run_metadata
48 rows where experiment.library_selection = "Oligo-dT", experiment.platform = "ILLUMINA" and tissue_curation = "Eye"
This data as json, CSV (advanced)
| Link | rowid ▼ | run.accession | experiment.accession | sample.accession | study.accession | bioproject | study.title | study.alias | study.type | study.abstract | study.attributes | study.PMIDs | sample.description | sample.title | sample.alias | sample.centername | sample.attributes | GEOsample.title | GEOsample.dataprocessing | GEOsample.source | GEOsample.treatmentprotocol | GEOsample.extractprotocol | GEOsample.growthprotocol | GEOsample.characteristics | GEOsample.accession | experiment.title | experiment.alias | experiment.library_name | experiment.design_description | experiment.library_construction_protocol | experiment.attributes | experiment.library_strategy | experiment.library_source | experiment.library_selection | experiment.library_layout | experiment.platform | experiment.instrument_model | experiment.spot_descriptor | experiment.study_ref | run.title | run.attributes | run.filename | run.semantic_name | run.total_bases | run.total_spots | run.alias | run.read_lengths | run.base_counts | run.r1_length | run.r2_length | run.r3_length | run.r4_length | run.Acount | run.Ccount | run.Gcount | run.Tcount | run.Ncount | run.experiment | run.pool_member | submission.accession | submission.srasource | submission.bioprojectsource | seqdetective.n_mates | seqdetective.mapping_rate.mate1 | seqdetective.mapping_rate.mate2 | seqdetective.nofeature_rate.mate1 | seqdetective.nofeature_rate.mate2 | seqdetective.sparsity.mate1 | seqdetective.sparsity.mate2 | seqdetective.pos_strand_rate.mate1 | seqdetective.pos_strand_rate.mate2 | seqdetective.readlen.mate1 | seqdetective.readlen.mate2 | seqdetective.judgement.mate1 | seqdetective.judgement.mate2 | seqdetective.judgement.reason | platform_family | instrument_generation | read_bias | selection_class | prep_kit | sc_or_bulk | tech_class | technology | tech_variant | submission.bioprojectsource.country | earliest_date | devstage_curation | devstage_curation_coarse | tissue_curation | tissue_curation_coarse |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 10155 | 10155 | ERR5236196 | ERX5039563 | ERS5672247 | ERP126773 | PRJEB42852 | RNA Seq of zebrafish whole eye comparing wild type and mab21l1 c.107delA p.Lys36Argfs*7 embryos at 5 dpf | E-MTAB-10068 | Transcriptome Analysis | Currently there is little knowledge on the functions and signaling pathways of MAB21L1. Therefore we developed a zebrafish genetic model of mab21l1 deficiency to use in order to gain insight into possibly affected pathways and transcripts. We conducted RNA seq on zebrafish whole eyes which were dissected out at 5 dpf Three biological replicates per genotype wild type or homozygous mab21l1 c.107delA were collected with 50 eyes per replicate. RNA was extracted and purified and submitted to Macrogen to conduct RNA Sequencing procedure. | ENA FIRST PUBLIC:2022 01 26|ENA LAST UPDATE:2022 01 26 | Protocols: Whole eyes were dissected from 5 dpf wild type or homozygous mab21l1 c.107delA embryos into 1X Ringer's solution. Three biological samples were collected per genotype and each sample contained 50 eyes. To isolate RNA whole eye samples were lysed and homogenized in TRI Reagent. Then the Direct zol RNA MiniPrep Kit from ZymoResearch was followed to extract and purify RNA. RNA Samples were submitted to Macrogen for RNA Seq performance. Macrogen conducted library prep using a TruSeq Stranded mRNA LT Sample Prep Kit following the TruSeq Stranded mRNA Sample Preparation Guide Part # 15031047 Rev. E. | Zebrafish WT 3 | SAMEA7984965 | Department of Pediatrics and Cell Biology, Neurobiology and Anatomy, The Medical College of Wisconsin, Milwaukee, WI USA | ENA first public:2022 01 26|ENA last update:2022 01 26|External Id:SAMEA7984965|INSDC center alias:Department of Pediatrics and Cell Biology Neurobiology and Anatomy The Medical College of Wisconsin Milwaukee WI USA|INSDC center name:Department of Pediatrics and Cell Biology Neurobiology and Anatomy The Medical College of Wisconsin Milwaukee WI USA|INSDC first public:2022 01 26T00:16:15Z|INSDC last update:2022 01 26T00:16:15Z|INSDC status:public|Submitter Id:E MTAB 10068:Zebrafish WT 3|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:late embryonic stage|genotype:wild type genotype|organism part:eye|sample name:E MTAB 10068:Zebrafish WT 3 | Illumina NovaSeq 6000 paired end sequencing; RNA Seq of zebrafish whole eye comparing wild type and mab21l1 c.107delA p.Lys36Argfs*7 embryos at 5 dpf | E MTAB 10068:Zebrafish WT 3 p | Zebrafish WT 3 p | RNA Seq of zebrafish whole eye comparing wild type and mab21l1 c.107delA p.Lys36Argfs*7 embryos at 5 dpf | Whole eyes were dissected from 5 dpf wild type or homozygous mab21l1 c.107delA embryos into 1X Ringer's solution. Three biological samples were collected per genotype and each sample contained 50 eyes. To isolate RNA whole eye samples were lysed and homogenized in TRI Reagent. Then the Direct zol RNA MiniPrep Kit from ZymoResearch was followed to extract and purify RNA. RNA Samples were submitted to Macrogen for RNA Seq performance. Macrogen conducted library prep using a TruSeq Stranded mRNA LT Sample Prep Kit following the TruSeq Stranded mRNA Sample Preparation Guide Part # 15031047 Rev. E. | Experimental Factor: genotype:wild type genotype | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | ERP126773 | Illumina NovaSeq 6000 paired end sequencing; RNA Seq of zebrafish whole eye comparing wild type and mab21l1 c.107delA p.Lys36Argfs*7 embryos at 5 dpf | ENA FIRST PUBLIC:2022 01 26|ENA LAST UPDATE:2022 01 26 | Zebrafish_WT_3_1.fastq.gz Zebrafish_WT_3_2.fastq.gz | fastq fastq | 7150981594.0 | 23678747.0 | E MTAB 10068:Zebrafish WT 3 | 0:151 1:151 | A:1885053833;C:1690814929;G:1772259046;T:1802798453;N:55333 | 151 | 151 | 1885053833 | 1690814929 | 1772259046 | 1802798453 | 55333 | ERX5039563 | ERS5672247 | ERA3400155 | Department of Pediatrics and Cell Biology, Neurobiology and Anatomy, The Medical College of Wisconsin, Milwaukee, WI USA|European Nucleotide Archive | Department of Pediatrics and Cell Biology, Neurobiology and Anatomy, The Medical College of Wisconsin, Milwaukee, WI USA|European Nucleotide Archive | 2 | 0.95124 | 0.95221 | 0.08338 | 0.08285 | 0.69181 | 0.69256 | 0.46157 | 0.46139 | 151 | 151 | B | B | biological fallback assumption | illumina | novaseq_era | unknown | poly_a | trueseq | bulk | unknown | unknown | United States | 2022-01-26 | Larval | Larval | Eye | Sensory System | |||||||||||||
| 10156 | 10156 | ERR5236195 | ERX5039562 | ERS5672246 | ERP126773 | PRJEB42852 | RNA Seq of zebrafish whole eye comparing wild type and mab21l1 c.107delA p.Lys36Argfs*7 embryos at 5 dpf | E-MTAB-10068 | Transcriptome Analysis | Currently there is little knowledge on the functions and signaling pathways of MAB21L1. Therefore we developed a zebrafish genetic model of mab21l1 deficiency to use in order to gain insight into possibly affected pathways and transcripts. We conducted RNA seq on zebrafish whole eyes which were dissected out at 5 dpf Three biological replicates per genotype wild type or homozygous mab21l1 c.107delA were collected with 50 eyes per replicate. RNA was extracted and purified and submitted to Macrogen to conduct RNA Sequencing procedure. | ENA FIRST PUBLIC:2022 01 26|ENA LAST UPDATE:2022 01 26 | Protocols: Whole eyes were dissected from 5 dpf wild type or homozygous mab21l1 c.107delA embryos into 1X Ringer's solution. Three biological samples were collected per genotype and each sample contained 50 eyes. To isolate RNA whole eye samples were lysed and homogenized in TRI Reagent. Then the Direct zol RNA MiniPrep Kit from ZymoResearch was followed to extract and purify RNA. RNA Samples were submitted to Macrogen for RNA Seq performance. Macrogen conducted library prep using a TruSeq Stranded mRNA LT Sample Prep Kit following the TruSeq Stranded mRNA Sample Preparation Guide Part # 15031047 Rev. E. | Zebrafish WT 2 | SAMEA7984964 | Department of Pediatrics and Cell Biology, Neurobiology and Anatomy, The Medical College of Wisconsin, Milwaukee, WI USA | ENA first public:2022 01 26|ENA last update:2022 01 26|External Id:SAMEA7984964|INSDC center alias:Department of Pediatrics and Cell Biology Neurobiology and Anatomy The Medical College of Wisconsin Milwaukee WI USA|INSDC center name:Department of Pediatrics and Cell Biology Neurobiology and Anatomy The Medical College of Wisconsin Milwaukee WI USA|INSDC first public:2022 01 26T00:16:15Z|INSDC last update:2022 01 26T00:16:15Z|INSDC status:public|Submitter Id:E MTAB 10068:Zebrafish WT 2|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:late embryonic stage|genotype:wild type genotype|organism part:eye|sample name:E MTAB 10068:Zebrafish WT 2 | Illumina NovaSeq 6000 paired end sequencing; RNA Seq of zebrafish whole eye comparing wild type and mab21l1 c.107delA p.Lys36Argfs*7 embryos at 5 dpf | E MTAB 10068:Zebrafish WT 2 p | Zebrafish WT 2 p | RNA Seq of zebrafish whole eye comparing wild type and mab21l1 c.107delA p.Lys36Argfs*7 embryos at 5 dpf | Whole eyes were dissected from 5 dpf wild type or homozygous mab21l1 c.107delA embryos into 1X Ringer's solution. Three biological samples were collected per genotype and each sample contained 50 eyes. To isolate RNA whole eye samples were lysed and homogenized in TRI Reagent. Then the Direct zol RNA MiniPrep Kit from ZymoResearch was followed to extract and purify RNA. RNA Samples were submitted to Macrogen for RNA Seq performance. Macrogen conducted library prep using a TruSeq Stranded mRNA LT Sample Prep Kit following the TruSeq Stranded mRNA Sample Preparation Guide Part # 15031047 Rev. E. | Experimental Factor: genotype:wild type genotype | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | ERP126773 | Illumina NovaSeq 6000 paired end sequencing; RNA Seq of zebrafish whole eye comparing wild type and mab21l1 c.107delA p.Lys36Argfs*7 embryos at 5 dpf | ENA FIRST PUBLIC:2022 01 26|ENA LAST UPDATE:2022 01 26 | Zebrafish_WT_2_1.fastq.gz Zebrafish_WT_2_2.fastq.gz | fastq fastq | 7214044026.0 | 23887563.0 | E MTAB 10068:Zebrafish WT 2 | 0:151 1:151 | A:1902219751;C:1705162605;G:1795933407;T:1810669383;N:58880 | 151 | 151 | 1902219751 | 1705162605 | 1795933407 | 1810669383 | 58880 | ERX5039562 | ERS5672246 | ERA3400155 | Department of Pediatrics and Cell Biology, Neurobiology and Anatomy, The Medical College of Wisconsin, Milwaukee, WI USA|European Nucleotide Archive | Department of Pediatrics and Cell Biology, Neurobiology and Anatomy, The Medical College of Wisconsin, Milwaukee, WI USA|European Nucleotide Archive | 2 | 0.95311 | 0.95386 | 0.07898 | 0.07845 | 0.69045 | 0.69061 | 0.46066 | 0.4628 | 151 | 151 | B | B | biological fallback assumption | illumina | novaseq_era | unknown | poly_a | trueseq | bulk | unknown | unknown | United States | 2022-01-26 | Larval | Larval | Eye | Sensory System | |||||||||||||
| 10157 | 10157 | ERR5236194 | ERX5039561 | ERS5672245 | ERP126773 | PRJEB42852 | RNA Seq of zebrafish whole eye comparing wild type and mab21l1 c.107delA p.Lys36Argfs*7 embryos at 5 dpf | E-MTAB-10068 | Transcriptome Analysis | Currently there is little knowledge on the functions and signaling pathways of MAB21L1. Therefore we developed a zebrafish genetic model of mab21l1 deficiency to use in order to gain insight into possibly affected pathways and transcripts. We conducted RNA seq on zebrafish whole eyes which were dissected out at 5 dpf Three biological replicates per genotype wild type or homozygous mab21l1 c.107delA were collected with 50 eyes per replicate. RNA was extracted and purified and submitted to Macrogen to conduct RNA Sequencing procedure. | ENA FIRST PUBLIC:2022 01 26|ENA LAST UPDATE:2022 01 26 | Protocols: Whole eyes were dissected from 5 dpf wild type or homozygous mab21l1 c.107delA embryos into 1X Ringer's solution. Three biological samples were collected per genotype and each sample contained 50 eyes. To isolate RNA whole eye samples were lysed and homogenized in TRI Reagent. Then the Direct zol RNA MiniPrep Kit from ZymoResearch was followed to extract and purify RNA. RNA Samples were submitted to Macrogen for RNA Seq performance. Macrogen conducted library prep using a TruSeq Stranded mRNA LT Sample Prep Kit following the TruSeq Stranded mRNA Sample Preparation Guide Part # 15031047 Rev. E. | Zebrafish WT 1 | SAMEA7984963 | Department of Pediatrics and Cell Biology, Neurobiology and Anatomy, The Medical College of Wisconsin, Milwaukee, WI USA | ENA first public:2022 01 26|ENA last update:2022 01 26|External Id:SAMEA7984963|INSDC center alias:Department of Pediatrics and Cell Biology Neurobiology and Anatomy The Medical College of Wisconsin Milwaukee WI USA|INSDC center name:Department of Pediatrics and Cell Biology Neurobiology and Anatomy The Medical College of Wisconsin Milwaukee WI USA|INSDC first public:2022 01 26T00:16:15Z|INSDC last update:2022 01 26T00:16:15Z|INSDC status:public|Submitter Id:E MTAB 10068:Zebrafish WT 1|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:late embryonic stage|genotype:wild type genotype|organism part:eye|sample name:E MTAB 10068:Zebrafish WT 1 | Illumina NovaSeq 6000 paired end sequencing; RNA Seq of zebrafish whole eye comparing wild type and mab21l1 c.107delA p.Lys36Argfs*7 embryos at 5 dpf | E MTAB 10068:Zebrafish WT 1 p | Zebrafish WT 1 p | RNA Seq of zebrafish whole eye comparing wild type and mab21l1 c.107delA p.Lys36Argfs*7 embryos at 5 dpf | Whole eyes were dissected from 5 dpf wild type or homozygous mab21l1 c.107delA embryos into 1X Ringer's solution. Three biological samples were collected per genotype and each sample contained 50 eyes. To isolate RNA whole eye samples were lysed and homogenized in TRI Reagent. Then the Direct zol RNA MiniPrep Kit from ZymoResearch was followed to extract and purify RNA. RNA Samples were submitted to Macrogen for RNA Seq performance. Macrogen conducted library prep using a TruSeq Stranded mRNA LT Sample Prep Kit following the TruSeq Stranded mRNA Sample Preparation Guide Part # 15031047 Rev. E. | Experimental Factor: genotype:wild type genotype | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | ERP126773 | Illumina NovaSeq 6000 paired end sequencing; RNA Seq of zebrafish whole eye comparing wild type and mab21l1 c.107delA p.Lys36Argfs*7 embryos at 5 dpf | ENA FIRST PUBLIC:2022 01 26|ENA LAST UPDATE:2022 01 26 | Zebrafish_WT_1_1.fastq.gz Zebrafish_WT_1_2.fastq.gz | fastq fastq | 6632043216.0 | 21960408.0 | E MTAB 10068:Zebrafish WT 1 | 0:151 1:151 | A:1763732625;C:1547925091;G:1636588945;T:1683739270;N:57285 | 151 | 151 | 1763732625 | 1547925091 | 1636588945 | 1683739270 | 57285 | ERX5039561 | ERS5672245 | ERA3400155 | Department of Pediatrics and Cell Biology, Neurobiology and Anatomy, The Medical College of Wisconsin, Milwaukee, WI USA|European Nucleotide Archive | Department of Pediatrics and Cell Biology, Neurobiology and Anatomy, The Medical College of Wisconsin, Milwaukee, WI USA|European Nucleotide Archive | 2 | 0.9495 | 0.95025 | 0.08958 | 0.08856 | 0.68661 | 0.68598 | 0.46029 | 0.46171 | 151 | 151 | B | B | biological fallback assumption | illumina | novaseq_era | unknown | poly_a | trueseq | bulk | unknown | unknown | United States | 2022-01-26 | Larval | Larval | Eye | Sensory System | |||||||||||||
| 10158 | 10158 | ERR5236193 | ERX5039560 | ERS5672244 | ERP126773 | PRJEB42852 | RNA Seq of zebrafish whole eye comparing wild type and mab21l1 c.107delA p.Lys36Argfs*7 embryos at 5 dpf | E-MTAB-10068 | Transcriptome Analysis | Currently there is little knowledge on the functions and signaling pathways of MAB21L1. Therefore we developed a zebrafish genetic model of mab21l1 deficiency to use in order to gain insight into possibly affected pathways and transcripts. We conducted RNA seq on zebrafish whole eyes which were dissected out at 5 dpf Three biological replicates per genotype wild type or homozygous mab21l1 c.107delA were collected with 50 eyes per replicate. RNA was extracted and purified and submitted to Macrogen to conduct RNA Sequencing procedure. | ENA FIRST PUBLIC:2022 01 26|ENA LAST UPDATE:2022 01 26 | Protocols: Whole eyes were dissected from 5 dpf wild type or homozygous mab21l1 c.107delA embryos into 1X Ringer's solution. Three biological samples were collected per genotype and each sample contained 50 eyes. To isolate RNA whole eye samples were lysed and homogenized in TRI Reagent. Then the Direct zol RNA MiniPrep Kit from ZymoResearch was followed to extract and purify RNA. RNA Samples were submitted to Macrogen for RNA Seq performance. Macrogen conducted library prep using a TruSeq Stranded mRNA LT Sample Prep Kit following the TruSeq Stranded mRNA Sample Preparation Guide Part # 15031047 Rev. E. | Zebrafish mab21l1 3 | SAMEA7984962 | Department of Pediatrics and Cell Biology, Neurobiology and Anatomy, The Medical College of Wisconsin, Milwaukee, WI USA | ENA first public:2022 01 26|ENA last update:2022 01 26|External Id:SAMEA7984962|INSDC center alias:Department of Pediatrics and Cell Biology Neurobiology and Anatomy The Medical College of Wisconsin Milwaukee WI USA|INSDC center name:Department of Pediatrics and Cell Biology Neurobiology and Anatomy The Medical College of Wisconsin Milwaukee WI USA|INSDC first public:2022 01 26T00:16:15Z|INSDC last update:2022 01 26T00:16:15Z|INSDC status:public|Submitter Id:E MTAB 10068:Zebrafish mab21l1 3|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:late embryonic stage|genotype:homozygous mab21l1 c.107delA|organism part:eye|sample name:E MTAB 10068:Zebrafish mab21l1 3 | Illumina NovaSeq 6000 paired end sequencing; RNA Seq of zebrafish whole eye comparing wild type and mab21l1 c.107delA p.Lys36Argfs*7 embryos at 5 dpf | E MTAB 10068:Zebrafish mab21l1 3 p | Zebrafish mab21l1 3 p | RNA Seq of zebrafish whole eye comparing wild type and mab21l1 c.107delA p.Lys36Argfs*7 embryos at 5 dpf | Whole eyes were dissected from 5 dpf wild type or homozygous mab21l1 c.107delA embryos into 1X Ringer's solution. Three biological samples were collected per genotype and each sample contained 50 eyes. To isolate RNA whole eye samples were lysed and homogenized in TRI Reagent. Then the Direct zol RNA MiniPrep Kit from ZymoResearch was followed to extract and purify RNA. RNA Samples were submitted to Macrogen for RNA Seq performance. Macrogen conducted library prep using a TruSeq Stranded mRNA LT Sample Prep Kit following the TruSeq Stranded mRNA Sample Preparation Guide Part # 15031047 Rev. E. | Experimental Factor: genotype:homozygous mab21l1 c.107delA | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | ERP126773 | Illumina NovaSeq 6000 paired end sequencing; RNA Seq of zebrafish whole eye comparing wild type and mab21l1 c.107delA p.Lys36Argfs*7 embryos at 5 dpf | ENA FIRST PUBLIC:2022 01 26|ENA LAST UPDATE:2022 01 26 | Zebrafish_mab21l1_3_1.fastq.gz Zebrafish_mab21l1_3_2.fastq.gz | fastq fastq | 6182833618.0 | 20472959.0 | E MTAB 10068:Zebrafish mab21l1 3 | 0:151 1:151 | A:1613191835;C:1471874932;G:1547131522;T:1550575662;N:59667 | 151 | 151 | 1613191835 | 1471874932 | 1547131522 | 1550575662 | 59667 | ERX5039560 | ERS5672244 | ERA3400155 | Department of Pediatrics and Cell Biology, Neurobiology and Anatomy, The Medical College of Wisconsin, Milwaukee, WI USA|European Nucleotide Archive | Department of Pediatrics and Cell Biology, Neurobiology and Anatomy, The Medical College of Wisconsin, Milwaukee, WI USA|European Nucleotide Archive | 2 | 0.95645 | 0.95788 | 0.0697 | 0.06969 | 0.70096 | 0.70033 | 0.43342 | 0.43242 | 151 | 151 | B | B | biological fallback assumption | illumina | novaseq_era | unknown | poly_a | trueseq | bulk | unknown | unknown | United States | 2022-01-26 | Larval | Larval | Eye | Sensory System | |||||||||||||
| 10159 | 10159 | ERR5236192 | ERX5039559 | ERS5672243 | ERP126773 | PRJEB42852 | RNA Seq of zebrafish whole eye comparing wild type and mab21l1 c.107delA p.Lys36Argfs*7 embryos at 5 dpf | E-MTAB-10068 | Transcriptome Analysis | Currently there is little knowledge on the functions and signaling pathways of MAB21L1. Therefore we developed a zebrafish genetic model of mab21l1 deficiency to use in order to gain insight into possibly affected pathways and transcripts. We conducted RNA seq on zebrafish whole eyes which were dissected out at 5 dpf Three biological replicates per genotype wild type or homozygous mab21l1 c.107delA were collected with 50 eyes per replicate. RNA was extracted and purified and submitted to Macrogen to conduct RNA Sequencing procedure. | ENA FIRST PUBLIC:2022 01 26|ENA LAST UPDATE:2022 01 26 | Protocols: Whole eyes were dissected from 5 dpf wild type or homozygous mab21l1 c.107delA embryos into 1X Ringer's solution. Three biological samples were collected per genotype and each sample contained 50 eyes. To isolate RNA whole eye samples were lysed and homogenized in TRI Reagent. Then the Direct zol RNA MiniPrep Kit from ZymoResearch was followed to extract and purify RNA. RNA Samples were submitted to Macrogen for RNA Seq performance. Macrogen conducted library prep using a TruSeq Stranded mRNA LT Sample Prep Kit following the TruSeq Stranded mRNA Sample Preparation Guide Part # 15031047 Rev. E. | Zebrafish mab21l1 2 | SAMEA7984961 | Department of Pediatrics and Cell Biology, Neurobiology and Anatomy, The Medical College of Wisconsin, Milwaukee, WI USA | ENA first public:2022 01 26|ENA last update:2022 01 26|External Id:SAMEA7984961|INSDC center alias:Department of Pediatrics and Cell Biology Neurobiology and Anatomy The Medical College of Wisconsin Milwaukee WI USA|INSDC center name:Department of Pediatrics and Cell Biology Neurobiology and Anatomy The Medical College of Wisconsin Milwaukee WI USA|INSDC first public:2022 01 26T00:16:15Z|INSDC last update:2022 01 26T00:16:15Z|INSDC status:public|Submitter Id:E MTAB 10068:Zebrafish mab21l1 2|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:late embryonic stage|genotype:homozygous mab21l1 c.107delA|organism part:eye|sample name:E MTAB 10068:Zebrafish mab21l1 2 | Illumina NovaSeq 6000 paired end sequencing; RNA Seq of zebrafish whole eye comparing wild type and mab21l1 c.107delA p.Lys36Argfs*7 embryos at 5 dpf | E MTAB 10068:Zebrafish mab21l1 2 p | Zebrafish mab21l1 2 p | RNA Seq of zebrafish whole eye comparing wild type and mab21l1 c.107delA p.Lys36Argfs*7 embryos at 5 dpf | Whole eyes were dissected from 5 dpf wild type or homozygous mab21l1 c.107delA embryos into 1X Ringer's solution. Three biological samples were collected per genotype and each sample contained 50 eyes. To isolate RNA whole eye samples were lysed and homogenized in TRI Reagent. Then the Direct zol RNA MiniPrep Kit from ZymoResearch was followed to extract and purify RNA. RNA Samples were submitted to Macrogen for RNA Seq performance. Macrogen conducted library prep using a TruSeq Stranded mRNA LT Sample Prep Kit following the TruSeq Stranded mRNA Sample Preparation Guide Part # 15031047 Rev. E. | Experimental Factor: genotype:homozygous mab21l1 c.107delA | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | ERP126773 | Illumina NovaSeq 6000 paired end sequencing; RNA Seq of zebrafish whole eye comparing wild type and mab21l1 c.107delA p.Lys36Argfs*7 embryos at 5 dpf | ENA FIRST PUBLIC:2022 01 26|ENA LAST UPDATE:2022 01 26 | Zebrafish_mab21l1_2_1.fastq.gz Zebrafish_mab21l1_2_2.fastq.gz | fastq fastq | 6989382602.0 | 23143651.0 | E MTAB 10068:Zebrafish mab21l1 2 | 0:151 1:151 | A:1841093814;C:1660652130;G:1754262273;T:1733319973;N:54412 | 151 | 151 | 1841093814 | 1660652130 | 1754262273 | 1733319973 | 54412 | ERX5039559 | ERS5672243 | ERA3400155 | Department of Pediatrics and Cell Biology, Neurobiology and Anatomy, The Medical College of Wisconsin, Milwaukee, WI USA|European Nucleotide Archive | Department of Pediatrics and Cell Biology, Neurobiology and Anatomy, The Medical College of Wisconsin, Milwaukee, WI USA|European Nucleotide Archive | 2 | 0.95595 | 0.95712 | 0.07199 | 0.07107 | 0.70485 | 0.70431 | 0.42033 | 0.42681 | 151 | 151 | B | B | biological fallback assumption | illumina | novaseq_era | unknown | poly_a | trueseq | bulk | unknown | unknown | United States | 2022-01-26 | Larval | Larval | Eye | Sensory System | |||||||||||||
| 10160 | 10160 | ERR5236191 | ERX5039558 | ERS5672242 | ERP126773 | PRJEB42852 | RNA Seq of zebrafish whole eye comparing wild type and mab21l1 c.107delA p.Lys36Argfs*7 embryos at 5 dpf | E-MTAB-10068 | Transcriptome Analysis | Currently there is little knowledge on the functions and signaling pathways of MAB21L1. Therefore we developed a zebrafish genetic model of mab21l1 deficiency to use in order to gain insight into possibly affected pathways and transcripts. We conducted RNA seq on zebrafish whole eyes which were dissected out at 5 dpf Three biological replicates per genotype wild type or homozygous mab21l1 c.107delA were collected with 50 eyes per replicate. RNA was extracted and purified and submitted to Macrogen to conduct RNA Sequencing procedure. | ENA FIRST PUBLIC:2022 01 26|ENA LAST UPDATE:2022 01 26 | Protocols: Whole eyes were dissected from 5 dpf wild type or homozygous mab21l1 c.107delA embryos into 1X Ringer's solution. Three biological samples were collected per genotype and each sample contained 50 eyes. To isolate RNA whole eye samples were lysed and homogenized in TRI Reagent. Then the Direct zol RNA MiniPrep Kit from ZymoResearch was followed to extract and purify RNA. RNA Samples were submitted to Macrogen for RNA Seq performance. Macrogen conducted library prep using a TruSeq Stranded mRNA LT Sample Prep Kit following the TruSeq Stranded mRNA Sample Preparation Guide Part # 15031047 Rev. E. | Zebrafish mab21l1 1 | SAMEA7984960 | Department of Pediatrics and Cell Biology, Neurobiology and Anatomy, The Medical College of Wisconsin, Milwaukee, WI USA | ENA first public:2022 01 26|ENA last update:2022 01 26|External Id:SAMEA7984960|INSDC center alias:Department of Pediatrics and Cell Biology Neurobiology and Anatomy The Medical College of Wisconsin Milwaukee WI USA|INSDC center name:Department of Pediatrics and Cell Biology Neurobiology and Anatomy The Medical College of Wisconsin Milwaukee WI USA|INSDC first public:2022 01 26T00:16:15Z|INSDC last update:2022 01 26T00:16:15Z|INSDC status:public|Submitter Id:E MTAB 10068:Zebrafish mab21l1 1|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:late embryonic stage|genotype:homozygous mab21l1 c.107delA|organism part:eye|sample name:E MTAB 10068:Zebrafish mab21l1 1 | Illumina NovaSeq 6000 paired end sequencing; RNA Seq of zebrafish whole eye comparing wild type and mab21l1 c.107delA p.Lys36Argfs*7 embryos at 5 dpf | E MTAB 10068:Zebrafish mab21l1 1 p | Zebrafish mab21l1 1 p | RNA Seq of zebrafish whole eye comparing wild type and mab21l1 c.107delA p.Lys36Argfs*7 embryos at 5 dpf | Whole eyes were dissected from 5 dpf wild type or homozygous mab21l1 c.107delA embryos into 1X Ringer's solution. Three biological samples were collected per genotype and each sample contained 50 eyes. To isolate RNA whole eye samples were lysed and homogenized in TRI Reagent. Then the Direct zol RNA MiniPrep Kit from ZymoResearch was followed to extract and purify RNA. RNA Samples were submitted to Macrogen for RNA Seq performance. Macrogen conducted library prep using a TruSeq Stranded mRNA LT Sample Prep Kit following the TruSeq Stranded mRNA Sample Preparation Guide Part # 15031047 Rev. E. | Experimental Factor: genotype:homozygous mab21l1 c.107delA | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | ERP126773 | Illumina NovaSeq 6000 paired end sequencing; RNA Seq of zebrafish whole eye comparing wild type and mab21l1 c.107delA p.Lys36Argfs*7 embryos at 5 dpf | ENA FIRST PUBLIC:2022 01 26|ENA LAST UPDATE:2022 01 26 | Zebrafish_mab21l1_1_1.fastq.gz Zebrafish_mab21l1_1_2.fastq.gz | fastq fastq | 6882371922.0 | 22789311.0 | E MTAB 10068:Zebrafish mab21l1 1 | 0:151 1:151 | A:1803380739;C:1630414039;G:1732263332;T:1716255495;N:58317 | 151 | 151 | 1803380739 | 1630414039 | 1732263332 | 1716255495 | 58317 | ERX5039558 | ERS5672242 | ERA3400155 | Department of Pediatrics and Cell Biology, Neurobiology and Anatomy, The Medical College of Wisconsin, Milwaukee, WI USA|European Nucleotide Archive | Department of Pediatrics and Cell Biology, Neurobiology and Anatomy, The Medical College of Wisconsin, Milwaukee, WI USA|European Nucleotide Archive | 2 | 0.95518 | 0.95612 | 0.07088 | 0.07032 | 0.70526 | 0.70457 | 0.42942 | 0.4302 | 151 | 151 | B | B | biological fallback assumption | illumina | novaseq_era | unknown | poly_a | trueseq | bulk | unknown | unknown | United States | 2022-01-26 | Larval | Larval | Eye | Sensory System | |||||||||||||
| 30230 | 30230 | SRR27730907 | SRX23396554 | SRS20257429 | SRP486007 | PRJNA1069290 | Noninvasive light flicker stimulation promotes optic nerve regeneration by activating microglia and enhancing neural plasticity in zebrafish | PRJNA1069290 | Other | In the present study we demonstrated that the optic nerve can spontaneously regenerate and that visual function can be restored post ONC in zebrafish. Forty Hertz light flicker stimulation modulated microglia in the zebrafish retina post ONC promoted dendritic remodeling and axonal regeneration of RGCs and restored visual function. Transcriptomic and qPCR results both indicated that 40 Hz light flicker stimulation increased the expression of genes related to neural plasticity. In general our study showed that 40 Hz light flicker stimulation promotes axonal and dendritic regeneration of RGCs as well as the recovery of visual function which is related to the activation of microglia and enhancement of neural plasticity. | 4dpi40Hz+biological replicate 2 | 4dpi40Hz 2 | strain:AB strain|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|age:3 month 6 month|dev stage:3 month 6 month|collection date:2023 07|geo loc name:China:Nanchang|sex:pooled male and female|tissue:retina|ID:10|BioSampleModel:Model organism or animal | RNA seq of Danio rerio: retina | M23165700aR1AL1A | M23165700aR1AL1A | 4 xxx post injury+40Hz | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP486007 | 4dpi40Hz_2_1.fq.gz 4dpi40Hz_2_2.fq.gz | fastq fastq | 7794055500.0 | 25980185.0 | 4dpi40Hz 2 1.fq.gz | 0:150 1:150 | A:2129184357;C:1759642251;G:1791618685;T:2113568820;N:41387 | 150 | 150 | 2129184357 | 1759642251 | 1791618685 | 2113568820 | 41387 | SRX23396554 | SRS20257429 | SRA1791874 | Affiliated Eye Hospital of Nanchang University|Department of Ophthalmology | Affiliated Eye Hospital of Nanchang University | B | B | biological fallback assumption | illumina | novaseq_era | unknown | poly_a | unknown | bulk | unknown | unknown | China | 2024-01-25 | Adult | Adult | Eye | Sensory System | |||||||||||||||||||||||||||||||
| 30231 | 30231 | SRR27730908 | SRX23396553 | SRS20257428 | SRP486007 | PRJNA1069290 | Noninvasive light flicker stimulation promotes optic nerve regeneration by activating microglia and enhancing neural plasticity in zebrafish | PRJNA1069290 | Other | In the present study we demonstrated that the optic nerve can spontaneously regenerate and that visual function can be restored post ONC in zebrafish. Forty Hertz light flicker stimulation modulated microglia in the zebrafish retina post ONC promoted dendritic remodeling and axonal regeneration of RGCs and restored visual function. Transcriptomic and qPCR results both indicated that 40 Hz light flicker stimulation increased the expression of genes related to neural plasticity. In general our study showed that 40 Hz light flicker stimulation promotes axonal and dendritic regeneration of RGCs as well as the recovery of visual function which is related to the activation of microglia and enhancement of neural plasticity. | 4dpi40Hz+biological replicate 1 | 4dpi40Hz 1 | strain:AB strain|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|age:3 month 6 month|dev stage:3 month 6 month|collection date:2023 07|geo loc name:China:Nanchang|sex:pooled male and female|tissue:retina|ID:9|BioSampleModel:Model organism or animal | RNA seq of Danio rerio: retina | M23165699aR1AL1A | M23165699aR1AL1A | 4 xxx post injury+40Hz | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP486007 | 4dpi40Hz_1_1.fq.gz 4dpi40Hz_1_2.fq.gz | fastq fastq | 7508652600.0 | 25028842.0 | 4dpi40Hz 1 1.fq.gz | 0:150 1:150 | A:2057198043;C:1689714910;G:1722042670;T:2039656491;N:40486 | 150 | 150 | 2057198043 | 1689714910 | 1722042670 | 2039656491 | 40486 | SRX23396553 | SRS20257428 | SRA1791874 | Affiliated Eye Hospital of Nanchang University|Department of Ophthalmology | Affiliated Eye Hospital of Nanchang University | B | B | biological fallback assumption | illumina | novaseq_era | unknown | poly_a | unknown | bulk | unknown | unknown | China | 2024-01-25 | Adult | Adult | Eye | Sensory System | |||||||||||||||||||||||||||||||
| 30232 | 30232 | SRR27730909 | SRX23396552 | SRS20257427 | SRP486007 | PRJNA1069290 | Noninvasive light flicker stimulation promotes optic nerve regeneration by activating microglia and enhancing neural plasticity in zebrafish | PRJNA1069290 | Other | In the present study we demonstrated that the optic nerve can spontaneously regenerate and that visual function can be restored post ONC in zebrafish. Forty Hertz light flicker stimulation modulated microglia in the zebrafish retina post ONC promoted dendritic remodeling and axonal regeneration of RGCs and restored visual function. Transcriptomic and qPCR results both indicated that 40 Hz light flicker stimulation increased the expression of genes related to neural plasticity. In general our study showed that 40 Hz light flicker stimulation promotes axonal and dendritic regeneration of RGCs as well as the recovery of visual function which is related to the activation of microglia and enhancement of neural plasticity. | 4dpi+biological replicate 4 | 4dpi 4 | strain:AB strain|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|age:3 month 6 month|dev stage:3 month 6 month|collection date:2023 07|geo loc name:China:Nanchang|sex:pooled male and female|tissue:retina|ID:8|BioSampleModel:Model organism or animal | RNA seq of Danio rerio: retina | M23165697aR1AL1A | M23165697aR1AL1A | 4 xxx post injury | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP486007 | 4dpi_4_1.fq.gz 4dpi_4_2.fq.gz | fastq fastq | 7094977200.0 | 23649924.0 | 4dpi 4 1.fq.gz | 0:150 1:150 | A:1943019606;C:1597025881;G:1626301543;T:1928592039;N:38131 | 150 | 150 | 1943019606 | 1597025881 | 1626301543 | 1928592039 | 38131 | SRX23396552 | SRS20257427 | SRA1791874 | Affiliated Eye Hospital of Nanchang University|Department of Ophthalmology | Affiliated Eye Hospital of Nanchang University | B | B | biological fallback assumption | illumina | novaseq_era | unknown | poly_a | unknown | bulk | unknown | unknown | China | 2024-01-25 | Adult | Adult | Eye | Sensory System | |||||||||||||||||||||||||||||||
| 30233 | 30233 | SRR27730910 | SRX23396551 | SRS20257426 | SRP486007 | PRJNA1069290 | Noninvasive light flicker stimulation promotes optic nerve regeneration by activating microglia and enhancing neural plasticity in zebrafish | PRJNA1069290 | Other | In the present study we demonstrated that the optic nerve can spontaneously regenerate and that visual function can be restored post ONC in zebrafish. Forty Hertz light flicker stimulation modulated microglia in the zebrafish retina post ONC promoted dendritic remodeling and axonal regeneration of RGCs and restored visual function. Transcriptomic and qPCR results both indicated that 40 Hz light flicker stimulation increased the expression of genes related to neural plasticity. In general our study showed that 40 Hz light flicker stimulation promotes axonal and dendritic regeneration of RGCs as well as the recovery of visual function which is related to the activation of microglia and enhancement of neural plasticity. | 4dpi+biological replicate 3 | 4dpi 3 | strain:AB strain|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|age:3 month 6 month|dev stage:3 month 6 month|collection date:2023 07|geo loc name:China:Nanchang|sex:pooled male and female|tissue:retina|ID:7|BioSampleModel:Model organism or animal | RNA seq of Danio rerio: retina | M23165696aR1AL1A | M23165696aR1AL1A | 4 xxx post injury | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP486007 | 4dpi_3_1.fq.gz 4dpi_3_2.fq.gz | fastq fastq | 7386629700.0 | 24622099.0 | 4dpi 3 1.fq.gz | 0:150 1:150 | A:2031801635;C:1655982922;G:1683647412;T:2015157737;N:39994 | 150 | 150 | 2031801635 | 1655982922 | 1683647412 | 2015157737 | 39994 | SRX23396551 | SRS20257426 | SRA1791874 | Affiliated Eye Hospital of Nanchang University|Department of Ophthalmology | Affiliated Eye Hospital of Nanchang University | B | B | biological fallback assumption | illumina | novaseq_era | unknown | poly_a | unknown | bulk | unknown | unknown | China | 2024-01-25 | Adult | Adult | Eye | Sensory System | |||||||||||||||||||||||||||||||
| 30234 | 30234 | SRR27730911 | SRX23396550 | SRS20257425 | SRP486007 | PRJNA1069290 | Noninvasive light flicker stimulation promotes optic nerve regeneration by activating microglia and enhancing neural plasticity in zebrafish | PRJNA1069290 | Other | In the present study we demonstrated that the optic nerve can spontaneously regenerate and that visual function can be restored post ONC in zebrafish. Forty Hertz light flicker stimulation modulated microglia in the zebrafish retina post ONC promoted dendritic remodeling and axonal regeneration of RGCs and restored visual function. Transcriptomic and qPCR results both indicated that 40 Hz light flicker stimulation increased the expression of genes related to neural plasticity. In general our study showed that 40 Hz light flicker stimulation promotes axonal and dendritic regeneration of RGCs as well as the recovery of visual function which is related to the activation of microglia and enhancement of neural plasticity. | 4dpi+biological replicate 2 | 4dpi 2 | strain:AB strain|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|age:3 month 6 month|dev stage:3 month 6 month|collection date:2023 07|geo loc name:China:Nanchang|sex:pooled male and female|tissue:retina|ID:6|BioSampleModel:Model organism or animal | RNA seq of Danio rerio: retina | M23165695aR1AL1A | M23165695aR1AL1A | 4 xxx post injury | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP486007 | 4dpi_2_1.fq.gz 4dpi_2_2.fq.gz | fastq fastq | 6654237600.0 | 22180792.0 | 4dpi 2 1.fq.gz | 0:150 1:150 | A:1825547718;C:1496727215;G:1522459901;T:1809467151;N:35615 | 150 | 150 | 1825547718 | 1496727215 | 1522459901 | 1809467151 | 35615 | SRX23396550 | SRS20257425 | SRA1791874 | Affiliated Eye Hospital of Nanchang University|Department of Ophthalmology | Affiliated Eye Hospital of Nanchang University | B | B | biological fallback assumption | illumina | novaseq_era | unknown | poly_a | unknown | bulk | unknown | unknown | China | 2024-01-25 | Adult | Adult | Eye | Sensory System | |||||||||||||||||||||||||||||||
| 30235 | 30235 | SRR27730912 | SRX23396549 | SRS20257424 | SRP486007 | PRJNA1069290 | Noninvasive light flicker stimulation promotes optic nerve regeneration by activating microglia and enhancing neural plasticity in zebrafish | PRJNA1069290 | Other | In the present study we demonstrated that the optic nerve can spontaneously regenerate and that visual function can be restored post ONC in zebrafish. Forty Hertz light flicker stimulation modulated microglia in the zebrafish retina post ONC promoted dendritic remodeling and axonal regeneration of RGCs and restored visual function. Transcriptomic and qPCR results both indicated that 40 Hz light flicker stimulation increased the expression of genes related to neural plasticity. In general our study showed that 40 Hz light flicker stimulation promotes axonal and dendritic regeneration of RGCs as well as the recovery of visual function which is related to the activation of microglia and enhancement of neural plasticity. | 4dpi+biological replicate 1 | 4dpi 1 | strain:AB strain|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|age:3 month 6 month|dev stage:3 month 6 month|collection date:2023 07|geo loc name:China:Nanchang|sex:pooled male and female|tissue:retina|ID:5|BioSampleModel:Model organism or animal | RNA seq of Danio rerio: retina | M23165694aR1AL1A | M23165694aR1AL1A | 4 xxx post injury | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP486007 | 4dpi_1_1.fq.gz 4dpi_1_2.fq.gz | fastq fastq | 7023034200.0 | 23410114.0 | 4dpi 1 1.fq.gz | 0:150 1:150 | A:1928523555;C:1581982138;G:1606070304;T:1906420119;N:38084 | 150 | 150 | 1928523555 | 1581982138 | 1606070304 | 1906420119 | 38084 | SRX23396549 | SRS20257424 | SRA1791874 | Affiliated Eye Hospital of Nanchang University|Department of Ophthalmology | Affiliated Eye Hospital of Nanchang University | B | B | biological fallback assumption | illumina | novaseq_era | unknown | poly_a | unknown | bulk | unknown | unknown | China | 2024-01-25 | Adult | Adult | Eye | Sensory System | |||||||||||||||||||||||||||||||
| 30236 | 30236 | SRR27730913 | SRX23396548 | SRS20257423 | SRP486007 | PRJNA1069290 | Noninvasive light flicker stimulation promotes optic nerve regeneration by activating microglia and enhancing neural plasticity in zebrafish | PRJNA1069290 | Other | In the present study we demonstrated that the optic nerve can spontaneously regenerate and that visual function can be restored post ONC in zebrafish. Forty Hertz light flicker stimulation modulated microglia in the zebrafish retina post ONC promoted dendritic remodeling and axonal regeneration of RGCs and restored visual function. Transcriptomic and qPCR results both indicated that 40 Hz light flicker stimulation increased the expression of genes related to neural plasticity. In general our study showed that 40 Hz light flicker stimulation promotes axonal and dendritic regeneration of RGCs as well as the recovery of visual function which is related to the activation of microglia and enhancement of neural plasticity. | Con+biological replicate 4 | Con 4 | strain:AB strain|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|age:3 month 6 month|dev stage:3 month 6 month|collection date:2023 07|geo loc name:China:Nanchang|sex:pooled male and female|tissue:retina|ID:4|BioSampleModel:Model organism or animal | RNA seq of Danio rerio: retina | M23165693aR1AL1A | M23165693aR1AL1A | Control | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP486007 | Con_4_1.fq.gz Con_4_2.fq.gz | fastq fastq | 6857548800.0 | 22858496.0 | Con 4 1.fq.gz | 0:150 1:150 | A:1882601360;C:1546021101;G:1569334195;T:1859555495;N:36649 | 150 | 150 | 1882601360 | 1546021101 | 1569334195 | 1859555495 | 36649 | SRX23396548 | SRS20257423 | SRA1791874 | Affiliated Eye Hospital of Nanchang University|Department of Ophthalmology | Affiliated Eye Hospital of Nanchang University | B | B | biological fallback assumption | illumina | novaseq_era | unknown | poly_a | unknown | bulk | unknown | unknown | China | 2024-01-25 | Adult | Adult | Eye | Sensory System | |||||||||||||||||||||||||||||||
| 30237 | 30237 | SRR27730914 | SRX23396547 | SRS20257422 | SRP486007 | PRJNA1069290 | Noninvasive light flicker stimulation promotes optic nerve regeneration by activating microglia and enhancing neural plasticity in zebrafish | PRJNA1069290 | Other | In the present study we demonstrated that the optic nerve can spontaneously regenerate and that visual function can be restored post ONC in zebrafish. Forty Hertz light flicker stimulation modulated microglia in the zebrafish retina post ONC promoted dendritic remodeling and axonal regeneration of RGCs and restored visual function. Transcriptomic and qPCR results both indicated that 40 Hz light flicker stimulation increased the expression of genes related to neural plasticity. In general our study showed that 40 Hz light flicker stimulation promotes axonal and dendritic regeneration of RGCs as well as the recovery of visual function which is related to the activation of microglia and enhancement of neural plasticity. | Con+biological replicate 3 | Con 3 | strain:AB strain|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|age:3 month 6 month|dev stage:3 month 6 month|collection date:2023 07|geo loc name:China:Nanchang|sex:pooled male and female|tissue:retina|ID:3|BioSampleModel:Model organism or animal | RNA seq of Danio rerio: retina | M23165692aR1AL1A | M23165692aR1AL1A | Control | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP486007 | Con_3_1.fq.gz Con_3_2.fq.gz | fastq fastq | 7458957900.0 | 24863193.0 | Con 3 1.fq.gz | 0:150 1:150 | A:2038911157;C:1679971639;G:1712675120;T:2027359083;N:40901 | 150 | 150 | 2038911157 | 1679971639 | 1712675120 | 2027359083 | 40901 | SRX23396547 | SRS20257422 | SRA1791874 | Affiliated Eye Hospital of Nanchang University|Department of Ophthalmology | Affiliated Eye Hospital of Nanchang University | B | B | biological fallback assumption | illumina | novaseq_era | unknown | poly_a | unknown | bulk | unknown | unknown | China | 2024-01-25 | Adult | Adult | Eye | Sensory System | |||||||||||||||||||||||||||||||
| 30238 | 30238 | SRR27730915 | SRX23396546 | SRS20257421 | SRP486007 | PRJNA1069290 | Noninvasive light flicker stimulation promotes optic nerve regeneration by activating microglia and enhancing neural plasticity in zebrafish | PRJNA1069290 | Other | In the present study we demonstrated that the optic nerve can spontaneously regenerate and that visual function can be restored post ONC in zebrafish. Forty Hertz light flicker stimulation modulated microglia in the zebrafish retina post ONC promoted dendritic remodeling and axonal regeneration of RGCs and restored visual function. Transcriptomic and qPCR results both indicated that 40 Hz light flicker stimulation increased the expression of genes related to neural plasticity. In general our study showed that 40 Hz light flicker stimulation promotes axonal and dendritic regeneration of RGCs as well as the recovery of visual function which is related to the activation of microglia and enhancement of neural plasticity. | 7dpi40Hz+biological replicate 4 | 7dpi40Hz 4 | strain:AB strain|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|age:3 month 6 month|dev stage:3 month 6 month|collection date:2023 07|geo loc name:China:Nanchang|sex:pooled male and female|tissue:retina|ID:20|BioSampleModel:Model organism or animal | RNA seq of Danio rerio: retina | M23165713aR1AL1A | M23165713aR1AL1A | 7 xxx post injury+40Hz | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP486007 | 7dpi40Hz_4_1.fq.gz 7dpi40Hz_4_2.fq.gz | fastq fastq | 6691700400.0 | 22305668.0 | 7dpi40Hz 4 1.fq.gz | 0:150 1:150 | A:1868931421;C:1476540468;G:1501092489;T:1845097964;N:38058 | 150 | 150 | 1868931421 | 1476540468 | 1501092489 | 1845097964 | 38058 | SRX23396546 | SRS20257421 | SRA1791874 | Affiliated Eye Hospital of Nanchang University|Department of Ophthalmology | Affiliated Eye Hospital of Nanchang University | B | B | biological fallback assumption | illumina | novaseq_era | unknown | poly_a | unknown | bulk | unknown | unknown | China | 2024-01-25 | Adult | Adult | Eye | Sensory System | |||||||||||||||||||||||||||||||
| 30239 | 30239 | SRR27730916 | SRX23396545 | SRS20257420 | SRP486007 | PRJNA1069290 | Noninvasive light flicker stimulation promotes optic nerve regeneration by activating microglia and enhancing neural plasticity in zebrafish | PRJNA1069290 | Other | In the present study we demonstrated that the optic nerve can spontaneously regenerate and that visual function can be restored post ONC in zebrafish. Forty Hertz light flicker stimulation modulated microglia in the zebrafish retina post ONC promoted dendritic remodeling and axonal regeneration of RGCs and restored visual function. Transcriptomic and qPCR results both indicated that 40 Hz light flicker stimulation increased the expression of genes related to neural plasticity. In general our study showed that 40 Hz light flicker stimulation promotes axonal and dendritic regeneration of RGCs as well as the recovery of visual function which is related to the activation of microglia and enhancement of neural plasticity. | 7dpi40Hz+biological replicate 3 | 7dpi40Hz 3 | strain:AB strain|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|age:3 month 6 month|dev stage:3 month 6 month|collection date:2023 07|geo loc name:China:Nanchang|sex:pooled male and female|tissue:retina|ID:19|BioSampleModel:Model organism or animal | RNA seq of Danio rerio: retina | M23165712aR1AL1A | M23165712aR1AL1A | 7 xxx post injury+40Hz | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP486007 | 7dpi40Hz_3_1.fq.gz 7dpi40Hz_3_2.fq.gz | fastq fastq | 7212117000.0 | 24040390.0 | 7dpi40Hz 3 1.fq.gz | 0:150 1:150 | A:1992256057;C:1607062903;G:1637554739;T:1975202488;N:40813 | 150 | 150 | 1992256057 | 1607062903 | 1637554739 | 1975202488 | 40813 | SRX23396545 | SRS20257420 | SRA1791874 | Affiliated Eye Hospital of Nanchang University|Department of Ophthalmology | Affiliated Eye Hospital of Nanchang University | B | B | biological fallback assumption | illumina | novaseq_era | unknown | poly_a | unknown | bulk | unknown | unknown | China | 2024-01-25 | Adult | Adult | Eye | Sensory System | |||||||||||||||||||||||||||||||
| 30240 | 30240 | SRR27730917 | SRX23396544 | SRS20257419 | SRP486007 | PRJNA1069290 | Noninvasive light flicker stimulation promotes optic nerve regeneration by activating microglia and enhancing neural plasticity in zebrafish | PRJNA1069290 | Other | In the present study we demonstrated that the optic nerve can spontaneously regenerate and that visual function can be restored post ONC in zebrafish. Forty Hertz light flicker stimulation modulated microglia in the zebrafish retina post ONC promoted dendritic remodeling and axonal regeneration of RGCs and restored visual function. Transcriptomic and qPCR results both indicated that 40 Hz light flicker stimulation increased the expression of genes related to neural plasticity. In general our study showed that 40 Hz light flicker stimulation promotes axonal and dendritic regeneration of RGCs as well as the recovery of visual function which is related to the activation of microglia and enhancement of neural plasticity. | 7dpi40Hz+biological replicate 2 | 7dpi40Hz 2 | strain:AB strain|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|age:3 month 6 month|dev stage:3 month 6 month|collection date:2023 07|geo loc name:China:Nanchang|sex:pooled male and female|tissue:retina|ID:18|BioSampleModel:Model organism or animal | RNA seq of Danio rerio: retina | M23165711aR1AL1A | M23165711aR1AL1A | 7 xxx post injury+40Hz | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP486007 | 7dpi40Hz_2_1.fq.gz 7dpi40Hz_2_2.fq.gz | fastq fastq | 7457525100.0 | 24858417.0 | 7dpi40Hz 2 1.fq.gz | 0:150 1:150 | A:2040191437;C:1678135313;G:1713456206;T:2025700956;N:41188 | 150 | 150 | 2040191437 | 1678135313 | 1713456206 | 2025700956 | 41188 | SRX23396544 | SRS20257419 | SRA1791874 | Affiliated Eye Hospital of Nanchang University|Department of Ophthalmology | Affiliated Eye Hospital of Nanchang University | B | B | biological fallback assumption | illumina | novaseq_era | unknown | poly_a | unknown | bulk | unknown | unknown | China | 2024-01-25 | Adult | Adult | Eye | Sensory System | |||||||||||||||||||||||||||||||
| 30241 | 30241 | SRR27730918 | SRX23396543 | SRS20257418 | SRP486007 | PRJNA1069290 | Noninvasive light flicker stimulation promotes optic nerve regeneration by activating microglia and enhancing neural plasticity in zebrafish | PRJNA1069290 | Other | In the present study we demonstrated that the optic nerve can spontaneously regenerate and that visual function can be restored post ONC in zebrafish. Forty Hertz light flicker stimulation modulated microglia in the zebrafish retina post ONC promoted dendritic remodeling and axonal regeneration of RGCs and restored visual function. Transcriptomic and qPCR results both indicated that 40 Hz light flicker stimulation increased the expression of genes related to neural plasticity. In general our study showed that 40 Hz light flicker stimulation promotes axonal and dendritic regeneration of RGCs as well as the recovery of visual function which is related to the activation of microglia and enhancement of neural plasticity. | 7dpi40Hz+biological replicate 1 | 7dpi40Hz 1 | strain:AB strain|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|age:3 month 6 month|dev stage:3 month 6 month|collection date:2023 07|geo loc name:China:Nanchang|sex:pooled male and female|tissue:retina|ID:17|BioSampleModel:Model organism or animal | RNA seq of Danio rerio: retina | M23165710aR1AL1A | M23165710aR1AL1A | 7 xxx post injury+40Hz | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP486007 | 7dpi40Hz_1_1.fq.gz 7dpi40Hz_1_2.fq.gz | fastq fastq | 6855385800.0 | 22851286.0 | 7dpi40Hz 1 1.fq.gz | 0:150 1:150 | A:1880374717;C:1545013977;G:1569663640;T:1860295686;N:37780 | 150 | 150 | 1880374717 | 1545013977 | 1569663640 | 1860295686 | 37780 | SRX23396543 | SRS20257418 | SRA1791874 | Affiliated Eye Hospital of Nanchang University|Department of Ophthalmology | Affiliated Eye Hospital of Nanchang University | B | B | biological fallback assumption | illumina | novaseq_era | unknown | poly_a | unknown | bulk | unknown | unknown | China | 2024-01-25 | Adult | Adult | Eye | Sensory System | |||||||||||||||||||||||||||||||
| 30242 | 30242 | SRR27730919 | SRX23396542 | SRS20257417 | SRP486007 | PRJNA1069290 | Noninvasive light flicker stimulation promotes optic nerve regeneration by activating microglia and enhancing neural plasticity in zebrafish | PRJNA1069290 | Other | In the present study we demonstrated that the optic nerve can spontaneously regenerate and that visual function can be restored post ONC in zebrafish. Forty Hertz light flicker stimulation modulated microglia in the zebrafish retina post ONC promoted dendritic remodeling and axonal regeneration of RGCs and restored visual function. Transcriptomic and qPCR results both indicated that 40 Hz light flicker stimulation increased the expression of genes related to neural plasticity. In general our study showed that 40 Hz light flicker stimulation promotes axonal and dendritic regeneration of RGCs as well as the recovery of visual function which is related to the activation of microglia and enhancement of neural plasticity. | 7dpi+biological replicate 4 | 7dpi 4 | strain:AB strain|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|age:3 month 6 month|dev stage:3 month 6 month|collection date:2023 07|geo loc name:China:Nanchang|sex:pooled male and female|tissue:retina|ID:16|BioSampleModel:Model organism or animal | RNA seq of Danio rerio: retina | M23165708aR1AL1A | M23165708aR1AL1A | 7 xxx post injury | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP486007 | 7dpi_4_1.fq.gz 7dpi_4_2.fq.gz | fastq fastq | 6462470400.0 | 21541568.0 | 7dpi 4 1.fq.gz | 0:150 1:150 | A:1785795783;C:1441222995;G:1466593271;T:1768826748;N:31603 | 150 | 150 | 1785795783 | 1441222995 | 1466593271 | 1768826748 | 31603 | SRX23396542 | SRS20257417 | SRA1791874 | Affiliated Eye Hospital of Nanchang University|Department of Ophthalmology | Affiliated Eye Hospital of Nanchang University | B | B | biological fallback assumption | illumina | novaseq_era | unknown | poly_a | unknown | bulk | unknown | unknown | China | 2024-01-25 | Adult | Adult | Eye | Sensory System | |||||||||||||||||||||||||||||||
| 30243 | 30243 | SRR27730920 | SRX23396541 | SRS20257416 | SRP486007 | PRJNA1069290 | Noninvasive light flicker stimulation promotes optic nerve regeneration by activating microglia and enhancing neural plasticity in zebrafish | PRJNA1069290 | Other | In the present study we demonstrated that the optic nerve can spontaneously regenerate and that visual function can be restored post ONC in zebrafish. Forty Hertz light flicker stimulation modulated microglia in the zebrafish retina post ONC promoted dendritic remodeling and axonal regeneration of RGCs and restored visual function. Transcriptomic and qPCR results both indicated that 40 Hz light flicker stimulation increased the expression of genes related to neural plasticity. In general our study showed that 40 Hz light flicker stimulation promotes axonal and dendritic regeneration of RGCs as well as the recovery of visual function which is related to the activation of microglia and enhancement of neural plasticity. | 7dpi+biological replicate 3 | 7dpi 3 | strain:AB strain|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|age:3 month 6 month|dev stage:3 month 6 month|collection date:2023 07|geo loc name:China:Nanchang|sex:pooled male and female|tissue:retina|ID:15|BioSampleModel:Model organism or animal | RNA seq of Danio rerio: retina | M23165706aR1AL1A | M23165706aR1AL1A | 7 xxx post injury | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP486007 | 7dpi_3_1.fq.gz 7dpi_3_2.fq.gz | fastq fastq | 6710491200.0 | 22368304.0 | 7dpi 3 1.fq.gz | 0:150 1:150 | A:1831876501;C:1512529252;G:1544827061;T:1821200080;N:58306 | 150 | 150 | 1831876501 | 1512529252 | 1544827061 | 1821200080 | 58306 | SRX23396541 | SRS20257416 | SRA1791874 | Affiliated Eye Hospital of Nanchang University|Department of Ophthalmology | Affiliated Eye Hospital of Nanchang University | B | B | biological fallback assumption | illumina | novaseq_era | unknown | poly_a | unknown | bulk | unknown | unknown | China | 2024-01-25 | Adult | Adult | Eye | Sensory System | |||||||||||||||||||||||||||||||
| 30244 | 30244 | SRR27730921 | SRX23396540 | SRS20257415 | SRP486007 | PRJNA1069290 | Noninvasive light flicker stimulation promotes optic nerve regeneration by activating microglia and enhancing neural plasticity in zebrafish | PRJNA1069290 | Other | In the present study we demonstrated that the optic nerve can spontaneously regenerate and that visual function can be restored post ONC in zebrafish. Forty Hertz light flicker stimulation modulated microglia in the zebrafish retina post ONC promoted dendritic remodeling and axonal regeneration of RGCs and restored visual function. Transcriptomic and qPCR results both indicated that 40 Hz light flicker stimulation increased the expression of genes related to neural plasticity. In general our study showed that 40 Hz light flicker stimulation promotes axonal and dendritic regeneration of RGCs as well as the recovery of visual function which is related to the activation of microglia and enhancement of neural plasticity. | 7dpi+biological replicate 2 | 7dpi 2 | strain:AB strain|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|age:3 month 6 month|dev stage:3 month 6 month|collection date:2023 07|geo loc name:China:Nanchang|sex:pooled male and female|tissue:retina|ID:14|BioSampleModel:Model organism or animal | RNA seq of Danio rerio: retina | M23165705aR1AL1A | M23165705aR1AL1A | 7 xxx post injury | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP486007 | 7dpi_2_1.fq.gz 7dpi_2_2.fq.gz | fastq fastq | 6294917700.0 | 20983059.0 | 7dpi 2 1.fq.gz | 0:150 1:150 | A:1735431442;C:1394112982;G:1431966224;T:1733339379;N:67673 | 150 | 150 | 1735431442 | 1394112982 | 1431966224 | 1733339379 | 67673 | SRX23396540 | SRS20257415 | SRA1791874 | Affiliated Eye Hospital of Nanchang University|Department of Ophthalmology | Affiliated Eye Hospital of Nanchang University | B | B | biological fallback assumption | illumina | novaseq_era | unknown | poly_a | unknown | bulk | unknown | unknown | China | 2024-01-25 | Adult | Adult | Eye | Sensory System | |||||||||||||||||||||||||||||||
| 30245 | 30245 | SRR27730922 | SRX23396539 | SRS20257414 | SRP486007 | PRJNA1069290 | Noninvasive light flicker stimulation promotes optic nerve regeneration by activating microglia and enhancing neural plasticity in zebrafish | PRJNA1069290 | Other | In the present study we demonstrated that the optic nerve can spontaneously regenerate and that visual function can be restored post ONC in zebrafish. Forty Hertz light flicker stimulation modulated microglia in the zebrafish retina post ONC promoted dendritic remodeling and axonal regeneration of RGCs and restored visual function. Transcriptomic and qPCR results both indicated that 40 Hz light flicker stimulation increased the expression of genes related to neural plasticity. In general our study showed that 40 Hz light flicker stimulation promotes axonal and dendritic regeneration of RGCs as well as the recovery of visual function which is related to the activation of microglia and enhancement of neural plasticity. | 7dpi+biological replicate 1 | 7dpi 1 | strain:AB strain|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|age:3 month 6 month|dev stage:3 month 6 month|collection date:2023 07|geo loc name:China:Nanchang|sex:pooled male and female|tissue:retina|ID:13|BioSampleModel:Model organism or animal | RNA seq of Danio rerio: retina | M23165704aR1AL1A | M23165704aR1AL1A | 7 xxx post injury | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP486007 | 7dpi_1_1.fq.gz 7dpi_1_2.fq.gz | fastq fastq | 6694150800.0 | 22313836.0 | 7dpi 1 1.fq.gz | 0:150 1:150 | A:1824840998;C:1510909671;G:1543521247;T:1814842377;N:36507 | 150 | 150 | 1824840998 | 1510909671 | 1543521247 | 1814842377 | 36507 | SRX23396539 | SRS20257414 | SRA1791874 | Affiliated Eye Hospital of Nanchang University|Department of Ophthalmology | Affiliated Eye Hospital of Nanchang University | B | B | biological fallback assumption | illumina | novaseq_era | unknown | poly_a | unknown | bulk | unknown | unknown | China | 2024-01-25 | Adult | Adult | Eye | Sensory System | |||||||||||||||||||||||||||||||
| 30246 | 30246 | SRR27730923 | SRX23396538 | SRS20257413 | SRP486007 | PRJNA1069290 | Noninvasive light flicker stimulation promotes optic nerve regeneration by activating microglia and enhancing neural plasticity in zebrafish | PRJNA1069290 | Other | In the present study we demonstrated that the optic nerve can spontaneously regenerate and that visual function can be restored post ONC in zebrafish. Forty Hertz light flicker stimulation modulated microglia in the zebrafish retina post ONC promoted dendritic remodeling and axonal regeneration of RGCs and restored visual function. Transcriptomic and qPCR results both indicated that 40 Hz light flicker stimulation increased the expression of genes related to neural plasticity. In general our study showed that 40 Hz light flicker stimulation promotes axonal and dendritic regeneration of RGCs as well as the recovery of visual function which is related to the activation of microglia and enhancement of neural plasticity. | 4dpi40Hz+biological replicate 4 | 4dpi40Hz 4 | strain:AB strain|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|age:3 month 6 month|dev stage:3 month 6 month|collection date:2023 07|geo loc name:China:Nanchang|sex:pooled male and female|tissue:retina|ID:12|BioSampleModel:Model organism or animal | RNA seq of Danio rerio: retina | M23165702aR1AL1A | M23165702aR1AL1A | 4 xxx post injury+40Hz | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP486007 | 4dpi40Hz_4_1.fq.gz 4dpi40Hz_4_2.fq.gz | fastq fastq | 8672814300.0 | 28909381.0 | 4dpi40Hz 4 1.fq.gz | 0:150 1:150 | A:2372923011;C:1956760768;G:1989924842;T:2353159305;N:46374 | 150 | 150 | 2372923011 | 1956760768 | 1989924842 | 2353159305 | 46374 | SRX23396538 | SRS20257413 | SRA1791874 | Affiliated Eye Hospital of Nanchang University|Department of Ophthalmology | Affiliated Eye Hospital of Nanchang University | B | B | biological fallback assumption | illumina | novaseq_era | unknown | poly_a | unknown | bulk | unknown | unknown | China | 2024-01-25 | Adult | Adult | Eye | Sensory System | |||||||||||||||||||||||||||||||
| 30247 | 30247 | SRR27730924 | SRX23396537 | SRS20257412 | SRP486007 | PRJNA1069290 | Noninvasive light flicker stimulation promotes optic nerve regeneration by activating microglia and enhancing neural plasticity in zebrafish | PRJNA1069290 | Other | In the present study we demonstrated that the optic nerve can spontaneously regenerate and that visual function can be restored post ONC in zebrafish. Forty Hertz light flicker stimulation modulated microglia in the zebrafish retina post ONC promoted dendritic remodeling and axonal regeneration of RGCs and restored visual function. Transcriptomic and qPCR results both indicated that 40 Hz light flicker stimulation increased the expression of genes related to neural plasticity. In general our study showed that 40 Hz light flicker stimulation promotes axonal and dendritic regeneration of RGCs as well as the recovery of visual function which is related to the activation of microglia and enhancement of neural plasticity. | 4dpi40Hz+biological replicate 3 | 4dpi40Hz 3 | strain:AB strain|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|age:3 month 6 month|dev stage:3 month 6 month|collection date:2023 07|geo loc name:China:Nanchang|sex:pooled male and female|tissue:retina|ID:11|BioSampleModel:Model organism or animal | RNA seq of Danio rerio: retina | M23165701aR1AL1A | M23165701aR1AL1A | 4 xxx post injury+40Hz | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP486007 | 4dpi40Hz_3_1.fq.gz 4dpi40Hz_3_2.fq.gz | fastq fastq | 7566042300.0 | 25220141.0 | 4dpi40Hz 3 1.fq.gz | 0:150 1:150 | A:2073697374;C:1705150021;G:1733548148;T:2053605856;N:40901 | 150 | 150 | 2073697374 | 1705150021 | 1733548148 | 2053605856 | 40901 | SRX23396537 | SRS20257412 | SRA1791874 | Affiliated Eye Hospital of Nanchang University|Department of Ophthalmology | Affiliated Eye Hospital of Nanchang University | B | B | biological fallback assumption | illumina | novaseq_era | unknown | poly_a | unknown | bulk | unknown | unknown | China | 2024-01-25 | Adult | Adult | Eye | Sensory System | |||||||||||||||||||||||||||||||
| 30248 | 30248 | SRR27730925 | SRX23396536 | SRS20257411 | SRP486007 | PRJNA1069290 | Noninvasive light flicker stimulation promotes optic nerve regeneration by activating microglia and enhancing neural plasticity in zebrafish | PRJNA1069290 | Other | In the present study we demonstrated that the optic nerve can spontaneously regenerate and that visual function can be restored post ONC in zebrafish. Forty Hertz light flicker stimulation modulated microglia in the zebrafish retina post ONC promoted dendritic remodeling and axonal regeneration of RGCs and restored visual function. Transcriptomic and qPCR results both indicated that 40 Hz light flicker stimulation increased the expression of genes related to neural plasticity. In general our study showed that 40 Hz light flicker stimulation promotes axonal and dendritic regeneration of RGCs as well as the recovery of visual function which is related to the activation of microglia and enhancement of neural plasticity. | Con+biological replicate 2 | Con 2 | strain:AB strain|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|age:3 month 6 month|dev stage:3 month 6 month|collection date:2023 07|geo loc name:China:Nanchang|sex:pooled male and female|tissue:retina|ID:2|BioSampleModel:Model organism or animal | RNA seq of Danio rerio: retina | M23165691aR1AL1A | M23165691aR1AL1A | Control | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP486007 | Con_2_1.fq.gz Con_2_2.fq.gz | fastq fastq | 7211639400.0 | 24038798.0 | Con 2 1.fq.gz | 0:150 1:150 | A:1979869383;C:1617364490;G:1649637969;T:1964729059;N:38499 | 150 | 150 | 1979869383 | 1617364490 | 1649637969 | 1964729059 | 38499 | SRX23396536 | SRS20257411 | SRA1791874 | Affiliated Eye Hospital of Nanchang University|Department of Ophthalmology | Affiliated Eye Hospital of Nanchang University | B | B | biological fallback assumption | illumina | novaseq_era | unknown | poly_a | unknown | bulk | unknown | unknown | China | 2024-01-25 | Adult | Adult | Eye | Sensory System | |||||||||||||||||||||||||||||||
| 30249 | 30249 | SRR27730926 | SRX23396535 | SRS20257410 | SRP486007 | PRJNA1069290 | Noninvasive light flicker stimulation promotes optic nerve regeneration by activating microglia and enhancing neural plasticity in zebrafish | PRJNA1069290 | Other | In the present study we demonstrated that the optic nerve can spontaneously regenerate and that visual function can be restored post ONC in zebrafish. Forty Hertz light flicker stimulation modulated microglia in the zebrafish retina post ONC promoted dendritic remodeling and axonal regeneration of RGCs and restored visual function. Transcriptomic and qPCR results both indicated that 40 Hz light flicker stimulation increased the expression of genes related to neural plasticity. In general our study showed that 40 Hz light flicker stimulation promotes axonal and dendritic regeneration of RGCs as well as the recovery of visual function which is related to the activation of microglia and enhancement of neural plasticity. | Con+biological replicate 1 | Con 1 | strain:AB strain|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|age:3 month 6 month|dev stage:3 month 6 month|collection date:2023 07|geo loc name:China:Nanchang|sex:pooled male and female|tissue:retina|ID:1|BioSampleModel:Model organism or animal | RNA seq of Danio rerio: retina | M23165689aR1AL1A | M23165689aR1AL1A | Control | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | PAIRED | ILLUMINA | Illumina NovaSeq 6000 | SRP486007 | Con_1_1.fq.gz Con_1_2.fq.gz | fastq fastq | 6217369800.0 | 20724566.0 | Con 1 1.fq.gz | 0:150 1:150 | A:1714156006;C:1391042352;G:1415307378;T:1696831326;N:32738 | 150 | 150 | 1714156006 | 1391042352 | 1415307378 | 1696831326 | 32738 | SRX23396535 | SRS20257410 | SRA1791874 | Affiliated Eye Hospital of Nanchang University|Department of Ophthalmology | Affiliated Eye Hospital of Nanchang University | B | B | biological fallback assumption | illumina | novaseq_era | unknown | poly_a | unknown | bulk | unknown | unknown | China | 2024-01-25 | Adult | Adult | Eye | Sensory System | |||||||||||||||||||||||||||||||
| 33304 | 33304 | SRR29927538 | SRX25421512 | SRS22081668 | SRP521592 | PRJNA1139127 | Laser capture microdissection RNA sequencing of zebrafish optic nerve regeneration | PRJNA1139127 | Other | Zebrafish have the ability to successfully regenerate the connection from the eye to the brain following optic nerve injury in adult. To identify differentially expressed genes in the affected tissues we performed laser capture mRNA sequencing of the retinal ganglion cell layer inner nuclear layer outer nuclear layer and bulk optic nerve in 3 day post optic nerve injury versus naive control animals. The 3 xxx post injury time point was chosen as a time when axons are actively regenerating throughout the optic nerve to the optic chiasm. These data provide a resource for identifying genes expressed in each tissue and their differential expression during optic nerve regeneration. | INL 3d rep2 | MCWR 2019 000138 | strain:wild type|dev stage:adult|collection date:2019 10 18|geo loc name:USA: Wisconsin Milwaukee|sex:male|tissue:retina|treatment:INL 3d rep2|BioSampleModel:Model organism or animal | RNA Seq of Danio rerio: 3 day post optic nerve injury inner nuclear layer | INL 3d rep2 | INL 3d rep2 | SMART Seqv4 | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | PAIRED | ILLUMINA | Illumina HiSeq 2000 | SRP521592 | MCWR_2019_000138_S10_L001_R1_001.fastq.gz MCWR_2019_000138_S10_L001_R2_001.fastq.gz MCWR_2019_000138_S10_L002_R1_001.fastq.gz MCWR_2019_000138_S10_L002_R2_001.fastq.gz | fastq fastq fastq fastq | 12792286105.0 | 42489107.0 | MCWR 2019 000138 S10 L001 R1 001.fastq.gz | 0:150.53 1:150.54 | A:3362192057;C:3017281691;G:3072735924;T:3339916027;N:160406 | 150 | 150 | 3362192057 | 3017281691 | 3072735924 | 3339916027 | 160406 | SRX25421512 | SRS22081668 | SRA1930690 | Medical College of Wisconsin|Cell Biology, Neurobiology, and Anatomy | Medical College of Wisconsin | 2 | 0.95023 | 0.94904 | 0.10306 | 0.10406 | 0.71167 | 0.71305 | 0.46929 | 0.47143 | 149 | 151 | B | B | biological fallback assumption | illumina | hiseq_era | unknown | poly_a | unknown | sc | single_cell_plate | smartseq | United States | 2024-07-23 | Adult | Adult | Eye | Sensory System | ||||||||||||||||||||
| 33305 | 33305 | SRR29927539 | SRX25421511 | SRS22081667 | SRP521592 | PRJNA1139127 | Laser capture microdissection RNA sequencing of zebrafish optic nerve regeneration | PRJNA1139127 | Other | Zebrafish have the ability to successfully regenerate the connection from the eye to the brain following optic nerve injury in adult. To identify differentially expressed genes in the affected tissues we performed laser capture mRNA sequencing of the retinal ganglion cell layer inner nuclear layer outer nuclear layer and bulk optic nerve in 3 day post optic nerve injury versus naive control animals. The 3 xxx post injury time point was chosen as a time when axons are actively regenerating throughout the optic nerve to the optic chiasm. These data provide a resource for identifying genes expressed in each tissue and their differential expression during optic nerve regeneration. | INL 3d rep1 | MCWR 2019 000137 | strain:wild type|dev stage:adult|collection date:2019 10 18|geo loc name:USA: Wisconsin Milwaukee|sex:male|tissue:retina|treatment:INL 3d rep1|BioSampleModel:Model organism or animal | RNA Seq of Danio rerio: 3 day post optic nerve injury inner nuclear layer | INL 3d rep1 | INL 3d rep1 | SMART Seqv4 | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | PAIRED | ILLUMINA | Illumina HiSeq 2000 | SRP521592 | MCWR_2019_000137_S9_L001_R1_001.fastq.gz MCWR_2019_000137_S9_L001_R2_001.fastq.gz MCWR_2019_000137_S9_L002_R1_001.fastq.gz MCWR_2019_000137_S9_L002_R2_001.fastq.gz | fastq fastq fastq fastq | 11290383949.0 | 37500632.0 | MCWR 2019 000137 S9 L001 R1 001.fastq.gz | 0:150.53 1:150.54 | A:2978304239;C:2650227687;G:2709306614;T:2952404306;N:141103 | 150 | 150 | 2978304239 | 2650227687 | 2709306614 | 2952404306 | 141103 | SRX25421511 | SRS22081667 | SRA1930690 | Medical College of Wisconsin|Cell Biology, Neurobiology, and Anatomy | Medical College of Wisconsin | 2 | 0.95037 | 0.94915 | 0.09395 | 0.0939 | 0.71019 | 0.71143 | 0.47425 | 0.46854 | 151 | 150 | B | B | biological fallback assumption | illumina | hiseq_era | unknown | poly_a | unknown | sc | single_cell_plate | smartseq | United States | 2024-07-23 | Adult | Adult | Eye | Sensory System | ||||||||||||||||||||
| 33306 | 33306 | SRR29927540 | SRX25421510 | SRS22081666 | SRP521592 | PRJNA1139127 | Laser capture microdissection RNA sequencing of zebrafish optic nerve regeneration | PRJNA1139127 | Other | Zebrafish have the ability to successfully regenerate the connection from the eye to the brain following optic nerve injury in adult. To identify differentially expressed genes in the affected tissues we performed laser capture mRNA sequencing of the retinal ganglion cell layer inner nuclear layer outer nuclear layer and bulk optic nerve in 3 day post optic nerve injury versus naive control animals. The 3 xxx post injury time point was chosen as a time when axons are actively regenerating throughout the optic nerve to the optic chiasm. These data provide a resource for identifying genes expressed in each tissue and their differential expression during optic nerve regeneration. | INL Control rep3 | MCWR 2019 000136 | strain:wild type|dev stage:adult|collection date:2019 10 18|geo loc name:USA: Wisconsin Milwaukee|sex:male|tissue:retina|treatment:INL Control rep3|BioSampleModel:Model organism or animal | RNA Seq of Danio rerio: naive inner nuclear layer | INL Control rep3 | INL Control rep3 | SMART Seqv4 | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | PAIRED | ILLUMINA | Illumina HiSeq 2000 | SRP521592 | MCWR_2019_000136_S8_L001_R1_001.fastq.gz MCWR_2019_000136_S8_L001_R2_001.fastq.gz MCWR_2019_000136_S8_L002_R1_001.fastq.gz MCWR_2019_000136_S8_L002_R2_001.fastq.gz | fastq fastq fastq fastq | 10926876847.0 | 36289612.0 | MCWR 2019 000136 S8 L001 R1 001.fastq.gz | 0:150.54 1:150.56 | A:2876719537;C:2534137531;G:2628404662;T:2887478941;N:136176 | 150 | 150 | 2876719537 | 2534137531 | 2628404662 | 2887478941 | 136176 | SRX25421510 | SRS22081666 | SRA1930690 | Medical College of Wisconsin|Cell Biology, Neurobiology, and Anatomy | Medical College of Wisconsin | 2 | 0.9413 | 0.94085 | 0.12465 | 0.12554 | 0.71565 | 0.71536 | 0.47866 | 0.47866 | 151 | 151 | B | B | biological fallback assumption | illumina | hiseq_era | unknown | poly_a | unknown | sc | single_cell_plate | smartseq | United States | 2024-07-23 | Adult | Adult | Eye | Sensory System | ||||||||||||||||||||
| 33307 | 33307 | SRR29927541 | SRX25421509 | SRS22081665 | SRP521592 | PRJNA1139127 | Laser capture microdissection RNA sequencing of zebrafish optic nerve regeneration | PRJNA1139127 | Other | Zebrafish have the ability to successfully regenerate the connection from the eye to the brain following optic nerve injury in adult. To identify differentially expressed genes in the affected tissues we performed laser capture mRNA sequencing of the retinal ganglion cell layer inner nuclear layer outer nuclear layer and bulk optic nerve in 3 day post optic nerve injury versus naive control animals. The 3 xxx post injury time point was chosen as a time when axons are actively regenerating throughout the optic nerve to the optic chiasm. These data provide a resource for identifying genes expressed in each tissue and their differential expression during optic nerve regeneration. | INL Control rep2 | MCWR 2019 000135 | strain:wild type|dev stage:adult|collection date:2019 10 18|geo loc name:USA: Wisconsin Milwaukee|sex:male|tissue:retina|treatment:INL Control rep2|BioSampleModel:Model organism or animal | RNA Seq of Danio rerio: naive inner nuclear layer | INL Control rep2 | INL Control rep2 | SMART Seqv4 | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | PAIRED | ILLUMINA | Illumina HiSeq 2000 | SRP521592 | MCWR_2019_000135_S7_L001_R1_001.fastq.gz MCWR_2019_000135_S7_L001_R2_001.fastq.gz MCWR_2019_000135_S7_L002_R1_001.fastq.gz MCWR_2019_000135_S7_L002_R2_001.fastq.gz | fastq fastq fastq fastq | 11015573968.0 | 36586451.0 | MCWR 2019 000135 S7 L001 R1 001.fastq.gz | 0:150.54 1:150.54 | A:2922133009;C:2558945272;G:2631247657;T:2903110333;N:137697 | 150 | 150 | 2922133009 | 2558945272 | 2631247657 | 2903110333 | 137697 | SRX25421509 | SRS22081665 | SRA1930690 | Medical College of Wisconsin|Cell Biology, Neurobiology, and Anatomy | Medical College of Wisconsin | 2 | 0.94458 | 0.94452 | 0.1088 | 0.10943 | 0.71167 | 0.71161 | 0.48464 | 0.48393 | 149 | 151 | B | B | biological fallback assumption | illumina | hiseq_era | unknown | poly_a | unknown | sc | single_cell_plate | smartseq | United States | 2024-07-23 | Adult | Adult | Eye | Sensory System | ||||||||||||||||||||
| 33308 | 33308 | SRR29927542 | SRX25421508 | SRS22081664 | SRP521592 | PRJNA1139127 | Laser capture microdissection RNA sequencing of zebrafish optic nerve regeneration | PRJNA1139127 | Other | Zebrafish have the ability to successfully regenerate the connection from the eye to the brain following optic nerve injury in adult. To identify differentially expressed genes in the affected tissues we performed laser capture mRNA sequencing of the retinal ganglion cell layer inner nuclear layer outer nuclear layer and bulk optic nerve in 3 day post optic nerve injury versus naive control animals. The 3 xxx post injury time point was chosen as a time when axons are actively regenerating throughout the optic nerve to the optic chiasm. These data provide a resource for identifying genes expressed in each tissue and their differential expression during optic nerve regeneration. | INL Control rep1 | MCWR 2019 000134 | strain:wild type|dev stage:adult|collection date:2019 10 18|geo loc name:USA: Wisconsin Milwaukee|sex:male|tissue:retina|treatment:INL Control rep1|BioSampleModel:Model organism or animal | RNA Seq of Danio rerio: naive inner nuclear layer | INL Control rep1 | INL Control rep1 | SMART Seqv4 | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | PAIRED | ILLUMINA | Illumina HiSeq 2000 | SRP521592 | MCWR_2019_000134_S6_L001_R1_001.fastq.gz MCWR_2019_000134_S6_L001_R2_001.fastq.gz MCWR_2019_000134_S6_L002_R1_001.fastq.gz MCWR_2019_000134_S6_L002_R2_001.fastq.gz | fastq fastq fastq fastq | 12065204348.0 | 40074475.0 | MCWR 2019 000134 S6 L001 R1 001.fastq.gz | 0:150.53 1:150.54 | A:3229181479;C:2787700756;G:2849413410;T:3198752940;N:155763 | 150 | 150 | 3229181479 | 2787700756 | 2849413410 | 3198752940 | 155763 | SRX25421508 | SRS22081664 | SRA1930690 | Medical College of Wisconsin|Cell Biology, Neurobiology, and Anatomy | Medical College of Wisconsin | 2 | 0.94249 | 0.94233 | 0.10755 | 0.10774 | 0.71427 | 0.71449 | 0.48361 | 0.48286 | 151 | 150 | B | B | biological fallback assumption | illumina | hiseq_era | unknown | poly_a | unknown | sc | single_cell_plate | smartseq | United States | 2024-07-23 | Adult | Adult | Eye | Sensory System | ||||||||||||||||||||
| 33309 | 33309 | SRR29927543 | SRX25421507 | SRS22081663 | SRP521592 | PRJNA1139127 | Laser capture microdissection RNA sequencing of zebrafish optic nerve regeneration | PRJNA1139127 | Other | Zebrafish have the ability to successfully regenerate the connection from the eye to the brain following optic nerve injury in adult. To identify differentially expressed genes in the affected tissues we performed laser capture mRNA sequencing of the retinal ganglion cell layer inner nuclear layer outer nuclear layer and bulk optic nerve in 3 day post optic nerve injury versus naive control animals. The 3 xxx post injury time point was chosen as a time when axons are actively regenerating throughout the optic nerve to the optic chiasm. These data provide a resource for identifying genes expressed in each tissue and their differential expression during optic nerve regeneration. | GCL 3d rep3 | MCWR 2019 000133 | strain:wild type|dev stage:adult|collection date:2019 10 18|geo loc name:USA: Wisconsin Milwaukee|sex:male|tissue:retina|treatment:GCL 3d rep3|BioSampleModel:Model organism or animal | RNA Seq of Danio rerio: 3 day post optic nerve injury ganglion cell layer | GCL 3d rep3 | GCL 3d rep3 | SMART Seqv4 | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | PAIRED | ILLUMINA | Illumina HiSeq 2000 | SRP521592 | MCWR_2019_000133_S5_L001_R1_001.fastq.gz MCWR_2019_000133_S5_L001_R2_001.fastq.gz MCWR_2019_000133_S5_L002_R1_001.fastq.gz MCWR_2019_000133_S5_L002_R2_001.fastq.gz | fastq fastq fastq fastq | 11567455830.0 | 38423805.0 | MCWR 2019 000133 S5 L001 R1 001.fastq.gz | 0:150.52 1:150.52 | A:3334076845;C:2423637444;G:2488288638;T:3321309876;N:143027 | 150 | 150 | 3334076845 | 2423637444 | 2488288638 | 3321309876 | 143027 | SRX25421507 | SRS22081663 | SRA1930690 | Medical College of Wisconsin|Cell Biology, Neurobiology, and Anatomy | Medical College of Wisconsin | 2 | 0.90351 | 0.90273 | 0.20071 | 0.20036 | 0.7443 | 0.74588 | 0.57805 | 0.58483 | 151 | 151 | B | B | biological fallback assumption | illumina | hiseq_era | unknown | poly_a | unknown | sc | single_cell_plate | smartseq | United States | 2024-07-23 | Adult | Adult | Eye | Sensory System | ||||||||||||||||||||
| 33310 | 33310 | SRR29927544 | SRX25421506 | SRS22081662 | SRP521592 | PRJNA1139127 | Laser capture microdissection RNA sequencing of zebrafish optic nerve regeneration | PRJNA1139127 | Other | Zebrafish have the ability to successfully regenerate the connection from the eye to the brain following optic nerve injury in adult. To identify differentially expressed genes in the affected tissues we performed laser capture mRNA sequencing of the retinal ganglion cell layer inner nuclear layer outer nuclear layer and bulk optic nerve in 3 day post optic nerve injury versus naive control animals. The 3 xxx post injury time point was chosen as a time when axons are actively regenerating throughout the optic nerve to the optic chiasm. These data provide a resource for identifying genes expressed in each tissue and their differential expression during optic nerve regeneration. | GCL 3d rep2 | MCWR 2019 000132 | strain:wild type|dev stage:adult|collection date:2019 10 18|geo loc name:USA: Wisconsin Milwaukee|sex:male|tissue:retina|treatment:GCL 3d rep2|BioSampleModel:Model organism or animal | RNA Seq of Danio rerio: 3 day post optic nerve injury ganglion cell layer | GCL 3d rep2 | GCL 3d rep2 | SMART Seqv4 | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | PAIRED | ILLUMINA | Illumina HiSeq 2000 | SRP521592 | MCWR_2019_000132_S4_L001_R1_001.fastq.gz MCWR_2019_000132_S4_L001_R2_001.fastq.gz MCWR_2019_000132_S4_L002_R1_001.fastq.gz MCWR_2019_000132_S4_L002_R2_001.fastq.gz | fastq fastq fastq fastq | 9928742591.0 | 32979607.0 | MCWR 2019 000132 S4 L001 R1 001.fastq.gz | 0:150.53 1:150.53 | A:2670122365;C:2289339998;G:2333968379;T:2635186637;N:125212 | 150 | 150 | 2670122365 | 2289339998 | 2333968379 | 2635186637 | 125212 | SRX25421506 | SRS22081662 | SRA1930690 | Medical College of Wisconsin|Cell Biology, Neurobiology, and Anatomy | Medical College of Wisconsin | 2 | 0.94453 | 0.9429 | 0.1039 | 0.10476 | 0.72032 | 0.72184 | 0.51703 | 0.51652 | 148 | 149 | B | B | biological fallback assumption | illumina | hiseq_era | unknown | poly_a | unknown | sc | single_cell_plate | smartseq | United States | 2024-07-23 | Adult | Adult | Eye | Sensory System | ||||||||||||||||||||
| 33313 | 33313 | SRR29927547 | SRX25421503 | SRS22081659 | SRP521592 | PRJNA1139127 | Laser capture microdissection RNA sequencing of zebrafish optic nerve regeneration | PRJNA1139127 | Other | Zebrafish have the ability to successfully regenerate the connection from the eye to the brain following optic nerve injury in adult. To identify differentially expressed genes in the affected tissues we performed laser capture mRNA sequencing of the retinal ganglion cell layer inner nuclear layer outer nuclear layer and bulk optic nerve in 3 day post optic nerve injury versus naive control animals. The 3 xxx post injury time point was chosen as a time when axons are actively regenerating throughout the optic nerve to the optic chiasm. These data provide a resource for identifying genes expressed in each tissue and their differential expression during optic nerve regeneration. | GCL 3d rep1 | MCWR 2019 000131 | strain:wild type|dev stage:adult|collection date:2019 10 18|geo loc name:USA: Wisconsin Milwaukee|sex:male|tissue:retina|treatment:GCL 3d rep1|BioSampleModel:Model organism or animal | RNA Seq of Danio rerio: 3 day post optic nerve injury ganglion cell layer | GCL 3d rep1 | GCL 3d rep1 | SMART Seqv4 | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | PAIRED | ILLUMINA | Illumina HiSeq 2000 | SRP521592 | MCWR_2019_000131_S3_L001_R1_001.fastq.gz MCWR_2019_000131_S3_L001_R2_001.fastq.gz MCWR_2019_000131_S3_L002_R1_001.fastq.gz MCWR_2019_000131_S3_L002_R2_001.fastq.gz | fastq fastq fastq fastq | 11040747879.0 | 36674133.0 | MCWR 2019 000131 S3 L001 R1 001.fastq.gz | 0:150.52 1:150.53 | A:3033865749;C:2483236067;G:2540109846;T:2983395404;N:140813 | 150 | 150 | 3033865749 | 2483236067 | 2540109846 | 2983395404 | 140813 | SRX25421503 | SRS22081659 | SRA1930690 | Medical College of Wisconsin|Cell Biology, Neurobiology, and Anatomy | Medical College of Wisconsin | 2 | 0.9328 | 0.93075 | 0.12255 | 0.12345 | 0.72675 | 0.72949 | 0.52776 | 0.53024 | 150 | 149 | B | B | biological fallback assumption | illumina | hiseq_era | unknown | poly_a | unknown | sc | single_cell_plate | smartseq | United States | 2024-07-23 | Adult | Adult | Eye | Sensory System | ||||||||||||||||||||
| 33318 | 33318 | SRR29927552 | SRX25421498 | SRS22081654 | SRP521592 | PRJNA1139127 | Laser capture microdissection RNA sequencing of zebrafish optic nerve regeneration | PRJNA1139127 | Other | Zebrafish have the ability to successfully regenerate the connection from the eye to the brain following optic nerve injury in adult. To identify differentially expressed genes in the affected tissues we performed laser capture mRNA sequencing of the retinal ganglion cell layer inner nuclear layer outer nuclear layer and bulk optic nerve in 3 day post optic nerve injury versus naive control animals. The 3 xxx post injury time point was chosen as a time when axons are actively regenerating throughout the optic nerve to the optic chiasm. These data provide a resource for identifying genes expressed in each tissue and their differential expression during optic nerve regeneration. | ONL 3d rep3 | MCWR 2019 000145 | strain:wild type|dev stage:adult|collection date:2019 10 18|geo loc name:USA: Wisconsin Milwaukee|sex:male|tissue:retina|treatment:ONL 3d rep3|BioSampleModel:Model organism or animal | RNA Seq of Danio rerio: 3 day post optic nerve injury outer nuclear layer | ONL 3d rep3 | ONL 3d rep3 | SMART Seqv4 | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | PAIRED | ILLUMINA | Illumina HiSeq 2000 | SRP521592 | MCWR_2019_000145_S16_L001_R1_001.fastq.gz MCWR_2019_000145_S16_L001_R2_001.fastq.gz MCWR_2019_000145_S16_L002_R1_001.fastq.gz MCWR_2019_000145_S16_L002_R2_001.fastq.gz | fastq fastq fastq fastq | 11434401733.0 | 37973868.0 | MCWR 2019 000145 S16 L001 R1 001.fastq.gz | 0:150.56 1:150.55 | A:2952068036;C:2779158705;G:2786796140;T:2916234156;N:144696 | 150 | 150 | 2952068036 | 2779158705 | 2786796140 | 2916234156 | 144696 | SRX25421498 | SRS22081654 | SRA1930690 | Medical College of Wisconsin|Cell Biology, Neurobiology, and Anatomy | Medical College of Wisconsin | 2 | 0.95416 | 0.95385 | 0.07766 | 0.07806 | 0.79024 | 0.78989 | 0.40138 | 0.42872 | 150 | 151 | B | B | biological fallback assumption | illumina | hiseq_era | unknown | poly_a | unknown | sc | single_cell_plate | smartseq | United States | 2024-07-23 | Adult | Adult | Eye | Sensory System | ||||||||||||||||||||
| 33319 | 33319 | SRR29927553 | SRX25421497 | SRS22081653 | SRP521592 | PRJNA1139127 | Laser capture microdissection RNA sequencing of zebrafish optic nerve regeneration | PRJNA1139127 | Other | Zebrafish have the ability to successfully regenerate the connection from the eye to the brain following optic nerve injury in adult. To identify differentially expressed genes in the affected tissues we performed laser capture mRNA sequencing of the retinal ganglion cell layer inner nuclear layer outer nuclear layer and bulk optic nerve in 3 day post optic nerve injury versus naive control animals. The 3 xxx post injury time point was chosen as a time when axons are actively regenerating throughout the optic nerve to the optic chiasm. These data provide a resource for identifying genes expressed in each tissue and their differential expression during optic nerve regeneration. | ONL 3d rep2 | MCWR 2019 000144 | strain:wild type|dev stage:adult|collection date:2019 10 18|geo loc name:USA: Wisconsin Milwaukee|sex:male|tissue:retina|treatment:ONL 3d rep2|BioSampleModel:Model organism or animal | RNA Seq of Danio rerio: 3 day post optic nerve injury outer nuclear layer | ONL 3d rep2 | ONL 3d rep2 | SMART Seqv4 | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | PAIRED | ILLUMINA | Illumina HiSeq 2000 | SRP521592 | MCWR_2019_000144_S15_L002_R2_001.fastq.gz MCWR_2019_000144_S15_L002_R1_001.fastq.gz MCWR_2019_000144_S15_L001_R2_001.fastq.gz MCWR_2019_000144_S15_L001_R1_001.fastq.gz | fastq fastq fastq fastq | 10784131193.0 | 35816254.0 | MCWR 2019 000144 S15 L001 R1 001.fastq.gz | 0:150.55 1:150.55 | A:2770521598;C:2622163802;G:2642603869;T:2748709177;N:132747 | 150 | 150 | 2770521598 | 2622163802 | 2642603869 | 2748709177 | 132747 | SRX25421497 | SRS22081653 | SRA1930690 | Medical College of Wisconsin|Cell Biology, Neurobiology, and Anatomy | Medical College of Wisconsin | 2 | 0.95823 | 0.9574 | 0.06867 | 0.06894 | 0.77055 | 0.77234 | 0.43067 | 0.43052 | 149 | 151 | B | B | biological fallback assumption | illumina | hiseq_era | unknown | poly_a | unknown | sc | single_cell_plate | smartseq | United States | 2024-07-23 | Adult | Adult | Eye | Sensory System | ||||||||||||||||||||
| 33320 | 33320 | SRR29927554 | SRX25421496 | SRS22081652 | SRP521592 | PRJNA1139127 | Laser capture microdissection RNA sequencing of zebrafish optic nerve regeneration | PRJNA1139127 | Other | Zebrafish have the ability to successfully regenerate the connection from the eye to the brain following optic nerve injury in adult. To identify differentially expressed genes in the affected tissues we performed laser capture mRNA sequencing of the retinal ganglion cell layer inner nuclear layer outer nuclear layer and bulk optic nerve in 3 day post optic nerve injury versus naive control animals. The 3 xxx post injury time point was chosen as a time when axons are actively regenerating throughout the optic nerve to the optic chiasm. These data provide a resource for identifying genes expressed in each tissue and their differential expression during optic nerve regeneration. | ONL 3d rep1 | MCWR 2019 000143 | strain:wild type|dev stage:adult|collection date:2019 10 18|geo loc name:USA: Wisconsin Milwaukee|sex:male|tissue:retina|treatment:ONL 3d rep1|BioSampleModel:Model organism or animal | RNA Seq of Danio rerio: 3 day post optic nerve injury outer nuclear layer | ONL 3d rep1 | ONL 3d rep1 | SMART Seqv4 | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | PAIRED | ILLUMINA | Illumina HiSeq 2000 | SRP521592 | MCWR_2019_000143_S14_L001_R1_001.fastq.gz MCWR_2019_000143_S14_L001_R2_001.fastq.gz MCWR_2019_000143_S14_L002_R1_001.fastq.gz MCWR_2019_000143_S14_L002_R2_001.fastq.gz | fastq fastq fastq fastq | 10414222985.0 | 34587231.0 | MCWR 2019 000143 S14 L001 R1 001.fastq.gz | 0:150.55 1:150.55 | A:2640548677;C:2562227240;G:2582839755;T:2628478265;N:129048 | 150 | 150 | 2640548677 | 2562227240 | 2582839755 | 2628478265 | 129048 | SRX25421496 | SRS22081652 | SRA1930690 | Medical College of Wisconsin|Cell Biology, Neurobiology, and Anatomy | Medical College of Wisconsin | 2 | 0.95893 | 0.95898 | 0.06693 | 0.06725 | 0.78216 | 0.7833 | 0.41018 | 0.40065 | 151 | 151 | B | B | biological fallback assumption | illumina | hiseq_era | unknown | poly_a | unknown | sc | single_cell_plate | smartseq | United States | 2024-07-23 | Adult | Adult | Eye | Sensory System | ||||||||||||||||||||
| 33321 | 33321 | SRR29927555 | SRX25421495 | SRS22081651 | SRP521592 | PRJNA1139127 | Laser capture microdissection RNA sequencing of zebrafish optic nerve regeneration | PRJNA1139127 | Other | Zebrafish have the ability to successfully regenerate the connection from the eye to the brain following optic nerve injury in adult. To identify differentially expressed genes in the affected tissues we performed laser capture mRNA sequencing of the retinal ganglion cell layer inner nuclear layer outer nuclear layer and bulk optic nerve in 3 day post optic nerve injury versus naive control animals. The 3 xxx post injury time point was chosen as a time when axons are actively regenerating throughout the optic nerve to the optic chiasm. These data provide a resource for identifying genes expressed in each tissue and their differential expression during optic nerve regeneration. | ONL Control rep3 | MCWR 2019 000142 | strain:wild type|dev stage:adult|collection date:2019 10 18|geo loc name:USA: Wisconsin Milwaukee|sex:male|tissue:retina|treatment:ONL Control rep3|BioSampleModel:Model organism or animal | RNA Seq of Danio rerio: naive outer nuclear layer | ONL Control rep3 | ONL Control rep3 | SMART Seqv4 | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | PAIRED | ILLUMINA | Illumina HiSeq 2000 | SRP521592 | MCWR_2019_000142_S13_L001_R1_001.fastq.gz MCWR_2019_000142_S13_L001_R2_001.fastq.gz MCWR_2019_000142_S13_L002_R1_001.fastq.gz MCWR_2019_000142_S13_L002_R2_001.fastq.gz | fastq fastq fastq fastq | 10611460744.0 | 35241917.0 | MCWR 2019 000142 S13 L001 R1 001.fastq.gz | 0:150.55 1:150.55 | A:2729659216;C:2572974863;G:2597112533;T:2711584081;N:130051 | 150 | 150 | 2729659216 | 2572974863 | 2597112533 | 2711584081 | 130051 | SRX25421495 | SRS22081651 | SRA1930690 | Medical College of Wisconsin|Cell Biology, Neurobiology, and Anatomy | Medical College of Wisconsin | 2 | 0.95524 | 0.95438 | 0.08233 | 0.08236 | 0.76654 | 0.76682 | 0.41657 | 0.41626 | 151 | 150 | B | B | biological fallback assumption | illumina | hiseq_era | unknown | poly_a | unknown | sc | single_cell_plate | smartseq | United States | 2024-07-23 | Adult | Adult | Eye | Sensory System | ||||||||||||||||||||
| 33322 | 33322 | SRR29927556 | SRX25421494 | SRS22081650 | SRP521592 | PRJNA1139127 | Laser capture microdissection RNA sequencing of zebrafish optic nerve regeneration | PRJNA1139127 | Other | Zebrafish have the ability to successfully regenerate the connection from the eye to the brain following optic nerve injury in adult. To identify differentially expressed genes in the affected tissues we performed laser capture mRNA sequencing of the retinal ganglion cell layer inner nuclear layer outer nuclear layer and bulk optic nerve in 3 day post optic nerve injury versus naive control animals. The 3 xxx post injury time point was chosen as a time when axons are actively regenerating throughout the optic nerve to the optic chiasm. These data provide a resource for identifying genes expressed in each tissue and their differential expression during optic nerve regeneration. | ONL Control rep2 | MCWR 2019 000141 | strain:wild type|dev stage:adult|collection date:2019 10 18|geo loc name:USA: Wisconsin Milwaukee|sex:male|tissue:retina|treatment:ONL Control rep2|BioSampleModel:Model organism or animal | RNA Seq of Danio rerio: naive outer nuclear layer | ONL Control rep2 | ONL Control rep2 | SMART Seqv4 | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | PAIRED | ILLUMINA | Illumina HiSeq 2000 | SRP521592 | MCWR_2019_000141_S12_L001_R1_001.fastq.gz MCWR_2019_000141_S12_L001_R2_001.fastq.gz MCWR_2019_000141_S12_L002_R1_001.fastq.gz MCWR_2019_000141_S12_L002_R2_001.fastq.gz | fastq fastq fastq fastq | 12296763008.0 | 40838426.0 | MCWR 2019 000141 S12 L001 R1 001.fastq.gz | 0:150.55 1:150.55 | A:3157494503;C:2976873172;G:3025100891;T:3137136259;N:158183 | 150 | 150 | 3157494503 | 2976873172 | 3025100891 | 3137136259 | 158183 | SRX25421494 | SRS22081650 | SRA1930690 | Medical College of Wisconsin|Cell Biology, Neurobiology, and Anatomy | Medical College of Wisconsin | 2 | 0.9531 | 0.95203 | 0.08284 | 0.08254 | 0.76816 | 0.76879 | 0.40735 | 0.41583 | 150 | 151 | B | B | biological fallback assumption | illumina | hiseq_era | unknown | poly_a | unknown | sc | single_cell_plate | smartseq | United States | 2024-07-23 | Adult | Adult | Eye | Sensory System | ||||||||||||||||||||
| 33323 | 33323 | SRR29927557 | SRX25421493 | SRS22081649 | SRP521592 | PRJNA1139127 | Laser capture microdissection RNA sequencing of zebrafish optic nerve regeneration | PRJNA1139127 | Other | Zebrafish have the ability to successfully regenerate the connection from the eye to the brain following optic nerve injury in adult. To identify differentially expressed genes in the affected tissues we performed laser capture mRNA sequencing of the retinal ganglion cell layer inner nuclear layer outer nuclear layer and bulk optic nerve in 3 day post optic nerve injury versus naive control animals. The 3 xxx post injury time point was chosen as a time when axons are actively regenerating throughout the optic nerve to the optic chiasm. These data provide a resource for identifying genes expressed in each tissue and their differential expression during optic nerve regeneration. | ONL Control rep1 | MCWR 2019 000140 | strain:wild type|dev stage:adult|collection date:2019 10 18|geo loc name:USA: Wisconsin Milwaukee|sex:male|tissue:retina|treatment:ONL Control rep1|BioSampleModel:Model organism or animal | RNA Seq of Danio rerio: naive outer nuclear layer | ONL Control rep1 | ONL Control rep1 | SMART Seqv4 | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | PAIRED | ILLUMINA | Illumina HiSeq 2000 | SRP521592 | MCWR_2019_000140_S11_L001_R1_001.fastq.gz MCWR_2019_000140_S11_L001_R2_001.fastq.gz MCWR_2019_000140_S11_L002_R1_001.fastq.gz MCWR_2019_000140_S11_L002_R2_001.fastq.gz | fastq fastq fastq fastq | 12797441059.0 | 42498374.0 | MCWR 2019 000140 S11 L001 R1 001.fastq.gz | 0:150.56 1:150.56 | A:3214435348;C:3160301138;G:3218337754;T:3204200743;N:166076 | 150 | 150 | 3214435348 | 3160301138 | 3218337754 | 3204200743 | 166076 | SRX25421493 | SRS22081649 | SRA1930690 | Medical College of Wisconsin|Cell Biology, Neurobiology, and Anatomy | Medical College of Wisconsin | 2 | 0.95836 | 0.95804 | 0.0672 | 0.06787 | 0.79255 | 0.7933 | 0.37626 | 0.37493 | 149 | 151 | B | B | biological fallback assumption | illumina | hiseq_era | unknown | poly_a | unknown | sc | single_cell_plate | smartseq | United States | 2024-07-23 | Adult | Adult | Eye | Sensory System | ||||||||||||||||||||
| 33324 | 33324 | SRR29927558 | SRX25421492 | SRS22081648 | SRP521592 | PRJNA1139127 | Laser capture microdissection RNA sequencing of zebrafish optic nerve regeneration | PRJNA1139127 | Other | Zebrafish have the ability to successfully regenerate the connection from the eye to the brain following optic nerve injury in adult. To identify differentially expressed genes in the affected tissues we performed laser capture mRNA sequencing of the retinal ganglion cell layer inner nuclear layer outer nuclear layer and bulk optic nerve in 3 day post optic nerve injury versus naive control animals. The 3 xxx post injury time point was chosen as a time when axons are actively regenerating throughout the optic nerve to the optic chiasm. These data provide a resource for identifying genes expressed in each tissue and their differential expression during optic nerve regeneration. | GCL Control rep2 | MCWR 2019 000129 | strain:wild type|dev stage:adult|collection date:2019 10 18|geo loc name:USA: Wisconsin Milwaukee|sex:male|tissue:retina|treatment:GCL Control rep2|BioSampleModel:Model organism or animal | RNA Seq of Danio rerio: naive ganglion cell layer | GCL Control rep2 | GCL Control rep2 | SMART Seqv4 | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | PAIRED | ILLUMINA | Illumina HiSeq 2000 | SRP521592 | MCWR_2019_000129_S2_L001_R1_001.fastq.gz MCWR_2019_000129_S2_L001_R2_001.fastq.gz MCWR_2019_000129_S2_L002_R1_001.fastq.gz MCWR_2019_000129_S2_L002_R2_001.fastq.gz | fastq fastq fastq fastq | 9904585990.0 | 32898356.0 | MCWR 2019 000129 S2 L001 R1 001.fastq.gz | 0:150.53 1:150.54 | A:2686769990;C:2251531625;G:2320304039;T:2645855407;N:124929 | 150 | 150 | 2686769990 | 2251531625 | 2320304039 | 2645855407 | 124929 | SRX25421492 | SRS22081648 | SRA1930690 | Medical College of Wisconsin|Cell Biology, Neurobiology, and Anatomy | Medical College of Wisconsin | 2 | 0.93013 | 0.93029 | 0.10617 | 0.10657 | 0.74712 | 0.74757 | 0.56746 | 0.56517 | 150 | 151 | B | B | biological fallback assumption | illumina | hiseq_era | unknown | poly_a | unknown | sc | single_cell_plate | smartseq | United States | 2024-07-23 | Adult | Adult | Eye | Sensory System | ||||||||||||||||||||
| 33325 | 33325 | SRR29927559 | SRX25421491 | SRS22081647 | SRP521592 | PRJNA1139127 | Laser capture microdissection RNA sequencing of zebrafish optic nerve regeneration | PRJNA1139127 | Other | Zebrafish have the ability to successfully regenerate the connection from the eye to the brain following optic nerve injury in adult. To identify differentially expressed genes in the affected tissues we performed laser capture mRNA sequencing of the retinal ganglion cell layer inner nuclear layer outer nuclear layer and bulk optic nerve in 3 day post optic nerve injury versus naive control animals. The 3 xxx post injury time point was chosen as a time when axons are actively regenerating throughout the optic nerve to the optic chiasm. These data provide a resource for identifying genes expressed in each tissue and their differential expression during optic nerve regeneration. | GCL Control rep1 | MCWR 2019 000128 | strain:wild type|dev stage:adult|collection date:2019 10 18|geo loc name:USA: Wisconsin Milwaukee|sex:male|tissue:retina|treatment:GCL Control rep1|BioSampleModel:Model organism or animal | RNA Seq of Danio rerio: naive ganglion cell layer | GCL Control rep1 | GCL Control rep1 | SMART Seqv4 | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | PAIRED | ILLUMINA | Illumina HiSeq 2000 | SRP521592 | MCWR_2019_000128_S1_L001_R1_001.fastq.gz MCWR_2019_000128_S1_L001_R2_001.fastq.gz MCWR_2019_000128_S1_L002_R1_001.fastq.gz MCWR_2019_000128_S1_L002_R2_001.fastq.gz | fastq fastq fastq fastq | 11199698460.0 | 37200254.0 | MCWR 2019 000128 S1 L001 R1 001.fastq.gz | 0:150.53 1:150.53 | A:3065207886;C:2519670393;G:2596314938;T:3018365726;N:139517 | 150 | 150 | 3065207886 | 2519670393 | 2596314938 | 3018365726 | 139517 | SRX25421491 | SRS22081647 | SRA1930690 | Medical College of Wisconsin|Cell Biology, Neurobiology, and Anatomy | Medical College of Wisconsin | 2 | 0.93213 | 0.93206 | 0.13073 | 0.13125 | 0.7305 | 0.73079 | 0.5518 | 0.55133 | 149 | 151 | B | B | biological fallback assumption | illumina | hiseq_era | unknown | poly_a | unknown | sc | single_cell_plate | smartseq | United States | 2024-07-23 | Adult | Adult | Eye | Sensory System | ||||||||||||||||||||
| 67572 | 67572 | SRR17224783 | SRX13404158 | SRS11307154 | SRP350669 | PRJNA788440 | Danio rerio Raw sequence reads | PRJNA788440 | Whole Genome Sequencing | Danio rerio Raw sequence reads of eyes and skin | zebrafisheye | breed:AB|age:30days|sex:pooled male and female|tissue:eyes|BioSampleModel:Model organism or animal | RNAseq of zebrafish | eye mut 1 30dpf | eye mut 1 30dpf | RNAseq of eye | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina NovaSeq 6000 | SRP350669 | eye_mut_1_30dpf.R1.fastq.gz eye_mut_1_30dpf.R2.fastq.gz | fastq fastq | 6647236232.0 | 22010716.0 | eye mut 1 30dpf.R1.fastq.gz | 0:151 1:151 | A:1828748374;C:1496039359;G:1520471614;T:1801966254;N:10631 | 151 | 151 | 1828748374 | 1496039359 | 1520471614 | 1801966254 | 10631 | SRX13404158 | SRS11307154 | SRA1343194 | Huazhong Agricultural University|College of Fisheries | Huazhong Agricultural University | 2 | 0.91773 | 0.91812 | 0.11227 | 0.11178 | 0.6687 | 0.66931 | 0.47877 | 0.47805 | 151 | 151 | B | B | biological fallback assumption | illumina | novaseq_era | unknown | poly_a | unknown | bulk | unknown | unknown | China | 2021-12-14 | Juvenile | Juvenile | Eye | Sensory System | |||||||||||||||||||||
| 67573 | 67573 | SRR17224784 | SRX13404157 | SRS11307154 | SRP350669 | PRJNA788440 | Danio rerio Raw sequence reads | PRJNA788440 | Whole Genome Sequencing | Danio rerio Raw sequence reads of eyes and skin | zebrafisheye | breed:AB|age:30days|sex:pooled male and female|tissue:eyes|BioSampleModel:Model organism or animal | RNAseq of zebrafish | eye wt 3 30dpf | eye wt 3 30dpf | RNAseq of eye | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina NovaSeq 6000 | SRP350669 | eye_wt_3_30dpf.R1.fastq.gz eye_wt_3_30dpf.R2.fastq.gz | fastq fastq | 6665043964.0 | 22069682.0 | eye wt 3 30dpf.R1.fastq.gz | 0:151 1:151 | A:1837907813;C:1496332954;G:1517206099;T:1813585697;N:11401 | 151 | 151 | 1837907813 | 1496332954 | 1517206099 | 1813585697 | 11401 | SRX13404157 | SRS11307154 | SRA1343194 | Huazhong Agricultural University|College of Fisheries | Huazhong Agricultural University | 2 | 0.92328 | 0.92376 | 0.11972 | 0.11905 | 0.69002 | 0.69039 | 0.48654 | 0.48588 | 151 | 151 | B | B | biological fallback assumption | illumina | novaseq_era | unknown | poly_a | unknown | bulk | unknown | unknown | China | 2021-12-14 | Juvenile | Juvenile | Eye | Sensory System | |||||||||||||||||||||
| 67574 | 67574 | SRR17224785 | SRX13404156 | SRS11307154 | SRP350669 | PRJNA788440 | Danio rerio Raw sequence reads | PRJNA788440 | Whole Genome Sequencing | Danio rerio Raw sequence reads of eyes and skin | zebrafisheye | breed:AB|age:30days|sex:pooled male and female|tissue:eyes|BioSampleModel:Model organism or animal | RNAseq of zebrafish | eye wt 2 30dpf | eye wt 2 30dpf | RNAseq of eye | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina NovaSeq 6000 | SRP350669 | eye_wt_2_30dpf.R1.fastq.gz eye_wt_2_30dpf.R2.fastq.gz | fastq fastq | 7313252536.0 | 24216068.0 | eye wt 2 30dpf.R1.fastq.gz | 0:151 1:151 | A:2005760880;C:1644712229;G:1691008059;T:1971759242;N:12126 | 151 | 151 | 2005760880 | 1644712229 | 1691008059 | 1971759242 | 12126 | SRX13404156 | SRS11307154 | SRA1343194 | Huazhong Agricultural University|College of Fisheries | Huazhong Agricultural University | 2 | 0.92458 | 0.92267 | 0.11276 | 0.11098 | 0.68834 | 0.6939 | 0.48994 | 0.48977 | 151 | 151 | B | B | biological fallback assumption | illumina | novaseq_era | unknown | poly_a | unknown | bulk | unknown | unknown | China | 2021-12-14 | Juvenile | Juvenile | Eye | Sensory System | |||||||||||||||||||||
| 67575 | 67575 | SRR17224786 | SRX13404155 | SRS11307154 | SRP350669 | PRJNA788440 | Danio rerio Raw sequence reads | PRJNA788440 | Whole Genome Sequencing | Danio rerio Raw sequence reads of eyes and skin | zebrafisheye | breed:AB|age:30days|sex:pooled male and female|tissue:eyes|BioSampleModel:Model organism or animal | RNAseq of zebrafish | eye wt 1 30dpf | eye wt 1 30dpf | RNAseq of eye | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina NovaSeq 6000 | SRP350669 | eye_wt_1_30dpf.R1.fastq.gz eye_wt_1_30dpf.R2.fastq.gz | fastq fastq | 7374231470.0 | 24417985.0 | eye wt 1 30dpf.R1.fastq.gz | 0:151 1:151 | A:2032283041;C:1657026689;G:1681898985;T:2003011689;N:11066 | 151 | 151 | 2032283041 | 1657026689 | 1681898985 | 2003011689 | 11066 | SRX13404155 | SRS11307154 | SRA1343194 | Huazhong Agricultural University|College of Fisheries | Huazhong Agricultural University | 2 | 0.92217 | 0.92217 | 0.11433 | 0.11344 | 0.69045 | 0.69087 | 0.48935 | 0.47534 | 151 | 151 | B | B | biological fallback assumption | illumina | novaseq_era | unknown | poly_a | unknown | bulk | unknown | unknown | China | 2021-12-14 | Juvenile | Juvenile | Eye | Sensory System | |||||||||||||||||||||
| 67580 | 67580 | SRR17224791 | SRX13404150 | SRS11307154 | SRP350669 | PRJNA788440 | Danio rerio Raw sequence reads | PRJNA788440 | Whole Genome Sequencing | Danio rerio Raw sequence reads of eyes and skin | zebrafisheye | breed:AB|age:30days|sex:pooled male and female|tissue:eyes|BioSampleModel:Model organism or animal | RNAseq of zebrafish | eye mut 3 30dpf | eye mut 3 30dpf | RNAseq of eye | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina NovaSeq 6000 | SRP350669 | eye_mut_3_30dpf.R1.fastq.gz eye_mut_3_30dpf.R2.fastq.gz | fastq fastq | 7370863566.0 | 24406833.0 | eye mut 3 30dpf.R1.fastq.gz | 0:151 1:151 | A:2035593457;C:1650415961;G:1678944830;T:2005897248;N:12070 | 151 | 151 | 2035593457 | 1650415961 | 1678944830 | 2005897248 | 12070 | SRX13404150 | SRS11307154 | SRA1343194 | Huazhong Agricultural University|College of Fisheries | Huazhong Agricultural University | 2 | 0.9169 | 0.91717 | 0.11546 | 0.11616 | 0.66249 | 0.66312 | 0.47895 | 0.47697 | 151 | 151 | B | B | biological fallback assumption | illumina | novaseq_era | unknown | poly_a | unknown | bulk | unknown | unknown | China | 2021-12-14 | Juvenile | Juvenile | Eye | Sensory System | |||||||||||||||||||||
| 67581 | 67581 | SRR17224792 | SRX13404149 | SRS11307154 | SRP350669 | PRJNA788440 | Danio rerio Raw sequence reads | PRJNA788440 | Whole Genome Sequencing | Danio rerio Raw sequence reads of eyes and skin | zebrafisheye | breed:AB|age:30days|sex:pooled male and female|tissue:eyes|BioSampleModel:Model organism or animal | RNAseq of zebrafish | eye mut 2 30dpf | eye mut 2 30dpf | RNAseq of eye | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina NovaSeq 6000 | SRP350669 | eye_mut_2_30dpf.R1.fastq.gz eye_mut_2_30dpf.R2.fastq.gz | fastq fastq | 6025971496.0 | 19953548.0 | eye mut 2 30dpf.R1.fastq.gz | 0:151 1:151 | A:1659612510;C:1355527081;G:1377376165;T:1633446020;N:9720 | 151 | 151 | 1659612510 | 1355527081 | 1377376165 | 1633446020 | 9720 | SRX13404149 | SRS11307154 | SRA1343194 | Huazhong Agricultural University|College of Fisheries | Huazhong Agricultural University | 2 | 0.91909 | 0.92042 | 0.11187 | 0.11181 | 0.66884 | 0.66985 | 0.48856 | 0.49166 | 151 | 151 | B | B | biological fallback assumption | illumina | novaseq_era | unknown | poly_a | unknown | bulk | unknown | unknown | China | 2021-12-14 | Juvenile | Juvenile | Eye | Sensory System |
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CREATE TABLE run_metadata("run.accession" VARCHAR, "experiment.accession" VARCHAR, "sample.accession" VARCHAR, "study.accession" VARCHAR, bioproject VARCHAR, "study.title" VARCHAR, "study.alias" VARCHAR, "study.type" VARCHAR, "study.abstract" VARCHAR, "study.attributes" VARCHAR, "study.PMIDs" VARCHAR, "sample.description" VARCHAR, "sample.title" VARCHAR, "sample.alias" VARCHAR, "sample.centername" VARCHAR, "sample.attributes" VARCHAR, "GEOsample.title" VARCHAR, "GEOsample.dataprocessing" VARCHAR, "GEOsample.source" VARCHAR, "GEOsample.treatmentprotocol" VARCHAR, "GEOsample.extractprotocol" VARCHAR, "GEOsample.growthprotocol" VARCHAR, "GEOsample.characteristics" VARCHAR, "GEOsample.accession" VARCHAR, "experiment.title" VARCHAR, "experiment.alias" VARCHAR, "experiment.library_name" VARCHAR, "experiment.design_description" VARCHAR, "experiment.library_construction_protocol" VARCHAR, "experiment.attributes" VARCHAR, "experiment.library_strategy" VARCHAR, "experiment.library_source" VARCHAR, "experiment.library_selection" VARCHAR, "experiment.library_layout" VARCHAR, "experiment.platform" VARCHAR, "experiment.instrument_model" VARCHAR, "experiment.spot_descriptor" VARCHAR, "experiment.study_ref" VARCHAR, "run.title" VARCHAR, "run.attributes" VARCHAR, "run.filename" VARCHAR, "run.semantic_name" VARCHAR, "run.total_bases" DOUBLE, "run.total_spots" DOUBLE, "run.alias" VARCHAR, "run.read_lengths" VARCHAR, "run.base_counts" VARCHAR, "run.r1_length" BIGINT, "run.r2_length" BIGINT, "run.r3_length" BIGINT, "run.r4_length" BIGINT, "run.Acount" BIGINT, "run.Ccount" BIGINT, "run.Gcount" BIGINT, "run.Tcount" BIGINT, "run.Ncount" BIGINT, "run.experiment" VARCHAR, "run.pool_member" VARCHAR, "submission.accession" VARCHAR, "submission.srasource" VARCHAR, "submission.bioprojectsource" VARCHAR, "seqdetective.n_mates" BIGINT, "seqdetective.mapping_rate.mate1" DOUBLE, "seqdetective.mapping_rate.mate2" DOUBLE, "seqdetective.nofeature_rate.mate1" DOUBLE, "seqdetective.nofeature_rate.mate2" DOUBLE, "seqdetective.sparsity.mate1" DOUBLE, "seqdetective.sparsity.mate2" DOUBLE, "seqdetective.pos_strand_rate.mate1" DOUBLE, "seqdetective.pos_strand_rate.mate2" DOUBLE, "seqdetective.readlen.mate1" BIGINT, "seqdetective.readlen.mate2" BIGINT, "seqdetective.judgement.mate1" VARCHAR, "seqdetective.judgement.mate2" VARCHAR, "seqdetective.judgement.reason" VARCHAR, platform_family VARCHAR, instrument_generation VARCHAR, read_bias VARCHAR, selection_class VARCHAR, prep_kit VARCHAR, sc_or_bulk VARCHAR, tech_class VARCHAR, technology VARCHAR, tech_variant VARCHAR, "submission.bioprojectsource.country" VARCHAR, earliest_date DATE, devstage_curation VARCHAR, devstage_curation_coarse VARCHAR, tissue_curation VARCHAR, tissue_curation_coarse VARCHAR);;
CREATE INDEX idx_run_bioproject ON run_metadata(bioproject);;
CREATE INDEX idx_run_run_accession ON run_metadata("run.accession");;