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542 rows where experiment.library_layout = "SINGLE" and experiment.library_strategy = "miRNA-Seq"

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tissue_curation >10

devstage_curation >10

experiment.library_selection 8

devstage_curation_coarse 6

experiment.platform 4

technology 3

experiment.library_strategy 1

  • miRNA-Seq · 542 ✖

experiment.library_source 1

experiment.library_layout 1

  • SINGLE · 542 ✖
Link rowid ▼ run.accession experiment.accession sample.accession study.accession bioproject study.title study.alias study.type study.abstract study.attributes study.PMIDs sample.description sample.title sample.alias sample.centername sample.attributes GEOsample.title GEOsample.dataprocessing GEOsample.source GEOsample.treatmentprotocol GEOsample.extractprotocol GEOsample.growthprotocol GEOsample.characteristics GEOsample.accession experiment.title experiment.alias experiment.library_name experiment.design_description experiment.library_construction_protocol experiment.attributes experiment.library_strategy experiment.library_source experiment.library_selection experiment.library_layout experiment.platform experiment.instrument_model experiment.spot_descriptor experiment.study_ref run.title run.attributes run.filename run.semantic_name run.total_bases run.total_spots run.alias run.read_lengths run.base_counts run.r1_length run.r2_length run.r3_length run.r4_length run.Acount run.Ccount run.Gcount run.Tcount run.Ncount run.experiment run.pool_member submission.accession submission.srasource submission.bioprojectsource seqdetective.n_mates seqdetective.mapping_rate.mate1 seqdetective.mapping_rate.mate2 seqdetective.nofeature_rate.mate1 seqdetective.nofeature_rate.mate2 seqdetective.sparsity.mate1 seqdetective.sparsity.mate2 seqdetective.pos_strand_rate.mate1 seqdetective.pos_strand_rate.mate2 seqdetective.readlen.mate1 seqdetective.readlen.mate2 seqdetective.judgement.mate1 seqdetective.judgement.mate2 seqdetective.judgement.reason platform_family instrument_generation read_bias selection_class prep_kit sc_or_bulk tech_class technology tech_variant submission.bioprojectsource.country earliest_date devstage_curation devstage_curation_coarse tissue_curation tissue_curation_coarse
15504 15504 ERR757154 ERX701784 ERS659467 ERP007147 PRJEB7420 Two Sets of Synthetic Spike in Controls for Size Selection and Data Normalisation in small RNA seq ena-STUDY-UNIVERSITY OF AMSTERDAM-01-10-2014-14:02:22:856-622 Other There is an increasing interest in complementing standard RNA seq experiments with small RNA expression data to obtain a comprehensive view of the transcriptome. Two aspects need to be considered when small RNA seq sRNA seq is used. First sRNA seq protocols are size selective and typically optimized for sequencing miRNAs. Second given the dynamic composition of the small RNA fraction a reliable normalization strategy is needed to identify differentially expressed sRNAs. To address both issues we here present two sets of synthetic RNA spike in controls for monitoring size selectivity and for performing normalization. Size spike ins comprising 18 oligoribonucleotides 10–300 nucleotides were designed and tested using controlled modifications to the size selectivity of sRNA seq. As an applied example the loss of RNA molecules <30 nucleotides during small RNA isolation from zebrafish oocytes is demonstrated. Normalization spike ins comprising 19 oligoribonucleotides were designed to use as an external reference for DESeq normalization. Spike in based normalization improved the quantification of predetermined fold changes. Lastly sRNA seq on female and male zebrafish demonstrated that the higher miRNA content in males was correctly preserved post spike in based normalization.       Normalization across length SAMEA3251321 UNIVERSITY OF AMSTERDAM ENA first public:2015 02 12|ENA last update:2015 02 12|External Id:SAMEA3251321|INSDC center alias:UNIVERSITY OF AMSTERDAM|INSDC center name:UNIVERSITY OF AMSTERDAM|INSDC first public:2015 02 12T17:06:49Z|INSDC last update:2015 02 12T09:52:20Z|INSDC status:public|Submitter Id:Female X|common name:zebrafish|dev stage:Adult|sample name:Female X|sex:female                 Ion Torrent Proton sequencing ena EXPERIMENT UNIVERSITY OF AMSTERDAM 12 02 2015 09:52:08:968 2 RID0035 1     miRNA-Seq TRANSCRIPTOMIC cDNA SINGLE ION_TORRENT Ion Torrent Proton   ERP007147 Ion Torrent Proton sequencing ENA FIRST PUBLIC:2015 02 12|ENA LAST UPDATE:2018 11 16 RID0035_003.fastq.gz fastq 275591336.0 7611054.0 ena RUN UNIVERSITY OF AMSTERDAM 12 02 2015 09:52:08:968 2 0:36.21 A:72212971;C:63331647;G:68054270;T:71992448;N:0 36       72212971 63331647 68054270 71992448 0 ERX701784 ERS659467 ERA410345 UNIVERSITY OF AMSTERDAM UNIVERSITY OF AMSTERDAM 1 0.76262   0.4273   0.8101   0.56676   8   B   usable mapping rate ion_torrent ion_torrent unknown small_rna unknown bulk unknown unknown   Netherlands 2015-02-12 Adult Adult Undetermined Undetermined
15505 15505 ERR757153 ERX701783 ERS659466 ERP007147 PRJEB7420 Two Sets of Synthetic Spike in Controls for Size Selection and Data Normalisation in small RNA seq ena-STUDY-UNIVERSITY OF AMSTERDAM-01-10-2014-14:02:22:856-622 Other There is an increasing interest in complementing standard RNA seq experiments with small RNA expression data to obtain a comprehensive view of the transcriptome. Two aspects need to be considered when small RNA seq sRNA seq is used. First sRNA seq protocols are size selective and typically optimized for sequencing miRNAs. Second given the dynamic composition of the small RNA fraction a reliable normalization strategy is needed to identify differentially expressed sRNAs. To address both issues we here present two sets of synthetic RNA spike in controls for monitoring size selectivity and for performing normalization. Size spike ins comprising 18 oligoribonucleotides 10–300 nucleotides were designed and tested using controlled modifications to the size selectivity of sRNA seq. As an applied example the loss of RNA molecules <30 nucleotides during small RNA isolation from zebrafish oocytes is demonstrated. Normalization spike ins comprising 19 oligoribonucleotides were designed to use as an external reference for DESeq normalization. Spike in based normalization improved the quantification of predetermined fold changes. Lastly sRNA seq on female and male zebrafish demonstrated that the higher miRNA content in males was correctly preserved post spike in based normalization.       Normalization across length SAMEA3251320 UNIVERSITY OF AMSTERDAM ENA first public:2015 02 12|ENA last update:2015 02 12|External Id:SAMEA3251320|INSDC center alias:UNIVERSITY OF AMSTERDAM|INSDC center name:UNIVERSITY OF AMSTERDAM|INSDC first public:2015 02 12T17:06:49Z|INSDC last update:2015 02 12T09:52:20Z|INSDC status:public|Submitter Id:Female 002|common name:zebrafish|dev stage:Adult|sample name:Female 002|sex:female                 Ion Torrent Proton sequencing ena EXPERIMENT UNIVERSITY OF AMSTERDAM 12 02 2015 09:52:08:968 1 RID0035 1     miRNA-Seq TRANSCRIPTOMIC cDNA SINGLE ION_TORRENT Ion Torrent Proton   ERP007147 Ion Torrent Proton sequencing ENA FIRST PUBLIC:2015 02 12|ENA LAST UPDATE:2018 11 16 RID0035_006.fastq.gz fastq 193706106.0 6343893.0 ena RUN UNIVERSITY OF AMSTERDAM 12 02 2015 09:52:08:968 1 0:30.53 A:52756888;C:42895374;G:46979869;T:51073975;N:0 30       52756888 42895374 46979869 51073975 0 ERX701783 ERS659466 ERA410345 UNIVERSITY OF AMSTERDAM UNIVERSITY OF AMSTERDAM 1 0.74991   0.41319   0.83002   0.55823   8   B   usable mapping rate ion_torrent ion_torrent unknown small_rna unknown bulk unknown unknown   Netherlands 2015-02-12 Adult Adult Undetermined Undetermined
15506 15506 ERR647638 ERX604074 ERS557914 ERP007147 PRJEB7420 Two Sets of Synthetic Spike in Controls for Size Selection and Data Normalisation in small RNA seq ena-STUDY-UNIVERSITY OF AMSTERDAM-01-10-2014-14:02:22:856-622 Other There is an increasing interest in complementing standard RNA seq experiments with small RNA expression data to obtain a comprehensive view of the transcriptome. Two aspects need to be considered when small RNA seq sRNA seq is used. First sRNA seq protocols are size selective and typically optimized for sequencing miRNAs. Second given the dynamic composition of the small RNA fraction a reliable normalization strategy is needed to identify differentially expressed sRNAs. To address both issues we here present two sets of synthetic RNA spike in controls for monitoring size selectivity and for performing normalization. Size spike ins comprising 18 oligoribonucleotides 10–300 nucleotides were designed and tested using controlled modifications to the size selectivity of sRNA seq. As an applied example the loss of RNA molecules <30 nucleotides during small RNA isolation from zebrafish oocytes is demonstrated. Normalization spike ins comprising 19 oligoribonucleotides were designed to use as an external reference for DESeq normalization. Spike in based normalization improved the quantification of predetermined fold changes. Lastly sRNA seq on female and male zebrafish demonstrated that the higher miRNA content in males was correctly preserved post spike in based normalization.       Male 001 SAMEA2796299 UNIVERSITY OF AMSTERDAM Alias:Male 001|ENA checklist:ERC000011|INSDC center alias:UNIVERSITY OF AMSTERDAM|INSDC center name:UNIVERSITY OF AMSTERDAM|INSDC first public:2014 11 29T17:02:28Z|INSDC last update:2014 10 01T14:42:44Z|INSDC status:public|SRA accession:ERS557914|Sample Name:ERS557914|Title:Male ZF|dev stage:Adult|sex:male|strain:ABxTL                 Ion Torrent Proton sequencing ena EXPERIMENT UNIVERSITY OF AMSTERDAM 01 10 2014 15:26:05:679 7 RID0015 Fold Change     miRNA-Seq TRANSCRIPTOMIC cDNA SINGLE ION_TORRENT Ion Torrent Proton   ERP007147 Ion Torrent Proton sequencing ENA FIRST PUBLIC:2014 11 29|ENA LAST UPDATE:2018 11 16 RID0015_007.fastq.gz fastq 181485343.0 7581675.0 ena RUN UNIVERSITY OF AMSTERDAM 01 10 2014 15:26:05:679 7 0:23.94 A:49408107;C:35660562;G:44520624;T:51896050;N:0 23       49408107 35660562 44520624 51896050 0 ERX604074 ERS557914 ERA363852 UNIVERSITY OF AMSTERDAM UNIVERSITY OF AMSTERDAM 1 0.75831   0.16017   0.90723   0.48684   13   B   usable mapping rate ion_torrent ion_torrent unknown small_rna unknown bulk unknown unknown   Netherlands 2014-10-01 Adult Adult Undetermined Undetermined
15507 15507 ERR647632 ERX604068 ERS557914 ERP007147 PRJEB7420 Two Sets of Synthetic Spike in Controls for Size Selection and Data Normalisation in small RNA seq ena-STUDY-UNIVERSITY OF AMSTERDAM-01-10-2014-14:02:22:856-622 Other There is an increasing interest in complementing standard RNA seq experiments with small RNA expression data to obtain a comprehensive view of the transcriptome. Two aspects need to be considered when small RNA seq sRNA seq is used. First sRNA seq protocols are size selective and typically optimized for sequencing miRNAs. Second given the dynamic composition of the small RNA fraction a reliable normalization strategy is needed to identify differentially expressed sRNAs. To address both issues we here present two sets of synthetic RNA spike in controls for monitoring size selectivity and for performing normalization. Size spike ins comprising 18 oligoribonucleotides 10–300 nucleotides were designed and tested using controlled modifications to the size selectivity of sRNA seq. As an applied example the loss of RNA molecules <30 nucleotides during small RNA isolation from zebrafish oocytes is demonstrated. Normalization spike ins comprising 19 oligoribonucleotides were designed to use as an external reference for DESeq normalization. Spike in based normalization improved the quantification of predetermined fold changes. Lastly sRNA seq on female and male zebrafish demonstrated that the higher miRNA content in males was correctly preserved post spike in based normalization.       Male 001 SAMEA2796299 UNIVERSITY OF AMSTERDAM Alias:Male 001|ENA checklist:ERC000011|INSDC center alias:UNIVERSITY OF AMSTERDAM|INSDC center name:UNIVERSITY OF AMSTERDAM|INSDC first public:2014 11 29T17:02:28Z|INSDC last update:2014 10 01T14:42:44Z|INSDC status:public|SRA accession:ERS557914|Sample Name:ERS557914|Title:Male ZF|dev stage:Adult|sex:male|strain:ABxTL                 Ion Torrent Proton sequencing ena EXPERIMENT UNIVERSITY OF AMSTERDAM 01 10 2014 15:26:05:678 1 RID0015 Fold Change     miRNA-Seq TRANSCRIPTOMIC cDNA SINGLE ION_TORRENT Ion Torrent Proton   ERP007147 Ion Torrent Proton sequencing ENA FIRST PUBLIC:2014 11 29|ENA LAST UPDATE:2018 11 16 RID0015_001.fastq.gz fastq 158714692.0 5407747.0 ena RUN UNIVERSITY OF AMSTERDAM 01 10 2014 15:26:05:678 1 0:29.35 A:42098161;C:32991434;G:38596553;T:45028544;N:0 29       42098161 32991434 38596553 45028544 0 ERX604068 ERS557914 ERA363852 UNIVERSITY OF AMSTERDAM UNIVERSITY OF AMSTERDAM 1 0.75901   0.20293   0.89505   0.48215   24   B   usable mapping rate ion_torrent ion_torrent unknown small_rna unknown bulk unknown unknown   Netherlands 2014-10-01 Adult Adult Undetermined Undetermined
15508 15508 ERR647636 ERX604072 ERS557914 ERP007147 PRJEB7420 Two Sets of Synthetic Spike in Controls for Size Selection and Data Normalisation in small RNA seq ena-STUDY-UNIVERSITY OF AMSTERDAM-01-10-2014-14:02:22:856-622 Other There is an increasing interest in complementing standard RNA seq experiments with small RNA expression data to obtain a comprehensive view of the transcriptome. Two aspects need to be considered when small RNA seq sRNA seq is used. First sRNA seq protocols are size selective and typically optimized for sequencing miRNAs. Second given the dynamic composition of the small RNA fraction a reliable normalization strategy is needed to identify differentially expressed sRNAs. To address both issues we here present two sets of synthetic RNA spike in controls for monitoring size selectivity and for performing normalization. Size spike ins comprising 18 oligoribonucleotides 10–300 nucleotides were designed and tested using controlled modifications to the size selectivity of sRNA seq. As an applied example the loss of RNA molecules <30 nucleotides during small RNA isolation from zebrafish oocytes is demonstrated. Normalization spike ins comprising 19 oligoribonucleotides were designed to use as an external reference for DESeq normalization. Spike in based normalization improved the quantification of predetermined fold changes. Lastly sRNA seq on female and male zebrafish demonstrated that the higher miRNA content in males was correctly preserved post spike in based normalization.       Male 001 SAMEA2796299 UNIVERSITY OF AMSTERDAM Alias:Male 001|ENA checklist:ERC000011|INSDC center alias:UNIVERSITY OF AMSTERDAM|INSDC center name:UNIVERSITY OF AMSTERDAM|INSDC first public:2014 11 29T17:02:28Z|INSDC last update:2014 10 01T14:42:44Z|INSDC status:public|SRA accession:ERS557914|Sample Name:ERS557914|Title:Male ZF|dev stage:Adult|sex:male|strain:ABxTL                 Ion Torrent Proton sequencing ena EXPERIMENT UNIVERSITY OF AMSTERDAM 01 10 2014 15:26:05:678 5 RID0015 Fold Change     miRNA-Seq TRANSCRIPTOMIC cDNA SINGLE ION_TORRENT Ion Torrent Proton   ERP007147 Ion Torrent Proton sequencing ENA FIRST PUBLIC:2014 11 29|ENA LAST UPDATE:2018 11 16 RID0015_005.fastq.gz fastq 124167412.0 4838491.0 ena RUN UNIVERSITY OF AMSTERDAM 01 10 2014 15:26:05:678 5 0:25.66 A:33439510;C:24728035;G:30897675;T:35102192;N:0 25       33439510 24728035 30897675 35102192 0 ERX604072 ERS557914 ERA363852 UNIVERSITY OF AMSTERDAM UNIVERSITY OF AMSTERDAM 1 0.76027   0.17783   0.89692   0.48421   30   B   usable mapping rate ion_torrent ion_torrent unknown small_rna unknown bulk unknown unknown   Netherlands 2014-10-01 Adult Adult Undetermined Undetermined
15509 15509 ERR647635 ERX604071 ERS557914 ERP007147 PRJEB7420 Two Sets of Synthetic Spike in Controls for Size Selection and Data Normalisation in small RNA seq ena-STUDY-UNIVERSITY OF AMSTERDAM-01-10-2014-14:02:22:856-622 Other There is an increasing interest in complementing standard RNA seq experiments with small RNA expression data to obtain a comprehensive view of the transcriptome. Two aspects need to be considered when small RNA seq sRNA seq is used. First sRNA seq protocols are size selective and typically optimized for sequencing miRNAs. Second given the dynamic composition of the small RNA fraction a reliable normalization strategy is needed to identify differentially expressed sRNAs. To address both issues we here present two sets of synthetic RNA spike in controls for monitoring size selectivity and for performing normalization. Size spike ins comprising 18 oligoribonucleotides 10–300 nucleotides were designed and tested using controlled modifications to the size selectivity of sRNA seq. As an applied example the loss of RNA molecules <30 nucleotides during small RNA isolation from zebrafish oocytes is demonstrated. Normalization spike ins comprising 19 oligoribonucleotides were designed to use as an external reference for DESeq normalization. Spike in based normalization improved the quantification of predetermined fold changes. Lastly sRNA seq on female and male zebrafish demonstrated that the higher miRNA content in males was correctly preserved post spike in based normalization.       Male 001 SAMEA2796299 UNIVERSITY OF AMSTERDAM Alias:Male 001|ENA checklist:ERC000011|INSDC center alias:UNIVERSITY OF AMSTERDAM|INSDC center name:UNIVERSITY OF AMSTERDAM|INSDC first public:2014 11 29T17:02:28Z|INSDC last update:2014 10 01T14:42:44Z|INSDC status:public|SRA accession:ERS557914|Sample Name:ERS557914|Title:Male ZF|dev stage:Adult|sex:male|strain:ABxTL                 Ion Torrent Proton sequencing ena EXPERIMENT UNIVERSITY OF AMSTERDAM 01 10 2014 15:26:05:678 4 RID0015 Fold Change     miRNA-Seq TRANSCRIPTOMIC cDNA SINGLE ION_TORRENT Ion Torrent Proton   ERP007147 Ion Torrent Proton sequencing ENA FIRST PUBLIC:2014 11 29|ENA LAST UPDATE:2018 11 16 RID0015_004.fastq.gz fastq 124786630.0 4701452.0 ena RUN UNIVERSITY OF AMSTERDAM 01 10 2014 15:26:05:678 4 0:26.54 A:33803418;C:24800330;G:30482560;T:35700322;N:0 26       33803418 24800330 30482560 35700322 0 ERX604071 ERS557914 ERA363852 UNIVERSITY OF AMSTERDAM UNIVERSITY OF AMSTERDAM 1 0.74872   0.18448   0.89789   0.50377   12   B   usable mapping rate ion_torrent ion_torrent unknown small_rna unknown bulk unknown unknown   Netherlands 2014-10-01 Adult Adult Undetermined Undetermined
15510 15510 ERR647634 ERX604070 ERS557914 ERP007147 PRJEB7420 Two Sets of Synthetic Spike in Controls for Size Selection and Data Normalisation in small RNA seq ena-STUDY-UNIVERSITY OF AMSTERDAM-01-10-2014-14:02:22:856-622 Other There is an increasing interest in complementing standard RNA seq experiments with small RNA expression data to obtain a comprehensive view of the transcriptome. Two aspects need to be considered when small RNA seq sRNA seq is used. First sRNA seq protocols are size selective and typically optimized for sequencing miRNAs. Second given the dynamic composition of the small RNA fraction a reliable normalization strategy is needed to identify differentially expressed sRNAs. To address both issues we here present two sets of synthetic RNA spike in controls for monitoring size selectivity and for performing normalization. Size spike ins comprising 18 oligoribonucleotides 10–300 nucleotides were designed and tested using controlled modifications to the size selectivity of sRNA seq. As an applied example the loss of RNA molecules <30 nucleotides during small RNA isolation from zebrafish oocytes is demonstrated. Normalization spike ins comprising 19 oligoribonucleotides were designed to use as an external reference for DESeq normalization. Spike in based normalization improved the quantification of predetermined fold changes. Lastly sRNA seq on female and male zebrafish demonstrated that the higher miRNA content in males was correctly preserved post spike in based normalization.       Male 001 SAMEA2796299 UNIVERSITY OF AMSTERDAM Alias:Male 001|ENA checklist:ERC000011|INSDC center alias:UNIVERSITY OF AMSTERDAM|INSDC center name:UNIVERSITY OF AMSTERDAM|INSDC first public:2014 11 29T17:02:28Z|INSDC last update:2014 10 01T14:42:44Z|INSDC status:public|SRA accession:ERS557914|Sample Name:ERS557914|Title:Male ZF|dev stage:Adult|sex:male|strain:ABxTL                 Ion Torrent Proton sequencing ena EXPERIMENT UNIVERSITY OF AMSTERDAM 01 10 2014 15:26:05:678 3 RID0015 Fold Change     miRNA-Seq TRANSCRIPTOMIC cDNA SINGLE ION_TORRENT Ion Torrent Proton   ERP007147 Ion Torrent Proton sequencing ENA FIRST PUBLIC:2014 11 29|ENA LAST UPDATE:2018 11 16 RID0015_003.fastq.gz fastq 110526752.0 4078127.0 ena RUN UNIVERSITY OF AMSTERDAM 01 10 2014 15:26:05:678 3 0:27.10 A:29453167;C:22981184;G:27444083;T:30648318;N:0 27       29453167 22981184 27444083 30648318 0 ERX604070 ERS557914 ERA363852 UNIVERSITY OF AMSTERDAM UNIVERSITY OF AMSTERDAM 1 0.73961   0.1804   0.89686   0.50069   22   B   usable mapping rate ion_torrent ion_torrent unknown small_rna unknown bulk unknown unknown   Netherlands 2014-10-01 Adult Adult Undetermined Undetermined
15511 15511 ERR647633 ERX604069 ERS557914 ERP007147 PRJEB7420 Two Sets of Synthetic Spike in Controls for Size Selection and Data Normalisation in small RNA seq ena-STUDY-UNIVERSITY OF AMSTERDAM-01-10-2014-14:02:22:856-622 Other There is an increasing interest in complementing standard RNA seq experiments with small RNA expression data to obtain a comprehensive view of the transcriptome. Two aspects need to be considered when small RNA seq sRNA seq is used. First sRNA seq protocols are size selective and typically optimized for sequencing miRNAs. Second given the dynamic composition of the small RNA fraction a reliable normalization strategy is needed to identify differentially expressed sRNAs. To address both issues we here present two sets of synthetic RNA spike in controls for monitoring size selectivity and for performing normalization. Size spike ins comprising 18 oligoribonucleotides 10–300 nucleotides were designed and tested using controlled modifications to the size selectivity of sRNA seq. As an applied example the loss of RNA molecules <30 nucleotides during small RNA isolation from zebrafish oocytes is demonstrated. Normalization spike ins comprising 19 oligoribonucleotides were designed to use as an external reference for DESeq normalization. Spike in based normalization improved the quantification of predetermined fold changes. Lastly sRNA seq on female and male zebrafish demonstrated that the higher miRNA content in males was correctly preserved post spike in based normalization.       Male 001 SAMEA2796299 UNIVERSITY OF AMSTERDAM Alias:Male 001|ENA checklist:ERC000011|INSDC center alias:UNIVERSITY OF AMSTERDAM|INSDC center name:UNIVERSITY OF AMSTERDAM|INSDC first public:2014 11 29T17:02:28Z|INSDC last update:2014 10 01T14:42:44Z|INSDC status:public|SRA accession:ERS557914|Sample Name:ERS557914|Title:Male ZF|dev stage:Adult|sex:male|strain:ABxTL                 Ion Torrent Proton sequencing ena EXPERIMENT UNIVERSITY OF AMSTERDAM 01 10 2014 15:26:05:678 2 RID0015 Fold Change     miRNA-Seq TRANSCRIPTOMIC cDNA SINGLE ION_TORRENT Ion Torrent Proton   ERP007147 Ion Torrent Proton sequencing ENA FIRST PUBLIC:2014 11 29|ENA LAST UPDATE:2018 11 16 RID0015_002.fastq.gz fastq 114721515.0 4468497.0 ena RUN UNIVERSITY OF AMSTERDAM 01 10 2014 15:26:05:678 2 0:25.67 A:31387085;C:22123581;G:28270438;T:32940411;N:0 25       31387085 22123581 28270438 32940411 0 ERX604069 ERS557914 ERA363852 UNIVERSITY OF AMSTERDAM UNIVERSITY OF AMSTERDAM 1 0.75767   0.17199   0.90118   0.49949   22   B   usable mapping rate ion_torrent ion_torrent unknown small_rna unknown bulk unknown unknown   Netherlands 2014-10-01 Adult Adult Undetermined Undetermined
15512 15512 ERR647645 ERX604081 ERS557914 ERP007147 PRJEB7420 Two Sets of Synthetic Spike in Controls for Size Selection and Data Normalisation in small RNA seq ena-STUDY-UNIVERSITY OF AMSTERDAM-01-10-2014-14:02:22:856-622 Other There is an increasing interest in complementing standard RNA seq experiments with small RNA expression data to obtain a comprehensive view of the transcriptome. Two aspects need to be considered when small RNA seq sRNA seq is used. First sRNA seq protocols are size selective and typically optimized for sequencing miRNAs. Second given the dynamic composition of the small RNA fraction a reliable normalization strategy is needed to identify differentially expressed sRNAs. To address both issues we here present two sets of synthetic RNA spike in controls for monitoring size selectivity and for performing normalization. Size spike ins comprising 18 oligoribonucleotides 10–300 nucleotides were designed and tested using controlled modifications to the size selectivity of sRNA seq. As an applied example the loss of RNA molecules <30 nucleotides during small RNA isolation from zebrafish oocytes is demonstrated. Normalization spike ins comprising 19 oligoribonucleotides were designed to use as an external reference for DESeq normalization. Spike in based normalization improved the quantification of predetermined fold changes. Lastly sRNA seq on female and male zebrafish demonstrated that the higher miRNA content in males was correctly preserved post spike in based normalization.       Male 001 SAMEA2796299 UNIVERSITY OF AMSTERDAM Alias:Male 001|ENA checklist:ERC000011|INSDC center alias:UNIVERSITY OF AMSTERDAM|INSDC center name:UNIVERSITY OF AMSTERDAM|INSDC first public:2014 11 29T17:02:28Z|INSDC last update:2014 10 01T14:42:44Z|INSDC status:public|SRA accession:ERS557914|Sample Name:ERS557914|Title:Male ZF|dev stage:Adult|sex:male|strain:ABxTL                 Ion Torrent Proton sequencing ena EXPERIMENT UNIVERSITY OF AMSTERDAM 01 10 2014 15:26:05:679 14 RID0015 Reproducibility     miRNA-Seq TRANSCRIPTOMIC cDNA SINGLE ION_TORRENT Ion Torrent Proton   ERP007147 Ion Torrent Proton sequencing ENA FIRST PUBLIC:2014 11 29|ENA LAST UPDATE:2018 11 16 RID0015_014.fastq.gz fastq 127742942.0 5487454.0 ena RUN UNIVERSITY OF AMSTERDAM 01 10 2014 15:26:05:679 14 0:23.28 A:35700746;C:25577569;G:31187339;T:35277288;N:0 23       35700746 25577569 31187339 35277288 0 ERX604081 ERS557914 ERA363852 UNIVERSITY OF AMSTERDAM UNIVERSITY OF AMSTERDAM 1 0.72451   0.1507   0.91078   0.47283   30   B   usable mapping rate ion_torrent ion_torrent unknown small_rna unknown bulk unknown unknown   Netherlands 2014-10-01 Adult Adult Undetermined Undetermined
15513 15513 ERR647644 ERX604080 ERS557914 ERP007147 PRJEB7420 Two Sets of Synthetic Spike in Controls for Size Selection and Data Normalisation in small RNA seq ena-STUDY-UNIVERSITY OF AMSTERDAM-01-10-2014-14:02:22:856-622 Other There is an increasing interest in complementing standard RNA seq experiments with small RNA expression data to obtain a comprehensive view of the transcriptome. Two aspects need to be considered when small RNA seq sRNA seq is used. First sRNA seq protocols are size selective and typically optimized for sequencing miRNAs. Second given the dynamic composition of the small RNA fraction a reliable normalization strategy is needed to identify differentially expressed sRNAs. To address both issues we here present two sets of synthetic RNA spike in controls for monitoring size selectivity and for performing normalization. Size spike ins comprising 18 oligoribonucleotides 10–300 nucleotides were designed and tested using controlled modifications to the size selectivity of sRNA seq. As an applied example the loss of RNA molecules <30 nucleotides during small RNA isolation from zebrafish oocytes is demonstrated. Normalization spike ins comprising 19 oligoribonucleotides were designed to use as an external reference for DESeq normalization. Spike in based normalization improved the quantification of predetermined fold changes. Lastly sRNA seq on female and male zebrafish demonstrated that the higher miRNA content in males was correctly preserved post spike in based normalization.       Male 001 SAMEA2796299 UNIVERSITY OF AMSTERDAM Alias:Male 001|ENA checklist:ERC000011|INSDC center alias:UNIVERSITY OF AMSTERDAM|INSDC center name:UNIVERSITY OF AMSTERDAM|INSDC first public:2014 11 29T17:02:28Z|INSDC last update:2014 10 01T14:42:44Z|INSDC status:public|SRA accession:ERS557914|Sample Name:ERS557914|Title:Male ZF|dev stage:Adult|sex:male|strain:ABxTL                 Ion Torrent Proton sequencing ena EXPERIMENT UNIVERSITY OF AMSTERDAM 01 10 2014 15:26:05:679 13 RID0015 Reproducibility     miRNA-Seq TRANSCRIPTOMIC cDNA SINGLE ION_TORRENT Ion Torrent Proton   ERP007147 Ion Torrent Proton sequencing ENA FIRST PUBLIC:2014 11 29|ENA LAST UPDATE:2018 11 16 RID0015_013.fastq.gz fastq 175319872.0 7193103.0 ena RUN UNIVERSITY OF AMSTERDAM 01 10 2014 15:26:05:679 13 0:24.37 A:48008107;C:34657005;G:43047489;T:49607271;N:0 24       48008107 34657005 43047489 49607271 0 ERX604080 ERS557914 ERA363852 UNIVERSITY OF AMSTERDAM UNIVERSITY OF AMSTERDAM 1 0.75539   0.16288   0.90388   0.47538   22   B   usable mapping rate ion_torrent ion_torrent unknown small_rna unknown bulk unknown unknown   Netherlands 2014-10-01 Adult Adult Undetermined Undetermined
15514 15514 ERR647643 ERX604079 ERS557914 ERP007147 PRJEB7420 Two Sets of Synthetic Spike in Controls for Size Selection and Data Normalisation in small RNA seq ena-STUDY-UNIVERSITY OF AMSTERDAM-01-10-2014-14:02:22:856-622 Other There is an increasing interest in complementing standard RNA seq experiments with small RNA expression data to obtain a comprehensive view of the transcriptome. Two aspects need to be considered when small RNA seq sRNA seq is used. First sRNA seq protocols are size selective and typically optimized for sequencing miRNAs. Second given the dynamic composition of the small RNA fraction a reliable normalization strategy is needed to identify differentially expressed sRNAs. To address both issues we here present two sets of synthetic RNA spike in controls for monitoring size selectivity and for performing normalization. Size spike ins comprising 18 oligoribonucleotides 10–300 nucleotides were designed and tested using controlled modifications to the size selectivity of sRNA seq. As an applied example the loss of RNA molecules <30 nucleotides during small RNA isolation from zebrafish oocytes is demonstrated. Normalization spike ins comprising 19 oligoribonucleotides were designed to use as an external reference for DESeq normalization. Spike in based normalization improved the quantification of predetermined fold changes. Lastly sRNA seq on female and male zebrafish demonstrated that the higher miRNA content in males was correctly preserved post spike in based normalization.       Male 001 SAMEA2796299 UNIVERSITY OF AMSTERDAM Alias:Male 001|ENA checklist:ERC000011|INSDC center alias:UNIVERSITY OF AMSTERDAM|INSDC center name:UNIVERSITY OF AMSTERDAM|INSDC first public:2014 11 29T17:02:28Z|INSDC last update:2014 10 01T14:42:44Z|INSDC status:public|SRA accession:ERS557914|Sample Name:ERS557914|Title:Male ZF|dev stage:Adult|sex:male|strain:ABxTL                 Ion Torrent Proton sequencing ena EXPERIMENT UNIVERSITY OF AMSTERDAM 01 10 2014 15:26:05:679 12 RID0015 Reproducibility     miRNA-Seq TRANSCRIPTOMIC cDNA SINGLE ION_TORRENT Ion Torrent Proton   ERP007147 Ion Torrent Proton sequencing ENA FIRST PUBLIC:2014 11 29|ENA LAST UPDATE:2018 11 16 RID0015_012.fastq.gz fastq 177443752.0 7567733.0 ena RUN UNIVERSITY OF AMSTERDAM 01 10 2014 15:26:05:679 12 0:23.45 A:48143286;C:36300870;G:44060167;T:48939429;N:0 23       48143286 36300870 44060167 48939429 0 ERX604079 ERS557914 ERA363852 UNIVERSITY OF AMSTERDAM UNIVERSITY OF AMSTERDAM 1 0.74839   0.15018   0.90782   0.49076   27   B   usable mapping rate ion_torrent ion_torrent unknown small_rna unknown bulk unknown unknown   Netherlands 2014-10-01 Adult Adult Undetermined Undetermined
15515 15515 ERR647642 ERX604078 ERS557914 ERP007147 PRJEB7420 Two Sets of Synthetic Spike in Controls for Size Selection and Data Normalisation in small RNA seq ena-STUDY-UNIVERSITY OF AMSTERDAM-01-10-2014-14:02:22:856-622 Other There is an increasing interest in complementing standard RNA seq experiments with small RNA expression data to obtain a comprehensive view of the transcriptome. Two aspects need to be considered when small RNA seq sRNA seq is used. First sRNA seq protocols are size selective and typically optimized for sequencing miRNAs. Second given the dynamic composition of the small RNA fraction a reliable normalization strategy is needed to identify differentially expressed sRNAs. To address both issues we here present two sets of synthetic RNA spike in controls for monitoring size selectivity and for performing normalization. Size spike ins comprising 18 oligoribonucleotides 10–300 nucleotides were designed and tested using controlled modifications to the size selectivity of sRNA seq. As an applied example the loss of RNA molecules <30 nucleotides during small RNA isolation from zebrafish oocytes is demonstrated. Normalization spike ins comprising 19 oligoribonucleotides were designed to use as an external reference for DESeq normalization. Spike in based normalization improved the quantification of predetermined fold changes. Lastly sRNA seq on female and male zebrafish demonstrated that the higher miRNA content in males was correctly preserved post spike in based normalization.       Male 001 SAMEA2796299 UNIVERSITY OF AMSTERDAM Alias:Male 001|ENA checklist:ERC000011|INSDC center alias:UNIVERSITY OF AMSTERDAM|INSDC center name:UNIVERSITY OF AMSTERDAM|INSDC first public:2014 11 29T17:02:28Z|INSDC last update:2014 10 01T14:42:44Z|INSDC status:public|SRA accession:ERS557914|Sample Name:ERS557914|Title:Male ZF|dev stage:Adult|sex:male|strain:ABxTL                 Ion Torrent Proton sequencing ena EXPERIMENT UNIVERSITY OF AMSTERDAM 01 10 2014 15:26:05:679 11 RID0015 Reproducibility     miRNA-Seq TRANSCRIPTOMIC cDNA SINGLE ION_TORRENT Ion Torrent Proton   ERP007147 Ion Torrent Proton sequencing ENA FIRST PUBLIC:2014 11 29|ENA LAST UPDATE:2018 11 16 RID0015_011.fastq.gz fastq 163389373.0 6320373.0 ena RUN UNIVERSITY OF AMSTERDAM 01 10 2014 15:26:05:679 11 0:25.85 A:44540588;C:32608476;G:40066295;T:46174014;N:0 25       44540588 32608476 40066295 46174014 0 ERX604078 ERS557914 ERA363852 UNIVERSITY OF AMSTERDAM UNIVERSITY OF AMSTERDAM 1 0.75948   0.17375   0.90252   0.47536   33   B   usable mapping rate ion_torrent ion_torrent unknown small_rna unknown bulk unknown unknown   Netherlands 2014-10-01 Adult Adult Undetermined Undetermined
15516 15516 ERR647641 ERX604077 ERS557914 ERP007147 PRJEB7420 Two Sets of Synthetic Spike in Controls for Size Selection and Data Normalisation in small RNA seq ena-STUDY-UNIVERSITY OF AMSTERDAM-01-10-2014-14:02:22:856-622 Other There is an increasing interest in complementing standard RNA seq experiments with small RNA expression data to obtain a comprehensive view of the transcriptome. Two aspects need to be considered when small RNA seq sRNA seq is used. First sRNA seq protocols are size selective and typically optimized for sequencing miRNAs. Second given the dynamic composition of the small RNA fraction a reliable normalization strategy is needed to identify differentially expressed sRNAs. To address both issues we here present two sets of synthetic RNA spike in controls for monitoring size selectivity and for performing normalization. Size spike ins comprising 18 oligoribonucleotides 10–300 nucleotides were designed and tested using controlled modifications to the size selectivity of sRNA seq. As an applied example the loss of RNA molecules <30 nucleotides during small RNA isolation from zebrafish oocytes is demonstrated. Normalization spike ins comprising 19 oligoribonucleotides were designed to use as an external reference for DESeq normalization. Spike in based normalization improved the quantification of predetermined fold changes. Lastly sRNA seq on female and male zebrafish demonstrated that the higher miRNA content in males was correctly preserved post spike in based normalization.       Male 001 SAMEA2796299 UNIVERSITY OF AMSTERDAM Alias:Male 001|ENA checklist:ERC000011|INSDC center alias:UNIVERSITY OF AMSTERDAM|INSDC center name:UNIVERSITY OF AMSTERDAM|INSDC first public:2014 11 29T17:02:28Z|INSDC last update:2014 10 01T14:42:44Z|INSDC status:public|SRA accession:ERS557914|Sample Name:ERS557914|Title:Male ZF|dev stage:Adult|sex:male|strain:ABxTL                 Ion Torrent Proton sequencing ena EXPERIMENT UNIVERSITY OF AMSTERDAM 01 10 2014 15:26:05:679 10 RID0015 Reproducibility     miRNA-Seq TRANSCRIPTOMIC cDNA SINGLE ION_TORRENT Ion Torrent Proton   ERP007147 Ion Torrent Proton sequencing ENA FIRST PUBLIC:2014 11 29|ENA LAST UPDATE:2018 11 16 RID0015_010.fastq.gz fastq 128059692.0 5701206.0 ena RUN UNIVERSITY OF AMSTERDAM 01 10 2014 15:26:05:679 10 0:22.46 A:35418188;C:24802528;G:31593786;T:36245190;N:0 22       35418188 24802528 31593786 36245190 0 ERX604077 ERS557914 ERA363852 UNIVERSITY OF AMSTERDAM UNIVERSITY OF AMSTERDAM 1 0.72586   0.1331   0.92514   0.4216   22   B   usable mapping rate ion_torrent ion_torrent unknown small_rna unknown bulk unknown unknown   Netherlands 2014-10-01 Adult Adult Undetermined Undetermined
15517 15517 ERR647637 ERX604073 ERS557914 ERP007147 PRJEB7420 Two Sets of Synthetic Spike in Controls for Size Selection and Data Normalisation in small RNA seq ena-STUDY-UNIVERSITY OF AMSTERDAM-01-10-2014-14:02:22:856-622 Other There is an increasing interest in complementing standard RNA seq experiments with small RNA expression data to obtain a comprehensive view of the transcriptome. Two aspects need to be considered when small RNA seq sRNA seq is used. First sRNA seq protocols are size selective and typically optimized for sequencing miRNAs. Second given the dynamic composition of the small RNA fraction a reliable normalization strategy is needed to identify differentially expressed sRNAs. To address both issues we here present two sets of synthetic RNA spike in controls for monitoring size selectivity and for performing normalization. Size spike ins comprising 18 oligoribonucleotides 10–300 nucleotides were designed and tested using controlled modifications to the size selectivity of sRNA seq. As an applied example the loss of RNA molecules <30 nucleotides during small RNA isolation from zebrafish oocytes is demonstrated. Normalization spike ins comprising 19 oligoribonucleotides were designed to use as an external reference for DESeq normalization. Spike in based normalization improved the quantification of predetermined fold changes. Lastly sRNA seq on female and male zebrafish demonstrated that the higher miRNA content in males was correctly preserved post spike in based normalization.       Male 001 SAMEA2796299 UNIVERSITY OF AMSTERDAM Alias:Male 001|ENA checklist:ERC000011|INSDC center alias:UNIVERSITY OF AMSTERDAM|INSDC center name:UNIVERSITY OF AMSTERDAM|INSDC first public:2014 11 29T17:02:28Z|INSDC last update:2014 10 01T14:42:44Z|INSDC status:public|SRA accession:ERS557914|Sample Name:ERS557914|Title:Male ZF|dev stage:Adult|sex:male|strain:ABxTL                 Ion Torrent Proton sequencing ena EXPERIMENT UNIVERSITY OF AMSTERDAM 01 10 2014 15:26:05:678 6 RID0015 Fold Change     miRNA-Seq TRANSCRIPTOMIC cDNA SINGLE ION_TORRENT Ion Torrent Proton   ERP007147 Ion Torrent Proton sequencing ENA FIRST PUBLIC:2014 11 29|ENA LAST UPDATE:2018 11 16 RID0015_006.fastq.gz fastq 165327404.0 6835740.0 ena RUN UNIVERSITY OF AMSTERDAM 01 10 2014 15:26:05:679 6 0:24.19 A:45252916;C:32442683;G:40306020;T:47325785;N:0 24       45252916 32442683 40306020 47325785 0 ERX604073 ERS557914 ERA363852 UNIVERSITY OF AMSTERDAM UNIVERSITY OF AMSTERDAM 1 0.74944   0.16   0.91068   0.47704   21   B   usable mapping rate ion_torrent ion_torrent unknown small_rna unknown bulk unknown unknown   Netherlands 2014-10-01 Adult Adult Undetermined Undetermined
15518 15518 ERR647640 ERX604076 ERS557914 ERP007147 PRJEB7420 Two Sets of Synthetic Spike in Controls for Size Selection and Data Normalisation in small RNA seq ena-STUDY-UNIVERSITY OF AMSTERDAM-01-10-2014-14:02:22:856-622 Other There is an increasing interest in complementing standard RNA seq experiments with small RNA expression data to obtain a comprehensive view of the transcriptome. Two aspects need to be considered when small RNA seq sRNA seq is used. First sRNA seq protocols are size selective and typically optimized for sequencing miRNAs. Second given the dynamic composition of the small RNA fraction a reliable normalization strategy is needed to identify differentially expressed sRNAs. To address both issues we here present two sets of synthetic RNA spike in controls for monitoring size selectivity and for performing normalization. Size spike ins comprising 18 oligoribonucleotides 10–300 nucleotides were designed and tested using controlled modifications to the size selectivity of sRNA seq. As an applied example the loss of RNA molecules <30 nucleotides during small RNA isolation from zebrafish oocytes is demonstrated. Normalization spike ins comprising 19 oligoribonucleotides were designed to use as an external reference for DESeq normalization. Spike in based normalization improved the quantification of predetermined fold changes. Lastly sRNA seq on female and male zebrafish demonstrated that the higher miRNA content in males was correctly preserved post spike in based normalization.       Male 001 SAMEA2796299 UNIVERSITY OF AMSTERDAM Alias:Male 001|ENA checklist:ERC000011|INSDC center alias:UNIVERSITY OF AMSTERDAM|INSDC center name:UNIVERSITY OF AMSTERDAM|INSDC first public:2014 11 29T17:02:28Z|INSDC last update:2014 10 01T14:42:44Z|INSDC status:public|SRA accession:ERS557914|Sample Name:ERS557914|Title:Male ZF|dev stage:Adult|sex:male|strain:ABxTL                 Ion Torrent Proton sequencing ena EXPERIMENT UNIVERSITY OF AMSTERDAM 01 10 2014 15:26:05:679 9 RID0015 Reproducibility     miRNA-Seq TRANSCRIPTOMIC cDNA SINGLE ION_TORRENT Ion Torrent Proton   ERP007147 Ion Torrent Proton sequencing ENA FIRST PUBLIC:2014 11 29|ENA LAST UPDATE:2018 11 16 RID0015_009.fastq.gz fastq 134426587.0 5846482.0 ena RUN UNIVERSITY OF AMSTERDAM 01 10 2014 15:26:05:679 9 0:22.99 A:36803811;C:26283055;G:33442229;T:37897492;N:0 22       36803811 26283055 33442229 37897492 0 ERX604076 ERS557914 ERA363852 UNIVERSITY OF AMSTERDAM UNIVERSITY OF AMSTERDAM 1 0.74219   0.13958   0.91837   0.47819   22   B   usable mapping rate ion_torrent ion_torrent unknown small_rna unknown bulk unknown unknown   Netherlands 2014-10-01 Adult Adult Undetermined Undetermined
15519 15519 ERR647639 ERX604075 ERS557914 ERP007147 PRJEB7420 Two Sets of Synthetic Spike in Controls for Size Selection and Data Normalisation in small RNA seq ena-STUDY-UNIVERSITY OF AMSTERDAM-01-10-2014-14:02:22:856-622 Other There is an increasing interest in complementing standard RNA seq experiments with small RNA expression data to obtain a comprehensive view of the transcriptome. Two aspects need to be considered when small RNA seq sRNA seq is used. First sRNA seq protocols are size selective and typically optimized for sequencing miRNAs. Second given the dynamic composition of the small RNA fraction a reliable normalization strategy is needed to identify differentially expressed sRNAs. To address both issues we here present two sets of synthetic RNA spike in controls for monitoring size selectivity and for performing normalization. Size spike ins comprising 18 oligoribonucleotides 10–300 nucleotides were designed and tested using controlled modifications to the size selectivity of sRNA seq. As an applied example the loss of RNA molecules <30 nucleotides during small RNA isolation from zebrafish oocytes is demonstrated. Normalization spike ins comprising 19 oligoribonucleotides were designed to use as an external reference for DESeq normalization. Spike in based normalization improved the quantification of predetermined fold changes. Lastly sRNA seq on female and male zebrafish demonstrated that the higher miRNA content in males was correctly preserved post spike in based normalization.       Male 001 SAMEA2796299 UNIVERSITY OF AMSTERDAM Alias:Male 001|ENA checklist:ERC000011|INSDC center alias:UNIVERSITY OF AMSTERDAM|INSDC center name:UNIVERSITY OF AMSTERDAM|INSDC first public:2014 11 29T17:02:28Z|INSDC last update:2014 10 01T14:42:44Z|INSDC status:public|SRA accession:ERS557914|Sample Name:ERS557914|Title:Male ZF|dev stage:Adult|sex:male|strain:ABxTL                 Ion Torrent Proton sequencing ena EXPERIMENT UNIVERSITY OF AMSTERDAM 01 10 2014 15:26:05:679 8 RID0015 Fold Change     miRNA-Seq TRANSCRIPTOMIC cDNA SINGLE ION_TORRENT Ion Torrent Proton   ERP007147 Ion Torrent Proton sequencing ENA FIRST PUBLIC:2014 11 29|ENA LAST UPDATE:2018 11 16 RID0015_008.fastq.gz fastq 164369360.0 6762174.0 ena RUN UNIVERSITY OF AMSTERDAM 01 10 2014 15:26:05:679 8 0:24.31 A:44826982;C:33030972;G:40615427;T:45895979;N:0 24       44826982 33030972 40615427 45895979 0 ERX604075 ERS557914 ERA363852 UNIVERSITY OF AMSTERDAM UNIVERSITY OF AMSTERDAM 1 0.75513   0.16254   0.90534   0.50535   22   B   usable mapping rate ion_torrent ion_torrent unknown small_rna unknown bulk unknown unknown   Netherlands 2014-10-01 Adult Adult Undetermined Undetermined
15520 15520 ERR647623 ERX604059 ERS557914 ERP007147 PRJEB7420 Two Sets of Synthetic Spike in Controls for Size Selection and Data Normalisation in small RNA seq ena-STUDY-UNIVERSITY OF AMSTERDAM-01-10-2014-14:02:22:856-622 Other There is an increasing interest in complementing standard RNA seq experiments with small RNA expression data to obtain a comprehensive view of the transcriptome. Two aspects need to be considered when small RNA seq sRNA seq is used. First sRNA seq protocols are size selective and typically optimized for sequencing miRNAs. Second given the dynamic composition of the small RNA fraction a reliable normalization strategy is needed to identify differentially expressed sRNAs. To address both issues we here present two sets of synthetic RNA spike in controls for monitoring size selectivity and for performing normalization. Size spike ins comprising 18 oligoribonucleotides 10–300 nucleotides were designed and tested using controlled modifications to the size selectivity of sRNA seq. As an applied example the loss of RNA molecules <30 nucleotides during small RNA isolation from zebrafish oocytes is demonstrated. Normalization spike ins comprising 19 oligoribonucleotides were designed to use as an external reference for DESeq normalization. Spike in based normalization improved the quantification of predetermined fold changes. Lastly sRNA seq on female and male zebrafish demonstrated that the higher miRNA content in males was correctly preserved post spike in based normalization.       Male 001 SAMEA2796299 UNIVERSITY OF AMSTERDAM Alias:Male 001|ENA checklist:ERC000011|INSDC center alias:UNIVERSITY OF AMSTERDAM|INSDC center name:UNIVERSITY OF AMSTERDAM|INSDC first public:2014 11 29T17:02:28Z|INSDC last update:2014 10 01T14:42:44Z|INSDC status:public|SRA accession:ERS557914|Sample Name:ERS557914|Title:Male ZF|dev stage:Adult|sex:male|strain:ABxTL                 Ion Torrent Proton sequencing ena EXPERIMENT UNIVERSITY OF AMSTERDAM 01 10 2014 15:08:22:087 8 RID0017 EtOH conc. experiment     miRNA-Seq TRANSCRIPTOMIC cDNA SINGLE ION_TORRENT Ion Torrent Proton   ERP007147 Ion Torrent Proton sequencing ENA FIRST PUBLIC:2014 11 29|ENA LAST UPDATE:2018 11 16 RID0017_008.fastq.gz fastq 198146766.0 7373392.0 ena RUN UNIVERSITY OF AMSTERDAM 01 10 2014 15:08:22:087 8 0:26.87 A:50518864;C:42346985;G:50683068;T:54597849;N:0 26       50518864 42346985 50683068 54597849 0 ERX604059 ERS557914 ERA363851 UNIVERSITY OF AMSTERDAM UNIVERSITY OF AMSTERDAM 1 0.73719   0.16567   0.89869   0.49695   76   B   usable mapping rate ion_torrent ion_torrent unknown small_rna unknown bulk unknown unknown   Netherlands 2014-10-01 Adult Adult Undetermined Undetermined
15521 15521 ERR647622 ERX604058 ERS557914 ERP007147 PRJEB7420 Two Sets of Synthetic Spike in Controls for Size Selection and Data Normalisation in small RNA seq ena-STUDY-UNIVERSITY OF AMSTERDAM-01-10-2014-14:02:22:856-622 Other There is an increasing interest in complementing standard RNA seq experiments with small RNA expression data to obtain a comprehensive view of the transcriptome. Two aspects need to be considered when small RNA seq sRNA seq is used. First sRNA seq protocols are size selective and typically optimized for sequencing miRNAs. Second given the dynamic composition of the small RNA fraction a reliable normalization strategy is needed to identify differentially expressed sRNAs. To address both issues we here present two sets of synthetic RNA spike in controls for monitoring size selectivity and for performing normalization. Size spike ins comprising 18 oligoribonucleotides 10–300 nucleotides were designed and tested using controlled modifications to the size selectivity of sRNA seq. As an applied example the loss of RNA molecules <30 nucleotides during small RNA isolation from zebrafish oocytes is demonstrated. Normalization spike ins comprising 19 oligoribonucleotides were designed to use as an external reference for DESeq normalization. Spike in based normalization improved the quantification of predetermined fold changes. Lastly sRNA seq on female and male zebrafish demonstrated that the higher miRNA content in males was correctly preserved post spike in based normalization.       Male 001 SAMEA2796299 UNIVERSITY OF AMSTERDAM Alias:Male 001|ENA checklist:ERC000011|INSDC center alias:UNIVERSITY OF AMSTERDAM|INSDC center name:UNIVERSITY OF AMSTERDAM|INSDC first public:2014 11 29T17:02:28Z|INSDC last update:2014 10 01T14:42:44Z|INSDC status:public|SRA accession:ERS557914|Sample Name:ERS557914|Title:Male ZF|dev stage:Adult|sex:male|strain:ABxTL                 Ion Torrent Proton sequencing ena EXPERIMENT UNIVERSITY OF AMSTERDAM 01 10 2014 15:08:22:087 7 RID0017 EtOH conc. experiment     miRNA-Seq TRANSCRIPTOMIC cDNA SINGLE ION_TORRENT Ion Torrent Proton   ERP007147 Ion Torrent Proton sequencing ENA FIRST PUBLIC:2014 11 29|ENA LAST UPDATE:2018 11 16 RID0017_007.fastq.gz fastq 240260456.0 9194064.0 ena RUN UNIVERSITY OF AMSTERDAM 01 10 2014 15:08:22:087 7 0:26.13 A:62434032;C:50861577;G:60916218;T:66048629;N:0 26       62434032 50861577 60916218 66048629 0 ERX604058 ERS557914 ERA363851 UNIVERSITY OF AMSTERDAM UNIVERSITY OF AMSTERDAM 1 0.73042   0.17017   0.89509   0.51784   44   B   usable mapping rate ion_torrent ion_torrent unknown small_rna unknown bulk unknown unknown   Netherlands 2014-10-01 Adult Adult Undetermined Undetermined
15522 15522 ERR647620 ERX604056 ERS557914 ERP007147 PRJEB7420 Two Sets of Synthetic Spike in Controls for Size Selection and Data Normalisation in small RNA seq ena-STUDY-UNIVERSITY OF AMSTERDAM-01-10-2014-14:02:22:856-622 Other There is an increasing interest in complementing standard RNA seq experiments with small RNA expression data to obtain a comprehensive view of the transcriptome. Two aspects need to be considered when small RNA seq sRNA seq is used. First sRNA seq protocols are size selective and typically optimized for sequencing miRNAs. Second given the dynamic composition of the small RNA fraction a reliable normalization strategy is needed to identify differentially expressed sRNAs. To address both issues we here present two sets of synthetic RNA spike in controls for monitoring size selectivity and for performing normalization. Size spike ins comprising 18 oligoribonucleotides 10–300 nucleotides were designed and tested using controlled modifications to the size selectivity of sRNA seq. As an applied example the loss of RNA molecules <30 nucleotides during small RNA isolation from zebrafish oocytes is demonstrated. Normalization spike ins comprising 19 oligoribonucleotides were designed to use as an external reference for DESeq normalization. Spike in based normalization improved the quantification of predetermined fold changes. Lastly sRNA seq on female and male zebrafish demonstrated that the higher miRNA content in males was correctly preserved post spike in based normalization.       Male 001 SAMEA2796299 UNIVERSITY OF AMSTERDAM Alias:Male 001|ENA checklist:ERC000011|INSDC center alias:UNIVERSITY OF AMSTERDAM|INSDC center name:UNIVERSITY OF AMSTERDAM|INSDC first public:2014 11 29T17:02:28Z|INSDC last update:2014 10 01T14:42:44Z|INSDC status:public|SRA accession:ERS557914|Sample Name:ERS557914|Title:Male ZF|dev stage:Adult|sex:male|strain:ABxTL                 Ion Torrent Proton sequencing ena EXPERIMENT UNIVERSITY OF AMSTERDAM 01 10 2014 15:08:22:087 5 RID0017 EtOH conc. experiment     miRNA-Seq TRANSCRIPTOMIC cDNA SINGLE ION_TORRENT Ion Torrent Proton   ERP007147 Ion Torrent Proton sequencing ENA FIRST PUBLIC:2014 11 29|ENA LAST UPDATE:2018 11 16 RID0017_005.fastq.gz fastq 140470735.0 4903805.0 ena RUN UNIVERSITY OF AMSTERDAM 01 10 2014 15:08:22:087 5 0:28.65 A:35312706;C:29191443;G:36788541;T:39178045;N:0 28       35312706 29191443 36788541 39178045 0 ERX604056 ERS557914 ERA363851 UNIVERSITY OF AMSTERDAM UNIVERSITY OF AMSTERDAM 1 0.76081   0.16609   0.90216   0.48155   72   B   usable mapping rate ion_torrent ion_torrent unknown small_rna unknown bulk unknown unknown   Netherlands 2014-10-01 Adult Adult Undetermined Undetermined
15523 15523 ERR647617 ERX604053 ERS557914 ERP007147 PRJEB7420 Two Sets of Synthetic Spike in Controls for Size Selection and Data Normalisation in small RNA seq ena-STUDY-UNIVERSITY OF AMSTERDAM-01-10-2014-14:02:22:856-622 Other There is an increasing interest in complementing standard RNA seq experiments with small RNA expression data to obtain a comprehensive view of the transcriptome. Two aspects need to be considered when small RNA seq sRNA seq is used. First sRNA seq protocols are size selective and typically optimized for sequencing miRNAs. Second given the dynamic composition of the small RNA fraction a reliable normalization strategy is needed to identify differentially expressed sRNAs. To address both issues we here present two sets of synthetic RNA spike in controls for monitoring size selectivity and for performing normalization. Size spike ins comprising 18 oligoribonucleotides 10–300 nucleotides were designed and tested using controlled modifications to the size selectivity of sRNA seq. As an applied example the loss of RNA molecules <30 nucleotides during small RNA isolation from zebrafish oocytes is demonstrated. Normalization spike ins comprising 19 oligoribonucleotides were designed to use as an external reference for DESeq normalization. Spike in based normalization improved the quantification of predetermined fold changes. Lastly sRNA seq on female and male zebrafish demonstrated that the higher miRNA content in males was correctly preserved post spike in based normalization.       Male 001 SAMEA2796299 UNIVERSITY OF AMSTERDAM Alias:Male 001|ENA checklist:ERC000011|INSDC center alias:UNIVERSITY OF AMSTERDAM|INSDC center name:UNIVERSITY OF AMSTERDAM|INSDC first public:2014 11 29T17:02:28Z|INSDC last update:2014 10 01T14:42:44Z|INSDC status:public|SRA accession:ERS557914|Sample Name:ERS557914|Title:Male ZF|dev stage:Adult|sex:male|strain:ABxTL                 Ion Torrent Proton sequencing ena EXPERIMENT UNIVERSITY OF AMSTERDAM 01 10 2014 15:08:22:087 2 RID0017 EtOH conc. experiment     miRNA-Seq TRANSCRIPTOMIC cDNA SINGLE ION_TORRENT Ion Torrent Proton   ERP007147 Ion Torrent Proton sequencing ENA FIRST PUBLIC:2014 11 29|ENA LAST UPDATE:2018 11 16 RID0017_002.fastq.gz fastq 113953599.0 4875815.0 ena RUN UNIVERSITY OF AMSTERDAM 01 10 2014 15:08:22:087 2 0:23.37 A:29576305;C:23096908;G:29656780;T:31623606;N:0 23       29576305 23096908 29656780 31623606 0 ERX604053 ERS557914 ERA363851 UNIVERSITY OF AMSTERDAM UNIVERSITY OF AMSTERDAM 1 0.70891   0.13528   0.91208   0.4622   24   B   usable mapping rate ion_torrent ion_torrent unknown small_rna unknown bulk unknown unknown   Netherlands 2014-10-01 Adult Adult Undetermined Undetermined
15524 15524 ERR647624 ERX604060 ERS557914 ERP007147 PRJEB7420 Two Sets of Synthetic Spike in Controls for Size Selection and Data Normalisation in small RNA seq ena-STUDY-UNIVERSITY OF AMSTERDAM-01-10-2014-14:02:22:856-622 Other There is an increasing interest in complementing standard RNA seq experiments with small RNA expression data to obtain a comprehensive view of the transcriptome. Two aspects need to be considered when small RNA seq sRNA seq is used. First sRNA seq protocols are size selective and typically optimized for sequencing miRNAs. Second given the dynamic composition of the small RNA fraction a reliable normalization strategy is needed to identify differentially expressed sRNAs. To address both issues we here present two sets of synthetic RNA spike in controls for monitoring size selectivity and for performing normalization. Size spike ins comprising 18 oligoribonucleotides 10–300 nucleotides were designed and tested using controlled modifications to the size selectivity of sRNA seq. As an applied example the loss of RNA molecules <30 nucleotides during small RNA isolation from zebrafish oocytes is demonstrated. Normalization spike ins comprising 19 oligoribonucleotides were designed to use as an external reference for DESeq normalization. Spike in based normalization improved the quantification of predetermined fold changes. Lastly sRNA seq on female and male zebrafish demonstrated that the higher miRNA content in males was correctly preserved post spike in based normalization.       Male 001 SAMEA2796299 UNIVERSITY OF AMSTERDAM Alias:Male 001|ENA checklist:ERC000011|INSDC center alias:UNIVERSITY OF AMSTERDAM|INSDC center name:UNIVERSITY OF AMSTERDAM|INSDC first public:2014 11 29T17:02:28Z|INSDC last update:2014 10 01T14:42:44Z|INSDC status:public|SRA accession:ERS557914|Sample Name:ERS557914|Title:Male ZF|dev stage:Adult|sex:male|strain:ABxTL                 Ion Torrent Proton sequencing ena EXPERIMENT UNIVERSITY OF AMSTERDAM 01 10 2014 15:08:22:087 9 RID0017 EtOH conc. experiment     miRNA-Seq TRANSCRIPTOMIC cDNA SINGLE ION_TORRENT Ion Torrent Proton   ERP007147 Ion Torrent Proton sequencing ENA FIRST PUBLIC:2014 11 29|ENA LAST UPDATE:2018 11 16 RID0017_009.fastq.gz fastq 127769435.0 5492615.0 ena RUN UNIVERSITY OF AMSTERDAM 01 10 2014 15:08:22:087 9 0:23.26 A:34397217;C:25332113;G:31991788;T:36048317;N:0 23       34397217 25332113 31991788 36048317 0 ERX604060 ERS557914 ERA363851 UNIVERSITY OF AMSTERDAM UNIVERSITY OF AMSTERDAM 1 0.71147   0.1472   0.91015   0.49014   22   B   usable mapping rate ion_torrent ion_torrent unknown small_rna unknown bulk unknown unknown   Netherlands 2014-10-01 Adult Adult Undetermined Undetermined
15525 15525 ERR647629 ERX604065 ERS557914 ERP007147 PRJEB7420 Two Sets of Synthetic Spike in Controls for Size Selection and Data Normalisation in small RNA seq ena-STUDY-UNIVERSITY OF AMSTERDAM-01-10-2014-14:02:22:856-622 Other There is an increasing interest in complementing standard RNA seq experiments with small RNA expression data to obtain a comprehensive view of the transcriptome. Two aspects need to be considered when small RNA seq sRNA seq is used. First sRNA seq protocols are size selective and typically optimized for sequencing miRNAs. Second given the dynamic composition of the small RNA fraction a reliable normalization strategy is needed to identify differentially expressed sRNAs. To address both issues we here present two sets of synthetic RNA spike in controls for monitoring size selectivity and for performing normalization. Size spike ins comprising 18 oligoribonucleotides 10–300 nucleotides were designed and tested using controlled modifications to the size selectivity of sRNA seq. As an applied example the loss of RNA molecules <30 nucleotides during small RNA isolation from zebrafish oocytes is demonstrated. Normalization spike ins comprising 19 oligoribonucleotides were designed to use as an external reference for DESeq normalization. Spike in based normalization improved the quantification of predetermined fold changes. Lastly sRNA seq on female and male zebrafish demonstrated that the higher miRNA content in males was correctly preserved post spike in based normalization.       Male 001 SAMEA2796299 UNIVERSITY OF AMSTERDAM Alias:Male 001|ENA checklist:ERC000011|INSDC center alias:UNIVERSITY OF AMSTERDAM|INSDC center name:UNIVERSITY OF AMSTERDAM|INSDC first public:2014 11 29T17:02:28Z|INSDC last update:2014 10 01T14:42:44Z|INSDC status:public|SRA accession:ERS557914|Sample Name:ERS557914|Title:Male ZF|dev stage:Adult|sex:male|strain:ABxTL                 Ion Torrent Proton sequencing ena EXPERIMENT UNIVERSITY OF AMSTERDAM 01 10 2014 15:08:22:087 14 RID0017 EtOH conc. experiment     miRNA-Seq TRANSCRIPTOMIC cDNA SINGLE ION_TORRENT Ion Torrent Proton   ERP007147 Ion Torrent Proton sequencing ENA FIRST PUBLIC:2014 11 29|ENA LAST UPDATE:2018 11 16 RID0017_014.fastq.gz fastq 83983757.0 3823842.0 ena RUN UNIVERSITY OF AMSTERDAM 01 10 2014 15:08:22:087 14 0:21.96 A:22501534;C:16455147;G:21619305;T:23407771;N:0 21       22501534 16455147 21619305 23407771 0 ERX604065 ERS557914 ERA363851 UNIVERSITY OF AMSTERDAM UNIVERSITY OF AMSTERDAM 1 0.70211   0.13351   0.92038   0.45898   22   B   usable mapping rate ion_torrent ion_torrent unknown small_rna unknown bulk unknown unknown   Netherlands 2014-10-01 Adult Adult Undetermined Undetermined
15526 15526 ERR647631 ERX604067 ERS557914 ERP007147 PRJEB7420 Two Sets of Synthetic Spike in Controls for Size Selection and Data Normalisation in small RNA seq ena-STUDY-UNIVERSITY OF AMSTERDAM-01-10-2014-14:02:22:856-622 Other There is an increasing interest in complementing standard RNA seq experiments with small RNA expression data to obtain a comprehensive view of the transcriptome. Two aspects need to be considered when small RNA seq sRNA seq is used. First sRNA seq protocols are size selective and typically optimized for sequencing miRNAs. Second given the dynamic composition of the small RNA fraction a reliable normalization strategy is needed to identify differentially expressed sRNAs. To address both issues we here present two sets of synthetic RNA spike in controls for monitoring size selectivity and for performing normalization. Size spike ins comprising 18 oligoribonucleotides 10–300 nucleotides were designed and tested using controlled modifications to the size selectivity of sRNA seq. As an applied example the loss of RNA molecules <30 nucleotides during small RNA isolation from zebrafish oocytes is demonstrated. Normalization spike ins comprising 19 oligoribonucleotides were designed to use as an external reference for DESeq normalization. Spike in based normalization improved the quantification of predetermined fold changes. Lastly sRNA seq on female and male zebrafish demonstrated that the higher miRNA content in males was correctly preserved post spike in based normalization.       Male 001 SAMEA2796299 UNIVERSITY OF AMSTERDAM Alias:Male 001|ENA checklist:ERC000011|INSDC center alias:UNIVERSITY OF AMSTERDAM|INSDC center name:UNIVERSITY OF AMSTERDAM|INSDC first public:2014 11 29T17:02:28Z|INSDC last update:2014 10 01T14:42:44Z|INSDC status:public|SRA accession:ERS557914|Sample Name:ERS557914|Title:Male ZF|dev stage:Adult|sex:male|strain:ABxTL                 Ion Torrent Proton sequencing ena EXPERIMENT UNIVERSITY OF AMSTERDAM 01 10 2014 15:08:22:087 16 RID0017 EtOH conc. experiment     miRNA-Seq TRANSCRIPTOMIC cDNA SINGLE ION_TORRENT Ion Torrent Proton   ERP007147 Ion Torrent Proton sequencing ENA FIRST PUBLIC:2014 11 29|ENA LAST UPDATE:2018 11 16 RID0017_016.fastq.gz fastq 117220472.0 4272650.0 ena RUN UNIVERSITY OF AMSTERDAM 01 10 2014 15:08:22:087 16 0:27.44 A:29509123;C:25768315;G:30220006;T:31723028;N:0 27       29509123 25768315 30220006 31723028 0 ERX604067 ERS557914 ERA363851 UNIVERSITY OF AMSTERDAM UNIVERSITY OF AMSTERDAM 1 0.68585   0.14679   0.90778   0.49492   20   B   usable mapping rate ion_torrent ion_torrent unknown small_rna unknown bulk unknown unknown   Netherlands 2014-10-01 Adult Adult Undetermined Undetermined
15527 15527 ERR647618 ERX604054 ERS557914 ERP007147 PRJEB7420 Two Sets of Synthetic Spike in Controls for Size Selection and Data Normalisation in small RNA seq ena-STUDY-UNIVERSITY OF AMSTERDAM-01-10-2014-14:02:22:856-622 Other There is an increasing interest in complementing standard RNA seq experiments with small RNA expression data to obtain a comprehensive view of the transcriptome. Two aspects need to be considered when small RNA seq sRNA seq is used. First sRNA seq protocols are size selective and typically optimized for sequencing miRNAs. Second given the dynamic composition of the small RNA fraction a reliable normalization strategy is needed to identify differentially expressed sRNAs. To address both issues we here present two sets of synthetic RNA spike in controls for monitoring size selectivity and for performing normalization. Size spike ins comprising 18 oligoribonucleotides 10–300 nucleotides were designed and tested using controlled modifications to the size selectivity of sRNA seq. As an applied example the loss of RNA molecules <30 nucleotides during small RNA isolation from zebrafish oocytes is demonstrated. Normalization spike ins comprising 19 oligoribonucleotides were designed to use as an external reference for DESeq normalization. Spike in based normalization improved the quantification of predetermined fold changes. Lastly sRNA seq on female and male zebrafish demonstrated that the higher miRNA content in males was correctly preserved post spike in based normalization.       Male 001 SAMEA2796299 UNIVERSITY OF AMSTERDAM Alias:Male 001|ENA checklist:ERC000011|INSDC center alias:UNIVERSITY OF AMSTERDAM|INSDC center name:UNIVERSITY OF AMSTERDAM|INSDC first public:2014 11 29T17:02:28Z|INSDC last update:2014 10 01T14:42:44Z|INSDC status:public|SRA accession:ERS557914|Sample Name:ERS557914|Title:Male ZF|dev stage:Adult|sex:male|strain:ABxTL                 Ion Torrent Proton sequencing ena EXPERIMENT UNIVERSITY OF AMSTERDAM 01 10 2014 15:08:22:087 3 RID0017 EtOH conc. experiment     miRNA-Seq TRANSCRIPTOMIC cDNA SINGLE ION_TORRENT Ion Torrent Proton   ERP007147 Ion Torrent Proton sequencing ENA FIRST PUBLIC:2014 11 29|ENA LAST UPDATE:2018 11 16 RID0017_003.fastq.gz fastq 165279016.0 5211580.0 ena RUN UNIVERSITY OF AMSTERDAM 01 10 2014 15:08:22:087 3 0:31.71 A:41451579;C:36699133;G:41477197;T:45651107;N:0 31       41451579 36699133 41477197 45651107 0 ERX604054 ERS557914 ERA363851 UNIVERSITY OF AMSTERDAM UNIVERSITY OF AMSTERDAM 1 0.71827   0.17456   0.90554   0.50274   18   B   usable mapping rate ion_torrent ion_torrent unknown small_rna unknown bulk unknown unknown   Netherlands 2014-10-01 Adult Adult Undetermined Undetermined
15528 15528 ERR647621 ERX604057 ERS557914 ERP007147 PRJEB7420 Two Sets of Synthetic Spike in Controls for Size Selection and Data Normalisation in small RNA seq ena-STUDY-UNIVERSITY OF AMSTERDAM-01-10-2014-14:02:22:856-622 Other There is an increasing interest in complementing standard RNA seq experiments with small RNA expression data to obtain a comprehensive view of the transcriptome. Two aspects need to be considered when small RNA seq sRNA seq is used. First sRNA seq protocols are size selective and typically optimized for sequencing miRNAs. Second given the dynamic composition of the small RNA fraction a reliable normalization strategy is needed to identify differentially expressed sRNAs. To address both issues we here present two sets of synthetic RNA spike in controls for monitoring size selectivity and for performing normalization. Size spike ins comprising 18 oligoribonucleotides 10–300 nucleotides were designed and tested using controlled modifications to the size selectivity of sRNA seq. As an applied example the loss of RNA molecules <30 nucleotides during small RNA isolation from zebrafish oocytes is demonstrated. Normalization spike ins comprising 19 oligoribonucleotides were designed to use as an external reference for DESeq normalization. Spike in based normalization improved the quantification of predetermined fold changes. Lastly sRNA seq on female and male zebrafish demonstrated that the higher miRNA content in males was correctly preserved post spike in based normalization.       Male 001 SAMEA2796299 UNIVERSITY OF AMSTERDAM Alias:Male 001|ENA checklist:ERC000011|INSDC center alias:UNIVERSITY OF AMSTERDAM|INSDC center name:UNIVERSITY OF AMSTERDAM|INSDC first public:2014 11 29T17:02:28Z|INSDC last update:2014 10 01T14:42:44Z|INSDC status:public|SRA accession:ERS557914|Sample Name:ERS557914|Title:Male ZF|dev stage:Adult|sex:male|strain:ABxTL                 Ion Torrent Proton sequencing ena EXPERIMENT UNIVERSITY OF AMSTERDAM 01 10 2014 15:08:22:087 6 RID0017 EtOH conc. experiment     miRNA-Seq TRANSCRIPTOMIC cDNA SINGLE ION_TORRENT Ion Torrent Proton   ERP007147 Ion Torrent Proton sequencing ENA FIRST PUBLIC:2014 11 29|ENA LAST UPDATE:2018 11 16 RID0017_006.fastq.gz fastq 181164500.0 7686285.0 ena RUN UNIVERSITY OF AMSTERDAM 01 10 2014 15:08:22:087 6 0:23.57 A:48622049;C:36019225;G:45318073;T:51205153;N:0 23       48622049 36019225 45318073 51205153 0 ERX604057 ERS557914 ERA363851 UNIVERSITY OF AMSTERDAM UNIVERSITY OF AMSTERDAM 1 0.73267   0.15948   0.9026   0.483   14   B   usable mapping rate ion_torrent ion_torrent unknown small_rna unknown bulk unknown unknown   Netherlands 2014-10-01 Adult Adult Undetermined Undetermined
15529 15529 ERR647619 ERX604055 ERS557914 ERP007147 PRJEB7420 Two Sets of Synthetic Spike in Controls for Size Selection and Data Normalisation in small RNA seq ena-STUDY-UNIVERSITY OF AMSTERDAM-01-10-2014-14:02:22:856-622 Other There is an increasing interest in complementing standard RNA seq experiments with small RNA expression data to obtain a comprehensive view of the transcriptome. Two aspects need to be considered when small RNA seq sRNA seq is used. First sRNA seq protocols are size selective and typically optimized for sequencing miRNAs. Second given the dynamic composition of the small RNA fraction a reliable normalization strategy is needed to identify differentially expressed sRNAs. To address both issues we here present two sets of synthetic RNA spike in controls for monitoring size selectivity and for performing normalization. Size spike ins comprising 18 oligoribonucleotides 10–300 nucleotides were designed and tested using controlled modifications to the size selectivity of sRNA seq. As an applied example the loss of RNA molecules <30 nucleotides during small RNA isolation from zebrafish oocytes is demonstrated. Normalization spike ins comprising 19 oligoribonucleotides were designed to use as an external reference for DESeq normalization. Spike in based normalization improved the quantification of predetermined fold changes. Lastly sRNA seq on female and male zebrafish demonstrated that the higher miRNA content in males was correctly preserved post spike in based normalization.       Male 001 SAMEA2796299 UNIVERSITY OF AMSTERDAM Alias:Male 001|ENA checklist:ERC000011|INSDC center alias:UNIVERSITY OF AMSTERDAM|INSDC center name:UNIVERSITY OF AMSTERDAM|INSDC first public:2014 11 29T17:02:28Z|INSDC last update:2014 10 01T14:42:44Z|INSDC status:public|SRA accession:ERS557914|Sample Name:ERS557914|Title:Male ZF|dev stage:Adult|sex:male|strain:ABxTL                 Ion Torrent Proton sequencing ena EXPERIMENT UNIVERSITY OF AMSTERDAM 01 10 2014 15:08:22:087 4 RID0017 EtOH conc. experiment     miRNA-Seq TRANSCRIPTOMIC cDNA SINGLE ION_TORRENT Ion Torrent Proton   ERP007147 Ion Torrent Proton sequencing ENA FIRST PUBLIC:2014 11 29|ENA LAST UPDATE:2018 11 16 RID0017_004.fastq.gz fastq 131702671.0 4688094.0 ena RUN UNIVERSITY OF AMSTERDAM 01 10 2014 15:08:22:087 4 0:28.09 A:32537215;C:28853793;G:34317576;T:35994087;N:0 28       32537215 28853793 34317576 35994087 0 ERX604055 ERS557914 ERA363851 UNIVERSITY OF AMSTERDAM UNIVERSITY OF AMSTERDAM 1 0.68635   0.14911   0.90532   0.50027   31   B   usable mapping rate ion_torrent ion_torrent unknown small_rna unknown bulk unknown unknown   Netherlands 2014-10-01 Adult Adult Undetermined Undetermined
15530 15530 ERR647628 ERX604064 ERS557914 ERP007147 PRJEB7420 Two Sets of Synthetic Spike in Controls for Size Selection and Data Normalisation in small RNA seq ena-STUDY-UNIVERSITY OF AMSTERDAM-01-10-2014-14:02:22:856-622 Other There is an increasing interest in complementing standard RNA seq experiments with small RNA expression data to obtain a comprehensive view of the transcriptome. Two aspects need to be considered when small RNA seq sRNA seq is used. First sRNA seq protocols are size selective and typically optimized for sequencing miRNAs. Second given the dynamic composition of the small RNA fraction a reliable normalization strategy is needed to identify differentially expressed sRNAs. To address both issues we here present two sets of synthetic RNA spike in controls for monitoring size selectivity and for performing normalization. Size spike ins comprising 18 oligoribonucleotides 10–300 nucleotides were designed and tested using controlled modifications to the size selectivity of sRNA seq. As an applied example the loss of RNA molecules <30 nucleotides during small RNA isolation from zebrafish oocytes is demonstrated. Normalization spike ins comprising 19 oligoribonucleotides were designed to use as an external reference for DESeq normalization. Spike in based normalization improved the quantification of predetermined fold changes. Lastly sRNA seq on female and male zebrafish demonstrated that the higher miRNA content in males was correctly preserved post spike in based normalization.       Male 001 SAMEA2796299 UNIVERSITY OF AMSTERDAM Alias:Male 001|ENA checklist:ERC000011|INSDC center alias:UNIVERSITY OF AMSTERDAM|INSDC center name:UNIVERSITY OF AMSTERDAM|INSDC first public:2014 11 29T17:02:28Z|INSDC last update:2014 10 01T14:42:44Z|INSDC status:public|SRA accession:ERS557914|Sample Name:ERS557914|Title:Male ZF|dev stage:Adult|sex:male|strain:ABxTL                 Ion Torrent Proton sequencing ena EXPERIMENT UNIVERSITY OF AMSTERDAM 01 10 2014 15:08:22:087 13 RID0017 EtOH conc. experiment     miRNA-Seq TRANSCRIPTOMIC cDNA SINGLE ION_TORRENT Ion Torrent Proton   ERP007147 Ion Torrent Proton sequencing ENA FIRST PUBLIC:2014 11 29|ENA LAST UPDATE:2018 11 16 RID0017_013.fastq.gz fastq 123881257.0 5143681.0 ena RUN UNIVERSITY OF AMSTERDAM 01 10 2014 15:08:22:087 13 0:24.08 A:32852368;C:25503683;G:31339781;T:34185425;N:0 24       32852368 25503683 31339781 34185425 0 ERX604064 ERS557914 ERA363851 UNIVERSITY OF AMSTERDAM UNIVERSITY OF AMSTERDAM 1 0.72384   0.15147   0.90648   0.46   24   B   usable mapping rate ion_torrent ion_torrent unknown small_rna unknown bulk unknown unknown   Netherlands 2014-10-01 Adult Adult Undetermined Undetermined
15531 15531 ERR647627 ERX604063 ERS557914 ERP007147 PRJEB7420 Two Sets of Synthetic Spike in Controls for Size Selection and Data Normalisation in small RNA seq ena-STUDY-UNIVERSITY OF AMSTERDAM-01-10-2014-14:02:22:856-622 Other There is an increasing interest in complementing standard RNA seq experiments with small RNA expression data to obtain a comprehensive view of the transcriptome. Two aspects need to be considered when small RNA seq sRNA seq is used. First sRNA seq protocols are size selective and typically optimized for sequencing miRNAs. Second given the dynamic composition of the small RNA fraction a reliable normalization strategy is needed to identify differentially expressed sRNAs. To address both issues we here present two sets of synthetic RNA spike in controls for monitoring size selectivity and for performing normalization. Size spike ins comprising 18 oligoribonucleotides 10–300 nucleotides were designed and tested using controlled modifications to the size selectivity of sRNA seq. As an applied example the loss of RNA molecules <30 nucleotides during small RNA isolation from zebrafish oocytes is demonstrated. Normalization spike ins comprising 19 oligoribonucleotides were designed to use as an external reference for DESeq normalization. Spike in based normalization improved the quantification of predetermined fold changes. Lastly sRNA seq on female and male zebrafish demonstrated that the higher miRNA content in males was correctly preserved post spike in based normalization.       Male 001 SAMEA2796299 UNIVERSITY OF AMSTERDAM Alias:Male 001|ENA checklist:ERC000011|INSDC center alias:UNIVERSITY OF AMSTERDAM|INSDC center name:UNIVERSITY OF AMSTERDAM|INSDC first public:2014 11 29T17:02:28Z|INSDC last update:2014 10 01T14:42:44Z|INSDC status:public|SRA accession:ERS557914|Sample Name:ERS557914|Title:Male ZF|dev stage:Adult|sex:male|strain:ABxTL                 Ion Torrent Proton sequencing ena EXPERIMENT UNIVERSITY OF AMSTERDAM 01 10 2014 15:08:22:087 12 RID0017 EtOH conc. experiment     miRNA-Seq TRANSCRIPTOMIC cDNA SINGLE ION_TORRENT Ion Torrent Proton   ERP007147 Ion Torrent Proton sequencing ENA FIRST PUBLIC:2014 11 29|ENA LAST UPDATE:2018 11 16 RID0017_012.fastq.gz fastq 135155439.0 6123293.0 ena RUN UNIVERSITY OF AMSTERDAM 01 10 2014 15:08:22:087 12 0:22.07 A:35870227;C:27070386;G:34401412;T:37813414;N:0 22       35870227 27070386 34401412 37813414 0 ERX604063 ERS557914 ERA363851 UNIVERSITY OF AMSTERDAM UNIVERSITY OF AMSTERDAM 1 0.69126   0.12647   0.91695   0.46572   25   B   usable mapping rate ion_torrent ion_torrent unknown small_rna unknown bulk unknown unknown   Netherlands 2014-10-01 Adult Adult Undetermined Undetermined
15532 15532 ERR647626 ERX604062 ERS557914 ERP007147 PRJEB7420 Two Sets of Synthetic Spike in Controls for Size Selection and Data Normalisation in small RNA seq ena-STUDY-UNIVERSITY OF AMSTERDAM-01-10-2014-14:02:22:856-622 Other There is an increasing interest in complementing standard RNA seq experiments with small RNA expression data to obtain a comprehensive view of the transcriptome. Two aspects need to be considered when small RNA seq sRNA seq is used. First sRNA seq protocols are size selective and typically optimized for sequencing miRNAs. Second given the dynamic composition of the small RNA fraction a reliable normalization strategy is needed to identify differentially expressed sRNAs. To address both issues we here present two sets of synthetic RNA spike in controls for monitoring size selectivity and for performing normalization. Size spike ins comprising 18 oligoribonucleotides 10–300 nucleotides were designed and tested using controlled modifications to the size selectivity of sRNA seq. As an applied example the loss of RNA molecules <30 nucleotides during small RNA isolation from zebrafish oocytes is demonstrated. Normalization spike ins comprising 19 oligoribonucleotides were designed to use as an external reference for DESeq normalization. Spike in based normalization improved the quantification of predetermined fold changes. Lastly sRNA seq on female and male zebrafish demonstrated that the higher miRNA content in males was correctly preserved post spike in based normalization.       Male 001 SAMEA2796299 UNIVERSITY OF AMSTERDAM Alias:Male 001|ENA checklist:ERC000011|INSDC center alias:UNIVERSITY OF AMSTERDAM|INSDC center name:UNIVERSITY OF AMSTERDAM|INSDC first public:2014 11 29T17:02:28Z|INSDC last update:2014 10 01T14:42:44Z|INSDC status:public|SRA accession:ERS557914|Sample Name:ERS557914|Title:Male ZF|dev stage:Adult|sex:male|strain:ABxTL                 Ion Torrent Proton sequencing ena EXPERIMENT UNIVERSITY OF AMSTERDAM 01 10 2014 15:08:22:087 11 RID0017 EtOH conc. experiment     miRNA-Seq TRANSCRIPTOMIC cDNA SINGLE ION_TORRENT Ion Torrent Proton   ERP007147 Ion Torrent Proton sequencing ENA FIRST PUBLIC:2014 11 29|ENA LAST UPDATE:2018 11 16 RID0017_011.fastq.gz fastq 138836612.0 5648801.0 ena RUN UNIVERSITY OF AMSTERDAM 01 10 2014 15:08:22:087 11 0:24.58 A:36426455;C:28359755;G:34985736;T:39064666;N:0 24       36426455 28359755 34985736 39064666 0 ERX604062 ERS557914 ERA363851 UNIVERSITY OF AMSTERDAM UNIVERSITY OF AMSTERDAM 1 0.73565   0.15718   0.90382   0.45772   67   B   usable mapping rate ion_torrent ion_torrent unknown small_rna unknown bulk unknown unknown   Netherlands 2014-10-01 Adult Adult Undetermined Undetermined
15533 15533 ERR647625 ERX604061 ERS557914 ERP007147 PRJEB7420 Two Sets of Synthetic Spike in Controls for Size Selection and Data Normalisation in small RNA seq ena-STUDY-UNIVERSITY OF AMSTERDAM-01-10-2014-14:02:22:856-622 Other There is an increasing interest in complementing standard RNA seq experiments with small RNA expression data to obtain a comprehensive view of the transcriptome. Two aspects need to be considered when small RNA seq sRNA seq is used. First sRNA seq protocols are size selective and typically optimized for sequencing miRNAs. Second given the dynamic composition of the small RNA fraction a reliable normalization strategy is needed to identify differentially expressed sRNAs. To address both issues we here present two sets of synthetic RNA spike in controls for monitoring size selectivity and for performing normalization. Size spike ins comprising 18 oligoribonucleotides 10–300 nucleotides were designed and tested using controlled modifications to the size selectivity of sRNA seq. As an applied example the loss of RNA molecules <30 nucleotides during small RNA isolation from zebrafish oocytes is demonstrated. Normalization spike ins comprising 19 oligoribonucleotides were designed to use as an external reference for DESeq normalization. Spike in based normalization improved the quantification of predetermined fold changes. Lastly sRNA seq on female and male zebrafish demonstrated that the higher miRNA content in males was correctly preserved post spike in based normalization.       Male 001 SAMEA2796299 UNIVERSITY OF AMSTERDAM Alias:Male 001|ENA checklist:ERC000011|INSDC center alias:UNIVERSITY OF AMSTERDAM|INSDC center name:UNIVERSITY OF AMSTERDAM|INSDC first public:2014 11 29T17:02:28Z|INSDC last update:2014 10 01T14:42:44Z|INSDC status:public|SRA accession:ERS557914|Sample Name:ERS557914|Title:Male ZF|dev stage:Adult|sex:male|strain:ABxTL                 Ion Torrent Proton sequencing ena EXPERIMENT UNIVERSITY OF AMSTERDAM 01 10 2014 15:08:22:087 10 RID0017 EtOH conc. experiment     miRNA-Seq TRANSCRIPTOMIC cDNA SINGLE ION_TORRENT Ion Torrent Proton   ERP007147 Ion Torrent Proton sequencing ENA FIRST PUBLIC:2014 11 29|ENA LAST UPDATE:2018 11 16 RID0017_010.fastq.gz fastq 182404716.0 6612019.0 ena RUN UNIVERSITY OF AMSTERDAM 01 10 2014 15:08:22:087 10 0:27.59 A:47718609;C:38775928;G:45637186;T:50272993;N:0 27       47718609 38775928 45637186 50272993 0 ERX604061 ERS557914 ERA363851 UNIVERSITY OF AMSTERDAM UNIVERSITY OF AMSTERDAM 1 0.75246   0.19746   0.88544   0.48212   36   B   usable mapping rate ion_torrent ion_torrent unknown small_rna unknown bulk unknown unknown   Netherlands 2014-10-01 Adult Adult Undetermined Undetermined
15534 15534 ERR647616 ERX604052 ERS557914 ERP007147 PRJEB7420 Two Sets of Synthetic Spike in Controls for Size Selection and Data Normalisation in small RNA seq ena-STUDY-UNIVERSITY OF AMSTERDAM-01-10-2014-14:02:22:856-622 Other There is an increasing interest in complementing standard RNA seq experiments with small RNA expression data to obtain a comprehensive view of the transcriptome. Two aspects need to be considered when small RNA seq sRNA seq is used. First sRNA seq protocols are size selective and typically optimized for sequencing miRNAs. Second given the dynamic composition of the small RNA fraction a reliable normalization strategy is needed to identify differentially expressed sRNAs. To address both issues we here present two sets of synthetic RNA spike in controls for monitoring size selectivity and for performing normalization. Size spike ins comprising 18 oligoribonucleotides 10–300 nucleotides were designed and tested using controlled modifications to the size selectivity of sRNA seq. As an applied example the loss of RNA molecules <30 nucleotides during small RNA isolation from zebrafish oocytes is demonstrated. Normalization spike ins comprising 19 oligoribonucleotides were designed to use as an external reference for DESeq normalization. Spike in based normalization improved the quantification of predetermined fold changes. Lastly sRNA seq on female and male zebrafish demonstrated that the higher miRNA content in males was correctly preserved post spike in based normalization.       Male 001 SAMEA2796299 UNIVERSITY OF AMSTERDAM Alias:Male 001|ENA checklist:ERC000011|INSDC center alias:UNIVERSITY OF AMSTERDAM|INSDC center name:UNIVERSITY OF AMSTERDAM|INSDC first public:2014 11 29T17:02:28Z|INSDC last update:2014 10 01T14:42:44Z|INSDC status:public|SRA accession:ERS557914|Sample Name:ERS557914|Title:Male ZF|dev stage:Adult|sex:male|strain:ABxTL                 Ion Torrent Proton sequencing ena EXPERIMENT UNIVERSITY OF AMSTERDAM 01 10 2014 15:08:22:086 1 RID0017 EtOH conc. experiment     miRNA-Seq TRANSCRIPTOMIC cDNA SINGLE ION_TORRENT Ion Torrent Proton   ERP007147 Ion Torrent Proton sequencing ENA FIRST PUBLIC:2014 11 29|ENA LAST UPDATE:2018 11 16 RID0017_001.fastq.gz fastq 137653436.0 5155975.0 ena RUN UNIVERSITY OF AMSTERDAM 01 10 2014 15:08:22:087 1 0:26.70 A:34561717;C:29961559;G:35320222;T:37809938;N:0 26       34561717 29961559 35320222 37809938 0 ERX604052 ERS557914 ERA363851 UNIVERSITY OF AMSTERDAM UNIVERSITY OF AMSTERDAM 1 0.71887   0.15223   0.90824   0.49462   22   B   usable mapping rate ion_torrent ion_torrent unknown small_rna unknown bulk unknown unknown   Netherlands 2014-10-01 Adult Adult Undetermined Undetermined
15535 15535 ERR647630 ERX604066 ERS557914 ERP007147 PRJEB7420 Two Sets of Synthetic Spike in Controls for Size Selection and Data Normalisation in small RNA seq ena-STUDY-UNIVERSITY OF AMSTERDAM-01-10-2014-14:02:22:856-622 Other There is an increasing interest in complementing standard RNA seq experiments with small RNA expression data to obtain a comprehensive view of the transcriptome. Two aspects need to be considered when small RNA seq sRNA seq is used. First sRNA seq protocols are size selective and typically optimized for sequencing miRNAs. Second given the dynamic composition of the small RNA fraction a reliable normalization strategy is needed to identify differentially expressed sRNAs. To address both issues we here present two sets of synthetic RNA spike in controls for monitoring size selectivity and for performing normalization. Size spike ins comprising 18 oligoribonucleotides 10–300 nucleotides were designed and tested using controlled modifications to the size selectivity of sRNA seq. As an applied example the loss of RNA molecules <30 nucleotides during small RNA isolation from zebrafish oocytes is demonstrated. Normalization spike ins comprising 19 oligoribonucleotides were designed to use as an external reference for DESeq normalization. Spike in based normalization improved the quantification of predetermined fold changes. Lastly sRNA seq on female and male zebrafish demonstrated that the higher miRNA content in males was correctly preserved post spike in based normalization.       Male 001 SAMEA2796299 UNIVERSITY OF AMSTERDAM Alias:Male 001|ENA checklist:ERC000011|INSDC center alias:UNIVERSITY OF AMSTERDAM|INSDC center name:UNIVERSITY OF AMSTERDAM|INSDC first public:2014 11 29T17:02:28Z|INSDC last update:2014 10 01T14:42:44Z|INSDC status:public|SRA accession:ERS557914|Sample Name:ERS557914|Title:Male ZF|dev stage:Adult|sex:male|strain:ABxTL                 Ion Torrent Proton sequencing ena EXPERIMENT UNIVERSITY OF AMSTERDAM 01 10 2014 15:08:22:087 15 RID0017 EtOH conc. experiment     miRNA-Seq TRANSCRIPTOMIC cDNA SINGLE ION_TORRENT Ion Torrent Proton   ERP007147 Ion Torrent Proton sequencing ENA FIRST PUBLIC:2014 11 29|ENA LAST UPDATE:2018 11 16 RID0017_015.fastq.gz fastq 101714594.0 4045987.0 ena RUN UNIVERSITY OF AMSTERDAM 01 10 2014 15:08:22:087 15 0:25.14 A:26518632;C:20741074;G:25853629;T:28601259;N:0 25       26518632 20741074 25853629 28601259 0 ERX604066 ERS557914 ERA363851 UNIVERSITY OF AMSTERDAM UNIVERSITY OF AMSTERDAM 1 0.74566   0.16455   0.89534   0.48977   22   B   usable mapping rate ion_torrent ion_torrent unknown small_rna unknown bulk unknown unknown   Netherlands 2014-10-01 Adult Adult Undetermined Undetermined
15536 15536 ERR647610 ERX604046 ERS557914 ERP007147 PRJEB7420 Two Sets of Synthetic Spike in Controls for Size Selection and Data Normalisation in small RNA seq ena-STUDY-UNIVERSITY OF AMSTERDAM-01-10-2014-14:02:22:856-622 Other There is an increasing interest in complementing standard RNA seq experiments with small RNA expression data to obtain a comprehensive view of the transcriptome. Two aspects need to be considered when small RNA seq sRNA seq is used. First sRNA seq protocols are size selective and typically optimized for sequencing miRNAs. Second given the dynamic composition of the small RNA fraction a reliable normalization strategy is needed to identify differentially expressed sRNAs. To address both issues we here present two sets of synthetic RNA spike in controls for monitoring size selectivity and for performing normalization. Size spike ins comprising 18 oligoribonucleotides 10–300 nucleotides were designed and tested using controlled modifications to the size selectivity of sRNA seq. As an applied example the loss of RNA molecules <30 nucleotides during small RNA isolation from zebrafish oocytes is demonstrated. Normalization spike ins comprising 19 oligoribonucleotides were designed to use as an external reference for DESeq normalization. Spike in based normalization improved the quantification of predetermined fold changes. Lastly sRNA seq on female and male zebrafish demonstrated that the higher miRNA content in males was correctly preserved post spike in based normalization.       Male 001 SAMEA2796299 UNIVERSITY OF AMSTERDAM Alias:Male 001|ENA checklist:ERC000011|INSDC center alias:UNIVERSITY OF AMSTERDAM|INSDC center name:UNIVERSITY OF AMSTERDAM|INSDC first public:2014 11 29T17:02:28Z|INSDC last update:2014 10 01T14:42:44Z|INSDC status:public|SRA accession:ERS557914|Sample Name:ERS557914|Title:Male ZF|dev stage:Adult|sex:male|strain:ABxTL                 Ion Torrent PGM sequencing ena EXPERIMENT UNIVERSITY OF AMSTERDAM 01 10 2014 14:59:02:785 1 RID0023 1     miRNA-Seq TRANSCRIPTOMIC cDNA SINGLE ION_TORRENT Ion Torrent PGM   ERP007147 Ion Torrent PGM sequencing ENA FIRST PUBLIC:2014 11 29|ENA LAST UPDATE:2018 11 16 RID0023_009.fastq.gz fastq 114880508.0 4321457.0 ena RUN UNIVERSITY OF AMSTERDAM 01 10 2014 14:59:02:785 1 0:26.58 A:31499097;C:23030789;G:27803371;T:32547251;N:0 26       31499097 23030789 27803371 32547251 0 ERX604046 ERS557914 ERA363850 UNIVERSITY OF AMSTERDAM UNIVERSITY OF AMSTERDAM 1 0.7932   0.2086   0.89045   0.49364   22   B   usable mapping rate ion_torrent ion_torrent unknown small_rna unknown bulk unknown unknown   Netherlands 2014-10-01 Adult Adult Undetermined Undetermined
15537 15537 ERR647611 ERX604047 ERS557914 ERP007147 PRJEB7420 Two Sets of Synthetic Spike in Controls for Size Selection and Data Normalisation in small RNA seq ena-STUDY-UNIVERSITY OF AMSTERDAM-01-10-2014-14:02:22:856-622 Other There is an increasing interest in complementing standard RNA seq experiments with small RNA expression data to obtain a comprehensive view of the transcriptome. Two aspects need to be considered when small RNA seq sRNA seq is used. First sRNA seq protocols are size selective and typically optimized for sequencing miRNAs. Second given the dynamic composition of the small RNA fraction a reliable normalization strategy is needed to identify differentially expressed sRNAs. To address both issues we here present two sets of synthetic RNA spike in controls for monitoring size selectivity and for performing normalization. Size spike ins comprising 18 oligoribonucleotides 10–300 nucleotides were designed and tested using controlled modifications to the size selectivity of sRNA seq. As an applied example the loss of RNA molecules <30 nucleotides during small RNA isolation from zebrafish oocytes is demonstrated. Normalization spike ins comprising 19 oligoribonucleotides were designed to use as an external reference for DESeq normalization. Spike in based normalization improved the quantification of predetermined fold changes. Lastly sRNA seq on female and male zebrafish demonstrated that the higher miRNA content in males was correctly preserved post spike in based normalization.       Male 001 SAMEA2796299 UNIVERSITY OF AMSTERDAM Alias:Male 001|ENA checklist:ERC000011|INSDC center alias:UNIVERSITY OF AMSTERDAM|INSDC center name:UNIVERSITY OF AMSTERDAM|INSDC first public:2014 11 29T17:02:28Z|INSDC last update:2014 10 01T14:42:44Z|INSDC status:public|SRA accession:ERS557914|Sample Name:ERS557914|Title:Male ZF|dev stage:Adult|sex:male|strain:ABxTL                 Ion Torrent Proton sequencing ena EXPERIMENT UNIVERSITY OF AMSTERDAM 01 10 2014 14:59:02:785 2 RID0023 1     miRNA-Seq TRANSCRIPTOMIC cDNA SINGLE ION_TORRENT Ion Torrent Proton   ERP007147 Ion Torrent Proton sequencing ENA FIRST PUBLIC:2014 11 29|ENA LAST UPDATE:2018 11 16 RID0023_010.fastq.gz fastq 124380158.0 4885304.0 ena RUN UNIVERSITY OF AMSTERDAM 01 10 2014 14:59:02:785 2 0:25.46 A:33809369;C:25116497;G:30529372;T:34924920;N:0 25       33809369 25116497 30529372 34924920 0 ERX604047 ERS557914 ERA363850 UNIVERSITY OF AMSTERDAM UNIVERSITY OF AMSTERDAM 1 0.78302   0.1944   0.89398   0.50469   22   B   usable mapping rate ion_torrent ion_torrent unknown small_rna unknown bulk unknown unknown   Netherlands 2014-10-01 Adult Adult Undetermined Undetermined
15538 15538 ERR647612 ERX604048 ERS557914 ERP007147 PRJEB7420 Two Sets of Synthetic Spike in Controls for Size Selection and Data Normalisation in small RNA seq ena-STUDY-UNIVERSITY OF AMSTERDAM-01-10-2014-14:02:22:856-622 Other There is an increasing interest in complementing standard RNA seq experiments with small RNA expression data to obtain a comprehensive view of the transcriptome. Two aspects need to be considered when small RNA seq sRNA seq is used. First sRNA seq protocols are size selective and typically optimized for sequencing miRNAs. Second given the dynamic composition of the small RNA fraction a reliable normalization strategy is needed to identify differentially expressed sRNAs. To address both issues we here present two sets of synthetic RNA spike in controls for monitoring size selectivity and for performing normalization. Size spike ins comprising 18 oligoribonucleotides 10–300 nucleotides were designed and tested using controlled modifications to the size selectivity of sRNA seq. As an applied example the loss of RNA molecules <30 nucleotides during small RNA isolation from zebrafish oocytes is demonstrated. Normalization spike ins comprising 19 oligoribonucleotides were designed to use as an external reference for DESeq normalization. Spike in based normalization improved the quantification of predetermined fold changes. Lastly sRNA seq on female and male zebrafish demonstrated that the higher miRNA content in males was correctly preserved post spike in based normalization.       Male 001 SAMEA2796299 UNIVERSITY OF AMSTERDAM Alias:Male 001|ENA checklist:ERC000011|INSDC center alias:UNIVERSITY OF AMSTERDAM|INSDC center name:UNIVERSITY OF AMSTERDAM|INSDC first public:2014 11 29T17:02:28Z|INSDC last update:2014 10 01T14:42:44Z|INSDC status:public|SRA accession:ERS557914|Sample Name:ERS557914|Title:Male ZF|dev stage:Adult|sex:male|strain:ABxTL                 Ion Torrent Proton sequencing ena EXPERIMENT UNIVERSITY OF AMSTERDAM 01 10 2014 14:59:02:785 3 RID0023 1     miRNA-Seq TRANSCRIPTOMIC cDNA SINGLE ION_TORRENT Ion Torrent Proton   ERP007147 Ion Torrent Proton sequencing ENA FIRST PUBLIC:2014 11 29|ENA LAST UPDATE:2018 11 16 RID0023_011.fastq.gz fastq 122081664.0 4959591.0 ena RUN UNIVERSITY OF AMSTERDAM 01 10 2014 14:59:02:785 3 0:24.62 A:33452935;C:24151113;G:29942213;T:34535403;N:0 24       33452935 24151113 29942213 34535403 0 ERX604048 ERS557914 ERA363850 UNIVERSITY OF AMSTERDAM UNIVERSITY OF AMSTERDAM 1 0.77425   0.18982   0.8911   0.46046   22   B   usable mapping rate ion_torrent ion_torrent unknown small_rna unknown bulk unknown unknown   Netherlands 2014-10-01 Adult Adult Undetermined Undetermined
15539 15539 ERR647615 ERX604051 ERS557914 ERP007147 PRJEB7420 Two Sets of Synthetic Spike in Controls for Size Selection and Data Normalisation in small RNA seq ena-STUDY-UNIVERSITY OF AMSTERDAM-01-10-2014-14:02:22:856-622 Other There is an increasing interest in complementing standard RNA seq experiments with small RNA expression data to obtain a comprehensive view of the transcriptome. Two aspects need to be considered when small RNA seq sRNA seq is used. First sRNA seq protocols are size selective and typically optimized for sequencing miRNAs. Second given the dynamic composition of the small RNA fraction a reliable normalization strategy is needed to identify differentially expressed sRNAs. To address both issues we here present two sets of synthetic RNA spike in controls for monitoring size selectivity and for performing normalization. Size spike ins comprising 18 oligoribonucleotides 10–300 nucleotides were designed and tested using controlled modifications to the size selectivity of sRNA seq. As an applied example the loss of RNA molecules <30 nucleotides during small RNA isolation from zebrafish oocytes is demonstrated. Normalization spike ins comprising 19 oligoribonucleotides were designed to use as an external reference for DESeq normalization. Spike in based normalization improved the quantification of predetermined fold changes. Lastly sRNA seq on female and male zebrafish demonstrated that the higher miRNA content in males was correctly preserved post spike in based normalization.       Male 001 SAMEA2796299 UNIVERSITY OF AMSTERDAM Alias:Male 001|ENA checklist:ERC000011|INSDC center alias:UNIVERSITY OF AMSTERDAM|INSDC center name:UNIVERSITY OF AMSTERDAM|INSDC first public:2014 11 29T17:02:28Z|INSDC last update:2014 10 01T14:42:44Z|INSDC status:public|SRA accession:ERS557914|Sample Name:ERS557914|Title:Male ZF|dev stage:Adult|sex:male|strain:ABxTL                 Ion Torrent Proton sequencing ena EXPERIMENT UNIVERSITY OF AMSTERDAM 01 10 2014 14:59:02:785 6 RID0023 1     miRNA-Seq TRANSCRIPTOMIC cDNA SINGLE ION_TORRENT Ion Torrent Proton   ERP007147 Ion Torrent Proton sequencing ENA FIRST PUBLIC:2014 11 29|ENA LAST UPDATE:2018 11 16 RID0023_014.fastq.gz fastq 120491210.0 4564333.0 ena RUN UNIVERSITY OF AMSTERDAM 01 10 2014 14:59:02:785 6 0:26.40 A:33116448;C:24416657;G:29447901;T:33510204;N:0 26       33116448 24416657 29447901 33510204 0 ERX604051 ERS557914 ERA363850 UNIVERSITY OF AMSTERDAM UNIVERSITY OF AMSTERDAM 1 0.77843   0.20568   0.88605   0.51892   22   B   usable mapping rate ion_torrent ion_torrent unknown small_rna unknown bulk unknown unknown   Netherlands 2014-10-01 Adult Adult Undetermined Undetermined
15540 15540 ERR647614 ERX604050 ERS557914 ERP007147 PRJEB7420 Two Sets of Synthetic Spike in Controls for Size Selection and Data Normalisation in small RNA seq ena-STUDY-UNIVERSITY OF AMSTERDAM-01-10-2014-14:02:22:856-622 Other There is an increasing interest in complementing standard RNA seq experiments with small RNA expression data to obtain a comprehensive view of the transcriptome. Two aspects need to be considered when small RNA seq sRNA seq is used. First sRNA seq protocols are size selective and typically optimized for sequencing miRNAs. Second given the dynamic composition of the small RNA fraction a reliable normalization strategy is needed to identify differentially expressed sRNAs. To address both issues we here present two sets of synthetic RNA spike in controls for monitoring size selectivity and for performing normalization. Size spike ins comprising 18 oligoribonucleotides 10–300 nucleotides were designed and tested using controlled modifications to the size selectivity of sRNA seq. As an applied example the loss of RNA molecules <30 nucleotides during small RNA isolation from zebrafish oocytes is demonstrated. Normalization spike ins comprising 19 oligoribonucleotides were designed to use as an external reference for DESeq normalization. Spike in based normalization improved the quantification of predetermined fold changes. Lastly sRNA seq on female and male zebrafish demonstrated that the higher miRNA content in males was correctly preserved post spike in based normalization.       Male 001 SAMEA2796299 UNIVERSITY OF AMSTERDAM Alias:Male 001|ENA checklist:ERC000011|INSDC center alias:UNIVERSITY OF AMSTERDAM|INSDC center name:UNIVERSITY OF AMSTERDAM|INSDC first public:2014 11 29T17:02:28Z|INSDC last update:2014 10 01T14:42:44Z|INSDC status:public|SRA accession:ERS557914|Sample Name:ERS557914|Title:Male ZF|dev stage:Adult|sex:male|strain:ABxTL                 Ion Torrent Proton sequencing ena EXPERIMENT UNIVERSITY OF AMSTERDAM 01 10 2014 14:59:02:785 5 RID0023 1     miRNA-Seq TRANSCRIPTOMIC cDNA SINGLE ION_TORRENT Ion Torrent Proton   ERP007147 Ion Torrent Proton sequencing ENA FIRST PUBLIC:2014 11 29|ENA LAST UPDATE:2018 11 16 RID0023_013.fastq.gz fastq 108193052.0 4221217.0 ena RUN UNIVERSITY OF AMSTERDAM 01 10 2014 14:59:02:785 5 0:25.63 A:29795112;C:21481151;G:26281164;T:30635625;N:0 25       29795112 21481151 26281164 30635625 0 ERX604050 ERS557914 ERA363850 UNIVERSITY OF AMSTERDAM UNIVERSITY OF AMSTERDAM 1 0.78861   0.19966   0.89207   0.49332   22   B   usable mapping rate ion_torrent ion_torrent unknown small_rna unknown bulk unknown unknown   Netherlands 2014-10-01 Adult Adult Undetermined Undetermined
15541 15541 ERR647613 ERX604049 ERS557914 ERP007147 PRJEB7420 Two Sets of Synthetic Spike in Controls for Size Selection and Data Normalisation in small RNA seq ena-STUDY-UNIVERSITY OF AMSTERDAM-01-10-2014-14:02:22:856-622 Other There is an increasing interest in complementing standard RNA seq experiments with small RNA expression data to obtain a comprehensive view of the transcriptome. Two aspects need to be considered when small RNA seq sRNA seq is used. First sRNA seq protocols are size selective and typically optimized for sequencing miRNAs. Second given the dynamic composition of the small RNA fraction a reliable normalization strategy is needed to identify differentially expressed sRNAs. To address both issues we here present two sets of synthetic RNA spike in controls for monitoring size selectivity and for performing normalization. Size spike ins comprising 18 oligoribonucleotides 10–300 nucleotides were designed and tested using controlled modifications to the size selectivity of sRNA seq. As an applied example the loss of RNA molecules <30 nucleotides during small RNA isolation from zebrafish oocytes is demonstrated. Normalization spike ins comprising 19 oligoribonucleotides were designed to use as an external reference for DESeq normalization. Spike in based normalization improved the quantification of predetermined fold changes. Lastly sRNA seq on female and male zebrafish demonstrated that the higher miRNA content in males was correctly preserved post spike in based normalization.       Male 001 SAMEA2796299 UNIVERSITY OF AMSTERDAM Alias:Male 001|ENA checklist:ERC000011|INSDC center alias:UNIVERSITY OF AMSTERDAM|INSDC center name:UNIVERSITY OF AMSTERDAM|INSDC first public:2014 11 29T17:02:28Z|INSDC last update:2014 10 01T14:42:44Z|INSDC status:public|SRA accession:ERS557914|Sample Name:ERS557914|Title:Male ZF|dev stage:Adult|sex:male|strain:ABxTL                 Ion Torrent Proton sequencing ena EXPERIMENT UNIVERSITY OF AMSTERDAM 01 10 2014 14:59:02:785 4 RID0023 1     miRNA-Seq TRANSCRIPTOMIC cDNA SINGLE ION_TORRENT Ion Torrent Proton   ERP007147 Ion Torrent Proton sequencing ENA FIRST PUBLIC:2014 11 29|ENA LAST UPDATE:2018 11 16 RID0023_012.fastq.gz fastq 134807115.0 5232713.0 ena RUN UNIVERSITY OF AMSTERDAM 01 10 2014 14:59:02:785 4 0:25.76 A:37141537;C:27086121;G:32486547;T:38092910;N:0 25       37141537 27086121 32486547 38092910 0 ERX604049 ERS557914 ERA363850 UNIVERSITY OF AMSTERDAM UNIVERSITY OF AMSTERDAM 1 0.78328   0.20061   0.88903   0.51655   32   B   usable mapping rate ion_torrent ion_torrent unknown small_rna unknown bulk unknown unknown   Netherlands 2014-10-01 Adult Adult Undetermined Undetermined
15542 15542 ERR647603 ERX604039 ERS557923 ERP007147 PRJEB7420 Two Sets of Synthetic Spike in Controls for Size Selection and Data Normalisation in small RNA seq ena-STUDY-UNIVERSITY OF AMSTERDAM-01-10-2014-14:02:22:856-622 Other There is an increasing interest in complementing standard RNA seq experiments with small RNA expression data to obtain a comprehensive view of the transcriptome. Two aspects need to be considered when small RNA seq sRNA seq is used. First sRNA seq protocols are size selective and typically optimized for sequencing miRNAs. Second given the dynamic composition of the small RNA fraction a reliable normalization strategy is needed to identify differentially expressed sRNAs. To address both issues we here present two sets of synthetic RNA spike in controls for monitoring size selectivity and for performing normalization. Size spike ins comprising 18 oligoribonucleotides 10–300 nucleotides were designed and tested using controlled modifications to the size selectivity of sRNA seq. As an applied example the loss of RNA molecules <30 nucleotides during small RNA isolation from zebrafish oocytes is demonstrated. Normalization spike ins comprising 19 oligoribonucleotides were designed to use as an external reference for DESeq normalization. Spike in based normalization improved the quantification of predetermined fold changes. Lastly sRNA seq on female and male zebrafish demonstrated that the higher miRNA content in males was correctly preserved post spike in based normalization.       Female 016 SAMEA2796308 UNIVERSITY OF AMSTERDAM Alias:Female 016|ENA checklist:ERC000011|INSDC center alias:UNIVERSITY OF AMSTERDAM|INSDC center name:UNIVERSITY OF AMSTERDAM|INSDC first public:2014 11 29T17:02:28Z|INSDC last update:2014 10 01T14:38:25Z|INSDC status:public|SRA accession:ERS557923|Sample Name:ERS557923|Title:Male vs Female|dev stage:Adult|sex:female|strain:ABxTL                 Ion Torrent Proton sequencing ena EXPERIMENT UNIVERSITY OF AMSTERDAM 01 10 2014 14:37:59:568 8 RID0035 1     miRNA-Seq TRANSCRIPTOMIC cDNA SINGLE ION_TORRENT Ion Torrent Proton   ERP007147 Ion Torrent Proton sequencing ENA FIRST PUBLIC:2014 11 29|ENA LAST UPDATE:2018 11 16 RID0035_010.fastq.gz fastq 186445198.0 6054478.0 ena RUN UNIVERSITY OF AMSTERDAM 01 10 2014 14:37:59:568 8 0:30.79 A:51431122;C:41043403;G:44213690;T:49756983;N:0 30       51431122 41043403 44213690 49756983 0 ERX604039 ERS557923 ERA363846 UNIVERSITY OF AMSTERDAM UNIVERSITY OF AMSTERDAM 1 0.74649   0.44738   0.84254   0.55199   13   B   usable mapping rate ion_torrent ion_torrent unknown small_rna unknown bulk unknown unknown   Netherlands 2014-10-01 Adult Adult Undetermined Undetermined
15543 15543 ERR647602 ERX604038 ERS557922 ERP007147 PRJEB7420 Two Sets of Synthetic Spike in Controls for Size Selection and Data Normalisation in small RNA seq ena-STUDY-UNIVERSITY OF AMSTERDAM-01-10-2014-14:02:22:856-622 Other There is an increasing interest in complementing standard RNA seq experiments with small RNA expression data to obtain a comprehensive view of the transcriptome. Two aspects need to be considered when small RNA seq sRNA seq is used. First sRNA seq protocols are size selective and typically optimized for sequencing miRNAs. Second given the dynamic composition of the small RNA fraction a reliable normalization strategy is needed to identify differentially expressed sRNAs. To address both issues we here present two sets of synthetic RNA spike in controls for monitoring size selectivity and for performing normalization. Size spike ins comprising 18 oligoribonucleotides 10–300 nucleotides were designed and tested using controlled modifications to the size selectivity of sRNA seq. As an applied example the loss of RNA molecules <30 nucleotides during small RNA isolation from zebrafish oocytes is demonstrated. Normalization spike ins comprising 19 oligoribonucleotides were designed to use as an external reference for DESeq normalization. Spike in based normalization improved the quantification of predetermined fold changes. Lastly sRNA seq on female and male zebrafish demonstrated that the higher miRNA content in males was correctly preserved post spike in based normalization.       Female 014 SAMEA2796307 UNIVERSITY OF AMSTERDAM Alias:Female 014|ENA checklist:ERC000011|INSDC center alias:UNIVERSITY OF AMSTERDAM|INSDC center name:UNIVERSITY OF AMSTERDAM|INSDC first public:2014 11 29T17:02:28Z|INSDC last update:2014 10 01T14:38:25Z|INSDC status:public|SRA accession:ERS557922|Sample Name:ERS557922|Title:Male vs Female|dev stage:Adult|sex:female|strain:ABxTL                 Ion Torrent Proton sequencing ena EXPERIMENT UNIVERSITY OF AMSTERDAM 01 10 2014 14:37:59:568 7 RID0035 1     miRNA-Seq TRANSCRIPTOMIC cDNA SINGLE ION_TORRENT Ion Torrent Proton   ERP007147 Ion Torrent Proton sequencing ENA FIRST PUBLIC:2014 11 29|ENA LAST UPDATE:2018 11 16 RID0035_009.fastq.gz fastq 199860614.0 6227225.0 ena RUN UNIVERSITY OF AMSTERDAM 01 10 2014 14:37:59:568 7 0:32.09 A:53999848;C:44698032;G:48251471;T:52911263;N:0 32       53999848 44698032 48251471 52911263 0 ERX604038 ERS557922 ERA363846 UNIVERSITY OF AMSTERDAM UNIVERSITY OF AMSTERDAM 1 0.75262   0.42794   0.83619   0.5624   29   B   usable mapping rate ion_torrent ion_torrent unknown small_rna unknown bulk unknown unknown   Netherlands 2014-10-01 Adult Adult Undetermined Undetermined
15544 15544 ERR647601 ERX604037 ERS557921 ERP007147 PRJEB7420 Two Sets of Synthetic Spike in Controls for Size Selection and Data Normalisation in small RNA seq ena-STUDY-UNIVERSITY OF AMSTERDAM-01-10-2014-14:02:22:856-622 Other There is an increasing interest in complementing standard RNA seq experiments with small RNA expression data to obtain a comprehensive view of the transcriptome. Two aspects need to be considered when small RNA seq sRNA seq is used. First sRNA seq protocols are size selective and typically optimized for sequencing miRNAs. Second given the dynamic composition of the small RNA fraction a reliable normalization strategy is needed to identify differentially expressed sRNAs. To address both issues we here present two sets of synthetic RNA spike in controls for monitoring size selectivity and for performing normalization. Size spike ins comprising 18 oligoribonucleotides 10–300 nucleotides were designed and tested using controlled modifications to the size selectivity of sRNA seq. As an applied example the loss of RNA molecules <30 nucleotides during small RNA isolation from zebrafish oocytes is demonstrated. Normalization spike ins comprising 19 oligoribonucleotides were designed to use as an external reference for DESeq normalization. Spike in based normalization improved the quantification of predetermined fold changes. Lastly sRNA seq on female and male zebrafish demonstrated that the higher miRNA content in males was correctly preserved post spike in based normalization.       Female 008 SAMEA2796306 UNIVERSITY OF AMSTERDAM Alias:Female 008|ENA checklist:ERC000011|INSDC center alias:UNIVERSITY OF AMSTERDAM|INSDC center name:UNIVERSITY OF AMSTERDAM|INSDC first public:2014 11 29T17:02:28Z|INSDC last update:2014 10 01T14:38:25Z|INSDC status:public|SRA accession:ERS557921|Sample Name:ERS557921|Title:Male vs Female|dev stage:Adult|sex:female|strain:ABxTL                 Ion Torrent Proton sequencing ena EXPERIMENT UNIVERSITY OF AMSTERDAM 01 10 2014 14:37:59:568 6 RID0035 1     miRNA-Seq TRANSCRIPTOMIC cDNA SINGLE ION_TORRENT Ion Torrent Proton   ERP007147 Ion Torrent Proton sequencing ENA FIRST PUBLIC:2014 11 29|ENA LAST UPDATE:2018 11 16 RID0035_008.fastq.gz fastq 190251948.0 6082600.0 ena RUN UNIVERSITY OF AMSTERDAM 01 10 2014 14:37:59:568 6 0:31.28 A:50176174;C:43027879;G:47458847;T:49589048;N:0 31       50176174 43027879 47458847 49589048 0 ERX604037 ERS557921 ERA363846 UNIVERSITY OF AMSTERDAM UNIVERSITY OF AMSTERDAM 1 0.72654   0.373   0.82785   0.52436   25   B   usable mapping rate ion_torrent ion_torrent unknown small_rna unknown bulk unknown unknown   Netherlands 2014-10-01 Adult Adult Undetermined Undetermined
15545 15545 ERR647600 ERX604036 ERS557920 ERP007147 PRJEB7420 Two Sets of Synthetic Spike in Controls for Size Selection and Data Normalisation in small RNA seq ena-STUDY-UNIVERSITY OF AMSTERDAM-01-10-2014-14:02:22:856-622 Other There is an increasing interest in complementing standard RNA seq experiments with small RNA expression data to obtain a comprehensive view of the transcriptome. Two aspects need to be considered when small RNA seq sRNA seq is used. First sRNA seq protocols are size selective and typically optimized for sequencing miRNAs. Second given the dynamic composition of the small RNA fraction a reliable normalization strategy is needed to identify differentially expressed sRNAs. To address both issues we here present two sets of synthetic RNA spike in controls for monitoring size selectivity and for performing normalization. Size spike ins comprising 18 oligoribonucleotides 10–300 nucleotides were designed and tested using controlled modifications to the size selectivity of sRNA seq. As an applied example the loss of RNA molecules <30 nucleotides during small RNA isolation from zebrafish oocytes is demonstrated. Normalization spike ins comprising 19 oligoribonucleotides were designed to use as an external reference for DESeq normalization. Spike in based normalization improved the quantification of predetermined fold changes. Lastly sRNA seq on female and male zebrafish demonstrated that the higher miRNA content in males was correctly preserved post spike in based normalization.       Female 007 SAMEA2796305 UNIVERSITY OF AMSTERDAM Alias:Female 007|ENA checklist:ERC000011|INSDC center alias:UNIVERSITY OF AMSTERDAM|INSDC center name:UNIVERSITY OF AMSTERDAM|INSDC first public:2014 11 29T17:02:28Z|INSDC last update:2014 10 01T14:38:24Z|INSDC status:public|SRA accession:ERS557920|Sample Name:ERS557920|Title:Male vs Female|dev stage:Adult|sex:female|strain:ABxTL                 Ion Torrent Proton sequencing ena EXPERIMENT UNIVERSITY OF AMSTERDAM 01 10 2014 14:37:59:568 5 RID0035 1     miRNA-Seq TRANSCRIPTOMIC cDNA SINGLE ION_TORRENT Ion Torrent Proton   ERP007147 Ion Torrent Proton sequencing ENA FIRST PUBLIC:2014 11 29|ENA LAST UPDATE:2018 11 16 RID0035_007.fastq.gz fastq 214503694.0 6990446.0 ena RUN UNIVERSITY OF AMSTERDAM 01 10 2014 14:37:59:568 5 0:30.69 A:59354819;C:47472262;G:51306173;T:56370440;N:0 30       59354819 47472262 51306173 56370440 0 ERX604036 ERS557920 ERA363846 UNIVERSITY OF AMSTERDAM UNIVERSITY OF AMSTERDAM 1 0.74272   0.45587   0.83161   0.53444   22   B   usable mapping rate ion_torrent ion_torrent unknown small_rna unknown bulk unknown unknown   Netherlands 2014-10-01 Adult Adult Undetermined Undetermined
15546 15546 ERR647599 ERX604035 ERS557919 ERP007147 PRJEB7420 Two Sets of Synthetic Spike in Controls for Size Selection and Data Normalisation in small RNA seq ena-STUDY-UNIVERSITY OF AMSTERDAM-01-10-2014-14:02:22:856-622 Other There is an increasing interest in complementing standard RNA seq experiments with small RNA expression data to obtain a comprehensive view of the transcriptome. Two aspects need to be considered when small RNA seq sRNA seq is used. First sRNA seq protocols are size selective and typically optimized for sequencing miRNAs. Second given the dynamic composition of the small RNA fraction a reliable normalization strategy is needed to identify differentially expressed sRNAs. To address both issues we here present two sets of synthetic RNA spike in controls for monitoring size selectivity and for performing normalization. Size spike ins comprising 18 oligoribonucleotides 10–300 nucleotides were designed and tested using controlled modifications to the size selectivity of sRNA seq. As an applied example the loss of RNA molecules <30 nucleotides during small RNA isolation from zebrafish oocytes is demonstrated. Normalization spike ins comprising 19 oligoribonucleotides were designed to use as an external reference for DESeq normalization. Spike in based normalization improved the quantification of predetermined fold changes. Lastly sRNA seq on female and male zebrafish demonstrated that the higher miRNA content in males was correctly preserved post spike in based normalization.       Male 014 SAMEA2796304 UNIVERSITY OF AMSTERDAM Alias:Male 014|ENA checklist:ERC000011|INSDC center alias:UNIVERSITY OF AMSTERDAM|INSDC center name:UNIVERSITY OF AMSTERDAM|INSDC first public:2014 11 29T17:02:28Z|INSDC last update:2014 10 01T14:38:24Z|INSDC status:public|SRA accession:ERS557919|Sample Name:ERS557919|Title:Male vs Female|dev stage:Adult|sex:male|strain:ABxTL                 Ion Torrent Proton sequencing ena EXPERIMENT UNIVERSITY OF AMSTERDAM 01 10 2014 14:37:59:568 4 RID0035 1     miRNA-Seq TRANSCRIPTOMIC cDNA SINGLE ION_TORRENT Ion Torrent Proton   ERP007147 Ion Torrent Proton sequencing ENA FIRST PUBLIC:2014 11 29|ENA LAST UPDATE:2018 11 16 RID0035_005.fastq.gz fastq 235098531.0 7522781.0 ena RUN UNIVERSITY OF AMSTERDAM 01 10 2014 14:37:59:568 4 0:31.25 A:58178492;C:54009752;G:64159987;T:58750300;N:0 31       58178492 54009752 64159987 58750300 0 ERX604035 ERS557919 ERA363846 UNIVERSITY OF AMSTERDAM UNIVERSITY OF AMSTERDAM 1 0.73752   0.19698   0.86665   0.58697   13   B   usable mapping rate ion_torrent ion_torrent unknown small_rna unknown bulk unknown unknown   Netherlands 2014-10-01 Adult Adult Undetermined Undetermined
15547 15547 ERR647598 ERX604034 ERS557918 ERP007147 PRJEB7420 Two Sets of Synthetic Spike in Controls for Size Selection and Data Normalisation in small RNA seq ena-STUDY-UNIVERSITY OF AMSTERDAM-01-10-2014-14:02:22:856-622 Other There is an increasing interest in complementing standard RNA seq experiments with small RNA expression data to obtain a comprehensive view of the transcriptome. Two aspects need to be considered when small RNA seq sRNA seq is used. First sRNA seq protocols are size selective and typically optimized for sequencing miRNAs. Second given the dynamic composition of the small RNA fraction a reliable normalization strategy is needed to identify differentially expressed sRNAs. To address both issues we here present two sets of synthetic RNA spike in controls for monitoring size selectivity and for performing normalization. Size spike ins comprising 18 oligoribonucleotides 10–300 nucleotides were designed and tested using controlled modifications to the size selectivity of sRNA seq. As an applied example the loss of RNA molecules <30 nucleotides during small RNA isolation from zebrafish oocytes is demonstrated. Normalization spike ins comprising 19 oligoribonucleotides were designed to use as an external reference for DESeq normalization. Spike in based normalization improved the quantification of predetermined fold changes. Lastly sRNA seq on female and male zebrafish demonstrated that the higher miRNA content in males was correctly preserved post spike in based normalization.       Male 011 SAMEA2796303 UNIVERSITY OF AMSTERDAM Alias:Male 011|ENA checklist:ERC000011|INSDC center alias:UNIVERSITY OF AMSTERDAM|INSDC center name:UNIVERSITY OF AMSTERDAM|INSDC first public:2014 11 29T17:02:28Z|INSDC last update:2014 10 01T14:38:24Z|INSDC status:public|SRA accession:ERS557918|Sample Name:ERS557918|Title:Male vs Female|dev stage:Adult|sex:male|strain:ABxTL                 Ion Torrent Proton sequencing ena EXPERIMENT UNIVERSITY OF AMSTERDAM 01 10 2014 14:37:59:568 3 RID0035 1     miRNA-Seq TRANSCRIPTOMIC cDNA SINGLE ION_TORRENT Ion Torrent Proton   ERP007147 Ion Torrent Proton sequencing ENA FIRST PUBLIC:2014 11 29|ENA LAST UPDATE:2018 11 16 RID0035_004.fastq.gz fastq 223142261.0 6553993.0 ena RUN UNIVERSITY OF AMSTERDAM 01 10 2014 14:37:59:568 3 0:34.05 A:55488952;C:50941034;G:58123716;T:58588559;N:0 34       55488952 50941034 58123716 58588559 0 ERX604034 ERS557918 ERA363846 UNIVERSITY OF AMSTERDAM UNIVERSITY OF AMSTERDAM 1 0.74686   0.2139   0.86996   0.55375   11   B   usable mapping rate ion_torrent ion_torrent unknown small_rna unknown bulk unknown unknown   Netherlands 2014-10-01 Adult Adult Undetermined Undetermined
15548 15548 ERR647597 ERX604033 ERS557917 ERP007147 PRJEB7420 Two Sets of Synthetic Spike in Controls for Size Selection and Data Normalisation in small RNA seq ena-STUDY-UNIVERSITY OF AMSTERDAM-01-10-2014-14:02:22:856-622 Other There is an increasing interest in complementing standard RNA seq experiments with small RNA expression data to obtain a comprehensive view of the transcriptome. Two aspects need to be considered when small RNA seq sRNA seq is used. First sRNA seq protocols are size selective and typically optimized for sequencing miRNAs. Second given the dynamic composition of the small RNA fraction a reliable normalization strategy is needed to identify differentially expressed sRNAs. To address both issues we here present two sets of synthetic RNA spike in controls for monitoring size selectivity and for performing normalization. Size spike ins comprising 18 oligoribonucleotides 10–300 nucleotides were designed and tested using controlled modifications to the size selectivity of sRNA seq. As an applied example the loss of RNA molecules <30 nucleotides during small RNA isolation from zebrafish oocytes is demonstrated. Normalization spike ins comprising 19 oligoribonucleotides were designed to use as an external reference for DESeq normalization. Spike in based normalization improved the quantification of predetermined fold changes. Lastly sRNA seq on female and male zebrafish demonstrated that the higher miRNA content in males was correctly preserved post spike in based normalization.       Male 006 SAMEA2796302 UNIVERSITY OF AMSTERDAM Alias:Male 006|ENA checklist:ERC000011|INSDC center alias:UNIVERSITY OF AMSTERDAM|INSDC center name:UNIVERSITY OF AMSTERDAM|INSDC first public:2014 11 29T17:02:28Z|INSDC last update:2014 10 01T14:38:24Z|INSDC status:public|SRA accession:ERS557917|Sample Name:ERS557917|Title:Male vs Female|dev stage:Adult|sex:male|strain:ABxTL                 Ion Torrent Proton sequencing ena EXPERIMENT UNIVERSITY OF AMSTERDAM 01 10 2014 14:37:59:568 2 RID0035 1     miRNA-Seq TRANSCRIPTOMIC cDNA SINGLE ION_TORRENT Ion Torrent Proton   ERP007147 Ion Torrent Proton sequencing ENA FIRST PUBLIC:2014 11 29|ENA LAST UPDATE:2018 11 16 RID0035_002.fastq.gz fastq 166401146.0 5656405.0 ena RUN UNIVERSITY OF AMSTERDAM 01 10 2014 14:37:59:568 2 0:29.42 A:41137490;C:38824883;G:44865980;T:41572793;N:0 29       41137490 38824883 44865980 41572793 0 ERX604033 ERS557917 ERA363846 UNIVERSITY OF AMSTERDAM UNIVERSITY OF AMSTERDAM 1 0.69452   0.18245   0.87044   0.58162   11   B   usable mapping rate ion_torrent ion_torrent unknown small_rna unknown bulk unknown unknown   Netherlands 2014-10-01 Adult Adult Undetermined Undetermined
15549 15549 ERR647596 ERX604032 ERS557916 ERP007147 PRJEB7420 Two Sets of Synthetic Spike in Controls for Size Selection and Data Normalisation in small RNA seq ena-STUDY-UNIVERSITY OF AMSTERDAM-01-10-2014-14:02:22:856-622 Other There is an increasing interest in complementing standard RNA seq experiments with small RNA expression data to obtain a comprehensive view of the transcriptome. Two aspects need to be considered when small RNA seq sRNA seq is used. First sRNA seq protocols are size selective and typically optimized for sequencing miRNAs. Second given the dynamic composition of the small RNA fraction a reliable normalization strategy is needed to identify differentially expressed sRNAs. To address both issues we here present two sets of synthetic RNA spike in controls for monitoring size selectivity and for performing normalization. Size spike ins comprising 18 oligoribonucleotides 10–300 nucleotides were designed and tested using controlled modifications to the size selectivity of sRNA seq. As an applied example the loss of RNA molecules <30 nucleotides during small RNA isolation from zebrafish oocytes is demonstrated. Normalization spike ins comprising 19 oligoribonucleotides were designed to use as an external reference for DESeq normalization. Spike in based normalization improved the quantification of predetermined fold changes. Lastly sRNA seq on female and male zebrafish demonstrated that the higher miRNA content in males was correctly preserved post spike in based normalization.       Male 003 SAMEA2796301 UNIVERSITY OF AMSTERDAM Alias:Male 003|ENA checklist:ERC000011|INSDC center alias:UNIVERSITY OF AMSTERDAM|INSDC center name:UNIVERSITY OF AMSTERDAM|INSDC first public:2014 11 29T17:02:28Z|INSDC last update:2014 10 01T14:38:23Z|INSDC status:public|SRA accession:ERS557916|Sample Name:ERS557916|Title:Male vs Female|dev stage:Adult|sex:male|strain:ABxTL                 Ion Torrent Proton sequencing ena EXPERIMENT UNIVERSITY OF AMSTERDAM 01 10 2014 14:37:59:568 1 RID0035 1     miRNA-Seq TRANSCRIPTOMIC cDNA SINGLE ION_TORRENT Ion Torrent Proton   ERP007147 Ion Torrent Proton sequencing ENA FIRST PUBLIC:2014 11 29|ENA LAST UPDATE:2018 11 16 RID0035_001.fastq.gz fastq 167146177.0 6380638.0 ena RUN UNIVERSITY OF AMSTERDAM 01 10 2014 14:37:59:568 1 0:26.20 A:43620211;C:36811253;G:43301042;T:43413671;N:0 26       43620211 36811253 43301042 43413671 0 ERX604032 ERS557916 ERA363846 UNIVERSITY OF AMSTERDAM UNIVERSITY OF AMSTERDAM 1 0.70048   0.18034   0.88075   0.55658   11   B   usable mapping rate ion_torrent ion_torrent unknown small_rna unknown bulk unknown unknown   Netherlands 2014-10-01 Adult Adult Undetermined Undetermined
15550 15550 ERR647595 ERX604031 ERS557915 ERP007147 PRJEB7420 Two Sets of Synthetic Spike in Controls for Size Selection and Data Normalisation in small RNA seq ena-STUDY-UNIVERSITY OF AMSTERDAM-01-10-2014-14:02:22:856-622 Other There is an increasing interest in complementing standard RNA seq experiments with small RNA expression data to obtain a comprehensive view of the transcriptome. Two aspects need to be considered when small RNA seq sRNA seq is used. First sRNA seq protocols are size selective and typically optimized for sequencing miRNAs. Second given the dynamic composition of the small RNA fraction a reliable normalization strategy is needed to identify differentially expressed sRNAs. To address both issues we here present two sets of synthetic RNA spike in controls for monitoring size selectivity and for performing normalization. Size spike ins comprising 18 oligoribonucleotides 10–300 nucleotides were designed and tested using controlled modifications to the size selectivity of sRNA seq. As an applied example the loss of RNA molecules <30 nucleotides during small RNA isolation from zebrafish oocytes is demonstrated. Normalization spike ins comprising 19 oligoribonucleotides were designed to use as an external reference for DESeq normalization. Spike in based normalization improved the quantification of predetermined fold changes. Lastly sRNA seq on female and male zebrafish demonstrated that the higher miRNA content in males was correctly preserved post spike in based normalization.       Egg SAMEA2796300 UNIVERSITY OF AMSTERDAM Alias:Egg|ENA checklist:ERC000011|INSDC center alias:UNIVERSITY OF AMSTERDAM|INSDC center name:UNIVERSITY OF AMSTERDAM|INSDC first public:2014 11 29T17:02:28Z|INSDC last update:2014 10 01T14:29:23Z|INSDC status:public|SRA accession:ERS557915|Sample Name:ERS557915|Title:Zebrafish Egg samples|dev stage:Oocytes                 Ion Torrent Proton sequencing ena EXPERIMENT UNIVERSITY OF AMSTERDAM 01 10 2014 14:25:29:391 1 RID0024 1     miRNA-Seq TRANSCRIPTOMIC cDNA SINGLE ION_TORRENT Ion Torrent Proton   ERP007147 Ion Torrent Proton sequencing ENA FIRST PUBLIC:2014 11 29|ENA LAST UPDATE:2018 11 16 RID0024_016.fastq.gz fastq 6684767188.0 89052017.0 ena RUN UNIVERSITY OF AMSTERDAM 01 10 2014 14:25:29:391 1 0:75.07 A:1523328157;C:1795624453;G:1849675240;T:1516139338;N:0 75       1523328157 1795624453 1849675240 1516139338 0 ERX604031 ERS557915 ERA363845 UNIVERSITY OF AMSTERDAM UNIVERSITY OF AMSTERDAM 1 0.89536   0.15322   0.91421   0.69019   73   B   usable mapping rate ion_torrent ion_torrent unknown small_rna unknown bulk unknown unknown   Netherlands 2014-10-01 Zygote Embryo Oocyte Reproductive System
15551 15551 ERR647594 ERX604030 ERS557914 ERP007147 PRJEB7420 Two Sets of Synthetic Spike in Controls for Size Selection and Data Normalisation in small RNA seq ena-STUDY-UNIVERSITY OF AMSTERDAM-01-10-2014-14:02:22:856-622 Other There is an increasing interest in complementing standard RNA seq experiments with small RNA expression data to obtain a comprehensive view of the transcriptome. Two aspects need to be considered when small RNA seq sRNA seq is used. First sRNA seq protocols are size selective and typically optimized for sequencing miRNAs. Second given the dynamic composition of the small RNA fraction a reliable normalization strategy is needed to identify differentially expressed sRNAs. To address both issues we here present two sets of synthetic RNA spike in controls for monitoring size selectivity and for performing normalization. Size spike ins comprising 18 oligoribonucleotides 10–300 nucleotides were designed and tested using controlled modifications to the size selectivity of sRNA seq. As an applied example the loss of RNA molecules <30 nucleotides during small RNA isolation from zebrafish oocytes is demonstrated. Normalization spike ins comprising 19 oligoribonucleotides were designed to use as an external reference for DESeq normalization. Spike in based normalization improved the quantification of predetermined fold changes. Lastly sRNA seq on female and male zebrafish demonstrated that the higher miRNA content in males was correctly preserved post spike in based normalization.       Male 001 SAMEA2796299 UNIVERSITY OF AMSTERDAM Alias:Male 001|ENA checklist:ERC000011|INSDC center alias:UNIVERSITY OF AMSTERDAM|INSDC center name:UNIVERSITY OF AMSTERDAM|INSDC first public:2014 11 29T17:02:28Z|INSDC last update:2014 10 01T14:42:44Z|INSDC status:public|SRA accession:ERS557914|Sample Name:ERS557914|Title:Male ZF|dev stage:Adult|sex:male|strain:ABxTL                 Ion Torrent Proton sequencing ena EXPERIMENT UNIVERSITY OF AMSTERDAM 01 10 2014 14:02:18:976 6 RID0011 1     miRNA-Seq TRANSCRIPTOMIC cDNA SINGLE ION_TORRENT Ion Torrent Proton   ERP007147 Ion Torrent Proton sequencing ENA FIRST PUBLIC:2014 11 29|ENA LAST UPDATE:2018 11 16 RID0011_015.fastq.gz fastq 273349184.0 9019669.0 ena RUN UNIVERSITY OF AMSTERDAM 01 10 2014 14:02:18:976 6 0:30.31 A:74069549;C:59041449;G:64551451;T:75686735;N:0 30       74069549 59041449 64551451 75686735 0 ERX604030 ERS557914 ERA363842 UNIVERSITY OF AMSTERDAM UNIVERSITY OF AMSTERDAM 1 0.78759   0.26081   0.90989   0.51416   29   B   usable mapping rate ion_torrent ion_torrent unknown small_rna unknown bulk unknown unknown   Netherlands 2014-10-01 Adult Adult Undetermined Undetermined
15552 15552 ERR647589 ERX604025 ERS557914 ERP007147 PRJEB7420 Two Sets of Synthetic Spike in Controls for Size Selection and Data Normalisation in small RNA seq ena-STUDY-UNIVERSITY OF AMSTERDAM-01-10-2014-14:02:22:856-622 Other There is an increasing interest in complementing standard RNA seq experiments with small RNA expression data to obtain a comprehensive view of the transcriptome. Two aspects need to be considered when small RNA seq sRNA seq is used. First sRNA seq protocols are size selective and typically optimized for sequencing miRNAs. Second given the dynamic composition of the small RNA fraction a reliable normalization strategy is needed to identify differentially expressed sRNAs. To address both issues we here present two sets of synthetic RNA spike in controls for monitoring size selectivity and for performing normalization. Size spike ins comprising 18 oligoribonucleotides 10–300 nucleotides were designed and tested using controlled modifications to the size selectivity of sRNA seq. As an applied example the loss of RNA molecules <30 nucleotides during small RNA isolation from zebrafish oocytes is demonstrated. Normalization spike ins comprising 19 oligoribonucleotides were designed to use as an external reference for DESeq normalization. Spike in based normalization improved the quantification of predetermined fold changes. Lastly sRNA seq on female and male zebrafish demonstrated that the higher miRNA content in males was correctly preserved post spike in based normalization.       Male 001 SAMEA2796299 UNIVERSITY OF AMSTERDAM Alias:Male 001|ENA checklist:ERC000011|INSDC center alias:UNIVERSITY OF AMSTERDAM|INSDC center name:UNIVERSITY OF AMSTERDAM|INSDC first public:2014 11 29T17:02:28Z|INSDC last update:2014 10 01T14:42:44Z|INSDC status:public|SRA accession:ERS557914|Sample Name:ERS557914|Title:Male ZF|dev stage:Adult|sex:male|strain:ABxTL                 Ion Torrent Proton sequencing ena EXPERIMENT UNIVERSITY OF AMSTERDAM 01 10 2014 14:02:18:976 1 RID0011 1     miRNA-Seq TRANSCRIPTOMIC cDNA SINGLE ION_TORRENT Ion Torrent Proton   ERP007147 Ion Torrent Proton sequencing ENA FIRST PUBLIC:2014 11 29|ENA LAST UPDATE:2018 11 16 RID0011_010.fastq.gz fastq 54651692.0 2000589.0 ena RUN UNIVERSITY OF AMSTERDAM 01 10 2014 14:02:18:976 1 0:27.32 A:16624629;C:13894132;G:10557585;T:13575346;N:0 27       16624629 13894132 10557585 13575346 0 ERX604025 ERS557914 ERA363842 UNIVERSITY OF AMSTERDAM UNIVERSITY OF AMSTERDAM 1 0.11072   0.02655   0.97749   0.53916   26   B   usable mapping rate ion_torrent ion_torrent unknown small_rna unknown bulk unknown unknown   Netherlands 2014-10-01 Adult Adult Undetermined Undetermined
15553 15553 ERR647590 ERX604026 ERS557914 ERP007147 PRJEB7420 Two Sets of Synthetic Spike in Controls for Size Selection and Data Normalisation in small RNA seq ena-STUDY-UNIVERSITY OF AMSTERDAM-01-10-2014-14:02:22:856-622 Other There is an increasing interest in complementing standard RNA seq experiments with small RNA expression data to obtain a comprehensive view of the transcriptome. Two aspects need to be considered when small RNA seq sRNA seq is used. First sRNA seq protocols are size selective and typically optimized for sequencing miRNAs. Second given the dynamic composition of the small RNA fraction a reliable normalization strategy is needed to identify differentially expressed sRNAs. To address both issues we here present two sets of synthetic RNA spike in controls for monitoring size selectivity and for performing normalization. Size spike ins comprising 18 oligoribonucleotides 10–300 nucleotides were designed and tested using controlled modifications to the size selectivity of sRNA seq. As an applied example the loss of RNA molecules <30 nucleotides during small RNA isolation from zebrafish oocytes is demonstrated. Normalization spike ins comprising 19 oligoribonucleotides were designed to use as an external reference for DESeq normalization. Spike in based normalization improved the quantification of predetermined fold changes. Lastly sRNA seq on female and male zebrafish demonstrated that the higher miRNA content in males was correctly preserved post spike in based normalization.       Male 001 SAMEA2796299 UNIVERSITY OF AMSTERDAM Alias:Male 001|ENA checklist:ERC000011|INSDC center alias:UNIVERSITY OF AMSTERDAM|INSDC center name:UNIVERSITY OF AMSTERDAM|INSDC first public:2014 11 29T17:02:28Z|INSDC last update:2014 10 01T14:42:44Z|INSDC status:public|SRA accession:ERS557914|Sample Name:ERS557914|Title:Male ZF|dev stage:Adult|sex:male|strain:ABxTL                 Ion Torrent Proton sequencing ena EXPERIMENT UNIVERSITY OF AMSTERDAM 01 10 2014 14:02:18:976 2 RID0011 1     miRNA-Seq TRANSCRIPTOMIC cDNA SINGLE ION_TORRENT Ion Torrent Proton   ERP007147 Ion Torrent Proton sequencing ENA FIRST PUBLIC:2014 11 29|ENA LAST UPDATE:2018 11 16 RID0011_011.fastq.gz fastq 47051190.0 1414933.0 ena RUN UNIVERSITY OF AMSTERDAM 01 10 2014 14:02:18:976 2 0:33.25 A:12984359;C:11329853;G:10330928;T:12406050;N:0 33       12984359 11329853 10330928 12406050 0 ERX604026 ERS557914 ERA363842 UNIVERSITY OF AMSTERDAM UNIVERSITY OF AMSTERDAM 1 0.36871   0.10094   0.9483   0.51008   40   B   usable mapping rate ion_torrent ion_torrent unknown small_rna unknown bulk unknown unknown   Netherlands 2014-10-01 Adult Adult Undetermined Undetermined
15554 15554 ERR647592 ERX604028 ERS557914 ERP007147 PRJEB7420 Two Sets of Synthetic Spike in Controls for Size Selection and Data Normalisation in small RNA seq ena-STUDY-UNIVERSITY OF AMSTERDAM-01-10-2014-14:02:22:856-622 Other There is an increasing interest in complementing standard RNA seq experiments with small RNA expression data to obtain a comprehensive view of the transcriptome. Two aspects need to be considered when small RNA seq sRNA seq is used. First sRNA seq protocols are size selective and typically optimized for sequencing miRNAs. Second given the dynamic composition of the small RNA fraction a reliable normalization strategy is needed to identify differentially expressed sRNAs. To address both issues we here present two sets of synthetic RNA spike in controls for monitoring size selectivity and for performing normalization. Size spike ins comprising 18 oligoribonucleotides 10–300 nucleotides were designed and tested using controlled modifications to the size selectivity of sRNA seq. As an applied example the loss of RNA molecules <30 nucleotides during small RNA isolation from zebrafish oocytes is demonstrated. Normalization spike ins comprising 19 oligoribonucleotides were designed to use as an external reference for DESeq normalization. Spike in based normalization improved the quantification of predetermined fold changes. Lastly sRNA seq on female and male zebrafish demonstrated that the higher miRNA content in males was correctly preserved post spike in based normalization.       Male 001 SAMEA2796299 UNIVERSITY OF AMSTERDAM Alias:Male 001|ENA checklist:ERC000011|INSDC center alias:UNIVERSITY OF AMSTERDAM|INSDC center name:UNIVERSITY OF AMSTERDAM|INSDC first public:2014 11 29T17:02:28Z|INSDC last update:2014 10 01T14:42:44Z|INSDC status:public|SRA accession:ERS557914|Sample Name:ERS557914|Title:Male ZF|dev stage:Adult|sex:male|strain:ABxTL                 Ion Torrent Proton sequencing ena EXPERIMENT UNIVERSITY OF AMSTERDAM 01 10 2014 14:02:18:976 4 RID0011 1     miRNA-Seq TRANSCRIPTOMIC cDNA SINGLE ION_TORRENT Ion Torrent Proton   ERP007147 Ion Torrent Proton sequencing ENA FIRST PUBLIC:2014 11 29|ENA LAST UPDATE:2018 11 16 RID0011_013.fastq.gz fastq 394812059.0 13329274.0 ena RUN UNIVERSITY OF AMSTERDAM 01 10 2014 14:02:18:976 4 0:29.62 A:108705027;C:84131955;G:92090437;T:109884640;N:0 29       108705027 84131955 92090437 109884640 0 ERX604028 ERS557914 ERA363842 UNIVERSITY OF AMSTERDAM UNIVERSITY OF AMSTERDAM 1 0.65205   0.21183   0.91977   0.52643   21   B   usable mapping rate ion_torrent ion_torrent unknown small_rna unknown bulk unknown unknown   Netherlands 2014-10-01 Adult Adult Undetermined Undetermined
15555 15555 ERR647593 ERX604029 ERS557914 ERP007147 PRJEB7420 Two Sets of Synthetic Spike in Controls for Size Selection and Data Normalisation in small RNA seq ena-STUDY-UNIVERSITY OF AMSTERDAM-01-10-2014-14:02:22:856-622 Other There is an increasing interest in complementing standard RNA seq experiments with small RNA expression data to obtain a comprehensive view of the transcriptome. Two aspects need to be considered when small RNA seq sRNA seq is used. First sRNA seq protocols are size selective and typically optimized for sequencing miRNAs. Second given the dynamic composition of the small RNA fraction a reliable normalization strategy is needed to identify differentially expressed sRNAs. To address both issues we here present two sets of synthetic RNA spike in controls for monitoring size selectivity and for performing normalization. Size spike ins comprising 18 oligoribonucleotides 10–300 nucleotides were designed and tested using controlled modifications to the size selectivity of sRNA seq. As an applied example the loss of RNA molecules <30 nucleotides during small RNA isolation from zebrafish oocytes is demonstrated. Normalization spike ins comprising 19 oligoribonucleotides were designed to use as an external reference for DESeq normalization. Spike in based normalization improved the quantification of predetermined fold changes. Lastly sRNA seq on female and male zebrafish demonstrated that the higher miRNA content in males was correctly preserved post spike in based normalization.       Male 001 SAMEA2796299 UNIVERSITY OF AMSTERDAM Alias:Male 001|ENA checklist:ERC000011|INSDC center alias:UNIVERSITY OF AMSTERDAM|INSDC center name:UNIVERSITY OF AMSTERDAM|INSDC first public:2014 11 29T17:02:28Z|INSDC last update:2014 10 01T14:42:44Z|INSDC status:public|SRA accession:ERS557914|Sample Name:ERS557914|Title:Male ZF|dev stage:Adult|sex:male|strain:ABxTL                 Ion Torrent Proton sequencing ena EXPERIMENT UNIVERSITY OF AMSTERDAM 01 10 2014 14:02:18:976 5 RID0011 1     miRNA-Seq TRANSCRIPTOMIC cDNA SINGLE ION_TORRENT Ion Torrent Proton   ERP007147 Ion Torrent Proton sequencing ENA FIRST PUBLIC:2014 11 29|ENA LAST UPDATE:2018 11 16 RID0011_014.fastq.gz fastq 143238806.0 4225461.0 ena RUN UNIVERSITY OF AMSTERDAM 01 10 2014 14:02:18:976 5 0:33.90 A:38374880;C:31424768;G:33687670;T:39751488;N:0 33       38374880 31424768 33687670 39751488 0 ERX604029 ERS557914 ERA363842 UNIVERSITY OF AMSTERDAM UNIVERSITY OF AMSTERDAM 1 0.78469   0.26587   0.92178   0.51694   80   B   usable mapping rate ion_torrent ion_torrent unknown small_rna unknown bulk unknown unknown   Netherlands 2014-10-01 Adult Adult Undetermined Undetermined
15556 15556 ERR647591 ERX604027 ERS557914 ERP007147 PRJEB7420 Two Sets of Synthetic Spike in Controls for Size Selection and Data Normalisation in small RNA seq ena-STUDY-UNIVERSITY OF AMSTERDAM-01-10-2014-14:02:22:856-622 Other There is an increasing interest in complementing standard RNA seq experiments with small RNA expression data to obtain a comprehensive view of the transcriptome. Two aspects need to be considered when small RNA seq sRNA seq is used. First sRNA seq protocols are size selective and typically optimized for sequencing miRNAs. Second given the dynamic composition of the small RNA fraction a reliable normalization strategy is needed to identify differentially expressed sRNAs. To address both issues we here present two sets of synthetic RNA spike in controls for monitoring size selectivity and for performing normalization. Size spike ins comprising 18 oligoribonucleotides 10–300 nucleotides were designed and tested using controlled modifications to the size selectivity of sRNA seq. As an applied example the loss of RNA molecules <30 nucleotides during small RNA isolation from zebrafish oocytes is demonstrated. Normalization spike ins comprising 19 oligoribonucleotides were designed to use as an external reference for DESeq normalization. Spike in based normalization improved the quantification of predetermined fold changes. Lastly sRNA seq on female and male zebrafish demonstrated that the higher miRNA content in males was correctly preserved post spike in based normalization.       Male 001 SAMEA2796299 UNIVERSITY OF AMSTERDAM Alias:Male 001|ENA checklist:ERC000011|INSDC center alias:UNIVERSITY OF AMSTERDAM|INSDC center name:UNIVERSITY OF AMSTERDAM|INSDC first public:2014 11 29T17:02:28Z|INSDC last update:2014 10 01T14:42:44Z|INSDC status:public|SRA accession:ERS557914|Sample Name:ERS557914|Title:Male ZF|dev stage:Adult|sex:male|strain:ABxTL                 Ion Torrent Proton sequencing ena EXPERIMENT UNIVERSITY OF AMSTERDAM 01 10 2014 14:02:18:976 3 RID0011 1     miRNA-Seq TRANSCRIPTOMIC cDNA SINGLE ION_TORRENT Ion Torrent Proton   ERP007147 Ion Torrent Proton sequencing ENA FIRST PUBLIC:2014 11 29|ENA LAST UPDATE:2018 11 16 RID0011_012.fastq.gz fastq 55541174.0 1649692.0 ena RUN UNIVERSITY OF AMSTERDAM 01 10 2014 14:02:18:976 3 0:33.67 A:16076639;C:14164415;G:11139997;T:14160123;N:0 33       16076639 14164415 11139997 14160123 0 ERX604027 ERS557914 ERA363842 UNIVERSITY OF AMSTERDAM UNIVERSITY OF AMSTERDAM 1 0.16692   0.04528   0.96384   0.52823   17   B   usable mapping rate ion_torrent ion_torrent unknown small_rna unknown bulk unknown unknown   Netherlands 2014-10-01 Adult Adult Undetermined Undetermined
28470 28470 SRR26253203 SRX21963295 SRS19039869 SRP464138 PRJNA1022587 Danio rerio Raw sequence reads PRJNA1022587 Whole Genome Sequencing In teleost type I IFNs are categorized into 2 subgroups containing one or two pairs of disulphide bond. However their functional differences have not been fully unveiled. It has been shown that IFN1 can be induced by viruses and trigger strong antiviral response in inducing hundreds of IFN stimulated genes conferring cell resistance to viruses. However the antiviral functions of IFN4 have been debated. To investigate the genes modulated by IFN1 and IFN4 ZF4 cells were stimulated with recombinant IFN1 and IFN4 and were performed transcriptome analysis of the small RNA.         Small RNA IFN4 2   strain:not collected|age:not collected|collection date:2023 08 20|geo loc name:not collected|sex:not collected|tissue:not collected|cell line:ZF4|replicate:biological replicate 22|BioSampleModel:Model organism or animal                 Small RNAseq of zebrafish Small RNA IFND2 Small RNA IFND2 small RNA seq of zebrafish     miRNA-Seq TRANSCRIPTOMIC PCR SINGLE ILLUMINA Illumina NovaSeq 6000   SRP464138     IFN4-2.deadaptor.fq.gz fastq 278362309.0 11056139.0 IFN4 2.deadaptor.fq.gz 0:25.18 A:71944968;C:54562141;G:73333701;T:78511075;N:10424 25       71944968 54562141 73333701 78511075 10424 SRX21963295 SRS19039869 SRA1724093 Shanghai Ocean University|College of Fisheries and Life Science Shanghai Ocean University 1 0.93133   0.07401   0.96181   0.74558   31   B   usable mapping rate illumina novaseq_era unknown random_priming unknown bulk unknown unknown   China 2023-10-02 Undetermined Undetermined Cell Line Cell Line
28471 28471 SRR26253204 SRX21963294 SRS19039868 SRP464138 PRJNA1022587 Danio rerio Raw sequence reads PRJNA1022587 Whole Genome Sequencing In teleost type I IFNs are categorized into 2 subgroups containing one or two pairs of disulphide bond. However their functional differences have not been fully unveiled. It has been shown that IFN1 can be induced by viruses and trigger strong antiviral response in inducing hundreds of IFN stimulated genes conferring cell resistance to viruses. However the antiviral functions of IFN4 have been debated. To investigate the genes modulated by IFN1 and IFN4 ZF4 cells were stimulated with recombinant IFN1 and IFN4 and were performed transcriptome analysis of the small RNA.         Small RNA IFN4 1   strain:not collected|age:not collected|collection date:2023 08 20|geo loc name:not collected|sex:not collected|tissue:not collected|cell line:ZF4|replicate:biological replicate 21|BioSampleModel:Model organism or animal                 Small RNAseq of zebrafish Small RNA IFND1 Small RNA IFND1 small RNA seq of zebrafish     miRNA-Seq TRANSCRIPTOMIC PCR SINGLE ILLUMINA Illumina NovaSeq 6000   SRP464138     IFN4-1.deadaptor.fq.gz fastq 289362334.0 11048590.0 IFN4 1.deadaptor.fq.gz 0:26.19 A:73933649;C:59386039;G:76847534;T:79184583;N:10529 26       73933649 59386039 76847534 79184583 10529 SRX21963294 SRS19039868 SRA1724093 Shanghai Ocean University|College of Fisheries and Life Science Shanghai Ocean University 1 0.91683   0.07099   0.96132   0.64882   25   B   usable mapping rate illumina novaseq_era unknown random_priming unknown bulk unknown unknown   China 2023-10-02 Undetermined Undetermined Cell Line Cell Line
28472 28472 SRR26253205 SRX21963293 SRS19039867 SRP464138 PRJNA1022587 Danio rerio Raw sequence reads PRJNA1022587 Whole Genome Sequencing In teleost type I IFNs are categorized into 2 subgroups containing one or two pairs of disulphide bond. However their functional differences have not been fully unveiled. It has been shown that IFN1 can be induced by viruses and trigger strong antiviral response in inducing hundreds of IFN stimulated genes conferring cell resistance to viruses. However the antiviral functions of IFN4 have been debated. To investigate the genes modulated by IFN1 and IFN4 ZF4 cells were stimulated with recombinant IFN1 and IFN4 and were performed transcriptome analysis of the small RNA.         Small RNA IFN1 4   strain:not collected|age:not collected|collection date:2023 08 20|geo loc name:not collected|sex:not collected|tissue:not collected|cell line:ZF4|replicate:biological replicate 20|BioSampleModel:Model organism or animal                 Small RNAseq of zebrafish Small RNA IFNA4 Small RNA IFNA4 small RNA seq of zebrafish     miRNA-Seq TRANSCRIPTOMIC PCR SINGLE ILLUMINA Illumina NovaSeq 6000   SRP464138     IFN1-4.deadaptor.fq.gz fastq 270529395.0 11070360.0 IFN1 4.deadaptor.fq.gz 0:24.44 A:69759888;C:54924667;G:71358091;T:74470720;N:16029 24       69759888 54924667 71358091 74470720 16029 SRX21963293 SRS19039867 SRA1724093 Shanghai Ocean University|College of Fisheries and Life Science Shanghai Ocean University 1 0.88469   0.06696   0.96664   0.74853   22   B   usable mapping rate illumina novaseq_era unknown random_priming unknown bulk unknown unknown   China 2023-10-02 Undetermined Undetermined Cell Line Cell Line
28473 28473 SRR26253206 SRX21963292 SRS19039866 SRP464138 PRJNA1022587 Danio rerio Raw sequence reads PRJNA1022587 Whole Genome Sequencing In teleost type I IFNs are categorized into 2 subgroups containing one or two pairs of disulphide bond. However their functional differences have not been fully unveiled. It has been shown that IFN1 can be induced by viruses and trigger strong antiviral response in inducing hundreds of IFN stimulated genes conferring cell resistance to viruses. However the antiviral functions of IFN4 have been debated. To investigate the genes modulated by IFN1 and IFN4 ZF4 cells were stimulated with recombinant IFN1 and IFN4 and were performed transcriptome analysis of the small RNA.         Small RNA IFN1 3   strain:not collected|age:not collected|collection date:2023 08 20|geo loc name:not collected|sex:not collected|tissue:not collected|cell line:ZF4|replicate:biological replicate 19|BioSampleModel:Model organism or animal                 Small RNAseq of zebrafish Small RNA IFNA3 Small RNA IFNA3 small RNA seq of zebrafish     miRNA-Seq TRANSCRIPTOMIC PCR SINGLE ILLUMINA Illumina NovaSeq 6000   SRP464138     IFN1-3.deadaptor.fq.gz fastq 353291957.0 11740560.0 IFN1 3.deadaptor.fq.gz 0:30.09 A:89026549;C:76490337;G:94719420;T:93036955;N:18696 30       89026549 76490337 94719420 93036955 18696 SRX21963292 SRS19039866 SRA1724093 Shanghai Ocean University|College of Fisheries and Life Science Shanghai Ocean University 1 0.83151   0.07199   0.96471   0.71641   22   B   usable mapping rate illumina novaseq_era unknown random_priming unknown bulk unknown unknown   China 2023-10-02 Undetermined Undetermined Cell Line Cell Line
28474 28474 SRR26253207 SRX21963291 SRS19039865 SRP464138 PRJNA1022587 Danio rerio Raw sequence reads PRJNA1022587 Whole Genome Sequencing In teleost type I IFNs are categorized into 2 subgroups containing one or two pairs of disulphide bond. However their functional differences have not been fully unveiled. It has been shown that IFN1 can be induced by viruses and trigger strong antiviral response in inducing hundreds of IFN stimulated genes conferring cell resistance to viruses. However the antiviral functions of IFN4 have been debated. To investigate the genes modulated by IFN1 and IFN4 ZF4 cells were stimulated with recombinant IFN1 and IFN4 and were performed transcriptome analysis of the small RNA.         Small RNA IFN1 2   strain:not collected|age:not collected|collection date:2023 08 20|geo loc name:not collected|sex:not collected|tissue:not collected|cell line:ZF4|replicate:biological replicate 18|BioSampleModel:Model organism or animal                 Small RNAseq of zebrafish Small RNA IFNA2 Small RNA IFNA2 small RNA seq of zebrafish     miRNA-Seq TRANSCRIPTOMIC PCR SINGLE ILLUMINA Illumina NovaSeq 6000   SRP464138     IFN1-2.deadaptor.fq.gz fastq 257949577.0 11213774.0 IFN1 2.deadaptor.fq.gz 0:23.00 A:67371681;C:51128621;G:67605590;T:71826657;N:17028 23       67371681 51128621 67605590 71826657 17028 SRX21963291 SRS19039865 SRA1724093 Shanghai Ocean University|College of Fisheries and Life Science Shanghai Ocean University 1 0.91396   0.06536   0.97197   0.76269   23   B   usable mapping rate illumina novaseq_era unknown random_priming unknown bulk unknown unknown   China 2023-10-02 Undetermined Undetermined Cell Line Cell Line
28475 28475 SRR26253208 SRX21963290 SRS19039864 SRP464138 PRJNA1022587 Danio rerio Raw sequence reads PRJNA1022587 Whole Genome Sequencing In teleost type I IFNs are categorized into 2 subgroups containing one or two pairs of disulphide bond. However their functional differences have not been fully unveiled. It has been shown that IFN1 can be induced by viruses and trigger strong antiviral response in inducing hundreds of IFN stimulated genes conferring cell resistance to viruses. However the antiviral functions of IFN4 have been debated. To investigate the genes modulated by IFN1 and IFN4 ZF4 cells were stimulated with recombinant IFN1 and IFN4 and were performed transcriptome analysis of the small RNA.         Small RNA IFN1 1   strain:not collected|age:not collected|collection date:2023 08 20|geo loc name:not collected|sex:not collected|tissue:not collected|cell line:ZF4|replicate:biological replicate 17|BioSampleModel:Model organism or animal                 Small RNAseq of zebrafish Small RNA IFNA1 Small RNA IFNA1 small RNA seq of zebrafish     miRNA-Seq TRANSCRIPTOMIC PCR SINGLE ILLUMINA Illumina NovaSeq 6000   SRP464138     IFN1-1.deadaptor.fq.gz fastq 329358383.0 11799176.0 IFN1 1.deadaptor.fq.gz 0:27.91 A:84093681;C:68961271;G:86502739;T:89779605;N:21087 27       84093681 68961271 86502739 89779605 21087 SRX21963290 SRS19039864 SRA1724093 Shanghai Ocean University|College of Fisheries and Life Science Shanghai Ocean University 1 0.92116   0.07483   0.96796   0.75179   22   B   usable mapping rate illumina novaseq_era unknown random_priming unknown bulk unknown unknown   China 2023-10-02 Undetermined Undetermined Cell Line Cell Line
28476 28476 SRR26253209 SRX21963289 SRS19039863 SRP464138 PRJNA1022587 Danio rerio Raw sequence reads PRJNA1022587 Whole Genome Sequencing In teleost type I IFNs are categorized into 2 subgroups containing one or two pairs of disulphide bond. However their functional differences have not been fully unveiled. It has been shown that IFN1 can be induced by viruses and trigger strong antiviral response in inducing hundreds of IFN stimulated genes conferring cell resistance to viruses. However the antiviral functions of IFN4 have been debated. To investigate the genes modulated by IFN1 and IFN4 ZF4 cells were stimulated with recombinant IFN1 and IFN4 and were performed transcriptome analysis of the small RNA.         Small RNA Control 4   strain:not collected|age:not collected|collection date:2023 08 20|geo loc name:not collected|sex:not collected|tissue:not collected|cell line:ZF4|replicate:biological replicate 16|BioSampleModel:Model organism or animal                 Small RNAseq of zebrafish Small RNA C4 Small RNA C4 small RNA seq of zebrafish     miRNA-Seq TRANSCRIPTOMIC PCR SINGLE ILLUMINA Illumina NovaSeq 6000   SRP464138     Control-4.deadaptor.fq.gz fastq 251577736.0 10951659.0 Control 4.deadaptor.fq.gz 0:22.97 A:65382449;C:50041232;G:66038625;T:70106464;N:8966 22       65382449 50041232 66038625 70106464 8966 SRX21963289 SRS19039863 SRA1724093 Shanghai Ocean University|College of Fisheries and Life Science Shanghai Ocean University 1 0.89744   0.06821   0.96607   0.63829   22   B   usable mapping rate illumina novaseq_era unknown random_priming unknown bulk unknown unknown   China 2023-10-02 Undetermined Undetermined Cell Line Cell Line
28477 28477 SRR26253210 SRX21963288 SRS19039862 SRP464138 PRJNA1022587 Danio rerio Raw sequence reads PRJNA1022587 Whole Genome Sequencing In teleost type I IFNs are categorized into 2 subgroups containing one or two pairs of disulphide bond. However their functional differences have not been fully unveiled. It has been shown that IFN1 can be induced by viruses and trigger strong antiviral response in inducing hundreds of IFN stimulated genes conferring cell resistance to viruses. However the antiviral functions of IFN4 have been debated. To investigate the genes modulated by IFN1 and IFN4 ZF4 cells were stimulated with recombinant IFN1 and IFN4 and were performed transcriptome analysis of the small RNA.         Small RNA Control 3   strain:not collected|age:not collected|collection date:2023 08 20|geo loc name:not collected|sex:not collected|tissue:not collected|cell line:ZF4|replicate:biological replicate 15|BioSampleModel:Model organism or animal                 Small RNAseq of zebrafish Small RNA C3 Small RNA C3 small RNA seq of zebrafish     miRNA-Seq TRANSCRIPTOMIC PCR SINGLE ILLUMINA Illumina NovaSeq 6000   SRP464138     Control-3.deadaptor.fq.gz fastq 252164979.0 11004167.0 Control 3.deadaptor.fq.gz 0:22.92 A:65850899;C:48466665;G:66708038;T:71130786;N:8591 22       65850899 48466665 66708038 71130786 8591 SRX21963288 SRS19039862 SRA1724093 Shanghai Ocean University|College of Fisheries and Life Science Shanghai Ocean University 1 0.91567   0.06804   0.96278   0.75579   22   B   usable mapping rate illumina novaseq_era unknown random_priming unknown bulk unknown unknown   China 2023-10-02 Undetermined Undetermined Cell Line Cell Line
28478 28478 SRR26253211 SRX21963287 SRS19039861 SRP464138 PRJNA1022587 Danio rerio Raw sequence reads PRJNA1022587 Whole Genome Sequencing In teleost type I IFNs are categorized into 2 subgroups containing one or two pairs of disulphide bond. However their functional differences have not been fully unveiled. It has been shown that IFN1 can be induced by viruses and trigger strong antiviral response in inducing hundreds of IFN stimulated genes conferring cell resistance to viruses. However the antiviral functions of IFN4 have been debated. To investigate the genes modulated by IFN1 and IFN4 ZF4 cells were stimulated with recombinant IFN1 and IFN4 and were performed transcriptome analysis of the small RNA.         Small RNA IFN4 4   strain:not collected|age:not collected|collection date:2023 08 20|geo loc name:not collected|sex:not collected|tissue:not collected|cell line:ZF4|replicate:biological replicate 24|BioSampleModel:Model organism or animal                 Small RNAseq of zebrafish Small RNA IFND4 Small RNA IFND4 small RNA seq of zebrafish     miRNA-Seq TRANSCRIPTOMIC PCR SINGLE ILLUMINA Illumina NovaSeq 6000   SRP464138     IFN4-4.deadaptor.fq.gz fastq 291708053.0 11503883.0 IFN4 4.deadaptor.fq.gz 0:25.36 A:74079231;C:61368459;G:76898572;T:79351225;N:10566 25       74079231 61368459 76898572 79351225 10566 SRX21963287 SRS19039861 SRA1724093 Shanghai Ocean University|College of Fisheries and Life Science Shanghai Ocean University 1 0.88888   0.0633   0.97305   0.74862   23   B   usable mapping rate illumina novaseq_era unknown random_priming unknown bulk unknown unknown   China 2023-10-02 Undetermined Undetermined Cell Line Cell Line
28479 28479 SRR26253212 SRX21963286 SRS19039860 SRP464138 PRJNA1022587 Danio rerio Raw sequence reads PRJNA1022587 Whole Genome Sequencing In teleost type I IFNs are categorized into 2 subgroups containing one or two pairs of disulphide bond. However their functional differences have not been fully unveiled. It has been shown that IFN1 can be induced by viruses and trigger strong antiviral response in inducing hundreds of IFN stimulated genes conferring cell resistance to viruses. However the antiviral functions of IFN4 have been debated. To investigate the genes modulated by IFN1 and IFN4 ZF4 cells were stimulated with recombinant IFN1 and IFN4 and were performed transcriptome analysis of the small RNA.         Small RNA IFN4 3   strain:not collected|age:not collected|collection date:2023 08 20|geo loc name:not collected|sex:not collected|tissue:not collected|cell line:ZF4|replicate:biological replicate 23|BioSampleModel:Model organism or animal                 Small RNAseq of zebrafish Small RNA IFND3 Small RNA IFND3 small RNA seq of zebrafish     miRNA-Seq TRANSCRIPTOMIC PCR SINGLE ILLUMINA Illumina NovaSeq 6000   SRP464138     IFN4-3.deadaptor.fq.gz fastq 349995495.0 11885662.0 IFN4 3.deadaptor.fq.gz 0:29.45 A:87443709;C:76373108;G:92741296;T:93425866;N:11516 29       87443709 76373108 92741296 93425866 11516 SRX21963286 SRS19039860 SRA1724093 Shanghai Ocean University|College of Fisheries and Life Science Shanghai Ocean University 1 0.92619   0.06843   0.9709   0.74977   73   B   usable mapping rate illumina novaseq_era unknown random_priming unknown bulk unknown unknown   China 2023-10-02 Undetermined Undetermined Cell Line Cell Line
28480 28480 SRR26253213 SRX21963285 SRS19039859 SRP464138 PRJNA1022587 Danio rerio Raw sequence reads PRJNA1022587 Whole Genome Sequencing In teleost type I IFNs are categorized into 2 subgroups containing one or two pairs of disulphide bond. However their functional differences have not been fully unveiled. It has been shown that IFN1 can be induced by viruses and trigger strong antiviral response in inducing hundreds of IFN stimulated genes conferring cell resistance to viruses. However the antiviral functions of IFN4 have been debated. To investigate the genes modulated by IFN1 and IFN4 ZF4 cells were stimulated with recombinant IFN1 and IFN4 and were performed transcriptome analysis of the small RNA.         Small RNA Control 2   strain:not collected|age:not collected|collection date:2023 08 20|geo loc name:not collected|sex:not collected|tissue:not collected|cell line:ZF4|replicate:biological replicate 14|BioSampleModel:Model organism or animal                 Small RNAseq of zebrafish Small RNA C2 Small RNA C2 small RNA seq of zebrafish     miRNA-Seq TRANSCRIPTOMIC PCR SINGLE ILLUMINA Illumina NovaSeq 6000   SRP464138     Control-2.deadaptor.fq.gz fastq 254163676.0 10744164.0 Control 2.deadaptor.fq.gz 0:23.66 A:66003417;C:49309846;G:67463940;T:71377644;N:8829 23       66003417 49309846 67463940 71377644 8829 SRX21963285 SRS19039859 SRA1724093 Shanghai Ocean University|College of Fisheries and Life Science Shanghai Ocean University 1 0.93068   0.06626   0.96441   0.7492   22   B   usable mapping rate illumina novaseq_era unknown random_priming unknown bulk unknown unknown   China 2023-10-02 Undetermined Undetermined Cell Line Cell Line
28481 28481 SRR26253214 SRX21963284 SRS19039858 SRP464138 PRJNA1022587 Danio rerio Raw sequence reads PRJNA1022587 Whole Genome Sequencing In teleost type I IFNs are categorized into 2 subgroups containing one or two pairs of disulphide bond. However their functional differences have not been fully unveiled. It has been shown that IFN1 can be induced by viruses and trigger strong antiviral response in inducing hundreds of IFN stimulated genes conferring cell resistance to viruses. However the antiviral functions of IFN4 have been debated. To investigate the genes modulated by IFN1 and IFN4 ZF4 cells were stimulated with recombinant IFN1 and IFN4 and were performed transcriptome analysis of the small RNA.         Small RNA Control 1   strain:not collected|age:not collected|collection date:2023 08 20|geo loc name:not collected|sex:not collected|tissue:not collected|cell line:ZF4|replicate:biological replicate 13|BioSampleModel:Model organism or animal                 Small RNAseq of zebrafish Small RNA C1 Small RNA C1 small RNA seq of zebrafish     miRNA-Seq TRANSCRIPTOMIC PCR SINGLE ILLUMINA Illumina NovaSeq 6000   SRP464138     Control-1.deadaptor.fq.gz fastq 267090429.0 11027241.0 Control 1.deadaptor.fq.gz 0:24.22 A:70162511;C:50705437;G:69872659;T:76339682;N:10140 24       70162511 50705437 69872659 76339682 10140 SRX21963284 SRS19039858 SRA1724093 Shanghai Ocean University|College of Fisheries and Life Science Shanghai Ocean University 1 0.93267   0.07189   0.96735   0.75447   21   B   usable mapping rate illumina novaseq_era unknown random_priming unknown bulk unknown unknown   China 2023-10-02 Undetermined Undetermined Cell Line Cell Line
32541 32541 SRR29303120 SRX24820197 SRS21534299 SRP512097 PRJNA1120592 Light induce Zebrafish larvae PRJNA1120592 Other Light induce Zebrafish larvae miRNA         L6H 1 miRNA   breed:zebrafish|cultivar:not applicable|ecotype:AB|age:5 dpf|dev stage:larvae|collection date:2020 10 10|geo loc name:China: Lanzhou|sex:not determined|tissue:whole body|BioSampleModel:Model organism or animal                 miRNA seq of zebrafish larvae L6H 1 L6H 1 miRNA seq     miRNA-Seq TRANSCRIPTOMIC RANDOM PCR SINGLE ILLUMINA Illumina HiSeq 3000   SRP512097     L6H_1.fq.gz fastq 619966455.0 12156205.0 L6H 1.fq.gz 0:51 A:143411079;C:162001488;G:168382605;T:146007422;N:163861 51       143411079 162001488 168382605 146007422 163861 SRX24820197 SRS21534299 SRA1890590 Lanzhou University|College of Life Science Lanzhou University 1 0.01303   0.00337   0.99472   0.5628   51   B   usable mapping rate illumina hiseq_era unknown random_priming unknown bulk unknown unknown   China 2024-06-06 Larval Larval Trunk Surface Structure
32542 32542 SRR29303121 SRX24820196 SRS21534298 SRP512097 PRJNA1120592 Light induce Zebrafish larvae PRJNA1120592 Other Light induce Zebrafish larvae miRNA         L3H 3 miRNA   breed:zebrafish|cultivar:not applicable|ecotype:AB|age:5 dpf|dev stage:larvae|collection date:2020 10 09|geo loc name:China: Lanzhou|sex:not determined|tissue:whole body|BioSampleModel:Model organism or animal                 miRNA seq of zebrafish larvae L3H 3 L3H 3 miRNA seq     miRNA-Seq TRANSCRIPTOMIC RANDOM PCR SINGLE ILLUMINA Illumina HiSeq 3000   SRP512097     L3H_3.fq.gz fastq 738125703.0 14473053.0 L3H 3.fq.gz 0:51 A:176614297;C:190760467;G:197157923;T:173400138;N:192878 51       176614297 190760467 197157923 173400138 192878 SRX24820196 SRS21534298 SRA1890590 Lanzhou University|College of Life Science Lanzhou University 1 0.01163   0.00356   0.99379   0.55469   51   T   under 1.2% mapping rate illumina hiseq_era unknown random_priming unknown bulk unknown unknown   China 2024-06-06 Larval Larval Trunk Surface Structure
32543 32543 SRR29303122 SRX24820195 SRS21534297 SRP512097 PRJNA1120592 Light induce Zebrafish larvae PRJNA1120592 Other Light induce Zebrafish larvae miRNA         L3H 2 miRNA   breed:zebrafish|cultivar:not applicable|ecotype:AB|age:5 dpf|dev stage:larvae|collection date:2020 10 08|geo loc name:China: Lanzhou|sex:not determined|tissue:whole body|BioSampleModel:Model organism or animal                 miRNA seq of zebrafish larvae L3H 2 L3H 2 miRNA seq     miRNA-Seq TRANSCRIPTOMIC RANDOM PCR SINGLE ILLUMINA Illumina HiSeq 3000   SRP512097     L3H_2.fq.gz fastq 489154821.0 9591271.0 L3H 2.fq.gz 0:51 A:109695797;C:132121151;G:127787221;T:119539033;N:11619 51       109695797 132121151 127787221 119539033 11619 SRX24820195 SRS21534297 SRA1890590 Lanzhou University|College of Life Science Lanzhou University 1 0.05359   0.01132   0.98924   0.55364   51   B   usable mapping rate illumina hiseq_era unknown random_priming unknown bulk unknown unknown   China 2024-06-06 Larval Larval Trunk Surface Structure
32544 32544 SRR29303123 SRX24820194 SRS21534296 SRP512097 PRJNA1120592 Light induce Zebrafish larvae PRJNA1120592 Other Light induce Zebrafish larvae miRNA         L3H 1 miRNA   breed:zebrafish|cultivar:not applicable|ecotype:AB|age:5 dpf|dev stage:larvae|collection date:2020 10 07|geo loc name:China: Lanzhou|sex:not determined|tissue:whole body|BioSampleModel:Model organism or animal                 miRNA seq of zebrafish larvae L3H 1 L3H 1 miRNA seq     miRNA-Seq TRANSCRIPTOMIC RANDOM PCR SINGLE ILLUMINA Illumina HiSeq 3000   SRP512097     L3H_1.fq.gz fastq 551502015.0 10813765.0 L3H 1.fq.gz 0:51 A:130437158;C:141969110;G:146479841;T:132605147;N:10759 51       130437158 141969110 146479841 132605147 10759 SRX24820194 SRS21534296 SRA1890590 Lanzhou University|College of Life Science Lanzhou University 1 0.13403   0.01834   0.98735   0.54849   51   B   usable mapping rate illumina hiseq_era unknown random_priming unknown bulk unknown unknown   China 2024-06-06 Larval Larval Trunk Surface Structure
32545 32545 SRR29303124 SRX24820193 SRS21534295 SRP512097 PRJNA1120592 Light induce Zebrafish larvae PRJNA1120592 Other Light induce Zebrafish larvae miRNA         L1H 3 miRNA   breed:zebrafish|cultivar:not applicable|ecotype:AB|age:5 dpf|dev stage:larvae|collection date:2020 10 06|geo loc name:China: Lanzhou|sex:not determined|tissue:whole body|BioSampleModel:Model organism or animal                 miRNA seq of zebrafish larvae L1H 3 L1H 3 miRNA seq     miRNA-Seq TRANSCRIPTOMIC RANDOM PCR SINGLE ILLUMINA Illumina HiSeq 3000   SRP512097     L1H_3.fq.gz fastq 524533827.0 10284977.0 L1H 3.fq.gz 0:51 A:118598837;C:136729611;G:142993590;T:126201873;N:9916 51       118598837 136729611 142993590 126201873 9916 SRX24820193 SRS21534295 SRA1890590 Lanzhou University|College of Life Science Lanzhou University 1 0.00829   0.00187   0.99472   0.54701   51   T   under 1.2% mapping rate illumina hiseq_era unknown random_priming unknown bulk unknown unknown   China 2024-06-06 Larval Larval Trunk Surface Structure
32546 32546 SRR29303125 SRX24820192 SRS21534294 SRP512097 PRJNA1120592 Light induce Zebrafish larvae PRJNA1120592 Other Light induce Zebrafish larvae miRNA         L1H 2 miRNA   breed:zebrafish|cultivar:not applicable|ecotype:AB|age:5 dpf|dev stage:larvae|collection date:2020 10 05|geo loc name:China: Lanzhou|sex:not determined|tissue:whole body|BioSampleModel:Model organism or animal                 miRNA seq of zebrafish larvae L1H 2 L1H 2 miRNA seq     miRNA-Seq TRANSCRIPTOMIC RANDOM PCR SINGLE ILLUMINA Illumina HiSeq 3000   SRP512097     L1H_2.fq.gz fastq 541697163.0 10621513.0 L1H 2.fq.gz 0:51 A:126751483;C:135818134;G:141122995;T:137953543;N:51008 51       126751483 135818134 141122995 137953543 51008 SRX24820192 SRS21534294 SRA1890590 Lanzhou University|College of Life Science Lanzhou University 1 0.33438   0.08101   0.98238   0.53337   51   B   usable mapping rate illumina hiseq_era unknown random_priming unknown bulk unknown unknown   China 2024-06-06 Larval Larval Trunk Surface Structure
32547 32547 SRR29303126 SRX24820191 SRS21534293 SRP512097 PRJNA1120592 Light induce Zebrafish larvae PRJNA1120592 Other Light induce Zebrafish larvae miRNA         L1H 1 miRNA   breed:zebrafish|cultivar:not applicable|ecotype:AB|age:5 dpf|dev stage:larvae|collection date:2020 10 04|geo loc name:China: Lanzhou|sex:not determined|tissue:whole body|BioSampleModel:Model organism or animal                 miRNA seq of zebrafish larvae L1H 1 L1H 1 miRNA seq     miRNA-Seq TRANSCRIPTOMIC RANDOM PCR SINGLE ILLUMINA Illumina HiSeq 3000   SRP512097     L1H_1.fq.gz fastq 930096282.0 18237182.0 L1H 1.fq.gz 0:51 A:212568445;C:243497911;G:260145458;T:213851032;N:33436 51       212568445 243497911 260145458 213851032 33436 SRX24820191 SRS21534293 SRA1890590 Lanzhou University|College of Life Science Lanzhou University 1 0.64143   0.02683   0.98683   0.5342   51   B   usable mapping rate illumina hiseq_era unknown random_priming unknown bulk unknown unknown   China 2024-06-06 Larval Larval Trunk Surface Structure
32548 32548 SRR29303127 SRX24820190 SRS21534292 SRP512097 PRJNA1120592 Light induce Zebrafish larvae PRJNA1120592 Other Light induce Zebrafish larvae miRNA         DD 3 miRNA   breed:zebrafish|cultivar:not applicable|ecotype:AB|age:5 dpf|dev stage:larvae|collection date:2020 10 03|geo loc name:China: Lanzhou|sex:not determined|tissue:whole body|BioSampleModel:Model organism or animal                 miRNA seq of zebrafish larvae DD 3 DD 3 miRNA seq     miRNA-Seq TRANSCRIPTOMIC RANDOM PCR SINGLE ILLUMINA Illumina HiSeq 3000   SRP512097     DD_3.fq.gz fastq 667459389.0 13087439.0 DD 3.fq.gz 0:51 A:157267065;C:168909886;G:178336724;T:162865168;N:80546 51       157267065 168909886 178336724 162865168 80546 SRX24820190 SRS21534292 SRA1890590 Lanzhou University|College of Life Science Lanzhou University 1 0.19375   0.03528   0.98827   0.55124   51   B   usable mapping rate illumina hiseq_era unknown random_priming unknown bulk unknown unknown   China 2024-06-06 Larval Larval Trunk Surface Structure
32549 32549 SRR29303128 SRX24820189 SRS21534291 SRP512097 PRJNA1120592 Light induce Zebrafish larvae PRJNA1120592 Other Light induce Zebrafish larvae miRNA         L6H 3 miRNA   breed:zebrafish|cultivar:not applicable|ecotype:AB|age:5 dpf|dev stage:larvae|collection date:2020 10 12|geo loc name:China: Lanzhou|sex:not determined|tissue:whole body|BioSampleModel:Model organism or animal                 miRNA seq of zebrafish larvae L6H 3 L6H 3 miRNA seq     miRNA-Seq TRANSCRIPTOMIC RANDOM PCR SINGLE ILLUMINA Illumina HiSeq 3000   SRP512097     L6H_3.fq.gz fastq 551181072.0 10807472.0 L6H 3.fq.gz 0:51 A:126813056;C:141881980;G:147676363;T:134665008;N:144665 51       126813056 141881980 147676363 134665008 144665 SRX24820189 SRS21534291 SRA1890590 Lanzhou University|College of Life Science Lanzhou University 1 0.09301   0.01507   0.99042   0.54323   51   B   usable mapping rate illumina hiseq_era unknown random_priming unknown bulk unknown unknown   China 2024-06-06 Larval Larval Trunk Surface Structure
32550 32550 SRR29303129 SRX24820188 SRS21534290 SRP512097 PRJNA1120592 Light induce Zebrafish larvae PRJNA1120592 Other Light induce Zebrafish larvae miRNA         L6H 2 miRNA   breed:zebrafish|cultivar:not applicable|ecotype:AB|age:5 dpf|dev stage:larvae|collection date:2020 10 11|geo loc name:China: Lanzhou|sex:not determined|tissue:whole body|BioSampleModel:Model organism or animal                 miRNA seq of zebrafish larvae L6H 2 L6H 2 miRNA seq     miRNA-Seq TRANSCRIPTOMIC RANDOM PCR SINGLE ILLUMINA Illumina HiSeq 3000   SRP512097     L6H_2.fq.gz fastq 571762632.0 11211032.0 L6H 2.fq.gz 0:51 A:138521192;C:148068464;G:150615597;T:134408189;N:149190 51       138521192 148068464 150615597 134408189 149190 SRX24820188 SRS21534290 SRA1890590 Lanzhou University|College of Life Science Lanzhou University 1 0.01523   0.00336   0.9933   0.57685   51   B   usable mapping rate illumina hiseq_era unknown random_priming unknown bulk unknown unknown   China 2024-06-06 Larval Larval Trunk Surface Structure
32551 32551 SRR29303130 SRX24820187 SRS21534289 SRP512097 PRJNA1120592 Light induce Zebrafish larvae PRJNA1120592 Other Light induce Zebrafish larvae miRNA         DD 2 miRNA   breed:zebrafish|cultivar:not applicable|ecotype:AB|age:5 dpf|dev stage:larvae|collection date:2020 10 02|geo loc name:China: Lanzhou|sex:not determined|tissue:whole body|BioSampleModel:Model organism or animal                 miRNA seq of zebrafish larvae DD 2 DD 2 miRNA seq     miRNA-Seq TRANSCRIPTOMIC RANDOM PCR SINGLE ILLUMINA Illumina HiSeq 3000   SRP512097     DD_2.fq.gz fastq 706940937.0 13861587.0 DD 2.fq.gz 0:51 A:162899595;C:189209828;G:187422113;T:167222837;N:186564 51       162899595 189209828 187422113 167222837 186564 SRX24820187 SRS21534289 SRA1890590 Lanzhou University|College of Life Science Lanzhou University 1 0.02266   0.00566   0.99334   0.56959   51   B   usable mapping rate illumina hiseq_era unknown random_priming unknown bulk unknown unknown   China 2024-06-06 Larval Larval Trunk Surface Structure
32552 32552 SRR29303131 SRX24820186 SRS21534288 SRP512097 PRJNA1120592 Light induce Zebrafish larvae PRJNA1120592 Other Light induce Zebrafish larvae miRNA         DD 1 miRNA   breed:zebrafish|cultivar:not applicable|ecotype:AB|age:5 dpf|dev stage:larvae|collection date:2020 10 01|geo loc name:China: Lanzhou|sex:not determined|tissue:whole body|BioSampleModel:Model organism or animal                 miRNA seq of zebrafish larvae DD 1 DD 1 miRNA seq     miRNA-Seq TRANSCRIPTOMIC RANDOM PCR SINGLE ILLUMINA Illumina HiSeq 3000   SRP512097     DD_1.fq.gz fastq 540332862.0 10594762.0 DD 1.fq.gz 0:51 A:127336994;C:143901414;G:142437491;T:126515166;N:141797 51       127336994 143901414 142437491 126515166 141797 SRX24820186 SRS21534288 SRA1890590 Lanzhou University|College of Life Science Lanzhou University 1 0.01404   0.00313   0.99484   0.56573   51   B   usable mapping rate illumina hiseq_era unknown random_priming unknown bulk unknown unknown   China 2024-06-06 Larval Larval Trunk Surface Structure
34282 34282 SRR31640757 SRX27004210 SRS23468967 SRP550004 PRJNA1195374 Cortex Dictamni induces retinitis pigmentosa in zebrafish by inhibiting pde6a post transcriptional activity via mmu mir 6240 p3 2 PRJNA1195374 Other Ethnopharmacological relevance: Cortex Dictamni CD is the dried root skin of Dictamnus dasycarpus Turcz widely used in the field of traditional Chinese medicine. mainly for the treatment of skin diseases. Recent adverse reactions to CD limited the clinical application in combination with other traditional Chinese medicines.Aim of the study: To investigate the retinitis pigmentosa RP effects of CD using the zebrafish model and elucidate the underlying molecular mechanism of CD induced RP in zebrafish.Materials and methods: The 3 dpf zebrafish larvae were divided into control and CD group. RNA sequencing followed with qRT PCR validating miRNAs and mRNAs. Dual luciferase reporter assay confirmed mmu mir 6240 p3 2's interaction with pde6a. HT22 cells were transfected treated with CD and evaluated for migration invasion malondialdehyde level superoxide dismutase and acetylcholine activities.Results: The results showed 6228 differentially expressed genes and 66 miRNAs differentially expressed in zebrafish exposed to CD. The personal correlation coefficient results showed that mmu mir 6240 p3 2 had the highest correlation coefficient with pde6a and had a negative regulatory relationship. The results of dual luciferase reporter gene further showed that pde6a gene was the direct target of mmu mir 6240 p3 2. Cell experiment results showed that inhibiting mir 6240 p3 2 can upregulate pde6a expression alleviate HT22 cell injury and reverse the inhibition of cell migration and invasion induced by CD.Conclusions: CD induces RP in zebrafish by inhibiting pde6a post transcriptional activity via mmu mir 6240 p3 2. These findings have important implications for understanding the potential side effects of CD and for developing safer therapeutic strategies involving traditional Chinese medicines.         CD1   strain:not applicable|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|age:not applicable|dev stage:not applicable|collection date:not applicable|geo loc name:not applicable|sex:not applicable|tissue:not applicable|treatment:Experiment group replicate1|BioSampleModel:Model organism or animal                 microRNA seq of Danio rerio: Experiment group BXP 1 BXP 1       miRNA-Seq TRANSCRIPTOMIC RANDOM SINGLE ILLUMINA Illumina NovaSeq 6000   SRP550004     BXP_1.fq.gz fastq 816499035.0 16009785.0 BXP 1.fq.gz 0:51 A:191280900;C:189440771;G:247005615;T:188686493;N:85256 51       191280900 189440771 247005615 188686493 85256 SRX27004210 SRS23468967 SRA2029520 Heilongjiang University of Chinese Medicine|College of Traditional Chinese Medicine Heilongjiang University of Chinese Medicine                       T   under 1.2% mapping rate illumina novaseq_era unknown random_priming unknown bulk unknown unknown   China 2024-12-07 Undetermined Larval Undetermined Undetermined
34283 34283 SRR31640758 SRX27004209 SRS23468966 SRP550004 PRJNA1195374 Cortex Dictamni induces retinitis pigmentosa in zebrafish by inhibiting pde6a post transcriptional activity via mmu mir 6240 p3 2 PRJNA1195374 Other Ethnopharmacological relevance: Cortex Dictamni CD is the dried root skin of Dictamnus dasycarpus Turcz widely used in the field of traditional Chinese medicine. mainly for the treatment of skin diseases. Recent adverse reactions to CD limited the clinical application in combination with other traditional Chinese medicines.Aim of the study: To investigate the retinitis pigmentosa RP effects of CD using the zebrafish model and elucidate the underlying molecular mechanism of CD induced RP in zebrafish.Materials and methods: The 3 dpf zebrafish larvae were divided into control and CD group. RNA sequencing followed with qRT PCR validating miRNAs and mRNAs. Dual luciferase reporter assay confirmed mmu mir 6240 p3 2's interaction with pde6a. HT22 cells were transfected treated with CD and evaluated for migration invasion malondialdehyde level superoxide dismutase and acetylcholine activities.Results: The results showed 6228 differentially expressed genes and 66 miRNAs differentially expressed in zebrafish exposed to CD. The personal correlation coefficient results showed that mmu mir 6240 p3 2 had the highest correlation coefficient with pde6a and had a negative regulatory relationship. The results of dual luciferase reporter gene further showed that pde6a gene was the direct target of mmu mir 6240 p3 2. Cell experiment results showed that inhibiting mir 6240 p3 2 can upregulate pde6a expression alleviate HT22 cell injury and reverse the inhibition of cell migration and invasion induced by CD.Conclusions: CD induces RP in zebrafish by inhibiting pde6a post transcriptional activity via mmu mir 6240 p3 2. These findings have important implications for understanding the potential side effects of CD and for developing safer therapeutic strategies involving traditional Chinese medicines.         Control3   strain:not applicable|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|age:not applicable|dev stage:not applicable|collection date:not applicable|geo loc name:not applicable|sex:not applicable|tissue:not applicable|treatment:Control group replicate3|BioSampleModel:Model organism or animal                 microRNA seq of Danio rerio: Control group Control 3 Control 3       miRNA-Seq TRANSCRIPTOMIC RANDOM SINGLE ILLUMINA Illumina NovaSeq 6000   SRP550004     Control_3.fq.gz fastq 662560023.0 12991373.0 Control 3.fq.gz 0:51 A:151053320;C:152811933;G:203340354;T:155283230;N:71186 51       151053320 152811933 203340354 155283230 71186 SRX27004209 SRS23468966 SRA2029520 Heilongjiang University of Chinese Medicine|College of Traditional Chinese Medicine Heilongjiang University of Chinese Medicine                       T   under 1.2% mapping rate illumina novaseq_era unknown random_priming unknown bulk unknown unknown   China 2024-12-07 Undetermined Larval Undetermined Undetermined
34284 34284 SRR31640759 SRX27004208 SRS23468963 SRP550004 PRJNA1195374 Cortex Dictamni induces retinitis pigmentosa in zebrafish by inhibiting pde6a post transcriptional activity via mmu mir 6240 p3 2 PRJNA1195374 Other Ethnopharmacological relevance: Cortex Dictamni CD is the dried root skin of Dictamnus dasycarpus Turcz widely used in the field of traditional Chinese medicine. mainly for the treatment of skin diseases. Recent adverse reactions to CD limited the clinical application in combination with other traditional Chinese medicines.Aim of the study: To investigate the retinitis pigmentosa RP effects of CD using the zebrafish model and elucidate the underlying molecular mechanism of CD induced RP in zebrafish.Materials and methods: The 3 dpf zebrafish larvae were divided into control and CD group. RNA sequencing followed with qRT PCR validating miRNAs and mRNAs. Dual luciferase reporter assay confirmed mmu mir 6240 p3 2's interaction with pde6a. HT22 cells were transfected treated with CD and evaluated for migration invasion malondialdehyde level superoxide dismutase and acetylcholine activities.Results: The results showed 6228 differentially expressed genes and 66 miRNAs differentially expressed in zebrafish exposed to CD. The personal correlation coefficient results showed that mmu mir 6240 p3 2 had the highest correlation coefficient with pde6a and had a negative regulatory relationship. The results of dual luciferase reporter gene further showed that pde6a gene was the direct target of mmu mir 6240 p3 2. Cell experiment results showed that inhibiting mir 6240 p3 2 can upregulate pde6a expression alleviate HT22 cell injury and reverse the inhibition of cell migration and invasion induced by CD.Conclusions: CD induces RP in zebrafish by inhibiting pde6a post transcriptional activity via mmu mir 6240 p3 2. These findings have important implications for understanding the potential side effects of CD and for developing safer therapeutic strategies involving traditional Chinese medicines.         Control2   strain:not applicable|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|age:not applicable|dev stage:not applicable|collection date:not applicable|geo loc name:not applicable|sex:not applicable|tissue:not applicable|treatment:Control group replicate2|BioSampleModel:Model organism or animal                 microRNA seq of Danio rerio: Control group Control 2 Control 2       miRNA-Seq TRANSCRIPTOMIC RANDOM SINGLE ILLUMINA Illumina NovaSeq 6000   SRP550004     Control_2.fq.gz fastq 648856119.0 12722669.0 Control 2.fq.gz 0:51 A:149869576;C:151844056;G:195830503;T:151241215;N:70769 51       149869576 151844056 195830503 151241215 70769 SRX27004208 SRS23468963 SRA2029520 Heilongjiang University of Chinese Medicine|College of Traditional Chinese Medicine Heilongjiang University of Chinese Medicine                       T   under 1.2% mapping rate illumina novaseq_era unknown random_priming unknown bulk unknown unknown   China 2024-12-07 Undetermined Larval Undetermined Undetermined
34285 34285 SRR31640760 SRX27004207 SRS23468962 SRP550004 PRJNA1195374 Cortex Dictamni induces retinitis pigmentosa in zebrafish by inhibiting pde6a post transcriptional activity via mmu mir 6240 p3 2 PRJNA1195374 Other Ethnopharmacological relevance: Cortex Dictamni CD is the dried root skin of Dictamnus dasycarpus Turcz widely used in the field of traditional Chinese medicine. mainly for the treatment of skin diseases. Recent adverse reactions to CD limited the clinical application in combination with other traditional Chinese medicines.Aim of the study: To investigate the retinitis pigmentosa RP effects of CD using the zebrafish model and elucidate the underlying molecular mechanism of CD induced RP in zebrafish.Materials and methods: The 3 dpf zebrafish larvae were divided into control and CD group. RNA sequencing followed with qRT PCR validating miRNAs and mRNAs. Dual luciferase reporter assay confirmed mmu mir 6240 p3 2's interaction with pde6a. HT22 cells were transfected treated with CD and evaluated for migration invasion malondialdehyde level superoxide dismutase and acetylcholine activities.Results: The results showed 6228 differentially expressed genes and 66 miRNAs differentially expressed in zebrafish exposed to CD. The personal correlation coefficient results showed that mmu mir 6240 p3 2 had the highest correlation coefficient with pde6a and had a negative regulatory relationship. The results of dual luciferase reporter gene further showed that pde6a gene was the direct target of mmu mir 6240 p3 2. Cell experiment results showed that inhibiting mir 6240 p3 2 can upregulate pde6a expression alleviate HT22 cell injury and reverse the inhibition of cell migration and invasion induced by CD.Conclusions: CD induces RP in zebrafish by inhibiting pde6a post transcriptional activity via mmu mir 6240 p3 2. These findings have important implications for understanding the potential side effects of CD and for developing safer therapeutic strategies involving traditional Chinese medicines.         Control1   strain:not applicable|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|age:not applicable|dev stage:not applicable|collection date:not applicable|geo loc name:not applicable|sex:not applicable|tissue:not applicable|treatment:Control group replicate1|BioSampleModel:Model organism or animal                 microRNA seq of Danio rerio: Control group Control 1 Control 1       miRNA-Seq TRANSCRIPTOMIC RANDOM SINGLE ILLUMINA Illumina NovaSeq 6000   SRP550004     Control_1.fq.gz fastq 835887501.0 16389951.0 Control 1.fq.gz 0:51 A:193253330;C:196900031;G:252690178;T:192797500;N:246462 51       193253330 196900031 252690178 192797500 246462 SRX27004207 SRS23468962 SRA2029520 Heilongjiang University of Chinese Medicine|College of Traditional Chinese Medicine Heilongjiang University of Chinese Medicine                       T   under 1.2% mapping rate illumina novaseq_era unknown random_priming unknown bulk unknown unknown   China 2024-12-07 Undetermined Larval Undetermined Undetermined
34290 34290 SRR31640765 SRX27004202 SRS23468965 SRP550004 PRJNA1195374 Cortex Dictamni induces retinitis pigmentosa in zebrafish by inhibiting pde6a post transcriptional activity via mmu mir 6240 p3 2 PRJNA1195374 Other Ethnopharmacological relevance: Cortex Dictamni CD is the dried root skin of Dictamnus dasycarpus Turcz widely used in the field of traditional Chinese medicine. mainly for the treatment of skin diseases. Recent adverse reactions to CD limited the clinical application in combination with other traditional Chinese medicines.Aim of the study: To investigate the retinitis pigmentosa RP effects of CD using the zebrafish model and elucidate the underlying molecular mechanism of CD induced RP in zebrafish.Materials and methods: The 3 dpf zebrafish larvae were divided into control and CD group. RNA sequencing followed with qRT PCR validating miRNAs and mRNAs. Dual luciferase reporter assay confirmed mmu mir 6240 p3 2's interaction with pde6a. HT22 cells were transfected treated with CD and evaluated for migration invasion malondialdehyde level superoxide dismutase and acetylcholine activities.Results: The results showed 6228 differentially expressed genes and 66 miRNAs differentially expressed in zebrafish exposed to CD. The personal correlation coefficient results showed that mmu mir 6240 p3 2 had the highest correlation coefficient with pde6a and had a negative regulatory relationship. The results of dual luciferase reporter gene further showed that pde6a gene was the direct target of mmu mir 6240 p3 2. Cell experiment results showed that inhibiting mir 6240 p3 2 can upregulate pde6a expression alleviate HT22 cell injury and reverse the inhibition of cell migration and invasion induced by CD.Conclusions: CD induces RP in zebrafish by inhibiting pde6a post transcriptional activity via mmu mir 6240 p3 2. These findings have important implications for understanding the potential side effects of CD and for developing safer therapeutic strategies involving traditional Chinese medicines.         CD3   strain:not applicable|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|age:not applicable|dev stage:not applicable|collection date:not applicable|geo loc name:not applicable|sex:not applicable|tissue:not applicable|treatment:Experiment group replicate3|BioSampleModel:Model organism or animal                 microRNA seq of Danio rerio: Experiment group BXP 3 BXP 3       miRNA-Seq TRANSCRIPTOMIC RANDOM SINGLE ILLUMINA Illumina NovaSeq 6000   SRP550004     BXP_3.fq.gz fastq 656139327.0 12865477.0 BXP 3.fq.gz 0:51 A:152349522;C:156353407;G:196379918;T:150985088;N:71392 51       152349522 156353407 196379918 150985088 71392 SRX27004202 SRS23468965 SRA2029520 Heilongjiang University of Chinese Medicine|College of Traditional Chinese Medicine Heilongjiang University of Chinese Medicine                       T   under 1.2% mapping rate illumina novaseq_era unknown random_priming unknown bulk unknown unknown   China 2024-12-07 Undetermined Larval Undetermined Undetermined
34291 34291 SRR31640766 SRX27004201 SRS23468964 SRP550004 PRJNA1195374 Cortex Dictamni induces retinitis pigmentosa in zebrafish by inhibiting pde6a post transcriptional activity via mmu mir 6240 p3 2 PRJNA1195374 Other Ethnopharmacological relevance: Cortex Dictamni CD is the dried root skin of Dictamnus dasycarpus Turcz widely used in the field of traditional Chinese medicine. mainly for the treatment of skin diseases. Recent adverse reactions to CD limited the clinical application in combination with other traditional Chinese medicines.Aim of the study: To investigate the retinitis pigmentosa RP effects of CD using the zebrafish model and elucidate the underlying molecular mechanism of CD induced RP in zebrafish.Materials and methods: The 3 dpf zebrafish larvae were divided into control and CD group. RNA sequencing followed with qRT PCR validating miRNAs and mRNAs. Dual luciferase reporter assay confirmed mmu mir 6240 p3 2's interaction with pde6a. HT22 cells were transfected treated with CD and evaluated for migration invasion malondialdehyde level superoxide dismutase and acetylcholine activities.Results: The results showed 6228 differentially expressed genes and 66 miRNAs differentially expressed in zebrafish exposed to CD. The personal correlation coefficient results showed that mmu mir 6240 p3 2 had the highest correlation coefficient with pde6a and had a negative regulatory relationship. The results of dual luciferase reporter gene further showed that pde6a gene was the direct target of mmu mir 6240 p3 2. Cell experiment results showed that inhibiting mir 6240 p3 2 can upregulate pde6a expression alleviate HT22 cell injury and reverse the inhibition of cell migration and invasion induced by CD.Conclusions: CD induces RP in zebrafish by inhibiting pde6a post transcriptional activity via mmu mir 6240 p3 2. These findings have important implications for understanding the potential side effects of CD and for developing safer therapeutic strategies involving traditional Chinese medicines.         CD2   strain:not applicable|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|age:not applicable|dev stage:not applicable|collection date:not applicable|geo loc name:not applicable|sex:not applicable|tissue:not applicable|treatment:Experiment group replicate2|BioSampleModel:Model organism or animal                 microRNA seq of Danio rerio: Experiment group BXP 2 BXP 2       miRNA-Seq TRANSCRIPTOMIC RANDOM SINGLE ILLUMINA Illumina NovaSeq 6000   SRP550004     BXP_2.fq.gz fastq 644434827.0 12635977.0 BXP 2.fq.gz 0:51 A:151820167;C:153674905;G:190467723;T:148402934;N:69098 51       151820167 153674905 190467723 148402934 69098 SRX27004201 SRS23468964 SRA2029520 Heilongjiang University of Chinese Medicine|College of Traditional Chinese Medicine Heilongjiang University of Chinese Medicine                       T   under 1.2% mapping rate illumina novaseq_era unknown random_priming unknown bulk unknown unknown   China 2024-12-07 Undetermined Larval Undetermined Undetermined
36560 36560 SRR1048073 SRX363298 SRS369337 SRP016132 PRJNA177642 Danio rerio Transcriptome or Gene expression PRJNA177642 Other Pineal glands of adult zebrafish were sampled every 4 hours for 48 hours.     Adult 0.5 1.5 years old transgenic zebrafish Tgaanat2:EGFPY8 which express enhanced green fluorescent protein EGFP in the pineal gland under the control of the aanat2 regulatory regions were used. Fish were raised under 12 hr light:12 hr dark LD cycles in a temperature controlled room and transferred to constant darkness DD for tissue collection. Fish were anesthetized in 1.5 mM Tricane Sigma sacrificed by decapitation and pineal glands were removed under a fluorescent dissecting microscope. Starting from circadian time CT 14 pineal glands were collected at 4 hr intervals for 48 hours 12 time points identified as CT 14 18 22 2 6 10 14b 18b 22b 2b 6b and 10b. Pools of 20 pineal glands were prepared at each time point and total RNA was extracted using the RNeasy Lipid Tissue Mini Kit QIAGEN according to the manufacturer's instructions. Illumina TruSeq protocol was used to prepare libraries from RNA samples. Overall 12 libraries 12 time points were run on 2 lanes of Illumina HiSeq2000 machine using the multiplexing strategy of the TruSeq protocol Institute of Applied Genomics. On average 30 million paired end reads were obtained for each library. The reads were 2 x 100 base pairs for 8 time points CTs 22 2 6 10 18b 2b 6b 10b and 2 x 50 base pairs for the remaining time points CTs 14 18 14b and 22b. Zebrafish pineal gland Pineal gland                     Zebrafish Brain miRNA Brain miRNA 1 Adult 0.5 1.5 years old Tgaanat2:EGFP zebrafish Gothilf et al. 2002 were raised in a temperature controlled recirculation water system under 12 hr light: 12 hr dark LD cycles and transferred to constant darkness DD at the end of the day prior to the experiment. Fish were exposed to a 1 hr light pulse light intensity of 12 W/m2 prior to sampling light treatment or kept under constant darkness for control dark treatment. The fish were anesthetized in 1.5mM Tricane Sigma and sacrificed by decapitation. Pineal glands and brains were removed under a fluorescent dissecting microscope; the use of transgenic fish expressing enhanced green fluorescent protein EGFP in the pineal gland enabled its selective removal. The tissues were collected from light and dark treated fish at 6 time points with 4 hr intervals throughout one daily cycle corresponding to CT2 6 10 14 18 and 22. For miR seq 18 pineal glands were pooled from the 6 sampling times for both light and dark treatments and one brain pool was composed of 4 brains that were collected from light and dark treated fish at CT2 and CT14. Brain samples were collected subsequent to the removal of pineal glands and therefore do not contain pineal tissues. Total RNA for miRNA analysis was isolated using miRNeasy Mini Kit Qiagen. miRNA capturing and library construction for the pineal light pineal dark and brain samples were conducted using Illumina's TruSeq Small RNA Sample Prep Kit according to the manufacturer’s protocol. Briefly three prime adapter was ligated to total RNA using truncated T4 RNA Ligase 2 without xxx then five prime adapter was added using T4 RNA Ligase with ATP. postwards reverse transcription followed by PCR was used to create cDNA constructs based on the small RNA ligated with three prime and five prime adapters. The introduction of index sequence bar codes was done at the PCR step. Finally gel electrophoresis was used to purify the amplified cDNA construct in preparation for subsequent cluster generation. post library quality check by …     miRNA-Seq TRANSCRIPTOMIC size fractionation SINGLE ILLUMINA Illumina HiSeq 2000 <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>50</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> SRP016132     ZB_trimmed.fastq fastq 323899236.0 14781569.0 Brain miRNA run 0:21.91 1:0 A:77705253;C:63882329;G:85902986;T:96383552;N:25116 21 0     77705253 63882329 85902986 96383552 25116 SRX363298 SRS369337 SRA054264 Tel Aviv University Tel Aviv University 1 0.88754   0.06569   0.96747   0.53358   23   B   usable mapping rate illumina hiseq_era unknown size_fractionation trueseq bulk unknown unknown   Israel 2015-07-22 Adult Adult Multi-tissue Multi-system
36561 36561 SRR1048072 SRX363297 SRS369337 SRP016132 PRJNA177642 Danio rerio Transcriptome or Gene expression PRJNA177642 Other Pineal glands of adult zebrafish were sampled every 4 hours for 48 hours.     Adult 0.5 1.5 years old transgenic zebrafish Tgaanat2:EGFPY8 which express enhanced green fluorescent protein EGFP in the pineal gland under the control of the aanat2 regulatory regions were used. Fish were raised under 12 hr light:12 hr dark LD cycles in a temperature controlled room and transferred to constant darkness DD for tissue collection. Fish were anesthetized in 1.5 mM Tricane Sigma sacrificed by decapitation and pineal glands were removed under a fluorescent dissecting microscope. Starting from circadian time CT 14 pineal glands were collected at 4 hr intervals for 48 hours 12 time points identified as CT 14 18 22 2 6 10 14b 18b 22b 2b 6b and 10b. Pools of 20 pineal glands were prepared at each time point and total RNA was extracted using the RNeasy Lipid Tissue Mini Kit QIAGEN according to the manufacturer's instructions. Illumina TruSeq protocol was used to prepare libraries from RNA samples. Overall 12 libraries 12 time points were run on 2 lanes of Illumina HiSeq2000 machine using the multiplexing strategy of the TruSeq protocol Institute of Applied Genomics. On average 30 million paired end reads were obtained for each library. The reads were 2 x 100 base pairs for 8 time points CTs 22 2 6 10 18b 2b 6b 10b and 2 x 50 base pairs for the remaining time points CTs 14 18 14b and 22b. Zebrafish pineal gland Pineal gland                     Zebrafish pineal gland dark miRNA Pineal gland dark miRNA 1 Adult 0.5 1.5 years old Tgaanat2:EGFP zebrafish Gothilf et al. 2002 were raised in a temperature controlled recirculation water system under 12 hr light: 12 hr dark LD cycles and transferred to constant darkness DD at the end of the day prior to the experiment. Fish were exposed to a 1 hr light pulse light intensity of 12 W/m2 prior to sampling light treatment or kept under constant darkness for control dark treatment. The fish were anesthetized in 1.5mM Tricane Sigma and sacrificed by decapitation. Pineal glands and brains were removed under a fluorescent dissecting microscope; the use of transgenic fish expressing enhanced green fluorescent protein EGFP in the pineal gland enabled its selective removal. The tissues were collected from light and dark treated fish at 6 time points with 4 hr intervals throughout one daily cycle corresponding to CT2 6 10 14 18 and 22. For miR seq 18 pineal glands were pooled from the 6 sampling times for both light and dark treatments and one brain pool was composed of 4 brains that were collected from light and dark treated fish at CT2 and CT14. Brain samples were collected subsequent to the removal of pineal glands and therefore do not contain pineal tissues. Total RNA for miRNA analysis was isolated using miRNeasy Mini Kit Qiagen. miRNA capturing and library construction for the pineal light pineal dark and brain samples were conducted using Illumina's TruSeq Small RNA Sample Prep Kit according to the manufacturer’s protocol. Briefly three prime adapter was ligated to total RNA using truncated T4 RNA Ligase 2 without xxx then five prime adapter was added using T4 RNA Ligase with ATP. postwards reverse transcription followed by PCR was used to create cDNA constructs based on the small RNA ligated with three prime and five prime adapters. The introduction of index sequence bar codes was done at the PCR step. Finally gel electrophoresis was used to purify the amplified cDNA construct in preparation for subsequent cluster generation. post library quality check by …     miRNA-Seq TRANSCRIPTOMIC size fractionation SINGLE ILLUMINA Illumina HiSeq 2000 <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>50</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> SRP016132     D_trimmed.fastq fastq 231371620.0 10385264.0 Pineal gland dark miRNA run 0:22.28 1:0 A:61228408;C:44264639;G:58700027;T:67159878;N:18668 22 0     61228408 44264639 58700027 67159878 18668 SRX363297 SRS369337 SRA054264 Tel Aviv University Tel Aviv University 1 0.84648   0.04467   0.95645   0.79978   22   B   usable mapping rate illumina hiseq_era unknown size_fractionation trueseq bulk unknown unknown   Israel 2015-07-22 Adult Adult Multi-tissue Multi-system
36562 36562 SRR1048071 SRX363296 SRS369337 SRP016132 PRJNA177642 Danio rerio Transcriptome or Gene expression PRJNA177642 Other Pineal glands of adult zebrafish were sampled every 4 hours for 48 hours.     Adult 0.5 1.5 years old transgenic zebrafish Tgaanat2:EGFPY8 which express enhanced green fluorescent protein EGFP in the pineal gland under the control of the aanat2 regulatory regions were used. Fish were raised under 12 hr light:12 hr dark LD cycles in a temperature controlled room and transferred to constant darkness DD for tissue collection. Fish were anesthetized in 1.5 mM Tricane Sigma sacrificed by decapitation and pineal glands were removed under a fluorescent dissecting microscope. Starting from circadian time CT 14 pineal glands were collected at 4 hr intervals for 48 hours 12 time points identified as CT 14 18 22 2 6 10 14b 18b 22b 2b 6b and 10b. Pools of 20 pineal glands were prepared at each time point and total RNA was extracted using the RNeasy Lipid Tissue Mini Kit QIAGEN according to the manufacturer's instructions. Illumina TruSeq protocol was used to prepare libraries from RNA samples. Overall 12 libraries 12 time points were run on 2 lanes of Illumina HiSeq2000 machine using the multiplexing strategy of the TruSeq protocol Institute of Applied Genomics. On average 30 million paired end reads were obtained for each library. The reads were 2 x 100 base pairs for 8 time points CTs 22 2 6 10 18b 2b 6b 10b and 2 x 50 base pairs for the remaining time points CTs 14 18 14b and 22b. Zebrafish pineal gland Pineal gland                     Zebrafish pineal gland light miRNA Pineal gland light miRNA 1 Adult 0.5 1.5 years old Tgaanat2:EGFP zebrafish Gothilf et al. 2002 were raised in a temperature controlled recirculation water system under 12 hr light: 12 hr dark LD cycles and transferred to constant darkness DD at the end of the day prior to the experiment. Fish were exposed to a 1 hr light pulse light intensity of 12 W/m2 prior to sampling light treatment or kept under constant darkness for control dark treatment. The fish were anesthetized in 1.5mM Tricane Sigma and sacrificed by decapitation. Pineal glands and brains were removed under a fluorescent dissecting microscope; the use of transgenic fish expressing enhanced green fluorescent protein EGFP in the pineal gland enabled its selective removal. The tissues were collected from light and dark treated fish at 6 time points with 4 hr intervals throughout one daily cycle corresponding to CT2 6 10 14 18 and 22. For miR seq 18 pineal glands were pooled from the 6 sampling times for both light and dark treatments and one brain pool was composed of 4 brains that were collected from light and dark treated fish at CT2 and CT14. Brain samples were collected subsequent to the removal of pineal glands and therefore do not contain pineal tissues. Total RNA for miRNA analysis was isolated using miRNeasy Mini Kit Qiagen. miRNA capturing and library construction for the pineal light pineal dark and brain samples were conducted using Illumina's TruSeq Small RNA Sample Prep Kit according to the manufacturer’s protocol. Briefly three prime adapter was ligated to total RNA using truncated T4 RNA Ligase 2 without xxx then five prime adapter was added using T4 RNA Ligase with ATP. postwards reverse transcription followed by PCR was used to create cDNA constructs based on the small RNA ligated with three prime and five prime adapters. The introduction of index sequence bar codes was done at the PCR step. Finally gel electrophoresis was used to purify the amplified cDNA construct in preparation for subsequent cluster generation. post library quality check by …     miRNA-Seq TRANSCRIPTOMIC size fractionation SINGLE ILLUMINA Illumina HiSeq 2000 <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>50</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> SRP016132     L_trimmed.fastq fastq 132417471.0 5933638.0 Pineal gland light miRNA run 0:22.32 1:0 A:36017069;C:26569684;G:32546616;T:37273526;N:10576 22 0     36017069 26569684 32546616 37273526 10576 SRX363296 SRS369337 SRA054264 Tel Aviv University Tel Aviv University 1 0.86427   0.03426   0.95631   0.86509   21   B   usable mapping rate illumina hiseq_era unknown size_fractionation trueseq bulk unknown unknown   Israel 2015-07-22 Adult Adult Multi-tissue Multi-system
37921 37921 SRR1554495 SRX685396 SRS686645 SRP039502 PRJNA240316 Danio rerio strain:AB Transcriptome or Gene expression PRJNA240316 Transcriptome Analysis Quantifying expression levels of smallRNAs between tissues in Danio Rerio strain AB.   pubmed:24835514     ZF4Ovary1   strain:AB|age:Reproductive Adult|biomaterial provider:Postlethwait Lab Institute of Neuroscience 1254 University of Oregon 222 Huestis Hall Eugene OR 97410|sex:female|tissue:Ovary|birth location:University of Oregon Fish Facility|collected by:Thomas Desvignes|collection date:2013 10 07|BioSampleModel:Model organism or animal                 Zebrafish Ovary2 ZF4Ovary2 1 Tissue specific sequencing libraries were prepped and barcoded using the BiooScientific NEXTflex smallRNA Sequencing Kit which uses a 3’ adenylated adapter that ligates onto miRs and other small RNAs with a 3’ hydroxyl group.     miRNA-Seq TRANSCRIPTOMIC unspecified SINGLE ILLUMINA Illumina HiSeq 2500 <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>101</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> SRP039502     ZF4_Ovary_3.fq.gz fastq 1225610154.0 12134754.0 Ovary2 Run3 0:101 A:347141358;C:303255805;G:280185540;T:294456010;N:571441 101       347141358 303255805 280185540 294456010 571441 SRX685396 SRS686645 SRA142461 University of Oregon|JHP-Lab University of Oregon 1 2e-05   1e-05   1.0       101   T   under 1.2% mapping rate illumina hiseq_era unknown small_rna unknown bulk unknown unknown   United States 2014-08-22 Adult Adult Gonad Reproductive System
37922 37922 SRR1554492 SRX685395 SRS686644 SRP039502 PRJNA240316 Danio rerio strain:AB Transcriptome or Gene expression PRJNA240316 Transcriptome Analysis Quantifying expression levels of smallRNAs between tissues in Danio Rerio strain AB.   pubmed:24835514     ZF2Testis1   strain:AB|age:Reproductive Adult|biomaterial provider:Postlethwait Lab Institute of Neuroscience 1254 University of Oregon 222 Huestis Hall Eugene OR 97409|sex:male|tissue:Testis|birth location:University of Oregon Fish Facility|collected by:Thomas Desvignes|collection date:2013 10 07|BioSampleModel:Model organism or animal                 Zebrafish Testis2 ZF2Testis2 1 Tissue specific sequencing libraries were prepped and barcoded using the BiooScientific NEXTflex smallRNA Sequencing Kit which uses a 3’ adenylated adapter that ligates onto miRs and other small RNAs with a 3’ hydroxyl group.     miRNA-Seq TRANSCRIPTOMIC unspecified SINGLE ILLUMINA Illumina HiSeq 2500 <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>101</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> SRP039502     ZF2_Testis_3.fq.gz fastq 1192024422.0 11802222.0 Testis2 Run3 0:101 A:373380865;C:276125943;G:251033102;T:290931637;N:552875 101       373380865 276125943 251033102 290931637 552875 SRX685395 SRS686644 SRA142461 University of Oregon|JHP-Lab University of Oregon 1 3e-05   1e-05   0.99997   0.0   101   T   under 1.2% mapping rate illumina hiseq_era unknown small_rna unknown bulk unknown unknown   United States 2014-08-22 Adult Adult Gonad Reproductive System
37923 37923 SRR1554489 SRX685394 SRS686642 SRP039502 PRJNA240316 Danio rerio strain:AB Transcriptome or Gene expression PRJNA240316 Transcriptome Analysis Quantifying expression levels of smallRNAs between tissues in Danio Rerio strain AB.   pubmed:24835514     ZF2Heart1   strain:AB|age:Reproductive Adult|biomaterial provider:Postlethwait Lab Institute of Neuroscience 1254 University of Oregon 222 Huestis Hall Eugene OR 97408|sex:male|tissue:Heart|birth location:University of Oregon Fish Facility|collected by:Thomas Desvignes|collection date:2013 10 07|BioSampleModel:Model organism or animal                 Zebrafish Heart2 ZF2Heart2 1 Tissue specific sequencing libraries were prepped and barcoded using the BiooScientific NEXTflex smallRNA Sequencing Kit which uses a 3’ adenylated adapter that ligates onto miRs and other small RNAs with a 3’ hydroxyl group.     miRNA-Seq TRANSCRIPTOMIC unspecified SINGLE ILLUMINA Illumina HiSeq 2500 <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>101</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> SRP039502     ZF2_Heart_3.fq.gz fastq 1302765670.0 12898670.0 Heart2 Run3 0:101 A:397689061;C:312745449;G:268626802;T:323141419;N:562939 101       397689061 312745449 268626802 323141419 562939 SRX685394 SRS686642 SRA142461 University of Oregon|JHP-Lab University of Oregon 1 3e-05   2e-05   0.99997   1.0   101   T   under 1.2% mapping rate illumina hiseq_era unknown small_rna unknown bulk unknown unknown   United States 2014-08-22 Adult Adult Heart Cardiovascular System
37924 37924 SRR1554486 SRX685392 SRS686641 SRP039502 PRJNA240316 Danio rerio strain:AB Transcriptome or Gene expression PRJNA240316 Transcriptome Analysis Quantifying expression levels of smallRNAs between tissues in Danio Rerio strain AB.   pubmed:24835514     ZF2Brain1   strain:AB|age:Reproductive Adult|biomaterial provider:Postlethwait Lab Institute of Neuroscience 1254 University of Oregon 222 Huestis Hall Eugene OR 97407|sex:male|tissue:Brain|birth location:University of Oregon Fish Facility|collected by:Thomas Desvignes|collection date:2013 10 07|BioSampleModel:Model organism or animal                 Zebrafish Brain2 ZF2Brain2 1 Tissue specific sequencing libraries were prepped and barcoded using the BiooScientific NEXTflex smallRNA Sequencing Kit which uses a 3’ adenylated adapter that ligates onto miRs and other small RNAs with a 3’ hydroxyl group.     miRNA-Seq TRANSCRIPTOMIC unspecified SINGLE ILLUMINA Illumina HiSeq 2500 <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>101</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> SRP039502     ZF2_Brain_3.fq.gz fastq 1211892536.0 11998936.0 Brain2 Run3 0:101 A:374228993;C:286134640;G:253944548;T:297038575;N:545780 101       374228993 286134640 253944548 297038575 545780 SRX685392 SRS686641 SRA142461 University of Oregon|JHP-Lab University of Oregon 1 0.0   0.0   1.0       101   T   under 1.2% mapping rate illumina hiseq_era unknown small_rna unknown bulk unknown unknown   United States 2014-08-22 Adult Adult Brain Nervous System
37925 37925 SRR1554483 SRX685391 SRS686559 SRP039502 PRJNA240316 Danio rerio strain:AB Transcriptome or Gene expression PRJNA240316 Transcriptome Analysis Quantifying expression levels of smallRNAs between tissues in Danio Rerio strain AB.   pubmed:24835514     ZF3Ovary1   strain:AB|age:Reproductive Adult|biomaterial provider:Postlethwait Lab Institute of Neuroscience 1254 University of Oregon 222 Huestis Hall Eugene OR 97406|sex:female|tissue:Ovary|birth location:University of Oregon Fish Facility|collected by:Thomas Desvignes|collection date:2013 10 07|BioSampleModel:Model organism or animal                 Zebrafish Ovary1 ZF3Ovary2 1 Tissue specific sequencing libraries were prepped and barcoded using the BiooScientific NEXTflex smallRNA Sequencing Kit which uses a 3’ adenylated adapter that ligates onto miRs and other small RNAs with a 3’ hydroxyl group.     miRNA-Seq TRANSCRIPTOMIC unspecified SINGLE ILLUMINA Illumina HiSeq 2500 <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>101</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> SRP039502     ZF3_Ovary_3.fq.gz fastq 347516154.0 3440754.0 Ovary1 Run3 0:101 A:107805981;C:80183725;G:73617313;T:85763181;N:145954 101       107805981 80183725 73617313 85763181 145954 SRX685391 SRS686559 SRA142461 University of Oregon|JHP-Lab University of Oregon 1 5e-05   4e-05   1.0       101   T   under 1.2% mapping rate illumina hiseq_era unknown small_rna unknown bulk unknown unknown   United States 2014-08-22 Adult Adult Gonad Reproductive System
37926 37926 SRR1554480 SRX685390 SRS686542 SRP039502 PRJNA240316 Danio rerio strain:AB Transcriptome or Gene expression PRJNA240316 Transcriptome Analysis Quantifying expression levels of smallRNAs between tissues in Danio Rerio strain AB.   pubmed:24835514     ZF1Testis1   strain:AB|age:Reproductive Adult|biomaterial provider:Postlethwait Lab Institute of Neuroscience 1254 University of Oregon 222 Huestis Hall Eugene OR 97405|sex:male|tissue:Testis|birth location:University of Oregon Fish Facility|collected by:Thomas Desvignes|collection date:2013 10 07|BioSampleModel:Model organism or animal                 Zebrafish Testis1 ZF1Testis2 1 Tissue specific sequencing libraries were prepped and barcoded using the BiooScientific NEXTflex smallRNA Sequencing Kit which uses a 3’ adenylated adapter that ligates onto miRs and other small RNAs with a 3’ hydroxyl group.     miRNA-Seq TRANSCRIPTOMIC unspecified SINGLE ILLUMINA Illumina HiSeq 2500 <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>101</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> SRP039502     ZF1_Testis_3.fq.gz fastq 1184228636.0 11725036.0 Testis1 Run3 0:101 A:340636900;C:278505979;G:259975802;T:304564724;N:545231 101       340636900 278505979 259975802 304564724 545231 SRX685390 SRS686542 SRA142461 University of Oregon|JHP-Lab University of Oregon 1 1e-05   0.0   1.0       101   T   under 1.2% mapping rate illumina hiseq_era unknown small_rna unknown bulk unknown unknown   United States 2014-08-22 Adult Adult Gonad Reproductive System
37927 37927 SRR1554477 SRX685389 SRS566618 SRP039502 PRJNA240316 Danio rerio strain:AB Transcriptome or Gene expression PRJNA240316 Transcriptome Analysis Quantifying expression levels of smallRNAs between tissues in Danio Rerio strain AB.   pubmed:24835514     ZF1Heart1   strain:AB|age:Reproductive Adult|biomaterial provider:Postlethwait Lab Institute of Neuroscience 1254 University of Oregon 222 Huestis Hall Eugene OR 97404|sex:male|tissue:Heart|birth location:University of Oregon Fish Facility|collected by:Thomas Desvignes|collection date:2013 10 07|BioSampleModel:Model organism or animal                 Zebrafish individual 1 heart ZF1Heart2 1 Tissue specific sequencing libraries were prepped and barcoded using the BiooScientific NEXTflex smallRNA Sequencing Kit which uses a 3’ adenylated adapter that ligates onto miRs and other small RNAs with a 3’ hydroxyl group.     miRNA-Seq TRANSCRIPTOMIC unspecified SINGLE ILLUMINA Illumina HiSeq 2500 <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>101</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> SRP039502     ZF1_Heart_3.fq.gz fastq 1561371524.0 15459124.0 Heart1 Run3 0:101 A:454757966;C:391143691;G:334715236;T:380114024;N:640607 101       454757966 391143691 334715236 380114024 640607 SRX685389 SRS566618 SRA142461 University of Oregon|JHP-Lab University of Oregon 1 3e-05   2e-05   0.99997   1.0   101   T   under 1.2% mapping rate illumina hiseq_era unknown small_rna unknown bulk unknown unknown   United States 2014-08-22 Adult Adult Heart Cardiovascular System
37928 37928 SRR1554474 SRX685388 SRS566490 SRP039502 PRJNA240316 Danio rerio strain:AB Transcriptome or Gene expression PRJNA240316 Transcriptome Analysis Quantifying expression levels of smallRNAs between tissues in Danio Rerio strain AB.   pubmed:24835514     ZF1Brain1   strain:AB|age:Reproductive Adult|biomaterial provider:Postlethwait Lab Institute of Neuroscience 1254 University of Oregon 222 Huestis Hall Eugene OR 97403|sex:male|tissue:Brain|birth location:University of Oregon Fish Facility|collected by:Thomas Desvignes|collection date:2013 10 07|BioSampleModel:Model organism or animal                 Zebrafish Brain1 ZFBrain1 2 1 Tissue specific sequencing libraries were prepped and barcoded using the BiooScientific NEXTflex smallRNA Sequencing Kit which uses a 3’ adenylated adapter that ligates onto miRs and other small RNAs with a 3’ hydroxyl group.     miRNA-Seq TRANSCRIPTOMIC unspecified SINGLE ILLUMINA Illumina HiSeq 2500 <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>101</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> SRP039502     ZF1_Brain_3.fq.gz fastq 1192445794.0 11806394.0 Brain1 Run3 0:101 A:369786121;C:268557682;G:265557417;T:288006805;N:537769 101       369786121 268557682 265557417 288006805 537769 SRX685388 SRS566490 SRA142461 University of Oregon|JHP-Lab University of Oregon 1 2e-05   1e-05   1.0       101   T   under 1.2% mapping rate illumina hiseq_era unknown small_rna unknown bulk unknown unknown   United States 2014-08-22 Adult Adult Brain Nervous System
37929 37929 SRR1554493 SRX683354 SRS686645 SRP039502 PRJNA240316 Danio rerio strain:AB Transcriptome or Gene expression PRJNA240316 Transcriptome Analysis Quantifying expression levels of smallRNAs between tissues in Danio Rerio strain AB.   pubmed:24835514     ZF4Ovary1   strain:AB|age:Reproductive Adult|biomaterial provider:Postlethwait Lab Institute of Neuroscience 1254 University of Oregon 222 Huestis Hall Eugene OR 97410|sex:female|tissue:Ovary|birth location:University of Oregon Fish Facility|collected by:Thomas Desvignes|collection date:2013 10 07|BioSampleModel:Model organism or animal                 Zebrafish Ovary2 ZF4Ovary1 1 Tissue specific sequencing libraries were prepped and barcoded using the BiooScientific NEXTflex smallRNA Sequencing Kit which uses a 3’ adenylated adapter that ligates onto miRs and other small RNAs with a 3’ hydroxyl group.     miRNA-Seq TRANSCRIPTOMIC unspecified SINGLE ILLUMINA Illumina HiSeq 2500 <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>51</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> SRP039502     ZF4_Ovary_1.fq.gz fastq 476104278.0 9335378.0 Ovary2 Run1 0:51 A:111060704;C:120863975;G:129849761;T:114224677;N:105161 51       111060704 120863975 129849761 114224677 105161 SRX683354 SRS686645 SRA142461 University of Oregon|JHP-Lab University of Oregon 1 0.00027   8e-05   0.99945   0.8125   51   T   under 1.2% mapping rate illumina hiseq_era unknown small_rna unknown bulk unknown unknown   United States 2014-08-22 Adult Adult Gonad Reproductive System
37930 37930 SRR1554494 SRX683354 SRS686645 SRP039502 PRJNA240316 Danio rerio strain:AB Transcriptome or Gene expression PRJNA240316 Transcriptome Analysis Quantifying expression levels of smallRNAs between tissues in Danio Rerio strain AB.   pubmed:24835514     ZF4Ovary1   strain:AB|age:Reproductive Adult|biomaterial provider:Postlethwait Lab Institute of Neuroscience 1254 University of Oregon 222 Huestis Hall Eugene OR 97410|sex:female|tissue:Ovary|birth location:University of Oregon Fish Facility|collected by:Thomas Desvignes|collection date:2013 10 07|BioSampleModel:Model organism or animal                 Zebrafish Ovary2 ZF4Ovary1 1 Tissue specific sequencing libraries were prepped and barcoded using the BiooScientific NEXTflex smallRNA Sequencing Kit which uses a 3’ adenylated adapter that ligates onto miRs and other small RNAs with a 3’ hydroxyl group.     miRNA-Seq TRANSCRIPTOMIC unspecified SINGLE ILLUMINA Illumina HiSeq 2500 <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>51</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> SRP039502     ZF4_Ovary_2.fq.gz fastq 561956352.0 11018752.0 Ovary2 Run2 0:51 A:129925815;C:148479658;G:148478706;T:132824290;N:2247883 51       129925815 148479658 148478706 132824290 2247883 SRX683354 SRS686645 SRA142461 University of Oregon|JHP-Lab University of Oregon 1 0.00022   0.0001   0.99967   0.6875   51   T   under 1.2% mapping rate illumina hiseq_era unknown small_rna unknown bulk unknown unknown   United States 2014-08-22 Adult Adult Gonad Reproductive System
37931 37931 SRR1554490 SRX683353 SRS686644 SRP039502 PRJNA240316 Danio rerio strain:AB Transcriptome or Gene expression PRJNA240316 Transcriptome Analysis Quantifying expression levels of smallRNAs between tissues in Danio Rerio strain AB.   pubmed:24835514     ZF2Testis1   strain:AB|age:Reproductive Adult|biomaterial provider:Postlethwait Lab Institute of Neuroscience 1254 University of Oregon 222 Huestis Hall Eugene OR 97409|sex:male|tissue:Testis|birth location:University of Oregon Fish Facility|collected by:Thomas Desvignes|collection date:2013 10 07|BioSampleModel:Model organism or animal                 Zebrafish Testis2 ZF2Testis1 1 Tissue specific sequencing libraries were prepped and barcoded using the BiooScientific NEXTflex smallRNA Sequencing Kit which uses a 3’ adenylated adapter that ligates onto miRs and other small RNAs with a 3’ hydroxyl group.     miRNA-Seq TRANSCRIPTOMIC unspecified SINGLE ILLUMINA Illumina HiSeq 2500 <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>51</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> SRP039502     ZF2_Testis_1.fq.gz fastq 586152231.0 11493181.0 Testis2 Run1 0:51 A:145360493;C:148943988;G:151402108;T:140286245;N:159397 51       145360493 148943988 151402108 140286245 159397 SRX683353 SRS686644 SRA142461 University of Oregon|JHP-Lab University of Oregon 1 8e-05   2e-05   0.99985   0.57142   51   T   under 1.2% mapping rate illumina hiseq_era unknown small_rna unknown bulk unknown unknown   United States 2014-08-22 Adult Adult Gonad Reproductive System
37932 37932 SRR1554491 SRX683353 SRS686644 SRP039502 PRJNA240316 Danio rerio strain:AB Transcriptome or Gene expression PRJNA240316 Transcriptome Analysis Quantifying expression levels of smallRNAs between tissues in Danio Rerio strain AB.   pubmed:24835514     ZF2Testis1   strain:AB|age:Reproductive Adult|biomaterial provider:Postlethwait Lab Institute of Neuroscience 1254 University of Oregon 222 Huestis Hall Eugene OR 97409|sex:male|tissue:Testis|birth location:University of Oregon Fish Facility|collected by:Thomas Desvignes|collection date:2013 10 07|BioSampleModel:Model organism or animal                 Zebrafish Testis2 ZF2Testis1 1 Tissue specific sequencing libraries were prepped and barcoded using the BiooScientific NEXTflex smallRNA Sequencing Kit which uses a 3’ adenylated adapter that ligates onto miRs and other small RNAs with a 3’ hydroxyl group.     miRNA-Seq TRANSCRIPTOMIC unspecified SINGLE ILLUMINA Illumina HiSeq 2500 <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>51</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> SRP039502     ZF2_Testis_2.fq.gz fastq 697016337.0 13666987.0 Testis2 Run2 0:51 A:172082858;C:184634425;G:173370430;T:163527914;N:3400710 51       172082858 184634425 173370430 163527914 3400710 SRX683353 SRS686644 SRA142461 University of Oregon|JHP-Lab University of Oregon 1 8e-05   2e-05   0.99985   0.57142   51   T   under 1.2% mapping rate illumina hiseq_era unknown small_rna unknown bulk unknown unknown   United States 2014-08-22 Adult Adult Gonad Reproductive System
37933 37933 SRR1554487 SRX683351 SRS686642 SRP039502 PRJNA240316 Danio rerio strain:AB Transcriptome or Gene expression PRJNA240316 Transcriptome Analysis Quantifying expression levels of smallRNAs between tissues in Danio Rerio strain AB.   pubmed:24835514     ZF2Heart1   strain:AB|age:Reproductive Adult|biomaterial provider:Postlethwait Lab Institute of Neuroscience 1254 University of Oregon 222 Huestis Hall Eugene OR 97408|sex:male|tissue:Heart|birth location:University of Oregon Fish Facility|collected by:Thomas Desvignes|collection date:2013 10 07|BioSampleModel:Model organism or animal                 Zebrafish Heart2 ZF2Heart1 1 Tissue specific sequencing libraries were prepped and barcoded using the BiooScientific NEXTflex smallRNA Sequencing Kit which uses a 3’ adenylated adapter that ligates onto miRs and other small RNAs with a 3’ hydroxyl group.     miRNA-Seq TRANSCRIPTOMIC unspecified SINGLE ILLUMINA Illumina HiSeq 2500 <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>51</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> SRP039502     ZF2_Heart_1.fq.gz fastq 767836212.0 15055612.0 Heart2 Run1 0:51 A:192620363;C:202013249;G:191038597;T:181862075;N:301928 51       192620363 202013249 191038597 181862075 301928 SRX683351 SRS686642 SRA142461 University of Oregon|JHP-Lab University of Oregon 1 5e-05   3e-05   0.99995   1.0   51   T   under 1.2% mapping rate illumina hiseq_era unknown small_rna unknown bulk unknown unknown   United States 2014-08-22 Adult Adult Heart Cardiovascular System
37934 37934 SRR1554488 SRX683351 SRS686642 SRP039502 PRJNA240316 Danio rerio strain:AB Transcriptome or Gene expression PRJNA240316 Transcriptome Analysis Quantifying expression levels of smallRNAs between tissues in Danio Rerio strain AB.   pubmed:24835514     ZF2Heart1   strain:AB|age:Reproductive Adult|biomaterial provider:Postlethwait Lab Institute of Neuroscience 1254 University of Oregon 222 Huestis Hall Eugene OR 97408|sex:male|tissue:Heart|birth location:University of Oregon Fish Facility|collected by:Thomas Desvignes|collection date:2013 10 07|BioSampleModel:Model organism or animal                 Zebrafish Heart2 ZF2Heart1 1 Tissue specific sequencing libraries were prepped and barcoded using the BiooScientific NEXTflex smallRNA Sequencing Kit which uses a 3’ adenylated adapter that ligates onto miRs and other small RNAs with a 3’ hydroxyl group.     miRNA-Seq TRANSCRIPTOMIC unspecified SINGLE ILLUMINA Illumina HiSeq 2500 <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>51</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> SRP039502     ZF2_Heart_2.fq.gz fastq 979886205.0 19213455.0 Heart2 Run2 0:51 A:246169086;C:264387065;G:236377487;T:227289693;N:5662874 51       246169086 264387065 236377487 227289693 5662874 SRX683351 SRS686642 SRA142461 University of Oregon|JHP-Lab University of Oregon 1 2e-05   0.0   1.0       51   T   under 1.2% mapping rate illumina hiseq_era unknown small_rna unknown bulk unknown unknown   United States 2014-08-22 Adult Adult Heart Cardiovascular System

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CREATE TABLE run_metadata("run.accession" VARCHAR, "experiment.accession" VARCHAR, "sample.accession" VARCHAR, "study.accession" VARCHAR, bioproject VARCHAR, "study.title" VARCHAR, "study.alias" VARCHAR, "study.type" VARCHAR, "study.abstract" VARCHAR, "study.attributes" VARCHAR, "study.PMIDs" VARCHAR, "sample.description" VARCHAR, "sample.title" VARCHAR, "sample.alias" VARCHAR, "sample.centername" VARCHAR, "sample.attributes" VARCHAR, "GEOsample.title" VARCHAR, "GEOsample.dataprocessing" VARCHAR, "GEOsample.source" VARCHAR, "GEOsample.treatmentprotocol" VARCHAR, "GEOsample.extractprotocol" VARCHAR, "GEOsample.growthprotocol" VARCHAR, "GEOsample.characteristics" VARCHAR, "GEOsample.accession" VARCHAR, "experiment.title" VARCHAR, "experiment.alias" VARCHAR, "experiment.library_name" VARCHAR, "experiment.design_description" VARCHAR, "experiment.library_construction_protocol" VARCHAR, "experiment.attributes" VARCHAR, "experiment.library_strategy" VARCHAR, "experiment.library_source" VARCHAR, "experiment.library_selection" VARCHAR, "experiment.library_layout" VARCHAR, "experiment.platform" VARCHAR, "experiment.instrument_model" VARCHAR, "experiment.spot_descriptor" VARCHAR, "experiment.study_ref" VARCHAR, "run.title" VARCHAR, "run.attributes" VARCHAR, "run.filename" VARCHAR, "run.semantic_name" VARCHAR, "run.total_bases" DOUBLE, "run.total_spots" DOUBLE, "run.alias" VARCHAR, "run.read_lengths" VARCHAR, "run.base_counts" VARCHAR, "run.r1_length" BIGINT, "run.r2_length" BIGINT, "run.r3_length" BIGINT, "run.r4_length" BIGINT, "run.Acount" BIGINT, "run.Ccount" BIGINT, "run.Gcount" BIGINT, "run.Tcount" BIGINT, "run.Ncount" BIGINT, "run.experiment" VARCHAR, "run.pool_member" VARCHAR, "submission.accession" VARCHAR, "submission.srasource" VARCHAR, "submission.bioprojectsource" VARCHAR, "seqdetective.n_mates" BIGINT, "seqdetective.mapping_rate.mate1" DOUBLE, "seqdetective.mapping_rate.mate2" DOUBLE, "seqdetective.nofeature_rate.mate1" DOUBLE, "seqdetective.nofeature_rate.mate2" DOUBLE, "seqdetective.sparsity.mate1" DOUBLE, "seqdetective.sparsity.mate2" DOUBLE, "seqdetective.pos_strand_rate.mate1" DOUBLE, "seqdetective.pos_strand_rate.mate2" DOUBLE, "seqdetective.readlen.mate1" BIGINT, "seqdetective.readlen.mate2" BIGINT, "seqdetective.judgement.mate1" VARCHAR, "seqdetective.judgement.mate2" VARCHAR, "seqdetective.judgement.reason" VARCHAR, platform_family VARCHAR, instrument_generation VARCHAR, read_bias VARCHAR, selection_class VARCHAR, prep_kit VARCHAR, sc_or_bulk VARCHAR, tech_class VARCHAR, technology VARCHAR, tech_variant VARCHAR, "submission.bioprojectsource.country" VARCHAR, earliest_date DATE, devstage_curation VARCHAR, devstage_curation_coarse VARCHAR, tissue_curation VARCHAR, tissue_curation_coarse VARCHAR);;
CREATE INDEX idx_run_bioproject ON run_metadata(bioproject);;
CREATE INDEX idx_run_run_accession ON run_metadata("run.accession");;