755 shanghai ocean university|College of Fisheries and Life Science shanghai ocean university                       B B biological fallback assumption illumina novaseq_era unknown poly_a unknown bulk unknown unknown   China 2024-03-18 Adult Adult Gonad Reproductive System
26552 26552 SRR28370193 SRX23975260 SRS20773678 SRP496008 PRJNA1020641 Danio rerio strain:AB strain Raw sequence reads PRJNA1020641 Whole Genome Sequencing post different low temperature treatments for 14 days transcriptome sequencing was conducted on ovaries to analyze the metabolic state under low temperatures.         L13 1 1   strain:not applicable|age:7|dev stage:month|collection date:2021 08 23|geo loc name:not applicable|sex:female|tissue:ovary|replicate:replicate=biological replicate 25|BioSampleModel:Model organism or animal                 RNA seq of ovary: adult female zebrafish E25 E25 Strand specific RNA seq libraries were prepared using the VAHTS Stranded mRNA seq Library Prep Kit for Illumina v2 Vazyme Biotech China and subjected to 150bp paired end sequencing on the Illumina Novaseq 6000 platform. Trimmomatic software was employed to remove low quality reads and sequencing adapters followed by the alignment of clean reads to the zebrafish reference genome GRCz11 v106 using STAR software. Read counts for each gene were calculated using featureCounts.     RNA-Seq TRANSCRIPTOMIC Oligo-dT PAIRED ILLUMINA Illumina NovaSeq 6000   SRP496008     L13-1_1.fq.gz L13-1_2.fq.gz fastq fastq 6191424300.0 20638081.0 L13 1 1.fq.gz 0:150 1:150 A:1605753886;C:1471890545;G:1506901769;T:1606830995;N:47105 150 150     1605753886 1471890545 1506901769 1606830995 47105 SRX23975260 SRS20773678 SRA1825755 shanghai ocean university|College of Fisheries and Life Science shanghai ocean university                       B B biological fallback assumption illumina novaseq_era unknown poly_a unknown bulk unknown unknown   China 2024-03-18 Adult Adult Gonad Reproductive System
26553 26553 SRR28370194 SRX23975259 SRS20773677 SRP496008 PRJNA1020641 Danio rerio strain:AB strain Raw sequence reads PRJNA1020641 Whole Genome Sequencing post different low temperature treatments for 14 days transcriptome sequencing was conducted on ovaries to analyze the metabolic state under low temperatures.         M22 1 1   strain:not applicable|age:7|dev stage:month|collection date:2021 08 23|geo loc name:not applicable|sex:female|tissue:ovary|replicate:replicate=biological replicate 17|BioSampleModel:Model organism or animal                 RNA seq of ovary: adult female zebrafish E17 E17 Strand specific RNA seq libraries were prepared using the VAHTS Stranded mRNA seq Library Prep Kit for Illumina v2 Vazyme Biotech China and subjected to 150bp paired end sequencing on the Illumina Novaseq 6000 platform. Trimmomatic software was employed to remove low quality reads and sequencing adapters followed by the alignment of clean reads to the zebrafish reference genome GRCz11 v106 using STAR software. Read counts for each gene were calculated using featureCounts.     RNA-Seq TRANSCRIPTOMIC Oligo-dT PAIRED ILLUMINA Illumina NovaSeq 6000   SRP496008     M22-1_1.fq.gz M22-1_2.fq.gz fastq fastq 6102383400.0 20341278.0 M22 1 1.fq.gz 0:150 1:150 A:1577012976;C:1455984420;G:1487747979;T:1581590987;N:47038 150 150     1577012976 1455984420 1487747979 1581590987 47038 SRX23975259 SRS20773677 SRA1825755 shanghai ocean university|College of Fisheries and Life Science shanghai ocean university                       B B biological fallback assumption illumina novaseq_era unknown poly_a unknown bulk unknown unknown   China 2024-03-18 Adult Adult Gonad Reproductive System
26554 26554 SRR28370195 SRX23975258 SRS20773676 SRP496008 PRJNA1020641 Danio rerio strain:AB strain Raw sequence reads PRJNA1020641 Whole Genome Sequencing post different low temperature treatments for 14 days transcriptome sequencing was conducted on ovaries to analyze the metabolic state under low temperatures.         M20 4 1   strain:not applicable|age:7|dev stage:month|collection date:2021 08 23|geo loc name:not applicable|sex:female|tissue:ovary|replicate:replicate=biological replicate 15|BioSampleModel:Model organism or animal                 RNA seq of ovary: adult female zebrafish E15 E15 Strand specific RNA seq libraries were prepared using the VAHTS Stranded mRNA seq Library Prep Kit for Illumina v2 Vazyme Biotech China and subjected to 150bp paired end sequencing on the Illumina Novaseq 6000 platform. Trimmomatic software was employed to remove low quality reads and sequencing adapters followed by the alignment of clean reads to the zebrafish reference genome GRCz11 v106 using STAR software. Read counts for each gene were calculated using featureCounts.     RNA-Seq TRANSCRIPTOMIC Oligo-dT PAIRED ILLUMINA Illumina NovaSeq 6000   SRP496008     M20-4_1.fq.gz M20-4_2.fq.gz fastq fastq 6636566100.0 22121887.0 M20 4 1.fq.gz 0:150 1:150 A:1715667953;C:1580553403;G:1620418612;T:1719875870;N:50262 150 150     1715667953 1580553403 1620418612 1719875870 50262 SRX23975258 SRS20773676 SRA1825755 shanghai ocean university|College of Fisheries and Life Science shanghai ocean university                       B B biological fallback assumption illumina novaseq_era unknown poly_a unknown bulk unknown unknown   China 2024-03-18 Adult Adult Gonad Reproductive System
26555 26555 SRR28370196 SRX23975257 SRS20773675 SRP496008 PRJNA1020641 Danio rerio strain:AB strain Raw sequence reads PRJNA1020641 Whole Genome Sequencing post different low temperature treatments for 14 days transcriptome sequencing was conducted on ovaries to analyze the metabolic state under low temperatures.         M20 3 1   strain:not applicable|age:7|dev stage:month|collection date:2021 08 23|geo loc name:not applicable|sex:female|tissue:ovary|replicate:replicate=biological replicate 13|BioSampleModel:Model organism or animal                 RNA seq of ovary: adult female zebrafish E13 E13 Strand specific RNA seq libraries were prepared using the VAHTS Stranded mRNA seq Library Prep Kit for Illumina v2 Vazyme Biotech China and subjected to 150bp paired end sequencing on the Illumina Novaseq 6000 platform. Trimmomatic software was employed to remove low quality reads and sequencing adapters followed by the alignment of clean reads to the zebrafish reference genome GRCz11 v106 using STAR software. Read counts for each gene were calculated using featureCounts.     RNA-Seq TRANSCRIPTOMIC Oligo-dT PAIRED ILLUMINA Illumina NovaSeq 6000   SRP496008     M20-3_1.fq.gz M20-3_2.fq.gz fastq fastq 6761256900.0 22537523.0 M20 3 1.fq.gz 0:150 1:150 A:1758917520;C:1603264927;G:1642146940;T:1756875822;N:51691 150 150     1758917520 1603264927 1642146940 1756875822 51691 SRX23975257 SRS20773675 SRA1825755 shanghai ocean university|College of Fisheries and Life Science shanghai ocean university                       B B biological fallback assumption illumina novaseq_era unknown poly_a unknown bulk unknown unknown   China 2024-03-18 Adult Adult Gonad Reproductive System
26556 26556 SRR28370197 SRX23975256 SRS20773674 SRP496008 PRJNA1020641 Danio rerio strain:AB strain Raw sequence reads PRJNA1020641 Whole Genome Sequencing post different low temperature treatments for 14 days transcriptome sequencing was conducted on ovaries to analyze the metabolic state under low temperatures.         M20 2 1   strain:not applicable|age:7|dev stage:month|collection date:2021 08 23|geo loc name:not applicable|sex:female|tissue:ovary|replicate:replicate=biological replicate 11|BioSampleModel:Model organism or animal                 RNA seq of ovary: adult female zebrafish E11 E11 Strand specific RNA seq libraries were prepared using the VAHTS Stranded mRNA seq Library Prep Kit for Illumina v2 Vazyme Biotech China and subjected to 150bp paired end sequencing on the Illumina Novaseq 6000 platform. Trimmomatic software was employed to remove low quality reads and sequencing adapters followed by the alignment of clean reads to the zebrafish reference genome GRCz11 v106 using STAR software. Read counts for each gene were calculated using featureCounts.     RNA-Seq TRANSCRIPTOMIC Oligo-dT PAIRED ILLUMINA Illumina NovaSeq 6000   SRP496008     M20-2_1.fq.gz M20-2_2.fq.gz fastq fastq 6746623800.0 22488746.0 M20 2 1.fq.gz 0:150 1:150 A:1744481110;C:1606775717;G:1646048497;T:1749267538;N:50938 150 150     1744481110 1606775717 1646048497 1749267538 50938 SRX23975256 SRS20773674 SRA1825755 shanghai ocean university|College of Fisheries and Life Science shanghai ocean university                       B B biological fallback assumption illumina novaseq_era unknown poly_a unknown bulk unknown unknown   China 2024-03-18 Adult Adult Gonad Reproductive System
26557 26557 SRR28370198 SRX23975255 SRS20773673 SRP496008 PRJNA1020641 Danio rerio strain:AB strain Raw sequence reads PRJNA1020641 Whole Genome Sequencing post different low temperature treatments for 14 days transcriptome sequencing was conducted on ovaries to analyze the metabolic state under low temperatures.         H27 1 1   strain:not applicable|age:7|dev stage:month|collection date:2021 08 23|geo loc name:not applicable|sex:female|tissue:ovary|replicate:replicate=biological replicate 1|BioSampleModel:Model organism or animal                 RNA seq of ovary: adult female zebrafish E1 E1 Strand specific RNA seq libraries were prepared using the VAHTS Stranded mRNA seq Library Prep Kit for Illumina v2 Vazyme Biotech China and subjected to 150bp paired end sequencing on the Illumina Novaseq 6000 platform. Trimmomatic software was employed to remove low quality reads and sequencing adapters followed by the alignment of clean reads to the zebrafish reference genome GRCz11 v106 using STAR software. Read counts for each gene were calculated using featureCounts.     RNA-Seq TRANSCRIPTOMIC Oligo-dT PAIRED ILLUMINA Illumina NovaSeq 6000   SRP496008     H27-1_1.fq.gz H27-1_2.fq.gz fastq fastq 6713598900.0 22378663.0 H27 1 1.fq.gz 0:150 1:150 A:1734915716;C:1602520747;G:1639382442;T:1736729508;N:50487 150 150     1734915716 1602520747 1639382442 1736729508 50487 SRX23975255 SRS20773673 SRA1825755 shanghai ocean university|College of Fisheries and Life Science shanghai ocean university                       B B biological fallback assumption illumina novaseq_era unknown poly_a unknown bulk unknown unknown   China 2024-03-18 Adult Adult Gonad Reproductive System
28800 28800 SRR26744390 SRX22443207 SRS19460925 SRP470937 PRJNA1036532 Danio rerio Raw sequence reads PRJNA1036532 Whole Genome Sequencing normal RNAseq of zebrafish       TL1 AWG21KI956   strain:AB wild type|age:4 mpf|collection date:2021 10 20|geo loc name:China|sex:male|tissue:testis|BioSampleModel:Model organism or animal                 RNAseq of zebrafish LM LM normal RNAseq of zebrafish     RNA-Seq TRANSCRIPTOMIC Oligo-dT PAIRED ILLUMINA Illumina HiSeq 4000   SRP470937     TL1_2.clean.fq.gz TL1_1.clean.fq.gz fastq fastq 5699083800.0 18996946.0 TL1 1.clean.fq.gz 0:150 1:150 A:1551237534;C:1296027828;G:1322262120;T:1529547653;N:8665 150 150     1551237534 1296027828 1322262120 1529547653 8665 SRX22443207 SRS19460925 SRA1747895 Fujian Agriculture and Forestry University|College of Marine Sciences Fujian Agriculture and Forestry University 2 0.92378 0.91959 0.10498 0.1026 0.6299 0.64319 0.49163 0.48651 150 150 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   China 2023-11-09 Adult Adult Gonad Reproductive System
28801 28801 SRR26744391 SRX22443206 SRS19460924 SRP470937 PRJNA1036532 Danio rerio Raw sequence reads PRJNA1036532 Whole Genome Sequencing normal RNAseq of zebrafish       OL3 AWG21KN131   strain:AB wild type|age:4 mpf|collection date:2021 10 19|geo loc name:China|sex:female|tissue:ovary|BioSampleModel:Model organism or animal                 RNAseq of zebrafish LF3 LF3 normal RNAseq of zebrafish     RNA-Seq TRANSCRIPTOMIC Oligo-dT PAIRED ILLUMINA Illumina HiSeq 4000   SRP470937     OL3_2.clean.fq.gz OL3_1.clean.fq.gz fastq fastq 6314250900.0 21047503.0 OL3 1.clean.fq.gz 0:150 1:150 A:1645668992;C:1501216557;G:1526087917;T:1641268461;N:8973 150 150     1645668992 1501216557 1526087917 1641268461 8973 SRX22443206 SRS19460924 SRA1747895 Fujian Agriculture and Forestry University|College of Marine Sciences Fujian Agriculture and Forestry University 2 0.92555 0.92469 0.01845 0.01818 0.75146 0.75298 0.4697 0.47119 150 150 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   China 2023-11-09 Adult Adult Gonad Reproductive System
28802 28802 SRR26744392 SRX22443205 SRS19460920 SRP470937 PRJNA1036532 Danio rerio Raw sequence reads PRJNA1036532 Whole Genome Sequencing normal RNAseq of zebrafish       OL2 AWG21KN130   strain:AB wild type|age:4 mpf|collection date:2021 10 18|geo loc name:China|sex:female|tissue:ovary|BioSampleModel:Model organism or animal                 RNAseq of zebrafish LF2 LF2 normal RNAseq of zebrafish     RNA-Seq TRANSCRIPTOMIC Oligo-dT PAIRED ILLUMINA Illumina HiSeq 4000   SRP470937     OL2_1.clean.fq.gz OL2_2.clean.fq.gz fastq fastq 5723391300.0 19077971.0 OL2 1.clean.fq.gz 0:150 1:150 A:1493997463;C:1358589750;G:1376156813;T:1494638946;N:8328 150 150     1493997463 1358589750 1376156813 1494638946 8328 SRX22443205 SRS19460920 SRA1747895 Fujian Agriculture and Forestry University|College of Marine Sciences Fujian Agriculture and Forestry University 2 0.92147 0.92251 0.0187 0.01891 0.75077 0.75156 0.46983 0.46864 150 150 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   China 2023-11-09 Adult Adult Gonad Reproductive System
28803 28803 SRR26744393 SRX22443204 SRS19460923 SRP470937 PRJNA1036532 Danio rerio Raw sequence reads PRJNA1036532 Whole Genome Sequencing normal RNAseq of zebrafish       OL1 AWG21KI955   strain:AB wild type|age:4 mpf|collection date:2021 10 17|geo loc name:China|sex:female|tissue:ovary|BioSampleModel:Model organism or animal                 RNAseq of zebrafish LF LF normal RNAseq of zebrafish     RNA-Seq TRANSCRIPTOMIC Oligo-dT PAIRED ILLUMINA Illumina HiSeq 4000   SRP470937     OL1_1.clean.fq.gz OL1_2.clean.fq.gz fastq fastq 5769588600.0 19231962.0 OL1 1.clean.fq.gz 0:150 1:150 A:1506564191;C:1368903119;G:1393174106;T:1500937757;N:9427 150 150     1506564191 1368903119 1393174106 1500937757 9427 SRX22443204 SRS19460923 SRA1747895 Fujian Agriculture and Forestry University|College of Marine Sciences Fujian Agriculture and Forestry University 2 0.92622 0.92542 0.01753 0.01714 0.75694 0.7596 0.47038 0.46862 150 150 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   China 2023-11-09 Adult Adult Gonad Reproductive System
28804 28804 SRR26744394 SRX22443203 SRS19460921 SRP470937 PRJNA1036532 Danio rerio Raw sequence reads PRJNA1036532 Whole Genome Sequencing normal RNAseq of zebrafish       TC3 AWG21KN129   strain:AB wild type|age:4 mpf|collection date:2021 10 16|geo loc name:China|sex:male|tissue:testis|BioSampleModel:Model organism or animal                 RNAseq of zebrafish CM3 CM3 normal RNAseq of zebrafish     RNA-Seq TRANSCRIPTOMIC Oligo-dT PAIRED ILLUMINA Illumina HiSeq 4000   SRP470937     TC3_2.clean.fq.gz TC3_1.clean.fq.gz fastq fastq 5849082900.0 19496943.0 TC3 1.clean.fq.gz 0:150 1:150 A:1588589470;C:1336193891;G:1355933296;T:1568357820;N:8423 150 150     1588589470 1336193891 1355933296 1568357820 8423 SRX22443203 SRS19460921 SRA1747895 Fujian Agriculture and Forestry University|College of Marine Sciences Fujian Agriculture and Forestry University 2 0.9259 0.92501 0.09579 0.09483 0.62501 0.6266 0.49341 0.49677 150 150 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   China 2023-11-09 Adult Adult Gonad Reproductive System
28805 28805 SRR26744395 SRX22443202 SRS19460922 SRP470937 PRJNA1036532 Danio rerio Raw sequence reads PRJNA1036532 Whole Genome Sequencing normal RNAseq of zebrafish       TC2 AWG21KN128   strain:AB wild type|age:4 mpf|collection date:2021 10 15|geo loc name:China|sex:male|tissue:testis|BioSampleModel:Model organism or animal                 RNAseq of zebrafish CM2 CM2 normal RNAseq of zebrafish     RNA-Seq TRANSCRIPTOMIC Oligo-dT PAIRED ILLUMINA Illumina HiSeq 4000   SRP470937     TC2_2.clean.fq.gz TC2_1.clean.fq.gz fastq fastq 6060182100.0 20200607.0 TC2 1.clean.fq.gz 0:150 1:150 A:1640267244;C:1389270827;G:1407872392;T:1622763112;N:8525 150 150     1640267244 1389270827 1407872392 1622763112 8525 SRX22443202 SRS19460922 SRA1747895 Fujian Agriculture and Forestry University|College of Marine Sciences Fujian Agriculture and Forestry University 2 0.92414 0.9235 0.0895 0.08929 0.62528 0.62682 0.48981 0.48954 150 150 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   China 2023-11-09 Adult Adult Gonad Reproductive System
28806 28806 SRR26744396 SRX22443201 SRS19460918 SRP470937 PRJNA1036532 Danio rerio Raw sequence reads PRJNA1036532 Whole Genome Sequencing normal RNAseq of zebrafish       TC1 AWG21KI954   strain:AB wild type|age:4 mpf|collection date:2021 10 14|geo loc name:China|sex:male|tissue:testis|BioSampleModel:Model organism or animal                 RNAseq of zebrafish CM CM normal RNAseq of zebrafish     RNA-Seq TRANSCRIPTOMIC Oligo-dT PAIRED ILLUMINA Illumina HiSeq 4000   SRP470937     TC1_1.clean.fq.gz TC1_2.clean.fq.gz fastq fastq 5990926200.0 19969754.0 TC1 1.clean.fq.gz 0:150 1:150 A:1624594580;C:1370442060;G:1389854623;T:1606025586;N:9351 150 150     1624594580 1370442060 1389854623 1606025586 9351 SRX22443201 SRS19460918 SRA1747895 Fujian Agriculture and Forestry University|College of Marine Sciences Fujian Agriculture and Forestry University 2 0.92373 0.92279 0.09865 0.09887 0.62568 0.62889 0.49516 0.49165 150 150 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   China 2023-11-09 Adult Adult Gonad Reproductive System
28807 28807 SRR26744397 SRX22443200 SRS19460914 SRP470937 PRJNA1036532 Danio rerio Raw sequence reads PRJNA1036532 Whole Genome Sequencing normal RNAseq of zebrafish       OC3 AWG21KN127   strain:AB wild type|age:4 mpf|collection date:2021 10 13|geo loc name:China|sex:female|tissue:ovary|BioSampleModel:Model organism or animal                 RNAseq of zebrafish CF3 CF3 normal RNAseq of zebrafish     RNA-Seq TRANSCRIPTOMIC Oligo-dT PAIRED ILLUMINA Illumina HiSeq 4000   SRP470937     OC3_1.clean.fq.gz OC3_2.clean.fq.gz fastq fastq 6016914600.0 20056382.0 OC3 1.clean.fq.gz 0:150 1:150 A:1568359804;C:1431107464;G:1450792678;T:1566645836;N:8818 150 150     1568359804 1431107464 1450792678 1566645836 8818 SRX22443200 SRS19460914 SRA1747895 Fujian Agriculture and Forestry University|College of Marine Sciences Fujian Agriculture and Forestry University 2 0.92534 0.92528 0.01718 0.017 0.75201 0.753 0.47491 0.48041 150 150 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   China 2023-11-09 Adult Adult Gonad Reproductive System
28808 28808 SRR26744398 SRX22443199 SRS19460915 SRP470937 PRJNA1036532 Danio rerio Raw sequence reads PRJNA1036532 Whole Genome Sequencing normal RNAseq of zebrafish       TL3 AWG21KN137   strain:AB wild type|age:4 mpf|collection date:2021 10 28|geo loc name:China|sex:male|tissue:testis|BioSampleModel:Model organism or animal                 RNAseq of zebrafish HM3 HM3 normal RNAseq of zebrafish     RNA-Seq TRANSCRIPTOMIC Oligo-dT PAIRED ILLUMINA Illumina HiSeq 4000   SRP470937     TH3_1.clean.fq.gz TH3_2.clean.fq.gz fastq fastq 5656023900.0 18853413.0 TH3 1.clean.fq.gz 0:150 1:150 A:1543170407;C:1285816998;G:1307460034;T:1519567327;N:9134 150 150     1543170407 1285816998 1307460034 1519567327 9134 SRX22443199 SRS19460915 SRA1747895 Fujian Agriculture and Forestry University|College of Marine Sciences Fujian Agriculture and Forestry University 2 0.92316 0.92166 0.10167 0.10105 0.61546 0.61712 0.482 0.4844 150 150 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   China 2023-11-09 Adult Adult Gonad Reproductive System
28809 28809 SRR26744399 SRX22443198 SRS19460919 SRP470937 PRJNA1036532 Danio rerio Raw sequence reads PRJNA1036532 Whole Genome Sequencing normal RNAseq of zebrafish       TL2 AWG21KN136   strain:AB wild type|age:4 mpf|collection date:2021 10 27|geo loc name:China|sex:male|tissue:testis|BioSampleModel:Model organism or animal                 RNAseq of zebrafish HM2 HM2 normal RNAseq of zebrafish     RNA-Seq TRANSCRIPTOMIC Oligo-dT PAIRED ILLUMINA Illumina HiSeq 4000   SRP470937     TH2_2.clean.fq.gz TH2_1.clean.fq.gz fastq fastq 6625748400.0 22085828.0 TH2 1.clean.fq.gz 0:150 1:150 A:1808454203;C:1505432915;G:1526482662;T:1785369099;N:9521 150 150     1808454203 1505432915 1526482662 1785369099 9521 SRX22443198 SRS19460919 SRA1747895 Fujian Agriculture and Forestry University|College of Marine Sciences Fujian Agriculture and Forestry University 2 0.92273 0.92286 0.09974 0.09973 0.62043 0.62203 0.48636 0.48603 150 150 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   China 2023-11-09 Adult Adult Gonad Reproductive System
28810 28810 SRR26744400 SRX22443197 SRS19460917 SRP470937 PRJNA1036532 Danio rerio Raw sequence reads PRJNA1036532 Whole Genome Sequencing normal RNAseq of zebrafish       TL1 AWG21KI958   strain:AB wild type|age:4 mpf|collection date:2021 10 26|geo loc name:China|sex:male|tissue:testis|BioSampleModel:Model organism or animal                 RNAseq of zebrafish HM HM normal RNAseq of zebrafish     RNA-Seq TRANSCRIPTOMIC Oligo-dT PAIRED ILLUMINA Illumina HiSeq 4000   SRP470937     TH1_1.clean.fq.gz TH1_2.clean.fq.gz fastq fastq 6588309600.0 21961032.0 TH1 1.clean.fq.gz 0:150 1:150 A:1793735270;C:1500551703;G:1518029671;T:1775983548;N:9408 150 150     1793735270 1500551703 1518029671 1775983548 9408 SRX22443197 SRS19460917 SRA1747895 Fujian Agriculture and Forestry University|College of Marine Sciences Fujian Agriculture and Forestry University 2 0.92046 0.91969 0.10181 0.10206 0.61505 0.61477 0.48395 0.48957 150 150 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   China 2023-11-09 Adult Adult Gonad Reproductive System
28811 28811 SRR26744401 SRX22443196 SRS19460916 SRP470937 PRJNA1036532 Danio rerio Raw sequence reads PRJNA1036532 Whole Genome Sequencing normal RNAseq of zebrafish       OL3 AWG21KN135   strain:AB wild type|age:4 mpf|collection date:2021 10 25|geo loc name:China|sex:female|tissue:ovary|BioSampleModel:Model organism or animal                 RNAseq of zebrafish HF3 HF3 normal RNAseq of zebrafish     RNA-Seq TRANSCRIPTOMIC Oligo-dT PAIRED ILLUMINA Illumina HiSeq 4000   SRP470937     OH3_1.clean.fq.gz OH3_2.clean.fq.gz fastq fastq 5690890500.0 18969635.0 OH3 1.clean.fq.gz 0:150 1:150 A:1485585756;C:1351021989;G:1373539521;T:1480733791;N:9443 150 150     1485585756 1351021989 1373539521 1480733791 9443 SRX22443196 SRS19460916 SRA1747895 Fujian Agriculture and Forestry University|College of Marine Sciences Fujian Agriculture and Forestry University 2 0.92616 0.9273 0.01843 0.01865 0.74793 0.74925 0.4724 0.47202 150 150 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   China 2023-11-09 Adult Adult Gonad Reproductive System
28812 28812 SRR26744402 SRX22443195 SRS19460912 SRP470937 PRJNA1036532 Danio rerio Raw sequence reads PRJNA1036532 Whole Genome Sequencing normal RNAseq of zebrafish       OL2 AWG21KN134   strain:AB wild type|age:4 mpf|collection date:2021 10 24|geo loc name:China|sex:female|tissue:ovary|BioSampleModel:Model organism or animal                 RNAseq of zebrafish HF2 HF2 normal RNAseq of zebrafish     RNA-Seq TRANSCRIPTOMIC Oligo-dT PAIRED ILLUMINA Illumina HiSeq 4000   SRP470937     OH2_2.clean.fq.gz OH2_1.clean.fq.gz fastq fastq 6047756700.0 20159189.0 OH2 1.clean.fq.gz 0:150 1:150 A:1577825068;C:1435108510;G:1458998347;T:1575814993;N:9782 150 150     1577825068 1435108510 1458998347 1575814993 9782 SRX22443195 SRS19460912 SRA1747895 Fujian Agriculture and Forestry University|College of Marine Sciences Fujian Agriculture and Forestry University 2 0.9233 0.92363 0.01923 0.01923 0.74474 0.74686 0.46918 0.46911 150 150 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   China 2023-11-09 Adult Adult Gonad Reproductive System
28813 28813 SRR26744403 SRX22443194 SRS19460913 SRP470937 PRJNA1036532 Danio rerio Raw sequence reads PRJNA1036532 Whole Genome Sequencing normal RNAseq of zebrafish       OL1 AWG21KI957   strain:AB wild type|age:4 mpf|collection date:2021 10 23|geo loc name:China|sex:female|tissue:ovary|BioSampleModel:Model organism or animal                 RNAseq of zebrafish HF HF normal RNAseq of zebrafish     RNA-Seq TRANSCRIPTOMIC Oligo-dT PAIRED ILLUMINA Illumina HiSeq 4000   SRP470937     OH1_1.clean.fq.gz OH1_2.clean.fq.gz fastq fastq 5756713800.0 19189046.0 OH1 1.clean.fq.gz 0:150 1:150 A:1501851096;C:1366577079;G:1382910165;T:1505367292;N:8168 150 150     1501851096 1366577079 1382910165 1505367292 8168 SRX22443194 SRS19460913 SRA1747895 Fujian Agriculture and Forestry University|College of Marine Sciences Fujian Agriculture and Forestry University 2 0.92555 0.92628 0.0184 0.01828 0.74968 0.7512 0.47643 0.4776 150 150 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   China 2023-11-09 Adult Adult Gonad Reproductive System
28814 28814 SRR26744404 SRX22443193 SRS19460908 SRP470937 PRJNA1036532 Danio rerio Raw sequence reads PRJNA1036532 Whole Genome Sequencing normal RNAseq of zebrafish       TL3 AWG21KN133   strain:AB wild type|age:4 mpf|collection date:2021 10 22|geo loc name:China|sex:male|tissue:testis|BioSampleModel:Model organism or animal                 RNAseq of zebrafish LM3 LM3 normal RNAseq of zebrafish     RNA-Seq TRANSCRIPTOMIC Oligo-dT PAIRED ILLUMINA Illumina HiSeq 4000   SRP470937     TL3_1.clean.fq.gz TL3_2.clean.fq.gz fastq fastq 5675551800.0 18918506.0 TL3 1.clean.fq.gz 0:150 1:150 A:1544159251;C:1294658828;G:1308831043;T:1527894467;N:8211 150 150     1544159251 1294658828 1308831043 1527894467 8211 SRX22443193 SRS19460908 SRA1747895 Fujian Agriculture and Forestry University|College of Marine Sciences Fujian Agriculture and Forestry University 2 0.92069 0.92047 0.10371 0.10354 0.62741 0.62954 0.48923 0.48839 150 150 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   China 2023-11-09 Adult Adult Gonad Reproductive System
28815 28815 SRR26744405 SRX22443192 SRS19460909 SRP470937 PRJNA1036532 Danio rerio Raw sequence reads PRJNA1036532 Whole Genome Sequencing normal RNAseq of zebrafish       TL2 AWG21KN132   strain:AB wild type|age:4 mpf|collection date:2021 10 21|geo loc name:China|sex:male|tissue:testis|BioSampleModel:Model organism or animal                 RNAseq of zebrafish LM2 LM2 normal RNAseq of zebrafish     RNA-Seq TRANSCRIPTOMIC Oligo-dT PAIRED ILLUMINA Illumina HiSeq 4000   SRP470937     TL2_1.clean.fq.gz TL2_2.clean.fq.gz fastq fastq 5834343300.0 19447811.0 TL2 1.clean.fq.gz 0:150 1:150 A:1583809689;C:1333511774;G:1345919597;T:1571093925;N:8315 150 150     1583809689 1333511774 1345919597 1571093925 8315 SRX22443192 SRS19460909 SRA1747895 Fujian Agriculture and Forestry University|College of Marine Sciences Fujian Agriculture and Forestry University 2 0.91878 0.92054 0.10134 0.10105 0.62708 0.62759 0.48763 0.48708 150 150 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   China 2023-11-09 Adult Adult Gonad Reproductive System
28816 28816 SRR26744406 SRX22443191 SRS19460911 SRP470937 PRJNA1036532 Danio rerio Raw sequence reads PRJNA1036532 Whole Genome Sequencing normal RNAseq of zebrafish       OC2 AWG21KN126   strain:AB wild type|age:4 mpf|collection date:2021 10 12|geo loc name:China|sex:female|tissue:ovary|BioSampleModel:Model organism or animal                 RNAseq of zebrafish CF2 CF2 normal RNAseq of zebrafish     RNA-Seq TRANSCRIPTOMIC Oligo-dT PAIRED ILLUMINA Illumina HiSeq 4000   SRP470937     OC2_2.clean.fq.gz OC2_1.clean.fq.gz fastq fastq 6462335700.0 21541119.0 OC2 1.clean.fq.gz 0:150 1:150 A:1684794140;C:1535771967;G:1553892063;T:1687868172;N:9358 150 150     1684794140 1535771967 1553892063 1687868172 9358 SRX22443191 SRS19460911 SRA1747895 Fujian Agriculture and Forestry University|College of Marine Sciences Fujian Agriculture and Forestry University 2 0.92236 0.92263 0.01803 0.01777 0.74945 0.75081 0.47372 0.47191 150 150 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   China 2023-11-09 Adult Adult Gonad Reproductive System
28817 28817 SRR26744407 SRX22443190 SRS19460907 SRP470937 PRJNA1036532 Danio rerio Raw sequence reads PRJNA1036532 Whole Genome Sequencing normal RNAseq of zebrafish       OC1 AWG21KI953   strain:AB wild type|age:4 mpf|collection date:2021 10 11|geo loc name:China|sex:female|tissue:ovary|BioSampleModel:Model organism or animal                 RNAseq of zebrafish CF CF normal RNAseq of zebrafish     RNA-Seq TRANSCRIPTOMIC Oligo-dT PAIRED ILLUMINA Illumina HiSeq 4000   SRP470937     OC1_1.clean.fq.gz OC1_2.clean.fq.gz fastq fastq 5719061700.0 19063539.0 OC1 1.clean.fq.gz 0:150 1:150 A:1489404392;C:1360123113;G:1386373369;T:1483151708;N:9118 150 150     1489404392 1360123113 1386373369 1483151708 9118 SRX22443190 SRS19460907 SRA1747895 Fujian Agriculture and Forestry University|College of Marine Sciences Fujian Agriculture and Forestry University 2 0.92595 0.92513 0.0166 0.0163 0.75187 0.75503 0.47431 0.47637 150 150 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   China 2023-11-09 Adult Adult Gonad Reproductive System
29259 29259 SRR27336917 SRX23013778 SRS19977075 SRP479907 PRJNA1056454 miR214 regulates sex determination through gsdf in zebrafish PRJNA1056454 Other Sex determination is a variable and complex mechanism yet it can be found all over the plant and animal kingdoms. It creates two morphological different outcomes from one and the same species. Our work demonstrates the involvement of a non coding RNA in this process for the teleost Danio rerio. Although we were not able to understand the full mechanism behind it we can provide a piece of the puzzle which once again highlights the complexity and flexibility of sex determination.     CrisprCas9 generated using 2 sgRNAs >F3 crossed toTgvasa:eGFP miR214 PaternalKO gonads 31dpf rep2 miR214 PaternalKO gonads 31dpf rep2   strain:mz 06de tgvasa:eGFP|isolate:not applicable|breed:TUE|cultivar:not applicable|ecotype:not applicable|age:27 dpf|dev stage:27 dpf|sex:not applicable|tissue:gonads|parental ko:PaternalKO|biological replicate:rep2|number of gonads:9 gonads|birth date:21.09.2018|collection date:not applicable|genotype:homozygous mutation for miR 214 from a heterozygous female crossed to a homozygous male|geo loc name:not applicable|growth protocol:standard|phenotype:n1|sample type:tissue|BioSampleModel:Model organism or animal                 = xlfn.CONCAT"mrna sequencing of zebrafish: " A11 miR214 PaternalKO gonads 31dpf rep2 miR214 PaternalKO gonads 31dpf rep2 NGS library prep was performed with Illumina's TruSeq stranded mRNA LT Sample Prep Kit following Illuminas standard protocol Part # 15031047 Rev E. Libraries were prepared with a starting amount of 193 ng and amplified in 13 PCR cycles Libraries were profiled in a DNA 1000 Chip on a 2100 Bioanalyzer Agilent technologies and quantified using the Qubit dsDNA HS Assay Kit in a Qubit 2 0 Fluorometer Life technologies. All 16 samples were pooled in equimolar ratio and sequenced on 1 NextSeq 500 Highoutput FC SR for 1 x 84 cycles plus 7 cycles for the index read.     RNA-Seq TRANSCRIPTOMIC Oligo-dT SINGLE ILLUMINA NextSeq 500   SRP479907     miR214_PaternalKO_gonads_31dpf_rep2.fastq.gz fastq 2327996832.0 27714248.0 miR214 PaternalKO gonads 31dpf rep2.fastq.gz 0:84 A:602135965;C:552786494;G:537368428;T:635697339;N:8606 84       602135965 552786494 537368428 635697339 8606 SRX23013778 SRS19977075 SRA1775014 Rene Ketting group|Ketting Lab Rene Ketting group 1 0.95369   0.06146   0.69203   0.47259   84   B   usable mapping rate illumina nextseq unknown poly_a trueseq bulk unknown unknown   Germany 2023-12-24 Larval Larval Gonad Reproductive System
29260 29260 SRR27336918 SRX23013777 SRS19977074 SRP479907 PRJNA1056454 miR214 regulates sex determination through gsdf in zebrafish PRJNA1056454 Other Sex determination is a variable and complex mechanism yet it can be found all over the plant and animal kingdoms. It creates two morphological different outcomes from one and the same species. Our work demonstrates the involvement of a non coding RNA in this process for the teleost Danio rerio. Although we were not able to understand the full mechanism behind it we can provide a piece of the puzzle which once again highlights the complexity and flexibility of sex determination.     CrisprCas9 generated using 2 sgRNAs >F3 crossed toTgvasa:eGFP miR214 PaternalKO gonads 31dpf rep1 miR214 PaternalKO gonads 31dpf rep1   strain:mz 06de tgvasa:eGFP|isolate:not applicable|breed:TUE|cultivar:not applicable|ecotype:not applicable|age:27 dpf|dev stage:27 dpf|sex:not applicable|tissue:gonads|parental ko:PaternalKO|biological replicate:rep1|number of gonads:9 gonads|birth date:21.09.2018|collection date:not applicable|genotype:homozygous mutation for miR 214 from a heterozygous female crossed to a homozygous male|geo loc name:not applicable|growth protocol:standard|phenotype:n1|sample type:tissue|BioSampleModel:Model organism or animal                 = xlfn.CONCAT"mrna sequencing of zebrafish: " A10 miR214 PaternalKO gonads 31dpf rep1 miR214 PaternalKO gonads 31dpf rep1 NGS library prep was performed with Illumina's TruSeq stranded mRNA LT Sample Prep Kit following Illuminas standard protocol Part # 15031047 Rev E. Libraries were prepared with a starting amount of 193 ng and amplified in 13 PCR cycles Libraries were profiled in a DNA 1000 Chip on a 2100 Bioanalyzer Agilent technologies and quantified using the Qubit dsDNA HS Assay Kit in a Qubit 2 0 Fluorometer Life technologies. All 16 samples were pooled in equimolar ratio and sequenced on 1 NextSeq 500 Highoutput FC SR for 1 x 84 cycles plus 7 cycles for the index read.     RNA-Seq TRANSCRIPTOMIC Oligo-dT SINGLE ILLUMINA NextSeq 500   SRP479907     miR214_PaternalKO_gonads_31dpf_rep1.fastq.gz fastq 2500978452.0 29773553.0 miR214 PaternalKO gonads 31dpf rep1.fastq.gz 0:84 A:646162933;C:593855971;G:578265372;T:682684551;N:9625 84       646162933 593855971 578265372 682684551 9625 SRX23013777 SRS19977074 SRA1775014 Rene Ketting group|Ketting Lab Rene Ketting group 1 0.95147   0.05972   0.69071   0.47043   84   B   usable mapping rate illumina nextseq unknown poly_a trueseq bulk unknown unknown   Germany 2023-12-24 Larval Larval Gonad Reproductive System
29261 29261 SRR27336919 SRX23013776 SRS19977073 SRP479907 PRJNA1056454 miR214 regulates sex determination through gsdf in zebrafish PRJNA1056454 Other Sex determination is a variable and complex mechanism yet it can be found all over the plant and animal kingdoms. It creates two morphological different outcomes from one and the same species. Our work demonstrates the involvement of a non coding RNA in this process for the teleost Danio rerio. Although we were not able to understand the full mechanism behind it we can provide a piece of the puzzle which once again highlights the complexity and flexibility of sex determination.     CrisprCas9 generated using 2 sgRNAs >F3 crossed toTgvasa:eGFP miR214 maternalHET gonads 31dpf rep4 miR214 maternalHET gonads 31dpf rep4   strain:mz 06de tgvasa:eGFP|isolate:not applicable|breed:TUE|cultivar:not applicable|ecotype:not applicable|age:27 dpf|dev stage:27 dpf|sex:not applicable|tissue:gonads|parental ko:maternalHET|biological replicate:rep4|number of gonads:7 gonads|birth date:21.09.2018|collection date:not applicable|genotype:heterozygous mutation for miR 214 from a homozygous female crossed to a heterozygous male|geo loc name:not applicable|growth protocol:standard|phenotype:n1|sample type:tissue|BioSampleModel:Model organism or animal                 = xlfn.CONCAT"mrna sequencing of zebrafish: " A9 miR214 maternalHET gonads 31dpf rep4 miR214 maternalHET gonads 31dpf rep4 NGS library prep was performed with Illumina's TruSeq stranded mRNA LT Sample Prep Kit following Illuminas standard protocol Part # 15031047 Rev E. Libraries were prepared with a starting amount of 193 ng and amplified in 13 PCR cycles Libraries were profiled in a DNA 1000 Chip on a 2100 Bioanalyzer Agilent technologies and quantified using the Qubit dsDNA HS Assay Kit in a Qubit 2 0 Fluorometer Life technologies. All 16 samples were pooled in equimolar ratio and sequenced on 1 NextSeq 500 Highoutput FC SR for 1 x 84 cycles plus 7 cycles for the index read.     RNA-Seq TRANSCRIPTOMIC Oligo-dT SINGLE ILLUMINA NextSeq 500   SRP479907     miR214_maternalHET_gonads_31dpf_rep4.fastq.gz fastq 2365480908.0 28160487.0 miR214 maternalHET gonads 31dpf rep4.fastq.gz 0:84 A:613121312;C:560582165;G:549338096;T:642429834;N:9501 84       613121312 560582165 549338096 642429834 9501 SRX23013776 SRS19977073 SRA1775014 Rene Ketting group|Ketting Lab Rene Ketting group 1 0.95304   0.05342   0.7108   0.46822   84   B   usable mapping rate illumina nextseq unknown poly_a trueseq bulk unknown unknown   Germany 2023-12-24 Larval Larval Gonad Reproductive System
29262 29262 SRR27336920 SRX23013775 SRS19977072 SRP479907 PRJNA1056454 miR214 regulates sex determination through gsdf in zebrafish PRJNA1056454 Other Sex determination is a variable and complex mechanism yet it can be found all over the plant and animal kingdoms. It creates two morphological different outcomes from one and the same species. Our work demonstrates the involvement of a non coding RNA in this process for the teleost Danio rerio. Although we were not able to understand the full mechanism behind it we can provide a piece of the puzzle which once again highlights the complexity and flexibility of sex determination.     CrisprCas9 generated using 2 sgRNAs >F3 crossed toTgvasa:eGFP miR214 maternalHET gonads 31dpf rep3 miR214 maternalHET gonads 31dpf rep3   strain:mz 06de tgvasa:eGFP|isolate:not applicable|breed:TUE|cultivar:not applicable|ecotype:not applicable|age:27 dpf|dev stage:27 dpf|sex:not applicable|tissue:gonads|parental ko:maternalHET|biological replicate:rep3|number of gonads:7 gonads|birth date:21.09.2018|collection date:not applicable|genotype:heterozygous mutation for miR 214 from a homozygous female crossed to a heterozygous male|geo loc name:not applicable|growth protocol:standard|phenotype:n1|sample type:tissue|BioSampleModel:Model organism or animal                 = xlfn.CONCAT"mrna sequencing of zebrafish: " A8 miR214 maternalHET gonads 31dpf rep3 miR214 maternalHET gonads 31dpf rep3 NGS library prep was performed with Illumina's TruSeq stranded mRNA LT Sample Prep Kit following Illuminas standard protocol Part # 15031047 Rev E. Libraries were prepared with a starting amount of 193 ng and amplified in 13 PCR cycles Libraries were profiled in a DNA 1000 Chip on a 2100 Bioanalyzer Agilent technologies and quantified using the Qubit dsDNA HS Assay Kit in a Qubit 2 0 Fluorometer Life technologies. All 16 samples were pooled in equimolar ratio and sequenced on 1 NextSeq 500 Highoutput FC SR for 1 x 84 cycles plus 7 cycles for the index read.     RNA-Seq TRANSCRIPTOMIC Oligo-dT SINGLE ILLUMINA NextSeq 500   SRP479907     miR214_maternalHET_gonads_31dpf_rep3.fastq.gz fastq 2537794644.0 30211841.0 miR214 maternalHET gonads 31dpf rep3.fastq.gz 0:84 A:658687178;C:600742464;G:585643169;T:692712057;N:9776 84       658687178 600742464 585643169 692712057 9776 SRX23013775 SRS19977072 SRA1775014 Rene Ketting group|Ketting Lab Rene Ketting group 1 0.95177   0.06701   0.68195   0.47393   84   B   usable mapping rate illumina nextseq unknown poly_a trueseq bulk unknown unknown   Germany 2023-12-24 Larval Larval Gonad Reproductive System
29263 29263 SRR27336921 SRX23013774 SRS19977071 SRP479907 PRJNA1056454 miR214 regulates sex determination through gsdf in zebrafish PRJNA1056454 Other Sex determination is a variable and complex mechanism yet it can be found all over the plant and animal kingdoms. It creates two morphological different outcomes from one and the same species. Our work demonstrates the involvement of a non coding RNA in this process for the teleost Danio rerio. Although we were not able to understand the full mechanism behind it we can provide a piece of the puzzle which once again highlights the complexity and flexibility of sex determination.     CrisprCas9 generated using 2 sgRNAs >F3 crossed toTgvasa:eGFP miR214 maternalHET gonads 31dpf rep2 miR214 maternalHET gonads 31dpf rep2   strain:mz 06de tgvasa:eGFP|isolate:not applicable|breed:TUE|cultivar:not applicable|ecotype:not applicable|age:27 dpf|dev stage:27 dpf|sex:not applicable|tissue:gonads|parental ko:maternalHET|biological replicate:rep2|number of gonads:7 gonads|birth date:21.09.2018|collection date:not applicable|genotype:heterozygous mutation for miR 214 from a homozygous female crossed to a heterozygous male|geo loc name:not applicable|growth protocol:standard|phenotype:n1|sample type:tissue|BioSampleModel:Model organism or animal                 = xlfn.CONCAT"mrna sequencing of zebrafish: " A7 miR214 maternalHET gonads 31dpf rep2 miR214 maternalHET gonads 31dpf rep2 NGS library prep was performed with Illumina's TruSeq stranded mRNA LT Sample Prep Kit following Illuminas standard protocol Part # 15031047 Rev E. Libraries were prepared with a starting amount of 193 ng and amplified in 13 PCR cycles Libraries were profiled in a DNA 1000 Chip on a 2100 Bioanalyzer Agilent technologies and quantified using the Qubit dsDNA HS Assay Kit in a Qubit 2 0 Fluorometer Life technologies. All 16 samples were pooled in equimolar ratio and sequenced on 1 NextSeq 500 Highoutput FC SR for 1 x 84 cycles plus 7 cycles for the index read.     RNA-Seq TRANSCRIPTOMIC Oligo-dT SINGLE ILLUMINA NextSeq 500   SRP479907     miR214_maternalHET_gonads_31dpf_rep2.fastq.gz fastq 2417384424.0 28778386.0 miR214 maternalHET gonads 31dpf rep2.fastq.gz 0:84 A:625935186;C:574179061;G:560498085;T:656762980;N:9112 84       625935186 574179061 560498085 656762980 9112 SRX23013774 SRS19977071 SRA1775014 Rene Ketting group|Ketting Lab Rene Ketting group 1 0.95259   0.05852   0.6981   0.47435   84   B   usable mapping rate illumina nextseq unknown poly_a trueseq bulk unknown unknown   Germany 2023-12-24 Larval Larval Gonad Reproductive System
29264 29264 SRR27336922 SRX23013773 SRS19977070 SRP479907 PRJNA1056454 miR214 regulates sex determination through gsdf in zebrafish PRJNA1056454 Other Sex determination is a variable and complex mechanism yet it can be found all over the plant and animal kingdoms. It creates two morphological different outcomes from one and the same species. Our work demonstrates the involvement of a non coding RNA in this process for the teleost Danio rerio. Although we were not able to understand the full mechanism behind it we can provide a piece of the puzzle which once again highlights the complexity and flexibility of sex determination.     CrisprCas9 generated using 2 sgRNAs >F3 crossed toTgvasa:eGFP miR214 maternalHET gonads 31dpf rep1 miR214 maternalHET gonads 31dpf rep1   strain:mz 06de tgvasa:eGFP|isolate:not applicable|breed:TUE|cultivar:not applicable|ecotype:not applicable|age:27 dpf|dev stage:27 dpf|sex:not applicable|tissue:gonads|parental ko:maternalHET|biological replicate:rep1|number of gonads:7 gonads|birth date:21.09.2018|collection date:not applicable|genotype:heterozygous mutation for miR 214 from a homozygous female crossed to a heterozygous male|geo loc name:not applicable|growth protocol:standard|phenotype:n1|sample type:tissue|BioSampleModel:Model organism or animal                 = xlfn.CONCAT"mrna sequencing of zebrafish: " A6 miR214 maternalHET gonads 31dpf rep1 miR214 maternalHET gonads 31dpf rep1 NGS library prep was performed with Illumina's TruSeq stranded mRNA LT Sample Prep Kit following Illuminas standard protocol Part # 15031047 Rev E. Libraries were prepared with a starting amount of 193 ng and amplified in 13 PCR cycles Libraries were profiled in a DNA 1000 Chip on a 2100 Bioanalyzer Agilent technologies and quantified using the Qubit dsDNA HS Assay Kit in a Qubit 2 0 Fluorometer Life technologies. All 16 samples were pooled in equimolar ratio and sequenced on 1 NextSeq 500 Highoutput FC SR for 1 x 84 cycles plus 7 cycles for the index read.     RNA-Seq TRANSCRIPTOMIC Oligo-dT SINGLE ILLUMINA NextSeq 500   SRP479907     miR214_maternalHET_gonads_31dpf_rep1.fastq.gz fastq 2330886180.0 27748645.0 miR214 maternalHET gonads 31dpf rep1.fastq.gz 0:84 A:601589571;C:554105326;G:542789267;T:632393158;N:8858 84       601589571 554105326 542789267 632393158 8858 SRX23013773 SRS19977070 SRA1775014 Rene Ketting group|Ketting Lab Rene Ketting group 1 0.95338   0.05239   0.71366   0.47291   84   B   usable mapping rate illumina nextseq unknown poly_a trueseq bulk unknown unknown   Germany 2023-12-24 Larval Larval Gonad Reproductive System
29265 29265 SRR27336923 SRX23013772 SRS19977069 SRP479907 PRJNA1056454 miR214 regulates sex determination through gsdf in zebrafish PRJNA1056454 Other Sex determination is a variable and complex mechanism yet it can be found all over the plant and animal kingdoms. It creates two morphological different outcomes from one and the same species. Our work demonstrates the involvement of a non coding RNA in this process for the teleost Danio rerio. Although we were not able to understand the full mechanism behind it we can provide a piece of the puzzle which once again highlights the complexity and flexibility of sex determination.     CrisprCas9 generated using 2 sgRNAs >F3 crossed toTgvasa:eGFP miR214 maternalKO gonads 31dpf rep4 miR214 maternalKO gonads 31dpf rep4   strain:mz 06de tgvasa:eGFP|isolate:not applicable|breed:TUE|cultivar:not applicable|ecotype:not applicable|age:27 dpf|dev stage:27 dpf|sex:not applicable|tissue:gonads|parental ko:maternalKO|biological replicate:rep4|number of gonads:8 gonads|birth date:21.09.2018|collection date:not applicable|genotype:homozygous mutation for miR 214 from a homozygous female crossed to a heterozygous male|geo loc name:not applicable|growth protocol:standard|phenotype:n1|sample type:tissue|BioSampleModel:Model organism or animal                 = xlfn.CONCAT"mrna sequencing of zebrafish: " A5 miR214 maternalKO gonads 31dpf rep4 miR214 maternalKO gonads 31dpf rep4 NGS library prep was performed with Illumina's TruSeq stranded mRNA LT Sample Prep Kit following Illuminas standard protocol Part # 15031047 Rev E. Libraries were prepared with a starting amount of 193 ng and amplified in 13 PCR cycles Libraries were profiled in a DNA 1000 Chip on a 2100 Bioanalyzer Agilent technologies and quantified using the Qubit dsDNA HS Assay Kit in a Qubit 2 0 Fluorometer Life technologies. All 16 samples were pooled in equimolar ratio and sequenced on 1 NextSeq 500 Highoutput FC SR for 1 x 84 cycles plus 7 cycles for the index read.     RNA-Seq TRANSCRIPTOMIC Oligo-dT SINGLE ILLUMINA NextSeq 500   SRP479907     miR214_maternalKO_gonads_31dpf_rep4.fastq.gz fastq 2340233784.0 27859926.0 miR214 maternalKO gonads 31dpf rep4.fastq.gz 0:84 A:606735187;C:553647808;G:539865459;T:639976430;N:8900 84       606735187 553647808 539865459 639976430 8900 SRX23013772 SRS19977069 SRA1775014 Rene Ketting group|Ketting Lab Rene Ketting group 1 0.95406   0.06415   0.68225   0.47695   84   B   usable mapping rate illumina nextseq unknown poly_a trueseq bulk unknown unknown   Germany 2023-12-24 Larval Larval Gonad Reproductive System
29266 29266 SRR27336924 SRX23013771 SRS19977068 SRP479907 PRJNA1056454 miR214 regulates sex determination through gsdf in zebrafish PRJNA1056454 Other Sex determination is a variable and complex mechanism yet it can be found all over the plant and animal kingdoms. It creates two morphological different outcomes from one and the same species. Our work demonstrates the involvement of a non coding RNA in this process for the teleost Danio rerio. Although we were not able to understand the full mechanism behind it we can provide a piece of the puzzle which once again highlights the complexity and flexibility of sex determination.     CrisprCas9 generated using 2 sgRNAs >F3 crossed toTgvasa:eGFP miR214 maternalKO gonads 31dpf rep3 miR214 maternalKO gonads 31dpf rep3   strain:mz 06de tgvasa:eGFP|isolate:not applicable|breed:TUE|cultivar:not applicable|ecotype:not applicable|age:27 dpf|dev stage:27 dpf|sex:not applicable|tissue:gonads|parental ko:maternalKO|biological replicate:rep3|number of gonads:9 gonads|birth date:21.09.2018|collection date:not applicable|genotype:homozygous mutation for miR 214 from a homozygous female crossed to a heterozygous male|geo loc name:not applicable|growth protocol:standard|phenotype:n1|sample type:tissue|BioSampleModel:Model organism or animal                 = xlfn.CONCAT"mrna sequencing of zebrafish: " A4 miR214 maternalKO gonads 31dpf rep3 miR214 maternalKO gonads 31dpf rep3 NGS library prep was performed with Illumina's TruSeq stranded mRNA LT Sample Prep Kit following Illuminas standard protocol Part # 15031047 Rev E. Libraries were prepared with a starting amount of 193 ng and amplified in 13 PCR cycles Libraries were profiled in a DNA 1000 Chip on a 2100 Bioanalyzer Agilent technologies and quantified using the Qubit dsDNA HS Assay Kit in a Qubit 2 0 Fluorometer Life technologies. All 16 samples were pooled in equimolar ratio and sequenced on 1 NextSeq 500 Highoutput FC SR for 1 x 84 cycles plus 7 cycles for the index read.     RNA-Seq TRANSCRIPTOMIC Oligo-dT SINGLE ILLUMINA NextSeq 500   SRP479907     miR214_maternalKO_gonads_31dpf_rep3.fastq.gz fastq 2058505512.0 24506018.0 miR214 maternalKO gonads 31dpf rep3.fastq.gz 0:84 A:537425814;C:485664494;G:471526682;T:563881213;N:7309 84       537425814 485664494 471526682 563881213 7309 SRX23013771 SRS19977068 SRA1775014 Rene Ketting group|Ketting Lab Rene Ketting group 1 0.95296   0.06728   0.69266   0.47627   84   B   usable mapping rate illumina nextseq unknown poly_a trueseq bulk unknown unknown   Germany 2023-12-24 Larval Larval Gonad Reproductive System
29267 29267 SRR27336925 SRX23013770 SRS19977067 SRP479907 PRJNA1056454 miR214 regulates sex determination through gsdf in zebrafish PRJNA1056454 Other Sex determination is a variable and complex mechanism yet it can be found all over the plant and animal kingdoms. It creates two morphological different outcomes from one and the same species. Our work demonstrates the involvement of a non coding RNA in this process for the teleost Danio rerio. Although we were not able to understand the full mechanism behind it we can provide a piece of the puzzle which once again highlights the complexity and flexibility of sex determination.     CrisprCas9 generated using 2 sgRNAs >F3 crossed toTgvasa:eGFP miR214 PaternalHET gonads 31dpf rep4 miR214 PaternalHET gonads 31dpf rep4   strain:mz 06de tgvasa:eGFP|isolate:not applicable|breed:TUE|cultivar:not applicable|ecotype:not applicable|age:27 dpf|dev stage:27 dpf|sex:not applicable|tissue:gonads|parental ko:PaternalHET|biological replicate:rep4|number of gonads:9 gonads|birth date:21.09.2018|collection date:not applicable|genotype:heterozygous mutation for miR 214 from a heterozygous female crossed to a homozygous male|geo loc name:not applicable|growth protocol:standard|phenotype:n1|sample type:tissue|BioSampleModel:Model organism or animal                 = xlfn.CONCAT"mrna sequencing of zebrafish: " A17 miR214 PaternalHET gonads 31dpf rep4 miR214 PaternalHET gonads 31dpf rep4 NGS library prep was performed with Illumina's TruSeq stranded mRNA LT Sample Prep Kit following Illuminas standard protocol Part # 15031047 Rev E. Libraries were prepared with a starting amount of 193 ng and amplified in 13 PCR cycles Libraries were profiled in a DNA 1000 Chip on a 2100 Bioanalyzer Agilent technologies and quantified using the Qubit dsDNA HS Assay Kit in a Qubit 2 0 Fluorometer Life technologies. All 16 samples were pooled in equimolar ratio and sequenced on 1 NextSeq 500 Highoutput FC SR for 1 x 84 cycles plus 7 cycles for the index read.     RNA-Seq TRANSCRIPTOMIC Oligo-dT SINGLE ILLUMINA NextSeq 500   SRP479907     miR214_PaternalHET_gonads_31dpf_rep4.fastq.gz fastq 3001613328.0 35733492.0 miR214 PaternalHET gonads 31dpf rep4.fastq.gz 0:84 A:778860501;C:711155422;G:688784969;T:822801157;N:11279 84       778860501 711155422 688784969 822801157 11279 SRX23013770 SRS19977067 SRA1775014 Rene Ketting group|Ketting Lab Rene Ketting group 1 0.95361   0.05653   0.7051   0.48382   84   B   usable mapping rate illumina nextseq unknown poly_a trueseq bulk unknown unknown   Germany 2023-12-24 Larval Larval Gonad Reproductive System
29268 29268 SRR27336926 SRX23013769 SRS19977066 SRP479907 PRJNA1056454 miR214 regulates sex determination through gsdf in zebrafish PRJNA1056454 Other Sex determination is a variable and complex mechanism yet it can be found all over the plant and animal kingdoms. It creates two morphological different outcomes from one and the same species. Our work demonstrates the involvement of a non coding RNA in this process for the teleost Danio rerio. Although we were not able to understand the full mechanism behind it we can provide a piece of the puzzle which once again highlights the complexity and flexibility of sex determination.     CrisprCas9 generated using 2 sgRNAs >F3 crossed toTgvasa:eGFP miR214 PaternalHET gonads 31dpf rep3 miR214 PaternalHET gonads 31dpf rep3   strain:mz 06de tgvasa:eGFP|isolate:not applicable|breed:TUE|cultivar:not applicable|ecotype:not applicable|age:27 dpf|dev stage:27 dpf|sex:not applicable|tissue:gonads|parental ko:PaternalHET|biological replicate:rep3|number of gonads:9 gonads|birth date:21.09.2018|collection date:not applicable|genotype:heterozygous mutation for miR 214 from a heterozygous female crossed to a homozygous male|geo loc name:not applicable|growth protocol:standard|phenotype:n1|sample type:tissue|BioSampleModel:Model organism or animal                 = xlfn.CONCAT"mrna sequencing of zebrafish: " A16 miR214 PaternalHET gonads 31dpf rep3 miR214 PaternalHET gonads 31dpf rep3 NGS library prep was performed with Illumina's TruSeq stranded mRNA LT Sample Prep Kit following Illuminas standard protocol Part # 15031047 Rev E. Libraries were prepared with a starting amount of 193 ng and amplified in 13 PCR cycles Libraries were profiled in a DNA 1000 Chip on a 2100 Bioanalyzer Agilent technologies and quantified using the Qubit dsDNA HS Assay Kit in a Qubit 2 0 Fluorometer Life technologies. All 16 samples were pooled in equimolar ratio and sequenced on 1 NextSeq 500 Highoutput FC SR for 1 x 84 cycles plus 7 cycles for the index read.     RNA-Seq TRANSCRIPTOMIC Oligo-dT SINGLE ILLUMINA NextSeq 500   SRP479907     miR214_PaternalHET_gonads_31dpf_rep3.fastq.gz fastq 3053630664.0 36352746.0 miR214 PaternalHET gonads 31dpf rep3.fastq.gz 0:84 A:785219727;C:726431662;G:713454025;T:828513556;N:11694 84       785219727 726431662 713454025 828513556 11694 SRX23013769 SRS19977066 SRA1775014 Rene Ketting group|Ketting Lab Rene Ketting group 1 0.95309   0.04588   0.71997   0.46447   84   B   usable mapping rate illumina nextseq unknown poly_a trueseq bulk unknown unknown   Germany 2023-12-24 Larval Larval Gonad Reproductive System
29269 29269 SRR27336927 SRX23013768 SRS19977065 SRP479907 PRJNA1056454 miR214 regulates sex determination through gsdf in zebrafish PRJNA1056454 Other Sex determination is a variable and complex mechanism yet it can be found all over the plant and animal kingdoms. It creates two morphological different outcomes from one and the same species. Our work demonstrates the involvement of a non coding RNA in this process for the teleost Danio rerio. Although we were not able to understand the full mechanism behind it we can provide a piece of the puzzle which once again highlights the complexity and flexibility of sex determination.     CrisprCas9 generated using 2 sgRNAs >F3 crossed toTgvasa:eGFP miR214 PaternalHET gonads 31dpf rep2 miR214 PaternalHET gonads 31dpf rep2   strain:mz 06de tgvasa:eGFP|isolate:not applicable|breed:TUE|cultivar:not applicable|ecotype:not applicable|age:27 dpf|dev stage:27 dpf|sex:not applicable|tissue:gonads|parental ko:PaternalHET|biological replicate:rep2|number of gonads:9 gonads|birth date:21.09.2018|collection date:not applicable|genotype:heterozygous mutation for miR 214 from a heterozygous female crossed to a homozygous male|geo loc name:not applicable|growth protocol:standard|phenotype:n1|sample type:tissue|BioSampleModel:Model organism or animal                 = xlfn.CONCAT"mrna sequencing of zebrafish: " A15 miR214 PaternalHET gonads 31dpf rep2 miR214 PaternalHET gonads 31dpf rep2 NGS library prep was performed with Illumina's TruSeq stranded mRNA LT Sample Prep Kit following Illuminas standard protocol Part # 15031047 Rev E. Libraries were prepared with a starting amount of 193 ng and amplified in 13 PCR cycles Libraries were profiled in a DNA 1000 Chip on a 2100 Bioanalyzer Agilent technologies and quantified using the Qubit dsDNA HS Assay Kit in a Qubit 2 0 Fluorometer Life technologies. All 16 samples were pooled in equimolar ratio and sequenced on 1 NextSeq 500 Highoutput FC SR for 1 x 84 cycles plus 7 cycles for the index read.     RNA-Seq TRANSCRIPTOMIC Oligo-dT SINGLE ILLUMINA NextSeq 500   SRP479907     miR214_PaternalHET_gonads_31dpf_rep2.fastq.gz fastq 3063695208.0 36472562.0 miR214 PaternalHET gonads 31dpf rep2.fastq.gz 0:84 A:784924646;C:728941687;G:720847462;T:828969510;N:11903 84       784924646 728941687 720847462 828969510 11903 SRX23013768 SRS19977065 SRA1775014 Rene Ketting group|Ketting Lab Rene Ketting group 1 0.95413   0.04739   0.71936   0.4704   84   B   usable mapping rate illumina nextseq unknown poly_a trueseq bulk unknown unknown   Germany 2023-12-24 Larval Larval Gonad Reproductive System
29270 29270 SRR27336928 SRX23013767 SRS19977064 SRP479907 PRJNA1056454 miR214 regulates sex determination through gsdf in zebrafish PRJNA1056454 Other Sex determination is a variable and complex mechanism yet it can be found all over the plant and animal kingdoms. It creates two morphological different outcomes from one and the same species. Our work demonstrates the involvement of a non coding RNA in this process for the teleost Danio rerio. Although we were not able to understand the full mechanism behind it we can provide a piece of the puzzle which once again highlights the complexity and flexibility of sex determination.     CrisprCas9 generated using 2 sgRNAs >F3 crossed toTgvasa:eGFP miR214 PaternalHET gonads 31dpf rep1 miR214 PaternalHET gonads 31dpf rep1   strain:mz 06de tgvasa:eGFP|isolate:not applicable|breed:TUE|cultivar:not applicable|ecotype:not applicable|age:27 dpf|dev stage:27 dpf|sex:not applicable|tissue:gonads|parental ko:PaternalHET|biological replicate:rep1|number of gonads:9 gonads|birth date:21.09.2018|collection date:not applicable|genotype:heterozygous mutation for miR 214 from a heterozygous female crossed to a homozygous male|geo loc name:not applicable|growth protocol:standard|phenotype:n1|sample type:tissue|BioSampleModel:Model organism or animal                 = xlfn.CONCAT"mrna sequencing of zebrafish: " A14 miR214 PaternalHET gonads 31dpf rep1 miR214 PaternalHET gonads 31dpf rep1 NGS library prep was performed with Illumina's TruSeq stranded mRNA LT Sample Prep Kit following Illuminas standard protocol Part # 15031047 Rev E. Libraries were prepared with a starting amount of 193 ng and amplified in 13 PCR cycles Libraries were profiled in a DNA 1000 Chip on a 2100 Bioanalyzer Agilent technologies and quantified using the Qubit dsDNA HS Assay Kit in a Qubit 2 0 Fluorometer Life technologies. All 16 samples were pooled in equimolar ratio and sequenced on 1 NextSeq 500 Highoutput FC SR for 1 x 84 cycles plus 7 cycles for the index read.     RNA-Seq TRANSCRIPTOMIC Oligo-dT SINGLE ILLUMINA NextSeq 500   SRP479907     miR214_PaternalHET_gonads_31dpf_rep1.fastq.gz fastq 2699154024.0 32132786.0 miR214 PaternalHET gonads 31dpf rep1.fastq.gz 0:84 A:694174797;C:642049572;G:627991562;T:734927762;N:10331 84       694174797 642049572 627991562 734927762 10331 SRX23013767 SRS19977064 SRA1775014 Rene Ketting group|Ketting Lab Rene Ketting group 1 0.95518   0.05525   0.70285   0.47695   84   B   usable mapping rate illumina nextseq unknown poly_a trueseq bulk unknown unknown   Germany 2023-12-24 Larval Larval Gonad Reproductive System
29271 29271 SRR27336929 SRX23013766 SRS19977063 SRP479907 PRJNA1056454 miR214 regulates sex determination through gsdf in zebrafish PRJNA1056454 Other Sex determination is a variable and complex mechanism yet it can be found all over the plant and animal kingdoms. It creates two morphological different outcomes from one and the same species. Our work demonstrates the involvement of a non coding RNA in this process for the teleost Danio rerio. Although we were not able to understand the full mechanism behind it we can provide a piece of the puzzle which once again highlights the complexity and flexibility of sex determination.     CrisprCas9 generated using 2 sgRNAs >F3 crossed toTgvasa:eGFP miR214 PaternalKO gonads 31dpf rep4 miR214 PaternalKO gonads 31dpf rep4   strain:mz 06de tgvasa:eGFP|isolate:not applicable|breed:TUE|cultivar:not applicable|ecotype:not applicable|age:27 dpf|dev stage:27 dpf|sex:not applicable|tissue:gonads|parental ko:PaternalKO|biological replicate:rep4|number of gonads:8 gonads|birth date:21.09.2018|collection date:not applicable|genotype:homozygous mutation for miR 214 from a heterozygous female crossed to a homozygous male|geo loc name:not applicable|growth protocol:standard|phenotype:n1|sample type:tissue|BioSampleModel:Model organism or animal                 = xlfn.CONCAT"mrna sequencing of zebrafish: " A13 miR214 PaternalKO gonads 31dpf rep4 miR214 PaternalKO gonads 31dpf rep4 NGS library prep was performed with Illumina's TruSeq stranded mRNA LT Sample Prep Kit following Illuminas standard protocol Part # 15031047 Rev E. Libraries were prepared with a starting amount of 193 ng and amplified in 13 PCR cycles Libraries were profiled in a DNA 1000 Chip on a 2100 Bioanalyzer Agilent technologies and quantified using the Qubit dsDNA HS Assay Kit in a Qubit 2 0 Fluorometer Life technologies. All 16 samples were pooled in equimolar ratio and sequenced on 1 NextSeq 500 Highoutput FC SR for 1 x 84 cycles plus 7 cycles for the index read.     RNA-Seq TRANSCRIPTOMIC Oligo-dT SINGLE ILLUMINA NextSeq 500   SRP479907     miR214_PaternalKO_gonads_31dpf_rep4.fastq.gz fastq 2514248016.0 29931524.0 miR214 PaternalKO gonads 31dpf rep4.fastq.gz 0:84 A:648908956;C:598837726;G:580002743;T:686488678;N:9913 84       648908956 598837726 580002743 686488678 9913 SRX23013766 SRS19977063 SRA1775014 Rene Ketting group|Ketting Lab Rene Ketting group 1 0.94978   0.06951   0.67896   0.47686   84   B   usable mapping rate illumina nextseq unknown poly_a trueseq bulk unknown unknown   Germany 2023-12-24 Larval Larval Gonad Reproductive System
29272 29272 SRR27336930 SRX23013765 SRS19977062 SRP479907 PRJNA1056454 miR214 regulates sex determination through gsdf in zebrafish PRJNA1056454 Other Sex determination is a variable and complex mechanism yet it can be found all over the plant and animal kingdoms. It creates two morphological different outcomes from one and the same species. Our work demonstrates the involvement of a non coding RNA in this process for the teleost Danio rerio. Although we were not able to understand the full mechanism behind it we can provide a piece of the puzzle which once again highlights the complexity and flexibility of sex determination.     CrisprCas9 generated using 2 sgRNAs >F3 crossed toTgvasa:eGFP miR214 PaternalKO gonads 31dpf rep3 miR214 PaternalKO gonads 31dpf rep3   strain:mz 06de tgvasa:eGFP|isolate:not applicable|breed:TUE|cultivar:not applicable|ecotype:not applicable|age:27 dpf|dev stage:27 dpf|sex:not applicable|tissue:gonads|parental ko:PaternalKO|biological replicate:rep3|number of gonads:9 gonads|birth date:21.09.2018|collection date:not applicable|genotype:homozygous mutation for miR 214 from a heterozygous female crossed to a homozygous male|geo loc name:not applicable|growth protocol:standard|phenotype:n1|sample type:tissue|BioSampleModel:Model organism or animal                 = xlfn.CONCAT"mrna sequencing of zebrafish: " A12 miR214 PaternalKO gonads 31dpf rep3 miR214 PaternalKO gonads 31dpf rep3 NGS library prep was performed with Illumina's TruSeq stranded mRNA LT Sample Prep Kit following Illuminas standard protocol Part # 15031047 Rev E. Libraries were prepared with a starting amount of 193 ng and amplified in 13 PCR cycles Libraries were profiled in a DNA 1000 Chip on a 2100 Bioanalyzer Agilent technologies and quantified using the Qubit dsDNA HS Assay Kit in a Qubit 2 0 Fluorometer Life technologies. All 16 samples were pooled in equimolar ratio and sequenced on 1 NextSeq 500 Highoutput FC SR for 1 x 84 cycles plus 7 cycles for the index read.     RNA-Seq TRANSCRIPTOMIC Oligo-dT SINGLE ILLUMINA NextSeq 500   SRP479907     miR214_PaternalKO_gonads_31dpf_rep3.fastq.gz fastq 2499529200.0 29756300.0 miR214 PaternalKO gonads 31dpf rep3.fastq.gz 0:84 A:642490769;C:597403823;G:580565141;T:679060154;N:9313 84       642490769 597403823 580565141 679060154 9313 SRX23013765 SRS19977062 SRA1775014 Rene Ketting group|Ketting Lab Rene Ketting group 1 0.95284   0.05602   0.70289   0.47154   84   B   usable mapping rate illumina nextseq unknown poly_a trueseq bulk unknown unknown   Germany 2023-12-24 Larval Larval Gonad Reproductive System
29273 29273 SRR27336931 SRX23013764 SRS19977061 SRP479907 PRJNA1056454 miR214 regulates sex determination through gsdf in zebrafish PRJNA1056454 Other Sex determination is a variable and complex mechanism yet it can be found all over the plant and animal kingdoms. It creates two morphological different outcomes from one and the same species. Our work demonstrates the involvement of a non coding RNA in this process for the teleost Danio rerio. Although we were not able to understand the full mechanism behind it we can provide a piece of the puzzle which once again highlights the complexity and flexibility of sex determination.     CrisprCas9 generated using 2 sgRNAs >F3 crossed toTgvasa:eGFP miR214 maternalKO gonads 31dpf rep2 miR214 maternalKO gonads 31dpf rep2   strain:mz 06de tgvasa:eGFP|isolate:not applicable|breed:TUE|cultivar:not applicable|ecotype:not applicable|age:27 dpf|dev stage:27 dpf|sex:not applicable|tissue:gonads|parental ko:maternalKO|biological replicate:rep2|number of gonads:9 gonads|birth date:21.09.2018|collection date:not applicable|genotype:homozygous mutation for miR 214 from a homozygous female crossed to a heterozygous male|geo loc name:not applicable|growth protocol:standard|phenotype:n1|sample type:tissue|BioSampleModel:Model organism or animal                 = xlfn.CONCAT"mrna sequencing of zebrafish: " A3 miR214 maternalKO gonads 31dpf rep2 miR214 maternalKO gonads 31dpf rep2 NGS library prep was performed with Illumina's TruSeq stranded mRNA LT Sample Prep Kit following Illuminas standard protocol Part # 15031047 Rev E. Libraries were prepared with a starting amount of 193 ng and amplified in 13 PCR cycles Libraries were profiled in a DNA 1000 Chip on a 2100 Bioanalyzer Agilent technologies and quantified using the Qubit dsDNA HS Assay Kit in a Qubit 2 0 Fluorometer Life technologies. All 16 samples were pooled in equimolar ratio and sequenced on 1 NextSeq 500 Highoutput FC SR for 1 x 84 cycles plus 7 cycles for the index read.     RNA-Seq TRANSCRIPTOMIC Oligo-dT SINGLE ILLUMINA NextSeq 500   SRP479907     miR214_maternalKO_gonads_31dpf_rep2.fastq.gz fastq 2529742404.0 30115981.0 miR214 maternalKO gonads 31dpf rep2.fastq.gz 0:84 A:652091166;C:600932059;G:589100617;T:687608862;N:9700 84       652091166 600932059 589100617 687608862 9700 SRX23013764 SRS19977061 SRA1775014 Rene Ketting group|Ketting Lab Rene Ketting group 1 0.95498   0.05524   0.70366   0.47319   84   B   usable mapping rate illumina nextseq unknown poly_a trueseq bulk unknown unknown   Germany 2023-12-24 Larval Larval Gonad Reproductive System
29274 29274 SRR27336932 SRX23013763 SRS19977060 SRP479907 PRJNA1056454 miR214 regulates sex determination through gsdf in zebrafish PRJNA1056454 Other Sex determination is a variable and complex mechanism yet it can be found all over the plant and animal kingdoms. It creates two morphological different outcomes from one and the same species. Our work demonstrates the involvement of a non coding RNA in this process for the teleost Danio rerio. Although we were not able to understand the full mechanism behind it we can provide a piece of the puzzle which once again highlights the complexity and flexibility of sex determination.     CrisprCas9 generated using 2 sgRNAs >F3 crossed toTgvasa:eGFP miR214 maternalKO gonads 31dpf rep1 miR214 maternalKO gonads 31dpf rep1   strain:mz 06de tgvasa:eGFP|isolate:not applicable|breed:TUE|cultivar:not applicable|ecotype:not applicable|age:27 dpf|dev stage:27 dpf|sex:not applicable|tissue:gonads|parental ko:maternalKO|biological replicate:rep1|number of gonads:9 gonads|birth date:21.09.2018|collection date:not applicable|genotype:homozygous mutation for miR 214 from a homozygous female crossed to a heterozygous male|geo loc name:not applicable|growth protocol:standard|phenotype:n1|sample type:tissue|BioSampleModel:Model organism or animal                 = xlfn.CONCAT"mrna sequencing of zebrafish: " A2 miR214 maternalKO gonads 31dpf rep1 miR214 maternalKO gonads 31dpf rep1 NGS library prep was performed with Illumina's TruSeq stranded mRNA LT Sample Prep Kit following Illuminas standard protocol Part # 15031047 Rev E. Libraries were prepared with a starting amount of 193 ng and amplified in 13 PCR cycles Libraries were profiled in a DNA 1000 Chip on a 2100 Bioanalyzer Agilent technologies and quantified using the Qubit dsDNA HS Assay Kit in a Qubit 2 0 Fluorometer Life technologies. All 16 samples were pooled in equimolar ratio and sequenced on 1 NextSeq 500 Highoutput FC SR for 1 x 84 cycles plus 7 cycles for the index read.     RNA-Seq TRANSCRIPTOMIC Oligo-dT SINGLE ILLUMINA NextSeq 500   SRP479907     miR214_maternalKO_gonads_31dpf_rep1.fastq.gz fastq 2310878136.0 27510454.0 miR214 maternalKO gonads 31dpf rep1.fastq.gz 0:84 A:598541924;C:547875391;G:533137786;T:631314274;N:8761 84       598541924 547875391 533137786 631314274 8761 SRX23013763 SRS19977060 SRA1775014 Rene Ketting group|Ketting Lab Rene Ketting group 1 0.95279   0.06446   0.68757   0.4758   84   B   usable mapping rate illumina nextseq unknown poly_a trueseq bulk unknown unknown   Germany 2023-12-24 Larval Larval Gonad Reproductive System
29720 29720 SRR27485665 SRX23156884 SRS20107305 SRP482074 PRJNA1061456 tRAM seq: tRNA abundance and modification analysis during zebrafish embryo development PRJNA1061456 Other           ovary R3   strain:TLAB fish|isolate:NA|breed:cross of zebrafish AB and the natural variant TL Tupfel Longfin|cultivar:NA|ecotype:NA|dev stage:adult|collection date:2022|geo loc name:Austria|sex:female|tissue:ovary|replicate:3|BioSampleModel:Model organism or animal                 mRNA seq of zebrafish: ovary rep4 EV06008 EV06008 RNA was extracted with Trizol and processed with QuantSeq three prime mRNA Seq Library Prep Kit FWD for Illumina Lexogen. 500 ng total RNA per sample. Single indexed QuantSeq libraries were QC checked on a Bioanalyzer 2100 Agilent using a High Sensitivity DNA Kit for correct insert size and quantified using Qubit dsDNA HS Assay Invitrogen. Pooled libraries were sequenced on a NextSeq500 instrument Illumina in 1x75bp single end sequencing mode     RNA-Seq TRANSCRIPTOMIC Oligo-dT SINGLE ILLUMINA NextSeq 500   SRP482074     EV06008.R1.fastq.gz fastq 585955025.0 7769646.0 EV06008.R1.fastq.gz 0:75.42 A:167210227;C:106735220;G:128095616;T:183896888;N:17074 75       167210227 106735220 128095616 183896888 17074 SRX23156884 SRS20107305 SRA1783314 Medical University of Vienna|Cell and Developmental Biology Medical University of Vienna 1 0.85544   0.05096   0.78441   0.53007   76   B   usable mapping rate illumina nextseq 3prime poly_a lexogen bulk unknown unknown   Austria 2024-01-11 Adult Adult Gonad Reproductive System
29731 29731 SRR27485676 SRX23156873 SRS20107294 SRP482074 PRJNA1061456 tRAM seq: tRNA abundance and modification analysis during zebrafish embryo development PRJNA1061456 Other           ovary R2   strain:TLAB fish|isolate:NA|breed:cross of zebrafish AB and the natural variant TL Tupfel Longfin|cultivar:NA|ecotype:NA|dev stage:adult|collection date:2022|geo loc name:Austria|sex:female|tissue:ovary|replicate:2|BioSampleModel:Model organism or animal                 mRNA seq of zebrafish: ovary rep4 EV06001 EV06001 RNA was extracted with Trizol and processed with QuantSeq three prime mRNA Seq Library Prep Kit FWD for Illumina Lexogen. 500 ng total RNA per sample. Single indexed QuantSeq libraries were QC checked on a Bioanalyzer 2100 Agilent using a High Sensitivity DNA Kit for correct insert size and quantified using Qubit dsDNA HS Assay Invitrogen. Pooled libraries were sequenced on a NextSeq500 instrument Illumina in 1x75bp single end sequencing mode     RNA-Seq TRANSCRIPTOMIC Oligo-dT SINGLE ILLUMINA NextSeq 500   SRP482074     EV06001.R1.fastq.gz fastq 685280356.0 9098789.0 EV06001.R1.fastq.gz 0:75.32 A:194596366;C:122550539;G:153504013;T:214558450;N:70988 75       194596366 122550539 153504013 214558450 70988 SRX23156873 SRS20107294 SRA1783314 Medical University of Vienna|Cell and Developmental Biology Medical University of Vienna 1 0.8304   0.04498   0.80166   0.5028   74   B   usable mapping rate illumina nextseq 3prime poly_a lexogen bulk unknown unknown   Austria 2024-01-11 Adult Adult Gonad Reproductive System
29738 29738 SRR27477298 SRX23148649 SRS20099367 SRP482074 PRJNA1061456 tRAM seq: tRNA abundance and modification analysis during zebrafish embryo development PRJNA1061456 Other           ovary R4   strain:TLAB fish|isolate:NA|breed:cross of zebrafish AB and the natural variant TL Tupfel Longfin|cultivar:NA|ecotype:NA|dev stage:adult|collection date:2022|geo loc name:Austria|sex:female|tissue:ovary|replicate:4|BioSampleModel:Model organism or animal                 mRNA seq of zebrafish: ovary rep4 EV09001 EV09001 RNA was extracted with Trizol and processed with QuantSeq three prime mRNA Seq Library Prep Kit FWD for Illumina Lexogen. 500 ng total RNA per sample. Single indexed QuantSeq libraries were QC checked on a Bioanalyzer 2100 Agilent using a High Sensitivity DNA Kit for correct insert size and quantified using Qubit dsDNA HS Assay Invitrogen. Pooled libraries were sequenced on a NextSeq500 instrument Illumina in 1x75bp single end sequencing mode     RNA-Seq TRANSCRIPTOMIC Oligo-dT SINGLE ILLUMINA NextSeq 500   SRP482074     EV09001.R1.fastq.gz fastq 456295310.0 6058854.0 EV09001.R1.fastq.gz 0:75.31 A:135579999;C:83364677;G:101608151;T:135700843;N:41640 75       135579999 83364677 101608151 135700843 41640 SRX23148649 SRS20099367 SRA1782413 Medical University of Vienna|Cell and Developmental Biology Medical University of Vienna 1 0.85088   0.05876   0.7791   0.50948   75   B   usable mapping rate illumina nextseq 3prime poly_a lexogen bulk unknown unknown   Austria 2024-01-10 Adult Adult Gonad Reproductive System
35796 35796 SRR33037156 SRX28302718 SRS24645585 SRP577302 PRJNA1247234 Danio rerio Raw sequence reads PRJNA1247234 Whole Genome Sequencing normal RNA sequencing of gonads in Zebrafish Danio rerio         ZM High 1   breed:zebrafish|age:adult|collection date:2022 01 05|geo loc name:not collected|sex:male|tissue:gonad|replicate:replicate=biological replicate 10|BioSampleModel:Model organism or animal                 RNA seq of zebrafish zebra10 zebra10 normal RNA seq of zebrafish     RNA-Seq TRANSCRIPTOMIC Oligo-dT PAIRED ILLUMINA Illumina NovaSeq X Plus   SRP577302     L1EGC150013--ZM_H1.R1.raw.fastq.gz L1EGC150013--ZM_H1.R2.raw.fastq.gz fastq fastq 13766750030.0 45585265.0 L1EGC150013 ZM H1.R1.raw.fastq.gz 0:151 1:151 A:3583993287;C:3270287453;G:3445394564;T:3467034939;N:39787 151 151     3583993287 3270287453 3445394564 3467034939 39787 SRX28302718 SRS24645585 SRA2108762 South China Normal University|School of environment South China Normal University                       B B biological fallback assumption illumina novaseq_era unknown poly_a unknown bulk unknown unknown   China 2025-04-09 Adult Adult Gonad Reproductive System
35797 35797 SRR33037157 SRX28302717 SRS24645584 SRP577302 PRJNA1247234 Danio rerio Raw sequence reads PRJNA1247234 Whole Genome Sequencing normal RNA sequencing of gonads in Zebrafish Danio rerio         ZM Ctrl 3   breed:zebrafish|age:adult|collection date:2022 01 05|geo loc name:not collected|sex:male|tissue:gonad|replicate:replicate=biological replicate 9|BioSampleModel:Model organism or animal                 RNA seq of zebrafish zebra9 zebra9 normal RNA seq of zebrafish     RNA-Seq TRANSCRIPTOMIC Oligo-dT PAIRED ILLUMINA Illumina NovaSeq X Plus   SRP577302     L1EGC150012--ZM_C3.R1.raw.fastq.gz L1EGC150012--ZM_C3.R2.raw.fastq.gz fastq fastq 13755826086.0 45549093.0 L1EGC150012 ZM C3.R1.raw.fastq.gz 0:151 1:151 A:3584761676;C:3253145488;G:3462274730;T:3455603950;N:40242 151 151     3584761676 3253145488 3462274730 3455603950 40242 SRX28302717 SRS24645584 SRA2108762 South China Normal University|School of environment South China Normal University                       B B biological fallback assumption illumina novaseq_era unknown poly_a unknown bulk unknown unknown   China 2025-04-09 Adult Adult Gonad Reproductive System
35798 35798 SRR33037158 SRX28302716 SRS24645583 SRP577302 PRJNA1247234 Danio rerio Raw sequence reads PRJNA1247234 Whole Genome Sequencing normal RNA sequencing of gonads in Zebrafish Danio rerio         ZM Ctrl 2   breed:zebrafish|age:adult|collection date:2022 01 05|geo loc name:not collected|sex:male|tissue:gonad|replicate:replicate=biological replicate 8|BioSampleModel:Model organism or animal                 RNA seq of zebrafish zebra8 zebra8 normal RNA seq of zebrafish     RNA-Seq TRANSCRIPTOMIC Oligo-dT PAIRED ILLUMINA Illumina NovaSeq X Plus   SRP577302     L1EGC150011--ZM_C2.R1.raw.fastq.gz L1EGC150011--ZM_C2.R2.raw.fastq.gz fastq fastq 13785416952.0 45647076.0 L1EGC150011 ZM C2.R1.raw.fastq.gz 0:151 1:151 A:3612145557;C:3259005821;G:3452125827;T:3462099514;N:40233 151 151     3612145557 3259005821 3452125827 3462099514 40233 SRX28302716 SRS24645583 SRA2108762 South China Normal University|School of environment South China Normal University                       B B biological fallback assumption illumina novaseq_era unknown poly_a unknown bulk unknown unknown   China 2025-04-09 Adult Adult Gonad Reproductive System
35799 35799 SRR33037159 SRX28302715 SRS24645582 SRP577302 PRJNA1247234 Danio rerio Raw sequence reads PRJNA1247234 Whole Genome Sequencing normal RNA sequencing of gonads in Zebrafish Danio rerio         ZM Ctrl 1   breed:zebrafish|age:adult|collection date:2022 01 05|geo loc name:not collected|sex:male|tissue:gonad|replicate:replicate=biological replicate 7|BioSampleModel:Model organism or animal                 RNA seq of zebrafish zebra7 zebra7 normal RNA seq of zebrafish     RNA-Seq TRANSCRIPTOMIC Oligo-dT PAIRED ILLUMINA Illumina NovaSeq X Plus   SRP577302     L1EGC150010--ZM_C1.R1.raw.fastq.gz L1EGC150010--ZM_C1.R2.raw.fastq.gz fastq fastq 14121084214.0 46758557.0 L1EGC150010 ZM C1.R1.raw.fastq.gz 0:151 1:151 A:3692928602;C:3331119636;G:3548101869;T:3548892874;N:41233 151 151     3692928602 3331119636 3548101869 3548892874 41233 SRX28302715 SRS24645582 SRA2108762 South China Normal University|School of environment South China Normal University                       B B biological fallback assumption illumina novaseq_era unknown poly_a unknown bulk unknown unknown   China 2025-04-09 Adult Adult Gonad Reproductive System
35800 35800 SRR33037160 SRX28302714 SRS24645581 SRP577302 PRJNA1247234 Danio rerio Raw sequence reads PRJNA1247234 Whole Genome Sequencing normal RNA sequencing of gonads in Zebrafish Danio rerio         ZF High 3   breed:zebrafish|age:adult|collection date:2022 01 05|geo loc name:not collected|sex:female|tissue:gonad|replicate:replicate=biological replicate 6|BioSampleModel:Model organism or animal                 RNA seq of zebrafish zebra6 zebra6 normal RNA seq of zebrafish     RNA-Seq TRANSCRIPTOMIC Oligo-dT PAIRED ILLUMINA Illumina NovaSeq X Plus   SRP577302     L1EGC150009--ZF_H3.R1.raw.fastq.gz L1EGC150009--ZF_H3.R2.raw.fastq.gz fastq fastq 12796935920.0 42373960.0 L1EGC150009 ZF H3.R1.raw.fastq.gz 0:151 1:151 A:3264528838;C:3096858573;G:3284485238;T:3151025993;N:37278 151 151     3264528838 3096858573 3284485238 3151025993 37278 SRX28302714 SRS24645581 SRA2108762 South China Normal University|School of environment South China Normal University                       B B biological fallback assumption illumina novaseq_era unknown poly_a unknown bulk unknown unknown   China 2025-04-09 Adult Adult Gonad Reproductive System
35801 35801 SRR33037161 SRX28302713 SRS24645580 SRP577302 PRJNA1247234 Danio rerio Raw sequence reads PRJNA1247234 Whole Genome Sequencing normal RNA sequencing of gonads in Zebrafish Danio rerio         ZF High 2   breed:zebrafish|age:adult|collection date:2022 01 05|geo loc name:not collected|sex:female|tissue:gonad|replicate:replicate=biological replicate 5|BioSampleModel:Model organism or animal                 RNA seq of zebrafish zebra5 zebra5 normal RNA seq of zebrafish     RNA-Seq TRANSCRIPTOMIC Oligo-dT PAIRED ILLUMINA Illumina NovaSeq X Plus   SRP577302     L1EGC150008--ZF_H2.R1.raw.fastq.gz L1EGC150008--ZF_H2.R2.raw.fastq.gz fastq fastq 13690979438.0 45334369.0 L1EGC150008 ZF H2.R1.raw.fastq.gz 0:151 1:151 A:3525188290;C:3294780166;G:3472251867;T:3398718963;N:40152 151 151     3525188290 3294780166 3472251867 3398718963 40152 SRX28302713 SRS24645580 SRA2108762 South China Normal University|School of environment South China Normal University                       B B biological fallback assumption illumina novaseq_era unknown poly_a unknown bulk unknown unknown   China 2025-04-09 Adult Adult Gonad Reproductive System
35802 35802 SRR33037162 SRX28302712 SRS24645579 SRP577302 PRJNA1247234 Danio rerio Raw sequence reads PRJNA1247234 Whole Genome Sequencing normal RNA sequencing of gonads in Zebrafish Danio rerio         ZF High 1   breed:zebrafish|age:adult|collection date:2022 01 05|geo loc name:not collected|sex:female|tissue:gonad|replicate:replicate=biological replicate 4|BioSampleModel:Model organism or animal                 RNA seq of zebrafish zebra4 zebra4 normal RNA seq of zebrafish     RNA-Seq TRANSCRIPTOMIC Oligo-dT PAIRED ILLUMINA Illumina NovaSeq X Plus   SRP577302     L1EGC150007--ZF_H1.R1.raw.fastq.gz L1EGC150007--ZF_H1.R2.raw.fastq.gz fastq fastq 11255665632.0 37270416.0 L1EGC150007 ZF H1.R1.raw.fastq.gz 0:151 1:151 A:2843627240;C:2757680303;G:2890005499;T:2764318976;N:33614 151 151     2843627240 2757680303 2890005499 2764318976 33614 SRX28302712 SRS24645579 SRA2108762 South China Normal University|School of environment South China Normal University                       B B biological fallback assumption illumina novaseq_era unknown poly_a unknown bulk unknown unknown   China 2025-04-09 Adult Adult Gonad Reproductive System
35803 35803 SRR33037163 SRX28302711 SRS24645578 SRP577302 PRJNA1247234 Danio rerio Raw sequence reads PRJNA1247234 Whole Genome Sequencing normal RNA sequencing of gonads in Zebrafish Danio rerio         ZF Ctrl 3   breed:zebrafish|age:adult|collection date:2022 01 05|geo loc name:not collected|sex:female|tissue:gonad|replicate:replicate=biological replicate 3|BioSampleModel:Model organism or animal                 RNA seq of zebrafish zebra3 zebra3 normal RNA seq of zebrafish     RNA-Seq TRANSCRIPTOMIC Oligo-dT PAIRED ILLUMINA Illumina NovaSeq X Plus   SRP577302     L1EGC150006--ZF_C3.R1.raw.fastq.gz L1EGC150006--ZF_C3.R2.raw.fastq.gz fastq fastq 12437417302.0 41183501.0 L1EGC150006 ZF C3.R1.raw.fastq.gz 0:151 1:151 A:3190837806;C:3004347378;G:3147768574;T:3094427419;N:36125 151 151     3190837806 3004347378 3147768574 3094427419 36125 SRX28302711 SRS24645578 SRA2108762 South China Normal University|School of environment South China Normal University                       B B biological fallback assumption illumina novaseq_era unknown poly_a unknown bulk unknown unknown   China 2025-04-09 Adult Adult Gonad Reproductive System
35804 35804 SRR33037164 SRX28302710 SRS24645577 SRP577302 PRJNA1247234 Danio rerio Raw sequence reads PRJNA1247234 Whole Genome Sequencing normal RNA sequencing of gonads in Zebrafish Danio rerio         ZM High 3   breed:zebrafish|age:adult|collection date:2022 01 05|geo loc name:not collected|sex:male|tissue:gonad|replicate:replicate=biological replicate 12|BioSampleModel:Model organism or animal                 RNA seq of zebrafish zebra12 zebra12 normal RNA seq of zebrafish     RNA-Seq TRANSCRIPTOMIC Oligo-dT PAIRED ILLUMINA Illumina NovaSeq X Plus   SRP577302     L1EGC150015--ZM_H3.R1.raw.fastq.gz L1EGC150015--ZM_H3.R2.raw.fastq.gz fastq fastq 13403369738.0 44382019.0 L1EGC150015 ZM H3.R1.raw.fastq.gz 0:151 1:151 A:3502884741;C:3173528150;G:3354140507;T:3372777379;N:38961 151 151     3502884741 3173528150 3354140507 3372777379 38961 SRX28302710 SRS24645577 SRA2108762 South China Normal University|School of environment South China Normal University                       B B biological fallback assumption illumina novaseq_era unknown poly_a unknown bulk unknown unknown   China 2025-04-09 Adult Adult Gonad Reproductive System
35805 35805 SRR33037165 SRX28302709 SRS24645576 SRP577302 PRJNA1247234 Danio rerio Raw sequence reads PRJNA1247234 Whole Genome Sequencing normal RNA sequencing of gonads in Zebrafish Danio rerio         ZM High 2   breed:zebrafish|age:adult|collection date:2022 01 05|geo loc name:not collected|sex:male|tissue:gonad|replicate:replicate=biological replicate 11|BioSampleModel:Model organism or animal                 RNA seq of zebrafish zebra11 zebra11 normal RNA seq of zebrafish     RNA-Seq TRANSCRIPTOMIC Oligo-dT PAIRED ILLUMINA Illumina NovaSeq X Plus   SRP577302     L1EGC150014--ZM_H2.R1.raw.fastq.gz L1EGC150014--ZM_H2.R2.raw.fastq.gz fastq fastq 13821254084.0 45765742.0 L1EGC150014 ZM H2.R1.raw.fastq.gz 0:151 1:151 A:3608753052;C:3267421349;G:3457678530;T:3487360944;N:40209 151 151     3608753052 3267421349 3457678530 3487360944 40209 SRX28302709 SRS24645576 SRA2108762 South China Normal University|School of environment South China Normal University                       B B biological fallback assumption illumina novaseq_era unknown poly_a unknown bulk unknown unknown   China 2025-04-09 Adult Adult Gonad Reproductive System
35806 35806 SRR33037166 SRX28302708 SRS24645575 SRP577302 PRJNA1247234 Danio rerio Raw sequence reads PRJNA1247234 Whole Genome Sequencing normal RNA sequencing of gonads in Zebrafish Danio rerio         ZF Ctrl 2   breed:zebrafish|age:adult|collection date:2022 01 05|geo loc name:not collected|sex:female|tissue:gonad|replicate:replicate=biological replicate 2|BioSampleModel:Model organism or animal                 RNA seq of zebrafish zebra2 zebra2 normal RNA seq of zebrafish     RNA-Seq TRANSCRIPTOMIC Oligo-dT PAIRED ILLUMINA Illumina NovaSeq X Plus   SRP577302     L1EGC150005--ZF_C2.R1.raw.fastq.gz L1EGC150005--ZF_C2.R2.raw.fastq.gz fastq fastq 14593396812.0 48322506.0 L1EGC150005 ZF C2.R1.raw.fastq.gz 0:151 1:151 A:3717343233;C:3516949638;G:3746868538;T:3612192707;N:42696 151 151     3717343233 3516949638 3746868538 3612192707 42696 SRX28302708 SRS24645575 SRA2108762 South China Normal University|School of environment South China Normal University                       B B biological fallback assumption illumina novaseq_era unknown poly_a unknown bulk unknown unknown   China 2025-04-09 Adult Adult Gonad Reproductive System
35807 35807 SRR33037167 SRX28302707 SRS24645574 SRP577302 PRJNA1247234 Danio rerio Raw sequence reads PRJNA1247234 Whole Genome Sequencing normal RNA sequencing of gonads in Zebrafish Danio rerio         ZF Ctrl 1   breed:zebrafish|age:adult|collection date:2022 01 05|geo loc name:not collected|sex:female|tissue:gonad|replicate:replicate=biological replicate 1|BioSampleModel:Model organism or animal                 RNA seq of zebrafish zebra1 zebra1 normal RNA seq of zebrafish     RNA-Seq TRANSCRIPTOMIC Oligo-dT PAIRED ILLUMINA Illumina NovaSeq X Plus   SRP577302     L1EGC150004--ZF_C1.R1.raw.fastq.gz L1EGC150004--ZF_C1.R2.raw.fastq.gz fastq fastq 12184189396.0 40344998.0 L1EGC150004 ZF C1.R1.raw.fastq.gz 0:151 1:151 A:3187725727;C:2884827858;G:3037733456;T:3073866757;N:35598 151 151     3187725727 2884827858 3037733456 3073866757 35598 SRX28302707 SRS24645574 SRA2108762 South China Normal University|School of environment South China Normal University                       B B biological fallback assumption illumina novaseq_era unknown poly_a unknown bulk unknown unknown   China 2025-04-09 Adult Adult Gonad Reproductive System
50911 50911 SRR8377211 SRX5187246 SRS4193648 SRP174644 PRJNA512103 Danio rerio amh mutant RNA Seq PRJNA512103 Other For 21dpf and 35dpf juvenile zebrafish trunk tissue was isolated by removing the anterior of the fish from just posterior of the pectoral fin and removing the caudal peduncle posterior to the anus. For adults ovary and testis was dissected separately from other tissues. Total RNA was extracted using the Ribopure Kit Thermo Fisher and polyA mRNA was enriched using Dynabeads Thermo Fisher. We constructed indexed strand specific cDNA sequencing libraries using the NEXTflex™ qRNA seq kit BIOO Scientific. Libraries were normalized to 2.3nM multiplexed and evaluated by quantitative real time PCR using the Kapa Library Quantification Kit Kapa Biosystems. One lane of paired end 100 base pair bp sequencing was performed on an Illumina HiSeq 4000.   pubmed:31399485     Mut test8   strain:AB|age:adult|sex:male|tissue:testis|biomaterial provider:Zebrafish Facility at the University of Oregon|collected by:Yilin Yan|replicate:biological replicate 8 mutant testis|BioSampleModel:Model organism or animal                 Danio rerio amh mutant RNA seq Mut test8 Mut test8 Zebrafish were euthanized in Tricaine. We isolated gonad containing trunk of the animals by removing the anterior of the fish from just posterior of the pectoral fin and removing the caudal peduncle posterior to the anus. Trunks were individually homogenized in 200ul Trizol. Total RNA was extracted following Amores 2011. Total RNA was enriched for mRNA using Dynabeads Oligodt25 ThermoFisher. We constructed indexed strand specific cDNA sequencing libraries using the NEXTflex qRNA seq kit BIOO Scientific. Library concentrations were quantified using a Qubit fluorometer Life Technologies normalized to a concentration of 2.3nM and multiplexed. Prior to sequencing we further evaluated the quality of the multiplexed library by quantitative real time PCR using the Kapa Library Quantification Kit Kapa Biosystems. One lane of paired end 100 base pair bp sequencing was performed on an Illumina HiSeq 4000.     RNA-Seq TRANSCRIPTOMIC Oligo-dT PAIRED ILLUMINA Illumina HiSeq 4000   SRP174644     Mut_test8_R1.fq.gz Mut_test8_R2.fq.gz fastq fastq 921784580.0 4563290.0 Mut test8 R1.fq.gz 0:101 1:101 A:236359939;C:216070172;G:219949129;T:247094952;N:2310388 101 101     236359939 216070172 219949129 247094952 2310388 SRX5187246 SRS4193648 SRA827573 University of Oregon|Biology University of Oregon 2 0.78301 0.81043 0.13524 0.11867 0.65389 0.65723 0.42789 0.5722 101 101 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   United States 2019-09-06 Adult Adult Gonad Reproductive System
50912 50912 SRR8377212 SRX5187245 SRS4193647 SRP174644 PRJNA512103 Danio rerio amh mutant RNA Seq PRJNA512103 Other For 21dpf and 35dpf juvenile zebrafish trunk tissue was isolated by removing the anterior of the fish from just posterior of the pectoral fin and removing the caudal peduncle posterior to the anus. For adults ovary and testis was dissected separately from other tissues. Total RNA was extracted using the Ribopure Kit Thermo Fisher and polyA mRNA was enriched using Dynabeads Thermo Fisher. We constructed indexed strand specific cDNA sequencing libraries using the NEXTflex™ qRNA seq kit BIOO Scientific. Libraries were normalized to 2.3nM multiplexed and evaluated by quantitative real time PCR using the Kapa Library Quantification Kit Kapa Biosystems. One lane of paired end 100 base pair bp sequencing was performed on an Illumina HiSeq 4000.   pubmed:31399485     Mut test7   strain:AB|age:adult|sex:male|tissue:testis|biomaterial provider:Zebrafish Facility at the University of Oregon|collected by:Yilin Yan|replicate:biological replicate 7 mutant testis|BioSampleModel:Model organism or animal                 Danio rerio amh mutant RNA seq Mut test7 Mut test7 Zebrafish were euthanized in Tricaine. We isolated gonad containing trunk of the animals by removing the anterior of the fish from just posterior of the pectoral fin and removing the caudal peduncle posterior to the anus. Trunks were individually homogenized in 200ul Trizol. Total RNA was extracted following Amores 2011. Total RNA was enriched for mRNA using Dynabeads Oligodt25 ThermoFisher. We constructed indexed strand specific cDNA sequencing libraries using the NEXTflex qRNA seq kit BIOO Scientific. Library concentrations were quantified using a Qubit fluorometer Life Technologies normalized to a concentration of 2.3nM and multiplexed. Prior to sequencing we further evaluated the quality of the multiplexed library by quantitative real time PCR using the Kapa Library Quantification Kit Kapa Biosystems. One lane of paired end 100 base pair bp sequencing was performed on an Illumina HiSeq 4000.     RNA-Seq TRANSCRIPTOMIC Oligo-dT PAIRED ILLUMINA Illumina HiSeq 4000   SRP174644     Mut_test7_R1.fq.gz Mut_test7_R2.fq.gz fastq fastq 792655676.0 3924038.0 Mut test7 R1.fq.gz 0:101 1:101 A:207148130;C:182677007;G:184824169;T:216019509;N:1986861 101 101     207148130 182677007 184824169 216019509 1986861 SRX5187245 SRS4193647 SRA827573 University of Oregon|Biology University of Oregon 2 0.77877 0.79829 0.17788 0.15761 0.65494 0.66062 0.42697 0.58549 101 101 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   United States 2019-09-06 Adult Adult Gonad Reproductive System
50913 50913 SRR8377213 SRX5187244 SRS4193646 SRP174644 PRJNA512103 Danio rerio amh mutant RNA Seq PRJNA512103 Other For 21dpf and 35dpf juvenile zebrafish trunk tissue was isolated by removing the anterior of the fish from just posterior of the pectoral fin and removing the caudal peduncle posterior to the anus. For adults ovary and testis was dissected separately from other tissues. Total RNA was extracted using the Ribopure Kit Thermo Fisher and polyA mRNA was enriched using Dynabeads Thermo Fisher. We constructed indexed strand specific cDNA sequencing libraries using the NEXTflex™ qRNA seq kit BIOO Scientific. Libraries were normalized to 2.3nM multiplexed and evaluated by quantitative real time PCR using the Kapa Library Quantification Kit Kapa Biosystems. One lane of paired end 100 base pair bp sequencing was performed on an Illumina HiSeq 4000.   pubmed:31399485     Mut test6   strain:AB|age:adult|sex:male|tissue:testis|biomaterial provider:Zebrafish Facility at the University of Oregon|collected by:Yilin Yan|replicate:biological replicate 6 mutant testis|BioSampleModel:Model organism or animal                 Danio rerio amh mutant RNA seq Mut test6 Mut test6 Zebrafish were euthanized in Tricaine. We isolated gonad containing trunk of the animals by removing the anterior of the fish from just posterior of the pectoral fin and removing the caudal peduncle posterior to the anus. Trunks were individually homogenized in 200ul Trizol. Total RNA was extracted following Amores 2011. Total RNA was enriched for mRNA using Dynabeads Oligodt25 ThermoFisher. We constructed indexed strand specific cDNA sequencing libraries using the NEXTflex qRNA seq kit BIOO Scientific. Library concentrations were quantified using a Qubit fluorometer Life Technologies normalized to a concentration of 2.3nM and multiplexed. Prior to sequencing we further evaluated the quality of the multiplexed library by quantitative real time PCR using the Kapa Library Quantification Kit Kapa Biosystems. One lane of paired end 100 base pair bp sequencing was performed on an Illumina HiSeq 4000.     RNA-Seq TRANSCRIPTOMIC Oligo-dT PAIRED ILLUMINA Illumina HiSeq 4000   SRP174644     Mut_test6_R1.fq.gz Mut_test6_R2.fq.gz fastq fastq 995777584.0 4929592.0 Mut test6 R1.fq.gz 0:101 1:101 A:247361000;C:240902425;G:246039325;T:258988854;N:2485980 101 101     247361000 240902425 246039325 258988854 2485980 SRX5187244 SRS4193646 SRA827573 University of Oregon|Biology University of Oregon 2 0.80856 0.8345 0.11378 0.08191 0.69069 0.69445 0.41432 0.59146 101 101 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   United States 2019-09-06 Adult Adult Gonad Reproductive System
50914 50914 SRR8377214 SRX5187243 SRS4193645 SRP174644 PRJNA512103 Danio rerio amh mutant RNA Seq PRJNA512103 Other For 21dpf and 35dpf juvenile zebrafish trunk tissue was isolated by removing the anterior of the fish from just posterior of the pectoral fin and removing the caudal peduncle posterior to the anus. For adults ovary and testis was dissected separately from other tissues. Total RNA was extracted using the Ribopure Kit Thermo Fisher and polyA mRNA was enriched using Dynabeads Thermo Fisher. We constructed indexed strand specific cDNA sequencing libraries using the NEXTflex™ qRNA seq kit BIOO Scientific. Libraries were normalized to 2.3nM multiplexed and evaluated by quantitative real time PCR using the Kapa Library Quantification Kit Kapa Biosystems. One lane of paired end 100 base pair bp sequencing was performed on an Illumina HiSeq 4000.   pubmed:31399485     Mut test5   strain:AB|age:adult|sex:male|tissue:testis|biomaterial provider:Zebrafish Facility at the University of Oregon|collected by:Yilin Yan|replicate:biological replicate 5 mutant testis|BioSampleModel:Model organism or animal                 Danio rerio amh mutant RNA seq Mut test5 Mut test5 Zebrafish were euthanized in Tricaine. We isolated gonad containing trunk of the animals by removing the anterior of the fish from just posterior of the pectoral fin and removing the caudal peduncle posterior to the anus. Trunks were individually homogenized in 200ul Trizol. Total RNA was extracted following Amores 2011. Total RNA was enriched for mRNA using Dynabeads Oligodt25 ThermoFisher. We constructed indexed strand specific cDNA sequencing libraries using the NEXTflex qRNA seq kit BIOO Scientific. Library concentrations were quantified using a Qubit fluorometer Life Technologies normalized to a concentration of 2.3nM and multiplexed. Prior to sequencing we further evaluated the quality of the multiplexed library by quantitative real time PCR using the Kapa Library Quantification Kit Kapa Biosystems. One lane of paired end 100 base pair bp sequencing was performed on an Illumina HiSeq 4000.     RNA-Seq TRANSCRIPTOMIC Oligo-dT PAIRED ILLUMINA Illumina HiSeq 4000   SRP174644     Mut_test5_R1.fq.gz Mut_test5_R2.fq.gz fastq fastq 946685524.0 4686562.0 Mut test5 R1.fq.gz 0:101 1:101 A:244003750;C:220224385;G:224804319;T:255278005;N:2375065 101 101     244003750 220224385 224804319 255278005 2375065 SRX5187243 SRS4193645 SRA827573 University of Oregon|Biology University of Oregon 2 0.77946 0.80143 0.13073 0.11879 0.64707 0.65119 0.43756 0.57742 101 101 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   United States 2019-09-06 Adult Adult Gonad Reproductive System
50927 50927 SRR8377227 SRX5187230 SRS4193632 SRP174644 PRJNA512103 Danio rerio amh mutant RNA Seq PRJNA512103 Other For 21dpf and 35dpf juvenile zebrafish trunk tissue was isolated by removing the anterior of the fish from just posterior of the pectoral fin and removing the caudal peduncle posterior to the anus. For adults ovary and testis was dissected separately from other tissues. Total RNA was extracted using the Ribopure Kit Thermo Fisher and polyA mRNA was enriched using Dynabeads Thermo Fisher. We constructed indexed strand specific cDNA sequencing libraries using the NEXTflex™ qRNA seq kit BIOO Scientific. Libraries were normalized to 2.3nM multiplexed and evaluated by quantitative real time PCR using the Kapa Library Quantification Kit Kapa Biosystems. One lane of paired end 100 base pair bp sequencing was performed on an Illumina HiSeq 4000.   pubmed:31399485     Mut ovar3   strain:AB|age:adult|sex:female|tissue:ovary|biomaterial provider:Zebrafish Facility at the University of Oregon|collected by:Yilin Yan|replicate:biological replicate 3 mutant ovary|BioSampleModel:Model organism or animal                 Danio rerio amh mutant RNA seq Mut ovar3 Mut ovar3 Zebrafish were euthanized in Tricaine. We isolated gonad containing trunk of the animals by removing the anterior of the fish from just posterior of the pectoral fin and removing the caudal peduncle posterior to the anus. Trunks were individually homogenized in 200ul Trizol. Total RNA was extracted following {Amores 2011 #69}. Total RNA was enriched for mRNA using Dynabeads Oligodt25 ThermoFisher. We constructed indexed strand specific cDNA sequencing libraries using the NEXTflex qRNA seq kit BIOO Scientific. Library concentrations were quantified using a Qubit fluorometer Life Technologies normalized to a concentration of 2.3nM and multiplexed. Prior to sequencing we further evaluated the quality of the multiplexed library by quantitative real time PCR using the Kapa Library Quantification Kit Kapa Biosystems. One lane of paired end 100 base pair bp sequencing was performed on an Illumina HiSeq 4000.     RNA-Seq TRANSCRIPTOMIC Oligo-dT PAIRED ILLUMINA Illumina HiSeq 4000   SRP174644     Mut_ovar3_R1.fq.gz Mut_ovar3_R2.fq.gz fastq fastq 2540566322.0 12577061.0 Mut ovar3 R1.fq.gz 0:101 1:101 A:642328988;C:595153938;G:613795728;T:682917257;N:6370411 101 101     642328988 595153938 613795728 682917257 6370411 SRX5187230 SRS4193632 SRA827573 University of Oregon|Biology University of Oregon 2 0.8031 0.82038 0.02205 0.01952 0.76027 0.76445 0.44826 0.52398 101 101 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   United States 2019-09-06 Adult Adult Gonad Reproductive System
50928 50928 SRR8377228 SRX5187229 SRS4193631 SRP174644 PRJNA512103 Danio rerio amh mutant RNA Seq PRJNA512103 Other For 21dpf and 35dpf juvenile zebrafish trunk tissue was isolated by removing the anterior of the fish from just posterior of the pectoral fin and removing the caudal peduncle posterior to the anus. For adults ovary and testis was dissected separately from other tissues. Total RNA was extracted using the Ribopure Kit Thermo Fisher and polyA mRNA was enriched using Dynabeads Thermo Fisher. We constructed indexed strand specific cDNA sequencing libraries using the NEXTflex™ qRNA seq kit BIOO Scientific. Libraries were normalized to 2.3nM multiplexed and evaluated by quantitative real time PCR using the Kapa Library Quantification Kit Kapa Biosystems. One lane of paired end 100 base pair bp sequencing was performed on an Illumina HiSeq 4000.   pubmed:31399485     Mut ovar4   strain:AB|age:adult|sex:female|tissue:ovary|biomaterial provider:Zebrafish Facility at the University of Oregon|collected by:Yilin Yan|replicate:biological replicate 4 mutant ovary|BioSampleModel:Model organism or animal                 Danio rerio amh mutant RNA seq Mut ovar4 Mut ovar4 Zebrafish were euthanized in Tricaine. We isolated gonad containing trunk of the animals by removing the anterior of the fish from just posterior of the pectoral fin and removing the caudal peduncle posterior to the anus. Trunks were individually homogenized in 200ul Trizol. Total RNA was extracted following Amores 2011. Total RNA was enriched for mRNA using Dynabeads Oligodt25 ThermoFisher. We constructed indexed strand specific cDNA sequencing libraries using the NEXTflex qRNA seq kit BIOO Scientific. Library concentrations were quantified using a Qubit fluorometer Life Technologies normalized to a concentration of 2.3nM and multiplexed. Prior to sequencing we further evaluated the quality of the multiplexed library by quantitative real time PCR using the Kapa Library Quantification Kit Kapa Biosystems. One lane of paired end 100 base pair bp sequencing was performed on an Illumina HiSeq 4000.     RNA-Seq TRANSCRIPTOMIC Oligo-dT PAIRED ILLUMINA Illumina HiSeq 4000   SRP174644     Mut_ovar4_R1.fq.gz Mut_ovar4_R2.fq.gz fastq fastq 954221336.0 4723868.0 Mut ovar4 R1.fq.gz 0:101 1:101 A:240656530;C:224543348;G:233050024;T:253576315;N:2395119 101 101     240656530 224543348 233050024 253576315 2395119 SRX5187229 SRS4193631 SRA827573 University of Oregon|Biology University of Oregon 2 0.79917 0.81767 0.02406 0.02019 0.76079 0.76587 0.4452 0.52439 101 101 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   United States 2019-09-06 Adult Adult Gonad Reproductive System
50929 50929 SRR8377229 SRX5187228 SRS4193630 SRP174644 PRJNA512103 Danio rerio amh mutant RNA Seq PRJNA512103 Other For 21dpf and 35dpf juvenile zebrafish trunk tissue was isolated by removing the anterior of the fish from just posterior of the pectoral fin and removing the caudal peduncle posterior to the anus. For adults ovary and testis was dissected separately from other tissues. Total RNA was extracted using the Ribopure Kit Thermo Fisher and polyA mRNA was enriched using Dynabeads Thermo Fisher. We constructed indexed strand specific cDNA sequencing libraries using the NEXTflex™ qRNA seq kit BIOO Scientific. Libraries were normalized to 2.3nM multiplexed and evaluated by quantitative real time PCR using the Kapa Library Quantification Kit Kapa Biosystems. One lane of paired end 100 base pair bp sequencing was performed on an Illumina HiSeq 4000.   pubmed:31399485     Mut ovar1   strain:AB|age:adult|sex:female|tissue:ovary|biomaterial provider:Zebrafish Facility at the University of Oregon|collected by:Yilin Yan|replicate:biological replicate 1 mutant ovary|BioSampleModel:Model organism or animal                 Danio rerio amh mutant RNA seq Mut ovar1 Mut ovar1 Zebrafish were euthanized in Tricaine. We isolated gonad containing trunk of the animals by removing the anterior of the fish from just posterior of the pectoral fin and removing the caudal peduncle posterior to the anus. Trunks were individually homogenized in 200ul Trizol. Total RNA was extracted following {Amores 2011 #69}. Total RNA was enriched for mRNA using Dynabeads Oligodt25 ThermoFisher. We constructed indexed strand specific cDNA sequencing libraries using the NEXTflex qRNA seq kit BIOO Scientific. Library concentrations were quantified using a Qubit fluorometer Life Technologies normalized to a concentration of 2.3nM and multiplexed. Prior to sequencing we further evaluated the quality of the multiplexed library by quantitative real time PCR using the Kapa Library Quantification Kit Kapa Biosystems. One lane of paired end 100 base pair bp sequencing was performed on an Illumina HiSeq 4000.     RNA-Seq TRANSCRIPTOMIC Oligo-dT PAIRED ILLUMINA Illumina HiSeq 4000   SRP174644     Mut_ovar1_R2.fq.gz Mut_ovar1_R1.fq.gz fastq fastq 1261230632.0 6243716.0 Mut ovar1 R1.fq.gz 0:101 1:101 A:319192722;C:298433558;G:306822515;T:333627695;N:3154142 101 101     319192722 298433558 306822515 333627695 3154142 SRX5187228 SRS4193630 SRA827573 University of Oregon|Biology University of Oregon 2 0.78419 0.8175 0.03057 0.02643 0.76518 0.7681 0.43612 0.52079 101 101 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   United States 2018-12-28 Adult Adult Gonad Reproductive System
50930 50930 SRR8377230 SRX5187227 SRS4193629 SRP174644 PRJNA512103 Danio rerio amh mutant RNA Seq PRJNA512103 Other For 21dpf and 35dpf juvenile zebrafish trunk tissue was isolated by removing the anterior of the fish from just posterior of the pectoral fin and removing the caudal peduncle posterior to the anus. For adults ovary and testis was dissected separately from other tissues. Total RNA was extracted using the Ribopure Kit Thermo Fisher and polyA mRNA was enriched using Dynabeads Thermo Fisher. We constructed indexed strand specific cDNA sequencing libraries using the NEXTflex™ qRNA seq kit BIOO Scientific. Libraries were normalized to 2.3nM multiplexed and evaluated by quantitative real time PCR using the Kapa Library Quantification Kit Kapa Biosystems. One lane of paired end 100 base pair bp sequencing was performed on an Illumina HiSeq 4000.   pubmed:31399485     Mut ovar2   strain:AB|age:adult|sex:female|tissue:ovary|biomaterial provider:Zebrafish Facility at the University of Oregon|collected by:Yilin Yan|replicate:biological replicate 2 mutant ovary|BioSampleModel:Model organism or animal                 Danio rerio amh mutant RNA seq Mut ovar2 Mut ovar2 Zebrafish were euthanized in Tricaine. We isolated gonad containing trunk of the animals by removing the anterior of the fish from just posterior of the pectoral fin and removing the caudal peduncle posterior to the anus. Trunks were individually homogenized in 200ul Trizol. Total RNA was extracted following {Amores 2011 #69}. Total RNA was enriched for mRNA using Dynabeads Oligodt25 ThermoFisher. We constructed indexed strand specific cDNA sequencing libraries using the NEXTflex qRNA seq kit BIOO Scientific. Library concentrations were quantified using a Qubit fluorometer Life Technologies normalized to a concentration of 2.3nM and multiplexed. Prior to sequencing we further evaluated the quality of the multiplexed library by quantitative real time PCR using the Kapa Library Quantification Kit Kapa Biosystems. One lane of paired end 100 base pair bp sequencing was performed on an Illumina HiSeq 4000.     RNA-Seq TRANSCRIPTOMIC Oligo-dT PAIRED ILLUMINA Illumina HiSeq 4000   SRP174644     Mut_ovar2_R1.fq.gz Mut_ovar2_R2.fq.gz fastq fastq 732858626.0 3628013.0 Mut ovar2 R1.fq.gz 0:101 1:101 A:188242301;C:168675201;G:175286662;T:198821957;N:1832505 101 101     188242301 168675201 175286662 198821957 1832505 SRX5187227 SRS4193629 SRA827573 University of Oregon|Biology University of Oregon 2 0.80673 0.81536 0.02204 0.01994 0.7627 0.76566 0.43189 0.50864 101 101 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   United States 2019-09-06 Adult Adult Gonad Reproductive System
50946 50946 SRR8377246 SRX5187211 SRS4193613 SRP174644 PRJNA512103 Danio rerio amh mutant RNA Seq PRJNA512103 Other For 21dpf and 35dpf juvenile zebrafish trunk tissue was isolated by removing the anterior of the fish from just posterior of the pectoral fin and removing the caudal peduncle posterior to the anus. For adults ovary and testis was dissected separately from other tissues. Total RNA was extracted using the Ribopure Kit Thermo Fisher and polyA mRNA was enriched using Dynabeads Thermo Fisher. We constructed indexed strand specific cDNA sequencing libraries using the NEXTflex™ qRNA seq kit BIOO Scientific. Libraries were normalized to 2.3nM multiplexed and evaluated by quantitative real time PCR using the Kapa Library Quantification Kit Kapa Biosystems. One lane of paired end 100 base pair bp sequencing was performed on an Illumina HiSeq 4000.   pubmed:31399485     WT ovar1   strain:AB|age:adult|sex:female|tissue:ovary|biomaterial provider:Zebrafish Facility at the University of Oregon|collected by:Yilin Yan|replicate:biological replicate 1 wild type ovary|BioSampleModel:Model organism or animal                 Danio rerio amh mutant RNA seq WT ovar1 WT ovar1 Zebrafish were euthanized in Tricaine. We isolated gonad containing trunk of the animals by removing the anterior of the fish from just posterior of the pectoral fin and removing the caudal peduncle posterior to the anus. Trunks were individually homogenized in 200ul Trizol. Total RNA was extracted following Amores 2011. Total RNA was enriched for mRNA using Dynabeads Oligodt25 ThermoFisher. We constructed indexed strand specific cDNA sequencing libraries using the NEXTflex qRNA seq kit BIOO Scientific. Library concentrations were quantified using a Qubit fluorometer Life Technologies normalized to a concentration of 2.3nM and multiplexed. Prior to sequencing we further evaluated the quality of the multiplexed library by quantitative real time PCR using the Kapa Library Quantification Kit Kapa Biosystems. One lane of paired end 100 base pair bp sequencing was performed on an Illumina HiSeq 4000.     RNA-Seq TRANSCRIPTOMIC Oligo-dT PAIRED ILLUMINA Illumina HiSeq 4000   SRP174644     WT_ovar1_R1.fq.gz WT_ovar1_R2.fq.gz fastq fastq 665032278.0 3292239.0 WT ovar1 R1.fq.gz 0:101 1:101 A:167152394;C:159953694;G:162556883;T:173695536;N:1673771 101 101     167152394 159953694 162556883 173695536 1673771 SRX5187211 SRS4193613 SRA827573 University of Oregon|Biology University of Oregon 2 0.77305 0.7925 0.03303 0.03049 0.76221 0.764 0.44152 0.53096 101 101 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   United States 2019-09-06 Adult Adult Gonad Reproductive System
50947 50947 SRR8377247 SRX5187210 SRS4193612 SRP174644 PRJNA512103 Danio rerio amh mutant RNA Seq PRJNA512103 Other For 21dpf and 35dpf juvenile zebrafish trunk tissue was isolated by removing the anterior of the fish from just posterior of the pectoral fin and removing the caudal peduncle posterior to the anus. For adults ovary and testis was dissected separately from other tissues. Total RNA was extracted using the Ribopure Kit Thermo Fisher and polyA mRNA was enriched using Dynabeads Thermo Fisher. We constructed indexed strand specific cDNA sequencing libraries using the NEXTflex™ qRNA seq kit BIOO Scientific. Libraries were normalized to 2.3nM multiplexed and evaluated by quantitative real time PCR using the Kapa Library Quantification Kit Kapa Biosystems. One lane of paired end 100 base pair bp sequencing was performed on an Illumina HiSeq 4000.   pubmed:31399485     WT ovar3   strain:AB|age:adult|sex:female|tissue:ovary|biomaterial provider:Zebrafish Facility at the University of Oregon|collected by:Yilin Yan|replicate:biological replicate 3 wild type ovary|BioSampleModel:Model organism or animal                 Danio rerio amh mutant RNA seq WT ovar3 WT ovar3 Zebrafish were euthanized in Tricaine. We isolated gonad containing trunk of the animals by removing the anterior of the fish from just posterior of the pectoral fin and removing the caudal peduncle posterior to the anus. Trunks were individually homogenized in 200ul Trizol. Total RNA was extracted following Amores 2011. Total RNA was enriched for mRNA using Dynabeads Oligodt25 ThermoFisher. We constructed indexed strand specific cDNA sequencing libraries using the NEXTflex qRNA seq kit BIOO Scientific. Library concentrations were quantified using a Qubit fluorometer Life Technologies normalized to a concentration of 2.3nM and multiplexed. Prior to sequencing we further evaluated the quality of the multiplexed library by quantitative real time PCR using the Kapa Library Quantification Kit Kapa Biosystems. One lane of paired end 100 base pair bp sequencing was performed on an Illumina HiSeq 4000.     RNA-Seq TRANSCRIPTOMIC Oligo-dT PAIRED ILLUMINA Illumina HiSeq 4000   SRP174644     WT_ovar3_R1.fq.gz WT_ovar3_R2.fq.gz fastq fastq 924941234.0 4578917.0 WT ovar3 R1.fq.gz 0:101 1:101 A:231521603;C:222222435;G:226308259;T:242575970;N:2312967 101 101     231521603 222222435 226308259 242575970 2312967 SRX5187210 SRS4193612 SRA827573 University of Oregon|Biology University of Oregon 2 0.78319 0.81024 0.03697 0.03451 0.75457 0.75503 0.44498 0.54403 101 101 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   United States 2019-09-06 Adult Adult Gonad Reproductive System
50948 50948 SRR8377248 SRX5187209 SRS4193611 SRP174644 PRJNA512103 Danio rerio amh mutant RNA Seq PRJNA512103 Other For 21dpf and 35dpf juvenile zebrafish trunk tissue was isolated by removing the anterior of the fish from just posterior of the pectoral fin and removing the caudal peduncle posterior to the anus. For adults ovary and testis was dissected separately from other tissues. Total RNA was extracted using the Ribopure Kit Thermo Fisher and polyA mRNA was enriched using Dynabeads Thermo Fisher. We constructed indexed strand specific cDNA sequencing libraries using the NEXTflex™ qRNA seq kit BIOO Scientific. Libraries were normalized to 2.3nM multiplexed and evaluated by quantitative real time PCR using the Kapa Library Quantification Kit Kapa Biosystems. One lane of paired end 100 base pair bp sequencing was performed on an Illumina HiSeq 4000.   pubmed:31399485     WT ovar2   strain:AB|age:adult|sex:female|tissue:ovary|biomaterial provider:Zebrafish Facility at the University of Oregon|collected by:Yilin Yan|replicate:biological replicate 2 wild type ovary|BioSampleModel:Model organism or animal                 Danio rerio amh mutant RNA seq WT ovar2 WT ovar2 Zebrafish were euthanized in Tricaine. We isolated gonad containing trunk of the animals by removing the anterior of the fish from just posterior of the pectoral fin and removing the caudal peduncle posterior to the anus. Trunks were individually homogenized in 200ul Trizol. Total RNA was extracted following Amores 2011. Total RNA was enriched for mRNA using Dynabeads Oligodt25 ThermoFisher. We constructed indexed strand specific cDNA sequencing libraries using the NEXTflex qRNA seq kit BIOO Scientific. Library concentrations were quantified using a Qubit fluorometer Life Technologies normalized to a concentration of 2.3nM and multiplexed. Prior to sequencing we further evaluated the quality of the multiplexed library by quantitative real time PCR using the Kapa Library Quantification Kit Kapa Biosystems. One lane of paired end 100 base pair bp sequencing was performed on an Illumina HiSeq 4000.     RNA-Seq TRANSCRIPTOMIC Oligo-dT PAIRED ILLUMINA Illumina HiSeq 4000   SRP174644     WT_ovar2_R1.fq.gz WT_ovar2_R2.fq.gz fastq fastq 1711791834.0 8474217.0 WT ovar2 R1.fq.gz 0:101 1:101 A:422009453;C:414454112;G:427618045;T:443430653;N:4279571 101 101     422009453 414454112 427618045 443430653 4279571 SRX5187209 SRS4193611 SRA827573 University of Oregon|Biology University of Oregon 2 0.80961 0.83405 0.04196 0.03648 0.75043 0.75509 0.46063 0.53569 101 101 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   United States 2019-09-06 Adult Adult Gonad Reproductive System
50949 50949 SRR8377249 SRX5187208 SRS4193610 SRP174644 PRJNA512103 Danio rerio amh mutant RNA Seq PRJNA512103 Other For 21dpf and 35dpf juvenile zebrafish trunk tissue was isolated by removing the anterior of the fish from just posterior of the pectoral fin and removing the caudal peduncle posterior to the anus. For adults ovary and testis was dissected separately from other tissues. Total RNA was extracted using the Ribopure Kit Thermo Fisher and polyA mRNA was enriched using Dynabeads Thermo Fisher. We constructed indexed strand specific cDNA sequencing libraries using the NEXTflex™ qRNA seq kit BIOO Scientific. Libraries were normalized to 2.3nM multiplexed and evaluated by quantitative real time PCR using the Kapa Library Quantification Kit Kapa Biosystems. One lane of paired end 100 base pair bp sequencing was performed on an Illumina HiSeq 4000.   pubmed:31399485     WT test1   strain:AB|age:adult|sex:male|tissue:testis|biomaterial provider:Zebrafish Facility at the University of Oregon|collected by:Yilin Yan|replicate:biological replicate 1 wild type testis|BioSampleModel:Model organism or animal                 Danio rerio amh mutant RNA seq WT test1 WT test1 Zebrafish were euthanized in Tricaine. We isolated gonad containing trunk of the animals by removing the anterior of the fish from just posterior of the pectoral fin and removing the caudal peduncle posterior to the anus. Trunks were individually homogenized in 200ul Trizol. Total RNA was extracted following Amores 2011. Total RNA was enriched for mRNA using Dynabeads Oligodt25 ThermoFisher. We constructed indexed strand specific cDNA sequencing libraries using the NEXTflex qRNA seq kit BIOO Scientific. Library concentrations were quantified using a Qubit fluorometer Life Technologies normalized to a concentration of 2.3nM and multiplexed. Prior to sequencing we further evaluated the quality of the multiplexed library by quantitative real time PCR using the Kapa Library Quantification Kit Kapa Biosystems. One lane of paired end 100 base pair bp sequencing was performed on an Illumina HiSeq 4000.     RNA-Seq TRANSCRIPTOMIC Oligo-dT PAIRED ILLUMINA Illumina HiSeq 4000   SRP174644     WT_test1_R2.fq.gz WT_test1_R1.fq.gz fastq fastq 2303992608.0 11405904.0 WT test1 R1.fq.gz 0:101 1:101 A:567633881;C:563967121;G:572235529;T:594400255;N:5755822 101 101     567633881 563967121 572235529 594400255 5755822 SRX5187208 SRS4193610 SRA827573 University of Oregon|Biology University of Oregon 2 0.80661 0.83085 0.17247 0.1314 0.69023 0.69094 0.39549 0.64674 101 101 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   United States 2019-09-06 Adult Adult Gonad Reproductive System
50950 50950 SRR8377250 SRX5187207 SRS4193609 SRP174644 PRJNA512103 Danio rerio amh mutant RNA Seq PRJNA512103 Other For 21dpf and 35dpf juvenile zebrafish trunk tissue was isolated by removing the anterior of the fish from just posterior of the pectoral fin and removing the caudal peduncle posterior to the anus. For adults ovary and testis was dissected separately from other tissues. Total RNA was extracted using the Ribopure Kit Thermo Fisher and polyA mRNA was enriched using Dynabeads Thermo Fisher. We constructed indexed strand specific cDNA sequencing libraries using the NEXTflex™ qRNA seq kit BIOO Scientific. Libraries were normalized to 2.3nM multiplexed and evaluated by quantitative real time PCR using the Kapa Library Quantification Kit Kapa Biosystems. One lane of paired end 100 base pair bp sequencing was performed on an Illumina HiSeq 4000.   pubmed:31399485     WT ovar4   strain:AB|age:adult|sex:female|tissue:ovary|biomaterial provider:Zebrafish Facility at the University of Oregon|collected by:Yilin Yan|replicate:biological replicate 4 wild type ovary|BioSampleModel:Model organism or animal                 Danio rerio amh mutant RNA seq WT ovar4 WT ovar4 Zebrafish were euthanized in Tricaine. We isolated gonad containing trunk of the animals by removing the anterior of the fish from just posterior of the pectoral fin and removing the caudal peduncle posterior to the anus. Trunks were individually homogenized in 200ul Trizol. Total RNA was extracted following Amores 2011. Total RNA was enriched for mRNA using Dynabeads Oligodt25 ThermoFisher. We constructed indexed strand specific cDNA sequencing libraries using the NEXTflex qRNA seq kit BIOO Scientific. Library concentrations were quantified using a Qubit fluorometer Life Technologies normalized to a concentration of 2.3nM and multiplexed. Prior to sequencing we further evaluated the quality of the multiplexed library by quantitative real time PCR using the Kapa Library Quantification Kit Kapa Biosystems. One lane of paired end 100 base pair bp sequencing was performed on an Illumina HiSeq 4000.     RNA-Seq TRANSCRIPTOMIC Oligo-dT PAIRED ILLUMINA Illumina HiSeq 4000   SRP174644     WT_ovar4_R1.fq.gz WT_ovar4_R2.fq.gz fastq fastq 3313457712.0 16403256.0 WT ovar4 R1.fq.gz 0:101 1:101 A:819658009;C:805343322;G:820126137;T:860008778;N:8321466 101 101     819658009 805343322 820126137 860008778 8321466 SRX5187207 SRS4193609 SRA827573 University of Oregon|Biology University of Oregon 2 0.7758 0.80453 0.03627 0.03327 0.75877 0.75923 0.4509 0.53523 101 101 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   United States 2019-09-06 Adult Adult Gonad Reproductive System
50951 50951 SRR8377251 SRX5187206 SRS4193608 SRP174644 PRJNA512103 Danio rerio amh mutant RNA Seq PRJNA512103 Other For 21dpf and 35dpf juvenile zebrafish trunk tissue was isolated by removing the anterior of the fish from just posterior of the pectoral fin and removing the caudal peduncle posterior to the anus. For adults ovary and testis was dissected separately from other tissues. Total RNA was extracted using the Ribopure Kit Thermo Fisher and polyA mRNA was enriched using Dynabeads Thermo Fisher. We constructed indexed strand specific cDNA sequencing libraries using the NEXTflex™ qRNA seq kit BIOO Scientific. Libraries were normalized to 2.3nM multiplexed and evaluated by quantitative real time PCR using the Kapa Library Quantification Kit Kapa Biosystems. One lane of paired end 100 base pair bp sequencing was performed on an Illumina HiSeq 4000.   pubmed:31399485     WT test4   strain:AB|age:adult|sex:male|tissue:testis|biomaterial provider:Zebrafish Facility at the University of Oregon|collected by:Yilin Yan|replicate:biological replicate 4 wild type testis|BioSampleModel:Model organism or animal                 Danio rerio amh mutant RNA seq WT test4 WT test4 Zebrafish were euthanized in Tricaine. We isolated gonad containing trunk of the animals by removing the anterior of the fish from just posterior of the pectoral fin and removing the caudal peduncle posterior to the anus. Trunks were individually homogenized in 200ul Trizol. Total RNA was extracted following Amores 2011. Total RNA was enriched for mRNA using Dynabeads Oligodt25 ThermoFisher. We constructed indexed strand specific cDNA sequencing libraries using the NEXTflex qRNA seq kit BIOO Scientific. Library concentrations were quantified using a Qubit fluorometer Life Technologies normalized to a concentration of 2.3nM and multiplexed. Prior to sequencing we further evaluated the quality of the multiplexed library by quantitative real time PCR using the Kapa Library Quantification Kit Kapa Biosystems. One lane of paired end 100 base pair bp sequencing was performed on an Illumina HiSeq 4000.     RNA-Seq TRANSCRIPTOMIC Oligo-dT PAIRED ILLUMINA Illumina HiSeq 4000   SRP174644     WT_test4_R1.fq.gz WT_test4_R2.fq.gz fastq fastq 3904257818.0 19328009.0 WT test4 R1.fq.gz 0:101 1:101 A:975797484;C:942810562;G:953761060;T:1022122312;N:9766400 101 101     975797484 942810562 953761060 1022122312 9766400 SRX5187206 SRS4193608 SRA827573 University of Oregon|Biology University of Oregon 2 0.80828 0.82737 0.14204 0.11127 0.67629 0.67795 0.41259 0.58947 101 101 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   United States 2019-09-06 Adult Adult Gonad Reproductive System
50952 50952 SRR8377252 SRX5187205 SRS4193607 SRP174644 PRJNA512103 Danio rerio amh mutant RNA Seq PRJNA512103 Other For 21dpf and 35dpf juvenile zebrafish trunk tissue was isolated by removing the anterior of the fish from just posterior of the pectoral fin and removing the caudal peduncle posterior to the anus. For adults ovary and testis was dissected separately from other tissues. Total RNA was extracted using the Ribopure Kit Thermo Fisher and polyA mRNA was enriched using Dynabeads Thermo Fisher. We constructed indexed strand specific cDNA sequencing libraries using the NEXTflex™ qRNA seq kit BIOO Scientific. Libraries were normalized to 2.3nM multiplexed and evaluated by quantitative real time PCR using the Kapa Library Quantification Kit Kapa Biosystems. One lane of paired end 100 base pair bp sequencing was performed on an Illumina HiSeq 4000.   pubmed:31399485     WT test2   strain:AB|age:adult|sex:male|tissue:testis|biomaterial provider:Zebrafish Facility at the University of Oregon|collected by:Yilin Yan|replicate:biological replicate 2 wild type testis|BioSampleModel:Model organism or animal                 Danio rerio amh mutant RNA seq WT test2 WT test2 Zebrafish were euthanized in Tricaine. We isolated gonad containing trunk of the animals by removing the anterior of the fish from just posterior of the pectoral fin and removing the caudal peduncle posterior to the anus. Trunks were individually homogenized in 200ul Trizol. Total RNA was extracted following Amores 2011. Total RNA was enriched for mRNA using Dynabeads Oligodt25 ThermoFisher. We constructed indexed strand specific cDNA sequencing libraries using the NEXTflex qRNA seq kit BIOO Scientific. Library concentrations were quantified using a Qubit fluorometer Life Technologies normalized to a concentration of 2.3nM and multiplexed. Prior to sequencing we further evaluated the quality of the multiplexed library by quantitative real time PCR using the Kapa Library Quantification Kit Kapa Biosystems. One lane of paired end 100 base pair bp sequencing was performed on an Illumina HiSeq 4000.     RNA-Seq TRANSCRIPTOMIC Oligo-dT PAIRED ILLUMINA Illumina HiSeq 4000   SRP174644     WT_test2_R1.fq.gz WT_test2_R2.fq.gz fastq fastq 1018330278.0 5041239.0 WT test2 R1.fq.gz 0:101 1:101 A:248547473;C:252682009;G:254659891;T:259887061;N:2553844 101 101     248547473 252682009 254659891 259887061 2553844 SRX5187205 SRS4193607 SRA827573 University of Oregon|Biology University of Oregon 2 0.81265 0.83613 0.19103 0.13656 0.69493 0.69495 0.37369 0.66738 101 101 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   United States 2019-09-06 Adult Adult Gonad Reproductive System
69479 69479 SRR18731940 SRX14832179 SRS12587950 SRP369810 PRJNA825753 Zebrafish ovarian transcriptome sequencing PRJNA825753 Other 1 The transcriptome sequencing of nine samples was completed and a total of 63.66 Gb Clean Data was obtained and the Clean Data of each sample reached 6.14 Gb and above and the percentage of Q30 bases was 91.64 % and above.2 The Clean Reads of each sample were compared with the designated reference genome for sequence alignment and the alignment efficiency was 92.04% or more.3 Functional annotation was performed for all genes including comparison with NR [1] Swiss Prot [2] KEGG [3] COG [4] KOG [5] GO [6] and Pfam databases and annotation results for a total of 26929 genes were obtained.4 Based on the comparison results the libraries were evaluated.5 Based on the comparison results gene expression analysis was performed. Based on the expression of genes in different samples differentially expressed genes were identified and functional annotation and enrichment analysis were performed.         Zebrafish Ovaries   strain:not applicable|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|age:6 Months|dev stage:adult fish|sex:female|tissue:Ovaries|BioSampleModel:Model organism or animal                 Adult zebrafish ovary 20220412 9 20220412 9 materials and methods     RNA-Seq TRANSCRIPTOMIC Oligo-dT PAIRED ILLUMINA Illumina HiSeq 2500   SRP369810     GPR19-11-Rescue-3_BKDL202619368-1a-N711-AK415_1.fq.gz GPR19-11-Rescue-3_BKDL202619368-1a-N711-AK415_2.fq.gz fastq fastq 7830567600.0 26101892.0 GPR19 11 Rescue 3 BKDL202619368 1a N711 AK415 1.fq.gz 0:150 1:150 A:2091186346;C:1821842935;G:1856665826;T:2060841839;N:30654 150 150     2091186346 1821842935 1856665826 2060841839 30654 SRX14832179 SRS12587950 SRA1402797 zhejiang ocean university|school of fishery zhejiang ocean university 2 0.94642 0.94446 0.03159 0.031 0.73977 0.74101 0.49671 0.49184 150 150 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   China 2022-04-12 Adult Adult Gonad Reproductive System
69480 69480 SRR18731941 SRX14832178 SRS12587950 SRP369810 PRJNA825753 Zebrafish ovarian transcriptome sequencing PRJNA825753 Other 1 The transcriptome sequencing of nine samples was completed and a total of 63.66 Gb Clean Data was obtained and the Clean Data of each sample reached 6.14 Gb and above and the percentage of Q30 bases was 91.64 % and above.2 The Clean Reads of each sample were compared with the designated reference genome for sequence alignment and the alignment efficiency was 92.04% or more.3 Functional annotation was performed for all genes including comparison with NR [1] Swiss Prot [2] KEGG [3] COG [4] KOG [5] GO [6] and Pfam databases and annotation results for a total of 26929 genes were obtained.4 Based on the comparison results the libraries were evaluated.5 Based on the comparison results gene expression analysis was performed. Based on the expression of genes in different samples differentially expressed genes were identified and functional annotation and enrichment analysis were performed.         Zebrafish Ovaries   strain:not applicable|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|age:6 Months|dev stage:adult fish|sex:female|tissue:Ovaries|BioSampleModel:Model organism or animal                 Adult zebrafish ovary 20220412 8 20220412 8 materials and methods     RNA-Seq TRANSCRIPTOMIC Oligo-dT PAIRED ILLUMINA Illumina HiSeq 2500   SRP369810     GPR19-11-Rescue-2_BKDL202619368-1a-N710-AK416_1.fq.gz GPR19-11-Rescue-2_BKDL202619368-1a-N710-AK416_2.fq.gz fastq fastq 6295515300.0 20985051.0 GPR19 11 Rescue 2 BKDL202619368 1a N710 AK416 1.fq.gz 0:150 1:150 A:1677101982;C:1466375952;G:1498052845;T:1653959638;N:24883 150 150     1677101982 1466375952 1498052845 1653959638 24883 SRX14832178 SRS12587950 SRA1402797 zhejiang ocean university|school of fishery zhejiang ocean university 2 0.94529 0.94401 0.0307 0.03031 0.73963 0.74126 0.49539 0.49777 150 150 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   China 2022-04-12 Adult Adult Gonad Reproductive System
69481 69481 SRR18731942 SRX14832177 SRS12587950 SRP369810 PRJNA825753 Zebrafish ovarian transcriptome sequencing PRJNA825753 Other 1 The transcriptome sequencing of nine samples was completed and a total of 63.66 Gb Clean Data was obtained and the Clean Data of each sample reached 6.14 Gb and above and the percentage of Q30 bases was 91.64 % and above.2 The Clean Reads of each sample were compared with the designated reference genome for sequence alignment and the alignment efficiency was 92.04% or more.3 Functional annotation was performed for all genes including comparison with NR [1] Swiss Prot [2] KEGG [3] COG [4] KOG [5] GO [6] and Pfam databases and annotation results for a total of 26929 genes were obtained.4 Based on the comparison results the libraries were evaluated.5 Based on the comparison results gene expression analysis was performed. Based on the expression of genes in different samples differentially expressed genes were identified and functional annotation and enrichment analysis were performed.         Zebrafish Ovaries   strain:not applicable|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|age:6 Months|dev stage:adult fish|sex:female|tissue:Ovaries|BioSampleModel:Model organism or animal                 Adult zebrafish ovary 20220412 7 20220412 7 materials and methods     RNA-Seq TRANSCRIPTOMIC Oligo-dT PAIRED ILLUMINA Illumina HiSeq 2500   SRP369810     GPR19-11-Rescue-1_BKDL202619368-1a-N707-AK417_1.fq.gz GPR19-11-Rescue-1_BKDL202619368-1a-N707-AK417_2.fq.gz fastq fastq 6879986100.0 22933287.0 GPR19 11 Rescue 1 BKDL202619368 1a N707 AK417 1.fq.gz 0:150 1:150 A:1836018740;C:1600279556;G:1633403531;T:1810257249;N:27024 150 150     1836018740 1600279556 1633403531 1810257249 27024 SRX14832177 SRS12587950 SRA1402797 zhejiang ocean university|school of fishery zhejiang ocean university 2 0.94532 0.94398 0.03079 0.03047 0.74077 0.7417 0.49403 0.49651 150 150 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   China 2022-04-12 Adult Adult Gonad Reproductive System
69482 69482 SRR18731943 SRX14832176 SRS12587950 SRP369810 PRJNA825753 Zebrafish ovarian transcriptome sequencing PRJNA825753 Other 1 The transcriptome sequencing of nine samples was completed and a total of 63.66 Gb Clean Data was obtained and the Clean Data of each sample reached 6.14 Gb and above and the percentage of Q30 bases was 91.64 % and above.2 The Clean Reads of each sample were compared with the designated reference genome for sequence alignment and the alignment efficiency was 92.04% or more.3 Functional annotation was performed for all genes including comparison with NR [1] Swiss Prot [2] KEGG [3] COG [4] KOG [5] GO [6] and Pfam databases and annotation results for a total of 26929 genes were obtained.4 Based on the comparison results the libraries were evaluated.5 Based on the comparison results gene expression analysis was performed. Based on the expression of genes in different samples differentially expressed genes were identified and functional annotation and enrichment analysis were performed.         Zebrafish Ovaries   strain:not applicable|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|age:6 Months|dev stage:adult fish|sex:female|tissue:Ovaries|BioSampleModel:Model organism or animal                 Adult zebrafish ovary 20220412 6 20220412 6 materials and methods     RNA-Seq TRANSCRIPTOMIC Oligo-dT PAIRED ILLUMINA Illumina HiSeq 2500   SRP369810     GPR19-11-Treantment-3_BKDL202619368-1a-N706-N508_1.fq.gz GPR19-11-Treantment-3_BKDL202619368-1a-N706-N508_2.fq.gz fastq fastq 7311039900.0 24370133.0 GPR19 11 Treantment 3 BKDL202619368 1a N706 N508 1.fq.gz 0:150 1:150 A:1956990026;C:1696385126;G:1732778037;T:1924857608;N:29103 150 150     1956990026 1696385126 1732778037 1924857608 29103 SRX14832176 SRS12587950 SRA1402797 zhejiang ocean university|school of fishery zhejiang ocean university 2 0.94368 0.94282 0.02754 0.02729 0.74663 0.74811 0.49702 0.4955 150 150 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   China 2022-04-12 Adult Adult Gonad Reproductive System
69483 69483 SRR18731944 SRX14832175 SRS12587950 SRP369810 PRJNA825753 Zebrafish ovarian transcriptome sequencing PRJNA825753 Other 1 The transcriptome sequencing of nine samples was completed and a total of 63.66 Gb Clean Data was obtained and the Clean Data of each sample reached 6.14 Gb and above and the percentage of Q30 bases was 91.64 % and above.2 The Clean Reads of each sample were compared with the designated reference genome for sequence alignment and the alignment efficiency was 92.04% or more.3 Functional annotation was performed for all genes including comparison with NR [1] Swiss Prot [2] KEGG [3] COG [4] KOG [5] GO [6] and Pfam databases and annotation results for a total of 26929 genes were obtained.4 Based on the comparison results the libraries were evaluated.5 Based on the comparison results gene expression analysis was performed. Based on the expression of genes in different samples differentially expressed genes were identified and functional annotation and enrichment analysis were performed.         Zebrafish Ovaries   strain:not applicable|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|age:6 Months|dev stage:adult fish|sex:female|tissue:Ovaries|BioSampleModel:Model organism or animal                 Adult zebrafish ovary 20220412 5 20220412 5 materials and methods     RNA-Seq TRANSCRIPTOMIC Oligo-dT PAIRED ILLUMINA Illumina HiSeq 2500   SRP369810     GPR19-11-Treantment-2_BKDL202619368-1a-N705-N507_1.fq.gz GPR19-11-Treantment-2_BKDL202619368-1a-N705-N507_2.fq.gz fastq fastq 7339647900.0 24465493.0 GPR19 11 Treantment 2 BKDL202619368 1a N705 N507 1.fq.gz 0:150 1:150 A:1968354751;C:1700662595;G:1735948919;T:1934652282;N:29353 150 150     1968354751 1700662595 1735948919 1934652282 29353 SRX14832175 SRS12587950 SRA1402797 zhejiang ocean university|school of fishery zhejiang ocean university 2 0.94395 0.94269 0.02732 0.02717 0.74789 0.74937 0.49089 0.49494 150 150 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   China 2022-04-12 Adult Adult Gonad Reproductive System
69484 69484 SRR18731945 SRX14832174 SRS12587950 SRP369810 PRJNA825753 Zebrafish ovarian transcriptome sequencing PRJNA825753 Other 1 The transcriptome sequencing of nine samples was completed and a total of 63.66 Gb Clean Data was obtained and the Clean Data of each sample reached 6.14 Gb and above and the percentage of Q30 bases was 91.64 % and above.2 The Clean Reads of each sample were compared with the designated reference genome for sequence alignment and the alignment efficiency was 92.04% or more.3 Functional annotation was performed for all genes including comparison with NR [1] Swiss Prot [2] KEGG [3] COG [4] KOG [5] GO [6] and Pfam databases and annotation results for a total of 26929 genes were obtained.4 Based on the comparison results the libraries were evaluated.5 Based on the comparison results gene expression analysis was performed. Based on the expression of genes in different samples differentially expressed genes were identified and functional annotation and enrichment analysis were performed.         Zebrafish Ovaries   strain:not applicable|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|age:6 Months|dev stage:adult fish|sex:female|tissue:Ovaries|BioSampleModel:Model organism or animal                 Adult zebrafish ovary 20220412 4 20220412 4 materials and methods     RNA-Seq TRANSCRIPTOMIC Oligo-dT PAIRED ILLUMINA Illumina HiSeq 2500   SRP369810     GPR19-11-Treantment-1_BKDL202619368-1a-N704-N506_1.fq.gz GPR19-11-Treantment-1_BKDL202619368-1a-N704-N506_2.fq.gz fastq fastq 6671009100.0 22236697.0 GPR19 11 Treantment 1 BKDL202619368 1a N704 N506 1.fq.gz 0:150 1:150 A:1783546565;C:1548532134;G:1583055468;T:1755848772;N:26161 150 150     1783546565 1548532134 1583055468 1755848772 26161 SRX14832174 SRS12587950 SRA1402797 zhejiang ocean university|school of fishery zhejiang ocean university 2 0.94438 0.94175 0.02733 0.02674 0.7487 0.75035 0.49731 0.49703 150 150 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   China 2022-04-12 Adult Adult Gonad Reproductive System
69485 69485 SRR18731946 SRX14832173 SRS12587950 SRP369810 PRJNA825753 Zebrafish ovarian transcriptome sequencing PRJNA825753 Other 1 The transcriptome sequencing of nine samples was completed and a total of 63.66 Gb Clean Data was obtained and the Clean Data of each sample reached 6.14 Gb and above and the percentage of Q30 bases was 91.64 % and above.2 The Clean Reads of each sample were compared with the designated reference genome for sequence alignment and the alignment efficiency was 92.04% or more.3 Functional annotation was performed for all genes including comparison with NR [1] Swiss Prot [2] KEGG [3] COG [4] KOG [5] GO [6] and Pfam databases and annotation results for a total of 26929 genes were obtained.4 Based on the comparison results the libraries were evaluated.5 Based on the comparison results gene expression analysis was performed. Based on the expression of genes in different samples differentially expressed genes were identified and functional annotation and enrichment analysis were performed.         Zebrafish Ovaries   strain:not applicable|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|age:6 Months|dev stage:adult fish|sex:female|tissue:Ovaries|BioSampleModel:Model organism or animal                 Adult zebrafish ovary 20220412 3 20220412 3 materials and methods     RNA-Seq TRANSCRIPTOMIC Oligo-dT PAIRED ILLUMINA Illumina HiSeq 2500   SRP369810     GPR19-11-Control-3_BKDL202619368-1a-N703-N505_1.fq.gz GPR19-11-Control-3_BKDL202619368-1a-N703-N505_2.fq.gz fastq fastq 7449711300.0 24832371.0 GPR19 11 Control 3 BKDL202619368 1a N703 N505 1.fq.gz 0:150 1:150 A:1983055279;C:1737773499;G:1771296356;T:1957556812;N:29354 150 150     1983055279 1737773499 1771296356 1957556812 29354 SRX14832173 SRS12587950 SRA1402797 zhejiang ocean university|school of fishery zhejiang ocean university 2 0.94654 0.9438 0.03145 0.03141 0.73541 0.73746 0.4947 0.49294 150 150 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   China 2022-04-12 Adult Adult Gonad Reproductive System
69486 69486 SRR18731947 SRX14832172 SRS12587950 SRP369810 PRJNA825753 Zebrafish ovarian transcriptome sequencing PRJNA825753 Other 1 The transcriptome sequencing of nine samples was completed and a total of 63.66 Gb Clean Data was obtained and the Clean Data of each sample reached 6.14 Gb and above and the percentage of Q30 bases was 91.64 % and above.2 The Clean Reads of each sample were compared with the designated reference genome for sequence alignment and the alignment efficiency was 92.04% or more.3 Functional annotation was performed for all genes including comparison with NR [1] Swiss Prot [2] KEGG [3] COG [4] KOG [5] GO [6] and Pfam databases and annotation results for a total of 26929 genes were obtained.4 Based on the comparison results the libraries were evaluated.5 Based on the comparison results gene expression analysis was performed. Based on the expression of genes in different samples differentially expressed genes were identified and functional annotation and enrichment analysis were performed.         Zebrafish Ovaries   strain:not applicable|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|age:6 Months|dev stage:adult fish|sex:female|tissue:Ovaries|BioSampleModel:Model organism or animal                 Adult zebrafish ovary 20220412 2 20220412 2 materials and methods     RNA-Seq TRANSCRIPTOMIC Oligo-dT PAIRED ILLUMINA Illumina HiSeq 2500   SRP369810     GPR19-11-Control-2_BKDL202619368-1a-N702-N503_1.fq.gz GPR19-11-Control-2_BKDL202619368-1a-N702-N503_2.fq.gz fastq fastq 7841586000.0 26138620.0 GPR19 11 Control 2 BKDL202619368 1a N702 N503 1.fq.gz 0:150 1:150 A:2087468135;C:1826719181;G:1866580166;T:2060787674;N:30844 150 150     2087468135 1826719181 1866580166 2060787674 30844 SRX14832172 SRS12587950 SRA1402797 zhejiang ocean university|school of fishery zhejiang ocean university 2 0.94595 0.94469 0.03066 0.03015 0.73507 0.73681 0.49237 0.49222 150 150 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   China 2022-04-12 Adult Adult Gonad Reproductive System
69487 69487 SRR18731948 SRX14832171 SRS12587950 SRP369810 PRJNA825753 Zebrafish ovarian transcriptome sequencing PRJNA825753 Other 1 The transcriptome sequencing of nine samples was completed and a total of 63.66 Gb Clean Data was obtained and the Clean Data of each sample reached 6.14 Gb and above and the percentage of Q30 bases was 91.64 % and above.2 The Clean Reads of each sample were compared with the designated reference genome for sequence alignment and the alignment efficiency was 92.04% or more.3 Functional annotation was performed for all genes including comparison with NR [1] Swiss Prot [2] KEGG [3] COG [4] KOG [5] GO [6] and Pfam databases and annotation results for a total of 26929 genes were obtained.4 Based on the comparison results the libraries were evaluated.5 Based on the comparison results gene expression analysis was performed. Based on the expression of genes in different samples differentially expressed genes were identified and functional annotation and enrichment analysis were performed.         Zebrafish Ovaries   strain:not applicable|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|age:6 Months|dev stage:adult fish|sex:female|tissue:Ovaries|BioSampleModel:Model organism or animal                 Adult zebrafish ovary 20220412 1 20220412 1 materials and methods     RNA-Seq TRANSCRIPTOMIC Oligo-dT PAIRED ILLUMINA Illumina HiSeq 2500   SRP369810     GPR19-11-Control-1_BKDL202619368-1a-N701-N502_1.fq.gz GPR19-11-Control-1_BKDL202619368-1a-N701-N502_2.fq.gz fastq fastq 7595890500.0 25319635.0 GPR19 11 Control 1 BKDL202619368 1a N701 N502 1.fq.gz 0:150 1:150 A:2021828878;C:1767048142;G:1812243705;T:1994739951;N:29824 150 150     2021828878 1767048142 1812243705 1994739951 29824 SRX14832171 SRS12587950 SRA1402797 zhejiang ocean university|school of fishery zhejiang ocean university 2 0.94534 0.94354 0.03174 0.0313 0.73519 0.73793 0.49177 0.49256 150 150 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   China 2022-04-12 Adult Adult Gonad Reproductive System
70294 70294 SRR19667654 SRX15717084 SRS13410649 SRP381981 PRJNA849009 Danio rerio strain:AB Raw sequence reads PRJNA849009 Whole Genome Sequencing PG follicles of bmp15 and inha double mutants         inhaMT1   strain:AB|age:4 mpf follicles of bmp15 and inha double mutant fish 1|BioSampleModel:Model organism or animal                 RNAseq of follicle s120 s120 normal RNA Seq of follicles     RNA-Seq TRANSCRIPTOMIC Oligo-dT PAIRED ILLUMINA Illumina NovaSeq 6000   SRP381981     DM_1_1.fq.gz DM_1_2.fq.gz fastq fastq 6921031800.0 23070106.0 DM 1 1.fq.gz 0:150 1:150 A:1831930082;C:1639790798;G:1637197140;T:1812073277;N:40503 150 150     1831930082 1639790798 1637197140 1812073277 40503 SRX15717084 SRS13410649 SRA1438276 University of Macau|Faculty of Health Sciences University of Macau 2 0.92614 0.92573 0.02965 0.02957 0.72766 0.72801 0.50714 0.50825 150 150 B B biological fallback assumption illumina novaseq_era unknown poly_a unknown bulk unknown unknown   China 2022-06-16 Adult Adult Gonad Reproductive System
70295 70295 SRR19667655 SRX15717083 SRS13410648 SRP381981 PRJNA849009 Danio rerio strain:AB Raw sequence reads PRJNA849009 Whole Genome Sequencing PG follicles of bmp15 and inha double mutants         DM 3   strain:AB|age:4 mpf follicles of inha deficient fish 3|BioSampleModel:Model organism or animal                 RNAseq of follicle s119 s119 normal RNA Seq of follicles     RNA-Seq TRANSCRIPTOMIC Oligo-dT PAIRED ILLUMINA Illumina NovaSeq 6000   SRP381981     inhaMT3_1.fq.gz inhaMT3_2.fq.gz fastq fastq 6573034200.0 21910114.0 inhaMT3 1.fq.gz 0:150 1:150 A:1760940385;C:1536777287;G:1533589773;T:1741648733;N:78022 150 150     1760940385 1536777287 1533589773 1741648733 78022 SRX15717083 SRS13410648 SRA1438276 University of Macau|Faculty of Health Sciences University of Macau 2 0.93016 0.92917 0.03919 0.03905 0.7189 0.71894 0.51609 0.51817 150 150 B B biological fallback assumption illumina novaseq_era unknown poly_a unknown bulk unknown unknown   China 2022-06-16 Adult Adult Gonad Reproductive System
70296 70296 SRR19667656 SRX15717082 SRS13410647 SRP381981 PRJNA849009 Danio rerio strain:AB Raw sequence reads PRJNA849009 Whole Genome Sequencing PG follicles of bmp15 and inha double mutants         DM 2   strain:AB|age:4 mpf follicles of inha deficient fish 2|BioSampleModel:Model organism or animal                 RNAseq of follicle s118 s118 normal RNA Seq of follicles     RNA-Seq TRANSCRIPTOMIC Oligo-dT PAIRED ILLUMINA Illumina NovaSeq 6000   SRP381981     inhaMT2_1.fq.gz inhaMT2_2.fq.gz fastq fastq 6822418200.0 22741394.0 inhaMT2 1.fq.gz 0:150 1:150 A:1840430165;C:1584535027;G:1571889057;T:1825126177;N:437774 150 150     1840430165 1584535027 1571889057 1825126177 437774 SRX15717082 SRS13410647 SRA1438276 University of Macau|Faculty of Health Sciences University of Macau 2 0.93221 0.9312 0.0353 0.03541 0.72543 0.72565 0.51132 0.51053 150 150 B B biological fallback assumption illumina novaseq_era unknown poly_a unknown bulk unknown unknown   China 2022-06-16 Adult Adult Gonad Reproductive System
70297 70297 SRR19667657 SRX15717081 SRS13410646 SRP381981 PRJNA849009 Danio rerio strain:AB Raw sequence reads PRJNA849009 Whole Genome Sequencing PG follicles of bmp15 and inha double mutants         DM 1   strain:AB|age:4 mpf follicles of inha deficient fish 1|BioSampleModel:Model organism or animal                 RNAseq of follicle s117 s117 normal RNA Seq of follicles     RNA-Seq TRANSCRIPTOMIC Oligo-dT PAIRED ILLUMINA Illumina NovaSeq 6000   SRP381981     inhaMT1_1.fq.gz inhaMT1_2.fq.gz fastq fastq 6760944000.0 22536480.0 inhaMT1 1.fq.gz 0:150 1:150 A:1818201563;C:1576471504;G:1562849544;T:1803345958;N:75431 150 150     1818201563 1576471504 1562849544 1803345958 75431 SRX15717081 SRS13410646 SRA1438276 University of Macau|Faculty of Health Sciences University of Macau 2 0.93508 0.93436 0.03864 0.03855 0.71932 0.72046 0.50396 0.50419 150 150 B B biological fallback assumption illumina novaseq_era unknown poly_a unknown bulk unknown unknown   China 2022-06-16 Adult Adult Gonad Reproductive System
70298 70298 SRR19667658 SRX15717080 SRS13410645 SRP381981 PRJNA849009 Danio rerio strain:AB Raw sequence reads PRJNA849009 Whole Genome Sequencing PG follicles of bmp15 and inha double mutants         bmp15MT6   strain:AB|age:4 mpf follicles of bmp15 deficient fish 3|BioSampleModel:Model organism or animal                 RNAseq of follicle s116 s116 normal RNA Seq of follicles     RNA-Seq TRANSCRIPTOMIC Oligo-dT PAIRED ILLUMINA Illumina NovaSeq 6000   SRP381981     bmp15MT6_1.fq.gz bmp15MT6_2.fq.gz fastq fastq 7166094000.0 23886980.0 bmp15MT6 1.fq.gz 0:150 1:150 A:1940181724;C:1661267618;G:1644798361;T:1919623267;N:223030 150 150     1940181724 1661267618 1644798361 1919623267 223030 SRX15717080 SRS13410645 SRA1438276 University of Macau|Faculty of Health Sciences University of Macau 2 0.93416 0.93229 0.02449 0.02416 0.74268 0.74322 0.50126 0.50216 150 150 B B biological fallback assumption illumina novaseq_era unknown poly_a unknown bulk unknown unknown   China 2022-06-16 Adult Adult Gonad Reproductive System
70299 70299 SRR19667659 SRX15717079 SRS13410644 SRP381981 PRJNA849009 Danio rerio strain:AB Raw sequence reads PRJNA849009 Whole Genome Sequencing PG follicles of bmp15 and inha double mutants         bmp15MT5   strain:AB|age:4 mpf follicles of bmp15 deficient fish 2|BioSampleModel:Model organism or animal                 RNAseq of follicle s115 s115 normal RNA Seq of follicles     RNA-Seq TRANSCRIPTOMIC Oligo-dT PAIRED ILLUMINA Illumina NovaSeq 6000   SRP381981     bmp15MT5_1.fq.gz bmp15MT5_2.fq.gz fastq fastq 6803755200.0 22679184.0 bmp15MT5 1.fq.gz 0:150 1:150 A:1805035286;C:1608870778;G:1597859189;T:1791709754;N:280193 150 150     1805035286 1608870778 1597859189 1791709754 280193 SRX15717079 SRS13410644 SRA1438276 University of Macau|Faculty of Health Sciences University of Macau 2 0.93476 0.92086 0.02132 0.02063 0.74278 0.7441 0.49866 0.49457 150 150 B B biological fallback assumption illumina novaseq_era unknown poly_a unknown bulk unknown unknown   China 2022-06-16 Adult Adult Gonad Reproductive System
70300 70300 SRR19667660 SRX15717078 SRS13410643 SRP381981 PRJNA849009 Danio rerio strain:AB Raw sequence reads PRJNA849009 Whole Genome Sequencing PG follicles of bmp15 and inha double mutants         bmp15MT4   strain:AB|age:4 mpf follicles of bmp15 deficient fish 1|BioSampleModel:Model organism or animal                 RNAseq of follicle s114 s114 normal RNA Seq of follicles     RNA-Seq TRANSCRIPTOMIC Oligo-dT PAIRED ILLUMINA Illumina NovaSeq 6000   SRP381981     bmp15MT4_1.fq.gz bmp15MT4_2.fq.gz fastq fastq 7014070800.0 23380236.0 bmp15MT4 1.fq.gz 0:150 1:150 A:1875349504;C:1644372283;G:1635747356;T:1858373832;N:227825 150 150     1875349504 1644372283 1635747356 1858373832 227825 SRX15717078 SRS13410643 SRA1438276 University of Macau|Faculty of Health Sciences University of Macau 2 0.93659 0.93589 0.02241 0.02242 0.74521 0.74576 0.49388 0.48655 150 150 B B biological fallback assumption illumina novaseq_era unknown poly_a unknown bulk unknown unknown   China 2022-06-16 Adult Adult Gonad Reproductive System
70301 70301 SRR19667661 SRX15717077 SRS13410642 SRP381981 PRJNA849009 Danio rerio strain:AB Raw sequence reads PRJNA849009 Whole Genome Sequencing PG follicles of bmp15 and inha double mutants         WT 6   strain:AB|age:4 mpf follicles of wide type fish 3|BioSampleModel:Model organism or animal                 RNAseq of follicle s113 s113 normal RNA Seq of follicles     RNA-Seq TRANSCRIPTOMIC Oligo-dT PAIRED ILLUMINA Illumina NovaSeq 6000   SRP381981     WT_6_1.fq.gz WT_6_2.fq.gz fastq fastq 7248246300.0 24160821.0 WT 6 1.fq.gz 0:150 1:150 A:1935427647;C:1689967967;G:1699538856;T:1923133040;N:178790 150 150     1935427647 1689967967 1699538856 1923133040 178790 SRX15717077 SRS13410642 SRA1438276 University of Macau|Faculty of Health Sciences University of Macau 2 0.9152 0.91232 0.02035 0.02009 0.77096 0.77155 0.52593 0.51868 150 150 B B biological fallback assumption illumina novaseq_era unknown poly_a unknown bulk unknown unknown   China 2022-06-16 Adult Adult Gonad Reproductive System
70302 70302 SRR19667662 SRX15717076 SRS13410641 SRP381981 PRJNA849009 Danio rerio strain:AB Raw sequence reads PRJNA849009 Whole Genome Sequencing PG follicles of bmp15 and inha double mutants         inhaMT3   strain:AB|age:4 mpf follicles of bmp15 and inha double mutant fish 3|BioSampleModel:Model organism or animal                 RNAseq of follicle s122 s122 normal RNA Seq of follicles     RNA-Seq TRANSCRIPTOMIC Oligo-dT PAIRED ILLUMINA Illumina NovaSeq 6000   SRP381981     DM_3_1.fq.gz DM_3_2.fq.gz fastq fastq 6909048600.0 23030162.0 DM 3 1.fq.gz 0:150 1:150 A:1853931653;C:1612612418;G:1608288840;T:1834139693;N:75996 150 150     1853931653 1612612418 1608288840 1834139693 75996 SRX15717076 SRS13410641 SRA1438276 University of Macau|Faculty of Health Sciences University of Macau 2 0.90832 0.92595 0.03399 0.03483 0.72569 0.7246 0.51378 0.51136 150 150 B B biological fallback assumption illumina novaseq_era unknown poly_a unknown bulk unknown unknown   China 2022-06-16 Adult Adult Gonad Reproductive System

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CREATE TABLE run_metadata("run.accession" VARCHAR, "experiment.accession" VARCHAR, "sample.accession" VARCHAR, "study.accession" VARCHAR, bioproject VARCHAR, "study.title" VARCHAR, "study.alias" VARCHAR, "study.type" VARCHAR, "study.abstract" VARCHAR, "study.attributes" VARCHAR, "study.PMIDs" VARCHAR, "sample.description" VARCHAR, "sample.title" VARCHAR, "sample.alias" VARCHAR, "sample.centername" VARCHAR, "sample.attributes" VARCHAR, "GEOsample.title" VARCHAR, "GEOsample.dataprocessing" VARCHAR, "GEOsample.source" VARCHAR, "GEOsample.treatmentprotocol" VARCHAR, "GEOsample.extractprotocol" VARCHAR, "GEOsample.growthprotocol" VARCHAR, "GEOsample.characteristics" VARCHAR, "GEOsample.accession" VARCHAR, "experiment.title" VARCHAR, "experiment.alias" VARCHAR, "experiment.library_name" VARCHAR, "experiment.design_description" VARCHAR, "experiment.library_construction_protocol" VARCHAR, "experiment.attributes" VARCHAR, "experiment.library_strategy" VARCHAR, "experiment.library_source" VARCHAR, "experiment.library_selection" VARCHAR, "experiment.library_layout" VARCHAR, "experiment.platform" VARCHAR, "experiment.instrument_model" VARCHAR, "experiment.spot_descriptor" VARCHAR, "experiment.study_ref" VARCHAR, "run.title" VARCHAR, "run.attributes" VARCHAR, "run.filename" VARCHAR, "run.semantic_name" VARCHAR, "run.total_bases" DOUBLE, "run.total_spots" DOUBLE, "run.alias" VARCHAR, "run.read_lengths" VARCHAR, "run.base_counts" VARCHAR, "run.r1_length" BIGINT, "run.r2_length" BIGINT, "run.r3_length" BIGINT, "run.r4_length" BIGINT, "run.Acount" BIGINT, "run.Ccount" BIGINT, "run.Gcount" BIGINT, "run.Tcount" BIGINT, "run.Ncount" BIGINT, "run.experiment" VARCHAR, "run.pool_member" VARCHAR, "submission.accession" VARCHAR, "submission.srasource" VARCHAR, "submission.bioprojectsource" VARCHAR, "seqdetective.n_mates" BIGINT, "seqdetective.mapping_rate.mate1" DOUBLE, "seqdetective.mapping_rate.mate2" DOUBLE, "seqdetective.nofeature_rate.mate1" DOUBLE, "seqdetective.nofeature_rate.mate2" DOUBLE, "seqdetective.sparsity.mate1" DOUBLE, "seqdetective.sparsity.mate2" DOUBLE, "seqdetective.pos_strand_rate.mate1" DOUBLE, "seqdetective.pos_strand_rate.mate2" DOUBLE, "seqdetective.readlen.mate1" BIGINT, "seqdetective.readlen.mate2" BIGINT, "seqdetective.judgement.mate1" VARCHAR, "seqdetective.judgement.mate2" VARCHAR, "seqdetective.judgement.reason" VARCHAR, platform_family VARCHAR, instrument_generation VARCHAR, read_bias VARCHAR, selection_class VARCHAR, prep_kit VARCHAR, sc_or_bulk VARCHAR, tech_class VARCHAR, technology VARCHAR, tech_variant VARCHAR, "submission.bioprojectsource.country" VARCHAR, earliest_date DATE, devstage_curation VARCHAR, devstage_curation_coarse VARCHAR, tissue_curation VARCHAR, tissue_curation_coarse VARCHAR);;
CREATE INDEX idx_run_bioproject ON run_metadata(bioproject);;
CREATE INDEX idx_run_run_accession ON run_metadata("run.accession");;