metadata: run_metadata: 42 rows where experiment.library_layout = "SINGLE", experiment.library_selection = "RANDOM" and tissue

42 rows where experiment.library_layout = "SINGLE", experiment.library_selection = "RANDOM" and tissue_curation = "Brain"

descending

Link	rowid ▼	run.accession	experiment.accession	sample.accession	study.accession	bioproject	study.title	study.alias	study.type	study.abstract	study.attributes	sample.description	sample.title	sample.alias	sample.centername	sample.attributes	experiment.title	experiment.alias	experiment.library_name	experiment.design_description	experiment.library_construction_protocol	experiment.attributes	experiment.library_strategy	experiment.library_source	experiment.library_selection	experiment.library_layout	experiment.platform	experiment.instrument_model	experiment.spot_descriptor	experiment.study_ref	run.title	run.attributes	run.filename	run.semantic_name	run.total_bases	run.total_spots	run.alias	run.read_lengths	run.base_counts	run.r1_length	run.Acount	run.Ccount	run.Gcount	run.Tcount	run.Ncount	run.experiment	run.pool_member	submission.accession	submission.srasource	submission.bioprojectsource	seqdetective.n_mates	seqdetective.mapping_rate.mate1	seqdetective.nofeature_rate.mate1	seqdetective.sparsity.mate1	seqdetective.pos_strand_rate.mate1	seqdetective.readlen.mate1	seqdetective.judgement.mate1	seqdetective.judgement.reason	platform_family	instrument_generation	read_bias	selection_class	prep_kit	sc_or_bulk	tech_class	technology	submission.bioprojectsource.country	earliest_date	devstage_curation	devstage_curation_coarse	tissue_curation	tissue_curation_coarse
5795	5795	ERR1698352	ERX1767860	ERS1417534	ERP018188	PRJEB16335	RNA seq of the zebrafish danio rerio brain post developmental exposure to 17a ethinylestradiol	E-MTAB-5173	Transcriptome Analysis	17a ethinylestradiol EE2 is a synthetic estrogen commonly used as an active substance in oral contraceptives. It is frequently found in waste water effluent and raise concern due to its persistent nature. EE2 binds to estrogen receptors with similar affinity to oestradiol and acts as one of the most potent hormone mimics found in the environment. Estrogen is involved in many aspects of the development of the neuroendocrine system influencing both brain structure and behavior. We and others have reported a significant effect on non reproductive behaviors in adult fish and in recent studies we found that developmental exposure to EE2 resulted in an anxiogenic phenotype as adults even post a long remediation period. In this study we aim to study possible mechanisms behind the behavior alterations of zebrafish developmentally exposed to EE2 by sequencing the whole brain transcriptome. Zebrafish embryos were exposed to 0 2.14 and 7.34 ng/L EE2 from 1 day to 80 dpf post the exposure period a remediation period of 120 days followed before the fish were sampled. 3 male brains from the control group 0 ng/L and the 2.14 ng/L group were sampled and 3 female brains from the control group 0 ng/L and 7.34 ng/L were sampled.	ENA FIRST PUBLIC:2017 10 02\|ENA LAST UPDATE:2016 10 19\|ArrayExpress:E MTAB 5173	Protocols: RNA extraction Whole brains homogenized in TriReagent according to the manufacturer 0.8 ml/sample Sigma Aldrich Germany EE2 exposure. Fish exposed to 0 2.14 or 7.34 ng/L EE2 for 80 days starting 1 dpf Ion Whole transcriptome analysis kit on RiboZero eukaryote depleted total RNA	IonXpressRNA 012 up 058 12	SAMEA4518355	School of Natural Science, Technology and Environmental Studies Sodertorn University	ENA first public:2017 10 02\|ENA last update:2016 10 19\|External Id:SAMEA4518355\|INSDC center alias:School of Natural Science Technology and Environmental Studies Sodertorn University\|INSDC center name:School of Natural Science Technology and Environmental Studies Sodertorn University\|INSDC first public:2017 10 02T17:05:16Z\|INSDC last update:2016 10 19T13:53:59Z\|INSDC status:public\|Submitter Id:E MTAB 5173:IonXpressRNA 012 up 058 12\|age:200\|broker name:ArrayExpress\|common name:zebrafish\|organism part:brain\|sample name:E MTAB 5173:IonXpressRNA 012 up 058 12\|sex:male	Ion Torrent Proton sequencing; RNA seq of the zebrafish danio rerio brain post developmental exposure to 17α ethinylestradiol	E MTAB 5173:IonXpressRNA 012 up 058 12 s	IonXpressRNA 012 up 058 12 s	RNA seq of the zebrafish danio rerio brain post developmental exposure to 17α ethinylestradiol	RNA extraction Whole brains homogenized in TriReagent according to the manufacturer 0.8 ml/sample Sigma Aldrich Germany EE2 exposure. Fish exposed to 0 2.14 or 7.34 ng/L EE2 for 80 days starting 1 dpf Ion Whole transcriptome analysis kit on RiboZero eukaryote depleted total RNA	Experimental Factor: male:sex\|Experimental Factor: 17 ethinylestradiol:compound\|Experimental Factor: 2.14:dose	RNA-Seq	TRANSCRIPTOMIC	RANDOM	SINGLE	ION_TORRENT	Ion Torrent Proton		ERP018188	Ion Torrent Proton sequencing; RNA seq of the zebrafish danio rerio brain post developmental exposure to 17Î± ethinylestradiol	ENA FIRST PUBLIC:2017 10 02\|ENA LAST UPDATE:2018 11 16	IonXpressRNA_012_up_058_12.fastq.gz	fastq	2535299006.0	33956077.0	E MTAB 5173:IonXpressRNA 012 up 058 12	0:74.66	A:719061985;C:581906269;G:579932234;T:654398518;N:0	74	719061985	581906269	579932234	654398518	0	ERX1767860	ERS1417534	ERA739176	School of Natural Science, Technology and Environmental Studies Sodertorn University\|European Nucleotide Archive	School of Natural Science, Technology and Environmental Studies Sodertorn University\|European Nucleotide Archive	1	0.7343	0.34119	0.7359	0.48406	66	B	usable mapping rate	ion_torrent	ion_torrent	full_length	random_priming	ribozero	bulk	unknown	unknown	Sweden	2016-10-19	Pharyngula	Embryo	Brain	Nervous System
5796	5796	ERR1698351	ERX1767859	ERS1417533	ERP018188	PRJEB16335	RNA seq of the zebrafish danio rerio brain post developmental exposure to 17a ethinylestradiol	E-MTAB-5173	Transcriptome Analysis	17a ethinylestradiol EE2 is a synthetic estrogen commonly used as an active substance in oral contraceptives. It is frequently found in waste water effluent and raise concern due to its persistent nature. EE2 binds to estrogen receptors with similar affinity to oestradiol and acts as one of the most potent hormone mimics found in the environment. Estrogen is involved in many aspects of the development of the neuroendocrine system influencing both brain structure and behavior. We and others have reported a significant effect on non reproductive behaviors in adult fish and in recent studies we found that developmental exposure to EE2 resulted in an anxiogenic phenotype as adults even post a long remediation period. In this study we aim to study possible mechanisms behind the behavior alterations of zebrafish developmentally exposed to EE2 by sequencing the whole brain transcriptome. Zebrafish embryos were exposed to 0 2.14 and 7.34 ng/L EE2 from 1 day to 80 dpf post the exposure period a remediation period of 120 days followed before the fish were sampled. 3 male brains from the control group 0 ng/L and the 2.14 ng/L group were sampled and 3 female brains from the control group 0 ng/L and 7.34 ng/L were sampled.	ENA FIRST PUBLIC:2017 10 02\|ENA LAST UPDATE:2016 10 19\|ArrayExpress:E MTAB 5173	Protocols: RNA extraction Whole brains homogenized in TriReagent according to the manufacturer 0.8 ml/sample Sigma Aldrich Germany EE2 exposure. Fish exposed to 0 2.14 or 7.34 ng/L EE2 for 80 days starting 1 dpf Ion Whole transcriptome analysis kit on RiboZero eukaryote depleted total RNA	IonXpressRNA 011 up 058 11	SAMEA4518354	School of Natural Science, Technology and Environmental Studies Sodertorn University	ENA first public:2017 10 02\|ENA last update:2016 10 19\|External Id:SAMEA4518354\|INSDC center alias:School of Natural Science Technology and Environmental Studies Sodertorn University\|INSDC center name:School of Natural Science Technology and Environmental Studies Sodertorn University\|INSDC first public:2017 10 02T17:05:16Z\|INSDC last update:2016 10 19T13:53:59Z\|INSDC status:public\|Submitter Id:E MTAB 5173:IonXpressRNA 011 up 058 11\|age:200\|broker name:ArrayExpress\|common name:zebrafish\|organism part:brain\|sample name:E MTAB 5173:IonXpressRNA 011 up 058 11\|sex:male	Ion Torrent Proton sequencing; RNA seq of the zebrafish danio rerio brain post developmental exposure to 17α ethinylestradiol	E MTAB 5173:IonXpressRNA 011 up 058 11 s	IonXpressRNA 011 up 058 11 s	RNA seq of the zebrafish danio rerio brain post developmental exposure to 17α ethinylestradiol	RNA extraction Whole brains homogenized in TriReagent according to the manufacturer 0.8 ml/sample Sigma Aldrich Germany EE2 exposure. Fish exposed to 0 2.14 or 7.34 ng/L EE2 for 80 days starting 1 dpf Ion Whole transcriptome analysis kit on RiboZero eukaryote depleted total RNA	Experimental Factor: male:sex\|Experimental Factor: 17 ethinylestradiol:compound\|Experimental Factor: 2.14:dose	RNA-Seq	TRANSCRIPTOMIC	RANDOM	SINGLE	ION_TORRENT	Ion Torrent Proton		ERP018188	Ion Torrent Proton sequencing; RNA seq of the zebrafish danio rerio brain post developmental exposure to 17Î± ethinylestradiol	ENA FIRST PUBLIC:2017 10 02\|ENA LAST UPDATE:2018 11 16	IonXpressRNA_011_up_058_11.fastq.gz	fastq	3400774471.0	38709756.0	E MTAB 5173:IonXpressRNA 011 up 058 11	0:87.85	A:948991439;C:793389977;G:792539547;T:865853508;N:0	87	948991439	793389977	792539547	865853508	0	ERX1767859	ERS1417533	ERA739176	School of Natural Science, Technology and Environmental Studies Sodertorn University\|European Nucleotide Archive	School of Natural Science, Technology and Environmental Studies Sodertorn University\|European Nucleotide Archive	1	0.63968	0.27033	0.75213	0.47966	139	B	usable mapping rate	ion_torrent	ion_torrent	full_length	random_priming	ribozero	bulk	unknown	unknown	Sweden	2016-10-19	Pharyngula	Embryo	Brain	Nervous System
5797	5797	ERR1698350	ERX1767858	ERS1417532	ERP018188	PRJEB16335	RNA seq of the zebrafish danio rerio brain post developmental exposure to 17a ethinylestradiol	E-MTAB-5173	Transcriptome Analysis	17a ethinylestradiol EE2 is a synthetic estrogen commonly used as an active substance in oral contraceptives. It is frequently found in waste water effluent and raise concern due to its persistent nature. EE2 binds to estrogen receptors with similar affinity to oestradiol and acts as one of the most potent hormone mimics found in the environment. Estrogen is involved in many aspects of the development of the neuroendocrine system influencing both brain structure and behavior. We and others have reported a significant effect on non reproductive behaviors in adult fish and in recent studies we found that developmental exposure to EE2 resulted in an anxiogenic phenotype as adults even post a long remediation period. In this study we aim to study possible mechanisms behind the behavior alterations of zebrafish developmentally exposed to EE2 by sequencing the whole brain transcriptome. Zebrafish embryos were exposed to 0 2.14 and 7.34 ng/L EE2 from 1 day to 80 dpf post the exposure period a remediation period of 120 days followed before the fish were sampled. 3 male brains from the control group 0 ng/L and the 2.14 ng/L group were sampled and 3 female brains from the control group 0 ng/L and 7.34 ng/L were sampled.	ENA FIRST PUBLIC:2017 10 02\|ENA LAST UPDATE:2016 10 19\|ArrayExpress:E MTAB 5173	Protocols: RNA extraction Whole brains homogenized in TriReagent according to the manufacturer 0.8 ml/sample Sigma Aldrich Germany EE2 exposure. Fish exposed to 0 2.14 or 7.34 ng/L EE2 for 80 days starting 1 dpf Ion Whole transcriptome analysis kit on RiboZero eukaryote depleted total RNA	IonXpressRNA 010 up 058 10	SAMEA4518353	School of Natural Science, Technology and Environmental Studies Sodertorn University	ENA first public:2017 10 02\|ENA last update:2016 10 19\|External Id:SAMEA4518353\|INSDC center alias:School of Natural Science Technology and Environmental Studies Sodertorn University\|INSDC center name:School of Natural Science Technology and Environmental Studies Sodertorn University\|INSDC first public:2017 10 02T17:05:16Z\|INSDC last update:2016 10 19T13:53:59Z\|INSDC status:public\|Submitter Id:E MTAB 5173:IonXpressRNA 010 up 058 10\|age:200\|broker name:ArrayExpress\|common name:zebrafish\|organism part:brain\|sample name:E MTAB 5173:IonXpressRNA 010 up 058 10\|sex:male	Ion Torrent Proton sequencing; RNA seq of the zebrafish danio rerio brain post developmental exposure to 17α ethinylestradiol	E MTAB 5173:IonXpressRNA 010 up 058 10 s	IonXpressRNA 010 up 058 10 s	RNA seq of the zebrafish danio rerio brain post developmental exposure to 17α ethinylestradiol	RNA extraction Whole brains homogenized in TriReagent according to the manufacturer 0.8 ml/sample Sigma Aldrich Germany EE2 exposure. Fish exposed to 0 2.14 or 7.34 ng/L EE2 for 80 days starting 1 dpf Ion Whole transcriptome analysis kit on RiboZero eukaryote depleted total RNA	Experimental Factor: male:sex\|Experimental Factor: 17 ethinylestradiol:compound\|Experimental Factor: 2.14:dose	RNA-Seq	TRANSCRIPTOMIC	RANDOM	SINGLE	ION_TORRENT	Ion Torrent Proton		ERP018188	Ion Torrent Proton sequencing; RNA seq of the zebrafish danio rerio brain post developmental exposure to 17Î± ethinylestradiol	ENA FIRST PUBLIC:2017 10 02\|ENA LAST UPDATE:2018 11 16	IonXpressRNA_010_up_058_10.fastq.gz	fastq	3502667998.0	39204781.0	E MTAB 5173:IonXpressRNA 010 up 058 10	0:89.34	A:976977303;C:803330255;G:808731995;T:913628445;N:0	89	976977303	803330255	808731995	913628445	0	ERX1767858	ERS1417532	ERA739176	School of Natural Science, Technology and Environmental Studies Sodertorn University\|European Nucleotide Archive	School of Natural Science, Technology and Environmental Studies Sodertorn University\|European Nucleotide Archive	1	0.65846	0.2815	0.74028	0.47784	86	B	usable mapping rate	ion_torrent	ion_torrent	full_length	random_priming	ribozero	bulk	unknown	unknown	Sweden	2016-10-19	Pharyngula	Embryo	Brain	Nervous System
5798	5798	ERR1698349	ERX1767857	ERS1417531	ERP018188	PRJEB16335	RNA seq of the zebrafish danio rerio brain post developmental exposure to 17a ethinylestradiol	E-MTAB-5173	Transcriptome Analysis	17a ethinylestradiol EE2 is a synthetic estrogen commonly used as an active substance in oral contraceptives. It is frequently found in waste water effluent and raise concern due to its persistent nature. EE2 binds to estrogen receptors with similar affinity to oestradiol and acts as one of the most potent hormone mimics found in the environment. Estrogen is involved in many aspects of the development of the neuroendocrine system influencing both brain structure and behavior. We and others have reported a significant effect on non reproductive behaviors in adult fish and in recent studies we found that developmental exposure to EE2 resulted in an anxiogenic phenotype as adults even post a long remediation period. In this study we aim to study possible mechanisms behind the behavior alterations of zebrafish developmentally exposed to EE2 by sequencing the whole brain transcriptome. Zebrafish embryos were exposed to 0 2.14 and 7.34 ng/L EE2 from 1 day to 80 dpf post the exposure period a remediation period of 120 days followed before the fish were sampled. 3 male brains from the control group 0 ng/L and the 2.14 ng/L group were sampled and 3 female brains from the control group 0 ng/L and 7.34 ng/L were sampled.	ENA FIRST PUBLIC:2017 10 02\|ENA LAST UPDATE:2016 10 19\|ArrayExpress:E MTAB 5173	Protocols: RNA extraction Whole brains homogenized in TriReagent according to the manufacturer 0.8 ml/sample Sigma Aldrich Germany EE2 exposure. Fish exposed to 0 2.14 or 7.34 ng/L EE2 for 80 days starting 1 dpf Ion Whole transcriptome analysis kit on RiboZero eukaryote depleted total RNA	IonXpressRNA 009 up 058 9	SAMEA4518352	School of Natural Science, Technology and Environmental Studies Sodertorn University	ENA first public:2017 10 02\|ENA last update:2016 10 19\|External Id:SAMEA4518352\|INSDC center alias:School of Natural Science Technology and Environmental Studies Sodertorn University\|INSDC center name:School of Natural Science Technology and Environmental Studies Sodertorn University\|INSDC first public:2017 10 02T17:05:16Z\|INSDC last update:2016 10 19T13:53:59Z\|INSDC status:public\|Submitter Id:E MTAB 5173:IonXpressRNA 009 up 058 9\|age:200\|broker name:ArrayExpress\|common name:zebrafish\|organism part:brain\|sample name:E MTAB 5173:IonXpressRNA 009 up 058 9\|sex:male	Ion Torrent Proton sequencing; RNA seq of the zebrafish danio rerio brain post developmental exposure to 17α ethinylestradiol	E MTAB 5173:IonXpressRNA 009 up 058 9 s	IonXpressRNA 009 up 058 9 s	RNA seq of the zebrafish danio rerio brain post developmental exposure to 17α ethinylestradiol	RNA extraction Whole brains homogenized in TriReagent according to the manufacturer 0.8 ml/sample Sigma Aldrich Germany EE2 exposure. Fish exposed to 0 2.14 or 7.34 ng/L EE2 for 80 days starting 1 dpf Ion Whole transcriptome analysis kit on RiboZero eukaryote depleted total RNA	Experimental Factor: male:sex\|Experimental Factor: n1:compound	RNA-Seq	TRANSCRIPTOMIC	RANDOM	SINGLE	ION_TORRENT	Ion Torrent Proton		ERP018188	Ion Torrent Proton sequencing; RNA seq of the zebrafish danio rerio brain post developmental exposure to 17Î± ethinylestradiol	ENA FIRST PUBLIC:2017 10 02\|ENA LAST UPDATE:2018 11 16	IonXpressRNA_009_up_058_9.fastq.gz	fastq	3575088264.0	39745481.0	E MTAB 5173:IonXpressRNA 009 up 058 9	0:89.95	A:1011474126;C:809896078;G:807471974;T:946246086;N:0	89	1011474126	809896078	807471974	946246086	0	ERX1767857	ERS1417531	ERA739176	School of Natural Science, Technology and Environmental Studies Sodertorn University\|European Nucleotide Archive	School of Natural Science, Technology and Environmental Studies Sodertorn University\|European Nucleotide Archive	1	0.65266	0.30274	0.75286	0.48527	94	B	usable mapping rate	ion_torrent	ion_torrent	full_length	random_priming	ribozero	bulk	unknown	unknown	Sweden	2016-10-19	Pharyngula	Embryo	Brain	Nervous System
5799	5799	ERR1698348	ERX1767856	ERS1417530	ERP018188	PRJEB16335	RNA seq of the zebrafish danio rerio brain post developmental exposure to 17a ethinylestradiol	E-MTAB-5173	Transcriptome Analysis	17a ethinylestradiol EE2 is a synthetic estrogen commonly used as an active substance in oral contraceptives. It is frequently found in waste water effluent and raise concern due to its persistent nature. EE2 binds to estrogen receptors with similar affinity to oestradiol and acts as one of the most potent hormone mimics found in the environment. Estrogen is involved in many aspects of the development of the neuroendocrine system influencing both brain structure and behavior. We and others have reported a significant effect on non reproductive behaviors in adult fish and in recent studies we found that developmental exposure to EE2 resulted in an anxiogenic phenotype as adults even post a long remediation period. In this study we aim to study possible mechanisms behind the behavior alterations of zebrafish developmentally exposed to EE2 by sequencing the whole brain transcriptome. Zebrafish embryos were exposed to 0 2.14 and 7.34 ng/L EE2 from 1 day to 80 dpf post the exposure period a remediation period of 120 days followed before the fish were sampled. 3 male brains from the control group 0 ng/L and the 2.14 ng/L group were sampled and 3 female brains from the control group 0 ng/L and 7.34 ng/L were sampled.	ENA FIRST PUBLIC:2017 10 02\|ENA LAST UPDATE:2016 10 19\|ArrayExpress:E MTAB 5173	Protocols: RNA extraction Whole brains homogenized in TriReagent according to the manufacturer 0.8 ml/sample Sigma Aldrich Germany EE2 exposure. Fish exposed to 0 2.14 or 7.34 ng/L EE2 for 80 days starting 1 dpf Ion Whole transcriptome analysis kit on RiboZero eukaryote depleted total RNA	IonXpressRNA 008 up 058 8	SAMEA4518351	School of Natural Science, Technology and Environmental Studies Sodertorn University	ENA first public:2017 10 02\|ENA last update:2016 10 19\|External Id:SAMEA4518351\|INSDC center alias:School of Natural Science Technology and Environmental Studies Sodertorn University\|INSDC center name:School of Natural Science Technology and Environmental Studies Sodertorn University\|INSDC first public:2017 10 02T17:05:16Z\|INSDC last update:2016 10 19T13:53:59Z\|INSDC status:public\|Submitter Id:E MTAB 5173:IonXpressRNA 008 up 058 8\|age:200\|broker name:ArrayExpress\|common name:zebrafish\|organism part:brain\|sample name:E MTAB 5173:IonXpressRNA 008 up 058 8\|sex:male	Ion Torrent Proton sequencing; RNA seq of the zebrafish danio rerio brain post developmental exposure to 17α ethinylestradiol	E MTAB 5173:IonXpressRNA 008 up 058 8 s	IonXpressRNA 008 up 058 8 s	RNA seq of the zebrafish danio rerio brain post developmental exposure to 17α ethinylestradiol	RNA extraction Whole brains homogenized in TriReagent according to the manufacturer 0.8 ml/sample Sigma Aldrich Germany EE2 exposure. Fish exposed to 0 2.14 or 7.34 ng/L EE2 for 80 days starting 1 dpf Ion Whole transcriptome analysis kit on RiboZero eukaryote depleted total RNA	Experimental Factor: male:sex\|Experimental Factor: n1:compound	RNA-Seq	TRANSCRIPTOMIC	RANDOM	SINGLE	ION_TORRENT	Ion Torrent Proton		ERP018188	Ion Torrent Proton sequencing; RNA seq of the zebrafish danio rerio brain post developmental exposure to 17Î± ethinylestradiol	ENA FIRST PUBLIC:2017 10 02\|ENA LAST UPDATE:2018 11 16	IonXpressRNA_008_up_058_8.fastq.gz	fastq	3259710114.0	38530802.0	E MTAB 5173:IonXpressRNA 008 up 058 8	0:84.60	A:916053004;C:755694888;G:751125332;T:836836890;N:0	84	916053004	755694888	751125332	836836890	0	ERX1767856	ERS1417530	ERA739176	School of Natural Science, Technology and Environmental Studies Sodertorn University\|European Nucleotide Archive	School of Natural Science, Technology and Environmental Studies Sodertorn University\|European Nucleotide Archive	1	0.70995	0.31365	0.74781	0.48846	61	B	usable mapping rate	ion_torrent	ion_torrent	full_length	random_priming	ribozero	bulk	unknown	unknown	Sweden	2016-10-19	Pharyngula	Embryo	Brain	Nervous System
5800	5800	ERR1698347	ERX1767855	ERS1417529	ERP018188	PRJEB16335	RNA seq of the zebrafish danio rerio brain post developmental exposure to 17a ethinylestradiol	E-MTAB-5173	Transcriptome Analysis	17a ethinylestradiol EE2 is a synthetic estrogen commonly used as an active substance in oral contraceptives. It is frequently found in waste water effluent and raise concern due to its persistent nature. EE2 binds to estrogen receptors with similar affinity to oestradiol and acts as one of the most potent hormone mimics found in the environment. Estrogen is involved in many aspects of the development of the neuroendocrine system influencing both brain structure and behavior. We and others have reported a significant effect on non reproductive behaviors in adult fish and in recent studies we found that developmental exposure to EE2 resulted in an anxiogenic phenotype as adults even post a long remediation period. In this study we aim to study possible mechanisms behind the behavior alterations of zebrafish developmentally exposed to EE2 by sequencing the whole brain transcriptome. Zebrafish embryos were exposed to 0 2.14 and 7.34 ng/L EE2 from 1 day to 80 dpf post the exposure period a remediation period of 120 days followed before the fish were sampled. 3 male brains from the control group 0 ng/L and the 2.14 ng/L group were sampled and 3 female brains from the control group 0 ng/L and 7.34 ng/L were sampled.	ENA FIRST PUBLIC:2017 10 02\|ENA LAST UPDATE:2016 10 19\|ArrayExpress:E MTAB 5173	Protocols: RNA extraction Whole brains homogenized in TriReagent according to the manufacturer 0.8 ml/sample Sigma Aldrich Germany EE2 exposure. Fish exposed to 0 2.14 or 7.34 ng/L EE2 for 80 days starting 1 dpf Ion Whole transcriptome analysis kit on RiboZero eukaryote depleted total RNA	IonXpressRNA 007 up 058 7	SAMEA4518350	School of Natural Science, Technology and Environmental Studies Sodertorn University	ENA first public:2017 10 02\|ENA last update:2016 10 19\|External Id:SAMEA4518350\|INSDC center alias:School of Natural Science Technology and Environmental Studies Sodertorn University\|INSDC center name:School of Natural Science Technology and Environmental Studies Sodertorn University\|INSDC first public:2017 10 02T17:05:16Z\|INSDC last update:2016 10 19T13:53:59Z\|INSDC status:public\|Submitter Id:E MTAB 5173:IonXpressRNA 007 up 058 7\|age:200\|broker name:ArrayExpress\|common name:zebrafish\|organism part:brain\|sample name:E MTAB 5173:IonXpressRNA 007 up 058 7\|sex:male	Ion Torrent Proton sequencing; RNA seq of the zebrafish danio rerio brain post developmental exposure to 17α ethinylestradiol	E MTAB 5173:IonXpressRNA 007 up 058 7 s	IonXpressRNA 007 up 058 7 s	RNA seq of the zebrafish danio rerio brain post developmental exposure to 17α ethinylestradiol	RNA extraction Whole brains homogenized in TriReagent according to the manufacturer 0.8 ml/sample Sigma Aldrich Germany EE2 exposure. Fish exposed to 0 2.14 or 7.34 ng/L EE2 for 80 days starting 1 dpf Ion Whole transcriptome analysis kit on RiboZero eukaryote depleted total RNA	Experimental Factor: male:sex\|Experimental Factor: n1:compound	RNA-Seq	TRANSCRIPTOMIC	RANDOM	SINGLE	ION_TORRENT	Ion Torrent Proton		ERP018188	Ion Torrent Proton sequencing; RNA seq of the zebrafish danio rerio brain post developmental exposure to 17Î± ethinylestradiol	ENA FIRST PUBLIC:2017 10 02\|ENA LAST UPDATE:2018 11 16	IonXpressRNA_007_up_058_7.fastq.gz	fastq	2307842164.0	28984666.0	E MTAB 5173:IonXpressRNA 007 up 058 7	0:79.62	A:634041732;C:548565089;G:545418985;T:579816358;N:0	79	634041732	548565089	545418985	579816358	0	ERX1767855	ERS1417529	ERA739176	School of Natural Science, Technology and Environmental Studies Sodertorn University\|European Nucleotide Archive	School of Natural Science, Technology and Environmental Studies Sodertorn University\|European Nucleotide Archive	1	0.67542	0.2817	0.74168	0.47224	37	B	usable mapping rate	ion_torrent	ion_torrent	full_length	random_priming	ribozero	bulk	unknown	unknown	Sweden	2016-10-19	Pharyngula	Embryo	Brain	Nervous System
5801	5801	ERR1698346	ERX1767854	ERS1417528	ERP018188	PRJEB16335	RNA seq of the zebrafish danio rerio brain post developmental exposure to 17a ethinylestradiol	E-MTAB-5173	Transcriptome Analysis	17a ethinylestradiol EE2 is a synthetic estrogen commonly used as an active substance in oral contraceptives. It is frequently found in waste water effluent and raise concern due to its persistent nature. EE2 binds to estrogen receptors with similar affinity to oestradiol and acts as one of the most potent hormone mimics found in the environment. Estrogen is involved in many aspects of the development of the neuroendocrine system influencing both brain structure and behavior. We and others have reported a significant effect on non reproductive behaviors in adult fish and in recent studies we found that developmental exposure to EE2 resulted in an anxiogenic phenotype as adults even post a long remediation period. In this study we aim to study possible mechanisms behind the behavior alterations of zebrafish developmentally exposed to EE2 by sequencing the whole brain transcriptome. Zebrafish embryos were exposed to 0 2.14 and 7.34 ng/L EE2 from 1 day to 80 dpf post the exposure period a remediation period of 120 days followed before the fish were sampled. 3 male brains from the control group 0 ng/L and the 2.14 ng/L group were sampled and 3 female brains from the control group 0 ng/L and 7.34 ng/L were sampled.	ENA FIRST PUBLIC:2017 10 02\|ENA LAST UPDATE:2016 10 19\|ArrayExpress:E MTAB 5173	Protocols: RNA extraction Whole brains homogenized in TriReagent according to the manufacturer 0.8 ml/sample Sigma Aldrich Germany EE2 exposure. Fish exposed to 0 2.14 or 7.34 ng/L EE2 for 80 days starting 1 dpf Ion Whole transcriptome analysis kit on RiboZero eukaryote depleted total RNA	IonXpressRNA 006 up 058 6	SAMEA4518349	School of Natural Science, Technology and Environmental Studies Sodertorn University	ENA first public:2017 10 02\|ENA last update:2016 10 19\|External Id:SAMEA4518349\|INSDC center alias:School of Natural Science Technology and Environmental Studies Sodertorn University\|INSDC center name:School of Natural Science Technology and Environmental Studies Sodertorn University\|INSDC first public:2017 10 02T17:05:16Z\|INSDC last update:2016 10 19T13:53:59Z\|INSDC status:public\|Submitter Id:E MTAB 5173:IonXpressRNA 006 up 058 6\|age:200\|broker name:ArrayExpress\|common name:zebrafish\|organism part:brain\|sample name:E MTAB 5173:IonXpressRNA 006 up 058 6\|sex:female	Ion Torrent Proton sequencing; RNA seq of the zebrafish danio rerio brain post developmental exposure to 17α ethinylestradiol	E MTAB 5173:IonXpressRNA 006 up 058 6 s	IonXpressRNA 006 up 058 6 s	RNA seq of the zebrafish danio rerio brain post developmental exposure to 17α ethinylestradiol	RNA extraction Whole brains homogenized in TriReagent according to the manufacturer 0.8 ml/sample Sigma Aldrich Germany EE2 exposure. Fish exposed to 0 2.14 or 7.34 ng/L EE2 for 80 days starting 1 dpf Ion Whole transcriptome analysis kit on RiboZero eukaryote depleted total RNA	Experimental Factor: female:sex\|Experimental Factor: 17 ethinylestradiol:compound\|Experimental Factor: 7.34:dose	RNA-Seq	TRANSCRIPTOMIC	RANDOM	SINGLE	ION_TORRENT	Ion Torrent Proton		ERP018188	Ion Torrent Proton sequencing; RNA seq of the zebrafish danio rerio brain post developmental exposure to 17Î± ethinylestradiol	ENA FIRST PUBLIC:2017 10 02\|ENA LAST UPDATE:2018 11 16	IonXpressRNA_006_up_058_6.fastq.gz	fastq	2239484857.0	27021234.0	E MTAB 5173:IonXpressRNA 006 up 058 6	0:82.88	A:637326624;C:506830056;G:503508207;T:591819970;N:0	82	637326624	506830056	503508207	591819970	0	ERX1767854	ERS1417528	ERA739176	School of Natural Science, Technology and Environmental Studies Sodertorn University\|European Nucleotide Archive	School of Natural Science, Technology and Environmental Studies Sodertorn University\|European Nucleotide Archive	1	0.66441	0.31904	0.74905	0.47154	122	B	usable mapping rate	ion_torrent	ion_torrent	full_length	random_priming	ribozero	bulk	unknown	unknown	Sweden	2016-10-19	Pharyngula	Embryo	Brain	Nervous System
5802	5802	ERR1698345	ERX1767853	ERS1417527	ERP018188	PRJEB16335	RNA seq of the zebrafish danio rerio brain post developmental exposure to 17a ethinylestradiol	E-MTAB-5173	Transcriptome Analysis	17a ethinylestradiol EE2 is a synthetic estrogen commonly used as an active substance in oral contraceptives. It is frequently found in waste water effluent and raise concern due to its persistent nature. EE2 binds to estrogen receptors with similar affinity to oestradiol and acts as one of the most potent hormone mimics found in the environment. Estrogen is involved in many aspects of the development of the neuroendocrine system influencing both brain structure and behavior. We and others have reported a significant effect on non reproductive behaviors in adult fish and in recent studies we found that developmental exposure to EE2 resulted in an anxiogenic phenotype as adults even post a long remediation period. In this study we aim to study possible mechanisms behind the behavior alterations of zebrafish developmentally exposed to EE2 by sequencing the whole brain transcriptome. Zebrafish embryos were exposed to 0 2.14 and 7.34 ng/L EE2 from 1 day to 80 dpf post the exposure period a remediation period of 120 days followed before the fish were sampled. 3 male brains from the control group 0 ng/L and the 2.14 ng/L group were sampled and 3 female brains from the control group 0 ng/L and 7.34 ng/L were sampled.	ENA FIRST PUBLIC:2017 10 02\|ENA LAST UPDATE:2016 10 19\|ArrayExpress:E MTAB 5173	Protocols: RNA extraction Whole brains homogenized in TriReagent according to the manufacturer 0.8 ml/sample Sigma Aldrich Germany EE2 exposure. Fish exposed to 0 2.14 or 7.34 ng/L EE2 for 80 days starting 1 dpf Ion Whole transcriptome analysis kit on RiboZero eukaryote depleted total RNA	IonXpressRNA 005 up 058 5	SAMEA4518348	School of Natural Science, Technology and Environmental Studies Sodertorn University	ENA first public:2017 10 02\|ENA last update:2016 10 19\|External Id:SAMEA4518348\|INSDC center alias:School of Natural Science Technology and Environmental Studies Sodertorn University\|INSDC center name:School of Natural Science Technology and Environmental Studies Sodertorn University\|INSDC first public:2017 10 02T17:05:16Z\|INSDC last update:2016 10 19T13:53:59Z\|INSDC status:public\|Submitter Id:E MTAB 5173:IonXpressRNA 005 up 058 5\|age:200\|broker name:ArrayExpress\|common name:zebrafish\|organism part:brain\|sample name:E MTAB 5173:IonXpressRNA 005 up 058 5\|sex:female	Ion Torrent Proton sequencing; RNA seq of the zebrafish danio rerio brain post developmental exposure to 17α ethinylestradiol	E MTAB 5173:IonXpressRNA 005 up 058 5 s	IonXpressRNA 005 up 058 5 s	RNA seq of the zebrafish danio rerio brain post developmental exposure to 17α ethinylestradiol	RNA extraction Whole brains homogenized in TriReagent according to the manufacturer 0.8 ml/sample Sigma Aldrich Germany EE2 exposure. Fish exposed to 0 2.14 or 7.34 ng/L EE2 for 80 days starting 1 dpf Ion Whole transcriptome analysis kit on RiboZero eukaryote depleted total RNA	Experimental Factor: female:sex\|Experimental Factor: 17 ethinylestradiol:compound\|Experimental Factor: 7.34:dose	RNA-Seq	TRANSCRIPTOMIC	RANDOM	SINGLE	ION_TORRENT	Ion Torrent Proton		ERP018188	Ion Torrent Proton sequencing; RNA seq of the zebrafish danio rerio brain post developmental exposure to 17Î± ethinylestradiol	ENA FIRST PUBLIC:2017 10 02\|ENA LAST UPDATE:2018 11 16	IonXpressRNA_005_up_058_5.fastq.gz	fastq	2920608487.0	39573075.0	E MTAB 5173:IonXpressRNA 005 up 058 5	0:73.80	A:814851327;C:678116994;G:691911192;T:735728974;N:0	73	814851327	678116994	691911192	735728974	0	ERX1767853	ERS1417527	ERA739176	School of Natural Science, Technology and Environmental Studies Sodertorn University\|European Nucleotide Archive	School of Natural Science, Technology and Environmental Studies Sodertorn University\|European Nucleotide Archive	1	0.60005	0.25542	0.76481	0.4886	12	B	usable mapping rate	ion_torrent	ion_torrent	full_length	random_priming	ribozero	bulk	unknown	unknown	Sweden	2016-10-19	Pharyngula	Embryo	Brain	Nervous System
5803	5803	ERR1698344	ERX1767852	ERS1417526	ERP018188	PRJEB16335	RNA seq of the zebrafish danio rerio brain post developmental exposure to 17a ethinylestradiol	E-MTAB-5173	Transcriptome Analysis	17a ethinylestradiol EE2 is a synthetic estrogen commonly used as an active substance in oral contraceptives. It is frequently found in waste water effluent and raise concern due to its persistent nature. EE2 binds to estrogen receptors with similar affinity to oestradiol and acts as one of the most potent hormone mimics found in the environment. Estrogen is involved in many aspects of the development of the neuroendocrine system influencing both brain structure and behavior. We and others have reported a significant effect on non reproductive behaviors in adult fish and in recent studies we found that developmental exposure to EE2 resulted in an anxiogenic phenotype as adults even post a long remediation period. In this study we aim to study possible mechanisms behind the behavior alterations of zebrafish developmentally exposed to EE2 by sequencing the whole brain transcriptome. Zebrafish embryos were exposed to 0 2.14 and 7.34 ng/L EE2 from 1 day to 80 dpf post the exposure period a remediation period of 120 days followed before the fish were sampled. 3 male brains from the control group 0 ng/L and the 2.14 ng/L group were sampled and 3 female brains from the control group 0 ng/L and 7.34 ng/L were sampled.	ENA FIRST PUBLIC:2017 10 02\|ENA LAST UPDATE:2016 10 19\|ArrayExpress:E MTAB 5173	Protocols: RNA extraction Whole brains homogenized in TriReagent according to the manufacturer 0.8 ml/sample Sigma Aldrich Germany EE2 exposure. Fish exposed to 0 2.14 or 7.34 ng/L EE2 for 80 days starting 1 dpf Ion Whole transcriptome analysis kit on RiboZero eukaryote depleted total RNA	IonXpressRNA 004 up 058 4	SAMEA4518347	School of Natural Science, Technology and Environmental Studies Sodertorn University	ENA first public:2017 10 02\|ENA last update:2016 10 19\|External Id:SAMEA4518347\|INSDC center alias:School of Natural Science Technology and Environmental Studies Sodertorn University\|INSDC center name:School of Natural Science Technology and Environmental Studies Sodertorn University\|INSDC first public:2017 10 02T17:05:16Z\|INSDC last update:2016 10 19T13:53:59Z\|INSDC status:public\|Submitter Id:E MTAB 5173:IonXpressRNA 004 up 058 4\|age:200\|broker name:ArrayExpress\|common name:zebrafish\|organism part:brain\|sample name:E MTAB 5173:IonXpressRNA 004 up 058 4\|sex:female	Ion Torrent Proton sequencing; RNA seq of the zebrafish danio rerio brain post developmental exposure to 17α ethinylestradiol	E MTAB 5173:IonXpressRNA 004 up 058 4 s	IonXpressRNA 004 up 058 4 s	RNA seq of the zebrafish danio rerio brain post developmental exposure to 17α ethinylestradiol	RNA extraction Whole brains homogenized in TriReagent according to the manufacturer 0.8 ml/sample Sigma Aldrich Germany EE2 exposure. Fish exposed to 0 2.14 or 7.34 ng/L EE2 for 80 days starting 1 dpf Ion Whole transcriptome analysis kit on RiboZero eukaryote depleted total RNA	Experimental Factor: female:sex\|Experimental Factor: 17 ethinylestradiol:compound\|Experimental Factor: 7.34:dose	RNA-Seq	TRANSCRIPTOMIC	RANDOM	SINGLE	ION_TORRENT	Ion Torrent Proton		ERP018188	Ion Torrent Proton sequencing; RNA seq of the zebrafish danio rerio brain post developmental exposure to 17Î± ethinylestradiol	ENA FIRST PUBLIC:2017 10 02\|ENA LAST UPDATE:2018 11 16	IonXpressRNA_004_up_058_4.fastq.gz	fastq	2989727396.0	37953454.0	E MTAB 5173:IonXpressRNA 004 up 058 4	0:78.77	A:802963810;C:722434772;G:742850530;T:721478284;N:0	78	802963810	722434772	742850530	721478284	0	ERX1767852	ERS1417526	ERA739176	School of Natural Science, Technology and Environmental Studies Sodertorn University\|European Nucleotide Archive	School of Natural Science, Technology and Environmental Studies Sodertorn University\|European Nucleotide Archive	1	0.76402	0.32503	0.74241	0.4815	128	B	usable mapping rate	ion_torrent	ion_torrent	full_length	random_priming	ribozero	bulk	unknown	unknown	Sweden	2016-10-19	Pharyngula	Embryo	Brain	Nervous System
5804	5804	ERR1698343	ERX1767851	ERS1417525	ERP018188	PRJEB16335	RNA seq of the zebrafish danio rerio brain post developmental exposure to 17a ethinylestradiol	E-MTAB-5173	Transcriptome Analysis	17a ethinylestradiol EE2 is a synthetic estrogen commonly used as an active substance in oral contraceptives. It is frequently found in waste water effluent and raise concern due to its persistent nature. EE2 binds to estrogen receptors with similar affinity to oestradiol and acts as one of the most potent hormone mimics found in the environment. Estrogen is involved in many aspects of the development of the neuroendocrine system influencing both brain structure and behavior. We and others have reported a significant effect on non reproductive behaviors in adult fish and in recent studies we found that developmental exposure to EE2 resulted in an anxiogenic phenotype as adults even post a long remediation period. In this study we aim to study possible mechanisms behind the behavior alterations of zebrafish developmentally exposed to EE2 by sequencing the whole brain transcriptome. Zebrafish embryos were exposed to 0 2.14 and 7.34 ng/L EE2 from 1 day to 80 dpf post the exposure period a remediation period of 120 days followed before the fish were sampled. 3 male brains from the control group 0 ng/L and the 2.14 ng/L group were sampled and 3 female brains from the control group 0 ng/L and 7.34 ng/L were sampled.	ENA FIRST PUBLIC:2017 10 02\|ENA LAST UPDATE:2016 10 19\|ArrayExpress:E MTAB 5173	Protocols: RNA extraction Whole brains homogenized in TriReagent according to the manufacturer 0.8 ml/sample Sigma Aldrich Germany EE2 exposure. Fish exposed to 0 2.14 or 7.34 ng/L EE2 for 80 days starting 1 dpf Ion Whole transcriptome analysis kit on RiboZero eukaryote depleted total RNA	IonXpressRNA 003 up 058 3	SAMEA4518346	School of Natural Science, Technology and Environmental Studies Sodertorn University	ENA first public:2017 10 02\|ENA last update:2016 10 19\|External Id:SAMEA4518346\|INSDC center alias:School of Natural Science Technology and Environmental Studies Sodertorn University\|INSDC center name:School of Natural Science Technology and Environmental Studies Sodertorn University\|INSDC first public:2017 10 02T17:05:16Z\|INSDC last update:2016 10 19T13:53:59Z\|INSDC status:public\|Submitter Id:E MTAB 5173:IonXpressRNA 003 up 058 3\|age:200\|broker name:ArrayExpress\|common name:zebrafish\|organism part:brain\|sample name:E MTAB 5173:IonXpressRNA 003 up 058 3\|sex:female	Ion Torrent Proton sequencing; RNA seq of the zebrafish danio rerio brain post developmental exposure to 17α ethinylestradiol	E MTAB 5173:IonXpressRNA 003 up 058 3 s	IonXpressRNA 003 up 058 3 s	RNA seq of the zebrafish danio rerio brain post developmental exposure to 17α ethinylestradiol	RNA extraction Whole brains homogenized in TriReagent according to the manufacturer 0.8 ml/sample Sigma Aldrich Germany EE2 exposure. Fish exposed to 0 2.14 or 7.34 ng/L EE2 for 80 days starting 1 dpf Ion Whole transcriptome analysis kit on RiboZero eukaryote depleted total RNA	Experimental Factor: female:sex\|Experimental Factor: n1:compound	RNA-Seq	TRANSCRIPTOMIC	RANDOM	SINGLE	ION_TORRENT	Ion Torrent Proton		ERP018188	Ion Torrent Proton sequencing; RNA seq of the zebrafish danio rerio brain post developmental exposure to 17Î± ethinylestradiol	ENA FIRST PUBLIC:2017 10 02\|ENA LAST UPDATE:2018 11 16	IonXpressRNA_003_up_058_3.fastq.gz	fastq	4068383115.0	41996847.0	E MTAB 5173:IonXpressRNA 003 up 058 3	0:96.87	A:1133167706;C:944306379;G:948626944;T:1042282086;N:0	96	1133167706	944306379	948626944	1042282086	0	ERX1767851	ERS1417525	ERA739176	School of Natural Science, Technology and Environmental Studies Sodertorn University\|European Nucleotide Archive	School of Natural Science, Technology and Environmental Studies Sodertorn University\|European Nucleotide Archive	1	0.54804	0.22334	0.76353	0.49163	93	B	usable mapping rate	ion_torrent	ion_torrent	full_length	random_priming	ribozero	bulk	unknown	unknown	Sweden	2016-10-19	Pharyngula	Embryo	Brain	Nervous System
5805	5805	ERR1698342	ERX1767850	ERS1417524	ERP018188	PRJEB16335	RNA seq of the zebrafish danio rerio brain post developmental exposure to 17a ethinylestradiol	E-MTAB-5173	Transcriptome Analysis	17a ethinylestradiol EE2 is a synthetic estrogen commonly used as an active substance in oral contraceptives. It is frequently found in waste water effluent and raise concern due to its persistent nature. EE2 binds to estrogen receptors with similar affinity to oestradiol and acts as one of the most potent hormone mimics found in the environment. Estrogen is involved in many aspects of the development of the neuroendocrine system influencing both brain structure and behavior. We and others have reported a significant effect on non reproductive behaviors in adult fish and in recent studies we found that developmental exposure to EE2 resulted in an anxiogenic phenotype as adults even post a long remediation period. In this study we aim to study possible mechanisms behind the behavior alterations of zebrafish developmentally exposed to EE2 by sequencing the whole brain transcriptome. Zebrafish embryos were exposed to 0 2.14 and 7.34 ng/L EE2 from 1 day to 80 dpf post the exposure period a remediation period of 120 days followed before the fish were sampled. 3 male brains from the control group 0 ng/L and the 2.14 ng/L group were sampled and 3 female brains from the control group 0 ng/L and 7.34 ng/L were sampled.	ENA FIRST PUBLIC:2017 10 02\|ENA LAST UPDATE:2016 10 19\|ArrayExpress:E MTAB 5173	Protocols: RNA extraction Whole brains homogenized in TriReagent according to the manufacturer 0.8 ml/sample Sigma Aldrich Germany EE2 exposure. Fish exposed to 0 2.14 or 7.34 ng/L EE2 for 80 days starting 1 dpf Ion Whole transcriptome analysis kit on RiboZero eukaryote depleted total RNA	IonXpressRNA 002 up 058 2	SAMEA4518345	School of Natural Science, Technology and Environmental Studies Sodertorn University	ENA first public:2017 10 02\|ENA last update:2016 10 19\|External Id:SAMEA4518345\|INSDC center alias:School of Natural Science Technology and Environmental Studies Sodertorn University\|INSDC center name:School of Natural Science Technology and Environmental Studies Sodertorn University\|INSDC first public:2017 10 02T17:05:16Z\|INSDC last update:2016 10 19T13:53:59Z\|INSDC status:public\|Submitter Id:E MTAB 5173:IonXpressRNA 002 up 058 2\|age:200\|broker name:ArrayExpress\|common name:zebrafish\|organism part:brain\|sample name:E MTAB 5173:IonXpressRNA 002 up 058 2\|sex:female	Ion Torrent Proton sequencing; RNA seq of the zebrafish danio rerio brain post developmental exposure to 17α ethinylestradiol	E MTAB 5173:IonXpressRNA 002 up 058 2 s	IonXpressRNA 002 up 058 2 s	RNA seq of the zebrafish danio rerio brain post developmental exposure to 17α ethinylestradiol	RNA extraction Whole brains homogenized in TriReagent according to the manufacturer 0.8 ml/sample Sigma Aldrich Germany EE2 exposure. Fish exposed to 0 2.14 or 7.34 ng/L EE2 for 80 days starting 1 dpf Ion Whole transcriptome analysis kit on RiboZero eukaryote depleted total RNA	Experimental Factor: female:sex\|Experimental Factor: n1:compound	RNA-Seq	TRANSCRIPTOMIC	RANDOM	SINGLE	ION_TORRENT	Ion Torrent Proton		ERP018188	Ion Torrent Proton sequencing; RNA seq of the zebrafish danio rerio brain post developmental exposure to 17Î± ethinylestradiol	ENA FIRST PUBLIC:2017 10 02\|ENA LAST UPDATE:2018 11 16	IonXpressRNA_002_up_058_2.fastq.gz	fastq	2679963893.0	33552723.0	E MTAB 5173:IonXpressRNA 002 up 058 2	0:79.87	A:709092431;C:635329399;G:670478210;T:665063853;N:0	79	709092431	635329399	670478210	665063853	0	ERX1767850	ERS1417524	ERA739176	School of Natural Science, Technology and Environmental Studies Sodertorn University\|European Nucleotide Archive	School of Natural Science, Technology and Environmental Studies Sodertorn University\|European Nucleotide Archive	1	0.43283	0.18649	0.81178	0.52885	116	B	usable mapping rate	ion_torrent	ion_torrent	full_length	random_priming	ribozero	bulk	unknown	unknown	Sweden	2016-10-19	Pharyngula	Embryo	Brain	Nervous System
5806	5806	ERR1698341	ERX1767849	ERS1417523	ERP018188	PRJEB16335	RNA seq of the zebrafish danio rerio brain post developmental exposure to 17a ethinylestradiol	E-MTAB-5173	Transcriptome Analysis	17a ethinylestradiol EE2 is a synthetic estrogen commonly used as an active substance in oral contraceptives. It is frequently found in waste water effluent and raise concern due to its persistent nature. EE2 binds to estrogen receptors with similar affinity to oestradiol and acts as one of the most potent hormone mimics found in the environment. Estrogen is involved in many aspects of the development of the neuroendocrine system influencing both brain structure and behavior. We and others have reported a significant effect on non reproductive behaviors in adult fish and in recent studies we found that developmental exposure to EE2 resulted in an anxiogenic phenotype as adults even post a long remediation period. In this study we aim to study possible mechanisms behind the behavior alterations of zebrafish developmentally exposed to EE2 by sequencing the whole brain transcriptome. Zebrafish embryos were exposed to 0 2.14 and 7.34 ng/L EE2 from 1 day to 80 dpf post the exposure period a remediation period of 120 days followed before the fish were sampled. 3 male brains from the control group 0 ng/L and the 2.14 ng/L group were sampled and 3 female brains from the control group 0 ng/L and 7.34 ng/L were sampled.	ENA FIRST PUBLIC:2017 10 02\|ENA LAST UPDATE:2016 10 19\|ArrayExpress:E MTAB 5173	Protocols: RNA extraction Whole brains homogenized in TriReagent according to the manufacturer 0.8 ml/sample Sigma Aldrich Germany EE2 exposure. Fish exposed to 0 2.14 or 7.34 ng/L EE2 for 80 days starting 1 dpf Ion Whole transcriptome analysis kit on RiboZero eukaryote depleted total RNA	IonXpressRNA 001 up 058 1	SAMEA4518344	School of Natural Science, Technology and Environmental Studies Sodertorn University	ENA first public:2017 10 02\|ENA last update:2016 10 19\|External Id:SAMEA4518344\|INSDC center alias:School of Natural Science Technology and Environmental Studies Sodertorn University\|INSDC center name:School of Natural Science Technology and Environmental Studies Sodertorn University\|INSDC first public:2017 10 02T17:05:16Z\|INSDC last update:2016 10 19T13:53:59Z\|INSDC status:public\|Submitter Id:E MTAB 5173:IonXpressRNA 001 up 058 1\|age:200\|broker name:ArrayExpress\|common name:zebrafish\|organism part:brain\|sample name:E MTAB 5173:IonXpressRNA 001 up 058 1\|sex:female	Ion Torrent Proton sequencing; RNA seq of the zebrafish danio rerio brain post developmental exposure to 17α ethinylestradiol	E MTAB 5173:IonXpressRNA 001 up 058 1 s	IonXpressRNA 001 up 058 1 s	RNA seq of the zebrafish danio rerio brain post developmental exposure to 17α ethinylestradiol	RNA extraction Whole brains homogenized in TriReagent according to the manufacturer 0.8 ml/sample Sigma Aldrich Germany EE2 exposure. Fish exposed to 0 2.14 or 7.34 ng/L EE2 for 80 days starting 1 dpf Ion Whole transcriptome analysis kit on RiboZero eukaryote depleted total RNA	Experimental Factor: female:sex\|Experimental Factor: n1:compound	RNA-Seq	TRANSCRIPTOMIC	RANDOM	SINGLE	ION_TORRENT	Ion Torrent Proton		ERP018188	Ion Torrent Proton sequencing; RNA seq of the zebrafish danio rerio brain post developmental exposure to 17Î± ethinylestradiol	ENA FIRST PUBLIC:2017 10 02\|ENA LAST UPDATE:2018 11 16	IonXpressRNA_001_up_058_1.fastq.gz	fastq	3358902499.0	39857024.0	E MTAB 5173:IonXpressRNA 001 up 058 1	0:84.27	A:933405205;C:784111716;G:780404241;T:860981337;N:0	84	933405205	784111716	780404241	860981337	0	ERX1767849	ERS1417523	ERA739176	School of Natural Science, Technology and Environmental Studies Sodertorn University\|European Nucleotide Archive	School of Natural Science, Technology and Environmental Studies Sodertorn University\|European Nucleotide Archive	1	0.71789	0.33159	0.76619	0.514	56	B	usable mapping rate	ion_torrent	ion_torrent	full_length	random_priming	ribozero	bulk	unknown	unknown	Sweden	2016-10-19	Pharyngula	Embryo	Brain	Nervous System
39827	39827	SRR2441454	SRX1321833	SRS1109642	SRP062493	PRJNA293106	Danio rerio Raw sequence reads	PRJNA293106	Metagenomics	Zebrafish Hypoxia RNAseq		RNAseq at 24hpf following hypoxia		24hpf hypoxia		strain:TU\|age:24hpf\|sex:not determined\|tissue:brain\|treatment:hypoxia\|BioSampleModel:Model organism or animal	Zebrafish Embryonic Development Hypoxia Versus Normoxia RNAseq: Sample 24hpf hypoxia	24hpf.hypoxia.3.10329X21	24hpf.hypoxia.3.10329X21	We collected whole RNA from zebrafish at five different embryonic developmental stages with experimental triplicates for each stage time point and experimental condition. For hypoxia we placed developing embryos in pre conditioned media in a sealed plexiglass chamber set to a reduced oxygen concentration for 12 hour periods. Immediately following hypoxia or normoxia embryos were sacrificed and RNA collected. We used 1% pO2 for ages up to 48 hpf and 4% pO2 for older ages. At the time point for collection embryos were dissected and only head tissue was used for subsequent RNA preparation. RNA was prepared with a miRNeasy kit Qiagen. Quality of total RNAs was checked using a NanoDrop 2000 Spectrometer and an Agilent 2100 Bioanalyzer. The sequencing libraries were created using the Illumina TruSeq Stranded Total RNA Sample Preparation Kit with Ribo Zero. These libraries were sequenced on an Illumina HiSeq2000 sequencer for 50 cycles ie. 50 nucleotides single end stranded using Illumina’s version 3 chemistry. 6 samples were multiplexed together in each lane of the sequencer providing an average yield of 25.1 million reads per sample. The base calling was performed using Illumina’s CASAVA version 1.8.2 software.			RNA-Seq	TRANSCRIPTOMIC	RANDOM	SINGLE	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>50</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	SRP062493			10329X21_130822_SN141_0719_AD2CK5ACXX_7.txt.gz	fastq	1205447950.0	24108959.0	24hpf.hypoxia.3.10329X21	0:50	A:299680333;C:267717935;G:264833481;T:357936794;N:15279407	50	299680333	267717935	264833481	357936794	15279407	SRX1321833	SRS1109642	SRA289450	University of Utah\|Bonkowsky	University of Utah	1	0.87455	0.24266	0.74121	0.57777	50	B	usable mapping rate	illumina	hiseq_era	unknown	random_priming	trueseq	bulk	unknown	unknown	United States	2015-10-10	Pharyngula	Embryo	Brain	Nervous System
39828	39828	SRR2437828	SRX1321832	SRS1109643	SRP062493	PRJNA293106	Danio rerio Raw sequence reads	PRJNA293106	Metagenomics	Zebrafish Hypoxia RNAseq		RNAseq at 48hpf following normoxia		48hpf normoxia		strain:TU\|age:48hpf\|sex:not determined\|tissue:brain\|treatment:normoxia\|BioSampleModel:Model organism or animal	Zebrafish Embryonic Development Hypoxia Versus Normoxia RNAseq: Sample 48hpf normoxia	48hpf.normoxia.3.10329X16	48hpf.normoxia.3.10329X16	We collected whole RNA from zebrafish at five different embryonic developmental stages with experimental triplicates for each stage time point and experimental condition. For hypoxia we placed developing embryos in pre conditioned media in a sealed plexiglass chamber set to a reduced oxygen concentration for 12 hour periods. Immediately following hypoxia or normoxia embryos were sacrificed and RNA collected. We used 1% pO2 for ages up to 48 hpf and 4% pO2 for older ages. At the time point for collection embryos were dissected and only head tissue was used for subsequent RNA preparation. RNA was prepared with a miRNeasy kit Qiagen. Quality of total RNAs was checked using a NanoDrop 2000 Spectrometer and an Agilent 2100 Bioanalyzer. The sequencing libraries were created using the Illumina TruSeq Stranded Total RNA Sample Preparation Kit with Ribo Zero. These libraries were sequenced on an Illumina HiSeq2000 sequencer for 50 cycles ie. 50 nucleotides single end stranded using Illumina’s version 3 chemistry. 6 samples were multiplexed together in each lane of the sequencer providing an average yield of 25.1 million reads per sample. The base calling was performed using Illumina’s CASAVA version 1.8.2 software.			RNA-Seq	TRANSCRIPTOMIC	RANDOM	SINGLE	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>50</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	SRP062493			10329X16_130822_SN141_0719_AD2CK5ACXX_6.txt.gz	fastq	985942500.0	19718850.0	48hpf.normoxia.3.10329X16	0:50	A:251856100;C:223081125;G:216430606;T:294554748;N:19921	50	251856100	223081125	216430606	294554748	19921	SRX1321832	SRS1109643	SRA289450	University of Utah\|Bonkowsky	University of Utah	1	0.94791	0.26704	0.68708	0.54445	50	B	usable mapping rate	illumina	hiseq_era	unknown	random_priming	trueseq	bulk	unknown	unknown	United States	2015-10-10	Hatching	Embryo	Brain	Nervous System
39829	39829	SRR2434699	SRX1321831	SRS1109642	SRP062493	PRJNA293106	Danio rerio Raw sequence reads	PRJNA293106	Metagenomics	Zebrafish Hypoxia RNAseq		RNAseq at 24hpf following hypoxia		24hpf hypoxia		strain:TU\|age:24hpf\|sex:not determined\|tissue:brain\|treatment:hypoxia\|BioSampleModel:Model organism or animal	Zebrafish Embryonic Development Hypoxia Versus Normoxia RNAseq: Sample 24hpf hypoxia	24hpf.hypoxia.2.10329X11	24hpf.hypoxia.2.10329X11	We collected whole RNA from zebrafish at five different embryonic developmental stages with experimental triplicates for each stage time point and experimental condition. For hypoxia we placed developing embryos in pre conditioned media in a sealed plexiglass chamber set to a reduced oxygen concentration for 12 hour periods. Immediately following hypoxia or normoxia embryos were sacrificed and RNA collected. We used 1% pO2 for ages up to 48 hpf and 4% pO2 for older ages. At the time point for collection embryos were dissected and only head tissue was used for subsequent RNA preparation. RNA was prepared with a miRNeasy kit Qiagen. Quality of total RNAs was checked using a NanoDrop 2000 Spectrometer and an Agilent 2100 Bioanalyzer. The sequencing libraries were created using the Illumina TruSeq Stranded Total RNA Sample Preparation Kit with Ribo Zero. These libraries were sequenced on an Illumina HiSeq2000 sequencer for 50 cycles ie. 50 nucleotides single end stranded using Illumina’s version 3 chemistry. 6 samples were multiplexed together in each lane of the sequencer providing an average yield of 25.1 million reads per sample. The base calling was performed using Illumina’s CASAVA version 1.8.2 software.			RNA-Seq	TRANSCRIPTOMIC	RANDOM	SINGLE	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>50</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	SRP062493			10329X11_130822_SN141_0719_AD2CK5ACXX_5.txt.gz	fastq	1227682750.0	24553655.0	24hpf.hypoxia.2.10329X11	0:50	A:299759613;C:292757497;G:281904026;T:353193075;N:68539	50	299759613	292757497	281904026	353193075	68539	SRX1321831	SRS1109642	SRA289450	University of Utah\|Bonkowsky	University of Utah	1	0.94855	0.27579	0.73298	0.58307	50	B	usable mapping rate	illumina	hiseq_era	unknown	random_priming	trueseq	bulk	unknown	unknown	United States	2015-10-07	Pharyngula	Embryo	Brain	Nervous System
39830	39830	SRR2433795	SRX1321830	SRS1109644	SRP062493	PRJNA293106	Danio rerio Raw sequence reads	PRJNA293106	Metagenomics	Zebrafish Hypoxia RNAseq		RNAseq at 60hpf following normoxia		60hpf Normoxia		strain:TU\|age:60hpf\|sex:not determined\|tissue:brain\|treatment:normoxia\|BioSampleModel:Model organism or animal	Zebrafish Embryonic Development Hypoxia Versus Normoxia RNAseq: Sample 60hpf Normoxia	60hpf.normoxia.3.	60hpf.normoxia.3.	We collected whole RNA from zebrafish at five different embryonic developmental stages with experimental triplicates for each stage time point and experimental condition. For hypoxia we placed developing embryos in pre conditioned media in a sealed plexiglass chamber set to a reduced oxygen concentration for 12 hour periods. Immediately following hypoxia or normoxia embryos were sacrificed and RNA collected. We used 1% pO2 for ages up to 48 hpf and 4% pO2 for older ages. At the time point for collection embryos were dissected and only head tissue was used for subsequent RNA preparation. RNA was prepared with a miRNeasy kit Qiagen. Quality of total RNAs was checked using a NanoDrop 2000 Spectrometer and an Agilent 2100 Bioanalyzer. The sequencing libraries were created using the Illumina TruSeq Stranded Total RNA Sample Preparation Kit with Ribo Zero. These libraries were sequenced on an Illumina HiSeq2000 sequencer for 50 cycles ie. 50 nucleotides single end stranded using Illumina’s version 3 chemistry. 6 samples were multiplexed together in each lane of the sequencer providing an average yield of 25.1 million reads per sample. The base calling was performed using Illumina’s CASAVA version 1.8.2 software.			RNA-Seq	TRANSCRIPTOMIC	RANDOM	SINGLE	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>50</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	SRP062493			10329X8_130822_SN141_0719_AD2CK5ACXX_5.txt.gz	fastq	1112799950.0	22255999.0	60hpf.normoxia.3.	0:50	A:294304558;C:248234752;G:238088577;T:332109252;N:62811	50	294304558	248234752	238088577	332109252	62811	SRX1321830	SRS1109644	SRA289450	University of Utah\|Bonkowsky	University of Utah	1	0.94922	0.34314	0.67838	0.51455	50	B	usable mapping rate	illumina	hiseq_era	unknown	random_priming	trueseq	bulk	unknown	unknown	United States	2015-10-10	Hatching	Embryo	Brain	Nervous System
39831	39831	SRR2426760	SRX1321829	SRS1109645	SRP062493	PRJNA293106	Danio rerio Raw sequence reads	PRJNA293106	Metagenomics	Zebrafish Hypoxia RNAseq		RNAseq at 36hpf following hypoxia		36hpf hypoxia		strain:TU\|age:36hpf\|sex:not determined\|tissue:brain\|treatment:hypoxia\|BioSampleModel:Model organism or animal	Zebrafish Embryonic Development Hypoxia Versus Normoxia RNAseq: Sample 36hpf hypoxia	36hpf.hypoxia.1.10329X4	36hpf.hypoxia.1.10329X4	We collected whole RNA from zebrafish at five different embryonic developmental stages with experimental triplicates for each stage time point and experimental condition. For hypoxia we placed developing embryos in pre conditioned media in a sealed plexiglass chamber set to a reduced oxygen concentration for 12 hour periods. Immediately following hypoxia or normoxia embryos were sacrificed and RNA collected. We used 1% pO2 for ages up to 48 hpf and 4% pO2 for older ages. At the time point for collection embryos were dissected and only head tissue was used for subsequent RNA preparation. RNA was prepared with a miRNeasy kit Qiagen. Quality of total RNAs was checked using a NanoDrop 2000 Spectrometer and an Agilent 2100 Bioanalyzer. The sequencing libraries were created using the Illumina TruSeq Stranded Total RNA Sample Preparation Kit with Ribo Zero. These libraries were sequenced on an Illumina HiSeq2000 sequencer for 50 cycles ie. 50 nucleotides single end stranded using Illumina’s version 3 chemistry. 6 samples were multiplexed together in each lane of the sequencer providing an average yield of 25.1 million reads per sample. The base calling was performed using Illumina’s CASAVA version 1.8.2 software.			RNA-Seq	TRANSCRIPTOMIC	RANDOM	SINGLE	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>50</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	SRP062493			10329X4_130822_SN141_0719_AD2CK5ACXX_4.txt.gz	fastq	1604107450.0	32082149.0	36hpf.hypoxia.1.10329X4	0:50	A:395786409;C:358263833;G:358391489;T:491601452;N:64267	50	395786409	358263833	358391489	491601452	64267	SRX1321829	SRS1109645	SRA289450	University of Utah\|Bonkowsky	University of Utah	1	0.95408	0.28965	0.69593	0.54826	50	B	usable mapping rate	illumina	hiseq_era	unknown	random_priming	trueseq	bulk	unknown	unknown	United States	2015-10-10	Pharyngula	Embryo	Brain	Nervous System
39832	39832	SRR2417497	SRX1321828	SRS1109646	SRP062493	PRJNA293106	Danio rerio Raw sequence reads	PRJNA293106	Metagenomics	Zebrafish Hypoxia RNAseq		RNAseq at 48hpf following hypoxia		48hpf hypoxia		strain:TU\|age:48hpf\|sex:not determined\|tissue:brain\|treatment:hypoxia\|BioSampleModel:Model organism or animal	Zebrafish Embryonic Development Hypoxia Versus Normoxia RNAseq: Sample 48hpf hypoxia	48hpf.hypoxia.3.10329X25	48hpf.hypoxia.3.10329X25	We collected whole RNA from zebrafish at five different embryonic developmental stages with experimental triplicates for each stage time point and experimental condition. For hypoxia we placed developing embryos in pre conditioned media in a sealed plexiglass chamber set to a reduced oxygen concentration for 12 hour periods. Immediately following hypoxia or normoxia embryos were sacrificed and RNA collected. We used 1% pO2 for ages up to 48 hpf and 4% pO2 for older ages. At the time point for collection embryos were dissected and only head tissue was used for subsequent RNA preparation. RNA was prepared with a miRNeasy kit Qiagen. Quality of total RNAs was checked using a NanoDrop 2000 Spectrometer and an Agilent 2100 Bioanalyzer. The sequencing libraries were created using the Illumina TruSeq Stranded Total RNA Sample Preparation Kit with Ribo Zero. These libraries were sequenced on an Illumina HiSeq2000 sequencer for 50 cycles ie. 50 nucleotides single end stranded using Illumina’s version 3 chemistry. 6 samples were multiplexed together in each lane of the sequencer providing an average yield of 25.1 million reads per sample. The base calling was performed using Illumina’s CASAVA version 1.8.2 software.			RNA-Seq	TRANSCRIPTOMIC	RANDOM	SINGLE	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>50</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	SRP062493			10329X25_130822_SN141_0719_AD2CK5ACXX_8.txt.gz	fastq	1234710500.0	24694210.0	48hpf.hypoxia.3.10329X25	0:50	A:309392529;C:284311489;G:275471070;T:365515358;N:20054	50	309392529	284311489	275471070	365515358	20054	SRX1321828	SRS1109646	SRA289450	University of Utah\|Bonkowsky	University of Utah	1	0.94675	0.25877	0.71453	0.5239	50	B	usable mapping rate	illumina	hiseq_era	unknown	random_priming	trueseq	bulk	unknown	unknown	United States	2015-09-16	Hatching	Embryo	Brain	Nervous System
39833	39833	SRR2417496	SRX1321827	SRS1109644	SRP062493	PRJNA293106	Danio rerio Raw sequence reads	PRJNA293106	Metagenomics	Zebrafish Hypoxia RNAseq		RNAseq at 60hpf following normoxia		60hpf Normoxia		strain:TU\|age:60hpf\|sex:not determined\|tissue:brain\|treatment:normoxia\|BioSampleModel:Model organism or animal	Zebrafish Embryonic Development Hypoxia Versus Normoxia RNAseq: Sample 60hpf Normoxia	60hpf.normoxia.2.10329X28	60hpf.normoxia.2.10329X28	We collected whole RNA from zebrafish at five different embryonic developmental stages with experimental triplicates for each stage time point and experimental condition. For hypoxia we placed developing embryos in pre conditioned media in a sealed plexiglass chamber set to a reduced oxygen concentration for 12 hour periods. Immediately following hypoxia or normoxia embryos were sacrificed and RNA collected. We used 1% pO2 for ages up to 48 hpf and 4% pO2 for older ages. At the time point for collection embryos were dissected and only head tissue was used for subsequent RNA preparation. RNA was prepared with a miRNeasy kit Qiagen. Quality of total RNAs was checked using a NanoDrop 2000 Spectrometer and an Agilent 2100 Bioanalyzer. The sequencing libraries were created using the Illumina TruSeq Stranded Total RNA Sample Preparation Kit with Ribo Zero. These libraries were sequenced on an Illumina HiSeq2000 sequencer for 50 cycles ie. 50 nucleotides single end stranded using Illumina’s version 3 chemistry. 6 samples were multiplexed together in each lane of the sequencer providing an average yield of 25.1 million reads per sample. The base calling was performed using Illumina’s CASAVA version 1.8.2 software.			RNA-Seq	TRANSCRIPTOMIC	RANDOM	SINGLE	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>50</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	SRP062493			10329X28_130822_SN141_0719_AD2CK5ACXX_8.txt.gz	fastq	1015786750.0	20315735.0	60hpf.normoxia.2.10329X28	0:50	A:259102784;C:230364456;G:226008843;T:300294199;N:16468	50	259102784	230364456	226008843	300294199	16468	SRX1321827	SRS1109644	SRA289450	University of Utah\|Bonkowsky	University of Utah	1	0.95197	0.30503	0.68333	0.52243	50	B	usable mapping rate	illumina	hiseq_era	unknown	random_priming	trueseq	bulk	unknown	unknown	United States	2015-09-16	Hatching	Embryo	Brain	Nervous System
39834	39834	SRR2400601	SRX1321826	SRS1109642	SRP062493	PRJNA293106	Danio rerio Raw sequence reads	PRJNA293106	Metagenomics	Zebrafish Hypoxia RNAseq		RNAseq at 24hpf following hypoxia		24hpf hypoxia		strain:TU\|age:24hpf\|sex:not determined\|tissue:brain\|treatment:hypoxia\|BioSampleModel:Model organism or animal	Zebrafish Embryonic Development Hypoxia Versus Normoxia RNAseq: Sample 24hpf hypoxia	24hpf.hypoxia.1.10329X2	24hpf.hypoxia.1.10329X2	We collected whole RNA from zebrafish at five different embryonic developmental stages with experimental triplicates for each stage time point and experimental condition. For hypoxia we placed developing embryos in pre conditioned media in a sealed plexiglass chamber set to a reduced oxygen concentration for 12 hour periods. Immediately following hypoxia or normoxia embryos were sacrificed and RNA collected. We used 1% pO2 for ages up to 48 hpf and 4% pO2 for older ages. At the time point for collection embryos were dissected and only head tissue was used for subsequent RNA preparation. RNA was prepared with a miRNeasy kit Qiagen. Quality of total RNAs was checked using a NanoDrop 2000 Spectrometer and an Agilent 2100 Bioanalyzer. The sequencing libraries were created using the Illumina TruSeq Stranded Total RNA Sample Preparation Kit with Ribo Zero. These libraries were sequenced on an Illumina HiSeq2000 sequencer for 50 cycles ie. 50 nucleotides single end stranded using Illumina’s version 3 chemistry. 6 samples were multiplexed together in each lane of the sequencer providing an average yield of 25.1 million reads per sample. The base calling was performed using Illumina’s CASAVA version 1.8.2 software.			RNA-Seq	TRANSCRIPTOMIC	RANDOM	SINGLE	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>50</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	SRP062493			10329X2_130822_SN141_0719_AD2CK5ACXX_4.txt.gz	fastq	1249046350.0	24980927.0	24hpf.hypoxia.1.10329X2	0:50	A:309098695;C:281875016;G:278706888;T:379315616;N:50135	50	309098695	281875016	278706888	379315616	50135	SRX1321826	SRS1109642	SRA289450	University of Utah\|Bonkowsky	University of Utah	1	0.95443	0.27405	0.69747	0.40987	50	B	usable mapping rate	illumina	hiseq_era	unknown	random_priming	trueseq	bulk	unknown	unknown	United States	2015-10-10	Pharyngula	Embryo	Brain	Nervous System
39835	39835	SRR2382596	SRX1321825	SRS1109647	SRP062493	PRJNA293106	Danio rerio Raw sequence reads	PRJNA293106	Metagenomics	Zebrafish Hypoxia RNAseq		RNAseq at 72hpf following normoxia		72hpf normoxia		strain:TU\|age:72hpf\|sex:not determined\|tissue:brain\|treatment:normoxia\|BioSampleModel:Model organism or animal	Zebrafish Embryonic Development Hypoxia Versus Normoxia RNAseq: Sample 72hpf normoxia	72hpf.normoxia.3.10329X30	72hpf.normoxia.3.10329X30	We collected whole RNA from zebrafish at five different embryonic developmental stages with experimental triplicates for each stage time point and experimental condition. For hypoxia we placed developing embryos in pre conditioned media in a sealed plexiglass chamber set to a reduced oxygen concentration for 12 hour periods. Immediately following hypoxia or normoxia embryos were sacrificed and RNA collected. We used 1% pO2 for ages up to 48 hpf and 4% pO2 for older ages. At the time point for collection embryos were dissected and only head tissue was used for subsequent RNA preparation. RNA was prepared with a miRNeasy kit Qiagen. Quality of total RNAs was checked using a NanoDrop 2000 Spectrometer and an Agilent 2100 Bioanalyzer. The sequencing libraries were created using the Illumina TruSeq Stranded Total RNA Sample Preparation Kit with Ribo Zero. These libraries were sequenced on an Illumina HiSeq2000 sequencer for 50 cycles ie. 50 nucleotides single end stranded using Illumina’s version 3 chemistry. 6 samples were multiplexed together in each lane of the sequencer providing an average yield of 25.1 million reads per sample. The base calling was performed using Illumina’s CASAVA version 1.8.2 software.			RNA-Seq	TRANSCRIPTOMIC	RANDOM	SINGLE	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>50</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	SRP062493			10329X30_130822_SN141_0719_AD2CK5ACXX_8.txt.gz	fastq	1202543000.0	24050860.0	72hpf.normoxia.3.10329X30	0:50	A:317814132;C:265339479;G:263354488;T:356015292;N:19609	50	317814132	265339479	263354488	356015292	19609	SRX1321825	SRS1109647	SRA289450	University of Utah\|Bonkowsky	University of Utah	1	0.94396	0.39792	0.68592	0.51696	50	B	usable mapping rate	illumina	hiseq_era	unknown	random_priming	trueseq	bulk	unknown	unknown	United States	2015-10-10	Larval	Larval	Brain	Nervous System
39836	39836	SRR2346975	SRX1321824	SRS1109649	SRP062493	PRJNA293106	Danio rerio Raw sequence reads	PRJNA293106	Metagenomics	Zebrafish Hypoxia RNAseq		RNAseq at 72hpf following hypoxia		72hpf hypoxia		strain:TU\|age:72hpf\|sex:not determined\|tissue:brain\|treatment:hypoxia\|BioSampleModel:Model organism or animal	Zebrafish Embryonic Development Hypoxia Versus Normoxia RNAseq: Sample 72hpf hypoxia	72hpf.hypoxia.3.10329X29	72hpf.hypoxia.3.10329X29	We collected whole RNA from zebrafish at five different embryonic developmental stages with experimental triplicates for each stage time point and experimental condition. For hypoxia we placed developing embryos in pre conditioned media in a sealed plexiglass chamber set to a reduced oxygen concentration for 12 hour periods. Immediately following hypoxia or normoxia embryos were sacrificed and RNA collected. We used 1% pO2 for ages up to 48 hpf and 4% pO2 for older ages. At the time point for collection embryos were dissected and only head tissue was used for subsequent RNA preparation. RNA was prepared with a miRNeasy kit Qiagen. Quality of total RNAs was checked using a NanoDrop 2000 Spectrometer and an Agilent 2100 Bioanalyzer. The sequencing libraries were created using the Illumina TruSeq Stranded Total RNA Sample Preparation Kit with Ribo Zero. These libraries were sequenced on an Illumina HiSeq2000 sequencer for 50 cycles ie. 50 nucleotides single end stranded using Illumina’s version 3 chemistry. 6 samples were multiplexed together in each lane of the sequencer providing an average yield of 25.1 million reads per sample. The base calling was performed using Illumina’s CASAVA version 1.8.2 software.			RNA-Seq	TRANSCRIPTOMIC	RANDOM	SINGLE	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>50</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	SRP062493			10329X29_130822_SN141_0719_AD2CK5ACXX_8.txt.gz	fastq	977694000.0	19553880.0	72hpf.hypoxia.3.10329X29	0:50	A:252845467;C:218082615;G:213237499;T:293512810;N:15609	50	252845467	218082615	213237499	293512810	15609	SRX1321824	SRS1109649	SRA289450	University of Utah\|Bonkowsky	University of Utah	1	0.94858	0.35206	0.68889	0.53177	50	B	usable mapping rate	illumina	hiseq_era	unknown	random_priming	trueseq	bulk	unknown	unknown	United States	2015-09-10	Larval	Larval	Brain	Nervous System
39837	39837	SRR2342161	SRX1321823	SRS1109648	SRP062493	PRJNA293106	Danio rerio Raw sequence reads	PRJNA293106	Metagenomics	Zebrafish Hypoxia RNAseq		RNAseq at 60hpf following hypoxia		60hpf hypoxia		strain:TU\|age:60hpf\|sex:not determined\|tissue:brain\|treatment:hypoxia\|BioSampleModel:Model organism or animal	Zebrafish Embryonic Development Hypoxia Versus Normoxia RNAseq: Sample 60hpf hypoxia	60hpf.hypoxia.3.10329X27	60hpf.hypoxia.3.10329X27	We collected whole RNA from zebrafish at five different embryonic developmental stages with experimental triplicates for each stage time point and experimental condition. For hypoxia we placed developing embryos in pre conditioned media in a sealed plexiglass chamber set to a reduced oxygen concentration for 12 hour periods. Immediately following hypoxia or normoxia embryos were sacrificed and RNA collected. We used 1% pO2 for ages up to 48 hpf and 4% pO2 for older ages. At the time point for collection embryos were dissected and only head tissue was used for subsequent RNA preparation. RNA was prepared with a miRNeasy kit Qiagen. Quality of total RNAs was checked using a NanoDrop 2000 Spectrometer and an Agilent 2100 Bioanalyzer. The sequencing libraries were created using the Illumina TruSeq Stranded Total RNA Sample Preparation Kit with Ribo Zero. These libraries were sequenced on an Illumina HiSeq2000 sequencer for 50 cycles ie. 50 nucleotides single end stranded using Illumina’s version 3 chemistry. 6 samples were multiplexed together in each lane of the sequencer providing an average yield of 25.1 million reads per sample. The base calling was performed using Illumina’s CASAVA version 1.8.2 software.			RNA-Seq	TRANSCRIPTOMIC	RANDOM	SINGLE	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>50</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	SRP062493			10329X27_130822_SN141_0719_AD2CK5ACXX_8.txt.gz	fastq	1073269500.0	21465390.0	60hpf.hypoxia.3.10329X27	0:50	A:280500644;C:237758835;G:234061287;T:320931292;N:17442	50	280500644	237758835	234061287	320931292	17442	SRX1321823	SRS1109648	SRA289450	University of Utah\|Bonkowsky	University of Utah	1	0.94774	0.36354	0.70065	0.51075	50	B	usable mapping rate	illumina	hiseq_era	unknown	random_priming	trueseq	bulk	unknown	unknown	United States	2015-10-10	Hatching	Embryo	Brain	Nervous System
39838	39838	SRR2341509	SRX1321822	SRS1109643	SRP062493	PRJNA293106	Danio rerio Raw sequence reads	PRJNA293106	Metagenomics	Zebrafish Hypoxia RNAseq		RNAseq at 48hpf following normoxia		48hpf normoxia		strain:TU\|age:48hpf\|sex:not determined\|tissue:brain\|treatment:normoxia\|BioSampleModel:Model organism or animal	Zebrafish Embryonic Development Hypoxia Versus Normoxia RNAseq: Sample 48hpf normoxia	48hpf.normoxia.210329X26	48hpf.normoxia.210329X26	We collected whole RNA from zebrafish at five different embryonic developmental stages with experimental triplicates for each stage time point and experimental condition. For hypoxia we placed developing embryos in pre conditioned media in a sealed plexiglass chamber set to a reduced oxygen concentration for 12 hour periods. Immediately following hypoxia or normoxia embryos were sacrificed and RNA collected. We used 1% pO2 for ages up to 48 hpf and 4% pO2 for older ages. At the time point for collection embryos were dissected and only head tissue was used for subsequent RNA preparation. RNA was prepared with a miRNeasy kit Qiagen. Quality of total RNAs was checked using a NanoDrop 2000 Spectrometer and an Agilent 2100 Bioanalyzer. The sequencing libraries were created using the Illumina TruSeq Stranded Total RNA Sample Preparation Kit with Ribo Zero. These libraries were sequenced on an Illumina HiSeq2000 sequencer for 50 cycles ie. 50 nucleotides single end stranded using Illumina’s version 3 chemistry. 6 samples were multiplexed together in each lane of the sequencer providing an average yield of 25.1 million reads per sample. The base calling was performed using Illumina’s CASAVA version 1.8.2 software.			RNA-Seq	TRANSCRIPTOMIC	RANDOM	SINGLE	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>50</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	SRP062493			10329X26_130822_SN141_0719_AD2CK5ACXX_8.txt.gz	fastq	1075868850.0	21517377.0	48hpf.normoxia.210329X26	0:50	A:271795722;C:246789566;G:240496674;T:316769688;N:17200	50	271795722	246789566	240496674	316769688	17200	SRX1321822	SRS1109643	SRA289450	University of Utah\|Bonkowsky	University of Utah	1	0.94644	0.26805	0.68745	0.53833	50	B	usable mapping rate	illumina	hiseq_era	unknown	random_priming	trueseq	bulk	unknown	unknown	United States	2015-10-10	Hatching	Embryo	Brain	Nervous System
39839	39839	SRR2340149	SRX1321821	SRS1109650	SRP062493	PRJNA293106	Danio rerio Raw sequence reads	PRJNA293106	Metagenomics	Zebrafish Hypoxia RNAseq		RNAseq at 36hpf following normoxia		36hpf normoxia		strain:TU\|age:36hpf\|sex:not determined\|tissue:brain\|treatment:normoxia\|BioSampleModel:Model organism or animal	Zebrafish Embryonic Development Hypoxia Versus Normoxia RNAseq: Sample 36hpf normoxia	36hpf.normoxia.3.10329X24	36hpf.normoxia.3.10329X24	We collected whole RNA from zebrafish at five different embryonic developmental stages with experimental triplicates for each stage time point and experimental condition. For hypoxia we placed developing embryos in pre conditioned media in a sealed plexiglass chamber set to a reduced oxygen concentration for 12 hour periods. Immediately following hypoxia or normoxia embryos were sacrificed and RNA collected. We used 1% pO2 for ages up to 48 hpf and 4% pO2 for older ages. At the time point for collection embryos were dissected and only head tissue was used for subsequent RNA preparation. RNA was prepared with a miRNeasy kit Qiagen. Quality of total RNAs was checked using a NanoDrop 2000 Spectrometer and an Agilent 2100 Bioanalyzer. The sequencing libraries were created using the Illumina TruSeq Stranded Total RNA Sample Preparation Kit with Ribo Zero. These libraries were sequenced on an Illumina HiSeq2000 sequencer for 50 cycles ie. 50 nucleotides single end stranded using Illumina’s version 3 chemistry. 6 samples were multiplexed together in each lane of the sequencer providing an average yield of 25.1 million reads per sample. The base calling was performed using Illumina’s CASAVA version 1.8.2 software.			RNA-Seq	TRANSCRIPTOMIC	RANDOM	SINGLE	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>50</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	SRP062493			10329X24_130822_SN141_0719_AD2CK5ACXX_7.txt.gz	fastq	1588630750.0	31772615.0	36hpf.normoxia.3.10329X24	0:50	A:404319446;C:349459823;G:346545846;T:468209953;N:20095682	50	404319446	349459823	346545846	468209953	20095682	SRX1321821	SRS1109650	SRA289450	University of Utah\|Bonkowsky	University of Utah	1	0.8739	0.32489	0.71701	0.51646	50	B	usable mapping rate	illumina	hiseq_era	unknown	random_priming	trueseq	bulk	unknown	unknown	United States	2015-10-10	Pharyngula	Embryo	Brain	Nervous System
39840	39840	SRR2245887	SRX1321820	SRS1109645	SRP062493	PRJNA293106	Danio rerio Raw sequence reads	PRJNA293106	Metagenomics	Zebrafish Hypoxia RNAseq		RNAseq at 36hpf following hypoxia		36hpf hypoxia		strain:TU\|age:36hpf\|sex:not determined\|tissue:brain\|treatment:hypoxia\|BioSampleModel:Model organism or animal	Zebrafish Embryonic Development Hypoxia Versus Normoxia RNAseq: Sample 36hpf hypoxia	36hpf.hypoxia.3.10329X23	36hpf.hypoxia.3.10329X23	We collected whole RNA from zebrafish at five different embryonic developmental stages with experimental triplicates for each stage time point and experimental condition. For hypoxia we placed developing embryos in pre conditioned media in a sealed plexiglass chamber set to a reduced oxygen concentration for 12 hour periods. Immediately following hypoxia or normoxia embryos were sacrificed and RNA collected. We used 1% pO2 for ages up to 48 hpf and 4% pO2 for older ages. At the time point for collection embryos were dissected and only head tissue was used for subsequent RNA preparation. RNA was prepared with a miRNeasy kit Qiagen. Quality of total RNAs was checked using a NanoDrop 2000 Spectrometer and an Agilent 2100 Bioanalyzer. The sequencing libraries were created using the Illumina TruSeq Stranded Total RNA Sample Preparation Kit with Ribo Zero. These libraries were sequenced on an Illumina HiSeq2000 sequencer for 50 cycles ie. 50 nucleotides single end stranded using Illumina’s version 3 chemistry. 6 samples were multiplexed together in each lane of the sequencer providing an average yield of 25.1 million reads per sample. The base calling was performed using Illumina’s CASAVA version 1.8.2 software.			RNA-Seq	TRANSCRIPTOMIC	RANDOM	SINGLE	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>50</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	SRP062493			10329X23_130822_SN141_0719_AD2CK5ACXX_7.txt.gz	fastq	1207331350.0	24146627.0	36hpf.hypoxia.3.10329X23	0:50	A:305628501;C:266059461;G:259888045;T:360487730;N:15267613	50	305628501	266059461	259888045	360487730	15267613	SRX1321820	SRS1109645	SRA289450	University of Utah\|Bonkowsky	University of Utah	1	0.87814	0.27249	0.72853	0.53527	50	B	usable mapping rate	illumina	hiseq_era	unknown	random_priming	trueseq	bulk	unknown	unknown	United States	2015-10-10	Pharyngula	Embryo	Brain	Nervous System
39841	39841	SRR2245257	SRX1321819	SRS1109651	SRP062493	PRJNA293106	Danio rerio Raw sequence reads	PRJNA293106	Metagenomics	Zebrafish Hypoxia RNAseq		RNAseq at 24hpf following normoxia		24hpf normoxia		strain:TU\|age:24hpf\|sex:not determined\|tissue:brain\|treatment:normoxia\|BioSampleModel:Model organism or animal	Zebrafish Embryonic Development Hypoxia Versus Normoxia RNAseq: Sample 24hpf normoxia	24hpf.normoxia.3.10329X22	24hpf.normoxia.3.10329X22	We collected whole RNA from zebrafish at five different embryonic developmental stages with experimental triplicates for each stage time point and experimental condition. For hypoxia we placed developing embryos in pre conditioned media in a sealed plexiglass chamber set to a reduced oxygen concentration for 12 hour periods. Immediately following hypoxia or normoxia embryos were sacrificed and RNA collected. We used 1% pO2 for ages up to 48 hpf and 4% pO2 for older ages. At the time point for collection embryos were dissected and only head tissue was used for subsequent RNA preparation. RNA was prepared with a miRNeasy kit Qiagen. Quality of total RNAs was checked using a NanoDrop 2000 Spectrometer and an Agilent 2100 Bioanalyzer. The sequencing libraries were created using the Illumina TruSeq Stranded Total RNA Sample Preparation Kit with Ribo Zero. These libraries were sequenced on an Illumina HiSeq2000 sequencer for 50 cycles ie. 50 nucleotides single end stranded using Illumina’s version 3 chemistry. 6 samples were multiplexed together in each lane of the sequencer providing an average yield of 25.1 million reads per sample. The base calling was performed using Illumina’s CASAVA version 1.8.2 software.			RNA-Seq	TRANSCRIPTOMIC	RANDOM	SINGLE	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>50</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	SRP062493			10329X22_130822_SN141_0719_AD2CK5ACXX_7.txt.gz	fastq	1257159050.0	25143181.0	24hpf.normoxia.3.10329X22	0:50	A:320558638;C:275312235;G:271710647;T:373637690;N:15939840	50	320558638	275312235	271710647	373637690	15939840	SRX1321819	SRS1109651	SRA289450	University of Utah\|Bonkowsky	University of Utah	1	0.87634	0.30298	0.71356	0.54673	50	B	usable mapping rate	illumina	hiseq_era	unknown	random_priming	trueseq	bulk	unknown	unknown	United States	2015-10-10	Pharyngula	Embryo	Brain	Nervous System
39842	39842	SRR2243358	SRX1321818	SRS1109647	SRP062493	PRJNA293106	Danio rerio Raw sequence reads	PRJNA293106	Metagenomics	Zebrafish Hypoxia RNAseq		RNAseq at 72hpf following normoxia		72hpf normoxia		strain:TU\|age:72hpf\|sex:not determined\|tissue:brain\|treatment:normoxia\|BioSampleModel:Model organism or animal	Zebrafish Embryonic Development Hypoxia Versus Normoxia RNAseq: Sample 72hpf normoxia	72hpf.normoxia.2.10329X20	72hpf.normoxia.2.10329X20	We collected whole RNA from zebrafish at five different embryonic developmental stages with experimental triplicates for each stage time point and experimental condition. For hypoxia we placed developing embryos in pre conditioned media in a sealed plexiglass chamber set to a reduced oxygen concentration for 12 hour periods. Immediately following hypoxia or normoxia embryos were sacrificed and RNA collected. We used 1% pO2 for ages up to 48 hpf and 4% pO2 for older ages. At the time point for collection embryos were dissected and only head tissue was used for subsequent RNA preparation. RNA was prepared with a miRNeasy kit Qiagen. Quality of total RNAs was checked using a NanoDrop 2000 Spectrometer and an Agilent 2100 Bioanalyzer. The sequencing libraries were created using the Illumina TruSeq Stranded Total RNA Sample Preparation Kit with Ribo Zero. These libraries were sequenced on an Illumina HiSeq2000 sequencer for 50 cycles ie. 50 nucleotides single end stranded using Illumina’s version 3 chemistry. 6 samples were multiplexed together in each lane of the sequencer providing an average yield of 25.1 million reads per sample. The base calling was performed using Illumina’s CASAVA version 1.8.2 software.			RNA-Seq	TRANSCRIPTOMIC	RANDOM	SINGLE	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>50</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	SRP062493			10329X20_130822_SN141_0719_AD2CK5ACXX_7.txt.gz	fastq	1530276050.0	30605521.0	72hpf.normoxia.2.10329X20	0:50	A:387360143;C:340062704;G:324515181;T:458965961;N:19372061	50	387360143	340062704	324515181	458965961	19372061	SRX1321818	SRS1109647	SRA289450	University of Utah\|Bonkowsky	University of Utah	1	0.8774	0.25469	0.68799	0.52777	50	B	usable mapping rate	illumina	hiseq_era	unknown	random_priming	trueseq	bulk	unknown	unknown	United States	2015-10-10	Larval	Larval	Brain	Nervous System
39843	39843	SRR2243161	SRX1321817	SRS1109649	SRP062493	PRJNA293106	Danio rerio Raw sequence reads	PRJNA293106	Metagenomics	Zebrafish Hypoxia RNAseq		RNAseq at 72hpf following hypoxia		72hpf hypoxia		strain:TU\|age:72hpf\|sex:not determined\|tissue:brain\|treatment:hypoxia\|BioSampleModel:Model organism or animal	Zebrafish Embryonic Development Hypoxia Versus Normoxia RNAseq: Sample 72hpf hypoxia	72hpf.hypoxia.2.10329X19	72hpf.hypoxia.2.10329X19	We collected whole RNA from zebrafish at five different embryonic developmental stages with experimental triplicates for each stage time point and experimental condition. For hypoxia we placed developing embryos in pre conditioned media in a sealed plexiglass chamber set to a reduced oxygen concentration for 12 hour periods. Immediately following hypoxia or normoxia embryos were sacrificed and RNA collected. We used 1% pO2 for ages up to 48 hpf and 4% pO2 for older ages. At the time point for collection embryos were dissected and only head tissue was used for subsequent RNA preparation. RNA was prepared with a miRNeasy kit Qiagen. Quality of total RNAs was checked using a NanoDrop 2000 Spectrometer and an Agilent 2100 Bioanalyzer. The sequencing libraries were created using the Illumina TruSeq Stranded Total RNA Sample Preparation Kit with Ribo Zero. These libraries were sequenced on an Illumina HiSeq2000 sequencer for 50 cycles ie. 50 nucleotides single end stranded using Illumina’s version 3 chemistry. 6 samples were multiplexed together in each lane of the sequencer providing an average yield of 25.1 million reads per sample. The base calling was performed using Illumina’s CASAVA version 1.8.2 software.			RNA-Seq	TRANSCRIPTOMIC	RANDOM	SINGLE	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>50</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	SRP062493			10329X19_130822_SN141_0719_AD2CK5ACXX_7.txt.gz	fastq	1866619850.0	37332397.0	72hpf.hypoxia.2.10329X19	0:50	A:465524152;C:423400277;G:418937882;T:535202326;N:23555213	50	465524152	423400277	418937882	535202326	23555213	SRX1321817	SRS1109649	SRA289450	University of Utah\|Bonkowsky	University of Utah	1	0.85514	0.26828	0.7041	0.54006	50	B	usable mapping rate	illumina	hiseq_era	unknown	random_priming	trueseq	bulk	unknown	unknown	United States	2015-10-10	Larval	Larval	Brain	Nervous System
39844	39844	SRR2241311	SRX1321816	SRS1109644	SRP062493	PRJNA293106	Danio rerio Raw sequence reads	PRJNA293106	Metagenomics	Zebrafish Hypoxia RNAseq		RNAseq at 60hpf following normoxia		60hpf Normoxia		strain:TU\|age:60hpf\|sex:not determined\|tissue:brain\|treatment:normoxia\|BioSampleModel:Model organism or animal	Zebrafish Embryonic Development Hypoxia Versus Normoxia RNAseq: Sample 60hpf Normoxia	60hpf.normoxia.1.10329X18	60hpf.normoxia.1.10329X18	We collected whole RNA from zebrafish at five different embryonic developmental stages with experimental triplicates for each stage time point and experimental condition. For hypoxia we placed developing embryos in pre conditioned media in a sealed plexiglass chamber set to a reduced oxygen concentration for 12 hour periods. Immediately following hypoxia or normoxia embryos were sacrificed and RNA collected. We used 1% pO2 for ages up to 48 hpf and 4% pO2 for older ages. At the time point for collection embryos were dissected and only head tissue was used for subsequent RNA preparation. RNA was prepared with a miRNeasy kit Qiagen. Quality of total RNAs was checked using a NanoDrop 2000 Spectrometer and an Agilent 2100 Bioanalyzer. The sequencing libraries were created using the Illumina TruSeq Stranded Total RNA Sample Preparation Kit with Ribo Zero. These libraries were sequenced on an Illumina HiSeq2000 sequencer for 50 cycles ie. 50 nucleotides single end stranded using Illumina’s version 3 chemistry. 6 samples were multiplexed together in each lane of the sequencer providing an average yield of 25.1 million reads per sample. The base calling was performed using Illumina’s CASAVA version 1.8.2 software.			RNA-Seq	TRANSCRIPTOMIC	RANDOM	SINGLE	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>50</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	SRP062493			10329X18_130822_SN141_0719_AD2CK5ACXX_6.txt.gz	fastq	1118115050.0	22362301.0	60hpf.normoxia.1.10329X18	0:50	A:287072563;C:255244675;G:248573701;T:327203388;N:20723	50	287072563	255244675	248573701	327203388	20723	SRX1321816	SRS1109644	SRA289450	University of Utah\|Bonkowsky	University of Utah	1	0.93238	0.30064	0.68574	0.51934	50	B	usable mapping rate	illumina	hiseq_era	unknown	random_priming	trueseq	bulk	unknown	unknown	United States	2015-10-10	Hatching	Embryo	Brain	Nervous System
39845	39845	SRR2240748	SRX1321815	SRS1109648	SRP062493	PRJNA293106	Danio rerio Raw sequence reads	PRJNA293106	Metagenomics	Zebrafish Hypoxia RNAseq		RNAseq at 60hpf following hypoxia		60hpf hypoxia		strain:TU\|age:60hpf\|sex:not determined\|tissue:brain\|treatment:hypoxia\|BioSampleModel:Model organism or animal	Zebrafish Embryonic Development Hypoxia Versus Normoxia RNAseq: Sample 60hpf hypoxia	60hpf.hypoxia.2.10329X17	60hpf.hypoxia.2.10329X17	We collected whole RNA from zebrafish at five different embryonic developmental stages with experimental triplicates for each stage time point and experimental condition. For hypoxia we placed developing embryos in pre conditioned media in a sealed plexiglass chamber set to a reduced oxygen concentration for 12 hour periods. Immediately following hypoxia or normoxia embryos were sacrificed and RNA collected. We used 1% pO2 for ages up to 48 hpf and 4% pO2 for older ages. At the time point for collection embryos were dissected and only head tissue was used for subsequent RNA preparation. RNA was prepared with a miRNeasy kit Qiagen. Quality of total RNAs was checked using a NanoDrop 2000 Spectrometer and an Agilent 2100 Bioanalyzer. The sequencing libraries were created using the Illumina TruSeq Stranded Total RNA Sample Preparation Kit with Ribo Zero. These libraries were sequenced on an Illumina HiSeq2000 sequencer for 50 cycles ie. 50 nucleotides single end stranded using Illumina’s version 3 chemistry. 6 samples were multiplexed together in each lane of the sequencer providing an average yield of 25.1 million reads per sample. The base calling was performed using Illumina’s CASAVA version 1.8.2 software.			RNA-Seq	TRANSCRIPTOMIC	RANDOM	SINGLE	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>50</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	SRP062493			10329X17_130822_SN141_0719_AD2CK5ACXX_6.txt.gz	fastq	1074686900.0	21493738.0	60hpf.hypoxia.2.10329X17	0:50	A:276432361;C:244039609;G:234638386;T:319556004;N:20540	50	276432361	244039609	234638386	319556004	20540	SRX1321815	SRS1109648	SRA289450	University of Utah\|Bonkowsky	University of Utah	1	0.9502	0.3077	0.6899	0.50645	50	B	usable mapping rate	illumina	hiseq_era	unknown	random_priming	trueseq	bulk	unknown	unknown	United States	2015-09-03	Hatching	Embryo	Brain	Nervous System
39846	39846	SRR2239869	SRX1321814	SRS1109646	SRP062493	PRJNA293106	Danio rerio Raw sequence reads	PRJNA293106	Metagenomics	Zebrafish Hypoxia RNAseq		RNAseq at 48hpf following hypoxia		48hpf hypoxia		strain:TU\|age:48hpf\|sex:not determined\|tissue:brain\|treatment:hypoxia\|BioSampleModel:Model organism or animal	Zebrafish Embryonic Development Hypoxia Versus Normoxia RNAseq: Sample 48hpf hypoxia	48hpf.hypoxia.2.10329X15	48hpf.hypoxia.2.10329X15	We collected whole RNA from zebrafish at five different embryonic developmental stages with experimental triplicates for each stage time point and experimental condition. For hypoxia we placed developing embryos in pre conditioned media in a sealed plexiglass chamber set to a reduced oxygen concentration for 12 hour periods. Immediately following hypoxia or normoxia embryos were sacrificed and RNA collected. We used 1% pO2 for ages up to 48 hpf and 4% pO2 for older ages. At the time point for collection embryos were dissected and only head tissue was used for subsequent RNA preparation. RNA was prepared with a miRNeasy kit Qiagen. Quality of total RNAs was checked using a NanoDrop 2000 Spectrometer and an Agilent 2100 Bioanalyzer. The sequencing libraries were created using the Illumina TruSeq Stranded Total RNA Sample Preparation Kit with Ribo Zero. These libraries were sequenced on an Illumina HiSeq2000 sequencer for 50 cycles ie. 50 nucleotides single end stranded using Illumina’s version 3 chemistry. 6 samples were multiplexed together in each lane of the sequencer providing an average yield of 25.1 million reads per sample. The base calling was performed using Illumina’s CASAVA version 1.8.2 software.			RNA-Seq	TRANSCRIPTOMIC	RANDOM	SINGLE	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>50</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	SRP062493			10329X15_130822_SN141_0719_AD2CK5ACXX_6.txt.gz	fastq	1111913550.0	22238271.0	48hpf.hypoxia.2.10329X15	0:50	A:288023952;C:248947169;G:243195362;T:331723738;N:23329	50	288023952	248947169	243195362	331723738	23329	SRX1321814	SRS1109646	SRA289450	University of Utah\|Bonkowsky	University of Utah	1	0.93753	0.34521	0.70246	0.51535	50	B	usable mapping rate	illumina	hiseq_era	unknown	random_priming	trueseq	bulk	unknown	unknown	United States	2015-10-10	Hatching	Embryo	Brain	Nervous System
39847	39847	SRR2239772	SRX1321813	SRS1109650	SRP062493	PRJNA293106	Danio rerio Raw sequence reads	PRJNA293106	Metagenomics	Zebrafish Hypoxia RNAseq		RNAseq at 36hpf following normoxia		36hpf normoxia		strain:TU\|age:36hpf\|sex:not determined\|tissue:brain\|treatment:normoxia\|BioSampleModel:Model organism or animal	Zebrafish Embryonic Development Hypoxia Versus Normoxia RNAseq: Sample 36hpf normoxia	36hpf.normoxia.2.10329X14	36hpf.normoxia.2.10329X14	We collected whole RNA from zebrafish at five different embryonic developmental stages with experimental triplicates for each stage time point and experimental condition. For hypoxia we placed developing embryos in pre conditioned media in a sealed plexiglass chamber set to a reduced oxygen concentration for 12 hour periods. Immediately following hypoxia or normoxia embryos were sacrificed and RNA collected. We used 1% pO2 for ages up to 48 hpf and 4% pO2 for older ages. At the time point for collection embryos were dissected and only head tissue was used for subsequent RNA preparation. RNA was prepared with a miRNeasy kit Qiagen. Quality of total RNAs was checked using a NanoDrop 2000 Spectrometer and an Agilent 2100 Bioanalyzer. The sequencing libraries were created using the Illumina TruSeq Stranded Total RNA Sample Preparation Kit with Ribo Zero. These libraries were sequenced on an Illumina HiSeq2000 sequencer for 50 cycles ie. 50 nucleotides single end stranded using Illumina’s version 3 chemistry. 6 samples were multiplexed together in each lane of the sequencer providing an average yield of 25.1 million reads per sample. The base calling was performed using Illumina’s CASAVA version 1.8.2 software.			RNA-Seq	TRANSCRIPTOMIC	RANDOM	SINGLE	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>50</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	SRP062493			10329X14_130822_SN141_0719_AD2CK5ACXX_6.txt.gz	fastq	1533885200.0	30677704.0	36hpf.normoxia.2.10329X14	0:50	A:392813056;C:354469675;G:348871622;T:437692730;N:38117	50	392813056	354469675	348871622	437692730	38117	SRX1321813	SRS1109650	SRA289450	University of Utah\|Bonkowsky	University of Utah	1	0.91439	0.31812	0.70863	0.51667	50	B	usable mapping rate	illumina	hiseq_era	unknown	random_priming	trueseq	bulk	unknown	unknown	United States	2015-10-10	Pharyngula	Embryo	Brain	Nervous System
39848	39848	SRR2232685	SRX1321812	SRS1109645	SRP062493	PRJNA293106	Danio rerio Raw sequence reads	PRJNA293106	Metagenomics	Zebrafish Hypoxia RNAseq		RNAseq at 36hpf following hypoxia		36hpf hypoxia		strain:TU\|age:36hpf\|sex:not determined\|tissue:brain\|treatment:hypoxia\|BioSampleModel:Model organism or animal	Zebrafish Embryonic Development Hypoxia Versus Normoxia RNAseq: Sample 36hpf hypoxia	36hpf.hypoxia.2.10329X13	36hpf.hypoxia.2.10329X13	We collected whole RNA from zebrafish at five different embryonic developmental stages with experimental triplicates for each stage time point and experimental condition. For hypoxia we placed developing embryos in pre conditioned media in a sealed plexiglass chamber set to a reduced oxygen concentration for 12 hour periods. Immediately following hypoxia or normoxia embryos were sacrificed and RNA collected. We used 1% pO2 for ages up to 48 hpf and 4% pO2 for older ages. At the time point for collection embryos were dissected and only head tissue was used for subsequent RNA preparation. RNA was prepared with a miRNeasy kit Qiagen. Quality of total RNAs was checked using a NanoDrop 2000 Spectrometer and an Agilent 2100 Bioanalyzer. The sequencing libraries were created using the Illumina TruSeq Stranded Total RNA Sample Preparation Kit with Ribo Zero. These libraries were sequenced on an Illumina HiSeq2000 sequencer for 50 cycles ie. 50 nucleotides single end stranded using Illumina’s version 3 chemistry. 6 samples were multiplexed together in each lane of the sequencer providing an average yield of 25.1 million reads per sample. The base calling was performed using Illumina’s CASAVA version 1.8.2 software.			RNA-Seq	TRANSCRIPTOMIC	RANDOM	SINGLE	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>50</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	SRP062493			10329X13_130822_SN141_0719_AD2CK5ACXX_6.txt.gz	fastq	1112846200.0	22256924.0	36hpf.hypoxia.2.10329X13	0:50	A:279147206;C:259582122;G:249642505;T:324452965;N:21402	50	279147206	259582122	249642505	324452965	21402	SRX1321812	SRS1109645	SRA289450	University of Utah\|Bonkowsky	University of Utah	1	0.94469	0.25952	0.72243	0.52701	50	B	usable mapping rate	illumina	hiseq_era	unknown	random_priming	trueseq	bulk	unknown	unknown	United States	2015-10-10	Pharyngula	Embryo	Brain	Nervous System
39849	39849	SRR2227658	SRX1321811	SRS1109651	SRP062493	PRJNA293106	Danio rerio Raw sequence reads	PRJNA293106	Metagenomics	Zebrafish Hypoxia RNAseq		RNAseq at 24hpf following normoxia		24hpf normoxia		strain:TU\|age:24hpf\|sex:not determined\|tissue:brain\|treatment:normoxia\|BioSampleModel:Model organism or animal	Zebrafish Embryonic Development Hypoxia Versus Normoxia RNAseq: Sample 24hpf normoxia	24hpf.normoxia.2.10329X12	24hpf.normoxia.2.10329X12	We collected whole RNA from zebrafish at five different embryonic developmental stages with experimental triplicates for each stage time point and experimental condition. For hypoxia we placed developing embryos in pre conditioned media in a sealed plexiglass chamber set to a reduced oxygen concentration for 12 hour periods. Immediately following hypoxia or normoxia embryos were sacrificed and RNA collected. We used 1% pO2 for ages up to 48 hpf and 4% pO2 for older ages. At the time point for collection embryos were dissected and only head tissue was used for subsequent RNA preparation. RNA was prepared with a miRNeasy kit Qiagen. Quality of total RNAs was checked using a NanoDrop 2000 Spectrometer and an Agilent 2100 Bioanalyzer. The sequencing libraries were created using the Illumina TruSeq Stranded Total RNA Sample Preparation Kit with Ribo Zero. These libraries were sequenced on an Illumina HiSeq2000 sequencer for 50 cycles ie. 50 nucleotides single end stranded using Illumina’s version 3 chemistry. 6 samples were multiplexed together in each lane of the sequencer providing an average yield of 25.1 million reads per sample. The base calling was performed using Illumina’s CASAVA version 1.8.2 software.			RNA-Seq	TRANSCRIPTOMIC	RANDOM	SINGLE	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>50</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	SRP062493			10329X12_130822_SN141_0719_AD2CK5ACXX_5.txt.gz	fastq	1243623750.0	24872475.0	24hpf.normoxia.2.10329X12	0:50	A:317179221;C:285081899;G:274648790;T:366643275;N:70565	50	317179221	285081899	274648790	366643275	70565	SRX1321811	SRS1109651	SRA289450	University of Utah\|Bonkowsky	University of Utah	1	0.95045	0.27525	0.69688	0.53555	50	B	usable mapping rate	illumina	hiseq_era	unknown	random_priming	trueseq	bulk	unknown	unknown	United States	2015-09-01	Pharyngula	Embryo	Brain	Nervous System
39850	39850	SRR2227378	SRX1321810	SRS1109647	SRP062493	PRJNA293106	Danio rerio Raw sequence reads	PRJNA293106	Metagenomics	Zebrafish Hypoxia RNAseq		RNAseq at 72hpf following normoxia		72hpf normoxia		strain:TU\|age:72hpf\|sex:not determined\|tissue:brain\|treatment:normoxia\|BioSampleModel:Model organism or animal	Zebrafish Embryonic Development Hypoxia Versus Normoxia RNAseq: Sample 72hpf normoxia	72hpf.normoxia.1.10329X10	72hpf.normoxia.1.10329X10	We collected whole RNA from zebrafish at five different embryonic developmental stages with experimental triplicates for each stage time point and experimental condition. For hypoxia we placed developing embryos in pre conditioned media in a sealed plexiglass chamber set to a reduced oxygen concentration for 12 hour periods. Immediately following hypoxia or normoxia embryos were sacrificed and RNA collected. We used 1% pO2 for ages up to 48 hpf and 4% pO2 for older ages. At the time point for collection embryos were dissected and only head tissue was used for subsequent RNA preparation. RNA was prepared with a miRNeasy kit Qiagen. Quality of total RNAs was checked using a NanoDrop 2000 Spectrometer and an Agilent 2100 Bioanalyzer. The sequencing libraries were created using the Illumina TruSeq Stranded Total RNA Sample Preparation Kit with Ribo Zero. These libraries were sequenced on an Illumina HiSeq2000 sequencer for 50 cycles ie. 50 nucleotides single end stranded using Illumina’s version 3 chemistry. 6 samples were multiplexed together in each lane of the sequencer providing an average yield of 25.1 million reads per sample. The base calling was performed using Illumina’s CASAVA version 1.8.2 software.			RNA-Seq	TRANSCRIPTOMIC	RANDOM	SINGLE	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>50</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	SRP062493			10329X10_130822_SN141_0719_AD2CK5ACXX_5.txt.gz	fastq	1268552500.0	25371050.0	72hpf.normoxia.1.10329X10	0:50	A:334230065;C:286785060;G:271795849;T:375668734;N:72792	50	334230065	286785060	271795849	375668734	72792	SRX1321810	SRS1109647	SRA289450	University of Utah\|Bonkowsky	University of Utah	1	0.94017	0.34471	0.67109	0.5244	50	B	usable mapping rate	illumina	hiseq_era	unknown	random_priming	trueseq	bulk	unknown	unknown	United States	2015-09-18	Larval	Larval	Brain	Nervous System
39851	39851	SRR2226742	SRX1321809	SRS1109649	SRP062493	PRJNA293106	Danio rerio Raw sequence reads	PRJNA293106	Metagenomics	Zebrafish Hypoxia RNAseq		RNAseq at 72hpf following hypoxia		72hpf hypoxia		strain:TU\|age:72hpf\|sex:not determined\|tissue:brain\|treatment:hypoxia\|BioSampleModel:Model organism or animal	Zebrafish Embryonic Development Hypoxia Versus Normoxia RNAseq: Sample 72hpf hypoxia	72hpf.hypoxia.1.10329X9	72hpf.hypoxia.1.10329X9	We collected whole RNA from zebrafish at five different embryonic developmental stages with experimental triplicates for each stage time point and experimental condition. For hypoxia we placed developing embryos in pre conditioned media in a sealed plexiglass chamber set to a reduced oxygen concentration for 12 hour periods. Immediately following hypoxia or normoxia embryos were sacrificed and RNA collected. We used 1% pO2 for ages up to 48 hpf and 4% pO2 for older ages. At the time point for collection embryos were dissected and only head tissue was used for subsequent RNA preparation. RNA was prepared with a miRNeasy kit Qiagen. Quality of total RNAs was checked using a NanoDrop 2000 Spectrometer and an Agilent 2100 Bioanalyzer. The sequencing libraries were created using the Illumina TruSeq Stranded Total RNA Sample Preparation Kit with Ribo Zero. These libraries were sequenced on an Illumina HiSeq2000 sequencer for 50 cycles ie. 50 nucleotides single end stranded using Illumina’s version 3 chemistry. 6 samples were multiplexed together in each lane of the sequencer providing an average yield of 25.1 million reads per sample. The base calling was performed using Illumina’s CASAVA version 1.8.2 software.			RNA-Seq	TRANSCRIPTOMIC	RANDOM	SINGLE	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>50</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	SRP062493			10329X9_130822_SN141_0719_AD2CK5ACXX_5.txt.gz	fastq	1625518100.0	32510362.0	72hpf.hypoxia.1.10329X9	0:50	A:428393257;C:366720934;G:349089971;T:481221434;N:92504	50	428393257	366720934	349089971	481221434	92504	SRX1321809	SRS1109649	SRA289450	University of Utah\|Bonkowsky	University of Utah	1	0.9444	0.36232	0.67503	0.53278	50	B	usable mapping rate	illumina	hiseq_era	unknown	random_priming	trueseq	bulk	unknown	unknown	United States	2015-10-10	Larval	Larval	Brain	Nervous System
39852	39852	SRR2225888	SRX1321808	SRS1109648	SRP062493	PRJNA293106	Danio rerio Raw sequence reads	PRJNA293106	Metagenomics	Zebrafish Hypoxia RNAseq		RNAseq at 60hpf following hypoxia		60hpf hypoxia		strain:TU\|age:60hpf\|sex:not determined\|tissue:brain\|treatment:hypoxia\|BioSampleModel:Model organism or animal	Zebrafish Embryonic Development Hypoxia Versus Normoxia RNAseq: Sample 60hpf hypoxia	60hpf.hypoxia.1.10329X7	60hpf.hypoxia.1.10329X7	We collected whole RNA from zebrafish at five different embryonic developmental stages with experimental triplicates for each stage time point and experimental condition. For hypoxia we placed developing embryos in pre conditioned media in a sealed plexiglass chamber set to a reduced oxygen concentration for 12 hour periods. Immediately following hypoxia or normoxia embryos were sacrificed and RNA collected. We used 1% pO2 for ages up to 48 hpf and 4% pO2 for older ages. At the time point for collection embryos were dissected and only head tissue was used for subsequent RNA preparation. RNA was prepared with a miRNeasy kit Qiagen. Quality of total RNAs was checked using a NanoDrop 2000 Spectrometer and an Agilent 2100 Bioanalyzer. The sequencing libraries were created using the Illumina TruSeq Stranded Total RNA Sample Preparation Kit with Ribo Zero. These libraries were sequenced on an Illumina HiSeq2000 sequencer for 50 cycles ie. 50 nucleotides single end stranded using Illumina’s version 3 chemistry. 6 samples were multiplexed together in each lane of the sequencer providing an average yield of 25.1 million reads per sample. The base calling was performed using Illumina’s CASAVA version 1.8.2 software.			RNA-Seq	TRANSCRIPTOMIC	RANDOM	SINGLE	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>50</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	SRP062493			10329X7_130822_SN141_0719_AD2CK5ACXX_5.txt.gz	fastq	1102232350.0	22044647.0	60hpf.hypoxia.1.10329X7	0:50	A:288570352;C:247978180;G:238128703;T:327492563;N:62552	50	288570352	247978180	238128703	327492563	62552	SRX1321808	SRS1109648	SRA289450	University of Utah\|Bonkowsky	University of Utah	1	0.94824	0.31808	0.69244	0.51505	50	B	usable mapping rate	illumina	hiseq_era	unknown	random_priming	trueseq	bulk	unknown	unknown	United States	2015-10-10	Hatching	Embryo	Brain	Nervous System
39853	39853	SRR2225789	SRX1321807	SRS1109646	SRP062493	PRJNA293106	Danio rerio Raw sequence reads	PRJNA293106	Metagenomics	Zebrafish Hypoxia RNAseq		RNAseq at 48hpf following hypoxia		48hpf hypoxia		strain:TU\|age:48hpf\|sex:not determined\|tissue:brain\|treatment:hypoxia\|BioSampleModel:Model organism or animal	Zebrafish Embryonic Development Hypoxia Versus Normoxia RNAseq: Sample 48hpf hypoxia	48hpf.hypoxia.1.10329X6	48hpf.hypoxia.1.10329X6	We collected whole RNA from zebrafish at five different embryonic developmental stages with experimental triplicates for each stage time point and experimental condition. For hypoxia we placed developing embryos in pre conditioned media in a sealed plexiglass chamber set to a reduced oxygen concentration for 12 hour periods. Immediately following hypoxia or normoxia embryos were sacrificed and RNA collected. We used 1% pO2 for ages up to 48 hpf and 4% pO2 for older ages. At the time point for collection embryos were dissected and only head tissue was used for subsequent RNA preparation. RNA was prepared with a miRNeasy kit Qiagen. Quality of total RNAs was checked using a NanoDrop 2000 Spectrometer and an Agilent 2100 Bioanalyzer. The sequencing libraries were created using the Illumina TruSeq Stranded Total RNA Sample Preparation Kit with Ribo Zero. These libraries were sequenced on an Illumina HiSeq2000 sequencer for 50 cycles ie. 50 nucleotides single end stranded using Illumina’s version 3 chemistry. 6 samples were multiplexed together in each lane of the sequencer providing an average yield of 25.1 million reads per sample. The base calling was performed using Illumina’s CASAVA version 1.8.2 software.			RNA-Seq	TRANSCRIPTOMIC	RANDOM	SINGLE	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>50</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	SRP062493			10329X6_130822_SN141_0719_AD2CK5ACXX_4.txt.gz	fastq	1250042950.0	25000859.0	48hpf.hypoxia.1.10329X6	0:50	A:312511165;C:283310381;G:277279378;T:376891744;N:50282	50	312511165	283310381	277279378	376891744	50282	SRX1321807	SRS1109646	SRA289450	University of Utah\|Bonkowsky	University of Utah	1	0.94959	0.28331	0.68757	0.53976	50	B	usable mapping rate	illumina	hiseq_era	unknown	random_priming	trueseq	bulk	unknown	unknown	United States	2015-09-01	Hatching	Embryo	Brain	Nervous System
39854	39854	SRR2225675	SRX1321806	SRS1109650	SRP062493	PRJNA293106	Danio rerio Raw sequence reads	PRJNA293106	Metagenomics	Zebrafish Hypoxia RNAseq		RNAseq at 36hpf following normoxia		36hpf normoxia		strain:TU\|age:36hpf\|sex:not determined\|tissue:brain\|treatment:normoxia\|BioSampleModel:Model organism or animal	Zebrafish Embryonic Development Hypoxia Versus Normoxia RNAseq: Sample 36hpf normoxia	36hpf.normoxia.1.10329X5	36hpf.normoxia.1.10329X5	We collected whole RNA from zebrafish at five different embryonic developmental stages with experimental triplicates for each stage time point and experimental condition. For hypoxia we placed developing embryos in pre conditioned media in a sealed plexiglass chamber set to a reduced oxygen concentration for 12 hour periods. Immediately following hypoxia or normoxia embryos were sacrificed and RNA collected. We used 1% pO2 for ages up to 48 hpf and 4% pO2 for older ages. At the time point for collection embryos were dissected and only head tissue was used for subsequent RNA preparation. RNA was prepared with a miRNeasy kit Qiagen. Quality of total RNAs was checked using a NanoDrop 2000 Spectrometer and an Agilent 2100 Bioanalyzer. The sequencing libraries were created using the Illumina TruSeq Stranded Total RNA Sample Preparation Kit with Ribo Zero. These libraries were sequenced on an Illumina HiSeq2000 sequencer for 50 cycles ie. 50 nucleotides single end stranded using Illumina’s version 3 chemistry. 6 samples were multiplexed together in each lane of the sequencer providing an average yield of 25.1 million reads per sample. The base calling was performed using Illumina’s CASAVA version 1.8.2 software.			RNA-Seq	TRANSCRIPTOMIC	RANDOM	SINGLE	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>50</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	SRP062493			10329X5_130822_SN141_0719_AD2CK5ACXX_4.txt.gz	fastq	1244744700.0	24894894.0	36hpf.normoxia.1.10329X5	0:50	A:301597123;C:287750990;G:281194881;T:374152417;N:49289	50	301597123	287750990	281194881	374152417	49289	SRX1321806	SRS1109650	SRA289450	University of Utah\|Bonkowsky	University of Utah	1	0.95571	0.22791	0.71177	0.53801	50	B	usable mapping rate	illumina	hiseq_era	unknown	random_priming	trueseq	bulk	unknown	unknown	United States	2015-10-10	Pharyngula	Embryo	Brain	Nervous System
39855	39855	SRR2225572	SRX1321805	SRS1109651	SRP062493	PRJNA293106	Danio rerio Raw sequence reads	PRJNA293106	Metagenomics	Zebrafish Hypoxia RNAseq		RNAseq at 24hpf following normoxia		24hpf normoxia		strain:TU\|age:24hpf\|sex:not determined\|tissue:brain\|treatment:normoxia\|BioSampleModel:Model organism or animal	Zebrafish Embryonic Development Hypoxia Versus Normoxia RNAseq: Sample 24hpf normoxia	24hpf.normoxia.1.10329X3	24hpf.normoxia.1.10329X3	We collected whole RNA from zebrafish at five different embryonic developmental stages with experimental triplicates for each stage time point and experimental condition. For hypoxia we placed developing embryos in pre conditioned media in a sealed plexiglass chamber set to a reduced oxygen concentration for 12 hour periods. Immediately following hypoxia or normoxia embryos were sacrificed and RNA collected. We used 1% pO2 for ages up to 48 hpf and 4% pO2 for older ages. At the time point for collection embryos were dissected and only head tissue was used for subsequent RNA preparation. RNA was prepared with a miRNeasy kit Qiagen. Quality of total RNAs was checked using a NanoDrop 2000 Spectrometer and an Agilent 2100 Bioanalyzer. The sequencing libraries were created using the Illumina TruSeq Stranded Total RNA Sample Preparation Kit with Ribo Zero. These libraries were sequenced on an Illumina HiSeq2000 sequencer for 50 cycles ie. 50 nucleotides single end stranded using Illumina’s version 3 chemistry. 6 samples were multiplexed together in each lane of the sequencer providing an average yield of 25.1 million reads per sample. The base calling was performed using Illumina’s CASAVA version 1.8.2 software.			RNA-Seq	TRANSCRIPTOMIC	RANDOM	SINGLE	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>50</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	SRP062493			10329X3_130822_SN141_0719_AD2CK5ACXX_4.txt.gz	fastq	1293605500.0	25872110.0	24hpf.normoxia.1.10329X3	0:50	A:310265067;C:296406990;G:295054246;T:391826687;N:52510	50	310265067	296406990	295054246	391826687	52510	SRX1321805	SRS1109651	SRA289450	University of Utah\|Bonkowsky	University of Utah	1	0.95596	0.21907	0.71512	0.39029	50	B	usable mapping rate	illumina	hiseq_era	unknown	random_priming	trueseq	bulk	unknown	unknown	United States	2015-09-01	Pharyngula	Embryo	Brain	Nervous System
39856	39856	SRR2222835	SRX1321804	SRS1109643	SRP062493	PRJNA293106	Danio rerio Raw sequence reads	PRJNA293106	Metagenomics	Zebrafish Hypoxia RNAseq		RNAseq at 48hpf following normoxia		48hpf normoxia		strain:TU\|age:48hpf\|sex:not determined\|tissue:brain\|treatment:normoxia\|BioSampleModel:Model organism or animal	Zebrafish Embryonic Development Hypoxia Versus Normoxia RNAseq: Sample 48hpf normoxia	48hpf.1.10329X1	48hpf.1.10329X1	We collected whole RNA from zebrafish at five different embryonic developmental stages with experimental triplicates for each stage time point and experimental condition. For hypoxia we placed developing embryos in pre conditioned media in a sealed plexiglass chamber set to a reduced oxygen concentration for 12 hour periods. Immediately following hypoxia or normoxia embryos were sacrificed and RNA collected. We used 1% pO2 for ages up to 48 hpf and 4% pO2 for older ages. At the time point for collection embryos were dissected and only head tissue was used for subsequent RNA preparation. RNA was prepared with a miRNeasy kit Qiagen. Quality of total RNAs was checked using a NanoDrop 2000 Spectrometer and an Agilent 2100 Bioanalyzer. The sequencing libraries were created using the Illumina TruSeq Stranded Total RNA Sample Preparation Kit with Ribo Zero. These libraries were sequenced on an Illumina HiSeq2000 sequencer for 50 cycles ie. 50 nucleotides single end stranded using Illumina’s version 3 chemistry. 6 samples were multiplexed together in each lane of the sequencer providing an average yield of 25.1 million reads per sample. The base calling was performed using Illumina’s CASAVA version 1.8.2 software.			RNA-Seq	TRANSCRIPTOMIC	RANDOM	SINGLE	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>50</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	SRP062493			10329X1_130822_SN141_0719_AD2CK5ACXX_4.txt.gz	fastq	1215136450.0	24302729.0	48hpf.1.10329X1	0:50	A:291802893;C:276442849;G:277217342;T:369624915;N:48451	50	291802893	276442849	277217342	369624915	48451	SRX1321804	SRS1109643	SRA289450	University of Utah\|Bonkowsky	University of Utah	1	0.95575	0.23021	0.72679	0.60296	50	B	usable mapping rate	illumina	hiseq_era	unknown	random_priming	trueseq	bulk	unknown	unknown	United States	2015-09-01	Hatching	Embryo	Brain	Nervous System

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CREATE TABLE run_metadata("run.accession" VARCHAR, "experiment.accession" VARCHAR, "sample.accession" VARCHAR, "study.accession" VARCHAR, bioproject VARCHAR, "study.title" VARCHAR, "study.alias" VARCHAR, "study.type" VARCHAR, "study.abstract" VARCHAR, "study.attributes" VARCHAR, "study.PMIDs" VARCHAR, "sample.description" VARCHAR, "sample.title" VARCHAR, "sample.alias" VARCHAR, "sample.centername" VARCHAR, "sample.attributes" VARCHAR, "GEOsample.title" VARCHAR, "GEOsample.dataprocessing" VARCHAR, "GEOsample.source" VARCHAR, "GEOsample.treatmentprotocol" VARCHAR, "GEOsample.extractprotocol" VARCHAR, "GEOsample.growthprotocol" VARCHAR, "GEOsample.characteristics" VARCHAR, "GEOsample.accession" VARCHAR, "experiment.title" VARCHAR, "experiment.alias" VARCHAR, "experiment.library_name" VARCHAR, "experiment.design_description" VARCHAR, "experiment.library_construction_protocol" VARCHAR, "experiment.attributes" VARCHAR, "experiment.library_strategy" VARCHAR, "experiment.library_source" VARCHAR, "experiment.library_selection" VARCHAR, "experiment.library_layout" VARCHAR, "experiment.platform" VARCHAR, "experiment.instrument_model" VARCHAR, "experiment.spot_descriptor" VARCHAR, "experiment.study_ref" VARCHAR, "run.title" VARCHAR, "run.attributes" VARCHAR, "run.filename" VARCHAR, "run.semantic_name" VARCHAR, "run.total_bases" DOUBLE, "run.total_spots" DOUBLE, "run.alias" VARCHAR, "run.read_lengths" VARCHAR, "run.base_counts" VARCHAR, "run.r1_length" BIGINT, "run.r2_length" BIGINT, "run.r3_length" BIGINT, "run.r4_length" BIGINT, "run.Acount" BIGINT, "run.Ccount" BIGINT, "run.Gcount" BIGINT, "run.Tcount" BIGINT, "run.Ncount" BIGINT, "run.experiment" VARCHAR, "run.pool_member" VARCHAR, "submission.accession" VARCHAR, "submission.srasource" VARCHAR, "submission.bioprojectsource" VARCHAR, "seqdetective.n_mates" BIGINT, "seqdetective.mapping_rate.mate1" DOUBLE, "seqdetective.mapping_rate.mate2" DOUBLE, "seqdetective.nofeature_rate.mate1" DOUBLE, "seqdetective.nofeature_rate.mate2" DOUBLE, "seqdetective.sparsity.mate1" DOUBLE, "seqdetective.sparsity.mate2" DOUBLE, "seqdetective.pos_strand_rate.mate1" DOUBLE, "seqdetective.pos_strand_rate.mate2" DOUBLE, "seqdetective.readlen.mate1" BIGINT, "seqdetective.readlen.mate2" BIGINT, "seqdetective.judgement.mate1" VARCHAR, "seqdetective.judgement.mate2" VARCHAR, "seqdetective.judgement.reason" VARCHAR, platform_family VARCHAR, instrument_generation VARCHAR, read_bias VARCHAR, selection_class VARCHAR, prep_kit VARCHAR, sc_or_bulk VARCHAR, tech_class VARCHAR, technology VARCHAR, tech_variant VARCHAR, "submission.bioprojectsource.country" VARCHAR, earliest_date DATE, devstage_curation VARCHAR, devstage_curation_coarse VARCHAR, tissue_curation VARCHAR, tissue_curation_coarse VARCHAR);;
CREATE INDEX idx_run_bioproject ON run_metadata(bioproject);;
CREATE INDEX idx_run_run_accession ON run_metadata("run.accession");;