run_metadata
13 rows where experiment.library_layout = "SINGLE", experiment.library_selection = "RT-PCR" and tissue_curation = "Liver"
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| Link | rowid ▼ | run.accession | experiment.accession | sample.accession | study.accession | bioproject | study.title | study.alias | study.type | study.abstract | study.attributes | study.PMIDs | sample.description | sample.title | sample.alias | sample.centername | sample.attributes | GEOsample.title | GEOsample.dataprocessing | GEOsample.source | GEOsample.treatmentprotocol | GEOsample.extractprotocol | GEOsample.growthprotocol | GEOsample.characteristics | GEOsample.accession | experiment.title | experiment.alias | experiment.library_name | experiment.design_description | experiment.library_construction_protocol | experiment.attributes | experiment.library_strategy | experiment.library_source | experiment.library_selection | experiment.library_layout | experiment.platform | experiment.instrument_model | experiment.spot_descriptor | experiment.study_ref | run.title | run.attributes | run.filename | run.semantic_name | run.total_bases | run.total_spots | run.alias | run.read_lengths | run.base_counts | run.r1_length | run.r2_length | run.r3_length | run.r4_length | run.Acount | run.Ccount | run.Gcount | run.Tcount | run.Ncount | run.experiment | run.pool_member | submission.accession | submission.srasource | submission.bioprojectsource | seqdetective.n_mates | seqdetective.mapping_rate.mate1 | seqdetective.mapping_rate.mate2 | seqdetective.nofeature_rate.mate1 | seqdetective.nofeature_rate.mate2 | seqdetective.sparsity.mate1 | seqdetective.sparsity.mate2 | seqdetective.pos_strand_rate.mate1 | seqdetective.pos_strand_rate.mate2 | seqdetective.readlen.mate1 | seqdetective.readlen.mate2 | seqdetective.judgement.mate1 | seqdetective.judgement.mate2 | seqdetective.judgement.reason | platform_family | instrument_generation | read_bias | selection_class | prep_kit | sc_or_bulk | tech_class | technology | tech_variant | submission.bioprojectsource.country | earliest_date | devstage_curation | devstage_curation_coarse | tissue_curation | tissue_curation_coarse |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 38289 | 38289 | SRR1731761 | SRX828260 | SRS807370 | SRP051449 | PRJNA266270 | Danio rerio Transcriptome or Gene expression | PRJNA266270 | Other | To identify molecular signals that initiate liver regeneration post 1/3 PH | sham operated liver transcriptome post 1/3 PH | breed:AB line|cultivar:AB line|strain:AB line|age:10 month|sex:female|tissue:liver|BioSampleModel:Model organism or animal | sham operated liver transcriptome post 1/3 PH | sham operated | 1 | 1 | RNA-Seq | TRANSCRIPTOMIC | RT-PCR | SINGLE | ILLUMINA | Illumina Genome Analyzer IIx | <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>72</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> | SRP051449 | sham-operated_R1.fastq.gz sham-operated_R2.fastq.gz | fastq fastq | 2339904960.0 | 16249340.0 | sham operated | 0:72 1:72 | A:639730953;C:549099184;G:603489762;T:545135156;N:2449905 | 72 | 72 | 639730953 | 549099184 | 603489762 | 545135156 | 2449905 | SRX828260 | SRS807370 | SRA223828 | Institute of Hydrobiology, Chinese Academy of Scie|The Key Laboratory of Aquatic Biodiversity and Con | Chinese Academy of Sciences | 2 | 0.9353 | 0.91074 | 0.02617 | 0.0253 | 0.86334 | 0.86387 | 0.35902 | 0.36066 | 72 | 72 | B | B | biological fallback assumption | illumina | early_illumina | unknown | other | unknown | bulk | unknown | unknown | China | 2016-02-05 | Adult | Adult | Liver | Liver and Biliary System | ||||||||||||||||||||
| 42181 | 42181 | SRR5482153 | SRX2765568 | SRS2149771 | SRP105243 | PRJNA383986 | Danio rerio Raw sequence reads | PRJNA383986 | Other | The goal of the experiment was to simulate the effect of size selective harvesting and captive rearing on gene expression in a controlled environment using zebrafish as a model species. | Danio rerio: Large harvested line biological replicate 1 F2 offspring originating from wild caught population | LS1 F2 | breed:missing|dev stage:juvenile|sex:female|tissue:liver|death date:2008|BioSampleModel:Model organism or animal | RNAseq of Danio rerio: juvenile female liver RNA of 4 biological replicates from a large harvested selection line F2 individuals | LS1 F2 | LS1 F2 | Total RNA from liver tissue of juvenile zebrafish was extracted according to the Trizol method with TRI reagent Sigma. The liver tissue was homogenized with a TissueLyser II homogenizer Qiagen. DNase treatment was performed to remove any genomic DNA from the samples. RNA was eluted in RNase free water and RNA concentrations and the integrity of RNA samples were determined using an Agilent 2100 Bioanalyzer. Total RNA was processed by using Low Input Library Preparation Kit to prepare the RNA Seq library. | RNA-Seq | TRANSCRIPTOMIC | RT-PCR | SINGLE | ILLUMINA | Illumina HiSeq 2000 | SRP105243 | LS1-F2-10_FCC4CMHACXX_L1_WHZEBagiNCADRAAPEI-205_1.fq.gz LS1-F2-1_FCC4BKHACXX_L7_WHZEBagiNCAARAAPEI-201_1.fq.gz LS1-F2-5_FCC4BKHACXX_L7_WHZEBagiNCABRAAPEI-202_1.fq.gz LS1-F2-8_FCC4BKHACXX_L7_WHZEBagiNCACRAAPEI-203_1.fq.gz | fastq fastq fastq fastq | 12496905120.0 | 255038880.0 | LS1 F2 10 FCC4CMHACXX L1 WHZEBagiNCADRAAPEI 205 1.fq.gz | 0:49 | A:3275093505;C:2981786839;G:2904373605;T:3334855308;N:795863 | 49 | 3275093505 | 2981786839 | 2904373605 | 3334855308 | 795863 | SRX2765568 | SRS2149771 | SRA557107 | University of Turku|Biology | University of Turku | 1 | 0.88577 | 0.04599 | 0.76469 | 0.48703 | 49 | B | usable mapping rate | illumina | hiseq_era | unknown | other | unknown | bulk | unknown | unknown | Finland | 2017-12-04 | Juvenile | Juvenile | Liver | Liver and Biliary System | |||||||||||||||||||||||||||
| 42182 | 42182 | SRR5482152 | SRX2765567 | SRS2149770 | SRP105243 | PRJNA383986 | Danio rerio Raw sequence reads | PRJNA383986 | Other | The goal of the experiment was to simulate the effect of size selective harvesting and captive rearing on gene expression in a controlled environment using zebrafish as a model species. | Danio rerio: Large harvested line biological replicate 2 F2 offspring originating from wild caught population | LS2 F2 | breed:missing|dev stage:juvenile|sex:female|tissue:liver|death date:2008|BioSampleModel:Model organism or animal | RNAseq of Danio rerio: juvenile female liver RNA of 4 biological replicates from a large harvested selection line F2 individuals | LS2 F2 | LS2 F2 | Total RNA from liver tissue of juvenile zebrafish was extracted according to the Trizol method with TRI reagent Sigma. The liver tissue was homogenized with a TissueLyser II homogenizer Qiagen. DNase treatment was performed to remove any genomic DNA from the samples. RNA was eluted in RNase free water and RNA concentrations and the integrity of RNA samples were determined using an Agilent 2100 Bioanalyzer. Total RNA was processed by using Low Input Library Preparation Kit to prepare the RNA Seq library. | RNA-Seq | TRANSCRIPTOMIC | RT-PCR | SINGLE | ILLUMINA | Illumina HiSeq 2000 | SRP105243 | LS2-F2-10_FCC4CMHACXX_L4_WHZEBagiNCALRAAPEI-213_1.fq.gz LS2-F2-11_FCC4CMHACXX_L4_WHZEBagiNCAMRAAPEI-214_1.fq.gz LS2-F2-14_FCC4C2AACXX_L1_WHZEBagiNCANRAAPEI-215_1.fq.gz LS2-F2-9_FCC4CMHACXX_L4_WHZEBagiNCAKRAAPEI-212_1.fq.gz | fastq fastq fastq fastq | 11108547205.0 | 226705045.0 | LS2 F2 9 FCC4CMHACXX L4 WHZEBagiNCAKRAAPEI 212 1.fq.gz | 0:49 | A:2930693084;C:2628276474;G:2564131315;T:2984423047;N:1023285 | 49 | 2930693084 | 2628276474 | 2564131315 | 2984423047 | 1023285 | SRX2765567 | SRS2149770 | SRA557107 | University of Turku|Biology | University of Turku | 1 | 0.90013 | 0.04677 | 0.81251 | 0.44762 | 49 | B | usable mapping rate | illumina | hiseq_era | unknown | other | unknown | bulk | unknown | unknown | Finland | 2017-12-04 | Juvenile | Juvenile | Liver | Liver and Biliary System | |||||||||||||||||||||||||||
| 42183 | 42183 | SRR5482151 | SRX2765565 | SRS2149769 | SRP105243 | PRJNA383986 | Danio rerio Raw sequence reads | PRJNA383986 | Other | The goal of the experiment was to simulate the effect of size selective harvesting and captive rearing on gene expression in a controlled environment using zebrafish as a model species. | Danio rerio: Random harvested line biological replicate 1 F2 offspring originating from wild caught population | RS1 F2 | breed:missing|dev stage:juvenile|sex:female|tissue:liver|death date:2008|BioSampleModel:Model organism or animal | RNAseq of Danio rerio: juvenile female liver RNA of 4 biological replicates from a random harvested selection line F2 individuals | RS1 F2 | RS1 F2 | Total RNA from liver tissue of juvenile zebrafish was extracted according to the Trizol method with TRI reagent Sigma. The liver tissue was homogenized with a TissueLyser II homogenizer Qiagen. DNase treatment was performed to remove any genomic DNA from the samples. RNA was eluted in RNase free water and RNA concentrations and the integrity of RNA samples were determined using an Agilent 2100 Bioanalyzer. Total RNA was processed by using Low Input Library Preparation Kit to prepare the RNA Seq library. | RNA-Seq | TRANSCRIPTOMIC | RT-PCR | SINGLE | ILLUMINA | Illumina HiSeq 2000 | SRP105243 | RS1-F2-14_FCC4C2AACXX_L2_WHZEBagiNCAURAAPEI-223_1.fq.gz RS1-F2-16_FCC4C2AACXX_L2_WHZEBagiNCAVRAAPEI-225_1.fq.gz RS1-F2-17_FCC4C2AACXX_L3_WHZEBagiNCAWRAAPEI-227_1.fq.gz RS1-F2-19_FCC4C2AACXX_L3_WHZEBagiNCAXRAAPEI-201_1.fq.gz | fastq fastq fastq fastq | 12074501894.0 | 246418406.0 | RS1 F2 16 FCC4C2AACXX L2 WHZEBagiNCAVRAAPEI 225 1.fq.gz | 0:49 | A:3199737416;C:2851335695;G:2767557430;T:3254746975;N:1124378 | 49 | 3199737416 | 2851335695 | 2767557430 | 3254746975 | 1124378 | SRX2765565 | SRS2149769 | SRA557107 | University of Turku|Biology | University of Turku | 1 | 0.87838 | 0.05071 | 0.81663 | 0.55417 | 49 | B | usable mapping rate | illumina | hiseq_era | unknown | other | unknown | bulk | unknown | unknown | Finland | 2017-12-04 | Juvenile | Juvenile | Liver | Liver and Biliary System | |||||||||||||||||||||||||||
| 42184 | 42184 | SRR5482150 | SRX2765564 | SRS2149768 | SRP105243 | PRJNA383986 | Danio rerio Raw sequence reads | PRJNA383986 | Other | The goal of the experiment was to simulate the effect of size selective harvesting and captive rearing on gene expression in a controlled environment using zebrafish as a model species. | Danio rerio: Random harvested line biological replicate 2 F2 offspring originating from wild caught population | RS2 F2 | breed:missing|dev stage:juvenile|sex:female|tissue:liver|death date:2008|BioSampleModel:Model organism or animal | RNAseq of Danio rerio: juvenile female liver RNA of 4 biological replicates from a random harvested selection line F2 individuals | RS2 F2 | RS2 F2 | Total RNA from liver tissue of juvenile zebrafish was extracted according to the Trizol method with TRI reagent Sigma. The liver tissue was homogenized with a TissueLyser II homogenizer Qiagen. DNase treatment was performed to remove any genomic DNA from the samples. RNA was eluted in RNase free water and RNA concentrations and the integrity of RNA samples were determined using an Agilent 2100 Bioanalyzer. Total RNA was processed by using Low Input Library Preparation Kit to prepare the RNA Seq library. | RNA-Seq | TRANSCRIPTOMIC | RT-PCR | SINGLE | ILLUMINA | Illumina HiSeq 2000 | SRP105243 | RS2-F2-3_FCC4CMHACXX_L2_WHZEBagiNCBFRAAPEI-210_1.fq.gz RS2-F2-6-2_FCC4CMHACXX_L2_WHZEBagiNCBGRAAPEI-211_1.fq.gz RS2-F2-8_FCC4CMHACXX_L2_WHZEBagiNCBHRABPEI-212_1.fq.gz RS2-F2-9_FCC4CMHACXX_L3_WHZEBagiNCBIRABPEI-213_1.fq.gz | fastq fastq fastq fastq | 11923597525.0 | 243338725.0 | RS2 F2 9 FCC4CMHACXX L3 WHZEBagiNCBIRABPEI 213 1.fq.gz | 0:49 | A:3132507165;C:2819390547;G:2776793842;T:3193588286;N:1317685 | 49 | 3132507165 | 2819390547 | 2776793842 | 3193588286 | 1317685 | SRX2765564 | SRS2149768 | SRA557107 | University of Turku|Biology | University of Turku | 1 | 0.90986 | 0.05269 | 0.814 | 0.36827 | 49 | B | usable mapping rate | illumina | hiseq_era | unknown | other | unknown | bulk | unknown | unknown | Finland | 2017-12-04 | Juvenile | Juvenile | Liver | Liver and Biliary System | |||||||||||||||||||||||||||
| 42185 | 42185 | SRR5482149 | SRX2765563 | SRS2149767 | SRP105243 | PRJNA383986 | Danio rerio Raw sequence reads | PRJNA383986 | Other | The goal of the experiment was to simulate the effect of size selective harvesting and captive rearing on gene expression in a controlled environment using zebrafish as a model species. | Danio rerio: Large harvested line biological replicate 1 F5 offspring originating from wild caught population | LS1 F5 | breed:missing|dev stage:juvenile|sex:female|tissue:liver|death date:2010|BioSampleModel:Model organism or animal | RNAseq of Danio rerio: juvenile female liver RNA of 4 biological replicates from a large harvested selection line F5 individuals | LS1 F5 | LS1 F5 | Total RNA from liver tissue of juvenile zebrafish was extracted according to the Trizol method with TRI reagent Sigma. The liver tissue was homogenized with a TissueLyser II homogenizer Qiagen. DNase treatment was performed to remove any genomic DNA from the samples. RNA was eluted in RNase free water and RNA concentrations and the integrity of RNA samples were determined using an Agilent 2100 Bioanalyzer. Total RNA was processed by using Low Input Library Preparation Kit to prepare the RNA Seq library. | RNA-Seq | TRANSCRIPTOMIC | RT-PCR | SINGLE | ILLUMINA | Illumina HiSeq 2000 | SRP105243 | LS1-15_FCC4C3HACXX_L1_WHZEBagiNBANRAAPEI-215_1.fq.gz LS1-2_FCC4BJ2ACXX_L5_WHZEBagiNBABRAAPEI-202_1.fq.gz LS1-6_FCC4BJ2ACXX_L6_WHZEBagiNBAFRAAPEI-207_1.fq.gz LS1-8_FCC4BJ2ACXX_L8_WHZEBagiNBAJRAAPEI-211_1.fq.gz | fastq fastq fastq fastq | 12776192331.0 | 260738619.0 | LS1 2 FCC4BJ2ACXX L5 WHZEBagiNBABRAAPEI 202 1.fq.gz | 0:49 | A:3371838433;C:3025006624;G:2934135898;T:3444117388;N:1093988 | 49 | 3371838433 | 3025006624 | 2934135898 | 3444117388 | 1093988 | SRX2765563 | SRS2149767 | SRA557107 | University of Turku|Biology | University of Turku | 1 | 0.88482 | 0.04932 | 0.813 | 0.51224 | 49 | B | usable mapping rate | illumina | hiseq_era | unknown | other | unknown | bulk | unknown | unknown | Finland | 2017-12-04 | Juvenile | Juvenile | Liver | Liver and Biliary System | |||||||||||||||||||||||||||
| 42186 | 42186 | SRR5482148 | SRX2765562 | SRS2149766 | SRP105243 | PRJNA383986 | Danio rerio Raw sequence reads | PRJNA383986 | Other | The goal of the experiment was to simulate the effect of size selective harvesting and captive rearing on gene expression in a controlled environment using zebrafish as a model species. | Danio rerio: Large harvested line biological replicate 2 F5 offspring originating from wild caught population | LS2 F5 | breed:missing|dev stage:juvenile|sex:female|tissue:liver|death date:2010|BioSampleModel:Model organism or animal | RNAseq of Danio rerio: juvenile female liver RNA of 4 biological replicates from a large harvested selection line F5 individuals | LS2 F5 | LS2 F5 | Total RNA from liver tissue of juvenile zebrafish was extracted according to the Trizol method with TRI reagent Sigma. The liver tissue was homogenized with a TissueLyser II homogenizer Qiagen. DNase treatment was performed to remove any genomic DNA from the samples. RNA was eluted in RNase free water and RNA concentrations and the integrity of RNA samples were determined using an Agilent 2100 Bioanalyzer. Total RNA was processed by using Low Input Library Preparation Kit to prepare the RNA Seq library. | RNA-Seq | TRANSCRIPTOMIC | RT-PCR | SINGLE | ILLUMINA | Illumina HiSeq 2000 | SRP105243 | LS2-10_FCC4BJ2ACXX_L8_WHZEBagiNBAKRAAPEI-212_1.fq.gz LS2-11_FCC4C3HACXX_L1_WHZEBagiNBAORAAPEI-216_1.fq.gz LS2-1_FCC4BJ2ACXX_L5_WHZEBagiNBACRAAPEI-203_1.fq.gz LS2-7_FCC4BVYACXX_L1_WHZEBagiNBAGRAAPEI-208_1.fq.gz | fastq fastq fastq fastq | 11941143935.0 | 243696815.0 | LS2 10 FCC4BJ2ACXX L8 WHZEBagiNBAKRAAPEI 212 1.fq.gz | 0:49 | A:3165540142;C:2817777586;G:2733117846;T:3223679054;N:1029307 | 49 | 3165540142 | 2817777586 | 2733117846 | 3223679054 | 1029307 | SRX2765562 | SRS2149766 | SRA557107 | University of Turku|Biology | University of Turku | 1 | 0.87119 | 0.04752 | 0.80251 | 0.55444 | 49 | B | usable mapping rate | illumina | hiseq_era | unknown | other | unknown | bulk | unknown | unknown | Finland | 2017-12-04 | Juvenile | Juvenile | Liver | Liver and Biliary System | |||||||||||||||||||||||||||
| 42187 | 42187 | SRR5482147 | SRX2765561 | SRS2149765 | SRP105243 | PRJNA383986 | Danio rerio Raw sequence reads | PRJNA383986 | Other | The goal of the experiment was to simulate the effect of size selective harvesting and captive rearing on gene expression in a controlled environment using zebrafish as a model species. | Danio rerio: Random harvested line biological replicate 1 F5 offspring originating from wild caught population | RS1 F5 | breed:missing|dev stage:juvenile|sex:female|tissue:liver|death date:2010|BioSampleModel:Model organism or animal | RNAseq of Danio rerio: juvenile female liver RNA of 4 biological replicates from a random harvested selection line F5 individuals | RS1 F5 | RS1 F5 | Total RNA from liver tissue of juvenile zebrafish was extracted according to the Trizol method with TRI reagent Sigma. The liver tissue was homogenized with a TissueLyser II homogenizer Qiagen. DNase treatment was performed to remove any genomic DNA from the samples. RNA was eluted in RNase free water and RNA concentrations and the integrity of RNA samples were determined using an Agilent 2100 Bioanalyzer. Total RNA was processed by using Low Input Library Preparation Kit to prepare the RNA Seq library. | RNA-Seq | TRANSCRIPTOMIC | RT-PCR | SINGLE | ILLUMINA | Illumina HiSeq 2000 | SRP105243 | RS1-10_FCC4BJ2ACXX_L8_WHZEBagiNBALRAAPEI-213_1.fq.gz RS1-2_FCC4Y2GACXX_L4_WHZEBagiNCBPRABPEI-221_1.fq.gz RS1-3_FCC4BJ2ACXX_L6_WHZEBagiNBADRAAPEI-205_1.fq.gz RS1-6_FCC4BV7ACXX_L1_WHZEBagiNBAHRAAPEI-209_1.fq.gz | fastq fastq fastq fastq | 12149652753.0 | 247952097.0 | RS1 6 FCC4BV7ACXX L1 WHZEBagiNBAHRAAPEI 209 1.fq.gz | 0:49 | A:3177681868;C:2907323525;G:2822484622;T:3241144133;N:1018605 | 49 | 3177681868 | 2907323525 | 2822484622 | 3241144133 | 1018605 | SRX2765561 | SRS2149765 | SRA557107 | University of Turku|Biology | University of Turku | 1 | 0.90495 | 0.04625 | 0.80028 | 0.56443 | 49 | B | usable mapping rate | illumina | hiseq_era | unknown | other | unknown | bulk | unknown | unknown | Finland | 2017-12-04 | Juvenile | Juvenile | Liver | Liver and Biliary System | |||||||||||||||||||||||||||
| 42188 | 42188 | SRR5482146 | SRX2765560 | SRS2149764 | SRP105243 | PRJNA383986 | Danio rerio Raw sequence reads | PRJNA383986 | Other | The goal of the experiment was to simulate the effect of size selective harvesting and captive rearing on gene expression in a controlled environment using zebrafish as a model species. | Danio rerio: Random harvested line biological replicate 2 F5 offspring originating from wild caught population | RS2 F5 | breed:missing|dev stage:juvenile|sex:female|tissue:liver|death date:2010|BioSampleModel:Model organism or animal | RNAseq of Danio rerio: juvenile female liver RNA of 4 biological replicates from a random harvested selection line F5 individuals | RS2 F5 | RS2 F5 | Total RNA from liver tissue of juvenile zebrafish was extracted according to the Trizol method with TRI reagent Sigma. The liver tissue was homogenized with a TissueLyser II homogenizer Qiagen. DNase treatment was performed to remove any genomic DNA from the samples. RNA was eluted in RNase free water and RNA concentrations and the integrity of RNA samples were determined using an Agilent 2100 Bioanalyzer. Total RNA was processed by using Low Input Library Preparation Kit to prepare the RNA Seq library. | RNA-Seq | TRANSCRIPTOMIC | RT-PCR | SINGLE | ILLUMINA | Illumina HiSeq 2000 | SRP105243 | RS2-10_FCC4BVYACXX_L1_WHZEBagiNBAIRAAPEI-210_1.fq.gz RS2-13_FCC4C3HACXX_L1_WHZEBagiNBAMRAAPEI-214_1.fq.gz RS2-2_FCC4BJ2ACXX_L5_WHZEBagiNBAARAAPEI-201_1.fq.gz RS2-3_FCC4C19ACXX_L5_WHZEBagiNBAERAAPEI-206_1.fq.gz | fastq fastq fastq fastq | 11262796951.0 | 229852999.0 | RS2 13 FCC4C3HACXX L1 WHZEBagiNBAMRAAPEI 214 1.fq.gz | 0:49 | A:2931626627;C:2710523895;G:2627211912;T:2992545637;N:888880 | 49 | 2931626627 | 2710523895 | 2627211912 | 2992545637 | 888880 | SRX2765560 | SRS2149764 | SRA557107 | University of Turku|Biology | University of Turku | 1 | 0.88588 | 0.04266 | 0.8112 | 0.52927 | 49 | B | usable mapping rate | illumina | hiseq_era | unknown | other | unknown | bulk | unknown | unknown | Finland | 2017-12-04 | Juvenile | Juvenile | Liver | Liver and Biliary System | |||||||||||||||||||||||||||
| 42189 | 42189 | SRR5482145 | SRX2765559 | SRS2149763 | SRP105243 | PRJNA383986 | Danio rerio Raw sequence reads | PRJNA383986 | Other | The goal of the experiment was to simulate the effect of size selective harvesting and captive rearing on gene expression in a controlled environment using zebrafish as a model species. | Danio rerio: Large harvested line biological replicate 1 F11 offspring originating from wild caught population | LS1 F11 | breed:missing|dev stage:juvenile|sex:female|tissue:liver|death date:2014|BioSampleModel:Model organism or animal | RNAseq of Danio rerio: juvenile female liver RNA of 4 biological replicates from a large harvested selection line F11 individuals | LS1 F11 | LS1 F11 | Total RNA from liver tissue of juvenile zebrafish was extracted according to the Trizol method with TRI reagent Sigma. The liver tissue was homogenized with a TissueLyser II homogenizer Qiagen. DNase treatment was performed to remove any genomic DNA from the samples. RNA was eluted in RNase free water and RNA concentrations and the integrity of RNA samples were determined using an Agilent 2100 Bioanalyzer. Total RNA was processed by using Low Input Library Preparation Kit to prepare the RNA Seq library. | RNA-Seq | TRANSCRIPTOMIC | RT-PCR | SINGLE | ILLUMINA | Illumina HiSeq 2000 | SRP105243 | LS1-F11B-11_FCC4CMHACXX_L1_WHZEBagiNCAERAAPEI-206_1.fq.gz LS1-F11B-13_FCC4CMHACXX_L1_WHZEBagiNCAFRAAPEI-207_1.fq.gz LS1-F11B-16_FCC4C19ACXX_L1_WHZEBagiNCAGRBAPEI-208_1.fq.gz LS1-F11B-17_FCC4BKHACXX_L8_WHZEBagiNCAHRAAPEI-209_1.fq.gz | fastq fastq fastq fastq | 11326213584.0 | 231147216.0 | LS1 F11B 16 FCC4C19ACXX L1 WHZEBagiNCAGRBAPEI 208 1.fq.gz | 0:49 | A:2982223592;C:2693407623;G:2622876703;T:3026833843;N:871823 | 49 | 2982223592 | 2693407623 | 2622876703 | 3026833843 | 871823 | SRX2765559 | SRS2149763 | SRA557107 | University of Turku|Biology | University of Turku | 1 | 0.90202 | 0.04273 | 0.78273 | 0.51238 | 49 | B | usable mapping rate | illumina | hiseq_era | unknown | other | unknown | bulk | unknown | unknown | Finland | 2017-12-04 | Juvenile | Juvenile | Liver | Liver and Biliary System | |||||||||||||||||||||||||||
| 42190 | 42190 | SRR5482144 | SRX2765558 | SRS2149762 | SRP105243 | PRJNA383986 | Danio rerio Raw sequence reads | PRJNA383986 | Other | The goal of the experiment was to simulate the effect of size selective harvesting and captive rearing on gene expression in a controlled environment using zebrafish as a model species. | Danio rerio: Large harvested line biological replicate 2 F11 offspring originating from wild caught population | LS2 F11 | breed:missing|dev stage:juvenile|sex:female|tissue:liver|death date:2014|BioSampleModel:Model organism or animal | RNAseq of Danio rerio: juvenile female liver RNA of 4 biological replicates from a large harvested selection line F11 individuals | LS2 F11 | LS2 F11 | Total RNA from liver tissue of juvenile zebrafish was extracted according to the Trizol method with TRI reagent Sigma. The liver tissue was homogenized with a TissueLyser II homogenizer Qiagen. DNase treatment was performed to remove any genomic DNA from the samples. RNA was eluted in RNase free water and RNA concentrations and the integrity of RNA samples were determined using an Agilent 2100 Bioanalyzer. Total RNA was processed by using Low Input Library Preparation Kit to prepare the RNA Seq library. | RNA-Seq | TRANSCRIPTOMIC | RT-PCR | SINGLE | ILLUMINA | Illumina HiSeq 2000 | SRP105243 | LS2-F11B-1_FCC4C2AACXX_L1_WHZEBagiNCAORAAPEI-216_1.fq.gz LS2-F11B-2_FCC4C2AACXX_L1_WHZEBagiNCAPRAAPEI-218_1.fq.gz LS2-F11B-5_FCC4CMHACXX_L5_WHZEBagiNCAQRAAPEI-219_1.fq.gz LS2-F11B-7_FCC4CMHACXX_L5_WHZEBagiNCARRAAPEI-220_1.fq.gz | fastq fastq fastq fastq | 12030571875.0 | 245521875.0 | LS2 F11B 7 FCC4CMHACXX L5 WHZEBagiNCARRAAPEI 220 1.fq.gz | 0:49 | A:3149792862;C:2871072895;G:2795019949;T:3213514581;N:1171588 | 49 | 3149792862 | 2871072895 | 2795019949 | 3213514581 | 1171588 | SRX2765558 | SRS2149762 | SRA557107 | University of Turku|Biology | University of Turku | 1 | 0.91893 | 0.036 | 0.77429 | 0.38962 | 49 | B | usable mapping rate | illumina | hiseq_era | unknown | other | unknown | bulk | unknown | unknown | Finland | 2017-12-04 | Juvenile | Juvenile | Liver | Liver and Biliary System | |||||||||||||||||||||||||||
| 42191 | 42191 | SRR5482143 | SRX2765557 | SRS2149760 | SRP105243 | PRJNA383986 | Danio rerio Raw sequence reads | PRJNA383986 | Other | The goal of the experiment was to simulate the effect of size selective harvesting and captive rearing on gene expression in a controlled environment using zebrafish as a model species. | Danio rerio: Random harvested line biological replicate 1 F11 offspring originating from wild caught population | RS1 F11 | breed:missing|dev stage:juvenile|sex:female|tissue:liver|death date:2014|BioSampleModel:Model organism or animal | RNAseq of Danio rerio: juvenile female liver RNA of 4 biological replicates from a random harvested selection line F11 individuals | RS1 F11 | RS1 F11 | Total RNA from liver tissue of juvenile zebrafish was extracted according to the Trizol method with TRI reagent Sigma. The liver tissue was homogenized with a TissueLyser II homogenizer Qiagen. DNase treatment was performed to remove any genomic DNA from the samples. RNA was eluted in RNase free water and RNA concentrations and the integrity of RNA samples were determined using an Agilent 2100 Bioanalyzer. Total RNA was processed by using Low Input Library Preparation Kit to prepare the RNA Seq library. | RNA-Seq | TRANSCRIPTOMIC | RT-PCR | SINGLE | ILLUMINA | Illumina HiSeq 2000 | SRP105243 | RS1-F11B-21_FCC4C2AACXX_L3_WHZEBagiNCAYRAAPEI-202_1.fq.gz RS1-F11B-23_FCC4C2AACXX_L4_WHZEBagiNCAZRAAPEI-203_1.fq.gz RS1-F11B-27_FCC4C2AACXX_L4_WHZEBagiNCBARAAPEI-205_1.fq.gz RS1-F11B-29_FCC4C2AACXX_L4_WHZEBagiNCBBRAAPEI-206_1.fq.gz | fastq fastq fastq fastq | 11947679555.0 | 243830195.0 | RS1 F11B 21 FCC4C2AACXX L3 WHZEBagiNCAYRAAPEI 202 1.fq.gz | 0:49 | A:3159477117;C:2827401119;G:2753495798;T:3206143470;N:1162051 | 49 | 3159477117 | 2827401119 | 2753495798 | 3206143470 | 1162051 | SRX2765557 | SRS2149760 | SRA557107 | University of Turku|Biology | University of Turku | 1 | 0.87877 | 0.04488 | 0.77445 | 0.50852 | 49 | B | usable mapping rate | illumina | hiseq_era | unknown | other | unknown | bulk | unknown | unknown | Finland | 2017-12-04 | Juvenile | Juvenile | Liver | Liver and Biliary System | |||||||||||||||||||||||||||
| 42192 | 42192 | SRR5482142 | SRX2765556 | SRS2149761 | SRP105243 | PRJNA383986 | Danio rerio Raw sequence reads | PRJNA383986 | Other | The goal of the experiment was to simulate the effect of size selective harvesting and captive rearing on gene expression in a controlled environment using zebrafish as a model species. | Danio rerio: Random harvested line biological replicate 2 F11 offspring originating from wild caught population | RS2 F11 | breed:missing|dev stage:juvenile|sex:female|tissue:liver|death date:2014|BioSampleModel:Model organism or animal | RNAseq of Danio rerio: juvenile female liver RNA of 4 biological replicates from a random harvested selection line F11 individuals | RS2 F11 | RS2 F11 | Total RNA from liver tissue of juvenile zebrafish was extracted according to the Trizol method with TRI reagent Sigma. The liver tissue was homogenized with a TissueLyser II homogenizer Qiagen. DNase treatment was performed to remove any genomic DNA from the samples. RNA was eluted in RNase free water and RNA concentrations and the integrity of RNA samples were determined using an Agilent 2100 Bioanalyzer. Total RNA was processed by using Low Input Library Preparation Kit to prepare the RNA Seq library. | RNA-Seq | TRANSCRIPTOMIC | RT-PCR | SINGLE | ILLUMINA | Illumina HiSeq 2000 | SRP105243 | RS2-F11B-34_FCC4CMHACXX_L3_WHZEBagiNCBJRABPEI-214_1.fq.gz RS2-F11B-36_FCC4CMHACXX_L3_WHZEBagiNCBKRABPEI-215_1.fq.gz RS2-F11B-38_FCC4BY1ACXX_L1_WHZEBagiNCBLRABPEI-216_1.fq.gz RS2-F11B-40_FCC4BY1ACXX_L1_WHZEBagiNCBMRABPEI-218_1.fq.gz | fastq fastq fastq fastq | 10619740698.0 | 216729402.0 | RS2 F11B 38 FCC4BY1ACXX L1 WHZEBagiNCBLRABPEI 216 1.fq.gz | 0:49 | A:2799661065;C:2501316593;G:2465115836;T:2852970598;N:676606 | 49 | 2799661065 | 2501316593 | 2465115836 | 2852970598 | 676606 | SRX2765556 | SRS2149761 | SRA557107 | University of Turku|Biology | University of Turku | 1 | 0.8851 | 0.04865 | 0.80407 | 0.54962 | 49 | B | usable mapping rate | illumina | hiseq_era | unknown | other | unknown | bulk | unknown | unknown | Finland | 2017-12-04 | Juvenile | Juvenile | Liver | Liver and Biliary System |
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CREATE TABLE run_metadata("run.accession" VARCHAR, "experiment.accession" VARCHAR, "sample.accession" VARCHAR, "study.accession" VARCHAR, bioproject VARCHAR, "study.title" VARCHAR, "study.alias" VARCHAR, "study.type" VARCHAR, "study.abstract" VARCHAR, "study.attributes" VARCHAR, "study.PMIDs" VARCHAR, "sample.description" VARCHAR, "sample.title" VARCHAR, "sample.alias" VARCHAR, "sample.centername" VARCHAR, "sample.attributes" VARCHAR, "GEOsample.title" VARCHAR, "GEOsample.dataprocessing" VARCHAR, "GEOsample.source" VARCHAR, "GEOsample.treatmentprotocol" VARCHAR, "GEOsample.extractprotocol" VARCHAR, "GEOsample.growthprotocol" VARCHAR, "GEOsample.characteristics" VARCHAR, "GEOsample.accession" VARCHAR, "experiment.title" VARCHAR, "experiment.alias" VARCHAR, "experiment.library_name" VARCHAR, "experiment.design_description" VARCHAR, "experiment.library_construction_protocol" VARCHAR, "experiment.attributes" VARCHAR, "experiment.library_strategy" VARCHAR, "experiment.library_source" VARCHAR, "experiment.library_selection" VARCHAR, "experiment.library_layout" VARCHAR, "experiment.platform" VARCHAR, "experiment.instrument_model" VARCHAR, "experiment.spot_descriptor" VARCHAR, "experiment.study_ref" VARCHAR, "run.title" VARCHAR, "run.attributes" VARCHAR, "run.filename" VARCHAR, "run.semantic_name" VARCHAR, "run.total_bases" DOUBLE, "run.total_spots" DOUBLE, "run.alias" VARCHAR, "run.read_lengths" VARCHAR, "run.base_counts" VARCHAR, "run.r1_length" BIGINT, "run.r2_length" BIGINT, "run.r3_length" BIGINT, "run.r4_length" BIGINT, "run.Acount" BIGINT, "run.Ccount" BIGINT, "run.Gcount" BIGINT, "run.Tcount" BIGINT, "run.Ncount" BIGINT, "run.experiment" VARCHAR, "run.pool_member" VARCHAR, "submission.accession" VARCHAR, "submission.srasource" VARCHAR, "submission.bioprojectsource" VARCHAR, "seqdetective.n_mates" BIGINT, "seqdetective.mapping_rate.mate1" DOUBLE, "seqdetective.mapping_rate.mate2" DOUBLE, "seqdetective.nofeature_rate.mate1" DOUBLE, "seqdetective.nofeature_rate.mate2" DOUBLE, "seqdetective.sparsity.mate1" DOUBLE, "seqdetective.sparsity.mate2" DOUBLE, "seqdetective.pos_strand_rate.mate1" DOUBLE, "seqdetective.pos_strand_rate.mate2" DOUBLE, "seqdetective.readlen.mate1" BIGINT, "seqdetective.readlen.mate2" BIGINT, "seqdetective.judgement.mate1" VARCHAR, "seqdetective.judgement.mate2" VARCHAR, "seqdetective.judgement.reason" VARCHAR, platform_family VARCHAR, instrument_generation VARCHAR, read_bias VARCHAR, selection_class VARCHAR, prep_kit VARCHAR, sc_or_bulk VARCHAR, tech_class VARCHAR, technology VARCHAR, tech_variant VARCHAR, "submission.bioprojectsource.country" VARCHAR, earliest_date DATE, devstage_curation VARCHAR, devstage_curation_coarse VARCHAR, tissue_curation VARCHAR, tissue_curation_coarse VARCHAR);;
CREATE INDEX idx_run_bioproject ON run_metadata(bioproject);;
CREATE INDEX idx_run_run_accession ON run_metadata("run.accession");;