run_metadata
54 rows where experiment.library_selection = "Oligo-dT", technology = "smartseq" and tissue_curation = "Brain"
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| Link | rowid ▼ | run.accession | experiment.accession | sample.accession | study.accession | bioproject | study.title | study.alias | study.type | study.abstract | study.attributes | study.PMIDs | sample.description | sample.title | sample.alias | sample.centername | sample.attributes | GEOsample.title | GEOsample.dataprocessing | GEOsample.source | GEOsample.treatmentprotocol | GEOsample.extractprotocol | GEOsample.growthprotocol | GEOsample.characteristics | GEOsample.accession | experiment.title | experiment.alias | experiment.library_name | experiment.design_description | experiment.library_construction_protocol | experiment.attributes | experiment.library_strategy | experiment.library_source | experiment.library_selection | experiment.library_layout | experiment.platform | experiment.instrument_model | experiment.spot_descriptor | experiment.study_ref | run.title | run.attributes | run.filename | run.semantic_name | run.total_bases | run.total_spots | run.alias | run.read_lengths | run.base_counts | run.r1_length | run.r2_length | run.r3_length | run.r4_length | run.Acount | run.Ccount | run.Gcount | run.Tcount | run.Ncount | run.experiment | run.pool_member | submission.accession | submission.srasource | submission.bioprojectsource | seqdetective.n_mates | seqdetective.mapping_rate.mate1 | seqdetective.mapping_rate.mate2 | seqdetective.nofeature_rate.mate1 | seqdetective.nofeature_rate.mate2 | seqdetective.sparsity.mate1 | seqdetective.sparsity.mate2 | seqdetective.pos_strand_rate.mate1 | seqdetective.pos_strand_rate.mate2 | seqdetective.readlen.mate1 | seqdetective.readlen.mate2 | seqdetective.judgement.mate1 | seqdetective.judgement.mate2 | seqdetective.judgement.reason | platform_family | instrument_generation | read_bias | selection_class | prep_kit | sc_or_bulk | tech_class | technology | tech_variant | submission.bioprojectsource.country | earliest_date | devstage_curation | devstage_curation_coarse | tissue_curation | tissue_curation_coarse |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 10237 | 10237 | ERR7131169 | ERX6698608 | ERS8070397 | ERP132560 | PRJEB48218 | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E-MTAB-11083 | Transcriptome Analysis | To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb. | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | F8 Nega | SAMEA10418613 | Friedrich Miescher Institute for Biomedical Research | ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418613|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F8 Nega|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry /GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 8|organism part:olfactory bulb|sample name:E MTAB 11083:F8 Nega|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E MTAB 11083:F8 Nega s | F8 Nega s | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | Experimental Factor: fraction:mCherry /GFP | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP132560 | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | 2874F22-1_210715_D00404_0538_BCD91CANXX_TCGACGTC-CTAAGCCT_L006_R1_001.fastq.gz | fastq | 658093749.0 | 12903799.0 | E MTAB 11083:2874F22 1 210715 D00404 0538 BCD91CANXX TCGACGTC CTAAGCCT L006 | 0:51 1:0 | A:173355262;C:152464129;G:147489024;T:184739885;N:45449 | 51 | 0 | 173355262 | 152464129 | 147489024 | 184739885 | 45449 | ERX6698608 | ERS8070397 | ERA6757553 | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | 1 | 0.78328 | 0.15147 | 0.71289 | 0.53671 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | poly_a | nextera | sc | single_cell_plate | smartseq | Switzerland | 2021-12-01 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||
| 10238 | 10238 | ERR7131170 | ERX6698608 | ERS8070397 | ERP132560 | PRJEB48218 | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E-MTAB-11083 | Transcriptome Analysis | To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb. | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | F8 Nega | SAMEA10418613 | Friedrich Miescher Institute for Biomedical Research | ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418613|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F8 Nega|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry /GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 8|organism part:olfactory bulb|sample name:E MTAB 11083:F8 Nega|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E MTAB 11083:F8 Nega s | F8 Nega s | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | Experimental Factor: fraction:mCherry /GFP | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP132560 | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | 2874F22-2_210715_D00404_0538_BCD91CANXX_TCGACGTC-CTAAGCCT_L007_R1_001.fastq.gz | fastq | 661103769.0 | 12962819.0 | E MTAB 11083:2874F22 2 210715 D00404 0538 BCD91CANXX TCGACGTC CTAAGCCT L007 | 0:51 1:0 | A:174223249;C:153223284;G:148276805;T:185335092;N:45339 | 51 | 0 | 174223249 | 153223284 | 148276805 | 185335092 | 45339 | ERX6698608 | ERS8070397 | ERA6757553 | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | 1 | 0.78539 | 0.15054 | 0.70897 | 0.5322 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | poly_a | nextera | sc | single_cell_plate | smartseq | Switzerland | 2021-12-01 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||
| 10239 | 10239 | ERR7131167 | ERX6698607 | ERS8070396 | ERP132560 | PRJEB48218 | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E-MTAB-11083 | Transcriptome Analysis | To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb. | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | F8 mCherry GFP | SAMEA10418612 | Friedrich Miescher Institute for Biomedical Research | ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418612|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F8 mCherry GFP|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP+|genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 8|organism part:olfactory bulb|sample name:E MTAB 11083:F8 mCherry GFP|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E MTAB 11083:F8 mCherry GFP s | F8 mCherry GFP s | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | Experimental Factor: fraction:mCherry+/GFP+ | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP132560 | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | 2874F24-1_210715_D00404_0538_BCD91CANXX_TCGACGTC-TCTCTCCG_L006_R1_001.fastq.gz | fastq | 693183636.0 | 13591836.0 | E MTAB 11083:2874F24 1 210715 D00404 0538 BCD91CANXX TCGACGTC TCTCTCCG L006 | 0:51 1:0 | A:183977687;C:159275153;G:153149826;T:196732022;N:48948 | 51 | 0 | 183977687 | 159275153 | 153149826 | 196732022 | 48948 | ERX6698607 | ERS8070396 | ERA6757553 | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | 1 | 0.68942 | 0.15593 | 0.75828 | 0.51858 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | poly_a | nextera | sc | single_cell_plate | smartseq | Switzerland | 2021-12-01 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||
| 10240 | 10240 | ERR7131168 | ERX6698607 | ERS8070396 | ERP132560 | PRJEB48218 | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E-MTAB-11083 | Transcriptome Analysis | To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb. | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | F8 mCherry GFP | SAMEA10418612 | Friedrich Miescher Institute for Biomedical Research | ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418612|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F8 mCherry GFP|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP+|genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 8|organism part:olfactory bulb|sample name:E MTAB 11083:F8 mCherry GFP|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E MTAB 11083:F8 mCherry GFP s | F8 mCherry GFP s | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | Experimental Factor: fraction:mCherry+/GFP+ | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP132560 | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | 2874F24-2_210715_D00404_0538_BCD91CANXX_TCGACGTC-TCTCTCCG_L007_R1_001.fastq.gz | fastq | 696648678.0 | 13659778.0 | E MTAB 11083:2874F24 2 210715 D00404 0538 BCD91CANXX TCGACGTC TCTCTCCG L007 | 0:51 1:0 | A:184971832;C:160126524;G:154012023;T:197490098;N:48201 | 51 | 0 | 184971832 | 160126524 | 154012023 | 197490098 | 48201 | ERX6698607 | ERS8070396 | ERA6757553 | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | 1 | 0.6906 | 0.15634 | 0.75909 | 0.51346 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | poly_a | nextera | sc | single_cell_plate | smartseq | Switzerland | 2021-12-01 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||
| 10241 | 10241 | ERR7131165 | ERX6698606 | ERS8070395 | ERP132560 | PRJEB48218 | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E-MTAB-11083 | Transcriptome Analysis | To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb. | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | F8 mCherry | SAMEA10418611 | Friedrich Miescher Institute for Biomedical Research | ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418611|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F8 mCherry|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 8|organism part:olfactory bulb|sample name:E MTAB 11083:F8 mCherry|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E MTAB 11083:F8 mCherry s | F8 mCherry s | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | Experimental Factor: fraction:mCherry+/GFP | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP132560 | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | 2874F23-1_210715_D00404_0538_BCD91CANXX_TCGACGTC-CGTCTAAT_L006_R1_001.fastq.gz | fastq | 667760646.0 | 13093346.0 | E MTAB 11083:2874F23 1 210715 D00404 0538 BCD91CANXX TCGACGTC CGTCTAAT L006 | 0:51 1:0 | A:179948739;C:150541918;G:145038845;T:192184092;N:47052 | 51 | 0 | 179948739 | 150541918 | 145038845 | 192184092 | 47052 | ERX6698606 | ERS8070395 | ERA6757553 | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | 1 | 0.71661 | 0.19774 | 0.74576 | 0.52117 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | poly_a | nextera | sc | single_cell_plate | smartseq | Switzerland | 2021-12-01 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||
| 10242 | 10242 | ERR7131166 | ERX6698606 | ERS8070395 | ERP132560 | PRJEB48218 | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E-MTAB-11083 | Transcriptome Analysis | To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb. | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | F8 mCherry | SAMEA10418611 | Friedrich Miescher Institute for Biomedical Research | ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418611|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F8 mCherry|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 8|organism part:olfactory bulb|sample name:E MTAB 11083:F8 mCherry|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E MTAB 11083:F8 mCherry s | F8 mCherry s | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | Experimental Factor: fraction:mCherry+/GFP | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP132560 | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | 2874F23-2_210715_D00404_0538_BCD91CANXX_TCGACGTC-CGTCTAAT_L007_R1_001.fastq.gz | fastq | 670970484.0 | 13156284.0 | E MTAB 11083:2874F23 2 210715 D00404 0538 BCD91CANXX TCGACGTC CGTCTAAT L007 | 0:51 1:0 | A:180906557;C:151329492;G:145816347;T:192872804;N:45284 | 51 | 0 | 180906557 | 151329492 | 145816347 | 192872804 | 45284 | ERX6698606 | ERS8070395 | ERA6757553 | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | 1 | 0.71682 | 0.19902 | 0.74517 | 0.52618 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | poly_a | nextera | sc | single_cell_plate | smartseq | Switzerland | 2021-12-01 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||
| 10243 | 10243 | ERR7131163 | ERX6698605 | ERS8070394 | ERP132560 | PRJEB48218 | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E-MTAB-11083 | Transcriptome Analysis | To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb. | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | F7 Nega | SAMEA10418610 | Friedrich Miescher Institute for Biomedical Research | ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418610|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F7 Nega|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry /GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 7|organism part:olfactory bulb|sample name:E MTAB 11083:F7 Nega|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E MTAB 11083:F7 Nega s | F7 Nega s | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | Experimental Factor: fraction:mCherry /GFP | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP132560 | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | 2874F19-1_210715_D00404_0538_BCD91CANXX_TCGACGTC-GTAAGGAG_L006_R1_001.fastq.gz | fastq | 652205238.0 | 12788338.0 | E MTAB 11083:2874F19 1 210715 D00404 0538 BCD91CANXX TCGACGTC GTAAGGAG L006 | 0:51 1:0 | A:173730405;C:149044598;G:145436777;T:183946928;N:46530 | 51 | 0 | 173730405 | 149044598 | 145436777 | 183946928 | 46530 | ERX6698605 | ERS8070394 | ERA6757553 | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | 1 | 0.80595 | 0.17309 | 0.71003 | 0.53696 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | poly_a | nextera | sc | single_cell_plate | smartseq | Switzerland | 2021-12-01 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||
| 10244 | 10244 | ERR7131164 | ERX6698605 | ERS8070394 | ERP132560 | PRJEB48218 | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E-MTAB-11083 | Transcriptome Analysis | To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb. | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | F7 Nega | SAMEA10418610 | Friedrich Miescher Institute for Biomedical Research | ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418610|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F7 Nega|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry /GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 7|organism part:olfactory bulb|sample name:E MTAB 11083:F7 Nega|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E MTAB 11083:F7 Nega s | F7 Nega s | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | Experimental Factor: fraction:mCherry /GFP | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP132560 | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | 2874F19-2_210715_D00404_0538_BCD91CANXX_TCGACGTC-GTAAGGAG_L007_R1_001.fastq.gz | fastq | 655593423.0 | 12854773.0 | E MTAB 11083:2874F19 2 210715 D00404 0538 BCD91CANXX TCGACGTC GTAAGGAG L007 | 0:51 1:0 | A:174715119;C:149844974;G:146250446;T:184737535;N:45349 | 51 | 0 | 174715119 | 149844974 | 146250446 | 184737535 | 45349 | ERX6698605 | ERS8070394 | ERA6757553 | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | 1 | 0.80585 | 0.17497 | 0.71078 | 0.53767 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | poly_a | nextera | sc | single_cell_plate | smartseq | Switzerland | 2021-12-01 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||
| 10245 | 10245 | ERR7131161 | ERX6698604 | ERS8070393 | ERP132560 | PRJEB48218 | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E-MTAB-11083 | Transcriptome Analysis | To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb. | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | F7 mCherry GFP | SAMEA10418609 | Friedrich Miescher Institute for Biomedical Research | ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418609|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F7 mCherry GFP|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP+|genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 7|organism part:olfactory bulb|sample name:E MTAB 11083:F7 mCherry GFP|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E MTAB 11083:F7 mCherry GFP s | F7 mCherry GFP s | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | Experimental Factor: fraction:mCherry+/GFP+ | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP132560 | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | 2874F21-1_210715_D00404_0538_BCD91CANXX_TCGACGTC-AAGGAGTA_L006_R1_001.fastq.gz | fastq | 661067151.0 | 12962101.0 | E MTAB 11083:2874F21 1 210715 D00404 0538 BCD91CANXX TCGACGTC AAGGAGTA L006 | 0:51 1:0 | A:168281419;C:157701209;G:153487197;T:181550252;N:47074 | 51 | 0 | 168281419 | 157701209 | 153487197 | 181550252 | 47074 | ERX6698604 | ERS8070393 | ERA6757553 | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | 1 | 0.39074 | 0.11592 | 0.82615 | 0.5252 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | poly_a | nextera | sc | single_cell_plate | smartseq | Switzerland | 2021-12-01 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||
| 10246 | 10246 | ERR7131162 | ERX6698604 | ERS8070393 | ERP132560 | PRJEB48218 | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E-MTAB-11083 | Transcriptome Analysis | To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb. | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | F7 mCherry GFP | SAMEA10418609 | Friedrich Miescher Institute for Biomedical Research | ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418609|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F7 mCherry GFP|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP+|genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 7|organism part:olfactory bulb|sample name:E MTAB 11083:F7 mCherry GFP|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E MTAB 11083:F7 mCherry GFP s | F7 mCherry GFP s | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | Experimental Factor: fraction:mCherry+/GFP+ | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP132560 | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | 2874F21-2_210715_D00404_0538_BCD91CANXX_TCGACGTC-AAGGAGTA_L007_R1_001.fastq.gz | fastq | 665709018.0 | 13053118.0 | E MTAB 11083:2874F21 2 210715 D00404 0538 BCD91CANXX TCGACGTC AAGGAGTA L007 | 0:51 1:0 | A:169539998;C:158884603;G:154624803;T:182614264;N:45350 | 51 | 0 | 169539998 | 158884603 | 154624803 | 182614264 | 45350 | ERX6698604 | ERS8070393 | ERA6757553 | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | 1 | 0.38966 | 0.1152 | 0.8258 | 0.53305 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | poly_a | nextera | sc | single_cell_plate | smartseq | Switzerland | 2021-12-01 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||
| 10247 | 10247 | ERR7131159 | ERX6698603 | ERS8070392 | ERP132560 | PRJEB48218 | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E-MTAB-11083 | Transcriptome Analysis | To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb. | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | F7 mCherry | SAMEA10418608 | Friedrich Miescher Institute for Biomedical Research | ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418608|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F7 mCherry|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 7|organism part:olfactory bulb|sample name:E MTAB 11083:F7 mCherry|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E MTAB 11083:F7 mCherry s | F7 mCherry s | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | Experimental Factor: fraction:mCherry+/GFP | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP132560 | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | 2874F20-1_210715_D00404_0538_BCD91CANXX_TCGACGTC-ACTGCATA_L006_R1_001.fastq.gz | fastq | 657813402.0 | 12898302.0 | E MTAB 11083:2874F20 1 210715 D00404 0538 BCD91CANXX TCGACGTC ACTGCATA L006 | 0:51 1:0 | A:175873489;C:149476129;G:143987770;T:188429588;N:46426 | 51 | 0 | 175873489 | 149476129 | 143987770 | 188429588 | 46426 | ERX6698603 | ERS8070392 | ERA6757553 | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | 1 | 0.70507 | 0.2048 | 0.75923 | 0.52828 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | poly_a | nextera | sc | single_cell_plate | smartseq | Switzerland | 2021-12-01 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||
| 10248 | 10248 | ERR7131160 | ERX6698603 | ERS8070392 | ERP132560 | PRJEB48218 | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E-MTAB-11083 | Transcriptome Analysis | To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb. | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | F7 mCherry | SAMEA10418608 | Friedrich Miescher Institute for Biomedical Research | ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418608|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F7 mCherry|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 7|organism part:olfactory bulb|sample name:E MTAB 11083:F7 mCherry|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E MTAB 11083:F7 mCherry s | F7 mCherry s | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | Experimental Factor: fraction:mCherry+/GFP | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP132560 | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | 2874F20-2_210715_D00404_0538_BCD91CANXX_TCGACGTC-ACTGCATA_L007_R1_001.fastq.gz | fastq | 660303426.0 | 12947126.0 | E MTAB 11083:2874F20 2 210715 D00404 0538 BCD91CANXX TCGACGTC ACTGCATA L007 | 0:51 1:0 | A:176607119;C:150065781;G:144613110;T:188972809;N:44607 | 51 | 0 | 176607119 | 150065781 | 144613110 | 188972809 | 44607 | ERX6698603 | ERS8070392 | ERA6757553 | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | 1 | 0.70705 | 0.20314 | 0.75852 | 0.52949 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | poly_a | nextera | sc | single_cell_plate | smartseq | Switzerland | 2021-12-01 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||
| 10249 | 10249 | ERR7131157 | ERX6698602 | ERS8070391 | ERP132560 | PRJEB48218 | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E-MTAB-11083 | Transcriptome Analysis | To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb. | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | F6 Nega | SAMEA10418607 | Friedrich Miescher Institute for Biomedical Research | ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418607|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F6 Nega|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry /GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 6|organism part:olfactory bulb|sample name:E MTAB 11083:F6 Nega|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E MTAB 11083:F6 Nega s | F6 Nega s | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | Experimental Factor: fraction:mCherry /GFP | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP132560 | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | 2874F16-1_210715_D00404_0538_BCD91CANXX_TGCAGCTA-TCTCTCCG_L006_R1_001.fastq.gz | fastq | 669627450.0 | 13129950.0 | E MTAB 11083:2874F16 1 210715 D00404 0538 BCD91CANXX TGCAGCTA TCTCTCCG L006 | 0:51 1:0 | A:177483937;C:154097051;G:148063012;T:189935690;N:47760 | 51 | 0 | 177483937 | 154097051 | 148063012 | 189935690 | 47760 | ERX6698602 | ERS8070391 | ERA6757553 | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | 1 | 0.77183 | 0.16001 | 0.71467 | 0.53352 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | poly_a | nextera | sc | single_cell_plate | smartseq | Switzerland | 2021-12-01 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||
| 10250 | 10250 | ERR7131158 | ERX6698602 | ERS8070391 | ERP132560 | PRJEB48218 | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E-MTAB-11083 | Transcriptome Analysis | To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb. | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | F6 Nega | SAMEA10418607 | Friedrich Miescher Institute for Biomedical Research | ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418607|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F6 Nega|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry /GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 6|organism part:olfactory bulb|sample name:E MTAB 11083:F6 Nega|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E MTAB 11083:F6 Nega s | F6 Nega s | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | Experimental Factor: fraction:mCherry /GFP | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP132560 | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | 2874F16-2_210715_D00404_0538_BCD91CANXX_TGCAGCTA-TCTCTCCG_L007_R1_001.fastq.gz | fastq | 671615073.0 | 13168923.0 | E MTAB 11083:2874F16 2 210715 D00404 0538 BCD91CANXX TGCAGCTA TCTCTCCG L007 | 0:51 1:0 | A:178109157;C:154619024;G:148595207;T:190246469;N:45216 | 51 | 0 | 178109157 | 154619024 | 148595207 | 190246469 | 45216 | ERX6698602 | ERS8070391 | ERA6757553 | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | 1 | 0.77284 | 0.15963 | 0.71569 | 0.53038 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | poly_a | nextera | sc | single_cell_plate | smartseq | Switzerland | 2021-12-01 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||
| 10251 | 10251 | ERR7131155 | ERX6698601 | ERS8070390 | ERP132560 | PRJEB48218 | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E-MTAB-11083 | Transcriptome Analysis | To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb. | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | F6 mCherry GFP | SAMEA10418606 | Friedrich Miescher Institute for Biomedical Research | ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418606|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F6 mCherry GFP|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP+|genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 6|organism part:olfactory bulb|sample name:E MTAB 11083:F6 mCherry GFP|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E MTAB 11083:F6 mCherry GFP s | F6 mCherry GFP s | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | Experimental Factor: fraction:mCherry+/GFP+ | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP132560 | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | 2874F18-1_210715_D00404_0538_BCD91CANXX_TCGACGTC-TATCCTCT_L006_R1_001.fastq.gz | fastq | 667663542.0 | 13091442.0 | E MTAB 11083:2874F18 1 210715 D00404 0538 BCD91CANXX TCGACGTC TATCCTCT L006 | 0:51 1:0 | A:177646483;C:152747528;G:146388226;T:190833951;N:47354 | 51 | 0 | 177646483 | 152747528 | 146388226 | 190833951 | 47354 | ERX6698601 | ERS8070390 | ERA6757553 | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | 1 | 0.67964 | 0.18123 | 0.77193 | 0.53313 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | poly_a | nextera | sc | single_cell_plate | smartseq | Switzerland | 2021-12-01 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||
| 10252 | 10252 | ERR7131156 | ERX6698601 | ERS8070390 | ERP132560 | PRJEB48218 | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E-MTAB-11083 | Transcriptome Analysis | To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb. | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | F6 mCherry GFP | SAMEA10418606 | Friedrich Miescher Institute for Biomedical Research | ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418606|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F6 mCherry GFP|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP+|genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 6|organism part:olfactory bulb|sample name:E MTAB 11083:F6 mCherry GFP|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E MTAB 11083:F6 mCherry GFP s | F6 mCherry GFP s | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | Experimental Factor: fraction:mCherry+/GFP+ | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP132560 | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | 2874F18-2_210715_D00404_0538_BCD91CANXX_TCGACGTC-TATCCTCT_L007_R1_001.fastq.gz | fastq | 670170141.0 | 13140591.0 | E MTAB 11083:2874F18 2 210715 D00404 0538 BCD91CANXX TCGACGTC TATCCTCT L007 | 0:51 1:0 | A:178425395;C:153363855;G:147033779;T:191300976;N:46136 | 51 | 0 | 178425395 | 153363855 | 147033779 | 191300976 | 46136 | ERX6698601 | ERS8070390 | ERA6757553 | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | 1 | 0.67922 | 0.18101 | 0.77141 | 0.53601 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | poly_a | nextera | sc | single_cell_plate | smartseq | Switzerland | 2021-12-01 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||
| 10253 | 10253 | ERR7131153 | ERX6698600 | ERS8070389 | ERP132560 | PRJEB48218 | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E-MTAB-11083 | Transcriptome Analysis | To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb. | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | F6 mCherry | SAMEA10418605 | Friedrich Miescher Institute for Biomedical Research | ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418605|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F6 mCherry|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 6|organism part:olfactory bulb|sample name:E MTAB 11083:F6 mCherry|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E MTAB 11083:F6 mCherry s | F6 mCherry s | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | Experimental Factor: fraction:mCherry+/GFP | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP132560 | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | 2874F17-1_210715_D00404_0538_BCD91CANXX_TCGACGTC-CTCTCTAT_L006_R1_001.fastq.gz | fastq | 677617008.0 | 13286608.0 | E MTAB 11083:2874F17 1 210715 D00404 0538 BCD91CANXX TCGACGTC CTCTCTAT L006 | 0:51 1:0 | A:181785899;C:152797687;G:147070676;T:195914808;N:47938 | 51 | 0 | 181785899 | 152797687 | 147070676 | 195914808 | 47938 | ERX6698600 | ERS8070389 | ERA6757553 | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | 1 | 0.68298 | 0.1953 | 0.76292 | 0.5395 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | poly_a | nextera | sc | single_cell_plate | smartseq | Switzerland | 2021-12-01 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||
| 10254 | 10254 | ERR7131154 | ERX6698600 | ERS8070389 | ERP132560 | PRJEB48218 | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E-MTAB-11083 | Transcriptome Analysis | To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb. | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | F6 mCherry | SAMEA10418605 | Friedrich Miescher Institute for Biomedical Research | ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418605|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F6 mCherry|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 6|organism part:olfactory bulb|sample name:E MTAB 11083:F6 mCherry|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E MTAB 11083:F6 mCherry s | F6 mCherry s | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | Experimental Factor: fraction:mCherry+/GFP | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP132560 | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | 2874F17-2_210715_D00404_0538_BCD91CANXX_TCGACGTC-CTCTCTAT_L007_R1_001.fastq.gz | fastq | 679922973.0 | 13331823.0 | E MTAB 11083:2874F17 2 210715 D00404 0538 BCD91CANXX TCGACGTC CTCTCTAT L007 | 0:51 1:0 | A:182567819;C:153380607;G:147589890;T:196337722;N:46935 | 51 | 0 | 182567819 | 153380607 | 147589890 | 196337722 | 46935 | ERX6698600 | ERS8070389 | ERA6757553 | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | 1 | 0.68391 | 0.19726 | 0.76299 | 0.54099 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | poly_a | nextera | sc | single_cell_plate | smartseq | Switzerland | 2021-12-01 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||
| 10255 | 10255 | ERR7131151 | ERX6698599 | ERS8070388 | ERP132560 | PRJEB48218 | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E-MTAB-11083 | Transcriptome Analysis | To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb. | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | F5 Nega | SAMEA10418604 | Friedrich Miescher Institute for Biomedical Research | ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418604|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F5 Nega|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry /GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 5|organism part:olfactory bulb|sample name:E MTAB 11083:F5 Nega|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E MTAB 11083:F5 Nega s | F5 Nega s | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | Experimental Factor: fraction:mCherry /GFP | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP132560 | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | 2874F13-1_210715_D00404_0538_BCD91CANXX_TGCAGCTA-AAGGAGTA_L006_R1_001.fastq.gz | fastq | 620316315.0 | 12163065.0 | E MTAB 11083:2874F13 1 210715 D00404 0538 BCD91CANXX TGCAGCTA AAGGAGTA L006 | 0:51 1:0 | A:165967355;C:141072216;G:136931219;T:176301963;N:43562 | 51 | 0 | 165967355 | 141072216 | 136931219 | 176301963 | 43562 | ERX6698599 | ERS8070388 | ERA6757553 | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | 1 | 0.78924 | 0.17457 | 0.71934 | 0.53563 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | poly_a | nextera | sc | single_cell_plate | smartseq | Switzerland | 2021-12-01 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||
| 10256 | 10256 | ERR7131152 | ERX6698599 | ERS8070388 | ERP132560 | PRJEB48218 | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E-MTAB-11083 | Transcriptome Analysis | To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb. | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | F5 Nega | SAMEA10418604 | Friedrich Miescher Institute for Biomedical Research | ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418604|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F5 Nega|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry /GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 5|organism part:olfactory bulb|sample name:E MTAB 11083:F5 Nega|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E MTAB 11083:F5 Nega s | F5 Nega s | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | Experimental Factor: fraction:mCherry /GFP | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP132560 | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | 2874F13-2_210715_D00404_0538_BCD91CANXX_TGCAGCTA-AAGGAGTA_L007_R1_001.fastq.gz | fastq | 623905338.0 | 12233438.0 | E MTAB 11083:2874F13 2 210715 D00404 0538 BCD91CANXX TGCAGCTA AAGGAGTA L007 | 0:51 1:0 | A:166996253;C:141937637;G:137825206;T:177103504;N:42738 | 51 | 0 | 166996253 | 141937637 | 137825206 | 177103504 | 42738 | ERX6698599 | ERS8070388 | ERA6757553 | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | 1 | 0.78975 | 0.17387 | 0.72153 | 0.54149 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | poly_a | nextera | sc | single_cell_plate | smartseq | Switzerland | 2021-12-01 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||
| 10257 | 10257 | ERR7131149 | ERX6698598 | ERS8070387 | ERP132560 | PRJEB48218 | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E-MTAB-11083 | Transcriptome Analysis | To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb. | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | F5 mCherry GFP | SAMEA10418603 | Friedrich Miescher Institute for Biomedical Research | ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418603|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F5 mCherry GFP|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP+|genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 5|organism part:olfactory bulb|sample name:E MTAB 11083:F5 mCherry GFP|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E MTAB 11083:F5 mCherry GFP s | F5 mCherry GFP s | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | Experimental Factor: fraction:mCherry+/GFP+ | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP132560 | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | 2874F15-1_210715_D00404_0538_BCD91CANXX_TGCAGCTA-CGTCTAAT_L006_R1_001.fastq.gz | fastq | 670154841.0 | 13140291.0 | E MTAB 11083:2874F15 1 210715 D00404 0538 BCD91CANXX TGCAGCTA CGTCTAAT L006 | 0:51 1:0 | A:182457480;C:149175456;G:144548173;T:193927470;N:46262 | 51 | 0 | 182457480 | 149175456 | 144548173 | 193927470 | 46262 | ERX6698598 | ERS8070387 | ERA6757553 | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | 1 | 0.71875 | 0.18671 | 0.76047 | 0.52398 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | poly_a | nextera | sc | single_cell_plate | smartseq | Switzerland | 2021-12-01 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||
| 10258 | 10258 | ERR7131150 | ERX6698598 | ERS8070387 | ERP132560 | PRJEB48218 | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E-MTAB-11083 | Transcriptome Analysis | To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb. | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | F5 mCherry GFP | SAMEA10418603 | Friedrich Miescher Institute for Biomedical Research | ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418603|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F5 mCherry GFP|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP+|genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 5|organism part:olfactory bulb|sample name:E MTAB 11083:F5 mCherry GFP|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E MTAB 11083:F5 mCherry GFP s | F5 mCherry GFP s | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | Experimental Factor: fraction:mCherry+/GFP+ | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP132560 | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | 2874F15-2_210715_D00404_0538_BCD91CANXX_TGCAGCTA-CGTCTAAT_L007_R1_001.fastq.gz | fastq | 674202048.0 | 13219648.0 | E MTAB 11083:2874F15 2 210715 D00404 0538 BCD91CANXX TGCAGCTA CGTCTAAT L007 | 0:51 1:0 | A:183639511;C:150168226;G:145502071;T:194847755;N:44485 | 51 | 0 | 183639511 | 150168226 | 145502071 | 194847755 | 44485 | ERX6698598 | ERS8070387 | ERA6757553 | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | 1 | 0.72111 | 0.18813 | 0.76378 | 0.5258 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | poly_a | nextera | sc | single_cell_plate | smartseq | Switzerland | 2021-12-01 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||
| 10259 | 10259 | ERR7131147 | ERX6698597 | ERS8070386 | ERP132560 | PRJEB48218 | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E-MTAB-11083 | Transcriptome Analysis | To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb. | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | F5 mCherry | SAMEA10418602 | Friedrich Miescher Institute for Biomedical Research | ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418602|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F5 mCherry|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 5|organism part:olfactory bulb|sample name:E MTAB 11083:F5 mCherry|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E MTAB 11083:F5 mCherry s | F5 mCherry s | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | Experimental Factor: fraction:mCherry+/GFP | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP132560 | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | 2874F14-1_210715_D00404_0538_BCD91CANXX_TGCAGCTA-CTAAGCCT_L006_R1_001.fastq.gz | fastq | 615458259.0 | 12067809.0 | E MTAB 11083:2874F14 1 210715 D00404 0538 BCD91CANXX TGCAGCTA CTAAGCCT L006 | 0:51 1:0 | A:165108582;C:139178883;G:134070672;T:177059757;N:40365 | 51 | 0 | 165108582 | 139178883 | 134070672 | 177059757 | 40365 | ERX6698597 | ERS8070386 | ERA6757553 | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | 1 | 0.69017 | 0.1977 | 0.77193 | 0.53352 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | poly_a | nextera | sc | single_cell_plate | smartseq | Switzerland | 2021-12-01 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||
| 10260 | 10260 | ERR7131148 | ERX6698597 | ERS8070386 | ERP132560 | PRJEB48218 | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E-MTAB-11083 | Transcriptome Analysis | To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb. | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | F5 mCherry | SAMEA10418602 | Friedrich Miescher Institute for Biomedical Research | ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418602|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F5 mCherry|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 5|organism part:olfactory bulb|sample name:E MTAB 11083:F5 mCherry|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E MTAB 11083:F5 mCherry s | F5 mCherry s | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | Experimental Factor: fraction:mCherry+/GFP | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP132560 | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | 2874F14-2_210715_D00404_0538_BCD91CANXX_TGCAGCTA-CTAAGCCT_L007_R1_001.fastq.gz | fastq | 619061613.0 | 12138463.0 | E MTAB 11083:2874F14 2 210715 D00404 0538 BCD91CANXX TGCAGCTA CTAAGCCT L007 | 0:51 1:0 | A:166167516;C:140058774;G:134972850;T:177822733;N:39740 | 51 | 0 | 166167516 | 140058774 | 134972850 | 177822733 | 39740 | ERX6698597 | ERS8070386 | ERA6757553 | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | 1 | 0.69118 | 0.20019 | 0.7707 | 0.52174 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | poly_a | nextera | sc | single_cell_plate | smartseq | Switzerland | 2021-12-01 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||
| 10261 | 10261 | ERR7131145 | ERX6698596 | ERS8070385 | ERP132560 | PRJEB48218 | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E-MTAB-11083 | Transcriptome Analysis | To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb. | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | F4 Nega | SAMEA10418601 | Friedrich Miescher Institute for Biomedical Research | ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418601|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F4 Nega|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry /GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 4|organism part:olfactory bulb|sample name:E MTAB 11083:F4 Nega|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E MTAB 11083:F4 Nega s | F4 Nega s | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | Experimental Factor: fraction:mCherry /GFP | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP132560 | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | 2874F10-1_210715_D00404_0538_BCD91CANXX_TGCAGCTA-TATCCTCT_L006_R1_001.fastq.gz | fastq | 652536687.0 | 12794837.0 | E MTAB 11083:2874F10 1 210715 D00404 0538 BCD91CANXX TGCAGCTA TATCCTCT L006 | 0:51 1:0 | A:178033231;C:144661707;G:139876661;T:189921085;N:44003 | 51 | 0 | 178033231 | 144661707 | 139876661 | 189921085 | 44003 | ERX6698596 | ERS8070385 | ERA6757553 | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | 1 | 0.76201 | 0.2508 | 0.71299 | 0.53379 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | poly_a | nextera | sc | single_cell_plate | smartseq | Switzerland | 2021-12-01 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||
| 10262 | 10262 | ERR7131146 | ERX6698596 | ERS8070385 | ERP132560 | PRJEB48218 | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E-MTAB-11083 | Transcriptome Analysis | To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb. | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | F4 Nega | SAMEA10418601 | Friedrich Miescher Institute for Biomedical Research | ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418601|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F4 Nega|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry /GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 4|organism part:olfactory bulb|sample name:E MTAB 11083:F4 Nega|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E MTAB 11083:F4 Nega s | F4 Nega s | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | Experimental Factor: fraction:mCherry /GFP | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP132560 | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | 2874F10-2_210715_D00404_0538_BCD91CANXX_TGCAGCTA-TATCCTCT_L007_R1_001.fastq.gz | fastq | 655278957.0 | 12848607.0 | E MTAB 11083:2874F10 2 210715 D00404 0538 BCD91CANXX TGCAGCTA TATCCTCT L007 | 0:51 1:0 | A:178938002;C:145332613;G:140527756;T:190437189;N:43397 | 51 | 0 | 178938002 | 145332613 | 140527756 | 190437189 | 43397 | ERX6698596 | ERS8070385 | ERA6757553 | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | 1 | 0.76188 | 0.25356 | 0.71344 | 0.53081 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | poly_a | nextera | sc | single_cell_plate | smartseq | Switzerland | 2021-12-01 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||
| 10263 | 10263 | ERR7131143 | ERX6698595 | ERS8070384 | ERP132560 | PRJEB48218 | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E-MTAB-11083 | Transcriptome Analysis | To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb. | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | F4 mCherry GFP | SAMEA10418600 | Friedrich Miescher Institute for Biomedical Research | ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418600|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F4 mCherry GFP|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP+|genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 4|organism part:olfactory bulb|sample name:E MTAB 11083:F4 mCherry GFP|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E MTAB 11083:F4 mCherry GFP s | F4 mCherry GFP s | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | Experimental Factor: fraction:mCherry+/GFP+ | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP132560 | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | 2874F12-1_210715_D00404_0538_BCD91CANXX_TGCAGCTA-ACTGCATA_L006_R1_001.fastq.gz | fastq | 297921855.0 | 5841605.0 | E MTAB 11083:2874F12 1 210715 D00404 0538 BCD91CANXX TGCAGCTA ACTGCATA L006 | 0:51 1:0 | A:82400724;C:65517496;G:64603058;T:85384480;N:16097 | 51 | 0 | 82400724 | 65517496 | 64603058 | 85384480 | 16097 | ERX6698595 | ERS8070384 | ERA6757553 | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | 1 | 0.72496 | 0.20794 | 0.81223 | 0.52529 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | poly_a | nextera | sc | single_cell_plate | smartseq | Switzerland | 2021-12-01 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||
| 10264 | 10264 | ERR7131144 | ERX6698595 | ERS8070384 | ERP132560 | PRJEB48218 | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E-MTAB-11083 | Transcriptome Analysis | To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb. | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | F4 mCherry GFP | SAMEA10418600 | Friedrich Miescher Institute for Biomedical Research | ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418600|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F4 mCherry GFP|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP+|genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 4|organism part:olfactory bulb|sample name:E MTAB 11083:F4 mCherry GFP|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E MTAB 11083:F4 mCherry GFP s | F4 mCherry GFP s | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | Experimental Factor: fraction:mCherry+/GFP+ | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP132560 | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | 2874F12-2_210715_D00404_0538_BCD91CANXX_TGCAGCTA-ACTGCATA_L007_R1_001.fastq.gz | fastq | 309412971.0 | 6066921.0 | E MTAB 11083:2874F12 2 210715 D00404 0538 BCD91CANXX TGCAGCTA ACTGCATA L007 | 0:51 1:0 | A:85531271;C:68164437;G:67129209;T:88573210;N:14844 | 51 | 0 | 85531271 | 68164437 | 67129209 | 88573210 | 14844 | ERX6698595 | ERS8070384 | ERA6757553 | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | 1 | 0.7245 | 0.20724 | 0.80468 | 0.52791 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | poly_a | nextera | sc | single_cell_plate | smartseq | Switzerland | 2021-12-01 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||
| 10265 | 10265 | ERR7131141 | ERX6698594 | ERS8070383 | ERP132560 | PRJEB48218 | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E-MTAB-11083 | Transcriptome Analysis | To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb. | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | F4 mCherry | SAMEA10418599 | Friedrich Miescher Institute for Biomedical Research | ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418599|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F4 mCherry|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 4|organism part:olfactory bulb|sample name:E MTAB 11083:F4 mCherry|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E MTAB 11083:F4 mCherry s | F4 mCherry s | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | Experimental Factor: fraction:mCherry+/GFP | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP132560 | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | 2874F11-1_210715_D00404_0538_BCD91CANXX_TGCAGCTA-GTAAGGAG_L006_R1_001.fastq.gz | fastq | 672319791.0 | 13182741.0 | E MTAB 11083:2874F11 1 210715 D00404 0538 BCD91CANXX TGCAGCTA GTAAGGAG L006 | 0:51 1:0 | A:181508034;C:150928549;G:146124085;T:193711358;N:47765 | 51 | 0 | 181508034 | 150928549 | 146124085 | 193711358 | 47765 | ERX6698594 | ERS8070383 | ERA6757553 | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | 1 | 0.71726 | 0.19842 | 0.74986 | 0.53363 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | poly_a | nextera | sc | single_cell_plate | smartseq | Switzerland | 2021-12-01 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||
| 10266 | 10266 | ERR7131142 | ERX6698594 | ERS8070383 | ERP132560 | PRJEB48218 | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E-MTAB-11083 | Transcriptome Analysis | To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb. | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | F4 mCherry | SAMEA10418599 | Friedrich Miescher Institute for Biomedical Research | ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418599|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F4 mCherry|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 4|organism part:olfactory bulb|sample name:E MTAB 11083:F4 mCherry|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E MTAB 11083:F4 mCherry s | F4 mCherry s | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | Experimental Factor: fraction:mCherry+/GFP | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP132560 | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | 2874F11-2_210715_D00404_0538_BCD91CANXX_TGCAGCTA-GTAAGGAG_L007_R1_001.fastq.gz | fastq | 675486075.0 | 13244825.0 | E MTAB 11083:2874F11 2 210715 D00404 0538 BCD91CANXX TGCAGCTA GTAAGGAG L007 | 0:51 1:0 | A:182462261;C:151688554;G:146902713;T:194385945;N:46602 | 51 | 0 | 182462261 | 151688554 | 146902713 | 194385945 | 46602 | ERX6698594 | ERS8070383 | ERA6757553 | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | 1 | 0.71745 | 0.19727 | 0.74805 | 0.53092 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | poly_a | nextera | sc | single_cell_plate | smartseq | Switzerland | 2021-12-01 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||
| 10267 | 10267 | ERR7131139 | ERX6698593 | ERS8070382 | ERP132560 | PRJEB48218 | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E-MTAB-11083 | Transcriptome Analysis | To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb. | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | F3 Nega | SAMEA10418598 | Friedrich Miescher Institute for Biomedical Research | ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418598|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F3 Nega|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry /GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 3|organism part:olfactory bulb|sample name:E MTAB 11083:F3 Nega|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E MTAB 11083:F3 Nega s | F3 Nega s | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | Experimental Factor: fraction:mCherry /GFP | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP132560 | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | 2874F7-1_210715_D00404_0538_BCD91CANXX_CGATCAGT-CGTCTAAT_L006_R1_001.fastq.gz | fastq | 630093627.0 | 12354777.0 | E MTAB 11083:2874F7 1 210715 D00404 0538 BCD91CANXX CGATCAGT CGTCTAAT L006 | 0:51 1:0 | A:165132739;C:146179836;G:142796880;T:175940613;N:43559 | 51 | 0 | 165132739 | 146179836 | 142796880 | 175940613 | 43559 | ERX6698593 | ERS8070382 | ERA6757553 | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | 1 | 0.81672 | 0.15219 | 0.71277 | 0.52461 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | poly_a | nextera | sc | single_cell_plate | smartseq | Switzerland | 2021-12-01 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||
| 10268 | 10268 | ERR7131140 | ERX6698593 | ERS8070382 | ERP132560 | PRJEB48218 | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E-MTAB-11083 | Transcriptome Analysis | To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb. | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | F3 Nega | SAMEA10418598 | Friedrich Miescher Institute for Biomedical Research | ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418598|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F3 Nega|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry /GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 3|organism part:olfactory bulb|sample name:E MTAB 11083:F3 Nega|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E MTAB 11083:F3 Nega s | F3 Nega s | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | Experimental Factor: fraction:mCherry /GFP | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP132560 | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | 2874F7-2_210715_D00404_0538_BCD91CANXX_CGATCAGT-CGTCTAAT_L007_R1_001.fastq.gz | fastq | 632305242.0 | 12398142.0 | E MTAB 11083:2874F7 2 210715 D00404 0538 BCD91CANXX CGATCAGT CGTCTAAT L007 | 0:51 1:0 | A:165832174;C:146751895;G:143384539;T:176293313;N:43321 | 51 | 0 | 165832174 | 146751895 | 143384539 | 176293313 | 43321 | ERX6698593 | ERS8070382 | ERA6757553 | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | 1 | 0.81791 | 0.15444 | 0.7151 | 0.52585 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | poly_a | nextera | sc | single_cell_plate | smartseq | Switzerland | 2021-12-01 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||
| 10269 | 10269 | ERR7131137 | ERX6698592 | ERS8070381 | ERP132560 | PRJEB48218 | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E-MTAB-11083 | Transcriptome Analysis | To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb. | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | F3 mCherry GFP | SAMEA10418597 | Friedrich Miescher Institute for Biomedical Research | ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418597|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F3 mCherry GFP|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP+|genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 3|organism part:olfactory bulb|sample name:E MTAB 11083:F3 mCherry GFP|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E MTAB 11083:F3 mCherry GFP s | F3 mCherry GFP s | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | Experimental Factor: fraction:mCherry+/GFP+ | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP132560 | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | 2874F9-1_210715_D00404_0538_BCD91CANXX_TGCAGCTA-CTCTCTAT_L006_R1_001.fastq.gz | fastq | 660379620.0 | 12948620.0 | E MTAB 11083:2874F9 1 210715 D00404 0538 BCD91CANXX TGCAGCTA CTCTCTAT L006 | 0:51 1:0 | A:178482695;C:147417787;G:143797476;T:190635173;N:46489 | 51 | 0 | 178482695 | 147417787 | 143797476 | 190635173 | 46489 | ERX6698592 | ERS8070381 | ERA6757553 | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | 1 | 0.65635 | 0.17778 | 0.76899 | 0.52957 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | poly_a | nextera | sc | single_cell_plate | smartseq | Switzerland | 2021-12-01 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||
| 10270 | 10270 | ERR7131138 | ERX6698592 | ERS8070381 | ERP132560 | PRJEB48218 | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E-MTAB-11083 | Transcriptome Analysis | To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb. | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | F3 mCherry GFP | SAMEA10418597 | Friedrich Miescher Institute for Biomedical Research | ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418597|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F3 mCherry GFP|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP+|genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 3|organism part:olfactory bulb|sample name:E MTAB 11083:F3 mCherry GFP|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E MTAB 11083:F3 mCherry GFP s | F3 mCherry GFP s | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | Experimental Factor: fraction:mCherry+/GFP+ | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP132560 | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | 2874F9-2_210715_D00404_0538_BCD91CANXX_TGCAGCTA-CTCTCTAT_L007_R1_001.fastq.gz | fastq | 662411307.0 | 12988457.0 | E MTAB 11083:2874F9 2 210715 D00404 0538 BCD91CANXX TGCAGCTA CTCTCTAT L007 | 0:51 1:0 | A:179158323;C:147942300;G:144319925;T:190945370;N:45389 | 51 | 0 | 179158323 | 147942300 | 144319925 | 190945370 | 45389 | ERX6698592 | ERS8070381 | ERA6757553 | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | 1 | 0.65813 | 0.17896 | 0.77076 | 0.52835 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | poly_a | nextera | sc | single_cell_plate | smartseq | Switzerland | 2021-12-01 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||
| 10271 | 10271 | ERR7131135 | ERX6698591 | ERS8070380 | ERP132560 | PRJEB48218 | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E-MTAB-11083 | Transcriptome Analysis | To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb. | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | F3 mCherry | SAMEA10418596 | Friedrich Miescher Institute for Biomedical Research | ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418596|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F3 mCherry|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 3|organism part:olfactory bulb|sample name:E MTAB 11083:F3 mCherry|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E MTAB 11083:F3 mCherry s | F3 mCherry s | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | Experimental Factor: fraction:mCherry+/GFP | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP132560 | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | 2874F8-1_210715_D00404_0538_BCD91CANXX_CGATCAGT-TCTCTCCG_L006_R1_001.fastq.gz | fastq | 698608353.0 | 13698203.0 | E MTAB 11083:2874F8 1 210715 D00404 0538 BCD91CANXX CGATCAGT TCTCTCCG L006 | 0:51 1:0 | A:188718612;C:156814081;G:151125083;T:201901570;N:49007 | 51 | 0 | 188718612 | 156814081 | 151125083 | 201901570 | 49007 | ERX6698591 | ERS8070380 | ERA6757553 | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | 1 | 0.70185 | 0.21547 | 0.75588 | 0.53853 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | poly_a | nextera | sc | single_cell_plate | smartseq | Switzerland | 2021-12-01 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||
| 10272 | 10272 | ERR7131136 | ERX6698591 | ERS8070380 | ERP132560 | PRJEB48218 | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E-MTAB-11083 | Transcriptome Analysis | To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb. | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | F3 mCherry | SAMEA10418596 | Friedrich Miescher Institute for Biomedical Research | ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418596|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F3 mCherry|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 3|organism part:olfactory bulb|sample name:E MTAB 11083:F3 mCherry|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E MTAB 11083:F3 mCherry s | F3 mCherry s | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | Experimental Factor: fraction:mCherry+/GFP | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP132560 | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | 2874F8-2_210715_D00404_0538_BCD91CANXX_CGATCAGT-TCTCTCCG_L007_R1_001.fastq.gz | fastq | 699869175.0 | 13722925.0 | E MTAB 11083:2874F8 2 210715 D00404 0538 BCD91CANXX CGATCAGT TCTCTCCG L007 | 0:51 1:0 | A:189192315;C:157176897;G:151498983;T:201952867;N:48113 | 51 | 0 | 189192315 | 157176897 | 151498983 | 201952867 | 48113 | ERX6698591 | ERS8070380 | ERA6757553 | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | 1 | 0.70221 | 0.21684 | 0.75621 | 0.53787 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | poly_a | nextera | sc | single_cell_plate | smartseq | Switzerland | 2021-12-01 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||
| 10273 | 10273 | ERR7131133 | ERX6698590 | ERS8070379 | ERP132560 | PRJEB48218 | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E-MTAB-11083 | Transcriptome Analysis | To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb. | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | F2 Nega | SAMEA10418595 | Friedrich Miescher Institute for Biomedical Research | ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418595|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F2 Nega|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry /GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 2|organism part:olfactory bulb|sample name:E MTAB 11083:F2 Nega|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E MTAB 11083:F2 Nega s | F2 Nega s | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | Experimental Factor: fraction:mCherry /GFP | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP132560 | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | 2874F4-1_210715_D00404_0538_BCD91CANXX_CGATCAGT-ACTGCATA_L006_R1_001.fastq.gz | fastq | 573812985.0 | 11251235.0 | E MTAB 11083:2874F4 1 210715 D00404 0538 BCD91CANXX CGATCAGT ACTGCATA L006 | 0:51 1:0 | A:150716404;C:132203167;G:129732968;T:161120057;N:40389 | 51 | 0 | 150716404 | 132203167 | 129732968 | 161120057 | 40389 | ERX6698590 | ERS8070379 | ERA6757553 | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | 1 | 0.81899 | 0.17302 | 0.72281 | 0.54315 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | poly_a | nextera | sc | single_cell_plate | smartseq | Switzerland | 2021-12-01 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||
| 10274 | 10274 | ERR7131134 | ERX6698590 | ERS8070379 | ERP132560 | PRJEB48218 | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E-MTAB-11083 | Transcriptome Analysis | To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb. | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | F2 Nega | SAMEA10418595 | Friedrich Miescher Institute for Biomedical Research | ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418595|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F2 Nega|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry /GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 2|organism part:olfactory bulb|sample name:E MTAB 11083:F2 Nega|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E MTAB 11083:F2 Nega s | F2 Nega s | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | Experimental Factor: fraction:mCherry /GFP | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP132560 | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | 2874F4-2_210715_D00404_0538_BCD91CANXX_CGATCAGT-ACTGCATA_L007_R1_001.fastq.gz | fastq | 576184587.0 | 11297737.0 | E MTAB 11083:2874F4 2 210715 D00404 0538 BCD91CANXX CGATCAGT ACTGCATA L007 | 0:51 1:0 | A:151387408;C:132788210;G:130392043;T:161577529;N:39397 | 51 | 0 | 151387408 | 132788210 | 130392043 | 161577529 | 39397 | ERX6698590 | ERS8070379 | ERA6757553 | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | 1 | 0.81999 | 0.17082 | 0.72196 | 0.54505 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | poly_a | nextera | sc | single_cell_plate | smartseq | Switzerland | 2021-12-01 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||
| 10275 | 10275 | ERR7131131 | ERX6698589 | ERS8070378 | ERP132560 | PRJEB48218 | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E-MTAB-11083 | Transcriptome Analysis | To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb. | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | F2 mCherry GFP | SAMEA10418594 | Friedrich Miescher Institute for Biomedical Research | ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418594|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F2 mCherry GFP|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP+|genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 2|organism part:olfactory bulb|sample name:E MTAB 11083:F2 mCherry GFP|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E MTAB 11083:F2 mCherry GFP s | F2 mCherry GFP s | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | Experimental Factor: fraction:mCherry+/GFP+ | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP132560 | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | 2874F6-1_210715_D00404_0538_BCD91CANXX_CGATCAGT-CTAAGCCT_L006_R1_001.fastq.gz | fastq | 654811032.0 | 12839432.0 | E MTAB 11083:2874F6 1 210715 D00404 0538 BCD91CANXX CGATCAGT CTAAGCCT L006 | 0:51 1:0 | A:177403486;C:146202733;G:142207545;T:188951373;N:45895 | 51 | 0 | 177403486 | 146202733 | 142207545 | 188951373 | 45895 | ERX6698589 | ERS8070378 | ERA6757553 | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | 1 | 0.67854 | 0.21407 | 0.76104 | 0.52713 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | poly_a | nextera | sc | single_cell_plate | smartseq | Switzerland | 2021-12-01 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||
| 10276 | 10276 | ERR7131132 | ERX6698589 | ERS8070378 | ERP132560 | PRJEB48218 | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E-MTAB-11083 | Transcriptome Analysis | To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb. | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | F2 mCherry GFP | SAMEA10418594 | Friedrich Miescher Institute for Biomedical Research | ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418594|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F2 mCherry GFP|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP+|genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 2|organism part:olfactory bulb|sample name:E MTAB 11083:F2 mCherry GFP|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E MTAB 11083:F2 mCherry GFP s | F2 mCherry GFP s | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | Experimental Factor: fraction:mCherry+/GFP+ | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP132560 | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | 2874F6-2_210715_D00404_0538_BCD91CANXX_CGATCAGT-CTAAGCCT_L007_R1_001.fastq.gz | fastq | 656911926.0 | 12880626.0 | E MTAB 11083:2874F6 2 210715 D00404 0538 BCD91CANXX CGATCAGT CTAAGCCT L007 | 0:51 1:0 | A:178119212;C:146730132;G:142725348;T:189293212;N:44022 | 51 | 0 | 178119212 | 146730132 | 142725348 | 189293212 | 44022 | ERX6698589 | ERS8070378 | ERA6757553 | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | 1 | 0.68162 | 0.21431 | 0.761 | 0.5071 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | poly_a | nextera | sc | single_cell_plate | smartseq | Switzerland | 2021-12-01 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||
| 10277 | 10277 | ERR7131129 | ERX6698588 | ERS8070377 | ERP132560 | PRJEB48218 | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E-MTAB-11083 | Transcriptome Analysis | To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb. | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | F2 mCherry | SAMEA10418593 | Friedrich Miescher Institute for Biomedical Research | ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418593|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F2 mCherry|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 2|organism part:olfactory bulb|sample name:E MTAB 11083:F2 mCherry|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E MTAB 11083:F2 mCherry s | F2 mCherry s | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | Experimental Factor: fraction:mCherry+/GFP | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP132560 | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | 2874F5-1_210715_D00404_0538_BCD91CANXX_CGATCAGT-AAGGAGTA_L006_R1_001.fastq.gz | fastq | 607851558.0 | 11918658.0 | E MTAB 11083:2874F5 1 210715 D00404 0538 BCD91CANXX CGATCAGT AAGGAGTA L006 | 0:51 1:0 | A:164814138;C:136071648;G:132945950;T:173976881;N:42941 | 51 | 0 | 164814138 | 136071648 | 132945950 | 173976881 | 42941 | ERX6698588 | ERS8070377 | ERA6757553 | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | 1 | 0.78967 | 0.18905 | 0.73241 | 0.52347 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | poly_a | nextera | sc | single_cell_plate | smartseq | Switzerland | 2021-12-01 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||
| 10278 | 10278 | ERR7131130 | ERX6698588 | ERS8070377 | ERP132560 | PRJEB48218 | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E-MTAB-11083 | Transcriptome Analysis | To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb. | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | F2 mCherry | SAMEA10418593 | Friedrich Miescher Institute for Biomedical Research | ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418593|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F2 mCherry|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 2|organism part:olfactory bulb|sample name:E MTAB 11083:F2 mCherry|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E MTAB 11083:F2 mCherry s | F2 mCherry s | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | Experimental Factor: fraction:mCherry+/GFP | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP132560 | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | 2874F5-2_210715_D00404_0538_BCD91CANXX_CGATCAGT-AAGGAGTA_L007_R1_001.fastq.gz | fastq | 610931805.0 | 11979055.0 | E MTAB 11083:2874F5 2 210715 D00404 0538 BCD91CANXX CGATCAGT AAGGAGTA L007 | 0:51 1:0 | A:165714775;C:136822925;G:133684294;T:174667814;N:41997 | 51 | 0 | 165714775 | 136822925 | 133684294 | 174667814 | 41997 | ERX6698588 | ERS8070377 | ERA6757553 | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | 1 | 0.78869 | 0.18885 | 0.73156 | 0.51691 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | poly_a | nextera | sc | single_cell_plate | smartseq | Switzerland | 2021-12-01 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||
| 10279 | 10279 | ERR7131127 | ERX6698587 | ERS8070376 | ERP132560 | PRJEB48218 | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E-MTAB-11083 | Transcriptome Analysis | To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb. | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | F1 Nega | SAMEA10418592 | Friedrich Miescher Institute for Biomedical Research | ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418592|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F1 Nega|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry /GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 1|organism part:olfactory bulb|sample name:E MTAB 11083:F1 Nega|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E MTAB 11083:F1 Nega s | F1 Nega s | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | Experimental Factor: fraction:mCherry /GFP | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP132560 | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | 2874F1-1_210715_D00404_0538_BCD91CANXX_CGATCAGT-CTCTCTAT_L006_R1_001.fastq.gz | fastq | 619561872.0 | 12148272.0 | E MTAB 11083:2874F1 1 210715 D00404 0538 BCD91CANXX CGATCAGT CTCTCTAT L006 | 0:51 1:0 | A:164385545;C:141746313;G:138163219;T:175223323;N:43472 | 51 | 0 | 164385545 | 141746313 | 138163219 | 175223323 | 43472 | ERX6698587 | ERS8070376 | ERA6757553 | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | 1 | 0.80258 | 0.17703 | 0.72614 | 0.53483 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | poly_a | nextera | sc | single_cell_plate | smartseq | Switzerland | 2021-12-01 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||
| 10280 | 10280 | ERR7131128 | ERX6698587 | ERS8070376 | ERP132560 | PRJEB48218 | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E-MTAB-11083 | Transcriptome Analysis | To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb. | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | F1 Nega | SAMEA10418592 | Friedrich Miescher Institute for Biomedical Research | ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418592|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F1 Nega|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry /GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 1|organism part:olfactory bulb|sample name:E MTAB 11083:F1 Nega|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E MTAB 11083:F1 Nega s | F1 Nega s | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | Experimental Factor: fraction:mCherry /GFP | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP132560 | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | 2874F1-2_210715_D00404_0538_BCD91CANXX_CGATCAGT-CTCTCTAT_L007_R1_001.fastq.gz | fastq | 621481971.0 | 12185921.0 | E MTAB 11083:2874F1 2 210715 D00404 0538 BCD91CANXX CGATCAGT CTCTCTAT L007 | 0:51 1:0 | A:165030774;C:142231469;G:138651078;T:175527080;N:41570 | 51 | 0 | 165030774 | 142231469 | 138651078 | 175527080 | 41570 | ERX6698587 | ERS8070376 | ERA6757553 | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | 1 | 0.80248 | 0.17699 | 0.726 | 0.53711 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | poly_a | nextera | sc | single_cell_plate | smartseq | Switzerland | 2021-12-01 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||
| 10281 | 10281 | ERR7131125 | ERX6698586 | ERS8070375 | ERP132560 | PRJEB48218 | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E-MTAB-11083 | Transcriptome Analysis | To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb. | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | F1 mCherry GFP | SAMEA10418591 | Friedrich Miescher Institute for Biomedical Research | ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418591|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F1 mCherry GFP|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP+|genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 1|organism part:olfactory bulb|sample name:E MTAB 11083:F1 mCherry GFP|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E MTAB 11083:F1 mCherry GFP s | F1 mCherry GFP s | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | Experimental Factor: fraction:mCherry+/GFP+ | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP132560 | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | 2874F3-1_210715_D00404_0538_BCD91CANXX_CGATCAGT-GTAAGGAG_L006_R1_001.fastq.gz | fastq | 651189369.0 | 12768419.0 | E MTAB 11083:2874F3 1 210715 D00404 0538 BCD91CANXX CGATCAGT GTAAGGAG L006 | 0:51 1:0 | A:176398415;C:145659111;G:142137610;T:186948280;N:45953 | 51 | 0 | 176398415 | 145659111 | 142137610 | 186948280 | 45953 | ERX6698586 | ERS8070375 | ERA6757553 | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | 1 | 0.69561 | 0.18489 | 0.75345 | 0.52316 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | poly_a | nextera | sc | single_cell_plate | smartseq | Switzerland | 2021-12-01 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||
| 10282 | 10282 | ERR7131126 | ERX6698586 | ERS8070375 | ERP132560 | PRJEB48218 | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E-MTAB-11083 | Transcriptome Analysis | To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb. | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | F1 mCherry GFP | SAMEA10418591 | Friedrich Miescher Institute for Biomedical Research | ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418591|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F1 mCherry GFP|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP+|genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 1|organism part:olfactory bulb|sample name:E MTAB 11083:F1 mCherry GFP|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E MTAB 11083:F1 mCherry GFP s | F1 mCherry GFP s | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | Experimental Factor: fraction:mCherry+/GFP+ | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP132560 | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | 2874F3-2_210715_D00404_0538_BCD91CANXX_CGATCAGT-GTAAGGAG_L007_R1_001.fastq.gz | fastq | 653398944.0 | 12811744.0 | E MTAB 11083:2874F3 2 210715 D00404 0538 BCD91CANXX CGATCAGT GTAAGGAG L007 | 0:51 1:0 | A:177139734;C:146171766;G:142707146;T:187335475;N:44823 | 51 | 0 | 177139734 | 146171766 | 142707146 | 187335475 | 44823 | ERX6698586 | ERS8070375 | ERA6757553 | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | 1 | 0.69496 | 0.18414 | 0.75375 | 0.52317 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | poly_a | nextera | sc | single_cell_plate | smartseq | Switzerland | 2021-12-01 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||
| 10283 | 10283 | ERR7131123 | ERX6698585 | ERS8070374 | ERP132560 | PRJEB48218 | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E-MTAB-11083 | Transcriptome Analysis | To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb. | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | F1 mCherry | SAMEA10418590 | Friedrich Miescher Institute for Biomedical Research | ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418590|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F1 mCherry|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 1|organism part:olfactory bulb|sample name:E MTAB 11083:F1 mCherry|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E MTAB 11083:F1 mCherry s | F1 mCherry s | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | Experimental Factor: fraction:mCherry+/GFP | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP132560 | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | 2874F2-1_210715_D00404_0538_BCD91CANXX_CGATCAGT-TATCCTCT_L006_R1_001.fastq.gz | fastq | 613574319.0 | 12030869.0 | E MTAB 11083:2874F2 1 210715 D00404 0538 BCD91CANXX CGATCAGT TATCCTCT L006 | 0:51 1:0 | A:164891629;C:138401759;G:135818073;T:174419828;N:43030 | 51 | 0 | 164891629 | 138401759 | 135818073 | 174419828 | 43030 | ERX6698585 | ERS8070374 | ERA6757553 | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | 1 | 0.79499 | 0.21674 | 0.73359 | 0.52207 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | poly_a | nextera | sc | single_cell_plate | smartseq | Switzerland | 2021-12-01 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||
| 10284 | 10284 | ERR7131124 | ERX6698585 | ERS8070374 | ERP132560 | PRJEB48218 | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E-MTAB-11083 | Transcriptome Analysis | To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb. | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | F1 mCherry | SAMEA10418590 | Friedrich Miescher Institute for Biomedical Research | ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418590|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F1 mCherry|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 1|organism part:olfactory bulb|sample name:E MTAB 11083:F1 mCherry|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E MTAB 11083:F1 mCherry s | F1 mCherry s | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | Experimental Factor: fraction:mCherry+/GFP | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP132560 | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | 2874F2-2_210715_D00404_0538_BCD91CANXX_CGATCAGT-TATCCTCT_L007_R1_001.fastq.gz | fastq | 615666798.0 | 12071898.0 | E MTAB 11083:2874F2 2 210715 D00404 0538 BCD91CANXX CGATCAGT TATCCTCT L007 | 0:51 1:0 | A:165536502;C:138952758;G:136375193;T:174760659;N:41686 | 51 | 0 | 165536502 | 138952758 | 136375193 | 174760659 | 41686 | ERX6698585 | ERS8070374 | ERA6757553 | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | 1 | 0.7941 | 0.21645 | 0.73494 | 0.50761 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | poly_a | nextera | sc | single_cell_plate | smartseq | Switzerland | 2021-12-01 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||
| 33311 | 33311 | SRR29927545 | SRX25421505 | SRS22081661 | SRP521592 | PRJNA1139127 | Laser capture microdissection RNA sequencing of zebrafish optic nerve regeneration | PRJNA1139127 | Other | Zebrafish have the ability to successfully regenerate the connection from the eye to the brain following optic nerve injury in adult. To identify differentially expressed genes in the affected tissues we performed laser capture mRNA sequencing of the retinal ganglion cell layer inner nuclear layer outer nuclear layer and bulk optic nerve in 3 day post optic nerve injury versus naive control animals. The 3 xxx post injury time point was chosen as a time when axons are actively regenerating throughout the optic nerve to the optic chiasm. These data provide a resource for identifying genes expressed in each tissue and their differential expression during optic nerve regeneration. | ON 3d rep3 | MCWR 2019 000157 | strain:wild type|dev stage:adult|collection date:2019 10 18|geo loc name:USA: Wisconsin Milwaukee|sex:female|tissue:optic nerve|treatment:ON 3d rep3|BioSampleModel:Model organism or animal | RNA Seq of Danio rerio: 3 xxx post injury optic nerve | ON 3d rep3 | ON 3d rep3 | SMART Seqv4 | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | PAIRED | ILLUMINA | Illumina HiSeq 2000 | SRP521592 | MCWR_2019_000157_S28_L001_R1_001.fastq.gz MCWR_2019_000157_S28_L001_R2_001.fastq.gz MCWR_2019_000157_S28_L002_R1_001.fastq.gz MCWR_2019_000157_S28_L002_R2_001.fastq.gz | fastq fastq fastq fastq | 12637756180.0 | 41976869.0 | MCWR 2019 000157 S28 L001 R1 001.fastq.gz | 0:150.53 1:150.53 | A:3303913534;C:3007130754;G:3036624621;T:3289934437;N:152834 | 150 | 150 | 3303913534 | 3007130754 | 3036624621 | 3289934437 | 152834 | SRX25421505 | SRS22081661 | SRA1930690 | Medical College of Wisconsin|Cell Biology, Neurobiology, and Anatomy | Medical College of Wisconsin | 2 | 0.9516 | 0.95161 | 0.06268 | 0.06324 | 0.7245 | 0.72539 | 0.50499 | 0.5082 | 151 | 151 | B | B | biological fallback assumption | illumina | hiseq_era | unknown | poly_a | unknown | sc | single_cell_plate | smartseq | United States | 2024-07-23 | Adult | Adult | Brain | Nervous System | ||||||||||||||||||||
| 33312 | 33312 | SRR29927546 | SRX25421504 | SRS22081660 | SRP521592 | PRJNA1139127 | Laser capture microdissection RNA sequencing of zebrafish optic nerve regeneration | PRJNA1139127 | Other | Zebrafish have the ability to successfully regenerate the connection from the eye to the brain following optic nerve injury in adult. To identify differentially expressed genes in the affected tissues we performed laser capture mRNA sequencing of the retinal ganglion cell layer inner nuclear layer outer nuclear layer and bulk optic nerve in 3 day post optic nerve injury versus naive control animals. The 3 xxx post injury time point was chosen as a time when axons are actively regenerating throughout the optic nerve to the optic chiasm. These data provide a resource for identifying genes expressed in each tissue and their differential expression during optic nerve regeneration. | ON 3d rep2 | MCWR 2019 000156 | strain:wild type|dev stage:adult|collection date:2019 10 18|geo loc name:USA: Wisconsin Milwaukee|sex:female|tissue:optic nerve|treatment:ON 3d rep2|BioSampleModel:Model organism or animal | RNA Seq of Danio rerio: 3 xxx post injury optic nerve | ON 3d rep2 | ON 3d rep2 | SMART Seqv4 | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | PAIRED | ILLUMINA | Illumina HiSeq 2000 | SRP521592 | MCWR_2019_000156_S27_L001_R1_001.fastq.gz MCWR_2019_000156_S27_L001_R2_001.fastq.gz MCWR_2019_000156_S27_L002_R1_001.fastq.gz MCWR_2019_000156_S27_L002_R2_001.fastq.gz | fastq fastq fastq fastq | 8225156065.0 | 27320970.0 | MCWR 2019 000156 S27 L001 R1 001.fastq.gz | 0:150.53 1:150.53 | A:2177338510;C:1933476228;G:1950713017;T:2163526872;N:101438 | 150 | 150 | 2177338510 | 1933476228 | 1950713017 | 2163526872 | 101438 | SRX25421504 | SRS22081660 | SRA1930690 | Medical College of Wisconsin|Cell Biology, Neurobiology, and Anatomy | Medical College of Wisconsin | 2 | 0.94816 | 0.94656 | 0.06839 | 0.06869 | 0.71873 | 0.72099 | 0.52048 | 0.51823 | 151 | 151 | B | B | biological fallback assumption | illumina | hiseq_era | unknown | poly_a | unknown | sc | single_cell_plate | smartseq | United States | 2024-07-23 | Adult | Adult | Brain | Nervous System | ||||||||||||||||||||
| 33314 | 33314 | SRR29927548 | SRX25421502 | SRS22081658 | SRP521592 | PRJNA1139127 | Laser capture microdissection RNA sequencing of zebrafish optic nerve regeneration | PRJNA1139127 | Other | Zebrafish have the ability to successfully regenerate the connection from the eye to the brain following optic nerve injury in adult. To identify differentially expressed genes in the affected tissues we performed laser capture mRNA sequencing of the retinal ganglion cell layer inner nuclear layer outer nuclear layer and bulk optic nerve in 3 day post optic nerve injury versus naive control animals. The 3 xxx post injury time point was chosen as a time when axons are actively regenerating throughout the optic nerve to the optic chiasm. These data provide a resource for identifying genes expressed in each tissue and their differential expression during optic nerve regeneration. | ON 3d rep1 | MCWR 2019 000149 | strain:wild type|dev stage:adult|collection date:2019 10 18|geo loc name:USA: Wisconsin Milwaukee|sex:female|tissue:optic nerve|treatment:ON 3d rep1|BioSampleModel:Model organism or animal | RNA Seq of Danio rerio: 3 xxx post injury optic nerve | ON 3d rep1 | ON 3d rep1 | SMART Seqv4 | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | PAIRED | ILLUMINA | Illumina HiSeq 2000 | SRP521592 | MCWR_2019_000149_S20_L001_R1_001.fastq.gz MCWR_2019_000149_S20_L001_R2_001.fastq.gz MCWR_2019_000149_S20_L002_R1_001.fastq.gz MCWR_2019_000149_S20_L002_R2_001.fastq.gz | fastq fastq fastq fastq | 11234251579.0 | 37312508.0 | MCWR 2019 000149 S20 L001 R1 001.fastq.gz | 0:150.54 1:150.55 | A:2884045359;C:2724366727;G:2744442031;T:2881252209;N:145253 | 150 | 150 | 2884045359 | 2724366727 | 2744442031 | 2881252209 | 145253 | SRX25421502 | SRS22081658 | SRA1930690 | Medical College of Wisconsin|Cell Biology, Neurobiology, and Anatomy | Medical College of Wisconsin | 2 | 0.95118 | 0.95151 | 0.09345 | 0.09458 | 0.69589 | 0.6953 | 0.47864 | 0.4794 | 151 | 151 | B | B | biological fallback assumption | illumina | hiseq_era | unknown | poly_a | unknown | sc | single_cell_plate | smartseq | United States | 2024-07-23 | Adult | Adult | Brain | Nervous System | ||||||||||||||||||||
| 33315 | 33315 | SRR29927549 | SRX25421501 | SRS22081657 | SRP521592 | PRJNA1139127 | Laser capture microdissection RNA sequencing of zebrafish optic nerve regeneration | PRJNA1139127 | Other | Zebrafish have the ability to successfully regenerate the connection from the eye to the brain following optic nerve injury in adult. To identify differentially expressed genes in the affected tissues we performed laser capture mRNA sequencing of the retinal ganglion cell layer inner nuclear layer outer nuclear layer and bulk optic nerve in 3 day post optic nerve injury versus naive control animals. The 3 xxx post injury time point was chosen as a time when axons are actively regenerating throughout the optic nerve to the optic chiasm. These data provide a resource for identifying genes expressed in each tissue and their differential expression during optic nerve regeneration. | ON Control rep3 | MCWR 2019 000148 | strain:wild type|dev stage:adult|collection date:2019 10 18|geo loc name:USA: Wisconsin Milwaukee|sex:female|tissue:optic nerve|treatment:ON Control rep3|BioSampleModel:Model organism or animal | RNA Seq of Danio rerio: naive optic nerve | ON Control rep3 | ON Control rep3 | SMART Seqv4 | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | PAIRED | ILLUMINA | Illumina HiSeq 2000 | SRP521592 | MCWR_2019_000148_S19_L002_R2_001.fastq.gz MCWR_2019_000148_S19_L002_R1_001.fastq.gz MCWR_2019_000148_S19_L001_R2_001.fastq.gz MCWR_2019_000148_S19_L001_R1_001.fastq.gz | fastq fastq fastq fastq | 10659326860.0 | 35402192.0 | MCWR 2019 000148 S19 L001 R1 001.fastq.gz | 0:150.55 1:150.55 | A:2756153864;C:2571019186;G:2591020090;T:2740998974;N:134746 | 150 | 150 | 2756153864 | 2571019186 | 2591020090 | 2740998974 | 134746 | SRX25421501 | SRS22081657 | SRA1930690 | Medical College of Wisconsin|Cell Biology, Neurobiology, and Anatomy | Medical College of Wisconsin | 2 | 0.95167 | 0.95091 | 0.09159 | 0.09247 | 0.74852 | 0.74961 | 0.46451 | 0.46354 | 150 | 151 | B | B | biological fallback assumption | illumina | hiseq_era | unknown | poly_a | unknown | sc | single_cell_plate | smartseq | United States | 2024-07-23 | Adult | Adult | Brain | Nervous System | ||||||||||||||||||||
| 33316 | 33316 | SRR29927550 | SRX25421500 | SRS22081656 | SRP521592 | PRJNA1139127 | Laser capture microdissection RNA sequencing of zebrafish optic nerve regeneration | PRJNA1139127 | Other | Zebrafish have the ability to successfully regenerate the connection from the eye to the brain following optic nerve injury in adult. To identify differentially expressed genes in the affected tissues we performed laser capture mRNA sequencing of the retinal ganglion cell layer inner nuclear layer outer nuclear layer and bulk optic nerve in 3 day post optic nerve injury versus naive control animals. The 3 xxx post injury time point was chosen as a time when axons are actively regenerating throughout the optic nerve to the optic chiasm. These data provide a resource for identifying genes expressed in each tissue and their differential expression during optic nerve regeneration. | ON Control rep2 | MCWR 2019 000147 | strain:wild type|dev stage:adult|collection date:2019 10 18|geo loc name:USA: Wisconsin Milwaukee|sex:female|tissue:optic nerve|treatment:ON Control rep2|BioSampleModel:Model organism or animal | RNA Seq of Danio rerio: naive optic nerve | ON Control rep2 | ON Control rep2 | SMART Seqv4 | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | PAIRED | ILLUMINA | Illumina HiSeq 2000 | SRP521592 | MCWR_2019_000147_S18_L002_R2_001.fastq.gz MCWR_2019_000147_S18_L002_R1_001.fastq.gz MCWR_2019_000147_S18_L001_R2_001.fastq.gz MCWR_2019_000147_S18_L001_R1_001.fastq.gz | fastq fastq fastq fastq | 13568087444.0 | 45061061.0 | MCWR 2019 000147 S18 L001 R1 001.fastq.gz | 0:150.55 1:150.55 | A:3431511167;C:3343690230;G:3365044224;T:3427668362;N:173461 | 150 | 150 | 3431511167 | 3343690230 | 3365044224 | 3427668362 | 173461 | SRX25421500 | SRS22081656 | SRA1930690 | Medical College of Wisconsin|Cell Biology, Neurobiology, and Anatomy | Medical College of Wisconsin | 2 | 0.95653 | 0.95547 | 0.08512 | 0.08497 | 0.73093 | 0.73143 | 0.46625 | 0.46899 | 150 | 151 | B | B | biological fallback assumption | illumina | hiseq_era | unknown | poly_a | unknown | sc | single_cell_plate | smartseq | United States | 2024-07-23 | Adult | Adult | Brain | Nervous System | ||||||||||||||||||||
| 33317 | 33317 | SRR29927551 | SRX25421499 | SRS22081655 | SRP521592 | PRJNA1139127 | Laser capture microdissection RNA sequencing of zebrafish optic nerve regeneration | PRJNA1139127 | Other | Zebrafish have the ability to successfully regenerate the connection from the eye to the brain following optic nerve injury in adult. To identify differentially expressed genes in the affected tissues we performed laser capture mRNA sequencing of the retinal ganglion cell layer inner nuclear layer outer nuclear layer and bulk optic nerve in 3 day post optic nerve injury versus naive control animals. The 3 xxx post injury time point was chosen as a time when axons are actively regenerating throughout the optic nerve to the optic chiasm. These data provide a resource for identifying genes expressed in each tissue and their differential expression during optic nerve regeneration. | ON Control rep1 | MCWR 2019 000146 | strain:wild type|dev stage:adult|collection date:2019 10 18|geo loc name:USA: Wisconsin Milwaukee|sex:female|tissue:optic nerve|treatment:ON Control rep1|BioSampleModel:Model organism or animal | RNA Seq of Danio rerio: naive optic nerve | ON Control rep1 | ON Control rep1 | SMART Seqv4 | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | PAIRED | ILLUMINA | Illumina HiSeq 2000 | SRP521592 | MCWR_2019_000146_S17_L002_R2_001.fastq.gz MCWR_2019_000146_S17_L002_R1_001.fastq.gz MCWR_2019_000146_S17_L001_R2_001.fastq.gz MCWR_2019_000146_S17_L001_R1_001.fastq.gz | fastq fastq fastq fastq | 10264964293.0 | 34089023.0 | MCWR 2019 000146 S17 L001 R1 001.fastq.gz | 0:150.56 1:150.56 | A:2551033720;C:2573448189;G:2588121299;T:2552234053;N:127032 | 150 | 150 | 2551033720 | 2573448189 | 2588121299 | 2552234053 | 127032 | SRX25421499 | SRS22081655 | SRA1930690 | Medical College of Wisconsin|Cell Biology, Neurobiology, and Anatomy | Medical College of Wisconsin | 2 | 0.95724 | 0.95715 | 0.07005 | 0.07059 | 0.75637 | 0.75732 | 0.42392 | 0.41384 | 151 | 150 | B | B | biological fallback assumption | illumina | hiseq_era | unknown | poly_a | unknown | sc | single_cell_plate | smartseq | United States | 2024-07-23 | Adult | Adult | Brain | Nervous System |
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CREATE TABLE run_metadata("run.accession" VARCHAR, "experiment.accession" VARCHAR, "sample.accession" VARCHAR, "study.accession" VARCHAR, bioproject VARCHAR, "study.title" VARCHAR, "study.alias" VARCHAR, "study.type" VARCHAR, "study.abstract" VARCHAR, "study.attributes" VARCHAR, "study.PMIDs" VARCHAR, "sample.description" VARCHAR, "sample.title" VARCHAR, "sample.alias" VARCHAR, "sample.centername" VARCHAR, "sample.attributes" VARCHAR, "GEOsample.title" VARCHAR, "GEOsample.dataprocessing" VARCHAR, "GEOsample.source" VARCHAR, "GEOsample.treatmentprotocol" VARCHAR, "GEOsample.extractprotocol" VARCHAR, "GEOsample.growthprotocol" VARCHAR, "GEOsample.characteristics" VARCHAR, "GEOsample.accession" VARCHAR, "experiment.title" VARCHAR, "experiment.alias" VARCHAR, "experiment.library_name" VARCHAR, "experiment.design_description" VARCHAR, "experiment.library_construction_protocol" VARCHAR, "experiment.attributes" VARCHAR, "experiment.library_strategy" VARCHAR, "experiment.library_source" VARCHAR, "experiment.library_selection" VARCHAR, "experiment.library_layout" VARCHAR, "experiment.platform" VARCHAR, "experiment.instrument_model" VARCHAR, "experiment.spot_descriptor" VARCHAR, "experiment.study_ref" VARCHAR, "run.title" VARCHAR, "run.attributes" VARCHAR, "run.filename" VARCHAR, "run.semantic_name" VARCHAR, "run.total_bases" DOUBLE, "run.total_spots" DOUBLE, "run.alias" VARCHAR, "run.read_lengths" VARCHAR, "run.base_counts" VARCHAR, "run.r1_length" BIGINT, "run.r2_length" BIGINT, "run.r3_length" BIGINT, "run.r4_length" BIGINT, "run.Acount" BIGINT, "run.Ccount" BIGINT, "run.Gcount" BIGINT, "run.Tcount" BIGINT, "run.Ncount" BIGINT, "run.experiment" VARCHAR, "run.pool_member" VARCHAR, "submission.accession" VARCHAR, "submission.srasource" VARCHAR, "submission.bioprojectsource" VARCHAR, "seqdetective.n_mates" BIGINT, "seqdetective.mapping_rate.mate1" DOUBLE, "seqdetective.mapping_rate.mate2" DOUBLE, "seqdetective.nofeature_rate.mate1" DOUBLE, "seqdetective.nofeature_rate.mate2" DOUBLE, "seqdetective.sparsity.mate1" DOUBLE, "seqdetective.sparsity.mate2" DOUBLE, "seqdetective.pos_strand_rate.mate1" DOUBLE, "seqdetective.pos_strand_rate.mate2" DOUBLE, "seqdetective.readlen.mate1" BIGINT, "seqdetective.readlen.mate2" BIGINT, "seqdetective.judgement.mate1" VARCHAR, "seqdetective.judgement.mate2" VARCHAR, "seqdetective.judgement.reason" VARCHAR, platform_family VARCHAR, instrument_generation VARCHAR, read_bias VARCHAR, selection_class VARCHAR, prep_kit VARCHAR, sc_or_bulk VARCHAR, tech_class VARCHAR, technology VARCHAR, tech_variant VARCHAR, "submission.bioprojectsource.country" VARCHAR, earliest_date DATE, devstage_curation VARCHAR, devstage_curation_coarse VARCHAR, tissue_curation VARCHAR, tissue_curation_coarse VARCHAR);;
CREATE INDEX idx_run_bioproject ON run_metadata(bioproject);;
CREATE INDEX idx_run_run_accession ON run_metadata("run.accession");;