run_metadata
44 rows where experiment.library_layout = "SINGLE", experiment.library_selection = "PolyA" and tissue_curation = "Head"
This data as json, CSV (advanced)
| Link | rowid ▼ | run.accession | experiment.accession | sample.accession | study.accession | bioproject | study.title | study.alias | study.type | study.abstract | study.attributes | study.PMIDs | sample.description | sample.title | sample.alias | sample.centername | sample.attributes | GEOsample.title | GEOsample.dataprocessing | GEOsample.source | GEOsample.treatmentprotocol | GEOsample.extractprotocol | GEOsample.growthprotocol | GEOsample.characteristics | GEOsample.accession | experiment.title | experiment.alias | experiment.library_name | experiment.design_description | experiment.library_construction_protocol | experiment.attributes | experiment.library_strategy | experiment.library_source | experiment.library_selection | experiment.library_layout | experiment.platform | experiment.instrument_model | experiment.spot_descriptor | experiment.study_ref | run.title | run.attributes | run.filename | run.semantic_name | run.total_bases | run.total_spots | run.alias | run.read_lengths | run.base_counts | run.r1_length | run.r2_length | run.r3_length | run.r4_length | run.Acount | run.Ccount | run.Gcount | run.Tcount | run.Ncount | run.experiment | run.pool_member | submission.accession | submission.srasource | submission.bioprojectsource | seqdetective.n_mates | seqdetective.mapping_rate.mate1 | seqdetective.mapping_rate.mate2 | seqdetective.nofeature_rate.mate1 | seqdetective.nofeature_rate.mate2 | seqdetective.sparsity.mate1 | seqdetective.sparsity.mate2 | seqdetective.pos_strand_rate.mate1 | seqdetective.pos_strand_rate.mate2 | seqdetective.readlen.mate1 | seqdetective.readlen.mate2 | seqdetective.judgement.mate1 | seqdetective.judgement.mate2 | seqdetective.judgement.reason | platform_family | instrument_generation | read_bias | selection_class | prep_kit | sc_or_bulk | tech_class | technology | tech_variant | submission.bioprojectsource.country | earliest_date | devstage_curation | devstage_curation_coarse | tissue_curation | tissue_curation_coarse |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 10407 | 10407 | ERR9077202 | ERX8620336 | ERS10857224 | ERP135966 | PRJEB51339 | RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | E-MTAB-11505 | Transcriptome Analysis | Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. | ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 | Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. | Sv2aWT5S8 | SAMEA13254181 | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg | ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254181|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aWT5S8|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/+|individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aWT5S8|sex:not available|strain:AB | NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | E MTAB 11505:Sv2aWT5S8 s | Sv2aWT5S8 s | RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. | Experimental Factor: genotype:sv2a+/+ | RNA-Seq | TRANSCRIPTOMIC | PolyA | SINGLE | ILLUMINA | NextSeq 500 | ERP135966 | NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 | sv2aWT5S8L001-1.fastq.gz | fastq | 652046291.0 | 8633105.0 | E MTAB 11505:sv2aWT5S8L001 | 0:75.53 1:0 | A:159570243;C:156420495;G:154853315;T:181073030;N:129208 | 75 | 0 | 159570243 | 156420495 | 154853315 | 181073030 | 129208 | ERX8620336 | ERS10857224 | ERA9644804 | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive | 1 | 0.95245 | 0.1148 | 0.65756 | 0.47011 | 75 | B | usable mapping rate | illumina | nextseq | unknown | poly_a | trueseq | bulk | unknown | unknown | Belgium | 2022-04-21 | Larval | Larval | Head | Nervous System | |||||||||||||||||||
| 10408 | 10408 | ERR9077203 | ERX8620336 | ERS10857224 | ERP135966 | PRJEB51339 | RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | E-MTAB-11505 | Transcriptome Analysis | Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. | ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 | Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. | Sv2aWT5S8 | SAMEA13254181 | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg | ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254181|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aWT5S8|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/+|individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aWT5S8|sex:not available|strain:AB | NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | E MTAB 11505:Sv2aWT5S8 s | Sv2aWT5S8 s | RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. | Experimental Factor: genotype:sv2a+/+ | RNA-Seq | TRANSCRIPTOMIC | PolyA | SINGLE | ILLUMINA | NextSeq 500 | ERP135966 | NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 | sv2aWT5S8L002-1.fastq.gz | fastq | 653840525.0 | 8656886.0 | E MTAB 11505:sv2aWT5S8L002 | 0:75.53 1:0 | A:159902458;C:156826842;G:155509070;T:181475104;N:127051 | 75 | 0 | 159902458 | 156826842 | 155509070 | 181475104 | 127051 | ERX8620336 | ERS10857224 | ERA9644804 | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive | 1 | 0.95226 | 0.1156 | 0.65792 | 0.4667 | 76 | B | usable mapping rate | illumina | nextseq | unknown | poly_a | trueseq | bulk | unknown | unknown | Belgium | 2022-04-21 | Larval | Larval | Head | Nervous System | |||||||||||||||||||
| 10409 | 10409 | ERR9077204 | ERX8620336 | ERS10857224 | ERP135966 | PRJEB51339 | RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | E-MTAB-11505 | Transcriptome Analysis | Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. | ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 | Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. | Sv2aWT5S8 | SAMEA13254181 | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg | ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254181|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aWT5S8|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/+|individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aWT5S8|sex:not available|strain:AB | NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | E MTAB 11505:Sv2aWT5S8 s | Sv2aWT5S8 s | RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. | Experimental Factor: genotype:sv2a+/+ | RNA-Seq | TRANSCRIPTOMIC | PolyA | SINGLE | ILLUMINA | NextSeq 500 | ERP135966 | NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 | sv2aWT5S8L003-1.fastq.gz | fastq | 659584196.0 | 8732885.0 | E MTAB 11505:sv2aWT5S8L003 | 0:75.53 1:0 | A:161257146;C:158299734;G:156793443;T:183128972;N:104901 | 75 | 0 | 161257146 | 158299734 | 156793443 | 183128972 | 104901 | ERX8620336 | ERS10857224 | ERA9644804 | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive | 1 | 0.95298 | 0.11485 | 0.65798 | 0.46853 | 76 | B | usable mapping rate | illumina | nextseq | unknown | poly_a | trueseq | bulk | unknown | unknown | Belgium | 2022-04-21 | Larval | Larval | Head | Nervous System | |||||||||||||||||||
| 10410 | 10410 | ERR9077205 | ERX8620336 | ERS10857224 | ERP135966 | PRJEB51339 | RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | E-MTAB-11505 | Transcriptome Analysis | Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. | ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 | Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. | Sv2aWT5S8 | SAMEA13254181 | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg | ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254181|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aWT5S8|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/+|individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aWT5S8|sex:not available|strain:AB | NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | E MTAB 11505:Sv2aWT5S8 s | Sv2aWT5S8 s | RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. | Experimental Factor: genotype:sv2a+/+ | RNA-Seq | TRANSCRIPTOMIC | PolyA | SINGLE | ILLUMINA | NextSeq 500 | ERP135966 | NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 | sv2aWT5S8L004-1.fastq.gz | fastq | 666627746.0 | 8826123.0 | E MTAB 11505:sv2aWT5S8L004 | 0:75.53 1:0 | A:162975131;C:159937258;G:158506242;T:185106149;N:102966 | 75 | 0 | 162975131 | 159937258 | 158506242 | 185106149 | 102966 | ERX8620336 | ERS10857224 | ERA9644804 | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive | 1 | 0.9531 | 0.11425 | 0.65736 | 0.47309 | 76 | B | usable mapping rate | illumina | nextseq | unknown | poly_a | trueseq | bulk | unknown | unknown | Belgium | 2022-04-21 | Larval | Larval | Head | Nervous System | |||||||||||||||||||
| 10411 | 10411 | ERR9077198 | ERX8620335 | ERS10857223 | ERP135966 | PRJEB51339 | RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | E-MTAB-11505 | Transcriptome Analysis | Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. | ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 | Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. | Sv2aWT4S7 | SAMEA13254180 | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg | ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254180|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aWT4S7|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/+|individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aWT4S7|sex:not available|strain:AB | NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | E MTAB 11505:Sv2aWT4S7 s | Sv2aWT4S7 s | RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. | Experimental Factor: genotype:sv2a+/+ | RNA-Seq | TRANSCRIPTOMIC | PolyA | SINGLE | ILLUMINA | NextSeq 500 | ERP135966 | NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 | sv2aWT4S7L001-1.fastq.gz | fastq | 683751258.0 | 9051795.0 | E MTAB 11505:sv2aWT4S7L001 | 0:75.54 1:0 | A:162889923;C:167127644;G:164779384;T:188822907;N:131400 | 75 | 0 | 162889923 | 167127644 | 164779384 | 188822907 | 131400 | ERX8620335 | ERS10857223 | ERA9644804 | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive | 1 | 0.95739 | 0.0959 | 0.6606 | 0.46826 | 75 | B | usable mapping rate | illumina | nextseq | unknown | poly_a | trueseq | bulk | unknown | unknown | Belgium | 2022-04-21 | Larval | Larval | Head | Nervous System | |||||||||||||||||||
| 10412 | 10412 | ERR9077199 | ERX8620335 | ERS10857223 | ERP135966 | PRJEB51339 | RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | E-MTAB-11505 | Transcriptome Analysis | Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. | ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 | Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. | Sv2aWT4S7 | SAMEA13254180 | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg | ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254180|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aWT4S7|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/+|individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aWT4S7|sex:not available|strain:AB | NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | E MTAB 11505:Sv2aWT4S7 s | Sv2aWT4S7 s | RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. | Experimental Factor: genotype:sv2a+/+ | RNA-Seq | TRANSCRIPTOMIC | PolyA | SINGLE | ILLUMINA | NextSeq 500 | ERP135966 | NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 | sv2aWT4S7L002-1.fastq.gz | fastq | 684187711.0 | 9057591.0 | E MTAB 11505:sv2aWT4S7L002 | 0:75.54 1:0 | A:162923879;C:167246213;G:165071145;T:188818774;N:127700 | 75 | 0 | 162923879 | 167246213 | 165071145 | 188818774 | 127700 | ERX8620335 | ERS10857223 | ERA9644804 | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive | 1 | 0.95724 | 0.0959 | 0.66036 | 0.47026 | 76 | B | usable mapping rate | illumina | nextseq | unknown | poly_a | trueseq | bulk | unknown | unknown | Belgium | 2022-04-21 | Larval | Larval | Head | Nervous System | |||||||||||||||||||
| 10413 | 10413 | ERR9077200 | ERX8620335 | ERS10857223 | ERP135966 | PRJEB51339 | RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | E-MTAB-11505 | Transcriptome Analysis | Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. | ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 | Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. | Sv2aWT4S7 | SAMEA13254180 | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg | ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254180|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aWT4S7|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/+|individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aWT4S7|sex:not available|strain:AB | NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | E MTAB 11505:Sv2aWT4S7 s | Sv2aWT4S7 s | RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. | Experimental Factor: genotype:sv2a+/+ | RNA-Seq | TRANSCRIPTOMIC | PolyA | SINGLE | ILLUMINA | NextSeq 500 | ERP135966 | NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 | sv2aWT4S7L003-1.fastq.gz | fastq | 692320213.0 | 9165132.0 | E MTAB 11505:sv2aWT4S7L003 | 0:75.54 1:0 | A:164772792;C:169329920;G:166952052;T:191164422;N:101027 | 75 | 0 | 164772792 | 169329920 | 166952052 | 191164422 | 101027 | ERX8620335 | ERS10857223 | ERA9644804 | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive | 1 | 0.95786 | 0.09701 | 0.66131 | 0.47564 | 76 | B | usable mapping rate | illumina | nextseq | unknown | poly_a | trueseq | bulk | unknown | unknown | Belgium | 2022-04-21 | Larval | Larval | Head | Nervous System | |||||||||||||||||||
| 10414 | 10414 | ERR9077201 | ERX8620335 | ERS10857223 | ERP135966 | PRJEB51339 | RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | E-MTAB-11505 | Transcriptome Analysis | Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. | ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 | Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. | Sv2aWT4S7 | SAMEA13254180 | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg | ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254180|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aWT4S7|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/+|individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aWT4S7|sex:not available|strain:AB | NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | E MTAB 11505:Sv2aWT4S7 s | Sv2aWT4S7 s | RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. | Experimental Factor: genotype:sv2a+/+ | RNA-Seq | TRANSCRIPTOMIC | PolyA | SINGLE | ILLUMINA | NextSeq 500 | ERP135966 | NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 | sv2aWT4S7L004-1.fastq.gz | fastq | 697047881.0 | 9227702.0 | E MTAB 11505:sv2aWT4S7L004 | 0:75.54 1:0 | A:165881728;C:170388393;G:168105900;T:192572068;N:99792 | 75 | 0 | 165881728 | 170388393 | 168105900 | 192572068 | 99792 | ERX8620335 | ERS10857223 | ERA9644804 | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive | 1 | 0.9584 | 0.09565 | 0.66115 | 0.46967 | 76 | B | usable mapping rate | illumina | nextseq | unknown | poly_a | trueseq | bulk | unknown | unknown | Belgium | 2022-04-21 | Larval | Larval | Head | Nervous System | |||||||||||||||||||
| 10415 | 10415 | ERR9077194 | ERX8620334 | ERS10857222 | ERP135966 | PRJEB51339 | RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | E-MTAB-11505 | Transcriptome Analysis | Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. | ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 | Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. | Sv2aWT3S2 | SAMEA13254179 | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg | ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254179|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aWT3S2|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/+|individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aWT3S2|sex:not available|strain:AB | NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | E MTAB 11505:Sv2aWT3S2 s | Sv2aWT3S2 s | RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. | Experimental Factor: genotype:sv2a+/+ | RNA-Seq | TRANSCRIPTOMIC | PolyA | SINGLE | ILLUMINA | NextSeq 500 | ERP135966 | NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 | sv2aWT3S2L001-1.fastq.gz | fastq | 723508205.0 | 9579193.0 | E MTAB 11505:sv2aWT3S2L001 | 0:75.53 1:0 | A:177832489;C:174193135;G:169978598;T:201360184;N:143799 | 75 | 0 | 177832489 | 174193135 | 169978598 | 201360184 | 143799 | ERX8620334 | ERS10857222 | ERA9644804 | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive | 1 | 0.95218 | 0.11732 | 0.65916 | 0.47955 | 71 | B | usable mapping rate | illumina | nextseq | unknown | poly_a | trueseq | bulk | unknown | unknown | Belgium | 2022-04-21 | Larval | Larval | Head | Nervous System | |||||||||||||||||||
| 10416 | 10416 | ERR9077195 | ERX8620334 | ERS10857222 | ERP135966 | PRJEB51339 | RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | E-MTAB-11505 | Transcriptome Analysis | Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. | ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 | Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. | Sv2aWT3S2 | SAMEA13254179 | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg | ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254179|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aWT3S2|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/+|individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aWT3S2|sex:not available|strain:AB | NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | E MTAB 11505:Sv2aWT3S2 s | Sv2aWT3S2 s | RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. | Experimental Factor: genotype:sv2a+/+ | RNA-Seq | TRANSCRIPTOMIC | PolyA | SINGLE | ILLUMINA | NextSeq 500 | ERP135966 | NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 | sv2aWT3S2L002-1.fastq.gz | fastq | 726190024.0 | 9614674.0 | E MTAB 11505:sv2aWT3S2L002 | 0:75.53 1:0 | A:178408224;C:174776263;G:170820745;T:202047224;N:137568 | 75 | 0 | 178408224 | 174776263 | 170820745 | 202047224 | 137568 | ERX8620334 | ERS10857222 | ERA9644804 | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive | 1 | 0.95093 | 0.11567 | 0.65865 | 0.46877 | 75 | B | usable mapping rate | illumina | nextseq | unknown | poly_a | trueseq | bulk | unknown | unknown | Belgium | 2022-04-21 | Larval | Larval | Head | Nervous System | |||||||||||||||||||
| 10417 | 10417 | ERR9077196 | ERX8620334 | ERS10857222 | ERP135966 | PRJEB51339 | RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | E-MTAB-11505 | Transcriptome Analysis | Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. | ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 | Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. | Sv2aWT3S2 | SAMEA13254179 | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg | ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254179|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aWT3S2|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/+|individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aWT3S2|sex:not available|strain:AB | NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | E MTAB 11505:Sv2aWT3S2 s | Sv2aWT3S2 s | RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. | Experimental Factor: genotype:sv2a+/+ | RNA-Seq | TRANSCRIPTOMIC | PolyA | SINGLE | ILLUMINA | NextSeq 500 | ERP135966 | NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 | sv2aWT3S2L003-1.fastq.gz | fastq | 735499955.0 | 9737845.0 | E MTAB 11505:sv2aWT3S2L003 | 0:75.53 1:0 | A:180659962;C:177141362;G:172905323;T:204678308;N:115000 | 75 | 0 | 180659962 | 177141362 | 172905323 | 204678308 | 115000 | ERX8620334 | ERS10857222 | ERA9644804 | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive | 1 | 0.95233 | 0.11614 | 0.65898 | 0.47114 | 76 | B | usable mapping rate | illumina | nextseq | unknown | poly_a | trueseq | bulk | unknown | unknown | Belgium | 2022-04-21 | Larval | Larval | Head | Nervous System | |||||||||||||||||||
| 10418 | 10418 | ERR9077197 | ERX8620334 | ERS10857222 | ERP135966 | PRJEB51339 | RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | E-MTAB-11505 | Transcriptome Analysis | Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. | ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 | Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. | Sv2aWT3S2 | SAMEA13254179 | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg | ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254179|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aWT3S2|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/+|individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aWT3S2|sex:not available|strain:AB | NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | E MTAB 11505:Sv2aWT3S2 s | Sv2aWT3S2 s | RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. | Experimental Factor: genotype:sv2a+/+ | RNA-Seq | TRANSCRIPTOMIC | PolyA | SINGLE | ILLUMINA | NextSeq 500 | ERP135966 | NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 | sv2aWT3S2L004-1.fastq.gz | fastq | 745285512.0 | 9867337.0 | E MTAB 11505:sv2aWT3S2L004 | 0:75.53 1:0 | A:183067487;C:179408382;G:175250814;T:207445912;N:112917 | 75 | 0 | 183067487 | 179408382 | 175250814 | 207445912 | 112917 | ERX8620334 | ERS10857222 | ERA9644804 | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive | 1 | 0.95245 | 0.11655 | 0.65857 | 0.47087 | 76 | B | usable mapping rate | illumina | nextseq | unknown | poly_a | trueseq | bulk | unknown | unknown | Belgium | 2022-04-21 | Larval | Larval | Head | Nervous System | |||||||||||||||||||
| 10419 | 10419 | ERR9077190 | ERX8620333 | ERS10857221 | ERP135966 | PRJEB51339 | RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | E-MTAB-11505 | Transcriptome Analysis | Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. | ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 | Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. | Sv2aWT1S1 | SAMEA13254178 | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg | ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254178|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aWT1S1|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/+|individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aWT1S1|sex:not available|strain:AB | NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | E MTAB 11505:Sv2aWT1S1 s | Sv2aWT1S1 s | RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. | Experimental Factor: genotype:sv2a+/+ | RNA-Seq | TRANSCRIPTOMIC | PolyA | SINGLE | ILLUMINA | NextSeq 500 | ERP135966 | NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 | sv2aWT1S1L001-1.fastq.gz | fastq | 647414748.0 | 8571883.0 | E MTAB 11505:sv2aWT1S1L001 | 0:75.53 1:0 | A:154593859;C:160498057;G:153809612;T:178389112;N:124108 | 75 | 0 | 154593859 | 160498057 | 153809612 | 178389112 | 124108 | ERX8620333 | ERS10857221 | ERA9644804 | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive | 1 | 0.95955 | 0.08686 | 0.65955 | 0.47406 | 76 | B | usable mapping rate | illumina | nextseq | unknown | poly_a | trueseq | bulk | unknown | unknown | Belgium | 2022-04-21 | Larval | Larval | Head | Nervous System | |||||||||||||||||||
| 10420 | 10420 | ERR9077191 | ERX8620333 | ERS10857221 | ERP135966 | PRJEB51339 | RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | E-MTAB-11505 | Transcriptome Analysis | Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. | ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 | Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. | Sv2aWT1S1 | SAMEA13254178 | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg | ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254178|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aWT1S1|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/+|individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aWT1S1|sex:not available|strain:AB | NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | E MTAB 11505:Sv2aWT1S1 s | Sv2aWT1S1 s | RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. | Experimental Factor: genotype:sv2a+/+ | RNA-Seq | TRANSCRIPTOMIC | PolyA | SINGLE | ILLUMINA | NextSeq 500 | ERP135966 | NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 | sv2aWT1S1L002-1.fastq.gz | fastq | 646657758.0 | 8561861.0 | E MTAB 11505:sv2aWT1S1L002 | 0:75.53 1:0 | A:154348044;C:160294625;G:153804048;T:178092212;N:118829 | 75 | 0 | 154348044 | 160294625 | 153804048 | 178092212 | 118829 | ERX8620333 | ERS10857221 | ERA9644804 | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive | 1 | 0.95942 | 0.08632 | 0.65991 | 0.47539 | 76 | B | usable mapping rate | illumina | nextseq | unknown | poly_a | trueseq | bulk | unknown | unknown | Belgium | 2022-04-21 | Larval | Larval | Head | Nervous System | |||||||||||||||||||
| 10421 | 10421 | ERR9077192 | ERX8620333 | ERS10857221 | ERP135966 | PRJEB51339 | RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | E-MTAB-11505 | Transcriptome Analysis | Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. | ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 | Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. | Sv2aWT1S1 | SAMEA13254178 | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg | ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254178|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aWT1S1|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/+|individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aWT1S1|sex:not available|strain:AB | NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | E MTAB 11505:Sv2aWT1S1 s | Sv2aWT1S1 s | RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. | Experimental Factor: genotype:sv2a+/+ | RNA-Seq | TRANSCRIPTOMIC | PolyA | SINGLE | ILLUMINA | NextSeq 500 | ERP135966 | NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 | sv2aWT1S1L003-1.fastq.gz | fastq | 654781451.0 | 8669333.0 | E MTAB 11505:sv2aWT1S1L003 | 0:75.53 1:0 | A:156252744;C:162396721;G:155681450;T:180352911;N:97625 | 75 | 0 | 156252744 | 162396721 | 155681450 | 180352911 | 97625 | ERX8620333 | ERS10857221 | ERA9644804 | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive | 1 | 0.95972 | 0.08498 | 0.66137 | 0.47457 | 75 | B | usable mapping rate | illumina | nextseq | unknown | poly_a | trueseq | bulk | unknown | unknown | Belgium | 2022-04-21 | Larval | Larval | Head | Nervous System | |||||||||||||||||||
| 10422 | 10422 | ERR9077193 | ERX8620333 | ERS10857221 | ERP135966 | PRJEB51339 | RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | E-MTAB-11505 | Transcriptome Analysis | Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. | ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 | Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. | Sv2aWT1S1 | SAMEA13254178 | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg | ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254178|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aWT1S1|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/+|individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aWT1S1|sex:not available|strain:AB | NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | E MTAB 11505:Sv2aWT1S1 s | Sv2aWT1S1 s | RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. | Experimental Factor: genotype:sv2a+/+ | RNA-Seq | TRANSCRIPTOMIC | PolyA | SINGLE | ILLUMINA | NextSeq 500 | ERP135966 | NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 | sv2aWT1S1L004-1.fastq.gz | fastq | 659716937.0 | 8734618.0 | E MTAB 11505:sv2aWT1S1L004 | 0:75.53 1:0 | A:157367211;C:163585842;G:156911139;T:181757809;N:94936 | 75 | 0 | 157367211 | 163585842 | 156911139 | 181757809 | 94936 | ERX8620333 | ERS10857221 | ERA9644804 | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive | 1 | 0.95985 | 0.08585 | 0.65857 | 0.47349 | 76 | B | usable mapping rate | illumina | nextseq | unknown | poly_a | trueseq | bulk | unknown | unknown | Belgium | 2022-04-21 | Larval | Larval | Head | Nervous System | |||||||||||||||||||
| 10423 | 10423 | ERR9077186 | ERX8620332 | ERS10857220 | ERP135966 | PRJEB51339 | RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | E-MTAB-11505 | Transcriptome Analysis | Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. | ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 | Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. | Sv2aHO6S12 | SAMEA13254177 | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg | ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254177|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHO6S12|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a / |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHO6S12|sex:not available|strain:AB | NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | E MTAB 11505:Sv2aHO6S12 s | Sv2aHO6S12 s | RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. | Experimental Factor: genotype:sv2a / | RNA-Seq | TRANSCRIPTOMIC | PolyA | SINGLE | ILLUMINA | NextSeq 500 | ERP135966 | NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 | sv2aHO6S12L001-1.fastq.gz | fastq | 675018261.0 | 8937958.0 | E MTAB 11505:sv2aHO6S12L001 | 0:75.52 1:0 | A:166564263;C:160539328;G:160003980;T:187773509;N:137181 | 75 | 0 | 166564263 | 160539328 | 160003980 | 187773509 | 137181 | ERX8620332 | ERS10857220 | ERA9644804 | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive | 1 | 0.94964 | 0.13009 | 0.65413 | 0.47079 | 76 | B | usable mapping rate | illumina | nextseq | unknown | poly_a | trueseq | bulk | unknown | unknown | Belgium | 2022-04-21 | Larval | Larval | Head | Nervous System | |||||||||||||||||||
| 10424 | 10424 | ERR9077187 | ERX8620332 | ERS10857220 | ERP135966 | PRJEB51339 | RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | E-MTAB-11505 | Transcriptome Analysis | Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. | ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 | Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. | Sv2aHO6S12 | SAMEA13254177 | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg | ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254177|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHO6S12|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a / |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHO6S12|sex:not available|strain:AB | NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | E MTAB 11505:Sv2aHO6S12 s | Sv2aHO6S12 s | RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. | Experimental Factor: genotype:sv2a / | RNA-Seq | TRANSCRIPTOMIC | PolyA | SINGLE | ILLUMINA | NextSeq 500 | ERP135966 | NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 | sv2aHO6S12L002-1.fastq.gz | fastq | 672995667.0 | 8911161.0 | E MTAB 11505:sv2aHO6S12L002 | 0:75.52 1:0 | A:165990319;C:160039398;G:159722501;T:187108781;N:134668 | 75 | 0 | 165990319 | 160039398 | 159722501 | 187108781 | 134668 | ERX8620332 | ERS10857220 | ERA9644804 | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive | 1 | 0.94837 | 0.13029 | 0.65744 | 0.47959 | 76 | B | usable mapping rate | illumina | nextseq | unknown | poly_a | trueseq | bulk | unknown | unknown | Belgium | 2022-04-21 | Larval | Larval | Head | Nervous System | |||||||||||||||||||
| 10425 | 10425 | ERR9077188 | ERX8620332 | ERS10857220 | ERP135966 | PRJEB51339 | RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | E-MTAB-11505 | Transcriptome Analysis | Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. | ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 | Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. | Sv2aHO6S12 | SAMEA13254177 | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg | ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254177|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHO6S12|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a / |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHO6S12|sex:not available|strain:AB | NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | E MTAB 11505:Sv2aHO6S12 s | Sv2aHO6S12 s | RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. | Experimental Factor: genotype:sv2a / | RNA-Seq | TRANSCRIPTOMIC | PolyA | SINGLE | ILLUMINA | NextSeq 500 | ERP135966 | NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 | sv2aHO6S12L003-1.fastq.gz | fastq | 683276767.0 | 9047147.0 | E MTAB 11505:sv2aHO6S12L003 | 0:75.52 1:0 | A:168501690;C:162583852;G:162063190;T:190018001;N:110034 | 75 | 0 | 168501690 | 162583852 | 162063190 | 190018001 | 110034 | ERX8620332 | ERS10857220 | ERA9644804 | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive | 1 | 0.94983 | 0.13248 | 0.6565 | 0.47977 | 76 | B | usable mapping rate | illumina | nextseq | unknown | poly_a | trueseq | bulk | unknown | unknown | Belgium | 2022-04-21 | Larval | Larval | Head | Nervous System | |||||||||||||||||||
| 10426 | 10426 | ERR9077189 | ERX8620332 | ERS10857220 | ERP135966 | PRJEB51339 | RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | E-MTAB-11505 | Transcriptome Analysis | Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. | ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 | Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. | Sv2aHO6S12 | SAMEA13254177 | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg | ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254177|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHO6S12|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a / |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHO6S12|sex:not available|strain:AB | NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | E MTAB 11505:Sv2aHO6S12 s | Sv2aHO6S12 s | RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. | Experimental Factor: genotype:sv2a / | RNA-Seq | TRANSCRIPTOMIC | PolyA | SINGLE | ILLUMINA | NextSeq 500 | ERP135966 | NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 | sv2aHO6S12L004-1.fastq.gz | fastq | 686469910.0 | 9089431.0 | E MTAB 11505:sv2aHO6S12L004 | 0:75.52 1:0 | A:169287189;C:163276361;G:162844724;T:190954218;N:107418 | 75 | 0 | 169287189 | 163276361 | 162844724 | 190954218 | 107418 | ERX8620332 | ERS10857220 | ERA9644804 | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive | 1 | 0.95061 | 0.13029 | 0.65389 | 0.48044 | 76 | B | usable mapping rate | illumina | nextseq | unknown | poly_a | trueseq | bulk | unknown | unknown | Belgium | 2022-04-21 | Larval | Larval | Head | Nervous System | |||||||||||||||||||
| 10427 | 10427 | ERR9077182 | ERX8620331 | ERS10857219 | ERP135966 | PRJEB51339 | RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | E-MTAB-11505 | Transcriptome Analysis | Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. | ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 | Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. | Sv2aHO4S10 | SAMEA13254176 | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg | ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254176|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHO4S10|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a / |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHO4S10|sex:not available|strain:AB | NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | E MTAB 11505:Sv2aHO4S10 s | Sv2aHO4S10 s | RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. | Experimental Factor: genotype:sv2a / | RNA-Seq | TRANSCRIPTOMIC | PolyA | SINGLE | ILLUMINA | NextSeq 500 | ERP135966 | NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 | sv2aHO4S10L001-1.fastq.gz | fastq | 713858966.0 | 9452474.0 | E MTAB 11505:sv2aHO4S10L001 | 0:75.52 1:0 | A:177913695;C:169425317;G:167540012;T:198835532;N:144410 | 75 | 0 | 177913695 | 169425317 | 167540012 | 198835532 | 144410 | ERX8620331 | ERS10857219 | ERA9644804 | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive | 1 | 0.94848 | 0.12981 | 0.6636 | 0.47772 | 76 | B | usable mapping rate | illumina | nextseq | unknown | poly_a | trueseq | bulk | unknown | unknown | Belgium | 2022-04-21 | Larval | Larval | Head | Nervous System | |||||||||||||||||||
| 10428 | 10428 | ERR9077183 | ERX8620331 | ERS10857219 | ERP135966 | PRJEB51339 | RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | E-MTAB-11505 | Transcriptome Analysis | Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. | ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 | Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. | Sv2aHO4S10 | SAMEA13254176 | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg | ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254176|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHO4S10|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a / |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHO4S10|sex:not available|strain:AB | NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | E MTAB 11505:Sv2aHO4S10 s | Sv2aHO4S10 s | RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. | Experimental Factor: genotype:sv2a / | RNA-Seq | TRANSCRIPTOMIC | PolyA | SINGLE | ILLUMINA | NextSeq 500 | ERP135966 | NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 | sv2aHO4S10L002-1.fastq.gz | fastq | 717619593.0 | 9502301.0 | E MTAB 11505:sv2aHO4S10L002 | 0:75.52 1:0 | A:178830542;C:170284720;G:168594514;T:199773382;N:136435 | 75 | 0 | 178830542 | 170284720 | 168594514 | 199773382 | 136435 | ERX8620331 | ERS10857219 | ERA9644804 | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive | 1 | 0.94812 | 0.12868 | 0.6645 | 0.47281 | 75 | B | usable mapping rate | illumina | nextseq | unknown | poly_a | trueseq | bulk | unknown | unknown | Belgium | 2022-04-21 | Larval | Larval | Head | Nervous System | |||||||||||||||||||
| 10429 | 10429 | ERR9077184 | ERX8620331 | ERS10857219 | ERP135966 | PRJEB51339 | RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | E-MTAB-11505 | Transcriptome Analysis | Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. | ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 | Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. | Sv2aHO4S10 | SAMEA13254176 | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg | ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254176|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHO4S10|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a / |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHO4S10|sex:not available|strain:AB | NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | E MTAB 11505:Sv2aHO4S10 s | Sv2aHO4S10 s | RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. | Experimental Factor: genotype:sv2a / | RNA-Seq | TRANSCRIPTOMIC | PolyA | SINGLE | ILLUMINA | NextSeq 500 | ERP135966 | NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 | sv2aHO4S10L003-1.fastq.gz | fastq | 727263626.0 | 9629858.0 | E MTAB 11505:sv2aHO4S10L003 | 0:75.52 1:0 | A:181201278;C:172619520;G:170780953;T:202550397;N:111478 | 75 | 0 | 181201278 | 172619520 | 170780953 | 202550397 | 111478 | ERX8620331 | ERS10857219 | ERA9644804 | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive | 1 | 0.94768 | 0.12827 | 0.66596 | 0.47332 | 76 | B | usable mapping rate | illumina | nextseq | unknown | poly_a | trueseq | bulk | unknown | unknown | Belgium | 2022-04-21 | Larval | Larval | Head | Nervous System | |||||||||||||||||||
| 10430 | 10430 | ERR9077185 | ERX8620331 | ERS10857219 | ERP135966 | PRJEB51339 | RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | E-MTAB-11505 | Transcriptome Analysis | Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. | ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 | Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. | Sv2aHO4S10 | SAMEA13254176 | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg | ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254176|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHO4S10|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a / |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHO4S10|sex:not available|strain:AB | NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | E MTAB 11505:Sv2aHO4S10 s | Sv2aHO4S10 s | RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. | Experimental Factor: genotype:sv2a / | RNA-Seq | TRANSCRIPTOMIC | PolyA | SINGLE | ILLUMINA | NextSeq 500 | ERP135966 | NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 | sv2aHO4S10L004-1.fastq.gz | fastq | 736595278.0 | 9753337.0 | E MTAB 11505:sv2aHO4S10L004 | 0:75.52 1:0 | A:183510872;C:174806534;G:173027794;T:205140640;N:109438 | 75 | 0 | 183510872 | 174806534 | 173027794 | 205140640 | 109438 | ERX8620331 | ERS10857219 | ERA9644804 | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive | 1 | 0.94925 | 0.12904 | 0.66417 | 0.47297 | 76 | B | usable mapping rate | illumina | nextseq | unknown | poly_a | trueseq | bulk | unknown | unknown | Belgium | 2022-04-21 | Larval | Larval | Head | Nervous System | |||||||||||||||||||
| 10431 | 10431 | ERR9077178 | ERX8620330 | ERS10857218 | ERP135966 | PRJEB51339 | RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | E-MTAB-11505 | Transcriptome Analysis | Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. | ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 | Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. | Sv2aHO3S6 | SAMEA13254175 | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg | ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254175|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHO3S6|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a / |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHO3S6|sex:not available|strain:AB | NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | E MTAB 11505:Sv2aHO3S6 s | Sv2aHO3S6 s | RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. | Experimental Factor: genotype:sv2a / | RNA-Seq | TRANSCRIPTOMIC | PolyA | SINGLE | ILLUMINA | NextSeq 500 | ERP135966 | NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 | sv2aHO3S6L001-1.fastq.gz | fastq | 710756305.0 | 9410862.0 | E MTAB 11505:sv2aHO3S6L001 | 0:75.53 1:0 | A:175585613;C:170261120;G:166658917;T:198106388;N:144267 | 75 | 0 | 175585613 | 170261120 | 166658917 | 198106388 | 144267 | ERX8620330 | ERS10857218 | ERA9644804 | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive | 1 | 0.94827 | 0.14002 | 0.66785 | 0.474 | 75 | B | usable mapping rate | illumina | nextseq | unknown | poly_a | trueseq | bulk | unknown | unknown | Belgium | 2022-04-21 | Larval | Larval | Head | Nervous System | |||||||||||||||||||
| 10432 | 10432 | ERR9077179 | ERX8620330 | ERS10857218 | ERP135966 | PRJEB51339 | RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | E-MTAB-11505 | Transcriptome Analysis | Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. | ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 | Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. | Sv2aHO3S6 | SAMEA13254175 | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg | ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254175|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHO3S6|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a / |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHO3S6|sex:not available|strain:AB | NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | E MTAB 11505:Sv2aHO3S6 s | Sv2aHO3S6 s | RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. | Experimental Factor: genotype:sv2a / | RNA-Seq | TRANSCRIPTOMIC | PolyA | SINGLE | ILLUMINA | NextSeq 500 | ERP135966 | NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 | sv2aHO3S6L002-1.fastq.gz | fastq | 715330324.0 | 9471415.0 | E MTAB 11505:sv2aHO3S6L002 | 0:75.53 1:0 | A:176679775;C:171311031;G:167903237;T:199299483;N:136798 | 75 | 0 | 176679775 | 171311031 | 167903237 | 199299483 | 136798 | ERX8620330 | ERS10857218 | ERA9644804 | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive | 1 | 0.9469 | 0.13915 | 0.67004 | 0.47654 | 75 | B | usable mapping rate | illumina | nextseq | unknown | poly_a | trueseq | bulk | unknown | unknown | Belgium | 2022-04-21 | Larval | Larval | Head | Nervous System | |||||||||||||||||||
| 10433 | 10433 | ERR9077180 | ERX8620330 | ERS10857218 | ERP135966 | PRJEB51339 | RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | E-MTAB-11505 | Transcriptome Analysis | Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. | ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 | Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. | Sv2aHO3S6 | SAMEA13254175 | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg | ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254175|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHO3S6|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a / |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHO3S6|sex:not available|strain:AB | NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | E MTAB 11505:Sv2aHO3S6 s | Sv2aHO3S6 s | RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. | Experimental Factor: genotype:sv2a / | RNA-Seq | TRANSCRIPTOMIC | PolyA | SINGLE | ILLUMINA | NextSeq 500 | ERP135966 | NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 | sv2aHO3S6L003-1.fastq.gz | fastq | 722810562.0 | 9570364.0 | E MTAB 11505:sv2aHO3S6L003 | 0:75.53 1:0 | A:178442845;C:173192786;G:169595085;T:201465522;N:114324 | 75 | 0 | 178442845 | 173192786 | 169595085 | 201465522 | 114324 | ERX8620330 | ERS10857218 | ERA9644804 | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive | 1 | 0.94918 | 0.13979 | 0.66902 | 0.47572 | 75 | B | usable mapping rate | illumina | nextseq | unknown | poly_a | trueseq | bulk | unknown | unknown | Belgium | 2022-04-21 | Larval | Larval | Head | Nervous System | |||||||||||||||||||
| 10434 | 10434 | ERR9077181 | ERX8620330 | ERS10857218 | ERP135966 | PRJEB51339 | RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | E-MTAB-11505 | Transcriptome Analysis | Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. | ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 | Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. | Sv2aHO3S6 | SAMEA13254175 | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg | ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254175|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHO3S6|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a / |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHO3S6|sex:not available|strain:AB | NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | E MTAB 11505:Sv2aHO3S6 s | Sv2aHO3S6 s | RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. | Experimental Factor: genotype:sv2a / | RNA-Seq | TRANSCRIPTOMIC | PolyA | SINGLE | ILLUMINA | NextSeq 500 | ERP135966 | NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 | sv2aHO3S6L004-1.fastq.gz | fastq | 732990507.0 | 9705054.0 | E MTAB 11505:sv2aHO3S6L004 | 0:75.53 1:0 | A:180951130;C:175567778;G:172037542;T:204323488;N:110569 | 75 | 0 | 180951130 | 175567778 | 172037542 | 204323488 | 110569 | ERX8620330 | ERS10857218 | ERA9644804 | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive | 1 | 0.94812 | 0.13893 | 0.67085 | 0.47502 | 75 | B | usable mapping rate | illumina | nextseq | unknown | poly_a | trueseq | bulk | unknown | unknown | Belgium | 2022-04-21 | Larval | Larval | Head | Nervous System | |||||||||||||||||||
| 10435 | 10435 | ERR9077174 | ERX8620329 | ERS10857217 | ERP135966 | PRJEB51339 | RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | E-MTAB-11505 | Transcriptome Analysis | Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. | ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 | Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. | Sv2aHET7S9 | SAMEA13254174 | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg | ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254174|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHET7S9|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/ |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHET7S9|sex:not available|strain:AB | NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | E MTAB 11505:Sv2aHET7S9 s | Sv2aHET7S9 s | RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. | Experimental Factor: genotype:sv2a+/ | RNA-Seq | TRANSCRIPTOMIC | PolyA | SINGLE | ILLUMINA | NextSeq 500 | ERP135966 | NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 | sv2aHET7S9L001-1.fastq.gz | fastq | 773209432.0 | 10238087.0 | E MTAB 11505:sv2aHET7S9L001 | 0:75.52 1:0 | A:191962953;C:184699376;G:180774008;T:215620759;N:152336 | 75 | 0 | 191962953 | 184699376 | 180774008 | 215620759 | 152336 | ERX8620329 | ERS10857217 | ERA9644804 | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive | 1 | 0.95047 | 0.12485 | 0.65705 | 0.4775 | 76 | B | usable mapping rate | illumina | nextseq | unknown | poly_a | trueseq | bulk | unknown | unknown | Belgium | 2022-04-21 | Larval | Larval | Head | Nervous System | |||||||||||||||||||
| 10436 | 10436 | ERR9077175 | ERX8620329 | ERS10857217 | ERP135966 | PRJEB51339 | RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | E-MTAB-11505 | Transcriptome Analysis | Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. | ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 | Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. | Sv2aHET7S9 | SAMEA13254174 | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg | ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254174|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHET7S9|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/ |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHET7S9|sex:not available|strain:AB | NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | E MTAB 11505:Sv2aHET7S9 s | Sv2aHET7S9 s | RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. | Experimental Factor: genotype:sv2a+/ | RNA-Seq | TRANSCRIPTOMIC | PolyA | SINGLE | ILLUMINA | NextSeq 500 | ERP135966 | NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 | sv2aHET7S9L002-1.fastq.gz | fastq | 773106152.0 | 10236677.0 | E MTAB 11505:sv2aHET7S9L002 | 0:75.52 1:0 | A:191812833;C:184676419;G:180997547;T:215473732;N:145621 | 75 | 0 | 191812833 | 184676419 | 180997547 | 215473732 | 145621 | ERX8620329 | ERS10857217 | ERA9644804 | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive | 1 | 0.95016 | 0.12464 | 0.65482 | 0.47419 | 76 | B | usable mapping rate | illumina | nextseq | unknown | poly_a | trueseq | bulk | unknown | unknown | Belgium | 2022-04-21 | Larval | Larval | Head | Nervous System | |||||||||||||||||||
| 10437 | 10437 | ERR9077176 | ERX8620329 | ERS10857217 | ERP135966 | PRJEB51339 | RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | E-MTAB-11505 | Transcriptome Analysis | Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. | ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 | Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. | Sv2aHET7S9 | SAMEA13254174 | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg | ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254174|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHET7S9|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/ |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHET7S9|sex:not available|strain:AB | NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | E MTAB 11505:Sv2aHET7S9 s | Sv2aHET7S9 s | RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. | Experimental Factor: genotype:sv2a+/ | RNA-Seq | TRANSCRIPTOMIC | PolyA | SINGLE | ILLUMINA | NextSeq 500 | ERP135966 | NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 | sv2aHET7S9L003-1.fastq.gz | fastq | 783061051.0 | 10368445.0 | E MTAB 11505:sv2aHET7S9L003 | 0:75.52 1:0 | A:194289152;C:187108364;G:183177036;T:218370500;N:115999 | 75 | 0 | 194289152 | 187108364 | 183177036 | 218370500 | 115999 | ERX8620329 | ERS10857217 | ERA9644804 | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive | 1 | 0.95172 | 0.12488 | 0.65445 | 0.48157 | 76 | B | usable mapping rate | illumina | nextseq | unknown | poly_a | trueseq | bulk | unknown | unknown | Belgium | 2022-04-21 | Larval | Larval | Head | Nervous System | |||||||||||||||||||
| 10438 | 10438 | ERR9077177 | ERX8620329 | ERS10857217 | ERP135966 | PRJEB51339 | RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | E-MTAB-11505 | Transcriptome Analysis | Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. | ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 | Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. | Sv2aHET7S9 | SAMEA13254174 | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg | ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254174|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHET7S9|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/ |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHET7S9|sex:not available|strain:AB | NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | E MTAB 11505:Sv2aHET7S9 s | Sv2aHET7S9 s | RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. | Experimental Factor: genotype:sv2a+/ | RNA-Seq | TRANSCRIPTOMIC | PolyA | SINGLE | ILLUMINA | NextSeq 500 | ERP135966 | NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 | sv2aHET7S9L004-1.fastq.gz | fastq | 790150025.0 | 10462214.0 | E MTAB 11505:sv2aHET7S9L004 | 0:75.52 1:0 | A:196056500;C:188746833;G:184927588;T:220305922;N:113182 | 75 | 0 | 196056500 | 188746833 | 184927588 | 220305922 | 113182 | ERX8620329 | ERS10857217 | ERA9644804 | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive | 1 | 0.95285 | 0.12478 | 0.65551 | 0.4763 | 76 | B | usable mapping rate | illumina | nextseq | unknown | poly_a | trueseq | bulk | unknown | unknown | Belgium | 2022-04-21 | Larval | Larval | Head | Nervous System | |||||||||||||||||||
| 10439 | 10439 | ERR9077170 | ERX8620328 | ERS10857216 | ERP135966 | PRJEB51339 | RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | E-MTAB-11505 | Transcriptome Analysis | Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. | ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 | Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. | Sv2aHET4S5 | SAMEA13254173 | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg | ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254173|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHET4S5|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/ |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHET4S5|sex:not available|strain:AB | NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | E MTAB 11505:Sv2aHET4S5 s | Sv2aHET4S5 s | RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. | Experimental Factor: genotype:sv2a+/ | RNA-Seq | TRANSCRIPTOMIC | PolyA | SINGLE | ILLUMINA | NextSeq 500 | ERP135966 | NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 | sv2aHET4S5L001-1.fastq.gz | fastq | 685650097.0 | 9076481.0 | E MTAB 11505:sv2aHET4S5L001 | 0:75.54 1:0 | A:161812875;C:167870520;G:166985310;T:188850530;N:130862 | 75 | 0 | 161812875 | 167870520 | 166985310 | 188850530 | 130862 | ERX8620328 | ERS10857216 | ERA9644804 | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive | 1 | 0.95733 | 0.09974 | 0.65691 | 0.47392 | 76 | B | usable mapping rate | illumina | nextseq | unknown | poly_a | trueseq | bulk | unknown | unknown | Belgium | 2022-04-21 | Larval | Larval | Head | Nervous System | |||||||||||||||||||
| 10440 | 10440 | ERR9077171 | ERX8620328 | ERS10857216 | ERP135966 | PRJEB51339 | RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | E-MTAB-11505 | Transcriptome Analysis | Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. | ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 | Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. | Sv2aHET4S5 | SAMEA13254173 | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg | ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254173|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHET4S5|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/ |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHET4S5|sex:not available|strain:AB | NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | E MTAB 11505:Sv2aHET4S5 s | Sv2aHET4S5 s | RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. | Experimental Factor: genotype:sv2a+/ | RNA-Seq | TRANSCRIPTOMIC | PolyA | SINGLE | ILLUMINA | NextSeq 500 | ERP135966 | NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 | sv2aHET4S5L002-1.fastq.gz | fastq | 684402241.0 | 9059974.0 | E MTAB 11505:sv2aHET4S5L002 | 0:75.54 1:0 | A:161493428;C:167520248;G:166882054;T:188383816;N:122695 | 75 | 0 | 161493428 | 167520248 | 166882054 | 188383816 | 122695 | ERX8620328 | ERS10857216 | ERA9644804 | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive | 1 | 0.95537 | 0.09915 | 0.65719 | 0.48261 | 76 | B | usable mapping rate | illumina | nextseq | unknown | poly_a | trueseq | bulk | unknown | unknown | Belgium | 2022-04-21 | Larval | Larval | Head | Nervous System | |||||||||||||||||||
| 10441 | 10441 | ERR9077172 | ERX8620328 | ERS10857216 | ERP135966 | PRJEB51339 | RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | E-MTAB-11505 | Transcriptome Analysis | Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. | ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 | Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. | Sv2aHET4S5 | SAMEA13254173 | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg | ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254173|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHET4S5|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/ |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHET4S5|sex:not available|strain:AB | NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | E MTAB 11505:Sv2aHET4S5 s | Sv2aHET4S5 s | RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. | Experimental Factor: genotype:sv2a+/ | RNA-Seq | TRANSCRIPTOMIC | PolyA | SINGLE | ILLUMINA | NextSeq 500 | ERP135966 | NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 | sv2aHET4S5L003-1.fastq.gz | fastq | 694099126.0 | 9188245.0 | E MTAB 11505:sv2aHET4S5L003 | 0:75.54 1:0 | A:163727378;C:169989210;G:169143224;T:191137588;N:101726 | 75 | 0 | 163727378 | 169989210 | 169143224 | 191137588 | 101726 | ERX8620328 | ERS10857216 | ERA9644804 | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive | 1 | 0.95629 | 0.09861 | 0.65705 | 0.47187 | 75 | B | usable mapping rate | illumina | nextseq | unknown | poly_a | trueseq | bulk | unknown | unknown | Belgium | 2022-04-21 | Larval | Larval | Head | Nervous System | |||||||||||||||||||
| 10442 | 10442 | ERR9077173 | ERX8620328 | ERS10857216 | ERP135966 | PRJEB51339 | RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | E-MTAB-11505 | Transcriptome Analysis | Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. | ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 | Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. | Sv2aHET4S5 | SAMEA13254173 | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg | ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254173|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHET4S5|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/ |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHET4S5|sex:not available|strain:AB | NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | E MTAB 11505:Sv2aHET4S5 s | Sv2aHET4S5 s | RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. | Experimental Factor: genotype:sv2a+/ | RNA-Seq | TRANSCRIPTOMIC | PolyA | SINGLE | ILLUMINA | NextSeq 500 | ERP135966 | NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 | sv2aHET4S5L004-1.fastq.gz | fastq | 698753836.0 | 9249820.0 | E MTAB 11505:sv2aHET4S5L004 | 0:75.54 1:0 | A:164809473;C:171057633;G:170349692;T:192441413;N:95625 | 75 | 0 | 164809473 | 171057633 | 170349692 | 192441413 | 95625 | ERX8620328 | ERS10857216 | ERA9644804 | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive | 1 | 0.95613 | 0.09885 | 0.65798 | 0.47227 | 76 | B | usable mapping rate | illumina | nextseq | unknown | poly_a | trueseq | bulk | unknown | unknown | Belgium | 2022-04-21 | Larval | Larval | Head | Nervous System | |||||||||||||||||||
| 10443 | 10443 | ERR9077166 | ERX8620327 | ERS10857215 | ERP135966 | PRJEB51339 | RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | E-MTAB-11505 | Transcriptome Analysis | Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. | ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 | Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. | Sv2aHET3S4 | SAMEA13254172 | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg | ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254172|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHET3S4|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/ |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHET3S4|sex:not available|strain:AB | NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | E MTAB 11505:Sv2aHET3S4 s | Sv2aHET3S4 s | RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. | Experimental Factor: genotype:sv2a+/ | RNA-Seq | TRANSCRIPTOMIC | PolyA | SINGLE | ILLUMINA | NextSeq 500 | ERP135966 | NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 | sv2aHET3S4L001-1.fastq.gz | fastq | 691298605.0 | 9151512.0 | E MTAB 11505:sv2aHET3S4L001 | 0:75.54 1:0 | A:164743919;C:170752099;G:164763621;T:190912044;N:126922 | 75 | 0 | 164743919 | 170752099 | 164763621 | 190912044 | 126922 | ERX8620327 | ERS10857215 | ERA9644804 | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive | 1 | 0.95684 | 0.09604 | 0.6603 | 0.47285 | 76 | B | usable mapping rate | illumina | nextseq | unknown | poly_a | trueseq | bulk | unknown | unknown | Belgium | 2022-04-21 | Larval | Larval | Head | Nervous System | |||||||||||||||||||
| 10444 | 10444 | ERR9077167 | ERX8620327 | ERS10857215 | ERP135966 | PRJEB51339 | RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | E-MTAB-11505 | Transcriptome Analysis | Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. | ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 | Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. | Sv2aHET3S4 | SAMEA13254172 | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg | ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254172|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHET3S4|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/ |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHET3S4|sex:not available|strain:AB | NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | E MTAB 11505:Sv2aHET3S4 s | Sv2aHET3S4 s | RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. | Experimental Factor: genotype:sv2a+/ | RNA-Seq | TRANSCRIPTOMIC | PolyA | SINGLE | ILLUMINA | NextSeq 500 | ERP135966 | NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 | sv2aHET3S4L002-1.fastq.gz | fastq | 694080975.0 | 9188259.0 | E MTAB 11505:sv2aHET3S4L002 | 0:75.54 1:0 | A:165336810;C:171397273;G:165632321;T:191591872;N:122699 | 75 | 0 | 165336810 | 171397273 | 165632321 | 191591872 | 122699 | ERX8620327 | ERS10857215 | ERA9644804 | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive | 1 | 0.95612 | 0.09694 | 0.65993 | 0.46918 | 76 | B | usable mapping rate | illumina | nextseq | unknown | poly_a | trueseq | bulk | unknown | unknown | Belgium | 2022-04-21 | Larval | Larval | Head | Nervous System | |||||||||||||||||||
| 10445 | 10445 | ERR9077168 | ERX8620327 | ERS10857215 | ERP135966 | PRJEB51339 | RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | E-MTAB-11505 | Transcriptome Analysis | Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. | ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 | Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. | Sv2aHET3S4 | SAMEA13254172 | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg | ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254172|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHET3S4|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/ |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHET3S4|sex:not available|strain:AB | NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | E MTAB 11505:Sv2aHET3S4 s | Sv2aHET3S4 s | RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. | Experimental Factor: genotype:sv2a+/ | RNA-Seq | TRANSCRIPTOMIC | PolyA | SINGLE | ILLUMINA | NextSeq 500 | ERP135966 | NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 | sv2aHET3S4L003-1.fastq.gz | fastq | 703689889.0 | 9315387.0 | E MTAB 11505:sv2aHET3S4L003 | 0:75.54 1:0 | A:167575230;C:173881977;G:167845574;T:194289403;N:97705 | 75 | 0 | 167575230 | 173881977 | 167845574 | 194289403 | 97705 | ERX8620327 | ERS10857215 | ERA9644804 | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive | 1 | 0.9577 | 0.09608 | 0.66042 | 0.47022 | 74 | B | usable mapping rate | illumina | nextseq | unknown | poly_a | trueseq | bulk | unknown | unknown | Belgium | 2022-04-21 | Larval | Larval | Head | Nervous System | |||||||||||||||||||
| 10446 | 10446 | ERR9077169 | ERX8620327 | ERS10857215 | ERP135966 | PRJEB51339 | RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | E-MTAB-11505 | Transcriptome Analysis | Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. | ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 | Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. | Sv2aHET3S4 | SAMEA13254172 | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg | ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254172|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHET3S4|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/ |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHET3S4|sex:not available|strain:AB | NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | E MTAB 11505:Sv2aHET3S4 s | Sv2aHET3S4 s | RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. | Experimental Factor: genotype:sv2a+/ | RNA-Seq | TRANSCRIPTOMIC | PolyA | SINGLE | ILLUMINA | NextSeq 500 | ERP135966 | NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 | sv2aHET3S4L004-1.fastq.gz | fastq | 712116538.0 | 9426961.0 | E MTAB 11505:sv2aHET3S4L004 | 0:75.54 1:0 | A:169598596;C:175885948;G:169866534;T:196669400;N:96060 | 75 | 0 | 169598596 | 175885948 | 169866534 | 196669400 | 96060 | ERX8620327 | ERS10857215 | ERA9644804 | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive | 1 | 0.95734 | 0.09716 | 0.66054 | 0.47257 | 75 | B | usable mapping rate | illumina | nextseq | unknown | poly_a | trueseq | bulk | unknown | unknown | Belgium | 2022-04-21 | Larval | Larval | Head | Nervous System | |||||||||||||||||||
| 10447 | 10447 | ERR9077162 | ERX8620326 | ERS10857214 | ERP135966 | PRJEB51339 | RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | E-MTAB-11505 | Transcriptome Analysis | Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. | ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 | Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. | Sv2aHET2S3 | SAMEA13254171 | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg | ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254171|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHET2S3|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/ |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHET2S3|sex:not available|strain:AB | NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | E MTAB 11505:Sv2aHET2S3 s | Sv2aHET2S3 s | RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. | Experimental Factor: genotype:sv2a+/ | RNA-Seq | TRANSCRIPTOMIC | PolyA | SINGLE | ILLUMINA | NextSeq 500 | ERP135966 | NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 | sv2aHET2S3L001-1.fastq.gz | fastq | 733440522.0 | 9709422.0 | E MTAB 11505:sv2aHET2S3L001 | 0:75.54 1:0 | A:173670000;C:181175353;G:175392793;T:203059256;N:143120 | 75 | 0 | 173670000 | 181175353 | 175392793 | 203059256 | 143120 | ERX8620326 | ERS10857214 | ERA9644804 | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive | 1 | 0.95885 | 0.08758 | 0.65393 | 0.47249 | 76 | B | usable mapping rate | illumina | nextseq | unknown | poly_a | trueseq | bulk | unknown | unknown | Belgium | 2022-04-21 | Larval | Larval | Head | Nervous System | |||||||||||||||||||
| 10448 | 10448 | ERR9077163 | ERX8620326 | ERS10857214 | ERP135966 | PRJEB51339 | RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | E-MTAB-11505 | Transcriptome Analysis | Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. | ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 | Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. | Sv2aHET2S3 | SAMEA13254171 | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg | ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254171|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHET2S3|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/ |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHET2S3|sex:not available|strain:AB | NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | E MTAB 11505:Sv2aHET2S3 s | Sv2aHET2S3 s | RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. | Experimental Factor: genotype:sv2a+/ | RNA-Seq | TRANSCRIPTOMIC | PolyA | SINGLE | ILLUMINA | NextSeq 500 | ERP135966 | NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 | sv2aHET2S3L002-1.fastq.gz | fastq | 732121929.0 | 9691967.0 | E MTAB 11505:sv2aHET2S3L002 | 0:75.54 1:0 | A:173332091;C:180805738;G:175271736;T:202574727;N:137637 | 75 | 0 | 173332091 | 180805738 | 175271736 | 202574727 | 137637 | ERX8620326 | ERS10857214 | ERA9644804 | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive | 1 | 0.95775 | 0.08879 | 0.6564 | 0.4718 | 76 | B | usable mapping rate | illumina | nextseq | unknown | poly_a | trueseq | bulk | unknown | unknown | Belgium | 2022-04-21 | Larval | Larval | Head | Nervous System | |||||||||||||||||||
| 10449 | 10449 | ERR9077164 | ERX8620326 | ERS10857214 | ERP135966 | PRJEB51339 | RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | E-MTAB-11505 | Transcriptome Analysis | Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. | ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 | Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. | Sv2aHET2S3 | SAMEA13254171 | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg | ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254171|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHET2S3|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/ |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHET2S3|sex:not available|strain:AB | NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | E MTAB 11505:Sv2aHET2S3 s | Sv2aHET2S3 s | RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. | Experimental Factor: genotype:sv2a+/ | RNA-Seq | TRANSCRIPTOMIC | PolyA | SINGLE | ILLUMINA | NextSeq 500 | ERP135966 | NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 | sv2aHET2S3L003-1.fastq.gz | fastq | 743488189.0 | 9842286.0 | E MTAB 11505:sv2aHET2S3L003 | 0:75.54 1:0 | A:175979238;C:183726319;G:177933958;T:205735955;N:112719 | 75 | 0 | 175979238 | 183726319 | 177933958 | 205735955 | 112719 | ERX8620326 | ERS10857214 | ERA9644804 | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive | 1 | 0.9593 | 0.08716 | 0.65437 | 0.46976 | 75 | B | usable mapping rate | illumina | nextseq | unknown | poly_a | trueseq | bulk | unknown | unknown | Belgium | 2022-04-21 | Larval | Larval | Head | Nervous System | |||||||||||||||||||
| 10450 | 10450 | ERR9077165 | ERX8620326 | ERS10857214 | ERP135966 | PRJEB51339 | RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | E-MTAB-11505 | Transcriptome Analysis | Transcriptome analysis of the heads of 6 dpf sv2a / sv2a+/ and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a / zebrafish larvae. | ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 | Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. | Sv2aHET2S3 | SAMEA13254171 | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg | ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254171|INSDC center alias:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery KU Leuven Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHET2S3|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/ |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHET2S3|sex:not available|strain:AB | NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | E MTAB 11505:Sv2aHET2S3 s | Sv2aHET2S3 s | RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes the RNA pellet was air dried dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines. | Experimental Factor: genotype:sv2a+/ | RNA-Seq | TRANSCRIPTOMIC | PolyA | SINGLE | ILLUMINA | NextSeq 500 | ERP135966 | NextSeq 500 sequencing; RNA seq experiment of sv2a / sv2a+/ and sv2a+/+ zebrafish larvae | ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21 | sv2aHET2S3L004-1.fastq.gz | fastq | 747723481.0 | 9898304.0 | E MTAB 11505:sv2aHET2S3L004 | 0:75.54 1:0 | A:176935451;C:184684782;G:179046040;T:206946124;N:111084 | 75 | 0 | 176935451 | 184684782 | 179046040 | 206946124 | 111084 | ERX8620326 | ERS10857214 | ERA9644804 | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive | Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive | 1 | 0.95934 | 0.08676 | 0.65533 | 0.47523 | 75 | B | usable mapping rate | illumina | nextseq | unknown | poly_a | trueseq | bulk | unknown | unknown | Belgium | 2022-04-21 | Larval | Larval | Head | Nervous System |
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CREATE TABLE run_metadata("run.accession" VARCHAR, "experiment.accession" VARCHAR, "sample.accession" VARCHAR, "study.accession" VARCHAR, bioproject VARCHAR, "study.title" VARCHAR, "study.alias" VARCHAR, "study.type" VARCHAR, "study.abstract" VARCHAR, "study.attributes" VARCHAR, "study.PMIDs" VARCHAR, "sample.description" VARCHAR, "sample.title" VARCHAR, "sample.alias" VARCHAR, "sample.centername" VARCHAR, "sample.attributes" VARCHAR, "GEOsample.title" VARCHAR, "GEOsample.dataprocessing" VARCHAR, "GEOsample.source" VARCHAR, "GEOsample.treatmentprotocol" VARCHAR, "GEOsample.extractprotocol" VARCHAR, "GEOsample.growthprotocol" VARCHAR, "GEOsample.characteristics" VARCHAR, "GEOsample.accession" VARCHAR, "experiment.title" VARCHAR, "experiment.alias" VARCHAR, "experiment.library_name" VARCHAR, "experiment.design_description" VARCHAR, "experiment.library_construction_protocol" VARCHAR, "experiment.attributes" VARCHAR, "experiment.library_strategy" VARCHAR, "experiment.library_source" VARCHAR, "experiment.library_selection" VARCHAR, "experiment.library_layout" VARCHAR, "experiment.platform" VARCHAR, "experiment.instrument_model" VARCHAR, "experiment.spot_descriptor" VARCHAR, "experiment.study_ref" VARCHAR, "run.title" VARCHAR, "run.attributes" VARCHAR, "run.filename" VARCHAR, "run.semantic_name" VARCHAR, "run.total_bases" DOUBLE, "run.total_spots" DOUBLE, "run.alias" VARCHAR, "run.read_lengths" VARCHAR, "run.base_counts" VARCHAR, "run.r1_length" BIGINT, "run.r2_length" BIGINT, "run.r3_length" BIGINT, "run.r4_length" BIGINT, "run.Acount" BIGINT, "run.Ccount" BIGINT, "run.Gcount" BIGINT, "run.Tcount" BIGINT, "run.Ncount" BIGINT, "run.experiment" VARCHAR, "run.pool_member" VARCHAR, "submission.accession" VARCHAR, "submission.srasource" VARCHAR, "submission.bioprojectsource" VARCHAR, "seqdetective.n_mates" BIGINT, "seqdetective.mapping_rate.mate1" DOUBLE, "seqdetective.mapping_rate.mate2" DOUBLE, "seqdetective.nofeature_rate.mate1" DOUBLE, "seqdetective.nofeature_rate.mate2" DOUBLE, "seqdetective.sparsity.mate1" DOUBLE, "seqdetective.sparsity.mate2" DOUBLE, "seqdetective.pos_strand_rate.mate1" DOUBLE, "seqdetective.pos_strand_rate.mate2" DOUBLE, "seqdetective.readlen.mate1" BIGINT, "seqdetective.readlen.mate2" BIGINT, "seqdetective.judgement.mate1" VARCHAR, "seqdetective.judgement.mate2" VARCHAR, "seqdetective.judgement.reason" VARCHAR, platform_family VARCHAR, instrument_generation VARCHAR, read_bias VARCHAR, selection_class VARCHAR, prep_kit VARCHAR, sc_or_bulk VARCHAR, tech_class VARCHAR, technology VARCHAR, tech_variant VARCHAR, "submission.bioprojectsource.country" VARCHAR, earliest_date DATE, devstage_curation VARCHAR, devstage_curation_coarse VARCHAR, tissue_curation VARCHAR, tissue_curation_coarse VARCHAR);;
CREATE INDEX idx_run_bioproject ON run_metadata(bioproject);;
CREATE INDEX idx_run_run_accession ON run_metadata("run.accession");;