run_metadata
57 rows where experiment.library_layout = "SINGLE", experiment.library_selection = "Oligo-dT" and tissue_curation_coarse = "Nervous System"
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| Link | rowid ▼ | run.accession | experiment.accession | sample.accession | study.accession | bioproject | study.title | study.alias | study.type | study.abstract | study.attributes | study.PMIDs | sample.description | sample.title | sample.alias | sample.centername | sample.attributes | GEOsample.title | GEOsample.dataprocessing | GEOsample.source | GEOsample.treatmentprotocol | GEOsample.extractprotocol | GEOsample.growthprotocol | GEOsample.characteristics | GEOsample.accession | experiment.title | experiment.alias | experiment.library_name | experiment.design_description | experiment.library_construction_protocol | experiment.attributes | experiment.library_strategy | experiment.library_source | experiment.library_selection | experiment.library_layout | experiment.platform | experiment.instrument_model | experiment.spot_descriptor | experiment.study_ref | run.title | run.attributes | run.filename | run.semantic_name | run.total_bases | run.total_spots | run.alias | run.read_lengths | run.base_counts | run.r1_length | run.r2_length | run.r3_length | run.r4_length | run.Acount | run.Ccount | run.Gcount | run.Tcount | run.Ncount | run.experiment | run.pool_member | submission.accession | submission.srasource | submission.bioprojectsource | seqdetective.n_mates | seqdetective.mapping_rate.mate1 | seqdetective.mapping_rate.mate2 | seqdetective.nofeature_rate.mate1 | seqdetective.nofeature_rate.mate2 | seqdetective.sparsity.mate1 | seqdetective.sparsity.mate2 | seqdetective.pos_strand_rate.mate1 | seqdetective.pos_strand_rate.mate2 | seqdetective.readlen.mate1 | seqdetective.readlen.mate2 | seqdetective.judgement.mate1 | seqdetective.judgement.mate2 | seqdetective.judgement.reason | platform_family | instrument_generation | read_bias | selection_class | prep_kit | sc_or_bulk | tech_class | technology | tech_variant | submission.bioprojectsource.country | earliest_date | devstage_curation | devstage_curation_coarse | tissue_curation | tissue_curation_coarse |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 10237 | 10237 | ERR7131169 | ERX6698608 | ERS8070397 | ERP132560 | PRJEB48218 | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E-MTAB-11083 | Transcriptome Analysis | To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb. | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | F8 Nega | SAMEA10418613 | Friedrich Miescher Institute for Biomedical Research | ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418613|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F8 Nega|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry /GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 8|organism part:olfactory bulb|sample name:E MTAB 11083:F8 Nega|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E MTAB 11083:F8 Nega s | F8 Nega s | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | Experimental Factor: fraction:mCherry /GFP | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP132560 | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | 2874F22-1_210715_D00404_0538_BCD91CANXX_TCGACGTC-CTAAGCCT_L006_R1_001.fastq.gz | fastq | 658093749.0 | 12903799.0 | E MTAB 11083:2874F22 1 210715 D00404 0538 BCD91CANXX TCGACGTC CTAAGCCT L006 | 0:51 1:0 | A:173355262;C:152464129;G:147489024;T:184739885;N:45449 | 51 | 0 | 173355262 | 152464129 | 147489024 | 184739885 | 45449 | ERX6698608 | ERS8070397 | ERA6757553 | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | 1 | 0.78328 | 0.15147 | 0.71289 | 0.53671 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | poly_a | nextera | sc | single_cell_plate | smartseq | Switzerland | 2021-12-01 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||
| 10238 | 10238 | ERR7131170 | ERX6698608 | ERS8070397 | ERP132560 | PRJEB48218 | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E-MTAB-11083 | Transcriptome Analysis | To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb. | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | F8 Nega | SAMEA10418613 | Friedrich Miescher Institute for Biomedical Research | ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418613|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F8 Nega|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry /GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 8|organism part:olfactory bulb|sample name:E MTAB 11083:F8 Nega|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E MTAB 11083:F8 Nega s | F8 Nega s | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | Experimental Factor: fraction:mCherry /GFP | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP132560 | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | 2874F22-2_210715_D00404_0538_BCD91CANXX_TCGACGTC-CTAAGCCT_L007_R1_001.fastq.gz | fastq | 661103769.0 | 12962819.0 | E MTAB 11083:2874F22 2 210715 D00404 0538 BCD91CANXX TCGACGTC CTAAGCCT L007 | 0:51 1:0 | A:174223249;C:153223284;G:148276805;T:185335092;N:45339 | 51 | 0 | 174223249 | 153223284 | 148276805 | 185335092 | 45339 | ERX6698608 | ERS8070397 | ERA6757553 | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | 1 | 0.78539 | 0.15054 | 0.70897 | 0.5322 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | poly_a | nextera | sc | single_cell_plate | smartseq | Switzerland | 2021-12-01 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||
| 10239 | 10239 | ERR7131167 | ERX6698607 | ERS8070396 | ERP132560 | PRJEB48218 | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E-MTAB-11083 | Transcriptome Analysis | To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb. | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | F8 mCherry GFP | SAMEA10418612 | Friedrich Miescher Institute for Biomedical Research | ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418612|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F8 mCherry GFP|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP+|genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 8|organism part:olfactory bulb|sample name:E MTAB 11083:F8 mCherry GFP|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E MTAB 11083:F8 mCherry GFP s | F8 mCherry GFP s | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | Experimental Factor: fraction:mCherry+/GFP+ | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP132560 | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | 2874F24-1_210715_D00404_0538_BCD91CANXX_TCGACGTC-TCTCTCCG_L006_R1_001.fastq.gz | fastq | 693183636.0 | 13591836.0 | E MTAB 11083:2874F24 1 210715 D00404 0538 BCD91CANXX TCGACGTC TCTCTCCG L006 | 0:51 1:0 | A:183977687;C:159275153;G:153149826;T:196732022;N:48948 | 51 | 0 | 183977687 | 159275153 | 153149826 | 196732022 | 48948 | ERX6698607 | ERS8070396 | ERA6757553 | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | 1 | 0.68942 | 0.15593 | 0.75828 | 0.51858 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | poly_a | nextera | sc | single_cell_plate | smartseq | Switzerland | 2021-12-01 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||
| 10240 | 10240 | ERR7131168 | ERX6698607 | ERS8070396 | ERP132560 | PRJEB48218 | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E-MTAB-11083 | Transcriptome Analysis | To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb. | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | F8 mCherry GFP | SAMEA10418612 | Friedrich Miescher Institute for Biomedical Research | ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418612|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F8 mCherry GFP|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP+|genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 8|organism part:olfactory bulb|sample name:E MTAB 11083:F8 mCherry GFP|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E MTAB 11083:F8 mCherry GFP s | F8 mCherry GFP s | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | Experimental Factor: fraction:mCherry+/GFP+ | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP132560 | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | 2874F24-2_210715_D00404_0538_BCD91CANXX_TCGACGTC-TCTCTCCG_L007_R1_001.fastq.gz | fastq | 696648678.0 | 13659778.0 | E MTAB 11083:2874F24 2 210715 D00404 0538 BCD91CANXX TCGACGTC TCTCTCCG L007 | 0:51 1:0 | A:184971832;C:160126524;G:154012023;T:197490098;N:48201 | 51 | 0 | 184971832 | 160126524 | 154012023 | 197490098 | 48201 | ERX6698607 | ERS8070396 | ERA6757553 | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | 1 | 0.6906 | 0.15634 | 0.75909 | 0.51346 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | poly_a | nextera | sc | single_cell_plate | smartseq | Switzerland | 2021-12-01 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||
| 10241 | 10241 | ERR7131165 | ERX6698606 | ERS8070395 | ERP132560 | PRJEB48218 | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E-MTAB-11083 | Transcriptome Analysis | To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb. | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | F8 mCherry | SAMEA10418611 | Friedrich Miescher Institute for Biomedical Research | ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418611|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F8 mCherry|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 8|organism part:olfactory bulb|sample name:E MTAB 11083:F8 mCherry|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E MTAB 11083:F8 mCherry s | F8 mCherry s | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | Experimental Factor: fraction:mCherry+/GFP | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP132560 | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | 2874F23-1_210715_D00404_0538_BCD91CANXX_TCGACGTC-CGTCTAAT_L006_R1_001.fastq.gz | fastq | 667760646.0 | 13093346.0 | E MTAB 11083:2874F23 1 210715 D00404 0538 BCD91CANXX TCGACGTC CGTCTAAT L006 | 0:51 1:0 | A:179948739;C:150541918;G:145038845;T:192184092;N:47052 | 51 | 0 | 179948739 | 150541918 | 145038845 | 192184092 | 47052 | ERX6698606 | ERS8070395 | ERA6757553 | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | 1 | 0.71661 | 0.19774 | 0.74576 | 0.52117 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | poly_a | nextera | sc | single_cell_plate | smartseq | Switzerland | 2021-12-01 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||
| 10242 | 10242 | ERR7131166 | ERX6698606 | ERS8070395 | ERP132560 | PRJEB48218 | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E-MTAB-11083 | Transcriptome Analysis | To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb. | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | F8 mCherry | SAMEA10418611 | Friedrich Miescher Institute for Biomedical Research | ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418611|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F8 mCherry|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 8|organism part:olfactory bulb|sample name:E MTAB 11083:F8 mCherry|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E MTAB 11083:F8 mCherry s | F8 mCherry s | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | Experimental Factor: fraction:mCherry+/GFP | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP132560 | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | 2874F23-2_210715_D00404_0538_BCD91CANXX_TCGACGTC-CGTCTAAT_L007_R1_001.fastq.gz | fastq | 670970484.0 | 13156284.0 | E MTAB 11083:2874F23 2 210715 D00404 0538 BCD91CANXX TCGACGTC CGTCTAAT L007 | 0:51 1:0 | A:180906557;C:151329492;G:145816347;T:192872804;N:45284 | 51 | 0 | 180906557 | 151329492 | 145816347 | 192872804 | 45284 | ERX6698606 | ERS8070395 | ERA6757553 | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | 1 | 0.71682 | 0.19902 | 0.74517 | 0.52618 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | poly_a | nextera | sc | single_cell_plate | smartseq | Switzerland | 2021-12-01 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||
| 10243 | 10243 | ERR7131163 | ERX6698605 | ERS8070394 | ERP132560 | PRJEB48218 | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E-MTAB-11083 | Transcriptome Analysis | To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb. | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | F7 Nega | SAMEA10418610 | Friedrich Miescher Institute for Biomedical Research | ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418610|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F7 Nega|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry /GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 7|organism part:olfactory bulb|sample name:E MTAB 11083:F7 Nega|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E MTAB 11083:F7 Nega s | F7 Nega s | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | Experimental Factor: fraction:mCherry /GFP | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP132560 | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | 2874F19-1_210715_D00404_0538_BCD91CANXX_TCGACGTC-GTAAGGAG_L006_R1_001.fastq.gz | fastq | 652205238.0 | 12788338.0 | E MTAB 11083:2874F19 1 210715 D00404 0538 BCD91CANXX TCGACGTC GTAAGGAG L006 | 0:51 1:0 | A:173730405;C:149044598;G:145436777;T:183946928;N:46530 | 51 | 0 | 173730405 | 149044598 | 145436777 | 183946928 | 46530 | ERX6698605 | ERS8070394 | ERA6757553 | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | 1 | 0.80595 | 0.17309 | 0.71003 | 0.53696 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | poly_a | nextera | sc | single_cell_plate | smartseq | Switzerland | 2021-12-01 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||
| 10244 | 10244 | ERR7131164 | ERX6698605 | ERS8070394 | ERP132560 | PRJEB48218 | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E-MTAB-11083 | Transcriptome Analysis | To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb. | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | F7 Nega | SAMEA10418610 | Friedrich Miescher Institute for Biomedical Research | ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418610|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F7 Nega|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry /GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 7|organism part:olfactory bulb|sample name:E MTAB 11083:F7 Nega|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E MTAB 11083:F7 Nega s | F7 Nega s | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | Experimental Factor: fraction:mCherry /GFP | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP132560 | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | 2874F19-2_210715_D00404_0538_BCD91CANXX_TCGACGTC-GTAAGGAG_L007_R1_001.fastq.gz | fastq | 655593423.0 | 12854773.0 | E MTAB 11083:2874F19 2 210715 D00404 0538 BCD91CANXX TCGACGTC GTAAGGAG L007 | 0:51 1:0 | A:174715119;C:149844974;G:146250446;T:184737535;N:45349 | 51 | 0 | 174715119 | 149844974 | 146250446 | 184737535 | 45349 | ERX6698605 | ERS8070394 | ERA6757553 | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | 1 | 0.80585 | 0.17497 | 0.71078 | 0.53767 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | poly_a | nextera | sc | single_cell_plate | smartseq | Switzerland | 2021-12-01 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||
| 10245 | 10245 | ERR7131161 | ERX6698604 | ERS8070393 | ERP132560 | PRJEB48218 | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E-MTAB-11083 | Transcriptome Analysis | To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb. | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | F7 mCherry GFP | SAMEA10418609 | Friedrich Miescher Institute for Biomedical Research | ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418609|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F7 mCherry GFP|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP+|genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 7|organism part:olfactory bulb|sample name:E MTAB 11083:F7 mCherry GFP|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E MTAB 11083:F7 mCherry GFP s | F7 mCherry GFP s | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | Experimental Factor: fraction:mCherry+/GFP+ | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP132560 | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | 2874F21-1_210715_D00404_0538_BCD91CANXX_TCGACGTC-AAGGAGTA_L006_R1_001.fastq.gz | fastq | 661067151.0 | 12962101.0 | E MTAB 11083:2874F21 1 210715 D00404 0538 BCD91CANXX TCGACGTC AAGGAGTA L006 | 0:51 1:0 | A:168281419;C:157701209;G:153487197;T:181550252;N:47074 | 51 | 0 | 168281419 | 157701209 | 153487197 | 181550252 | 47074 | ERX6698604 | ERS8070393 | ERA6757553 | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | 1 | 0.39074 | 0.11592 | 0.82615 | 0.5252 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | poly_a | nextera | sc | single_cell_plate | smartseq | Switzerland | 2021-12-01 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||
| 10246 | 10246 | ERR7131162 | ERX6698604 | ERS8070393 | ERP132560 | PRJEB48218 | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E-MTAB-11083 | Transcriptome Analysis | To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb. | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | F7 mCherry GFP | SAMEA10418609 | Friedrich Miescher Institute for Biomedical Research | ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418609|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F7 mCherry GFP|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP+|genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 7|organism part:olfactory bulb|sample name:E MTAB 11083:F7 mCherry GFP|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E MTAB 11083:F7 mCherry GFP s | F7 mCherry GFP s | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | Experimental Factor: fraction:mCherry+/GFP+ | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP132560 | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | 2874F21-2_210715_D00404_0538_BCD91CANXX_TCGACGTC-AAGGAGTA_L007_R1_001.fastq.gz | fastq | 665709018.0 | 13053118.0 | E MTAB 11083:2874F21 2 210715 D00404 0538 BCD91CANXX TCGACGTC AAGGAGTA L007 | 0:51 1:0 | A:169539998;C:158884603;G:154624803;T:182614264;N:45350 | 51 | 0 | 169539998 | 158884603 | 154624803 | 182614264 | 45350 | ERX6698604 | ERS8070393 | ERA6757553 | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | 1 | 0.38966 | 0.1152 | 0.8258 | 0.53305 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | poly_a | nextera | sc | single_cell_plate | smartseq | Switzerland | 2021-12-01 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||
| 10247 | 10247 | ERR7131159 | ERX6698603 | ERS8070392 | ERP132560 | PRJEB48218 | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E-MTAB-11083 | Transcriptome Analysis | To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb. | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | F7 mCherry | SAMEA10418608 | Friedrich Miescher Institute for Biomedical Research | ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418608|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F7 mCherry|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 7|organism part:olfactory bulb|sample name:E MTAB 11083:F7 mCherry|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E MTAB 11083:F7 mCherry s | F7 mCherry s | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | Experimental Factor: fraction:mCherry+/GFP | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP132560 | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | 2874F20-1_210715_D00404_0538_BCD91CANXX_TCGACGTC-ACTGCATA_L006_R1_001.fastq.gz | fastq | 657813402.0 | 12898302.0 | E MTAB 11083:2874F20 1 210715 D00404 0538 BCD91CANXX TCGACGTC ACTGCATA L006 | 0:51 1:0 | A:175873489;C:149476129;G:143987770;T:188429588;N:46426 | 51 | 0 | 175873489 | 149476129 | 143987770 | 188429588 | 46426 | ERX6698603 | ERS8070392 | ERA6757553 | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | 1 | 0.70507 | 0.2048 | 0.75923 | 0.52828 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | poly_a | nextera | sc | single_cell_plate | smartseq | Switzerland | 2021-12-01 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||
| 10248 | 10248 | ERR7131160 | ERX6698603 | ERS8070392 | ERP132560 | PRJEB48218 | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E-MTAB-11083 | Transcriptome Analysis | To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb. | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | F7 mCherry | SAMEA10418608 | Friedrich Miescher Institute for Biomedical Research | ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418608|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F7 mCherry|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 7|organism part:olfactory bulb|sample name:E MTAB 11083:F7 mCherry|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E MTAB 11083:F7 mCherry s | F7 mCherry s | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | Experimental Factor: fraction:mCherry+/GFP | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP132560 | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | 2874F20-2_210715_D00404_0538_BCD91CANXX_TCGACGTC-ACTGCATA_L007_R1_001.fastq.gz | fastq | 660303426.0 | 12947126.0 | E MTAB 11083:2874F20 2 210715 D00404 0538 BCD91CANXX TCGACGTC ACTGCATA L007 | 0:51 1:0 | A:176607119;C:150065781;G:144613110;T:188972809;N:44607 | 51 | 0 | 176607119 | 150065781 | 144613110 | 188972809 | 44607 | ERX6698603 | ERS8070392 | ERA6757553 | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | 1 | 0.70705 | 0.20314 | 0.75852 | 0.52949 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | poly_a | nextera | sc | single_cell_plate | smartseq | Switzerland | 2021-12-01 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||
| 10249 | 10249 | ERR7131157 | ERX6698602 | ERS8070391 | ERP132560 | PRJEB48218 | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E-MTAB-11083 | Transcriptome Analysis | To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb. | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | F6 Nega | SAMEA10418607 | Friedrich Miescher Institute for Biomedical Research | ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418607|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F6 Nega|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry /GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 6|organism part:olfactory bulb|sample name:E MTAB 11083:F6 Nega|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E MTAB 11083:F6 Nega s | F6 Nega s | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | Experimental Factor: fraction:mCherry /GFP | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP132560 | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | 2874F16-1_210715_D00404_0538_BCD91CANXX_TGCAGCTA-TCTCTCCG_L006_R1_001.fastq.gz | fastq | 669627450.0 | 13129950.0 | E MTAB 11083:2874F16 1 210715 D00404 0538 BCD91CANXX TGCAGCTA TCTCTCCG L006 | 0:51 1:0 | A:177483937;C:154097051;G:148063012;T:189935690;N:47760 | 51 | 0 | 177483937 | 154097051 | 148063012 | 189935690 | 47760 | ERX6698602 | ERS8070391 | ERA6757553 | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | 1 | 0.77183 | 0.16001 | 0.71467 | 0.53352 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | poly_a | nextera | sc | single_cell_plate | smartseq | Switzerland | 2021-12-01 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||
| 10250 | 10250 | ERR7131158 | ERX6698602 | ERS8070391 | ERP132560 | PRJEB48218 | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E-MTAB-11083 | Transcriptome Analysis | To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb. | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | F6 Nega | SAMEA10418607 | Friedrich Miescher Institute for Biomedical Research | ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418607|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F6 Nega|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry /GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 6|organism part:olfactory bulb|sample name:E MTAB 11083:F6 Nega|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E MTAB 11083:F6 Nega s | F6 Nega s | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | Experimental Factor: fraction:mCherry /GFP | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP132560 | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | 2874F16-2_210715_D00404_0538_BCD91CANXX_TGCAGCTA-TCTCTCCG_L007_R1_001.fastq.gz | fastq | 671615073.0 | 13168923.0 | E MTAB 11083:2874F16 2 210715 D00404 0538 BCD91CANXX TGCAGCTA TCTCTCCG L007 | 0:51 1:0 | A:178109157;C:154619024;G:148595207;T:190246469;N:45216 | 51 | 0 | 178109157 | 154619024 | 148595207 | 190246469 | 45216 | ERX6698602 | ERS8070391 | ERA6757553 | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | 1 | 0.77284 | 0.15963 | 0.71569 | 0.53038 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | poly_a | nextera | sc | single_cell_plate | smartseq | Switzerland | 2021-12-01 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||
| 10251 | 10251 | ERR7131155 | ERX6698601 | ERS8070390 | ERP132560 | PRJEB48218 | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E-MTAB-11083 | Transcriptome Analysis | To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb. | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | F6 mCherry GFP | SAMEA10418606 | Friedrich Miescher Institute for Biomedical Research | ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418606|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F6 mCherry GFP|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP+|genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 6|organism part:olfactory bulb|sample name:E MTAB 11083:F6 mCherry GFP|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E MTAB 11083:F6 mCherry GFP s | F6 mCherry GFP s | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | Experimental Factor: fraction:mCherry+/GFP+ | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP132560 | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | 2874F18-1_210715_D00404_0538_BCD91CANXX_TCGACGTC-TATCCTCT_L006_R1_001.fastq.gz | fastq | 667663542.0 | 13091442.0 | E MTAB 11083:2874F18 1 210715 D00404 0538 BCD91CANXX TCGACGTC TATCCTCT L006 | 0:51 1:0 | A:177646483;C:152747528;G:146388226;T:190833951;N:47354 | 51 | 0 | 177646483 | 152747528 | 146388226 | 190833951 | 47354 | ERX6698601 | ERS8070390 | ERA6757553 | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | 1 | 0.67964 | 0.18123 | 0.77193 | 0.53313 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | poly_a | nextera | sc | single_cell_plate | smartseq | Switzerland | 2021-12-01 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||
| 10252 | 10252 | ERR7131156 | ERX6698601 | ERS8070390 | ERP132560 | PRJEB48218 | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E-MTAB-11083 | Transcriptome Analysis | To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb. | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | F6 mCherry GFP | SAMEA10418606 | Friedrich Miescher Institute for Biomedical Research | ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418606|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F6 mCherry GFP|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP+|genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 6|organism part:olfactory bulb|sample name:E MTAB 11083:F6 mCherry GFP|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E MTAB 11083:F6 mCherry GFP s | F6 mCherry GFP s | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | Experimental Factor: fraction:mCherry+/GFP+ | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP132560 | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | 2874F18-2_210715_D00404_0538_BCD91CANXX_TCGACGTC-TATCCTCT_L007_R1_001.fastq.gz | fastq | 670170141.0 | 13140591.0 | E MTAB 11083:2874F18 2 210715 D00404 0538 BCD91CANXX TCGACGTC TATCCTCT L007 | 0:51 1:0 | A:178425395;C:153363855;G:147033779;T:191300976;N:46136 | 51 | 0 | 178425395 | 153363855 | 147033779 | 191300976 | 46136 | ERX6698601 | ERS8070390 | ERA6757553 | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | 1 | 0.67922 | 0.18101 | 0.77141 | 0.53601 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | poly_a | nextera | sc | single_cell_plate | smartseq | Switzerland | 2021-12-01 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||
| 10253 | 10253 | ERR7131153 | ERX6698600 | ERS8070389 | ERP132560 | PRJEB48218 | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E-MTAB-11083 | Transcriptome Analysis | To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb. | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | F6 mCherry | SAMEA10418605 | Friedrich Miescher Institute for Biomedical Research | ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418605|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F6 mCherry|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 6|organism part:olfactory bulb|sample name:E MTAB 11083:F6 mCherry|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E MTAB 11083:F6 mCherry s | F6 mCherry s | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | Experimental Factor: fraction:mCherry+/GFP | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP132560 | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | 2874F17-1_210715_D00404_0538_BCD91CANXX_TCGACGTC-CTCTCTAT_L006_R1_001.fastq.gz | fastq | 677617008.0 | 13286608.0 | E MTAB 11083:2874F17 1 210715 D00404 0538 BCD91CANXX TCGACGTC CTCTCTAT L006 | 0:51 1:0 | A:181785899;C:152797687;G:147070676;T:195914808;N:47938 | 51 | 0 | 181785899 | 152797687 | 147070676 | 195914808 | 47938 | ERX6698600 | ERS8070389 | ERA6757553 | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | 1 | 0.68298 | 0.1953 | 0.76292 | 0.5395 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | poly_a | nextera | sc | single_cell_plate | smartseq | Switzerland | 2021-12-01 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||
| 10254 | 10254 | ERR7131154 | ERX6698600 | ERS8070389 | ERP132560 | PRJEB48218 | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E-MTAB-11083 | Transcriptome Analysis | To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb. | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | F6 mCherry | SAMEA10418605 | Friedrich Miescher Institute for Biomedical Research | ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418605|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F6 mCherry|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 6|organism part:olfactory bulb|sample name:E MTAB 11083:F6 mCherry|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E MTAB 11083:F6 mCherry s | F6 mCherry s | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | Experimental Factor: fraction:mCherry+/GFP | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP132560 | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | 2874F17-2_210715_D00404_0538_BCD91CANXX_TCGACGTC-CTCTCTAT_L007_R1_001.fastq.gz | fastq | 679922973.0 | 13331823.0 | E MTAB 11083:2874F17 2 210715 D00404 0538 BCD91CANXX TCGACGTC CTCTCTAT L007 | 0:51 1:0 | A:182567819;C:153380607;G:147589890;T:196337722;N:46935 | 51 | 0 | 182567819 | 153380607 | 147589890 | 196337722 | 46935 | ERX6698600 | ERS8070389 | ERA6757553 | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | 1 | 0.68391 | 0.19726 | 0.76299 | 0.54099 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | poly_a | nextera | sc | single_cell_plate | smartseq | Switzerland | 2021-12-01 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||
| 10255 | 10255 | ERR7131151 | ERX6698599 | ERS8070388 | ERP132560 | PRJEB48218 | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E-MTAB-11083 | Transcriptome Analysis | To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb. | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | F5 Nega | SAMEA10418604 | Friedrich Miescher Institute for Biomedical Research | ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418604|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F5 Nega|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry /GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 5|organism part:olfactory bulb|sample name:E MTAB 11083:F5 Nega|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E MTAB 11083:F5 Nega s | F5 Nega s | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | Experimental Factor: fraction:mCherry /GFP | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP132560 | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | 2874F13-1_210715_D00404_0538_BCD91CANXX_TGCAGCTA-AAGGAGTA_L006_R1_001.fastq.gz | fastq | 620316315.0 | 12163065.0 | E MTAB 11083:2874F13 1 210715 D00404 0538 BCD91CANXX TGCAGCTA AAGGAGTA L006 | 0:51 1:0 | A:165967355;C:141072216;G:136931219;T:176301963;N:43562 | 51 | 0 | 165967355 | 141072216 | 136931219 | 176301963 | 43562 | ERX6698599 | ERS8070388 | ERA6757553 | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | 1 | 0.78924 | 0.17457 | 0.71934 | 0.53563 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | poly_a | nextera | sc | single_cell_plate | smartseq | Switzerland | 2021-12-01 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||
| 10256 | 10256 | ERR7131152 | ERX6698599 | ERS8070388 | ERP132560 | PRJEB48218 | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E-MTAB-11083 | Transcriptome Analysis | To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb. | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | F5 Nega | SAMEA10418604 | Friedrich Miescher Institute for Biomedical Research | ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418604|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F5 Nega|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry /GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 5|organism part:olfactory bulb|sample name:E MTAB 11083:F5 Nega|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E MTAB 11083:F5 Nega s | F5 Nega s | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | Experimental Factor: fraction:mCherry /GFP | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP132560 | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | 2874F13-2_210715_D00404_0538_BCD91CANXX_TGCAGCTA-AAGGAGTA_L007_R1_001.fastq.gz | fastq | 623905338.0 | 12233438.0 | E MTAB 11083:2874F13 2 210715 D00404 0538 BCD91CANXX TGCAGCTA AAGGAGTA L007 | 0:51 1:0 | A:166996253;C:141937637;G:137825206;T:177103504;N:42738 | 51 | 0 | 166996253 | 141937637 | 137825206 | 177103504 | 42738 | ERX6698599 | ERS8070388 | ERA6757553 | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | 1 | 0.78975 | 0.17387 | 0.72153 | 0.54149 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | poly_a | nextera | sc | single_cell_plate | smartseq | Switzerland | 2021-12-01 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||
| 10257 | 10257 | ERR7131149 | ERX6698598 | ERS8070387 | ERP132560 | PRJEB48218 | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E-MTAB-11083 | Transcriptome Analysis | To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb. | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | F5 mCherry GFP | SAMEA10418603 | Friedrich Miescher Institute for Biomedical Research | ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418603|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F5 mCherry GFP|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP+|genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 5|organism part:olfactory bulb|sample name:E MTAB 11083:F5 mCherry GFP|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E MTAB 11083:F5 mCherry GFP s | F5 mCherry GFP s | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | Experimental Factor: fraction:mCherry+/GFP+ | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP132560 | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | 2874F15-1_210715_D00404_0538_BCD91CANXX_TGCAGCTA-CGTCTAAT_L006_R1_001.fastq.gz | fastq | 670154841.0 | 13140291.0 | E MTAB 11083:2874F15 1 210715 D00404 0538 BCD91CANXX TGCAGCTA CGTCTAAT L006 | 0:51 1:0 | A:182457480;C:149175456;G:144548173;T:193927470;N:46262 | 51 | 0 | 182457480 | 149175456 | 144548173 | 193927470 | 46262 | ERX6698598 | ERS8070387 | ERA6757553 | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | 1 | 0.71875 | 0.18671 | 0.76047 | 0.52398 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | poly_a | nextera | sc | single_cell_plate | smartseq | Switzerland | 2021-12-01 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||
| 10258 | 10258 | ERR7131150 | ERX6698598 | ERS8070387 | ERP132560 | PRJEB48218 | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E-MTAB-11083 | Transcriptome Analysis | To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb. | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | F5 mCherry GFP | SAMEA10418603 | Friedrich Miescher Institute for Biomedical Research | ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418603|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F5 mCherry GFP|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP+|genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 5|organism part:olfactory bulb|sample name:E MTAB 11083:F5 mCherry GFP|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E MTAB 11083:F5 mCherry GFP s | F5 mCherry GFP s | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | Experimental Factor: fraction:mCherry+/GFP+ | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP132560 | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | 2874F15-2_210715_D00404_0538_BCD91CANXX_TGCAGCTA-CGTCTAAT_L007_R1_001.fastq.gz | fastq | 674202048.0 | 13219648.0 | E MTAB 11083:2874F15 2 210715 D00404 0538 BCD91CANXX TGCAGCTA CGTCTAAT L007 | 0:51 1:0 | A:183639511;C:150168226;G:145502071;T:194847755;N:44485 | 51 | 0 | 183639511 | 150168226 | 145502071 | 194847755 | 44485 | ERX6698598 | ERS8070387 | ERA6757553 | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | 1 | 0.72111 | 0.18813 | 0.76378 | 0.5258 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | poly_a | nextera | sc | single_cell_plate | smartseq | Switzerland | 2021-12-01 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||
| 10259 | 10259 | ERR7131147 | ERX6698597 | ERS8070386 | ERP132560 | PRJEB48218 | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E-MTAB-11083 | Transcriptome Analysis | To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb. | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | F5 mCherry | SAMEA10418602 | Friedrich Miescher Institute for Biomedical Research | ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418602|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F5 mCherry|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 5|organism part:olfactory bulb|sample name:E MTAB 11083:F5 mCherry|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E MTAB 11083:F5 mCherry s | F5 mCherry s | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | Experimental Factor: fraction:mCherry+/GFP | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP132560 | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | 2874F14-1_210715_D00404_0538_BCD91CANXX_TGCAGCTA-CTAAGCCT_L006_R1_001.fastq.gz | fastq | 615458259.0 | 12067809.0 | E MTAB 11083:2874F14 1 210715 D00404 0538 BCD91CANXX TGCAGCTA CTAAGCCT L006 | 0:51 1:0 | A:165108582;C:139178883;G:134070672;T:177059757;N:40365 | 51 | 0 | 165108582 | 139178883 | 134070672 | 177059757 | 40365 | ERX6698597 | ERS8070386 | ERA6757553 | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | 1 | 0.69017 | 0.1977 | 0.77193 | 0.53352 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | poly_a | nextera | sc | single_cell_plate | smartseq | Switzerland | 2021-12-01 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||
| 10260 | 10260 | ERR7131148 | ERX6698597 | ERS8070386 | ERP132560 | PRJEB48218 | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E-MTAB-11083 | Transcriptome Analysis | To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb. | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | F5 mCherry | SAMEA10418602 | Friedrich Miescher Institute for Biomedical Research | ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418602|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F5 mCherry|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 5|organism part:olfactory bulb|sample name:E MTAB 11083:F5 mCherry|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E MTAB 11083:F5 mCherry s | F5 mCherry s | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | Experimental Factor: fraction:mCherry+/GFP | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP132560 | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | 2874F14-2_210715_D00404_0538_BCD91CANXX_TGCAGCTA-CTAAGCCT_L007_R1_001.fastq.gz | fastq | 619061613.0 | 12138463.0 | E MTAB 11083:2874F14 2 210715 D00404 0538 BCD91CANXX TGCAGCTA CTAAGCCT L007 | 0:51 1:0 | A:166167516;C:140058774;G:134972850;T:177822733;N:39740 | 51 | 0 | 166167516 | 140058774 | 134972850 | 177822733 | 39740 | ERX6698597 | ERS8070386 | ERA6757553 | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | 1 | 0.69118 | 0.20019 | 0.7707 | 0.52174 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | poly_a | nextera | sc | single_cell_plate | smartseq | Switzerland | 2021-12-01 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||
| 10261 | 10261 | ERR7131145 | ERX6698596 | ERS8070385 | ERP132560 | PRJEB48218 | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E-MTAB-11083 | Transcriptome Analysis | To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb. | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | F4 Nega | SAMEA10418601 | Friedrich Miescher Institute for Biomedical Research | ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418601|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F4 Nega|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry /GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 4|organism part:olfactory bulb|sample name:E MTAB 11083:F4 Nega|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E MTAB 11083:F4 Nega s | F4 Nega s | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | Experimental Factor: fraction:mCherry /GFP | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP132560 | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | 2874F10-1_210715_D00404_0538_BCD91CANXX_TGCAGCTA-TATCCTCT_L006_R1_001.fastq.gz | fastq | 652536687.0 | 12794837.0 | E MTAB 11083:2874F10 1 210715 D00404 0538 BCD91CANXX TGCAGCTA TATCCTCT L006 | 0:51 1:0 | A:178033231;C:144661707;G:139876661;T:189921085;N:44003 | 51 | 0 | 178033231 | 144661707 | 139876661 | 189921085 | 44003 | ERX6698596 | ERS8070385 | ERA6757553 | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | 1 | 0.76201 | 0.2508 | 0.71299 | 0.53379 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | poly_a | nextera | sc | single_cell_plate | smartseq | Switzerland | 2021-12-01 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||
| 10262 | 10262 | ERR7131146 | ERX6698596 | ERS8070385 | ERP132560 | PRJEB48218 | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E-MTAB-11083 | Transcriptome Analysis | To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb. | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | F4 Nega | SAMEA10418601 | Friedrich Miescher Institute for Biomedical Research | ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418601|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F4 Nega|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry /GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 4|organism part:olfactory bulb|sample name:E MTAB 11083:F4 Nega|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E MTAB 11083:F4 Nega s | F4 Nega s | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | Experimental Factor: fraction:mCherry /GFP | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP132560 | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | 2874F10-2_210715_D00404_0538_BCD91CANXX_TGCAGCTA-TATCCTCT_L007_R1_001.fastq.gz | fastq | 655278957.0 | 12848607.0 | E MTAB 11083:2874F10 2 210715 D00404 0538 BCD91CANXX TGCAGCTA TATCCTCT L007 | 0:51 1:0 | A:178938002;C:145332613;G:140527756;T:190437189;N:43397 | 51 | 0 | 178938002 | 145332613 | 140527756 | 190437189 | 43397 | ERX6698596 | ERS8070385 | ERA6757553 | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | 1 | 0.76188 | 0.25356 | 0.71344 | 0.53081 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | poly_a | nextera | sc | single_cell_plate | smartseq | Switzerland | 2021-12-01 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||
| 10263 | 10263 | ERR7131143 | ERX6698595 | ERS8070384 | ERP132560 | PRJEB48218 | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E-MTAB-11083 | Transcriptome Analysis | To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb. | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | F4 mCherry GFP | SAMEA10418600 | Friedrich Miescher Institute for Biomedical Research | ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418600|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F4 mCherry GFP|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP+|genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 4|organism part:olfactory bulb|sample name:E MTAB 11083:F4 mCherry GFP|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E MTAB 11083:F4 mCherry GFP s | F4 mCherry GFP s | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | Experimental Factor: fraction:mCherry+/GFP+ | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP132560 | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | 2874F12-1_210715_D00404_0538_BCD91CANXX_TGCAGCTA-ACTGCATA_L006_R1_001.fastq.gz | fastq | 297921855.0 | 5841605.0 | E MTAB 11083:2874F12 1 210715 D00404 0538 BCD91CANXX TGCAGCTA ACTGCATA L006 | 0:51 1:0 | A:82400724;C:65517496;G:64603058;T:85384480;N:16097 | 51 | 0 | 82400724 | 65517496 | 64603058 | 85384480 | 16097 | ERX6698595 | ERS8070384 | ERA6757553 | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | 1 | 0.72496 | 0.20794 | 0.81223 | 0.52529 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | poly_a | nextera | sc | single_cell_plate | smartseq | Switzerland | 2021-12-01 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||
| 10264 | 10264 | ERR7131144 | ERX6698595 | ERS8070384 | ERP132560 | PRJEB48218 | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E-MTAB-11083 | Transcriptome Analysis | To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb. | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | F4 mCherry GFP | SAMEA10418600 | Friedrich Miescher Institute for Biomedical Research | ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418600|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F4 mCherry GFP|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP+|genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 4|organism part:olfactory bulb|sample name:E MTAB 11083:F4 mCherry GFP|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E MTAB 11083:F4 mCherry GFP s | F4 mCherry GFP s | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | Experimental Factor: fraction:mCherry+/GFP+ | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP132560 | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | 2874F12-2_210715_D00404_0538_BCD91CANXX_TGCAGCTA-ACTGCATA_L007_R1_001.fastq.gz | fastq | 309412971.0 | 6066921.0 | E MTAB 11083:2874F12 2 210715 D00404 0538 BCD91CANXX TGCAGCTA ACTGCATA L007 | 0:51 1:0 | A:85531271;C:68164437;G:67129209;T:88573210;N:14844 | 51 | 0 | 85531271 | 68164437 | 67129209 | 88573210 | 14844 | ERX6698595 | ERS8070384 | ERA6757553 | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | 1 | 0.7245 | 0.20724 | 0.80468 | 0.52791 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | poly_a | nextera | sc | single_cell_plate | smartseq | Switzerland | 2021-12-01 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||
| 10265 | 10265 | ERR7131141 | ERX6698594 | ERS8070383 | ERP132560 | PRJEB48218 | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E-MTAB-11083 | Transcriptome Analysis | To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb. | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | F4 mCherry | SAMEA10418599 | Friedrich Miescher Institute for Biomedical Research | ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418599|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F4 mCherry|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 4|organism part:olfactory bulb|sample name:E MTAB 11083:F4 mCherry|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E MTAB 11083:F4 mCherry s | F4 mCherry s | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | Experimental Factor: fraction:mCherry+/GFP | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP132560 | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | 2874F11-1_210715_D00404_0538_BCD91CANXX_TGCAGCTA-GTAAGGAG_L006_R1_001.fastq.gz | fastq | 672319791.0 | 13182741.0 | E MTAB 11083:2874F11 1 210715 D00404 0538 BCD91CANXX TGCAGCTA GTAAGGAG L006 | 0:51 1:0 | A:181508034;C:150928549;G:146124085;T:193711358;N:47765 | 51 | 0 | 181508034 | 150928549 | 146124085 | 193711358 | 47765 | ERX6698594 | ERS8070383 | ERA6757553 | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | 1 | 0.71726 | 0.19842 | 0.74986 | 0.53363 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | poly_a | nextera | sc | single_cell_plate | smartseq | Switzerland | 2021-12-01 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||
| 10266 | 10266 | ERR7131142 | ERX6698594 | ERS8070383 | ERP132560 | PRJEB48218 | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E-MTAB-11083 | Transcriptome Analysis | To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb. | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | F4 mCherry | SAMEA10418599 | Friedrich Miescher Institute for Biomedical Research | ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418599|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F4 mCherry|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 4|organism part:olfactory bulb|sample name:E MTAB 11083:F4 mCherry|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E MTAB 11083:F4 mCherry s | F4 mCherry s | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | Experimental Factor: fraction:mCherry+/GFP | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP132560 | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | 2874F11-2_210715_D00404_0538_BCD91CANXX_TGCAGCTA-GTAAGGAG_L007_R1_001.fastq.gz | fastq | 675486075.0 | 13244825.0 | E MTAB 11083:2874F11 2 210715 D00404 0538 BCD91CANXX TGCAGCTA GTAAGGAG L007 | 0:51 1:0 | A:182462261;C:151688554;G:146902713;T:194385945;N:46602 | 51 | 0 | 182462261 | 151688554 | 146902713 | 194385945 | 46602 | ERX6698594 | ERS8070383 | ERA6757553 | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | 1 | 0.71745 | 0.19727 | 0.74805 | 0.53092 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | poly_a | nextera | sc | single_cell_plate | smartseq | Switzerland | 2021-12-01 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||
| 10267 | 10267 | ERR7131139 | ERX6698593 | ERS8070382 | ERP132560 | PRJEB48218 | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E-MTAB-11083 | Transcriptome Analysis | To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb. | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | F3 Nega | SAMEA10418598 | Friedrich Miescher Institute for Biomedical Research | ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418598|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F3 Nega|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry /GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 3|organism part:olfactory bulb|sample name:E MTAB 11083:F3 Nega|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E MTAB 11083:F3 Nega s | F3 Nega s | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | Experimental Factor: fraction:mCherry /GFP | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP132560 | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | 2874F7-1_210715_D00404_0538_BCD91CANXX_CGATCAGT-CGTCTAAT_L006_R1_001.fastq.gz | fastq | 630093627.0 | 12354777.0 | E MTAB 11083:2874F7 1 210715 D00404 0538 BCD91CANXX CGATCAGT CGTCTAAT L006 | 0:51 1:0 | A:165132739;C:146179836;G:142796880;T:175940613;N:43559 | 51 | 0 | 165132739 | 146179836 | 142796880 | 175940613 | 43559 | ERX6698593 | ERS8070382 | ERA6757553 | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | 1 | 0.81672 | 0.15219 | 0.71277 | 0.52461 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | poly_a | nextera | sc | single_cell_plate | smartseq | Switzerland | 2021-12-01 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||
| 10268 | 10268 | ERR7131140 | ERX6698593 | ERS8070382 | ERP132560 | PRJEB48218 | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E-MTAB-11083 | Transcriptome Analysis | To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb. | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | F3 Nega | SAMEA10418598 | Friedrich Miescher Institute for Biomedical Research | ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418598|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F3 Nega|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry /GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 3|organism part:olfactory bulb|sample name:E MTAB 11083:F3 Nega|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E MTAB 11083:F3 Nega s | F3 Nega s | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | Experimental Factor: fraction:mCherry /GFP | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP132560 | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | 2874F7-2_210715_D00404_0538_BCD91CANXX_CGATCAGT-CGTCTAAT_L007_R1_001.fastq.gz | fastq | 632305242.0 | 12398142.0 | E MTAB 11083:2874F7 2 210715 D00404 0538 BCD91CANXX CGATCAGT CGTCTAAT L007 | 0:51 1:0 | A:165832174;C:146751895;G:143384539;T:176293313;N:43321 | 51 | 0 | 165832174 | 146751895 | 143384539 | 176293313 | 43321 | ERX6698593 | ERS8070382 | ERA6757553 | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | 1 | 0.81791 | 0.15444 | 0.7151 | 0.52585 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | poly_a | nextera | sc | single_cell_plate | smartseq | Switzerland | 2021-12-01 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||
| 10269 | 10269 | ERR7131137 | ERX6698592 | ERS8070381 | ERP132560 | PRJEB48218 | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E-MTAB-11083 | Transcriptome Analysis | To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb. | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | F3 mCherry GFP | SAMEA10418597 | Friedrich Miescher Institute for Biomedical Research | ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418597|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F3 mCherry GFP|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP+|genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 3|organism part:olfactory bulb|sample name:E MTAB 11083:F3 mCherry GFP|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E MTAB 11083:F3 mCherry GFP s | F3 mCherry GFP s | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | Experimental Factor: fraction:mCherry+/GFP+ | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP132560 | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | 2874F9-1_210715_D00404_0538_BCD91CANXX_TGCAGCTA-CTCTCTAT_L006_R1_001.fastq.gz | fastq | 660379620.0 | 12948620.0 | E MTAB 11083:2874F9 1 210715 D00404 0538 BCD91CANXX TGCAGCTA CTCTCTAT L006 | 0:51 1:0 | A:178482695;C:147417787;G:143797476;T:190635173;N:46489 | 51 | 0 | 178482695 | 147417787 | 143797476 | 190635173 | 46489 | ERX6698592 | ERS8070381 | ERA6757553 | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | 1 | 0.65635 | 0.17778 | 0.76899 | 0.52957 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | poly_a | nextera | sc | single_cell_plate | smartseq | Switzerland | 2021-12-01 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||
| 10270 | 10270 | ERR7131138 | ERX6698592 | ERS8070381 | ERP132560 | PRJEB48218 | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E-MTAB-11083 | Transcriptome Analysis | To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb. | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | F3 mCherry GFP | SAMEA10418597 | Friedrich Miescher Institute for Biomedical Research | ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418597|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F3 mCherry GFP|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP+|genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 3|organism part:olfactory bulb|sample name:E MTAB 11083:F3 mCherry GFP|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E MTAB 11083:F3 mCherry GFP s | F3 mCherry GFP s | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | Experimental Factor: fraction:mCherry+/GFP+ | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP132560 | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | 2874F9-2_210715_D00404_0538_BCD91CANXX_TGCAGCTA-CTCTCTAT_L007_R1_001.fastq.gz | fastq | 662411307.0 | 12988457.0 | E MTAB 11083:2874F9 2 210715 D00404 0538 BCD91CANXX TGCAGCTA CTCTCTAT L007 | 0:51 1:0 | A:179158323;C:147942300;G:144319925;T:190945370;N:45389 | 51 | 0 | 179158323 | 147942300 | 144319925 | 190945370 | 45389 | ERX6698592 | ERS8070381 | ERA6757553 | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | 1 | 0.65813 | 0.17896 | 0.77076 | 0.52835 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | poly_a | nextera | sc | single_cell_plate | smartseq | Switzerland | 2021-12-01 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||
| 10271 | 10271 | ERR7131135 | ERX6698591 | ERS8070380 | ERP132560 | PRJEB48218 | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E-MTAB-11083 | Transcriptome Analysis | To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb. | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | F3 mCherry | SAMEA10418596 | Friedrich Miescher Institute for Biomedical Research | ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418596|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F3 mCherry|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 3|organism part:olfactory bulb|sample name:E MTAB 11083:F3 mCherry|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E MTAB 11083:F3 mCherry s | F3 mCherry s | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | Experimental Factor: fraction:mCherry+/GFP | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP132560 | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | 2874F8-1_210715_D00404_0538_BCD91CANXX_CGATCAGT-TCTCTCCG_L006_R1_001.fastq.gz | fastq | 698608353.0 | 13698203.0 | E MTAB 11083:2874F8 1 210715 D00404 0538 BCD91CANXX CGATCAGT TCTCTCCG L006 | 0:51 1:0 | A:188718612;C:156814081;G:151125083;T:201901570;N:49007 | 51 | 0 | 188718612 | 156814081 | 151125083 | 201901570 | 49007 | ERX6698591 | ERS8070380 | ERA6757553 | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | 1 | 0.70185 | 0.21547 | 0.75588 | 0.53853 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | poly_a | nextera | sc | single_cell_plate | smartseq | Switzerland | 2021-12-01 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||
| 10272 | 10272 | ERR7131136 | ERX6698591 | ERS8070380 | ERP132560 | PRJEB48218 | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E-MTAB-11083 | Transcriptome Analysis | To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb. | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | F3 mCherry | SAMEA10418596 | Friedrich Miescher Institute for Biomedical Research | ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418596|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F3 mCherry|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 3|organism part:olfactory bulb|sample name:E MTAB 11083:F3 mCherry|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E MTAB 11083:F3 mCherry s | F3 mCherry s | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | Experimental Factor: fraction:mCherry+/GFP | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP132560 | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | 2874F8-2_210715_D00404_0538_BCD91CANXX_CGATCAGT-TCTCTCCG_L007_R1_001.fastq.gz | fastq | 699869175.0 | 13722925.0 | E MTAB 11083:2874F8 2 210715 D00404 0538 BCD91CANXX CGATCAGT TCTCTCCG L007 | 0:51 1:0 | A:189192315;C:157176897;G:151498983;T:201952867;N:48113 | 51 | 0 | 189192315 | 157176897 | 151498983 | 201952867 | 48113 | ERX6698591 | ERS8070380 | ERA6757553 | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | 1 | 0.70221 | 0.21684 | 0.75621 | 0.53787 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | poly_a | nextera | sc | single_cell_plate | smartseq | Switzerland | 2021-12-01 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||
| 10273 | 10273 | ERR7131133 | ERX6698590 | ERS8070379 | ERP132560 | PRJEB48218 | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E-MTAB-11083 | Transcriptome Analysis | To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb. | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | F2 Nega | SAMEA10418595 | Friedrich Miescher Institute for Biomedical Research | ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418595|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F2 Nega|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry /GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 2|organism part:olfactory bulb|sample name:E MTAB 11083:F2 Nega|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E MTAB 11083:F2 Nega s | F2 Nega s | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | Experimental Factor: fraction:mCherry /GFP | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP132560 | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | 2874F4-1_210715_D00404_0538_BCD91CANXX_CGATCAGT-ACTGCATA_L006_R1_001.fastq.gz | fastq | 573812985.0 | 11251235.0 | E MTAB 11083:2874F4 1 210715 D00404 0538 BCD91CANXX CGATCAGT ACTGCATA L006 | 0:51 1:0 | A:150716404;C:132203167;G:129732968;T:161120057;N:40389 | 51 | 0 | 150716404 | 132203167 | 129732968 | 161120057 | 40389 | ERX6698590 | ERS8070379 | ERA6757553 | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | 1 | 0.81899 | 0.17302 | 0.72281 | 0.54315 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | poly_a | nextera | sc | single_cell_plate | smartseq | Switzerland | 2021-12-01 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||
| 10274 | 10274 | ERR7131134 | ERX6698590 | ERS8070379 | ERP132560 | PRJEB48218 | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E-MTAB-11083 | Transcriptome Analysis | To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb. | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | F2 Nega | SAMEA10418595 | Friedrich Miescher Institute for Biomedical Research | ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418595|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F2 Nega|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry /GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 2|organism part:olfactory bulb|sample name:E MTAB 11083:F2 Nega|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E MTAB 11083:F2 Nega s | F2 Nega s | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | Experimental Factor: fraction:mCherry /GFP | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP132560 | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | 2874F4-2_210715_D00404_0538_BCD91CANXX_CGATCAGT-ACTGCATA_L007_R1_001.fastq.gz | fastq | 576184587.0 | 11297737.0 | E MTAB 11083:2874F4 2 210715 D00404 0538 BCD91CANXX CGATCAGT ACTGCATA L007 | 0:51 1:0 | A:151387408;C:132788210;G:130392043;T:161577529;N:39397 | 51 | 0 | 151387408 | 132788210 | 130392043 | 161577529 | 39397 | ERX6698590 | ERS8070379 | ERA6757553 | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | 1 | 0.81999 | 0.17082 | 0.72196 | 0.54505 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | poly_a | nextera | sc | single_cell_plate | smartseq | Switzerland | 2021-12-01 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||
| 10275 | 10275 | ERR7131131 | ERX6698589 | ERS8070378 | ERP132560 | PRJEB48218 | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E-MTAB-11083 | Transcriptome Analysis | To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb. | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | F2 mCherry GFP | SAMEA10418594 | Friedrich Miescher Institute for Biomedical Research | ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418594|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F2 mCherry GFP|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP+|genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 2|organism part:olfactory bulb|sample name:E MTAB 11083:F2 mCherry GFP|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E MTAB 11083:F2 mCherry GFP s | F2 mCherry GFP s | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | Experimental Factor: fraction:mCherry+/GFP+ | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP132560 | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | 2874F6-1_210715_D00404_0538_BCD91CANXX_CGATCAGT-CTAAGCCT_L006_R1_001.fastq.gz | fastq | 654811032.0 | 12839432.0 | E MTAB 11083:2874F6 1 210715 D00404 0538 BCD91CANXX CGATCAGT CTAAGCCT L006 | 0:51 1:0 | A:177403486;C:146202733;G:142207545;T:188951373;N:45895 | 51 | 0 | 177403486 | 146202733 | 142207545 | 188951373 | 45895 | ERX6698589 | ERS8070378 | ERA6757553 | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | 1 | 0.67854 | 0.21407 | 0.76104 | 0.52713 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | poly_a | nextera | sc | single_cell_plate | smartseq | Switzerland | 2021-12-01 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||
| 10276 | 10276 | ERR7131132 | ERX6698589 | ERS8070378 | ERP132560 | PRJEB48218 | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E-MTAB-11083 | Transcriptome Analysis | To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb. | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | F2 mCherry GFP | SAMEA10418594 | Friedrich Miescher Institute for Biomedical Research | ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418594|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F2 mCherry GFP|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP+|genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 2|organism part:olfactory bulb|sample name:E MTAB 11083:F2 mCherry GFP|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E MTAB 11083:F2 mCherry GFP s | F2 mCherry GFP s | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | Experimental Factor: fraction:mCherry+/GFP+ | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP132560 | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | 2874F6-2_210715_D00404_0538_BCD91CANXX_CGATCAGT-CTAAGCCT_L007_R1_001.fastq.gz | fastq | 656911926.0 | 12880626.0 | E MTAB 11083:2874F6 2 210715 D00404 0538 BCD91CANXX CGATCAGT CTAAGCCT L007 | 0:51 1:0 | A:178119212;C:146730132;G:142725348;T:189293212;N:44022 | 51 | 0 | 178119212 | 146730132 | 142725348 | 189293212 | 44022 | ERX6698589 | ERS8070378 | ERA6757553 | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | 1 | 0.68162 | 0.21431 | 0.761 | 0.5071 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | poly_a | nextera | sc | single_cell_plate | smartseq | Switzerland | 2021-12-01 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||
| 10277 | 10277 | ERR7131129 | ERX6698588 | ERS8070377 | ERP132560 | PRJEB48218 | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E-MTAB-11083 | Transcriptome Analysis | To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb. | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | F2 mCherry | SAMEA10418593 | Friedrich Miescher Institute for Biomedical Research | ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418593|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F2 mCherry|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 2|organism part:olfactory bulb|sample name:E MTAB 11083:F2 mCherry|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E MTAB 11083:F2 mCherry s | F2 mCherry s | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | Experimental Factor: fraction:mCherry+/GFP | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP132560 | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | 2874F5-1_210715_D00404_0538_BCD91CANXX_CGATCAGT-AAGGAGTA_L006_R1_001.fastq.gz | fastq | 607851558.0 | 11918658.0 | E MTAB 11083:2874F5 1 210715 D00404 0538 BCD91CANXX CGATCAGT AAGGAGTA L006 | 0:51 1:0 | A:164814138;C:136071648;G:132945950;T:173976881;N:42941 | 51 | 0 | 164814138 | 136071648 | 132945950 | 173976881 | 42941 | ERX6698588 | ERS8070377 | ERA6757553 | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | 1 | 0.78967 | 0.18905 | 0.73241 | 0.52347 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | poly_a | nextera | sc | single_cell_plate | smartseq | Switzerland | 2021-12-01 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||
| 10278 | 10278 | ERR7131130 | ERX6698588 | ERS8070377 | ERP132560 | PRJEB48218 | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E-MTAB-11083 | Transcriptome Analysis | To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb. | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | F2 mCherry | SAMEA10418593 | Friedrich Miescher Institute for Biomedical Research | ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418593|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F2 mCherry|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 2|organism part:olfactory bulb|sample name:E MTAB 11083:F2 mCherry|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E MTAB 11083:F2 mCherry s | F2 mCherry s | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | Experimental Factor: fraction:mCherry+/GFP | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP132560 | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | 2874F5-2_210715_D00404_0538_BCD91CANXX_CGATCAGT-AAGGAGTA_L007_R1_001.fastq.gz | fastq | 610931805.0 | 11979055.0 | E MTAB 11083:2874F5 2 210715 D00404 0538 BCD91CANXX CGATCAGT AAGGAGTA L007 | 0:51 1:0 | A:165714775;C:136822925;G:133684294;T:174667814;N:41997 | 51 | 0 | 165714775 | 136822925 | 133684294 | 174667814 | 41997 | ERX6698588 | ERS8070377 | ERA6757553 | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | 1 | 0.78869 | 0.18885 | 0.73156 | 0.51691 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | poly_a | nextera | sc | single_cell_plate | smartseq | Switzerland | 2021-12-01 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||
| 10279 | 10279 | ERR7131127 | ERX6698587 | ERS8070376 | ERP132560 | PRJEB48218 | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E-MTAB-11083 | Transcriptome Analysis | To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb. | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | F1 Nega | SAMEA10418592 | Friedrich Miescher Institute for Biomedical Research | ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418592|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F1 Nega|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry /GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 1|organism part:olfactory bulb|sample name:E MTAB 11083:F1 Nega|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E MTAB 11083:F1 Nega s | F1 Nega s | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | Experimental Factor: fraction:mCherry /GFP | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP132560 | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | 2874F1-1_210715_D00404_0538_BCD91CANXX_CGATCAGT-CTCTCTAT_L006_R1_001.fastq.gz | fastq | 619561872.0 | 12148272.0 | E MTAB 11083:2874F1 1 210715 D00404 0538 BCD91CANXX CGATCAGT CTCTCTAT L006 | 0:51 1:0 | A:164385545;C:141746313;G:138163219;T:175223323;N:43472 | 51 | 0 | 164385545 | 141746313 | 138163219 | 175223323 | 43472 | ERX6698587 | ERS8070376 | ERA6757553 | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | 1 | 0.80258 | 0.17703 | 0.72614 | 0.53483 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | poly_a | nextera | sc | single_cell_plate | smartseq | Switzerland | 2021-12-01 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||
| 10280 | 10280 | ERR7131128 | ERX6698587 | ERS8070376 | ERP132560 | PRJEB48218 | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E-MTAB-11083 | Transcriptome Analysis | To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb. | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | F1 Nega | SAMEA10418592 | Friedrich Miescher Institute for Biomedical Research | ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418592|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F1 Nega|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry /GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 1|organism part:olfactory bulb|sample name:E MTAB 11083:F1 Nega|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E MTAB 11083:F1 Nega s | F1 Nega s | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | Experimental Factor: fraction:mCherry /GFP | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP132560 | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | 2874F1-2_210715_D00404_0538_BCD91CANXX_CGATCAGT-CTCTCTAT_L007_R1_001.fastq.gz | fastq | 621481971.0 | 12185921.0 | E MTAB 11083:2874F1 2 210715 D00404 0538 BCD91CANXX CGATCAGT CTCTCTAT L007 | 0:51 1:0 | A:165030774;C:142231469;G:138651078;T:175527080;N:41570 | 51 | 0 | 165030774 | 142231469 | 138651078 | 175527080 | 41570 | ERX6698587 | ERS8070376 | ERA6757553 | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | 1 | 0.80248 | 0.17699 | 0.726 | 0.53711 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | poly_a | nextera | sc | single_cell_plate | smartseq | Switzerland | 2021-12-01 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||
| 10281 | 10281 | ERR7131125 | ERX6698586 | ERS8070375 | ERP132560 | PRJEB48218 | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E-MTAB-11083 | Transcriptome Analysis | To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb. | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | F1 mCherry GFP | SAMEA10418591 | Friedrich Miescher Institute for Biomedical Research | ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418591|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F1 mCherry GFP|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP+|genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 1|organism part:olfactory bulb|sample name:E MTAB 11083:F1 mCherry GFP|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E MTAB 11083:F1 mCherry GFP s | F1 mCherry GFP s | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | Experimental Factor: fraction:mCherry+/GFP+ | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP132560 | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | 2874F3-1_210715_D00404_0538_BCD91CANXX_CGATCAGT-GTAAGGAG_L006_R1_001.fastq.gz | fastq | 651189369.0 | 12768419.0 | E MTAB 11083:2874F3 1 210715 D00404 0538 BCD91CANXX CGATCAGT GTAAGGAG L006 | 0:51 1:0 | A:176398415;C:145659111;G:142137610;T:186948280;N:45953 | 51 | 0 | 176398415 | 145659111 | 142137610 | 186948280 | 45953 | ERX6698586 | ERS8070375 | ERA6757553 | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | 1 | 0.69561 | 0.18489 | 0.75345 | 0.52316 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | poly_a | nextera | sc | single_cell_plate | smartseq | Switzerland | 2021-12-01 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||
| 10282 | 10282 | ERR7131126 | ERX6698586 | ERS8070375 | ERP132560 | PRJEB48218 | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E-MTAB-11083 | Transcriptome Analysis | To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb. | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | F1 mCherry GFP | SAMEA10418591 | Friedrich Miescher Institute for Biomedical Research | ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418591|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F1 mCherry GFP|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP+|genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 1|organism part:olfactory bulb|sample name:E MTAB 11083:F1 mCherry GFP|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E MTAB 11083:F1 mCherry GFP s | F1 mCherry GFP s | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | Experimental Factor: fraction:mCherry+/GFP+ | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP132560 | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | 2874F3-2_210715_D00404_0538_BCD91CANXX_CGATCAGT-GTAAGGAG_L007_R1_001.fastq.gz | fastq | 653398944.0 | 12811744.0 | E MTAB 11083:2874F3 2 210715 D00404 0538 BCD91CANXX CGATCAGT GTAAGGAG L007 | 0:51 1:0 | A:177139734;C:146171766;G:142707146;T:187335475;N:44823 | 51 | 0 | 177139734 | 146171766 | 142707146 | 187335475 | 44823 | ERX6698586 | ERS8070375 | ERA6757553 | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | 1 | 0.69496 | 0.18414 | 0.75375 | 0.52317 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | poly_a | nextera | sc | single_cell_plate | smartseq | Switzerland | 2021-12-01 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||
| 10283 | 10283 | ERR7131123 | ERX6698585 | ERS8070374 | ERP132560 | PRJEB48218 | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E-MTAB-11083 | Transcriptome Analysis | To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb. | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | F1 mCherry | SAMEA10418590 | Friedrich Miescher Institute for Biomedical Research | ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418590|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F1 mCherry|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 1|organism part:olfactory bulb|sample name:E MTAB 11083:F1 mCherry|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E MTAB 11083:F1 mCherry s | F1 mCherry s | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | Experimental Factor: fraction:mCherry+/GFP | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP132560 | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | 2874F2-1_210715_D00404_0538_BCD91CANXX_CGATCAGT-TATCCTCT_L006_R1_001.fastq.gz | fastq | 613574319.0 | 12030869.0 | E MTAB 11083:2874F2 1 210715 D00404 0538 BCD91CANXX CGATCAGT TATCCTCT L006 | 0:51 1:0 | A:164891629;C:138401759;G:135818073;T:174419828;N:43030 | 51 | 0 | 164891629 | 138401759 | 135818073 | 174419828 | 43030 | ERX6698585 | ERS8070374 | ERA6757553 | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | 1 | 0.79499 | 0.21674 | 0.73359 | 0.52207 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | poly_a | nextera | sc | single_cell_plate | smartseq | Switzerland | 2021-12-01 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||
| 10284 | 10284 | ERR7131124 | ERX6698585 | ERS8070374 | ERP132560 | PRJEB48218 | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E-MTAB-11083 | Transcriptome Analysis | To investigate the effects of rabies infection on neuronal gene expression we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb. | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | F1 mCherry | SAMEA10418590 | Friedrich Miescher Institute for Biomedical Research | ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418590|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F1 mCherry|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP |genotype:Tg[gad1b:Gal4 UAS:TVA mCherry]|individual:pool 1|organism part:olfactory bulb|sample name:E MTAB 11083:F1 mCherry|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | E MTAB 11083:F1 mCherry s | F1 mCherry s | Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4 UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al Nature protocol 2014 with the following modifications: For cDNA pre amplification up to 10ng of RNA was used as input typically 1 3ng and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific 50C for 10min 80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation using in house purified Tn5 Picelli et al Genome Research 2014 and Illumina Nextera primers. | Experimental Factor: fraction:mCherry+/GFP | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP132560 | Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb | ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07 | 2874F2-2_210715_D00404_0538_BCD91CANXX_CGATCAGT-TATCCTCT_L007_R1_001.fastq.gz | fastq | 615666798.0 | 12071898.0 | E MTAB 11083:2874F2 2 210715 D00404 0538 BCD91CANXX CGATCAGT TATCCTCT L007 | 0:51 1:0 | A:165536502;C:138952758;G:136375193;T:174760659;N:41686 | 51 | 0 | 165536502 | 138952758 | 136375193 | 174760659 | 41686 | ERX6698585 | ERS8070374 | ERA6757553 | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive | 1 | 0.7941 | 0.21645 | 0.73494 | 0.50761 | 51 | B | usable mapping rate | illumina | hiseq_era | unknown | poly_a | nextera | sc | single_cell_plate | smartseq | Switzerland | 2021-12-01 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||
| 57271 | 57271 | SRR12577970 | SRX9064853 | SRS7314026 | SRP279881 | PRJNA612371 | Danio rerio strain:TU Raw sequence reads | PRJNA612371 | Whole Genome Sequencing | Hemorrhage stroke is a severe vascular disease of the brain with a high mortality rate in humans. Sodium tanshinone IIA sulfonate STS is a water soluble derivative of tanshinone IIA which is the main active ingredient of Salvia miltiorrhiza Bge known as Danshen in Chinese and has been approved as a commercial drug for treating cardiovascular disease by the China Food and Drug Administration. In our previous study we established a HMG COA inhibitor atorvastatin Ator induced zebrafish model of cerebral hemorrhage and found that STS dramatically decreased both the hemorrhage rate and hemorrhage area although the underlying mechanism was not fully elucidated. Therefore in the present study we conducted transcriptome analysis of the protective effect of STS against Ator induced cerebral hemorrhage in zebrafish using RNA Seq technology and further clarify its underlying molecular mechanisms on HIF 1 and its regulators i.e. the PI3K/Akt and MAPK signaling pathways were verified by real time PCR analysis and specific pharmacological inhibitors. We are also able to show that hemoglobin carbonic anhydrase Na+/H+ exchanger and HIF 1 genes might be potential biomarkers of Ator induced cerebral hemorrhage in zebrafish as well as pharmacological targets of STS. This study also provided evidence of bio markers involved in hemorrhage stroke and improved understanding of the effects of HMG COA inhibition on vascular permeability and cerebral hemorrhage. | Ctrl 3 zebrafish | strain:TU|isolate:not collected|breed:not collected|cultivar:not collected|ecotype:not collected|age:2dpf|dev stage:not collected|sex:not applicable|tissue:head|geo loc name:China:Shanghai|sample type:model organism|replicate:replicate=biological replicate 6'|BioSampleModel:Model organism or animal | Ctrl 3 zebrafish | Ctrl 3 zebrafish | Ctrl 3 zebrafish | transcriptome | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | BGISEQ | BGISEQ-500 | SRP279881 | Ctrl_3_1.fq.gz | fastq | 1061227250.0 | 21224545.0 | Ctrl 3 1.fq.gz | 0:50 | A:286099732;C:241244619;G:246876865;T:287006034;N:0 | 50 | 286099732 | 241244619 | 246876865 | 287006034 | 0 | SRX9064853 | SRS7314026 | SRA1120721 | Shanghai University of Traditional Chinese Medicine|Longhua Hospital | Shanghai University of Traditional Chinese Medicine | 1 | 0.94563 | 0.10453 | 0.70552 | 0.46655 | 50 | B | usable mapping rate | bgi | bgi | unknown | poly_a | unknown | bulk | unknown | unknown | China | 2020-09-03 | Hatching | Embryo | Head | Nervous System | ||||||||||||||||||||||||||||
| 57272 | 57272 | SRR12577971 | SRX9064852 | SRS7314025 | SRP279881 | PRJNA612371 | Danio rerio strain:TU Raw sequence reads | PRJNA612371 | Whole Genome Sequencing | Hemorrhage stroke is a severe vascular disease of the brain with a high mortality rate in humans. Sodium tanshinone IIA sulfonate STS is a water soluble derivative of tanshinone IIA which is the main active ingredient of Salvia miltiorrhiza Bge known as Danshen in Chinese and has been approved as a commercial drug for treating cardiovascular disease by the China Food and Drug Administration. In our previous study we established a HMG COA inhibitor atorvastatin Ator induced zebrafish model of cerebral hemorrhage and found that STS dramatically decreased both the hemorrhage rate and hemorrhage area although the underlying mechanism was not fully elucidated. Therefore in the present study we conducted transcriptome analysis of the protective effect of STS against Ator induced cerebral hemorrhage in zebrafish using RNA Seq technology and further clarify its underlying molecular mechanisms on HIF 1 and its regulators i.e. the PI3K/Akt and MAPK signaling pathways were verified by real time PCR analysis and specific pharmacological inhibitors. We are also able to show that hemoglobin carbonic anhydrase Na+/H+ exchanger and HIF 1 genes might be potential biomarkers of Ator induced cerebral hemorrhage in zebrafish as well as pharmacological targets of STS. This study also provided evidence of bio markers involved in hemorrhage stroke and improved understanding of the effects of HMG COA inhibition on vascular permeability and cerebral hemorrhage. | Ctrl 2 zebrafish | strain:TU|isolate:not collected|breed:not collected|cultivar:not collected|ecotype:not collected|age:2dpf|dev stage:not collected|sex:not applicable|tissue:head|geo loc name:China:Shanghai|sample type:model organism|replicate:replicate=biological replicate 5 prime|BioSampleModel:Model organism or animal | Ctrl 2 zebrafish | Ctrl 2 zebrafish | Ctrl 2 zebrafish | transcriptome | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | BGISEQ | BGISEQ-500 | SRP279881 | Ctrl_2_1.fq.gz | fastq | 1058116700.0 | 21162334.0 | Ctrl 2 1.fq.gz | 0:50 | A:287223905;C:238707750;G:244987559;T:287197486;N:0 | 50 | 287223905 | 238707750 | 244987559 | 287197486 | 0 | SRX9064852 | SRS7314025 | SRA1120721 | Shanghai University of Traditional Chinese Medicine|Longhua Hospital | Shanghai University of Traditional Chinese Medicine | 1 | 0.94534 | 0.11174 | 0.70104 | 0.47946 | 50 | B | usable mapping rate | bgi | bgi | unknown | poly_a | unknown | bulk | unknown | unknown | China | 2020-09-03 | Hatching | Embryo | Head | Nervous System | ||||||||||||||||||||||||||||
| 57273 | 57273 | SRR12577972 | SRX9064851 | SRS7314024 | SRP279881 | PRJNA612371 | Danio rerio strain:TU Raw sequence reads | PRJNA612371 | Whole Genome Sequencing | Hemorrhage stroke is a severe vascular disease of the brain with a high mortality rate in humans. Sodium tanshinone IIA sulfonate STS is a water soluble derivative of tanshinone IIA which is the main active ingredient of Salvia miltiorrhiza Bge known as Danshen in Chinese and has been approved as a commercial drug for treating cardiovascular disease by the China Food and Drug Administration. In our previous study we established a HMG COA inhibitor atorvastatin Ator induced zebrafish model of cerebral hemorrhage and found that STS dramatically decreased both the hemorrhage rate and hemorrhage area although the underlying mechanism was not fully elucidated. Therefore in the present study we conducted transcriptome analysis of the protective effect of STS against Ator induced cerebral hemorrhage in zebrafish using RNA Seq technology and further clarify its underlying molecular mechanisms on HIF 1 and its regulators i.e. the PI3K/Akt and MAPK signaling pathways were verified by real time PCR analysis and specific pharmacological inhibitors. We are also able to show that hemoglobin carbonic anhydrase Na+/H+ exchanger and HIF 1 genes might be potential biomarkers of Ator induced cerebral hemorrhage in zebrafish as well as pharmacological targets of STS. This study also provided evidence of bio markers involved in hemorrhage stroke and improved understanding of the effects of HMG COA inhibition on vascular permeability and cerebral hemorrhage. | Ctrl 1 zebrafish | strain:TU|isolate:not collected|breed:not collected|cultivar:not collected|ecotype:not collected|age:2dpf|dev stage:not collected|sex:not applicable|tissue:head|geo loc name:China:Shanghai|sample type:model organism|replicate:replicate=biological replicate 4'|BioSampleModel:Model organism or animal | Ctrl 1 zebrafish | Ctrl 1 zebrafish | Ctrl 1 zebrafish | transcriptome | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | BGISEQ | BGISEQ-500 | SRP279881 | Ctrl_1_1.fq.gz | fastq | 1065919600.0 | 21318392.0 | Ctrl 1 1.fq.gz | 0:50 | A:295920720;C:242146677;G:242962633;T:284889570;N:0 | 50 | 295920720 | 242146677 | 242962633 | 284889570 | 0 | SRX9064851 | SRS7314024 | SRA1120721 | Shanghai University of Traditional Chinese Medicine|Longhua Hospital | Shanghai University of Traditional Chinese Medicine | 1 | 0.94228 | 0.15007 | 0.68028 | 0.46845 | 50 | B | usable mapping rate | bgi | bgi | unknown | poly_a | unknown | bulk | unknown | unknown | China | 2020-09-03 | Hatching | Embryo | Head | Nervous System | ||||||||||||||||||||||||||||
| 57274 | 57274 | SRR12577973 | SRX9064850 | SRS7314023 | SRP279881 | PRJNA612371 | Danio rerio strain:TU Raw sequence reads | PRJNA612371 | Whole Genome Sequencing | Hemorrhage stroke is a severe vascular disease of the brain with a high mortality rate in humans. Sodium tanshinone IIA sulfonate STS is a water soluble derivative of tanshinone IIA which is the main active ingredient of Salvia miltiorrhiza Bge known as Danshen in Chinese and has been approved as a commercial drug for treating cardiovascular disease by the China Food and Drug Administration. In our previous study we established a HMG COA inhibitor atorvastatin Ator induced zebrafish model of cerebral hemorrhage and found that STS dramatically decreased both the hemorrhage rate and hemorrhage area although the underlying mechanism was not fully elucidated. Therefore in the present study we conducted transcriptome analysis of the protective effect of STS against Ator induced cerebral hemorrhage in zebrafish using RNA Seq technology and further clarify its underlying molecular mechanisms on HIF 1 and its regulators i.e. the PI3K/Akt and MAPK signaling pathways were verified by real time PCR analysis and specific pharmacological inhibitors. We are also able to show that hemoglobin carbonic anhydrase Na+/H+ exchanger and HIF 1 genes might be potential biomarkers of Ator induced cerebral hemorrhage in zebrafish as well as pharmacological targets of STS. This study also provided evidence of bio markers involved in hemorrhage stroke and improved understanding of the effects of HMG COA inhibition on vascular permeability and cerebral hemorrhage. | Ator STS 3 zebrafish | strain:TU|isolate:not collected|breed:not collected|cultivar:not collected|ecotype:not collected|age:2dpf|dev stage:not collected|sex:not applicable|tissue:head|geo loc name:China:Shanghai|sample type:model organism|replicate:replicate=biological replicate 3 prime|BioSampleModel:Model organism or animal | Ator STS 3 zebrafish | Ator STS 3 zebrafish | Ator STS 3 zebrafish | transcriptome | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | BGISEQ | BGISEQ-500 | SRP279881 | Ator_STS_3_1.fq.gz | fastq | 1059717350.0 | 21194347.0 | Ator STS 3 1.fq.gz | 0:50 | A:287279590;C:239708340;G:245740313;T:286989107;N:0 | 50 | 287279590 | 239708340 | 245740313 | 286989107 | 0 | SRX9064850 | SRS7314023 | SRA1120721 | Shanghai University of Traditional Chinese Medicine|Longhua Hospital | Shanghai University of Traditional Chinese Medicine | 1 | 0.94345 | 0.10458 | 0.69686 | 0.4755 | 50 | B | usable mapping rate | bgi | bgi | unknown | poly_a | unknown | bulk | unknown | unknown | China | 2020-09-03 | Hatching | Embryo | Head | Nervous System | ||||||||||||||||||||||||||||
| 57275 | 57275 | SRR12577974 | SRX9064849 | SRS7314022 | SRP279881 | PRJNA612371 | Danio rerio strain:TU Raw sequence reads | PRJNA612371 | Whole Genome Sequencing | Hemorrhage stroke is a severe vascular disease of the brain with a high mortality rate in humans. Sodium tanshinone IIA sulfonate STS is a water soluble derivative of tanshinone IIA which is the main active ingredient of Salvia miltiorrhiza Bge known as Danshen in Chinese and has been approved as a commercial drug for treating cardiovascular disease by the China Food and Drug Administration. In our previous study we established a HMG COA inhibitor atorvastatin Ator induced zebrafish model of cerebral hemorrhage and found that STS dramatically decreased both the hemorrhage rate and hemorrhage area although the underlying mechanism was not fully elucidated. Therefore in the present study we conducted transcriptome analysis of the protective effect of STS against Ator induced cerebral hemorrhage in zebrafish using RNA Seq technology and further clarify its underlying molecular mechanisms on HIF 1 and its regulators i.e. the PI3K/Akt and MAPK signaling pathways were verified by real time PCR analysis and specific pharmacological inhibitors. We are also able to show that hemoglobin carbonic anhydrase Na+/H+ exchanger and HIF 1 genes might be potential biomarkers of Ator induced cerebral hemorrhage in zebrafish as well as pharmacological targets of STS. This study also provided evidence of bio markers involved in hemorrhage stroke and improved understanding of the effects of HMG COA inhibition on vascular permeability and cerebral hemorrhage. | Ator STS 2 zebrafish | strain:TU|isolate:not collected|breed:not collected|cultivar:not collected|ecotype:not collected|age:2dpf|dev stage:not collected|sex:not applicable|tissue:head|geo loc name:China:Shanghai|sample type:model organism|replicate:replicate=biological replicate 2'|BioSampleModel:Model organism or animal | Ator STS 2 zebrafish | Ator STS 2 zebrafish | Ator STS 2 zebrafish | transcriptome | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | BGISEQ | BGISEQ-500 | SRP279881 | Ator_STS_2_1.fq.gz | fastq | 1057950250.0 | 21159005.0 | Ator STS 2 1.fq.gz | 0:50 | A:286256354;C:238832868;G:244078781;T:288782247;N:0 | 50 | 286256354 | 238832868 | 244078781 | 288782247 | 0 | SRX9064849 | SRS7314022 | SRA1120721 | Shanghai University of Traditional Chinese Medicine|Longhua Hospital | Shanghai University of Traditional Chinese Medicine | 1 | 0.94307 | 0.10974 | 0.69631 | 0.4703 | 50 | B | usable mapping rate | bgi | bgi | unknown | poly_a | unknown | bulk | unknown | unknown | China | 2020-09-03 | Hatching | Embryo | Head | Nervous System | ||||||||||||||||||||||||||||
| 57276 | 57276 | SRR12577975 | SRX9064848 | SRS7314021 | SRP279881 | PRJNA612371 | Danio rerio strain:TU Raw sequence reads | PRJNA612371 | Whole Genome Sequencing | Hemorrhage stroke is a severe vascular disease of the brain with a high mortality rate in humans. Sodium tanshinone IIA sulfonate STS is a water soluble derivative of tanshinone IIA which is the main active ingredient of Salvia miltiorrhiza Bge known as Danshen in Chinese and has been approved as a commercial drug for treating cardiovascular disease by the China Food and Drug Administration. In our previous study we established a HMG COA inhibitor atorvastatin Ator induced zebrafish model of cerebral hemorrhage and found that STS dramatically decreased both the hemorrhage rate and hemorrhage area although the underlying mechanism was not fully elucidated. Therefore in the present study we conducted transcriptome analysis of the protective effect of STS against Ator induced cerebral hemorrhage in zebrafish using RNA Seq technology and further clarify its underlying molecular mechanisms on HIF 1 and its regulators i.e. the PI3K/Akt and MAPK signaling pathways were verified by real time PCR analysis and specific pharmacological inhibitors. We are also able to show that hemoglobin carbonic anhydrase Na+/H+ exchanger and HIF 1 genes might be potential biomarkers of Ator induced cerebral hemorrhage in zebrafish as well as pharmacological targets of STS. This study also provided evidence of bio markers involved in hemorrhage stroke and improved understanding of the effects of HMG COA inhibition on vascular permeability and cerebral hemorrhage. | Ator STS 1 zebrafish | strain:TU|isolate:not collected|breed:not collected|cultivar:not collected|ecotype:not collected|age:2dpf|dev stage:not collected|sex:not applicable|tissue:head|geo loc name:China:Shanghai|sample type:model organism|replicate:replicate=biological replicate 1'|BioSampleModel:Model organism or animal | Ator STS 1 zebrafish | Ator STS 1 zebrafish | Ator STS 1 zebrafish | transcriptome | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | BGISEQ | BGISEQ-500 | SRP279881 | Ator_STS_1_1.fq.gz | fastq | 1070136700.0 | 21402734.0 | Ator STS 1 1.fq.gz | 0:50 | A:297601042;C:243837062;G:243583907;T:285114689;N:0 | 50 | 297601042 | 243837062 | 243583907 | 285114689 | 0 | SRX9064848 | SRS7314021 | SRA1120721 | Shanghai University of Traditional Chinese Medicine|Longhua Hospital | Shanghai University of Traditional Chinese Medicine | 1 | 0.94665 | 0.12219 | 0.67953 | 0.46393 | 50 | B | usable mapping rate | bgi | bgi | unknown | poly_a | unknown | bulk | unknown | unknown | China | 2020-09-03 | Hatching | Embryo | Head | Nervous System | ||||||||||||||||||||||||||||
| 57277 | 57277 | SRR12577976 | SRX9064847 | SRS7314020 | SRP279881 | PRJNA612371 | Danio rerio strain:TU Raw sequence reads | PRJNA612371 | Whole Genome Sequencing | Hemorrhage stroke is a severe vascular disease of the brain with a high mortality rate in humans. Sodium tanshinone IIA sulfonate STS is a water soluble derivative of tanshinone IIA which is the main active ingredient of Salvia miltiorrhiza Bge known as Danshen in Chinese and has been approved as a commercial drug for treating cardiovascular disease by the China Food and Drug Administration. In our previous study we established a HMG COA inhibitor atorvastatin Ator induced zebrafish model of cerebral hemorrhage and found that STS dramatically decreased both the hemorrhage rate and hemorrhage area although the underlying mechanism was not fully elucidated. Therefore in the present study we conducted transcriptome analysis of the protective effect of STS against Ator induced cerebral hemorrhage in zebrafish using RNA Seq technology and further clarify its underlying molecular mechanisms on HIF 1 and its regulators i.e. the PI3K/Akt and MAPK signaling pathways were verified by real time PCR analysis and specific pharmacological inhibitors. We are also able to show that hemoglobin carbonic anhydrase Na+/H+ exchanger and HIF 1 genes might be potential biomarkers of Ator induced cerebral hemorrhage in zebrafish as well as pharmacological targets of STS. This study also provided evidence of bio markers involved in hemorrhage stroke and improved understanding of the effects of HMG COA inhibition on vascular permeability and cerebral hemorrhage. | Ator 3 zebrafish | strain:TU|isolate:not collected|breed:not collected|cultivar:not collected|ecotype:not collected|age:2dpf|dev stage:not collected|sex:not applicable|tissue:head|geo loc name:China:Shanghai|sample type:model organism|replicate:replicate=biological replicate 3 prime|BioSampleModel:Model organism or animal | Ator 3 zebrafish | Ator 3 zebrafish | Ator 3 zebrafish | transcriptome | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | BGISEQ | BGISEQ-500 | SRP279881 | Ator_3_1.fq.gz | fastq | 1058031900.0 | 21160638.0 | Ator 3 1.fq.gz | 0:50 | A:285399527;C:239993172;G:245571467;T:287067734;N:0 | 50 | 285399527 | 239993172 | 245571467 | 287067734 | 0 | SRX9064847 | SRS7314020 | SRA1120721 | Shanghai University of Traditional Chinese Medicine|Longhua Hospital | Shanghai University of Traditional Chinese Medicine | 1 | 0.94536 | 0.11052 | 0.6957 | 0.48195 | 50 | B | usable mapping rate | bgi | bgi | unknown | poly_a | unknown | bulk | unknown | unknown | China | 2020-09-03 | Hatching | Embryo | Head | Nervous System | ||||||||||||||||||||||||||||
| 57278 | 57278 | SRR12577977 | SRX9064846 | SRS7314019 | SRP279881 | PRJNA612371 | Danio rerio strain:TU Raw sequence reads | PRJNA612371 | Whole Genome Sequencing | Hemorrhage stroke is a severe vascular disease of the brain with a high mortality rate in humans. Sodium tanshinone IIA sulfonate STS is a water soluble derivative of tanshinone IIA which is the main active ingredient of Salvia miltiorrhiza Bge known as Danshen in Chinese and has been approved as a commercial drug for treating cardiovascular disease by the China Food and Drug Administration. In our previous study we established a HMG COA inhibitor atorvastatin Ator induced zebrafish model of cerebral hemorrhage and found that STS dramatically decreased both the hemorrhage rate and hemorrhage area although the underlying mechanism was not fully elucidated. Therefore in the present study we conducted transcriptome analysis of the protective effect of STS against Ator induced cerebral hemorrhage in zebrafish using RNA Seq technology and further clarify its underlying molecular mechanisms on HIF 1 and its regulators i.e. the PI3K/Akt and MAPK signaling pathways were verified by real time PCR analysis and specific pharmacological inhibitors. We are also able to show that hemoglobin carbonic anhydrase Na+/H+ exchanger and HIF 1 genes might be potential biomarkers of Ator induced cerebral hemorrhage in zebrafish as well as pharmacological targets of STS. This study also provided evidence of bio markers involved in hemorrhage stroke and improved understanding of the effects of HMG COA inhibition on vascular permeability and cerebral hemorrhage. | Ator 2 zebrafish | strain:TU|isolate:not collected|breed:not collected|cultivar:not collected|ecotype:not collected|age:2dpf|dev stage:not collected|sex:not applicable|tissue:head|geo loc name:China:Shanghai|sample type:model organism|replicate:replicate=biological replicate 2'|BioSampleModel:Model organism or animal | Ator 2 zebrafish | Ator 2 zebrafish | Ator 2 zebrafish | transcriptome | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | BGISEQ | BGISEQ-500 | SRP279881 | Ator_2_1.fq.gz | fastq | 1059618400.0 | 21192368.0 | Ator 2 1.fq.gz | 0:50 | A:283429136;C:243023053;G:248904818;T:284261393;N:0 | 50 | 283429136 | 243023053 | 248904818 | 284261393 | 0 | SRX9064846 | SRS7314019 | SRA1120721 | Shanghai University of Traditional Chinese Medicine|Longhua Hospital | Shanghai University of Traditional Chinese Medicine | 1 | 0.94743 | 0.09493 | 0.70218 | 0.475 | 50 | B | usable mapping rate | bgi | bgi | unknown | poly_a | unknown | bulk | unknown | unknown | China | 2020-09-03 | Hatching | Embryo | Head | Nervous System | ||||||||||||||||||||||||||||
| 57279 | 57279 | SRR12577978 | SRX9064845 | SRS7314018 | SRP279881 | PRJNA612371 | Danio rerio strain:TU Raw sequence reads | PRJNA612371 | Whole Genome Sequencing | Hemorrhage stroke is a severe vascular disease of the brain with a high mortality rate in humans. Sodium tanshinone IIA sulfonate STS is a water soluble derivative of tanshinone IIA which is the main active ingredient of Salvia miltiorrhiza Bge known as Danshen in Chinese and has been approved as a commercial drug for treating cardiovascular disease by the China Food and Drug Administration. In our previous study we established a HMG COA inhibitor atorvastatin Ator induced zebrafish model of cerebral hemorrhage and found that STS dramatically decreased both the hemorrhage rate and hemorrhage area although the underlying mechanism was not fully elucidated. Therefore in the present study we conducted transcriptome analysis of the protective effect of STS against Ator induced cerebral hemorrhage in zebrafish using RNA Seq technology and further clarify its underlying molecular mechanisms on HIF 1 and its regulators i.e. the PI3K/Akt and MAPK signaling pathways were verified by real time PCR analysis and specific pharmacological inhibitors. We are also able to show that hemoglobin carbonic anhydrase Na+/H+ exchanger and HIF 1 genes might be potential biomarkers of Ator induced cerebral hemorrhage in zebrafish as well as pharmacological targets of STS. This study also provided evidence of bio markers involved in hemorrhage stroke and improved understanding of the effects of HMG COA inhibition on vascular permeability and cerebral hemorrhage. | Ator 1 zebrafish | strain:TU|isolate:not collected|breed:not collected|cultivar:not collected|ecotype:not collected|age:2dpf|dev stage:not collected|sex:not applicable|tissue:head|geo loc name:China:Shanghai|sample type:model organism|replicate:replicate=biological replicate 1'|BioSampleModel:Model organism or animal | Ator 1 zebrafish | Ator 1 zebrafish | Ator 1 zebrafish | transcriptome | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | BGISEQ | BGISEQ-500 | SRP279881 | Ator_1_1.fq.gz | fastq | 1075695950.0 | 21513919.0 | Ator 1 1.fq.gz | 0:50 | A:297768036;C:244470267;G:243768925;T:289688722;N:0 | 50 | 297768036 | 244470267 | 243768925 | 289688722 | 0 | SRX9064845 | SRS7314018 | SRA1120721 | Shanghai University of Traditional Chinese Medicine|Longhua Hospital | Shanghai University of Traditional Chinese Medicine | 1 | 0.94468 | 0.13183 | 0.68363 | 0.46288 | 50 | B | usable mapping rate | bgi | bgi | unknown | poly_a | unknown | bulk | unknown | unknown | China | 2020-09-03 | Hatching | Embryo | Head | Nervous System |
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CREATE TABLE run_metadata("run.accession" VARCHAR, "experiment.accession" VARCHAR, "sample.accession" VARCHAR, "study.accession" VARCHAR, bioproject VARCHAR, "study.title" VARCHAR, "study.alias" VARCHAR, "study.type" VARCHAR, "study.abstract" VARCHAR, "study.attributes" VARCHAR, "study.PMIDs" VARCHAR, "sample.description" VARCHAR, "sample.title" VARCHAR, "sample.alias" VARCHAR, "sample.centername" VARCHAR, "sample.attributes" VARCHAR, "GEOsample.title" VARCHAR, "GEOsample.dataprocessing" VARCHAR, "GEOsample.source" VARCHAR, "GEOsample.treatmentprotocol" VARCHAR, "GEOsample.extractprotocol" VARCHAR, "GEOsample.growthprotocol" VARCHAR, "GEOsample.characteristics" VARCHAR, "GEOsample.accession" VARCHAR, "experiment.title" VARCHAR, "experiment.alias" VARCHAR, "experiment.library_name" VARCHAR, "experiment.design_description" VARCHAR, "experiment.library_construction_protocol" VARCHAR, "experiment.attributes" VARCHAR, "experiment.library_strategy" VARCHAR, "experiment.library_source" VARCHAR, "experiment.library_selection" VARCHAR, "experiment.library_layout" VARCHAR, "experiment.platform" VARCHAR, "experiment.instrument_model" VARCHAR, "experiment.spot_descriptor" VARCHAR, "experiment.study_ref" VARCHAR, "run.title" VARCHAR, "run.attributes" VARCHAR, "run.filename" VARCHAR, "run.semantic_name" VARCHAR, "run.total_bases" DOUBLE, "run.total_spots" DOUBLE, "run.alias" VARCHAR, "run.read_lengths" VARCHAR, "run.base_counts" VARCHAR, "run.r1_length" BIGINT, "run.r2_length" BIGINT, "run.r3_length" BIGINT, "run.r4_length" BIGINT, "run.Acount" BIGINT, "run.Ccount" BIGINT, "run.Gcount" BIGINT, "run.Tcount" BIGINT, "run.Ncount" BIGINT, "run.experiment" VARCHAR, "run.pool_member" VARCHAR, "submission.accession" VARCHAR, "submission.srasource" VARCHAR, "submission.bioprojectsource" VARCHAR, "seqdetective.n_mates" BIGINT, "seqdetective.mapping_rate.mate1" DOUBLE, "seqdetective.mapping_rate.mate2" DOUBLE, "seqdetective.nofeature_rate.mate1" DOUBLE, "seqdetective.nofeature_rate.mate2" DOUBLE, "seqdetective.sparsity.mate1" DOUBLE, "seqdetective.sparsity.mate2" DOUBLE, "seqdetective.pos_strand_rate.mate1" DOUBLE, "seqdetective.pos_strand_rate.mate2" DOUBLE, "seqdetective.readlen.mate1" BIGINT, "seqdetective.readlen.mate2" BIGINT, "seqdetective.judgement.mate1" VARCHAR, "seqdetective.judgement.mate2" VARCHAR, "seqdetective.judgement.reason" VARCHAR, platform_family VARCHAR, instrument_generation VARCHAR, read_bias VARCHAR, selection_class VARCHAR, prep_kit VARCHAR, sc_or_bulk VARCHAR, tech_class VARCHAR, technology VARCHAR, tech_variant VARCHAR, "submission.bioprojectsource.country" VARCHAR, earliest_date DATE, devstage_curation VARCHAR, devstage_curation_coarse VARCHAR, tissue_curation VARCHAR, tissue_curation_coarse VARCHAR);;
CREATE INDEX idx_run_bioproject ON run_metadata(bioproject);;
CREATE INDEX idx_run_run_accession ON run_metadata("run.accession");;