run_metadata
64 rows where experiment.library_layout = "SINGLE", experiment.library_selection = "CAGE" and technology = "unknown"
This data as json, CSV (advanced)
| Link | rowid ▼ | run.accession | experiment.accession | sample.accession | study.accession | bioproject | study.title | study.alias | study.type | study.abstract | study.attributes | study.PMIDs | sample.description | sample.title | sample.alias | sample.centername | sample.attributes | GEOsample.title | GEOsample.dataprocessing | GEOsample.source | GEOsample.treatmentprotocol | GEOsample.extractprotocol | GEOsample.growthprotocol | GEOsample.characteristics | GEOsample.accession | experiment.title | experiment.alias | experiment.library_name | experiment.design_description | experiment.library_construction_protocol | experiment.attributes | experiment.library_strategy | experiment.library_source | experiment.library_selection | experiment.library_layout | experiment.platform | experiment.instrument_model | experiment.spot_descriptor | experiment.study_ref | run.title | run.attributes | run.filename | run.semantic_name | run.total_bases | run.total_spots | run.alias | run.read_lengths | run.base_counts | run.r1_length | run.r2_length | run.r3_length | run.r4_length | run.Acount | run.Ccount | run.Gcount | run.Tcount | run.Ncount | run.experiment | run.pool_member | submission.accession | submission.srasource | submission.bioprojectsource | seqdetective.n_mates | seqdetective.mapping_rate.mate1 | seqdetective.mapping_rate.mate2 | seqdetective.nofeature_rate.mate1 | seqdetective.nofeature_rate.mate2 | seqdetective.sparsity.mate1 | seqdetective.sparsity.mate2 | seqdetective.pos_strand_rate.mate1 | seqdetective.pos_strand_rate.mate2 | seqdetective.readlen.mate1 | seqdetective.readlen.mate2 | seqdetective.judgement.mate1 | seqdetective.judgement.mate2 | seqdetective.judgement.reason | platform_family | instrument_generation | read_bias | selection_class | prep_kit | sc_or_bulk | tech_class | technology | tech_variant | submission.bioprojectsource.country | earliest_date | devstage_curation | devstage_curation_coarse | tissue_curation | tissue_curation_coarse |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 9803 | 9803 | ERR3909553 | ERX3918377 | ERS4309135 | ERP120006 | PRJEB36776 | CAGE seq of zebrafish cells extracted at the 14 somite stage and segregated by cell cycle dynamics e.g. fast green vs slow red | E-MTAB-8795 | Transcriptome Analysis | The core promoter a stretch of DNA surrounding the transcription start site TSS is a major integration point for regulatory signals controlling gene transcription. Cellular differentiation is marked by divergence in transcriptional repertoire and cell cycling behaviour between cells of different fates. The role promoter associated gene regulatory networks play in development associated transitions in cell cycle dynamics is poorly understood. This study demonstrates in a vertebrate embryo how core promoter variations define transcriptional output in cells transitioning from a proliferative to cell lineage specifying phenotype. Assessment of cell proliferation across zebrafish embryo development using the FUCCI transgenic cell cycle phase marker revealed a spatial and lineage specific separation in cell cycling behaviour. To investigate the role differential promoter usage plays in this process cap analysis of gene expression CAGE was performed on cells segregated by cycling dynamics. | ENA FIRST PUBLIC:2020 06 19|ENA LAST UPDATE:2020 02 13 | Protocols: Zebrafish were dechorionated and selected at the 14 somite stage 14 hpf The embryos were dissociated into a single cell suspension using enzyme free cell dissociation buffer PBS based Gibco. Dissociated cells were pelleted and resuspended in Hanks balanced salt solution without xxx chloride or magnesium sulphate Sigma for sorting. Cells were fluorescence associated cell sorted FACS into populations displaying red and green fluorescence. RNA was extracted from isolated cells using the miRNeasy kit Qiagen. RNA quality was analysed by capillary electrophoresis Bioanalyzer 2100 Agilent. All samples had an RNA integrity number RIN >9. NanoCAGE libraries were generated following a protocol described in: Poulain S. Kato S. Arnaud O. Morlighem J.E. Suzuki M. Plessy C. and Harbers M. 2017 NanoCAGE: A Method for the Analysis of Coding and Noncoding five prime Capped Transcriptomes. Methods Mol Biol 1543 57 109. | Total3 | SAMEA6544760 | UNIVERSITY OF BIRMINGHAM | ENA FIRST PUBLIC:2020 06 19T17:05:18Z|ENA LAST UPDATE:2020 02 13T15:36:56Z|External Id:SAMEA6544760|INSDC center name:UNIVERSITY OF BIRMINGHAM|INSDC first public:2020 06 19T17:05:18Z|INSDC last update:2020 02 13T15:36:56Z|INSDC status:public|Submitter Id:E MTAB 8795:Total3|age:14|broker name:ArrayExpress|common name:zebrafish|developmental stage:embryo|fraction:unsorted whole organism|organism part:whole organism|sample name:E MTAB 8795:Total3|scientific name:Danio rerio|sex:not available|strain:Dual FUCCI | Illumina HiSeq 2500 sequencing; CAGE seq of zebrafish cells extracted at the 14 somite stage and segregated by cell cycle dynamics e.g. fast green vs slow red | E MTAB 8795:Total3 s | Total3 s | CAGE seq of zebrafish cells extracted at the 14 somite stage and segregated by cell cycle dynamics e.g. fast green vs slow red | Zebrafish were dechorionated and selected at the 14 somite stage 14 hpf The embryos were dissociated into a single cell suspension using enzyme free cell dissociation buffer PBS based Gibco. Dissociated cells were pelleted and resuspended in Hanks balanced salt solution without xxx chloride or magnesium sulphate Sigma for sorting. Cells were fluorescence associated cell sorted FACS into populations displaying red and green fluorescence. RNA was extracted from isolated cells using the miRNeasy kit Qiagen. RNA quality was analysed by capillary electrophoresis Bioanalyzer 2100 Agilent. All samples had an RNA integrity number RIN >9. NanoCAGE libraries were generated following a protocol described in: Poulain S. Kato S. Arnaud O. Morlighem J.E. Suzuki M. Plessy C. and Harbers M. 2017 NanoCAGE: A Method for the Analysis of Coding and Noncoding five prime Capped Transcriptomes. Methods Mol Biol 1543 57 109. | Experimental Factor: fraction:unsorted whole organism | OTHER | TRANSCRIPTOMIC | CAGE | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP120006 | Illumina HiSeq 2500 sequencing; CAGE seq of zebrafish cells extracted at the 14 somite stage and segregated by cell cycle dynamics e.g. fast green vs slow red | ENA FIRST PUBLIC:2020 06 19|ENA LAST UPDATE:2020 02 25 | 14SomiteTotal3_TCGACG_L003_R1_001.fastq.gz | fastq | 652379310.0 | 14497318.0 | E MTAB 8795:Total3 | 0:45 1:0 | A:150840591;C:135992123;G:240098326;T:125390188;N:58082 | 45 | 0 | 150840591 | 135992123 | 240098326 | 125390188 | 58082 | ERX3918377 | ERS4309135 | ERA2381432 | UNIVERSITY OF BIRMINGHAM|European Nucleotide Archive | UNIVERSITY OF BIRMINGHAM|European Nucleotide Archive | 1 | 0.00539 | 0.00111 | 0.99582 | 0.74079 | 45 | T | under 1.2% mapping rate | illumina | hiseq_era | unknown | cage | unknown | bulk | unknown | unknown | United Kingdom | 2020-02-13 | Segmentation | Embryo | Whole Organism | All anatomical structures | |||||||||||||||||||
| 9804 | 9804 | ERR3909552 | ERX3918376 | ERS4309134 | ERP120006 | PRJEB36776 | CAGE seq of zebrafish cells extracted at the 14 somite stage and segregated by cell cycle dynamics e.g. fast green vs slow red | E-MTAB-8795 | Transcriptome Analysis | The core promoter a stretch of DNA surrounding the transcription start site TSS is a major integration point for regulatory signals controlling gene transcription. Cellular differentiation is marked by divergence in transcriptional repertoire and cell cycling behaviour between cells of different fates. The role promoter associated gene regulatory networks play in development associated transitions in cell cycle dynamics is poorly understood. This study demonstrates in a vertebrate embryo how core promoter variations define transcriptional output in cells transitioning from a proliferative to cell lineage specifying phenotype. Assessment of cell proliferation across zebrafish embryo development using the FUCCI transgenic cell cycle phase marker revealed a spatial and lineage specific separation in cell cycling behaviour. To investigate the role differential promoter usage plays in this process cap analysis of gene expression CAGE was performed on cells segregated by cycling dynamics. | ENA FIRST PUBLIC:2020 06 19|ENA LAST UPDATE:2020 02 13 | Protocols: Zebrafish were dechorionated and selected at the 14 somite stage 14 hpf The embryos were dissociated into a single cell suspension using enzyme free cell dissociation buffer PBS based Gibco. Dissociated cells were pelleted and resuspended in Hanks balanced salt solution without xxx chloride or magnesium sulphate Sigma for sorting. Cells were fluorescence associated cell sorted FACS into populations displaying red and green fluorescence. RNA was extracted from isolated cells using the miRNeasy kit Qiagen. RNA quality was analysed by capillary electrophoresis Bioanalyzer 2100 Agilent. All samples had an RNA integrity number RIN >9. NanoCAGE libraries were generated following a protocol described in: Poulain S. Kato S. Arnaud O. Morlighem J.E. Suzuki M. Plessy C. and Harbers M. 2017 NanoCAGE: A Method for the Analysis of Coding and Noncoding five prime Capped Transcriptomes. Methods Mol Biol 1543 57 109. | Total2 | SAMEA6544759 | UNIVERSITY OF BIRMINGHAM | ENA FIRST PUBLIC:2020 06 19T17:05:18Z|ENA LAST UPDATE:2020 02 13T15:36:56Z|External Id:SAMEA6544759|INSDC center name:UNIVERSITY OF BIRMINGHAM|INSDC first public:2020 06 19T17:05:18Z|INSDC last update:2020 02 13T15:36:56Z|INSDC status:public|Submitter Id:E MTAB 8795:Total2|age:14|broker name:ArrayExpress|common name:zebrafish|developmental stage:embryo|fraction:unsorted whole organism|organism part:whole organism|sample name:E MTAB 8795:Total2|scientific name:Danio rerio|sex:not available|strain:Dual FUCCI | Illumina HiSeq 2500 sequencing; CAGE seq of zebrafish cells extracted at the 14 somite stage and segregated by cell cycle dynamics e.g. fast green vs slow red | E MTAB 8795:Total2 s | Total2 s | CAGE seq of zebrafish cells extracted at the 14 somite stage and segregated by cell cycle dynamics e.g. fast green vs slow red | Zebrafish were dechorionated and selected at the 14 somite stage 14 hpf The embryos were dissociated into a single cell suspension using enzyme free cell dissociation buffer PBS based Gibco. Dissociated cells were pelleted and resuspended in Hanks balanced salt solution without xxx chloride or magnesium sulphate Sigma for sorting. Cells were fluorescence associated cell sorted FACS into populations displaying red and green fluorescence. RNA was extracted from isolated cells using the miRNeasy kit Qiagen. RNA quality was analysed by capillary electrophoresis Bioanalyzer 2100 Agilent. All samples had an RNA integrity number RIN >9. NanoCAGE libraries were generated following a protocol described in: Poulain S. Kato S. Arnaud O. Morlighem J.E. Suzuki M. Plessy C. and Harbers M. 2017 NanoCAGE: A Method for the Analysis of Coding and Noncoding five prime Capped Transcriptomes. Methods Mol Biol 1543 57 109. | Experimental Factor: fraction:unsorted whole organism | OTHER | TRANSCRIPTOMIC | CAGE | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP120006 | Illumina HiSeq 2500 sequencing; CAGE seq of zebrafish cells extracted at the 14 somite stage and segregated by cell cycle dynamics e.g. fast green vs slow red | ENA FIRST PUBLIC:2020 06 19|ENA LAST UPDATE:2020 02 25 | 14SomiteTotal2_TATAGC_L003_R1_001.fastq.gz | fastq | 457981740.0 | 10177372.0 | E MTAB 8795:Total2 | 0:45 1:0 | A:108846441;C:95327665;G:163111561;T:90654528;N:41545 | 45 | 0 | 108846441 | 95327665 | 163111561 | 90654528 | 41545 | ERX3918376 | ERS4309134 | ERA2381432 | UNIVERSITY OF BIRMINGHAM|European Nucleotide Archive | UNIVERSITY OF BIRMINGHAM|European Nucleotide Archive | 1 | 0.00612 | 0.00144 | 0.99387 | 0.66666 | 45 | T | under 1.2% mapping rate | illumina | hiseq_era | unknown | cage | unknown | bulk | unknown | unknown | United Kingdom | 2020-02-13 | Segmentation | Embryo | Whole Organism | All anatomical structures | |||||||||||||||||||
| 9805 | 9805 | ERR3909551 | ERX3918375 | ERS4309133 | ERP120006 | PRJEB36776 | CAGE seq of zebrafish cells extracted at the 14 somite stage and segregated by cell cycle dynamics e.g. fast green vs slow red | E-MTAB-8795 | Transcriptome Analysis | The core promoter a stretch of DNA surrounding the transcription start site TSS is a major integration point for regulatory signals controlling gene transcription. Cellular differentiation is marked by divergence in transcriptional repertoire and cell cycling behaviour between cells of different fates. The role promoter associated gene regulatory networks play in development associated transitions in cell cycle dynamics is poorly understood. This study demonstrates in a vertebrate embryo how core promoter variations define transcriptional output in cells transitioning from a proliferative to cell lineage specifying phenotype. Assessment of cell proliferation across zebrafish embryo development using the FUCCI transgenic cell cycle phase marker revealed a spatial and lineage specific separation in cell cycling behaviour. To investigate the role differential promoter usage plays in this process cap analysis of gene expression CAGE was performed on cells segregated by cycling dynamics. | ENA FIRST PUBLIC:2020 06 19|ENA LAST UPDATE:2020 02 13 | Protocols: Zebrafish were dechorionated and selected at the 14 somite stage 14 hpf The embryos were dissociated into a single cell suspension using enzyme free cell dissociation buffer PBS based Gibco. Dissociated cells were pelleted and resuspended in Hanks balanced salt solution without xxx chloride or magnesium sulphate Sigma for sorting. Cells were fluorescence associated cell sorted FACS into populations displaying red and green fluorescence. RNA was extracted from isolated cells using the miRNeasy kit Qiagen. RNA quality was analysed by capillary electrophoresis Bioanalyzer 2100 Agilent. All samples had an RNA integrity number RIN >9. NanoCAGE libraries were generated following a protocol described in: Poulain S. Kato S. Arnaud O. Morlighem J.E. Suzuki M. Plessy C. and Harbers M. 2017 NanoCAGE: A Method for the Analysis of Coding and Noncoding five prime Capped Transcriptomes. Methods Mol Biol 1543 57 109. | Total1 | SAMEA6544758 | UNIVERSITY OF BIRMINGHAM | ENA FIRST PUBLIC:2020 06 19T17:05:18Z|ENA LAST UPDATE:2020 02 13T15:36:56Z|External Id:SAMEA6544758|INSDC center name:UNIVERSITY OF BIRMINGHAM|INSDC first public:2020 06 19T17:05:18Z|INSDC last update:2020 02 13T15:36:56Z|INSDC status:public|Submitter Id:E MTAB 8795:Total1|age:14|broker name:ArrayExpress|common name:zebrafish|developmental stage:embryo|fraction:unsorted whole organism|organism part:whole organism|sample name:E MTAB 8795:Total1|scientific name:Danio rerio|sex:not available|strain:Dual FUCCI | Illumina HiSeq 2500 sequencing; CAGE seq of zebrafish cells extracted at the 14 somite stage and segregated by cell cycle dynamics e.g. fast green vs slow red | E MTAB 8795:Total1 s | Total1 s | CAGE seq of zebrafish cells extracted at the 14 somite stage and segregated by cell cycle dynamics e.g. fast green vs slow red | Zebrafish were dechorionated and selected at the 14 somite stage 14 hpf The embryos were dissociated into a single cell suspension using enzyme free cell dissociation buffer PBS based Gibco. Dissociated cells were pelleted and resuspended in Hanks balanced salt solution without xxx chloride or magnesium sulphate Sigma for sorting. Cells were fluorescence associated cell sorted FACS into populations displaying red and green fluorescence. RNA was extracted from isolated cells using the miRNeasy kit Qiagen. RNA quality was analysed by capillary electrophoresis Bioanalyzer 2100 Agilent. All samples had an RNA integrity number RIN >9. NanoCAGE libraries were generated following a protocol described in: Poulain S. Kato S. Arnaud O. Morlighem J.E. Suzuki M. Plessy C. and Harbers M. 2017 NanoCAGE: A Method for the Analysis of Coding and Noncoding five prime Capped Transcriptomes. Methods Mol Biol 1543 57 109. | Experimental Factor: fraction:unsorted whole organism | OTHER | TRANSCRIPTOMIC | CAGE | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP120006 | Illumina HiSeq 2500 sequencing; CAGE seq of zebrafish cells extracted at the 14 somite stage and segregated by cell cycle dynamics e.g. fast green vs slow red | ENA FIRST PUBLIC:2020 06 19|ENA LAST UPDATE:2020 02 25 | 14SomiteTotal1_GTATAC_L003_R1_001.fastq.gz | fastq | 778934655.0 | 17309659.0 | E MTAB 8795:Total1 | 0:45 1:0 | A:175694794;C:165046556;G:285795545;T:152324198;N:73562 | 45 | 0 | 175694794 | 165046556 | 285795545 | 152324198 | 73562 | ERX3918375 | ERS4309133 | ERA2381432 | UNIVERSITY OF BIRMINGHAM|European Nucleotide Archive | UNIVERSITY OF BIRMINGHAM|European Nucleotide Archive | 1 | 0.0048 | 0.00098 | 0.99492 | 0.69892 | 45 | T | under 1.2% mapping rate | illumina | hiseq_era | unknown | cage | unknown | bulk | unknown | unknown | United Kingdom | 2020-02-13 | Segmentation | Embryo | Whole Organism | All anatomical structures | |||||||||||||||||||
| 9806 | 9806 | ERR3909550 | ERX3918374 | ERS4309132 | ERP120006 | PRJEB36776 | CAGE seq of zebrafish cells extracted at the 14 somite stage and segregated by cell cycle dynamics e.g. fast green vs slow red | E-MTAB-8795 | Transcriptome Analysis | The core promoter a stretch of DNA surrounding the transcription start site TSS is a major integration point for regulatory signals controlling gene transcription. Cellular differentiation is marked by divergence in transcriptional repertoire and cell cycling behaviour between cells of different fates. The role promoter associated gene regulatory networks play in development associated transitions in cell cycle dynamics is poorly understood. This study demonstrates in a vertebrate embryo how core promoter variations define transcriptional output in cells transitioning from a proliferative to cell lineage specifying phenotype. Assessment of cell proliferation across zebrafish embryo development using the FUCCI transgenic cell cycle phase marker revealed a spatial and lineage specific separation in cell cycling behaviour. To investigate the role differential promoter usage plays in this process cap analysis of gene expression CAGE was performed on cells segregated by cycling dynamics. | ENA FIRST PUBLIC:2020 06 19|ENA LAST UPDATE:2020 02 13 | Protocols: Zebrafish were dechorionated and selected at the 14 somite stage 14 hpf The embryos were dissociated into a single cell suspension using enzyme free cell dissociation buffer PBS based Gibco. Dissociated cells were pelleted and resuspended in Hanks balanced salt solution without xxx chloride or magnesium sulphate Sigma for sorting. Cells were fluorescence associated cell sorted FACS into populations displaying red and green fluorescence. RNA was extracted from isolated cells using the miRNeasy kit Qiagen. RNA quality was analysed by capillary electrophoresis Bioanalyzer 2100 Agilent. All samples had an RNA integrity number RIN >9. NanoCAGE libraries were generated following a protocol described in: Poulain S. Kato S. Arnaud O. Morlighem J.E. Suzuki M. Plessy C. and Harbers M. 2017 NanoCAGE: A Method for the Analysis of Coding and Noncoding five prime Capped Transcriptomes. Methods Mol Biol 1543 57 109. | Red3 | SAMEA6544757 | UNIVERSITY OF BIRMINGHAM | ENA FIRST PUBLIC:2020 06 19T17:05:18Z|ENA LAST UPDATE:2020 02 13T15:36:56Z|External Id:SAMEA6544757|INSDC center name:UNIVERSITY OF BIRMINGHAM|INSDC first public:2020 06 19T17:05:18Z|INSDC last update:2020 02 13T15:36:56Z|INSDC status:public|Submitter Id:E MTAB 8795:Red3|age:14|broker name:ArrayExpress|common name:zebrafish|developmental stage:embryo|fraction:G1 slow cycling|organism part:whole organism|sample name:E MTAB 8795:Red3|scientific name:Danio rerio|sex:not available|strain:Dual FUCCI | Illumina HiSeq 2500 sequencing; CAGE seq of zebrafish cells extracted at the 14 somite stage and segregated by cell cycle dynamics e.g. fast green vs slow red | E MTAB 8795:Red3 s | Red3 s | CAGE seq of zebrafish cells extracted at the 14 somite stage and segregated by cell cycle dynamics e.g. fast green vs slow red | Zebrafish were dechorionated and selected at the 14 somite stage 14 hpf The embryos were dissociated into a single cell suspension using enzyme free cell dissociation buffer PBS based Gibco. Dissociated cells were pelleted and resuspended in Hanks balanced salt solution without xxx chloride or magnesium sulphate Sigma for sorting. Cells were fluorescence associated cell sorted FACS into populations displaying red and green fluorescence. RNA was extracted from isolated cells using the miRNeasy kit Qiagen. RNA quality was analysed by capillary electrophoresis Bioanalyzer 2100 Agilent. All samples had an RNA integrity number RIN >9. NanoCAGE libraries were generated following a protocol described in: Poulain S. Kato S. Arnaud O. Morlighem J.E. Suzuki M. Plessy C. and Harbers M. 2017 NanoCAGE: A Method for the Analysis of Coding and Noncoding five prime Capped Transcriptomes. Methods Mol Biol 1543 57 109. | Experimental Factor: fraction:G1 slow cycling | OTHER | TRANSCRIPTOMIC | CAGE | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP120006 | Illumina HiSeq 2500 sequencing; CAGE seq of zebrafish cells extracted at the 14 somite stage and segregated by cell cycle dynamics e.g. fast green vs slow red | ENA FIRST PUBLIC:2020 06 19|ENA LAST UPDATE:2020 02 25 | 14SomiteRed3_CACGAT_L003_R1_001.fastq.gz | fastq | 980271990.0 | 21783822.0 | E MTAB 8795:Red3 | 0:45 1:0 | A:231772782;C:201265416;G:341867307;T:205267905;N:98580 | 45 | 0 | 231772782 | 201265416 | 341867307 | 205267905 | 98580 | ERX3918374 | ERS4309132 | ERA2381432 | UNIVERSITY OF BIRMINGHAM|European Nucleotide Archive | UNIVERSITY OF BIRMINGHAM|European Nucleotide Archive | 1 | 0.0047 | 0.00138 | 0.99366 | 0.64343 | 45 | T | under 1.2% mapping rate | illumina | hiseq_era | unknown | cage | unknown | bulk | unknown | unknown | United Kingdom | 2020-02-13 | Segmentation | Embryo | Whole Organism | All anatomical structures | |||||||||||||||||||
| 9807 | 9807 | ERR3909549 | ERX3918373 | ERS4309131 | ERP120006 | PRJEB36776 | CAGE seq of zebrafish cells extracted at the 14 somite stage and segregated by cell cycle dynamics e.g. fast green vs slow red | E-MTAB-8795 | Transcriptome Analysis | The core promoter a stretch of DNA surrounding the transcription start site TSS is a major integration point for regulatory signals controlling gene transcription. Cellular differentiation is marked by divergence in transcriptional repertoire and cell cycling behaviour between cells of different fates. The role promoter associated gene regulatory networks play in development associated transitions in cell cycle dynamics is poorly understood. This study demonstrates in a vertebrate embryo how core promoter variations define transcriptional output in cells transitioning from a proliferative to cell lineage specifying phenotype. Assessment of cell proliferation across zebrafish embryo development using the FUCCI transgenic cell cycle phase marker revealed a spatial and lineage specific separation in cell cycling behaviour. To investigate the role differential promoter usage plays in this process cap analysis of gene expression CAGE was performed on cells segregated by cycling dynamics. | ENA FIRST PUBLIC:2020 06 19|ENA LAST UPDATE:2020 02 13 | Protocols: Zebrafish were dechorionated and selected at the 14 somite stage 14 hpf The embryos were dissociated into a single cell suspension using enzyme free cell dissociation buffer PBS based Gibco. Dissociated cells were pelleted and resuspended in Hanks balanced salt solution without xxx chloride or magnesium sulphate Sigma for sorting. Cells were fluorescence associated cell sorted FACS into populations displaying red and green fluorescence. RNA was extracted from isolated cells using the miRNeasy kit Qiagen. RNA quality was analysed by capillary electrophoresis Bioanalyzer 2100 Agilent. All samples had an RNA integrity number RIN >9. NanoCAGE libraries were generated following a protocol described in: Poulain S. Kato S. Arnaud O. Morlighem J.E. Suzuki M. Plessy C. and Harbers M. 2017 NanoCAGE: A Method for the Analysis of Coding and Noncoding five prime Capped Transcriptomes. Methods Mol Biol 1543 57 109. | Red2 | SAMEA6544756 | UNIVERSITY OF BIRMINGHAM | ENA FIRST PUBLIC:2020 06 19T17:05:18Z|ENA LAST UPDATE:2020 02 13T15:36:56Z|External Id:SAMEA6544756|INSDC center name:UNIVERSITY OF BIRMINGHAM|INSDC first public:2020 06 19T17:05:18Z|INSDC last update:2020 02 13T15:36:56Z|INSDC status:public|Submitter Id:E MTAB 8795:Red2|age:14|broker name:ArrayExpress|common name:zebrafish|developmental stage:embryo|fraction:G1 slow cycling|organism part:whole organism|sample name:E MTAB 8795:Red2|scientific name:Danio rerio|sex:not available|strain:Dual FUCCI | Illumina HiSeq 2500 sequencing; CAGE seq of zebrafish cells extracted at the 14 somite stage and segregated by cell cycle dynamics e.g. fast green vs slow red | E MTAB 8795:Red2 s | Red2 s | CAGE seq of zebrafish cells extracted at the 14 somite stage and segregated by cell cycle dynamics e.g. fast green vs slow red | Zebrafish were dechorionated and selected at the 14 somite stage 14 hpf The embryos were dissociated into a single cell suspension using enzyme free cell dissociation buffer PBS based Gibco. Dissociated cells were pelleted and resuspended in Hanks balanced salt solution without xxx chloride or magnesium sulphate Sigma for sorting. Cells were fluorescence associated cell sorted FACS into populations displaying red and green fluorescence. RNA was extracted from isolated cells using the miRNeasy kit Qiagen. RNA quality was analysed by capillary electrophoresis Bioanalyzer 2100 Agilent. All samples had an RNA integrity number RIN >9. NanoCAGE libraries were generated following a protocol described in: Poulain S. Kato S. Arnaud O. Morlighem J.E. Suzuki M. Plessy C. and Harbers M. 2017 NanoCAGE: A Method for the Analysis of Coding and Noncoding five prime Capped Transcriptomes. Methods Mol Biol 1543 57 109. | Experimental Factor: fraction:G1 slow cycling | OTHER | TRANSCRIPTOMIC | CAGE | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP120006 | Illumina HiSeq 2500 sequencing; CAGE seq of zebrafish cells extracted at the 14 somite stage and segregated by cell cycle dynamics e.g. fast green vs slow red | ENA FIRST PUBLIC:2020 06 19|ENA LAST UPDATE:2020 02 25 | 14SomiteRed2_ATCGTG_L003_R1_001.fastq.gz | fastq | 755095185.0 | 16779893.0 | E MTAB 8795:Red2 | 0:45 1:0 | A:173488674;C:161696782;G:267896203;T:151943387;N:70139 | 45 | 0 | 173488674 | 161696782 | 267896203 | 151943387 | 70139 | ERX3918373 | ERS4309131 | ERA2381432 | UNIVERSITY OF BIRMINGHAM|European Nucleotide Archive | UNIVERSITY OF BIRMINGHAM|European Nucleotide Archive | 1 | 0.00548 | 0.00113 | 0.99415 | 0.65826 | 45 | T | under 1.2% mapping rate | illumina | hiseq_era | unknown | cage | unknown | bulk | unknown | unknown | United Kingdom | 2020-02-13 | Segmentation | Embryo | Whole Organism | All anatomical structures | |||||||||||||||||||
| 9808 | 9808 | ERR3909548 | ERX3918372 | ERS4309130 | ERP120006 | PRJEB36776 | CAGE seq of zebrafish cells extracted at the 14 somite stage and segregated by cell cycle dynamics e.g. fast green vs slow red | E-MTAB-8795 | Transcriptome Analysis | The core promoter a stretch of DNA surrounding the transcription start site TSS is a major integration point for regulatory signals controlling gene transcription. Cellular differentiation is marked by divergence in transcriptional repertoire and cell cycling behaviour between cells of different fates. The role promoter associated gene regulatory networks play in development associated transitions in cell cycle dynamics is poorly understood. This study demonstrates in a vertebrate embryo how core promoter variations define transcriptional output in cells transitioning from a proliferative to cell lineage specifying phenotype. Assessment of cell proliferation across zebrafish embryo development using the FUCCI transgenic cell cycle phase marker revealed a spatial and lineage specific separation in cell cycling behaviour. To investigate the role differential promoter usage plays in this process cap analysis of gene expression CAGE was performed on cells segregated by cycling dynamics. | ENA FIRST PUBLIC:2020 06 19|ENA LAST UPDATE:2020 02 13 | Protocols: Zebrafish were dechorionated and selected at the 14 somite stage 14 hpf The embryos were dissociated into a single cell suspension using enzyme free cell dissociation buffer PBS based Gibco. Dissociated cells were pelleted and resuspended in Hanks balanced salt solution without xxx chloride or magnesium sulphate Sigma for sorting. Cells were fluorescence associated cell sorted FACS into populations displaying red and green fluorescence. RNA was extracted from isolated cells using the miRNeasy kit Qiagen. RNA quality was analysed by capillary electrophoresis Bioanalyzer 2100 Agilent. All samples had an RNA integrity number RIN >9. NanoCAGE libraries were generated following a protocol described in: Poulain S. Kato S. Arnaud O. Morlighem J.E. Suzuki M. Plessy C. and Harbers M. 2017 NanoCAGE: A Method for the Analysis of Coding and Noncoding five prime Capped Transcriptomes. Methods Mol Biol 1543 57 109. | Red1 | SAMEA6544755 | UNIVERSITY OF BIRMINGHAM | ENA FIRST PUBLIC:2020 06 19T17:05:18Z|ENA LAST UPDATE:2020 02 13T15:36:56Z|External Id:SAMEA6544755|INSDC center name:UNIVERSITY OF BIRMINGHAM|INSDC first public:2020 06 19T17:05:18Z|INSDC last update:2020 02 13T15:36:56Z|INSDC status:public|Submitter Id:E MTAB 8795:Red1|age:14|broker name:ArrayExpress|common name:zebrafish|developmental stage:embryo|fraction:G1 slow cycling|organism part:whole organism|sample name:E MTAB 8795:Red1|scientific name:Danio rerio|sex:not available|strain:Dual FUCCI | Illumina HiSeq 2500 sequencing; CAGE seq of zebrafish cells extracted at the 14 somite stage and segregated by cell cycle dynamics e.g. fast green vs slow red | E MTAB 8795:Red1 s | Red1 s | CAGE seq of zebrafish cells extracted at the 14 somite stage and segregated by cell cycle dynamics e.g. fast green vs slow red | Zebrafish were dechorionated and selected at the 14 somite stage 14 hpf The embryos were dissociated into a single cell suspension using enzyme free cell dissociation buffer PBS based Gibco. Dissociated cells were pelleted and resuspended in Hanks balanced salt solution without xxx chloride or magnesium sulphate Sigma for sorting. Cells were fluorescence associated cell sorted FACS into populations displaying red and green fluorescence. RNA was extracted from isolated cells using the miRNeasy kit Qiagen. RNA quality was analysed by capillary electrophoresis Bioanalyzer 2100 Agilent. All samples had an RNA integrity number RIN >9. NanoCAGE libraries were generated following a protocol described in: Poulain S. Kato S. Arnaud O. Morlighem J.E. Suzuki M. Plessy C. and Harbers M. 2017 NanoCAGE: A Method for the Analysis of Coding and Noncoding five prime Capped Transcriptomes. Methods Mol Biol 1543 57 109. | Experimental Factor: fraction:G1 slow cycling | OTHER | TRANSCRIPTOMIC | CAGE | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP120006 | Illumina HiSeq 2500 sequencing; CAGE seq of zebrafish cells extracted at the 14 somite stage and segregated by cell cycle dynamics e.g. fast green vs slow red | ENA FIRST PUBLIC:2020 06 19|ENA LAST UPDATE:2020 02 25 | 14SomiteRed1_ACAGAT_L003_R1_001.fastq.gz | fastq | 755180370.0 | 16781786.0 | E MTAB 8795:Red1 | 0:45 1:0 | A:179369537;C:157573419;G:260889303;T:157277751;N:70360 | 45 | 0 | 179369537 | 157573419 | 260889303 | 157277751 | 70360 | ERX3918372 | ERS4309130 | ERA2381432 | UNIVERSITY OF BIRMINGHAM|European Nucleotide Archive | UNIVERSITY OF BIRMINGHAM|European Nucleotide Archive | 1 | 0.00451 | 0.00099 | 0.99314 | 0.66248 | 45 | T | under 1.2% mapping rate | illumina | hiseq_era | unknown | cage | unknown | bulk | unknown | unknown | United Kingdom | 2020-02-13 | Segmentation | Embryo | Whole Organism | All anatomical structures | |||||||||||||||||||
| 9809 | 9809 | ERR3909547 | ERX3918371 | ERS4309129 | ERP120006 | PRJEB36776 | CAGE seq of zebrafish cells extracted at the 14 somite stage and segregated by cell cycle dynamics e.g. fast green vs slow red | E-MTAB-8795 | Transcriptome Analysis | The core promoter a stretch of DNA surrounding the transcription start site TSS is a major integration point for regulatory signals controlling gene transcription. Cellular differentiation is marked by divergence in transcriptional repertoire and cell cycling behaviour between cells of different fates. The role promoter associated gene regulatory networks play in development associated transitions in cell cycle dynamics is poorly understood. This study demonstrates in a vertebrate embryo how core promoter variations define transcriptional output in cells transitioning from a proliferative to cell lineage specifying phenotype. Assessment of cell proliferation across zebrafish embryo development using the FUCCI transgenic cell cycle phase marker revealed a spatial and lineage specific separation in cell cycling behaviour. To investigate the role differential promoter usage plays in this process cap analysis of gene expression CAGE was performed on cells segregated by cycling dynamics. | ENA FIRST PUBLIC:2020 06 19|ENA LAST UPDATE:2020 02 13 | Protocols: Zebrafish were dechorionated and selected at the 14 somite stage 14 hpf The embryos were dissociated into a single cell suspension using enzyme free cell dissociation buffer PBS based Gibco. Dissociated cells were pelleted and resuspended in Hanks balanced salt solution without xxx chloride or magnesium sulphate Sigma for sorting. Cells were fluorescence associated cell sorted FACS into populations displaying red and green fluorescence. RNA was extracted from isolated cells using the miRNeasy kit Qiagen. RNA quality was analysed by capillary electrophoresis Bioanalyzer 2100 Agilent. All samples had an RNA integrity number RIN >9. NanoCAGE libraries were generated following a protocol described in: Poulain S. Kato S. Arnaud O. Morlighem J.E. Suzuki M. Plessy C. and Harbers M. 2017 NanoCAGE: A Method for the Analysis of Coding and Noncoding five prime Capped Transcriptomes. Methods Mol Biol 1543 57 109. | Green3 | SAMEA6544754 | UNIVERSITY OF BIRMINGHAM | ENA FIRST PUBLIC:2020 06 19T17:05:18Z|ENA LAST UPDATE:2020 02 13T15:36:56Z|External Id:SAMEA6544754|INSDC center name:UNIVERSITY OF BIRMINGHAM|INSDC first public:2020 06 19T17:05:18Z|INSDC last update:2020 02 13T15:36:56Z|INSDC status:public|Submitter Id:E MTAB 8795:Green3|age:14|broker name:ArrayExpress|common name:zebrafish|developmental stage:embryo|fraction:S/G2/M rapid cycling|organism part:whole organism|sample name:E MTAB 8795:Green3|scientific name:Danio rerio|sex:not available|strain:Dual FUCCI | Illumina HiSeq 2500 sequencing; CAGE seq of zebrafish cells extracted at the 14 somite stage and segregated by cell cycle dynamics e.g. fast green vs slow red | E MTAB 8795:Green3 s | Green3 s | CAGE seq of zebrafish cells extracted at the 14 somite stage and segregated by cell cycle dynamics e.g. fast green vs slow red | Zebrafish were dechorionated and selected at the 14 somite stage 14 hpf The embryos were dissociated into a single cell suspension using enzyme free cell dissociation buffer PBS based Gibco. Dissociated cells were pelleted and resuspended in Hanks balanced salt solution without xxx chloride or magnesium sulphate Sigma for sorting. Cells were fluorescence associated cell sorted FACS into populations displaying red and green fluorescence. RNA was extracted from isolated cells using the miRNeasy kit Qiagen. RNA quality was analysed by capillary electrophoresis Bioanalyzer 2100 Agilent. All samples had an RNA integrity number RIN >9. NanoCAGE libraries were generated following a protocol described in: Poulain S. Kato S. Arnaud O. Morlighem J.E. Suzuki M. Plessy C. and Harbers M. 2017 NanoCAGE: A Method for the Analysis of Coding and Noncoding five prime Capped Transcriptomes. Methods Mol Biol 1543 57 109. | Experimental Factor: fraction:S/G2/M rapid cycling | OTHER | TRANSCRIPTOMIC | CAGE | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP120006 | Illumina HiSeq 2500 sequencing; CAGE seq of zebrafish cells extracted at the 14 somite stage and segregated by cell cycle dynamics e.g. fast green vs slow red | ENA FIRST PUBLIC:2020 06 19|ENA LAST UPDATE:2020 02 25 | 14SomiteGreen3_GAGTGA_L003_R1_001.fastq.gz | fastq | 819288855.0 | 18206419.0 | E MTAB 8795:Green3 | 0:45 1:0 | A:189173618;C:170597494;G:286250625;T:173188944;N:78174 | 45 | 0 | 189173618 | 170597494 | 286250625 | 173188944 | 78174 | ERX3918371 | ERS4309129 | ERA2381432 | UNIVERSITY OF BIRMINGHAM|European Nucleotide Archive | UNIVERSITY OF BIRMINGHAM|European Nucleotide Archive | 1 | 0.00439 | 0.001 | 0.99377 | 0.68367 | 45 | T | under 1.2% mapping rate | illumina | hiseq_era | unknown | cage | unknown | bulk | unknown | unknown | United Kingdom | 2020-02-13 | Segmentation | Embryo | Whole Organism | All anatomical structures | |||||||||||||||||||
| 9810 | 9810 | ERR3909546 | ERX3918370 | ERS4309128 | ERP120006 | PRJEB36776 | CAGE seq of zebrafish cells extracted at the 14 somite stage and segregated by cell cycle dynamics e.g. fast green vs slow red | E-MTAB-8795 | Transcriptome Analysis | The core promoter a stretch of DNA surrounding the transcription start site TSS is a major integration point for regulatory signals controlling gene transcription. Cellular differentiation is marked by divergence in transcriptional repertoire and cell cycling behaviour between cells of different fates. The role promoter associated gene regulatory networks play in development associated transitions in cell cycle dynamics is poorly understood. This study demonstrates in a vertebrate embryo how core promoter variations define transcriptional output in cells transitioning from a proliferative to cell lineage specifying phenotype. Assessment of cell proliferation across zebrafish embryo development using the FUCCI transgenic cell cycle phase marker revealed a spatial and lineage specific separation in cell cycling behaviour. To investigate the role differential promoter usage plays in this process cap analysis of gene expression CAGE was performed on cells segregated by cycling dynamics. | ENA FIRST PUBLIC:2020 06 19|ENA LAST UPDATE:2020 02 13 | Protocols: Zebrafish were dechorionated and selected at the 14 somite stage 14 hpf The embryos were dissociated into a single cell suspension using enzyme free cell dissociation buffer PBS based Gibco. Dissociated cells were pelleted and resuspended in Hanks balanced salt solution without xxx chloride or magnesium sulphate Sigma for sorting. Cells were fluorescence associated cell sorted FACS into populations displaying red and green fluorescence. RNA was extracted from isolated cells using the miRNeasy kit Qiagen. RNA quality was analysed by capillary electrophoresis Bioanalyzer 2100 Agilent. All samples had an RNA integrity number RIN >9. NanoCAGE libraries were generated following a protocol described in: Poulain S. Kato S. Arnaud O. Morlighem J.E. Suzuki M. Plessy C. and Harbers M. 2017 NanoCAGE: A Method for the Analysis of Coding and Noncoding five prime Capped Transcriptomes. Methods Mol Biol 1543 57 109. | Green2 | SAMEA6544753 | UNIVERSITY OF BIRMINGHAM | ENA FIRST PUBLIC:2020 06 19T17:05:18Z|ENA LAST UPDATE:2020 02 13T15:36:56Z|External Id:SAMEA6544753|INSDC center name:UNIVERSITY OF BIRMINGHAM|INSDC first public:2020 06 19T17:05:18Z|INSDC last update:2020 02 13T15:36:56Z|INSDC status:public|Submitter Id:E MTAB 8795:Green2|age:14|broker name:ArrayExpress|common name:zebrafish|developmental stage:embryo|fraction:S/G2/M rapid cycling|organism part:whole organism|sample name:E MTAB 8795:Green2|scientific name:Danio rerio|sex:not available|strain:Dual FUCCI | Illumina HiSeq 2500 sequencing; CAGE seq of zebrafish cells extracted at the 14 somite stage and segregated by cell cycle dynamics e.g. fast green vs slow red | E MTAB 8795:Green2 s | Green2 s | CAGE seq of zebrafish cells extracted at the 14 somite stage and segregated by cell cycle dynamics e.g. fast green vs slow red | Zebrafish were dechorionated and selected at the 14 somite stage 14 hpf The embryos were dissociated into a single cell suspension using enzyme free cell dissociation buffer PBS based Gibco. Dissociated cells were pelleted and resuspended in Hanks balanced salt solution without xxx chloride or magnesium sulphate Sigma for sorting. Cells were fluorescence associated cell sorted FACS into populations displaying red and green fluorescence. RNA was extracted from isolated cells using the miRNeasy kit Qiagen. RNA quality was analysed by capillary electrophoresis Bioanalyzer 2100 Agilent. All samples had an RNA integrity number RIN >9. NanoCAGE libraries were generated following a protocol described in: Poulain S. Kato S. Arnaud O. Morlighem J.E. Suzuki M. Plessy C. and Harbers M. 2017 NanoCAGE: A Method for the Analysis of Coding and Noncoding five prime Capped Transcriptomes. Methods Mol Biol 1543 57 109. | Experimental Factor: fraction:S/G2/M rapid cycling | OTHER | TRANSCRIPTOMIC | CAGE | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP120006 | Illumina HiSeq 2500 sequencing; CAGE seq of zebrafish cells extracted at the 14 somite stage and segregated by cell cycle dynamics e.g. fast green vs slow red | ENA FIRST PUBLIC:2020 06 19|ENA LAST UPDATE:2020 02 25 | 14SomiteGreen2_CTGACG_L003_R1_001.fastq.gz | fastq | 697971375.0 | 15510475.0 | E MTAB 8795:Green2 | 0:45 1:0 | A:160028926;C:147075253;G:245773112;T:145025474;N:68610 | 45 | 0 | 160028926 | 147075253 | 245773112 | 145025474 | 68610 | ERX3918370 | ERS4309128 | ERA2381432 | UNIVERSITY OF BIRMINGHAM|European Nucleotide Archive | UNIVERSITY OF BIRMINGHAM|European Nucleotide Archive | 1 | 0.00489 | 0.00085 | 0.99586 | 0.80163 | 45 | T | under 1.2% mapping rate | illumina | hiseq_era | unknown | cage | unknown | bulk | unknown | unknown | United Kingdom | 2020-02-13 | Segmentation | Embryo | Whole Organism | All anatomical structures | |||||||||||||||||||
| 9811 | 9811 | ERR3909545 | ERX3918369 | ERS4309127 | ERP120006 | PRJEB36776 | CAGE seq of zebrafish cells extracted at the 14 somite stage and segregated by cell cycle dynamics e.g. fast green vs slow red | E-MTAB-8795 | Transcriptome Analysis | The core promoter a stretch of DNA surrounding the transcription start site TSS is a major integration point for regulatory signals controlling gene transcription. Cellular differentiation is marked by divergence in transcriptional repertoire and cell cycling behaviour between cells of different fates. The role promoter associated gene regulatory networks play in development associated transitions in cell cycle dynamics is poorly understood. This study demonstrates in a vertebrate embryo how core promoter variations define transcriptional output in cells transitioning from a proliferative to cell lineage specifying phenotype. Assessment of cell proliferation across zebrafish embryo development using the FUCCI transgenic cell cycle phase marker revealed a spatial and lineage specific separation in cell cycling behaviour. To investigate the role differential promoter usage plays in this process cap analysis of gene expression CAGE was performed on cells segregated by cycling dynamics. | ENA FIRST PUBLIC:2020 06 19|ENA LAST UPDATE:2020 02 13 | Protocols: Zebrafish were dechorionated and selected at the 14 somite stage 14 hpf The embryos were dissociated into a single cell suspension using enzyme free cell dissociation buffer PBS based Gibco. Dissociated cells were pelleted and resuspended in Hanks balanced salt solution without xxx chloride or magnesium sulphate Sigma for sorting. Cells were fluorescence associated cell sorted FACS into populations displaying red and green fluorescence. RNA was extracted from isolated cells using the miRNeasy kit Qiagen. RNA quality was analysed by capillary electrophoresis Bioanalyzer 2100 Agilent. All samples had an RNA integrity number RIN >9. NanoCAGE libraries were generated following a protocol described in: Poulain S. Kato S. Arnaud O. Morlighem J.E. Suzuki M. Plessy C. and Harbers M. 2017 NanoCAGE: A Method for the Analysis of Coding and Noncoding five prime Capped Transcriptomes. Methods Mol Biol 1543 57 109. | Green1 | SAMEA6544752 | UNIVERSITY OF BIRMINGHAM | ENA FIRST PUBLIC:2020 06 19T17:05:18Z|ENA LAST UPDATE:2020 02 13T15:36:56Z|External Id:SAMEA6544752|INSDC center name:UNIVERSITY OF BIRMINGHAM|INSDC first public:2020 06 19T17:05:18Z|INSDC last update:2020 02 13T15:36:56Z|INSDC status:public|Submitter Id:E MTAB 8795:Green1|age:14|broker name:ArrayExpress|common name:zebrafish|developmental stage:embryo|fraction:S/G2/M rapid cycling|organism part:whole organism|sample name:E MTAB 8795:Green1|scientific name:Danio rerio|sex:not available|strain:Dual FUCCI | Illumina HiSeq 2500 sequencing; CAGE seq of zebrafish cells extracted at the 14 somite stage and segregated by cell cycle dynamics e.g. fast green vs slow red | E MTAB 8795:Green1 s | Green1 s | CAGE seq of zebrafish cells extracted at the 14 somite stage and segregated by cell cycle dynamics e.g. fast green vs slow red | Zebrafish were dechorionated and selected at the 14 somite stage 14 hpf The embryos were dissociated into a single cell suspension using enzyme free cell dissociation buffer PBS based Gibco. Dissociated cells were pelleted and resuspended in Hanks balanced salt solution without xxx chloride or magnesium sulphate Sigma for sorting. Cells were fluorescence associated cell sorted FACS into populations displaying red and green fluorescence. RNA was extracted from isolated cells using the miRNeasy kit Qiagen. RNA quality was analysed by capillary electrophoresis Bioanalyzer 2100 Agilent. All samples had an RNA integrity number RIN >9. NanoCAGE libraries were generated following a protocol described in: Poulain S. Kato S. Arnaud O. Morlighem J.E. Suzuki M. Plessy C. and Harbers M. 2017 NanoCAGE: A Method for the Analysis of Coding and Noncoding five prime Capped Transcriptomes. Methods Mol Biol 1543 57 109. | Experimental Factor: fraction:S/G2/M rapid cycling | OTHER | TRANSCRIPTOMIC | CAGE | SINGLE | ILLUMINA | Illumina HiSeq 2500 | ERP120006 | Illumina HiSeq 2500 sequencing; CAGE seq of zebrafish cells extracted at the 14 somite stage and segregated by cell cycle dynamics e.g. fast green vs slow red | ENA FIRST PUBLIC:2020 06 19|ENA LAST UPDATE:2020 02 25 | 14SomiteGreen1_CACTGA_L003_R1_001.fastq.gz | fastq | 826595640.0 | 18368792.0 | E MTAB 8795:Green1 | 0:45 1:0 | A:193728088;C:171605661;G:287824557;T:173352680;N:84654 | 45 | 0 | 193728088 | 171605661 | 287824557 | 173352680 | 84654 | ERX3918369 | ERS4309127 | ERA2381432 | UNIVERSITY OF BIRMINGHAM|European Nucleotide Archive | UNIVERSITY OF BIRMINGHAM|European Nucleotide Archive | 1 | 0.00505 | 0.00101 | 0.99287 | 0.58198 | 45 | T | under 1.2% mapping rate | illumina | hiseq_era | unknown | cage | unknown | bulk | unknown | unknown | United Kingdom | 2020-02-13 | Segmentation | Embryo | Whole Organism | All anatomical structures | |||||||||||||||||||
| 36409 | 36409 | SRR516560 | SRX156356 | SRS347213 | SRP013950 | PRJNA169500 | Danio rerio embryonic promoterome | PRJNA169500 | Transcriptome Analysis | Goal of this study is to generate genome wide maps of transcription initiation throughout early embryonic development of zebrafish Danio rerio. Cap analysis of gene expression CAGE is used to detect transcription start sites at 1bp resolution. CAGE data is complemented by ChIPseq datasets for promoter associated histone modifications to study dynamic changes of promoter usage and chromatin configuration throughout early embryonic development. | pubmed:24531765 | Zebrafish wild type AB strain embryo prim20 stage | D. rerio prim20 embryo | D. rerio prim20 embryo | CAGE D. rerio prim20 embryo | CAGE D. rerio prim20 embryo run2 | D. rerio prim20 embryo | 1 | OTHER | TRANSCRIPTOMIC | CAGE | SINGLE | ILLUMINA | Illumina Genome Analyzer IIx | <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>27</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> | SRP013950 | CAGE_prim20_run1.fastq | fastq | 76770666.0 | 2843358.0 | CAGE D. rerio prim20 embryo run1 | 0:27 | A:19320047;C:17585086;G:22511175;T:17354358;N:0 | 27 | 19320047 | 17585086 | 22511175 | 17354358 | 0 | SRX156356 | SRS347213 | SRA055273 | University of Bergen | ZEPROME consortium | 1 | 0.56472 | 0.10044 | 0.77469 | 0.74192 | 27 | B | usable mapping rate | illumina | early_illumina | unknown | cage | unknown | bulk | unknown | unknown | Unknown | 2012-06-28 | Undetermined | Embryo | Embryo Imprecise | All anatomical structures | |||||||||||||||||||||||||
| 36410 | 36410 | SRR516561 | SRX156356 | SRS347213 | SRP013950 | PRJNA169500 | Danio rerio embryonic promoterome | PRJNA169500 | Transcriptome Analysis | Goal of this study is to generate genome wide maps of transcription initiation throughout early embryonic development of zebrafish Danio rerio. Cap analysis of gene expression CAGE is used to detect transcription start sites at 1bp resolution. CAGE data is complemented by ChIPseq datasets for promoter associated histone modifications to study dynamic changes of promoter usage and chromatin configuration throughout early embryonic development. | pubmed:24531765 | Zebrafish wild type AB strain embryo prim20 stage | D. rerio prim20 embryo | D. rerio prim20 embryo | CAGE D. rerio prim20 embryo | CAGE D. rerio prim20 embryo run2 | D. rerio prim20 embryo | 1 | OTHER | TRANSCRIPTOMIC | CAGE | SINGLE | ILLUMINA | Illumina Genome Analyzer IIx | <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>27</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> | SRP013950 | CAGE_prim20_run2.fastq | fastq | 163254906.0 | 6046478.0 | CAGE D. rerio prim20 embryo run2 | 0:27 | A:40211042;C:36959797;G:46866669;T:39217398;N:0 | 27 | 40211042 | 36959797 | 46866669 | 39217398 | 0 | SRX156356 | SRS347213 | SRA055273 | University of Bergen | ZEPROME consortium | 1 | 0.47475 | 0.06947 | 0.78409 | 0.78014 | 27 | B | usable mapping rate | illumina | early_illumina | unknown | cage | unknown | bulk | unknown | unknown | Unknown | 2013-08-31 | Undetermined | Embryo | Embryo Imprecise | All anatomical structures | |||||||||||||||||||||||||
| 36411 | 36411 | SRR516559 | SRX156355 | SRS347212 | SRP013950 | PRJNA169500 | Danio rerio embryonic promoterome | PRJNA169500 | Transcriptome Analysis | Goal of this study is to generate genome wide maps of transcription initiation throughout early embryonic development of zebrafish Danio rerio. Cap analysis of gene expression CAGE is used to detect transcription start sites at 1bp resolution. CAGE data is complemented by ChIPseq datasets for promoter associated histone modifications to study dynamic changes of promoter usage and chromatin configuration throughout early embryonic development. | pubmed:24531765 | Zebrafish wild type AB strain embryo prim6 stage | D. rerio prim6 embryo | D. rerio prim6 embryo | CAGE D. rerio prim6 embryo | CAGE D. rerio prim6 embryo | D. rerio prim6 embryo | 1 | OTHER | TRANSCRIPTOMIC | CAGE | SINGLE | ILLUMINA | Illumina Genome Analyzer IIx | <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>27</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> | SRP013950 | CAGE_prim6.fastq | fastq | 283414275.0 | 10496825.0 | CAGE D. rerio prim6 embryo | 0:27 | A:70974303;C:66202040;G:80064124;T:66173808;N:0 | 27 | 70974303 | 66202040 | 80064124 | 66173808 | 0 | SRX156355 | SRS347212 | SRA055273 | University of Bergen | ZEPROME consortium | 1 | 0.53395 | 0.07806 | 0.78255 | 0.77575 | 27 | B | usable mapping rate | illumina | early_illumina | unknown | cage | unknown | bulk | unknown | unknown | Unknown | 2013-08-31 | Undetermined | Embryo | Embryo Imprecise | All anatomical structures | |||||||||||||||||||||||||
| 36412 | 36412 | SRR516557 | SRX156352 | SRS347211 | SRP013950 | PRJNA169500 | Danio rerio embryonic promoterome | PRJNA169500 | Transcriptome Analysis | Goal of this study is to generate genome wide maps of transcription initiation throughout early embryonic development of zebrafish Danio rerio. Cap analysis of gene expression CAGE is used to detect transcription start sites at 1bp resolution. CAGE data is complemented by ChIPseq datasets for promoter associated histone modifications to study dynamic changes of promoter usage and chromatin configuration throughout early embryonic development. | pubmed:24531765 | Zebrafish wild type AB strain embryo 14 somites stage | D. rerio 14 somites embryo | D. rerio 14 somites embryo | CAGE D. rerio 14 somites embryo | CAGE D. rerio 14 somites embryo run2 | D. rerio 14 somites embryo | 1 | OTHER | TRANSCRIPTOMIC | CAGE | SINGLE | ILLUMINA | Illumina Genome Analyzer IIx | <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>27</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> | SRP013950 | 73689048.0 | 2729224.0 | CAGE D. rerio 14 somites embryo run1 | 0:27 | A:19506796;C:16238588;G:21636330;T:16307334;N:0 | 27 | 19506796 | 16238588 | 21636330 | 16307334 | 0 | SRX156352 | SRS347211 | SRA055273 | University of Bergen | ZEPROME consortium | 1 | 0.63348 | 0.09461 | 0.76199 | 0.68697 | 27 | B | usable mapping rate | illumina | early_illumina | unknown | cage | unknown | bulk | unknown | unknown | Unknown | 2012-06-28 | Segmentation | Embryo | Embryo Imprecise | All anatomical structures | |||||||||||||||||||||||||||
| 36413 | 36413 | SRR516558 | SRX156352 | SRS347211 | SRP013950 | PRJNA169500 | Danio rerio embryonic promoterome | PRJNA169500 | Transcriptome Analysis | Goal of this study is to generate genome wide maps of transcription initiation throughout early embryonic development of zebrafish Danio rerio. Cap analysis of gene expression CAGE is used to detect transcription start sites at 1bp resolution. CAGE data is complemented by ChIPseq datasets for promoter associated histone modifications to study dynamic changes of promoter usage and chromatin configuration throughout early embryonic development. | pubmed:24531765 | Zebrafish wild type AB strain embryo 14 somites stage | D. rerio 14 somites embryo | D. rerio 14 somites embryo | CAGE D. rerio 14 somites embryo | CAGE D. rerio 14 somites embryo run2 | D. rerio 14 somites embryo | 1 | OTHER | TRANSCRIPTOMIC | CAGE | SINGLE | ILLUMINA | Illumina Genome Analyzer IIx | <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>27</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> | SRP013950 | CAGE_14somites_run2.fastq | fastq | 212381811.0 | 7865993.0 | CAGE D. rerio 14 somites embryo run2 | 0:27 | A:53780820;C:47467464;G:59893810;T:51239717;N:0 | 27 | 53780820 | 47467464 | 59893810 | 51239717 | 0 | SRX156352 | SRS347211 | SRA055273 | University of Bergen | ZEPROME consortium | 1 | 0.5278 | 0.0806 | 0.77589 | 0.72881 | 27 | B | usable mapping rate | illumina | early_illumina | unknown | cage | unknown | bulk | unknown | unknown | Unknown | 2012-06-28 | Segmentation | Embryo | Embryo Imprecise | All anatomical structures | |||||||||||||||||||||||||
| 36414 | 36414 | SRR516555 | SRX156350 | SRS347210 | SRP013950 | PRJNA169500 | Danio rerio embryonic promoterome | PRJNA169500 | Transcriptome Analysis | Goal of this study is to generate genome wide maps of transcription initiation throughout early embryonic development of zebrafish Danio rerio. Cap analysis of gene expression CAGE is used to detect transcription start sites at 1bp resolution. CAGE data is complemented by ChIPseq datasets for promoter associated histone modifications to study dynamic changes of promoter usage and chromatin configuration throughout early embryonic development. | pubmed:24531765 | Zebrafish wild type AB strain embryo shield stage | D. rerio shield embryo | D. rerio shield embryo | CAGE D. rerio shield embryo | CAGE D. rerio shield embryo run2 | D. rerio shield embryo | 1 | OTHER | TRANSCRIPTOMIC | CAGE | SINGLE | ILLUMINA | Illumina Genome Analyzer IIx | <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>27</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> | SRP013950 | 81086886.0 | 3003218.0 | CAGE D. rerio shield embryo run1 | 0:27 | A:20800196;C:18582826;G:23491216;T:18212648;N:0 | 27 | 20800196 | 18582826 | 23491216 | 18212648 | 0 | SRX156350 | SRS347210 | SRA055273 | University of Bergen | ZEPROME consortium | 1 | 0.60434 | 0.09948 | 0.80432 | 0.72631 | 27 | B | usable mapping rate | illumina | early_illumina | unknown | cage | unknown | bulk | unknown | unknown | Unknown | 2012-06-28 | Gastrula | Embryo | Embryo Imprecise | All anatomical structures | |||||||||||||||||||||||||||
| 36415 | 36415 | SRR516556 | SRX156350 | SRS347210 | SRP013950 | PRJNA169500 | Danio rerio embryonic promoterome | PRJNA169500 | Transcriptome Analysis | Goal of this study is to generate genome wide maps of transcription initiation throughout early embryonic development of zebrafish Danio rerio. Cap analysis of gene expression CAGE is used to detect transcription start sites at 1bp resolution. CAGE data is complemented by ChIPseq datasets for promoter associated histone modifications to study dynamic changes of promoter usage and chromatin configuration throughout early embryonic development. | pubmed:24531765 | Zebrafish wild type AB strain embryo shield stage | D. rerio shield embryo | D. rerio shield embryo | CAGE D. rerio shield embryo | CAGE D. rerio shield embryo run2 | D. rerio shield embryo | 1 | OTHER | TRANSCRIPTOMIC | CAGE | SINGLE | ILLUMINA | Illumina Genome Analyzer IIx | <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>27</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> | SRP013950 | CAGE_shield_run2.fastq | fastq | 81675891.0 | 3025033.0 | CAGE D. rerio shield embryo run2 | 0:27 | A:21590166;C:17662459;G:22692127;T:19731139;N:0 | 27 | 21590166 | 17662459 | 22692127 | 19731139 | 0 | SRX156350 | SRS347210 | SRA055273 | University of Bergen | ZEPROME consortium | 1 | 0.52624 | 0.08062 | 0.81793 | 0.76291 | 27 | B | usable mapping rate | illumina | early_illumina | unknown | cage | unknown | bulk | unknown | unknown | Unknown | 2012-06-28 | Gastrula | Embryo | Embryo Imprecise | All anatomical structures | |||||||||||||||||||||||||
| 36416 | 36416 | SRR516554 | SRX156349 | SRS347209 | SRP013950 | PRJNA169500 | Danio rerio embryonic promoterome | PRJNA169500 | Transcriptome Analysis | Goal of this study is to generate genome wide maps of transcription initiation throughout early embryonic development of zebrafish Danio rerio. Cap analysis of gene expression CAGE is used to detect transcription start sites at 1bp resolution. CAGE data is complemented by ChIPseq datasets for promoter associated histone modifications to study dynamic changes of promoter usage and chromatin configuration throughout early embryonic development. | pubmed:24531765 | Zebrafish wild type AB strain embryo dome/zfs:0000015 stage | D. rerio dome/zfs:0000015 embryo | D. rerio dome/zfs:0000015 embryo | CAGE D. rerio dome/zfs:0000015 embryo | CAGE D. rerio dome/zfs:0000015 embryo | D. rerio dome/zfs:0000015 embryo | 1 | OTHER | TRANSCRIPTOMIC | CAGE | SINGLE | ILLUMINA | Illumina Genome Analyzer IIx | <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>27</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> | SRP013950 | CAGE_30p_dome.fastq | fastq | 165690819.0 | 6136697.0 | CAGE D. rerio dome/zfs:0000015 embryo | 0:27 | A:42721293;C:36492232;G:46154926;T:40322368;N:0 | 27 | 42721293 | 36492232 | 46154926 | 40322368 | 0 | SRX156349 | SRS347209 | SRA055273 | University of Bergen | ZEPROME consortium | 1 | 0.50236 | 0.0905 | 0.81824 | 0.8156 | 27 | B | usable mapping rate | illumina | early_illumina | unknown | cage | unknown | bulk | unknown | unknown | Unknown | 2012-06-28 | Blastula | Embryo | Embryo Imprecise | All anatomical structures | |||||||||||||||||||||||||
| 36417 | 36417 | SRR516552 | SRX156347 | SRS347208 | SRP013950 | PRJNA169500 | Danio rerio embryonic promoterome | PRJNA169500 | Transcriptome Analysis | Goal of this study is to generate genome wide maps of transcription initiation throughout early embryonic development of zebrafish Danio rerio. Cap analysis of gene expression CAGE is used to detect transcription start sites at 1bp resolution. CAGE data is complemented by ChIPseq datasets for promoter associated histone modifications to study dynamic changes of promoter usage and chromatin configuration throughout early embryonic development. | pubmed:24531765 | Zebrafish wild type AB strain embryo sphere/dome stage | D. rerio sphere/dome embryo | D. rerio sphere/dome embryo | CAGE D. rerio sphere/dome embryo | CAGE D. rerio sphere/dome embryo run2 | D. rerio sphere/dome embryo | 1 | OTHER | TRANSCRIPTOMIC | CAGE | SINGLE | ILLUMINA | Illumina Genome Analyzer IIx | <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>27</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> | SRP013950 | CAGE_sphere_dome_run1.fastq | fastq | 78937740.0 | 2923620.0 | CAGE D. rerio sphere/dome embryo run1 | 0:27 | A:20705496;C:17020957;G:22237043;T:18974244;N:0 | 27 | 20705496 | 17020957 | 22237043 | 18974244 | 0 | SRX156347 | SRS347208 | SRA055273 | University of Bergen | ZEPROME consortium | 1 | 0.50917 | 0.09193 | 0.81523 | 0.8084 | 27 | B | usable mapping rate | illumina | early_illumina | unknown | cage | unknown | bulk | unknown | unknown | Unknown | 2012-06-28 | Blastula | Embryo | Embryo Imprecise | All anatomical structures | |||||||||||||||||||||||||
| 36418 | 36418 | SRR516553 | SRX156347 | SRS347208 | SRP013950 | PRJNA169500 | Danio rerio embryonic promoterome | PRJNA169500 | Transcriptome Analysis | Goal of this study is to generate genome wide maps of transcription initiation throughout early embryonic development of zebrafish Danio rerio. Cap analysis of gene expression CAGE is used to detect transcription start sites at 1bp resolution. CAGE data is complemented by ChIPseq datasets for promoter associated histone modifications to study dynamic changes of promoter usage and chromatin configuration throughout early embryonic development. | pubmed:24531765 | Zebrafish wild type AB strain embryo sphere/dome stage | D. rerio sphere/dome embryo | D. rerio sphere/dome embryo | CAGE D. rerio sphere/dome embryo | CAGE D. rerio sphere/dome embryo run2 | D. rerio sphere/dome embryo | 1 | OTHER | TRANSCRIPTOMIC | CAGE | SINGLE | ILLUMINA | Illumina Genome Analyzer IIx | <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>27</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> | SRP013950 | CAGE_sphere_dome_run2.fastq | fastq | 82071009.0 | 3039667.0 | CAGE D. rerio sphere/dome embryo run2 | 0:27 | A:20823133;C:17914934;G:22973465;T:20359477;N:0 | 27 | 20823133 | 17914934 | 22973465 | 20359477 | 0 | SRX156347 | SRS347208 | SRA055273 | University of Bergen | ZEPROME consortium | 1 | 0.47213 | 0.07583 | 0.82384 | 0.8313 | 27 | B | usable mapping rate | illumina | early_illumina | unknown | cage | unknown | bulk | unknown | unknown | Unknown | 2012-06-28 | Blastula | Embryo | Embryo Imprecise | All anatomical structures | |||||||||||||||||||||||||
| 36419 | 36419 | SRR516551 | SRX156338 | SRS347207 | SRP013950 | PRJNA169500 | Danio rerio embryonic promoterome | PRJNA169500 | Transcriptome Analysis | Goal of this study is to generate genome wide maps of transcription initiation throughout early embryonic development of zebrafish Danio rerio. Cap analysis of gene expression CAGE is used to detect transcription start sites at 1bp resolution. CAGE data is complemented by ChIPseq datasets for promoter associated histone modifications to study dynamic changes of promoter usage and chromatin configuration throughout early embryonic development. | pubmed:24531765 | Zebrafish wild type AB strain embryo oblong stage | D. rerio oblong embryo | D. rerio oblong embryo | CAGE D. rerio oblong embryo | CAGE D. rerio oblong embryo | D. rerio oblong embryo | 1 | OTHER | TRANSCRIPTOMIC | CAGE | SINGLE | ILLUMINA | Illumina Genome Analyzer IIx | <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>27</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> | SRP013950 | 135892080.0 | 5033040.0 | CAGE D. rerio oblong embryo | 0:27 | A:34565898;C:30442218;G:38967845;T:31916119;N:0 | 27 | 34565898 | 30442218 | 38967845 | 31916119 | 0 | SRX156338 | SRS347207 | SRA055273 | University of Bergen | ZEPROME consortium | 1 | 0.56265 | 0.09335 | 0.80068 | 0.70759 | 27 | B | usable mapping rate | illumina | early_illumina | unknown | cage | unknown | bulk | unknown | unknown | Unknown | 2012-06-28 | Blastula | Embryo | Embryo Imprecise | All anatomical structures | |||||||||||||||||||||||||||
| 36420 | 36420 | SRR516550 | SRX156337 | SRS347206 | SRP013950 | PRJNA169500 | Danio rerio embryonic promoterome | PRJNA169500 | Transcriptome Analysis | Goal of this study is to generate genome wide maps of transcription initiation throughout early embryonic development of zebrafish Danio rerio. Cap analysis of gene expression CAGE is used to detect transcription start sites at 1bp resolution. CAGE data is complemented by ChIPseq datasets for promoter associated histone modifications to study dynamic changes of promoter usage and chromatin configuration throughout early embryonic development. | pubmed:24531765 | Zebrafish wild type AB strain embryo high stage | D. rerio high embryo | D. rerio high embryo | CAGE D. rerio high embryo | CAGE D. rerio high embryo | D. rerio high embryo | 1 | OTHER | TRANSCRIPTOMIC | CAGE | SINGLE | ILLUMINA | Illumina Genome Analyzer IIx | <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>27</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> | SRP013950 | CAGE_high.fastq | fastq | 128592846.0 | 4762698.0 | CAGE D. rerio high embryo | 0:27 | A:33693838;C:27629256;G:36692263;T:30577489;N:0 | 27 | 33693838 | 27629256 | 36692263 | 30577489 | 0 | SRX156337 | SRS347206 | SRA055273 | University of Bergen | ZEPROME consortium | 1 | 0.53282 | 0.08323 | 0.80854 | 0.77395 | 27 | B | usable mapping rate | illumina | early_illumina | unknown | cage | unknown | bulk | unknown | unknown | Unknown | 2013-08-31 | Undetermined | Embryo | Embryo Imprecise | All anatomical structures | |||||||||||||||||||||||||
| 36421 | 36421 | SRR516549 | SRX156336 | SRS347204 | SRP013950 | PRJNA169500 | Danio rerio embryonic promoterome | PRJNA169500 | Transcriptome Analysis | Goal of this study is to generate genome wide maps of transcription initiation throughout early embryonic development of zebrafish Danio rerio. Cap analysis of gene expression CAGE is used to detect transcription start sites at 1bp resolution. CAGE data is complemented by ChIPseq datasets for promoter associated histone modifications to study dynamic changes of promoter usage and chromatin configuration throughout early embryonic development. | pubmed:24531765 | Zebrafish wild type AB strain embryo 512 cells stage | D. rerio 512 cells embryo | D. rerio 512 cells embyo | CAGE D. rerio 512 cells embryo | CAGE D. rerio 512 cells embryo | D. rerio 512 cells embryo | 1 | OTHER | TRANSCRIPTOMIC | CAGE | SINGLE | ILLUMINA | Illumina Genome Analyzer IIx | <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>27</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> | SRP013950 | CAGE_512cells.fastq | fastq | 150228810.0 | 5564030.0 | CAGE D. rerio 512 cells embryo | 0:27 | A:37826960;C:33838922;G:43403014;T:35159914;N:0 | 27 | 37826960 | 33838922 | 43403014 | 35159914 | 0 | SRX156336 | SRS347204 | SRA055273 | University of Bergen | ZEPROME consortium | 1 | 0.57925 | 0.08753 | 0.79933 | 0.72579 | 27 | B | usable mapping rate | illumina | early_illumina | unknown | cage | unknown | bulk | unknown | unknown | Unknown | 2012-06-28 | Blastula | Embryo | Embryo Imprecise | All anatomical structures | |||||||||||||||||||||||||
| 36422 | 36422 | SRR516548 | SRX156334 | SRS347202 | SRP013950 | PRJNA169500 | Danio rerio embryonic promoterome | PRJNA169500 | Transcriptome Analysis | Goal of this study is to generate genome wide maps of transcription initiation throughout early embryonic development of zebrafish Danio rerio. Cap analysis of gene expression CAGE is used to detect transcription start sites at 1bp resolution. CAGE data is complemented by ChIPseq datasets for promoter associated histone modifications to study dynamic changes of promoter usage and chromatin configuration throughout early embryonic development. | pubmed:24531765 | Zebrafish wild type AB strain embryo 64 cells stage | D. rerio 64 cells embryo | D. rerio 64 cells embryo | CAGE D. rerio 64 cells embryo | CAGE D. rerio 64 cells embryo | D. rerio 64 cells embryo | 1 | OTHER | TRANSCRIPTOMIC | CAGE | SINGLE | ILLUMINA | Illumina Genome Analyzer IIx | <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>27</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> | SRP013950 | CAGE_64cells.fastq | fastq | 162784188.0 | 6029044.0 | CAGE D. rerio 64 cells embryo | 0:27 | A:41192860;C:36547869;G:46848833;T:38194626;N:0 | 27 | 41192860 | 36547869 | 46848833 | 38194626 | 0 | SRX156334 | SRS347202 | SRA055273 | University of Bergen | ZEPROME consortium | B | usable mapping rate | illumina | early_illumina | unknown | cage | unknown | bulk | unknown | unknown | Unknown | 2013-08-31 | Cleavage | Embryo | Embryo Imprecise | All anatomical structures | |||||||||||||||||||||||||||||||
| 36423 | 36423 | SRR516547 | SRX156325 | SRS347201 | SRP013950 | PRJNA169500 | Danio rerio embryonic promoterome | PRJNA169500 | Transcriptome Analysis | Goal of this study is to generate genome wide maps of transcription initiation throughout early embryonic development of zebrafish Danio rerio. Cap analysis of gene expression CAGE is used to detect transcription start sites at 1bp resolution. CAGE data is complemented by ChIPseq datasets for promoter associated histone modifications to study dynamic changes of promoter usage and chromatin configuration throughout early embryonic development. | pubmed:24531765 | Zebrafish wild type AB strain fertilized egg | D. rerio fertilized egg | D. rerio fertilized egg | CAGE D. rerio fertilized egg | CAGE D. rerio fertilized egg | D. rerio fertilized egg | 1 | OTHER | TRANSCRIPTOMIC | CAGE | SINGLE | ILLUMINA | Illumina Genome Analyzer IIx | <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>27</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> | SRP013950 | CAGE_fertilized_egg.fastq | fastq | 160948350.0 | 5961050.0 | CAGE D. rerio fertilized egg | 0:27 | A:42030009;C:34700589;G:45399755;T:38817997;N:0 | 27 | 42030009 | 34700589 | 45399755 | 38817997 | 0 | SRX156325 | SRS347201 | SRA055273 | University of Bergen | ZEPROME consortium | 1 | 0.49394 | 0.07554 | 0.81684 | 0.79759 | 27 | B | usable mapping rate | illumina | early_illumina | unknown | cage | unknown | bulk | unknown | unknown | Unknown | 2012-06-28 | Undetermined | Embryo | Undetermined | Embryo Imprecise | |||||||||||||||||||||||||
| 36424 | 36424 | SRR516545 | SRX156307 | SRS347187 | SRP013950 | PRJNA169500 | Danio rerio embryonic promoterome | PRJNA169500 | Transcriptome Analysis | Goal of this study is to generate genome wide maps of transcription initiation throughout early embryonic development of zebrafish Danio rerio. Cap analysis of gene expression CAGE is used to detect transcription start sites at 1bp resolution. CAGE data is complemented by ChIPseq datasets for promoter associated histone modifications to study dynamic changes of promoter usage and chromatin configuration throughout early embryonic development. | pubmed:24531765 | Zebrafish wild type AB strain unfertilized egg | D. rerio unfertilized egg | D. rerio unfertilized egg | CAGE D. rerio unfertilized egg | CAGE D. rerio unfertilized egg run2 | D. rerio unfertilized egg | 1 | OTHER | TRANSCRIPTOMIC | CAGE | SINGLE | ILLUMINA | Illumina Genome Analyzer IIx | <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>36</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> | SRP013950 | 115273233.0 | 4269379.0 | CAGE D. rerio unfertilized egg run1 | 0:27 | A:29909511;C:24823784;G:32519899;T:28020039;N:0 | 27 | 29909511 | 24823784 | 32519899 | 28020039 | 0 | SRX156307 | SRS347187 | SRA055273 | University of Bergen | ZEPROME consortium | 1 | 0.49872 | 0.07655 | 0.81673 | 0.80445 | 27 | B | usable mapping rate | illumina | early_illumina | unknown | cage | unknown | bulk | unknown | unknown | Unknown | 2012-06-28 | Zygote | Embryo | Oocyte | Reproductive System | |||||||||||||||||||||||||||
| 36425 | 36425 | SRR516546 | SRX156307 | SRS347187 | SRP013950 | PRJNA169500 | Danio rerio embryonic promoterome | PRJNA169500 | Transcriptome Analysis | Goal of this study is to generate genome wide maps of transcription initiation throughout early embryonic development of zebrafish Danio rerio. Cap analysis of gene expression CAGE is used to detect transcription start sites at 1bp resolution. CAGE data is complemented by ChIPseq datasets for promoter associated histone modifications to study dynamic changes of promoter usage and chromatin configuration throughout early embryonic development. | pubmed:24531765 | Zebrafish wild type AB strain unfertilized egg | D. rerio unfertilized egg | D. rerio unfertilized egg | CAGE D. rerio unfertilized egg | CAGE D. rerio unfertilized egg run2 | D. rerio unfertilized egg | 1 | OTHER | TRANSCRIPTOMIC | CAGE | SINGLE | ILLUMINA | Illumina Genome Analyzer IIx | <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>36</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> | SRP013950 | 112673943.0 | 4173109.0 | CAGE D. rerio unfertilized egg run2 | 0:27 | A:28315221;C:24199098;G:32599482;T:27560142;N:0 | 27 | 28315221 | 24199098 | 32599482 | 27560142 | 0 | SRX156307 | SRS347187 | SRA055273 | University of Bergen | ZEPROME consortium | 1 | 0.46201 | 0.06422 | 0.82118 | 0.82184 | 27 | B | usable mapping rate | illumina | early_illumina | unknown | cage | unknown | bulk | unknown | unknown | Unknown | 2012-06-28 | Zygote | Embryo | Oocyte | Reproductive System | |||||||||||||||||||||||||||
| 48305 | 48305 | SRR10368285 | SRX7076279 | SRS5592164 | SRP145615 | PRJNA471311 | Gene expression analysis of jam3b mutant embryos | GSE114416 | Transcriptome Analysis | Junctional adhesion molecule 3 Jam3 is a cell adhesion molecule that is involved in cell cell interaction cell migration and cell polarization in many tissue including the epithelium endothelium and hematopoietic cells. Although Jam3 is functionally relevant for both hematopoietic and vascular endothelial cells the role of Jam3 in hematopoietic or vascular development is still unknown. In this study a zebrafish jam3b mutant line jam3b sa37 was used for the cap analysis gene expression CAGE sequencing to identify genes that are involved in hematopoietic and/or vascular development in the zebrafish embryo. Overall design: Whole embryos mRNA profiles of wild type or jam3b sa37 embryos at 12 or 16 hpf were generated by CAGE sequencing using Illumina NextSeq500. | sa37 16hpf 2 | GSM4144779 | source name:whole embryo|tissue:whole embryo|age:16 hpf sa37 | sa37 16hpf 2 | Illumina RTA software was used for base calling. Sequenced CAGE tags were mapped to the full genome sequences for Danio rerio danRer10 using the Burrows Wheeler Aligner BWA and HiSAT2 software. Tags per million TPM were calculated using the CAGEr package of Bioconductor in R ver. 3.3.3 with the minimum TPM value of 0.5. Genome build: danRer10 Supplementary files format and content: Tab delimited text files include tags per million TPM values for each sample. | whole embryo | Total RNAs were extracted from staged embryos using RNeasy Mini Kit QIAGEN. 2?g of total RNAs from embryos were used for synthesis of the cDNA using a Library Preparation kit DNAFORM. five primecaps of RNAs are biotinylated post reverse transcription. RNA cDNA hybirds were then captured by streptavidin conjugated magnetic beads. The cDNAs were released from RNAs and ligated with the 5? linker which includes the three nucleotide barcodes followed by 3? linker ligation. Second strand synthesis was performed using the nAnT iCAGE second primer creating the final double stranded DNA product. Cap analysis gene expression CAGE sequencing | tissue:whole embryo|age:16 hpf sa37 | GSM4144779 | GSM4144779: sa37 16hpf 2; Danio rerio; RNA Seq | GSM4144779 | 1 | Total RNAs were extracted from staged embryos using RNeasy Mini Kit QIAGEN. 2?g of total RNAs from embryos were used for synthesis of the cDNA using a Library Preparation kit DNAFORM. five primecaps of RNAs are biotinylated post reverse transcription. RNA cDNA hybirds were then captured by streptavidin conjugated magnetic beads. The cDNAs were released from RNAs and ligated with the 5? linker which includes the three nucleotide barcodes followed by 3? linker ligation. Second strand synthesis was performed using the nAnT iCAGE second primer creating the final double stranded DNA product. Cap analysis gene expression CAGE sequencing | GEO Accession:GSM4144779 | RNA-Seq | TRANSCRIPTOMIC | CAGE | SINGLE | ILLUMINA | NextSeq 500 | SRP145615 | S04.fastq.gz | fastq | 2166354993.0 | 28701604.0 | GSM4144779 r1 | 0:75.48 1:0 | A:566482594;C:525711117;G:579970160;T:493751194;N:439928 | 75 | 0 | 566482594 | 525711117 | 579970160 | 493751194 | 439928 | SRX7076279 | SRS5592164 | SRA703389 | GEO | Kanazawa University | 1 | 0.92378 | 0.09905 | 0.74479 | 0.52368 | 75 | B | usable mapping rate | illumina | nextseq | unknown | cage | unknown | bulk | unknown | unknown | Japan | 2019-10-30 | Segmentation | Embryo | Whole Organism | All anatomical structures | |||||||||||||||||||
| 48306 | 48306 | SRR10368284 | SRX7076278 | SRS5592163 | SRP145615 | PRJNA471311 | Gene expression analysis of jam3b mutant embryos | GSE114416 | Transcriptome Analysis | Junctional adhesion molecule 3 Jam3 is a cell adhesion molecule that is involved in cell cell interaction cell migration and cell polarization in many tissue including the epithelium endothelium and hematopoietic cells. Although Jam3 is functionally relevant for both hematopoietic and vascular endothelial cells the role of Jam3 in hematopoietic or vascular development is still unknown. In this study a zebrafish jam3b mutant line jam3b sa37 was used for the cap analysis gene expression CAGE sequencing to identify genes that are involved in hematopoietic and/or vascular development in the zebrafish embryo. Overall design: Whole embryos mRNA profiles of wild type or jam3b sa37 embryos at 12 or 16 hpf were generated by CAGE sequencing using Illumina NextSeq500. | sa37 12hpf 2 | GSM4144778 | source name:whole embryo|tissue:whole embryo|age:12 hpf sa37 | sa37 12hpf 2 | Illumina RTA software was used for base calling. Sequenced CAGE tags were mapped to the full genome sequences for Danio rerio danRer10 using the Burrows Wheeler Aligner BWA and HiSAT2 software. Tags per million TPM were calculated using the CAGEr package of Bioconductor in R ver. 3.3.3 with the minimum TPM value of 0.5. Genome build: danRer10 Supplementary files format and content: Tab delimited text files include tags per million TPM values for each sample. | whole embryo | Total RNAs were extracted from staged embryos using RNeasy Mini Kit QIAGEN. 2?g of total RNAs from embryos were used for synthesis of the cDNA using a Library Preparation kit DNAFORM. five primecaps of RNAs are biotinylated post reverse transcription. RNA cDNA hybirds were then captured by streptavidin conjugated magnetic beads. The cDNAs were released from RNAs and ligated with the 5? linker which includes the three nucleotide barcodes followed by 3? linker ligation. Second strand synthesis was performed using the nAnT iCAGE second primer creating the final double stranded DNA product. Cap analysis gene expression CAGE sequencing | tissue:whole embryo|age:12 hpf sa37 | GSM4144778 | GSM4144778: sa37 12hpf 2; Danio rerio; RNA Seq | GSM4144778 | 1 | Total RNAs were extracted from staged embryos using RNeasy Mini Kit QIAGEN. 2?g of total RNAs from embryos were used for synthesis of the cDNA using a Library Preparation kit DNAFORM. five primecaps of RNAs are biotinylated post reverse transcription. RNA cDNA hybirds were then captured by streptavidin conjugated magnetic beads. The cDNAs were released from RNAs and ligated with the 5? linker which includes the three nucleotide barcodes followed by 3? linker ligation. Second strand synthesis was performed using the nAnT iCAGE second primer creating the final double stranded DNA product. Cap analysis gene expression CAGE sequencing | GEO Accession:GSM4144778 | RNA-Seq | TRANSCRIPTOMIC | CAGE | SINGLE | ILLUMINA | NextSeq 500 | SRP145615 | S03.fastq.gz | fastq | 1653073372.0 | 21903605.0 | GSM4144778 r1 | 0:75.47 1:0 | A:433206792;C:402537379;G:446761303;T:370161548;N:406350 | 75 | 0 | 433206792 | 402537379 | 446761303 | 370161548 | 406350 | SRX7076278 | SRS5592163 | SRA703389 | GEO | Kanazawa University | 1 | 0.91778 | 0.12219 | 0.76684 | 0.54487 | 76 | B | usable mapping rate | illumina | nextseq | unknown | cage | unknown | bulk | unknown | unknown | Japan | 2019-10-30 | Segmentation | Embryo | Whole Organism | All anatomical structures | |||||||||||||||||||
| 48307 | 48307 | SRR10368283 | SRX7076277 | SRS5592162 | SRP145615 | PRJNA471311 | Gene expression analysis of jam3b mutant embryos | GSE114416 | Transcriptome Analysis | Junctional adhesion molecule 3 Jam3 is a cell adhesion molecule that is involved in cell cell interaction cell migration and cell polarization in many tissue including the epithelium endothelium and hematopoietic cells. Although Jam3 is functionally relevant for both hematopoietic and vascular endothelial cells the role of Jam3 in hematopoietic or vascular development is still unknown. In this study a zebrafish jam3b mutant line jam3b sa37 was used for the cap analysis gene expression CAGE sequencing to identify genes that are involved in hematopoietic and/or vascular development in the zebrafish embryo. Overall design: Whole embryos mRNA profiles of wild type or jam3b sa37 embryos at 12 or 16 hpf were generated by CAGE sequencing using Illumina NextSeq500. | WT 16hpf 2 | GSM4144777 | source name:whole embryo|tissue:whole embryo|age:16 hpf type | WT 16hpf 2 | Illumina RTA software was used for base calling. Sequenced CAGE tags were mapped to the full genome sequences for Danio rerio danRer10 using the Burrows Wheeler Aligner BWA and HiSAT2 software. Tags per million TPM were calculated using the CAGEr package of Bioconductor in R ver. 3.3.3 with the minimum TPM value of 0.5. Genome build: danRer10 Supplementary files format and content: Tab delimited text files include tags per million TPM values for each sample. | whole embryo | Total RNAs were extracted from staged embryos using RNeasy Mini Kit QIAGEN. 2?g of total RNAs from embryos were used for synthesis of the cDNA using a Library Preparation kit DNAFORM. five primecaps of RNAs are biotinylated post reverse transcription. RNA cDNA hybirds were then captured by streptavidin conjugated magnetic beads. The cDNAs were released from RNAs and ligated with the 5? linker which includes the three nucleotide barcodes followed by 3? linker ligation. Second strand synthesis was performed using the nAnT iCAGE second primer creating the final double stranded DNA product. Cap analysis gene expression CAGE sequencing | tissue:whole embryo|age:16 hpf type | GSM4144777 | GSM4144777: WT 16hpf 2; Danio rerio; RNA Seq | GSM4144777 | 1 | Total RNAs were extracted from staged embryos using RNeasy Mini Kit QIAGEN. 2?g of total RNAs from embryos were used for synthesis of the cDNA using a Library Preparation kit DNAFORM. five primecaps of RNAs are biotinylated post reverse transcription. RNA cDNA hybirds were then captured by streptavidin conjugated magnetic beads. The cDNAs were released from RNAs and ligated with the 5? linker which includes the three nucleotide barcodes followed by 3? linker ligation. Second strand synthesis was performed using the nAnT iCAGE second primer creating the final double stranded DNA product. Cap analysis gene expression CAGE sequencing | GEO Accession:GSM4144777 | RNA-Seq | TRANSCRIPTOMIC | CAGE | SINGLE | ILLUMINA | NextSeq 500 | SRP145615 | S02.fastq.gz | fastq | 2178611783.0 | 28900952.0 | GSM4144777 r1 | 0:75.38 1:0 | A:566689783;C:530433539;G:590611812;T:488215395;N:2661254 | 75 | 0 | 566689783 | 530433539 | 590611812 | 488215395 | 2661254 | SRX7076277 | SRS5592162 | SRA703389 | GEO | Kanazawa University | 1 | 0.93637 | 0.10293 | 0.7542 | 0.53437 | 76 | B | usable mapping rate | illumina | nextseq | unknown | cage | unknown | bulk | unknown | unknown | Japan | 2019-10-30 | Segmentation | Embryo | Whole Organism | All anatomical structures | |||||||||||||||||||
| 48308 | 48308 | SRR10368282 | SRX7076276 | SRS5592161 | SRP145615 | PRJNA471311 | Gene expression analysis of jam3b mutant embryos | GSE114416 | Transcriptome Analysis | Junctional adhesion molecule 3 Jam3 is a cell adhesion molecule that is involved in cell cell interaction cell migration and cell polarization in many tissue including the epithelium endothelium and hematopoietic cells. Although Jam3 is functionally relevant for both hematopoietic and vascular endothelial cells the role of Jam3 in hematopoietic or vascular development is still unknown. In this study a zebrafish jam3b mutant line jam3b sa37 was used for the cap analysis gene expression CAGE sequencing to identify genes that are involved in hematopoietic and/or vascular development in the zebrafish embryo. Overall design: Whole embryos mRNA profiles of wild type or jam3b sa37 embryos at 12 or 16 hpf were generated by CAGE sequencing using Illumina NextSeq500. | WT 12hpf 2 | GSM4144776 | source name:whole embryo|tissue:whole embryo|age:12 hpf type | WT 12hpf 2 | Illumina RTA software was used for base calling. Sequenced CAGE tags were mapped to the full genome sequences for Danio rerio danRer10 using the Burrows Wheeler Aligner BWA and HiSAT2 software. Tags per million TPM were calculated using the CAGEr package of Bioconductor in R ver. 3.3.3 with the minimum TPM value of 0.5. Genome build: danRer10 Supplementary files format and content: Tab delimited text files include tags per million TPM values for each sample. | whole embryo | Total RNAs were extracted from staged embryos using RNeasy Mini Kit QIAGEN. 2?g of total RNAs from embryos were used for synthesis of the cDNA using a Library Preparation kit DNAFORM. five primecaps of RNAs are biotinylated post reverse transcription. RNA cDNA hybirds were then captured by streptavidin conjugated magnetic beads. The cDNAs were released from RNAs and ligated with the 5? linker which includes the three nucleotide barcodes followed by 3? linker ligation. Second strand synthesis was performed using the nAnT iCAGE second primer creating the final double stranded DNA product. Cap analysis gene expression CAGE sequencing | tissue:whole embryo|age:12 hpf type | GSM4144776 | GSM4144776: WT 12hpf 2; Danio rerio; RNA Seq | GSM4144776 | 1 | Total RNAs were extracted from staged embryos using RNeasy Mini Kit QIAGEN. 2?g of total RNAs from embryos were used for synthesis of the cDNA using a Library Preparation kit DNAFORM. five primecaps of RNAs are biotinylated post reverse transcription. RNA cDNA hybirds were then captured by streptavidin conjugated magnetic beads. The cDNAs were released from RNAs and ligated with the 5? linker which includes the three nucleotide barcodes followed by 3? linker ligation. Second strand synthesis was performed using the nAnT iCAGE second primer creating the final double stranded DNA product. Cap analysis gene expression CAGE sequencing | GEO Accession:GSM4144776 | RNA-Seq | TRANSCRIPTOMIC | CAGE | SINGLE | ILLUMINA | NextSeq 500 | SRP145615 | S01.fastq.gz | fastq | 2235469804.0 | 29611055.0 | GSM4144776 r1 | 0:75.49 1:0 | A:582276989;C:546056334;G:609905170;T:497018748;N:212563 | 75 | 0 | 582276989 | 546056334 | 609905170 | 497018748 | 212563 | SRX7076276 | SRS5592161 | SRA703389 | GEO | Kanazawa University | 1 | 0.9362 | 0.12401 | 0.76857 | 0.48408 | 76 | B | usable mapping rate | illumina | nextseq | unknown | cage | unknown | bulk | unknown | unknown | Japan | 2019-10-30 | Segmentation | Embryo | Whole Organism | All anatomical structures | |||||||||||||||||||
| 48309 | 48309 | SRR7162665 | SRX4080963 | SRS3298238 | SRP145615 | PRJNA471311 | Gene expression analysis of jam3b mutant embryos | GSE114416 | Transcriptome Analysis | Junctional adhesion molecule 3 Jam3 is a cell adhesion molecule that is involved in cell cell interaction cell migration and cell polarization in many tissue including the epithelium endothelium and hematopoietic cells. Although Jam3 is functionally relevant for both hematopoietic and vascular endothelial cells the role of Jam3 in hematopoietic or vascular development is still unknown. In this study a zebrafish jam3b mutant line jam3b sa37 was used for the cap analysis gene expression CAGE sequencing to identify genes that are involved in hematopoietic and/or vascular development in the zebrafish embryo. Overall design: Whole embryos mRNA profiles of wild type or jam3b sa37 embryos at 12 or 16 hpf were generated by CAGE sequencing using Illumina NextSeq500. | sa37 16hpf | GSM3141418 | source name:whole embryo|tissue:whole embryo|age:16 hpf sa37 | sa37 16hpf | Illumina RTA software was used for base calling. Sequenced CAGE tags were mapped to the full genome sequences for Danio rerio danRer10 using the Burrows Wheeler Aligner BWA and HiSAT2 software. Tags per million TPM were calculated using the CAGEr package of Bioconductor in R ver. 3.3.3 with the minimum TPM value of 0.5. Genome build: danRer10 Supplementary files format and content: Tab delimited text files include tags per million TPM values for each sample. | whole embryo | Total RNAs were extracted from staged embryos using RNeasy Mini Kit QIAGEN. 2μg of total RNAs from embryos were used for synthesis of the cDNA using a Library Preparation kit DNAFORM. five primecaps of RNAs are biotinylated post reverse transcription. RNA cDNA hybirds were then captured by streptavidin conjugated magnetic beads. The cDNAs were released from RNAs and ligated with the 5′ linker which includes the three nucleotide barcodes followed by 3′ linker ligation. Second strand synthesis was performed using the nAnT iCAGE second primer creating the final double stranded DNA product. Cap analysis gene expression CAGE sequencing | tissue:whole embryo|age:16 hpf sa37 | GSM3141418 | GSM3141418: sa37 16hpf; Danio rerio; RNA Seq | GSM3141418 | 1 | Total RNAs were extracted from staged embryos using RNeasy Mini Kit QIAGEN. 2μg of total RNAs from embryos were used for synthesis of the cDNA using a Library Preparation kit DNAFORM. five primecaps of RNAs are biotinylated post reverse transcription. RNA cDNA hybirds were then captured by streptavidin conjugated magnetic beads. The cDNAs were released from RNAs and ligated with the 5′ linker which includes the three nucleotide barcodes followed by 3′ linker ligation. Second strand synthesis was performed using the nAnT iCAGE second primer creating the final double stranded DNA product. Cap analysis gene expression CAGE sequencing | GEO Accession:GSM3141418 | RNA-Seq | TRANSCRIPTOMIC | CAGE | SINGLE | ILLUMINA | NextSeq 500 | SRP145615 | KB4.fastq.gz | fastq | 1534816794.0 | 20351965.0 | GSM3141418 r1 | 0:75.41 1:0 | A:406883394;C:371384442;G:408553824;T:347148313;N:846821 | 75 | 0 | 406883394 | 371384442 | 408553824 | 347148313 | 846821 | SRX4080963 | SRS3298238 | SRA703389 | GEO | Kanazawa University | 1 | 0.90303 | 0.11364 | 0.7726 | 0.54943 | 76 | B | usable mapping rate | illumina | nextseq | unknown | cage | unknown | bulk | unknown | unknown | Japan | 2018-05-14 | Segmentation | Embryo | Whole Organism | All anatomical structures | |||||||||||||||||||
| 48310 | 48310 | SRR7162664 | SRX4080962 | SRS3298237 | SRP145615 | PRJNA471311 | Gene expression analysis of jam3b mutant embryos | GSE114416 | Transcriptome Analysis | Junctional adhesion molecule 3 Jam3 is a cell adhesion molecule that is involved in cell cell interaction cell migration and cell polarization in many tissue including the epithelium endothelium and hematopoietic cells. Although Jam3 is functionally relevant for both hematopoietic and vascular endothelial cells the role of Jam3 in hematopoietic or vascular development is still unknown. In this study a zebrafish jam3b mutant line jam3b sa37 was used for the cap analysis gene expression CAGE sequencing to identify genes that are involved in hematopoietic and/or vascular development in the zebrafish embryo. Overall design: Whole embryos mRNA profiles of wild type or jam3b sa37 embryos at 12 or 16 hpf were generated by CAGE sequencing using Illumina NextSeq500. | sa37 12hpf | GSM3141417 | source name:whole embryo|tissue:whole embryo|age:12 hpf sa37 | sa37 12hpf | Illumina RTA software was used for base calling. Sequenced CAGE tags were mapped to the full genome sequences for Danio rerio danRer10 using the Burrows Wheeler Aligner BWA and HiSAT2 software. Tags per million TPM were calculated using the CAGEr package of Bioconductor in R ver. 3.3.3 with the minimum TPM value of 0.5. Genome build: danRer10 Supplementary files format and content: Tab delimited text files include tags per million TPM values for each sample. | whole embryo | Total RNAs were extracted from staged embryos using RNeasy Mini Kit QIAGEN. 2μg of total RNAs from embryos were used for synthesis of the cDNA using a Library Preparation kit DNAFORM. five primecaps of RNAs are biotinylated post reverse transcription. RNA cDNA hybirds were then captured by streptavidin conjugated magnetic beads. The cDNAs were released from RNAs and ligated with the 5′ linker which includes the three nucleotide barcodes followed by 3′ linker ligation. Second strand synthesis was performed using the nAnT iCAGE second primer creating the final double stranded DNA product. Cap analysis gene expression CAGE sequencing | tissue:whole embryo|age:12 hpf sa37 | GSM3141417 | GSM3141417: sa37 12hpf; Danio rerio; RNA Seq | GSM3141417 | 1 | Total RNAs were extracted from staged embryos using RNeasy Mini Kit QIAGEN. 2μg of total RNAs from embryos were used for synthesis of the cDNA using a Library Preparation kit DNAFORM. five primecaps of RNAs are biotinylated post reverse transcription. RNA cDNA hybirds were then captured by streptavidin conjugated magnetic beads. The cDNAs were released from RNAs and ligated with the 5′ linker which includes the three nucleotide barcodes followed by 3′ linker ligation. Second strand synthesis was performed using the nAnT iCAGE second primer creating the final double stranded DNA product. Cap analysis gene expression CAGE sequencing | GEO Accession:GSM3141417 | RNA-Seq | TRANSCRIPTOMIC | CAGE | SINGLE | ILLUMINA | NextSeq 500 | SRP145615 | KB3.fastq.gz | fastq | 1102623174.0 | 14620872.0 | GSM3141417 r1 | 0:75.41 1:0 | A:294837961;C:265832591;G:295010626;T:246478308;N:463688 | 75 | 0 | 294837961 | 265832591 | 295010626 | 246478308 | 463688 | SRX4080962 | SRS3298237 | SRA703389 | GEO | Kanazawa University | 1 | 0.89407 | 0.145 | 0.79299 | 0.58846 | 76 | B | usable mapping rate | illumina | nextseq | unknown | cage | unknown | bulk | unknown | unknown | Japan | 2018-05-14 | Segmentation | Embryo | Whole Organism | All anatomical structures | |||||||||||||||||||
| 48311 | 48311 | SRR7162663 | SRX4080961 | SRS3298236 | SRP145615 | PRJNA471311 | Gene expression analysis of jam3b mutant embryos | GSE114416 | Transcriptome Analysis | Junctional adhesion molecule 3 Jam3 is a cell adhesion molecule that is involved in cell cell interaction cell migration and cell polarization in many tissue including the epithelium endothelium and hematopoietic cells. Although Jam3 is functionally relevant for both hematopoietic and vascular endothelial cells the role of Jam3 in hematopoietic or vascular development is still unknown. In this study a zebrafish jam3b mutant line jam3b sa37 was used for the cap analysis gene expression CAGE sequencing to identify genes that are involved in hematopoietic and/or vascular development in the zebrafish embryo. Overall design: Whole embryos mRNA profiles of wild type or jam3b sa37 embryos at 12 or 16 hpf were generated by CAGE sequencing using Illumina NextSeq500. | WT 16hpf | GSM3141416 | source name:whole embryo|tissue:whole embryo|age:16 hpf type | WT 16hpf | Illumina RTA software was used for base calling. Sequenced CAGE tags were mapped to the full genome sequences for Danio rerio danRer10 using the Burrows Wheeler Aligner BWA and HiSAT2 software. Tags per million TPM were calculated using the CAGEr package of Bioconductor in R ver. 3.3.3 with the minimum TPM value of 0.5. Genome build: danRer10 Supplementary files format and content: Tab delimited text files include tags per million TPM values for each sample. | whole embryo | Total RNAs were extracted from staged embryos using RNeasy Mini Kit QIAGEN. 2μg of total RNAs from embryos were used for synthesis of the cDNA using a Library Preparation kit DNAFORM. five primecaps of RNAs are biotinylated post reverse transcription. RNA cDNA hybirds were then captured by streptavidin conjugated magnetic beads. The cDNAs were released from RNAs and ligated with the 5′ linker which includes the three nucleotide barcodes followed by 3′ linker ligation. Second strand synthesis was performed using the nAnT iCAGE second primer creating the final double stranded DNA product. Cap analysis gene expression CAGE sequencing | tissue:whole embryo|age:16 hpf type | GSM3141416 | GSM3141416: WT 16hpf; Danio rerio; RNA Seq | GSM3141416 | 1 | Total RNAs were extracted from staged embryos using RNeasy Mini Kit QIAGEN. 2μg of total RNAs from embryos were used for synthesis of the cDNA using a Library Preparation kit DNAFORM. five primecaps of RNAs are biotinylated post reverse transcription. RNA cDNA hybirds were then captured by streptavidin conjugated magnetic beads. The cDNAs were released from RNAs and ligated with the 5′ linker which includes the three nucleotide barcodes followed by 3′ linker ligation. Second strand synthesis was performed using the nAnT iCAGE second primer creating the final double stranded DNA product. Cap analysis gene expression CAGE sequencing | GEO Accession:GSM3141416 | RNA-Seq | TRANSCRIPTOMIC | CAGE | SINGLE | ILLUMINA | NextSeq 500 | SRP145615 | KB2.fastq.gz | fastq | 1436366901.0 | 19049708.0 | GSM3141416 r1 | 0:75.40 1:0 | A:385845952;C:344690132;G:380040548;T:324881238;N:909031 | 75 | 0 | 385845952 | 344690132 | 380040548 | 324881238 | 909031 | SRX4080961 | SRS3298236 | SRA703389 | GEO | Kanazawa University | 1 | 0.90003 | 0.11916 | 0.77579 | 0.54897 | 75 | B | usable mapping rate | illumina | nextseq | unknown | cage | unknown | bulk | unknown | unknown | Japan | 2018-05-14 | Segmentation | Embryo | Whole Organism | All anatomical structures | |||||||||||||||||||
| 48312 | 48312 | SRR7162662 | SRX4080960 | SRS3298235 | SRP145615 | PRJNA471311 | Gene expression analysis of jam3b mutant embryos | GSE114416 | Transcriptome Analysis | Junctional adhesion molecule 3 Jam3 is a cell adhesion molecule that is involved in cell cell interaction cell migration and cell polarization in many tissue including the epithelium endothelium and hematopoietic cells. Although Jam3 is functionally relevant for both hematopoietic and vascular endothelial cells the role of Jam3 in hematopoietic or vascular development is still unknown. In this study a zebrafish jam3b mutant line jam3b sa37 was used for the cap analysis gene expression CAGE sequencing to identify genes that are involved in hematopoietic and/or vascular development in the zebrafish embryo. Overall design: Whole embryos mRNA profiles of wild type or jam3b sa37 embryos at 12 or 16 hpf were generated by CAGE sequencing using Illumina NextSeq500. | WT 12hpf | GSM3141415 | source name:whole embryo|tissue:whole embryo|age:12 hpf type | WT 12hpf | Illumina RTA software was used for base calling. Sequenced CAGE tags were mapped to the full genome sequences for Danio rerio danRer10 using the Burrows Wheeler Aligner BWA and HiSAT2 software. Tags per million TPM were calculated using the CAGEr package of Bioconductor in R ver. 3.3.3 with the minimum TPM value of 0.5. Genome build: danRer10 Supplementary files format and content: Tab delimited text files include tags per million TPM values for each sample. | whole embryo | Total RNAs were extracted from staged embryos using RNeasy Mini Kit QIAGEN. 2μg of total RNAs from embryos were used for synthesis of the cDNA using a Library Preparation kit DNAFORM. five primecaps of RNAs are biotinylated post reverse transcription. RNA cDNA hybirds were then captured by streptavidin conjugated magnetic beads. The cDNAs were released from RNAs and ligated with the 5′ linker which includes the three nucleotide barcodes followed by 3′ linker ligation. Second strand synthesis was performed using the nAnT iCAGE second primer creating the final double stranded DNA product. Cap analysis gene expression CAGE sequencing | tissue:whole embryo|age:12 hpf type | GSM3141415 | GSM3141415: WT 12hpf; Danio rerio; RNA Seq | GSM3141415 | 1 | Total RNAs were extracted from staged embryos using RNeasy Mini Kit QIAGEN. 2μg of total RNAs from embryos were used for synthesis of the cDNA using a Library Preparation kit DNAFORM. five primecaps of RNAs are biotinylated post reverse transcription. RNA cDNA hybirds were then captured by streptavidin conjugated magnetic beads. The cDNAs were released from RNAs and ligated with the 5′ linker which includes the three nucleotide barcodes followed by 3′ linker ligation. Second strand synthesis was performed using the nAnT iCAGE second primer creating the final double stranded DNA product. Cap analysis gene expression CAGE sequencing | GEO Accession:GSM3141415 | RNA-Seq | TRANSCRIPTOMIC | CAGE | SINGLE | ILLUMINA | NextSeq 500 | SRP145615 | KB1.fastq.gz | fastq | 1207735359.0 | 16013304.0 | GSM3141415 r1 | 0:75.42 1:0 | A:319723192;C:291608829;G:328515002;T:267246909;N:641427 | 75 | 0 | 319723192 | 291608829 | 328515002 | 267246909 | 641427 | SRX4080960 | SRS3298235 | SRA703389 | GEO | Kanazawa University | 1 | 0.90112 | 0.14655 | 0.79762 | 0.52207 | 76 | B | usable mapping rate | illumina | nextseq | unknown | cage | unknown | bulk | unknown | unknown | Japan | 2018-05-14 | Segmentation | Embryo | Whole Organism | All anatomical structures | |||||||||||||||||||
| 55266 | 55266 | SRR10418684 | SRX7116713 | SRS5465204 | SRP223930 | PRJNA575342 | CAGE /CappedRNA sequencig | PRJNA575342 | Other | CAGE and full length capped RNA sequencing for identification of transcription start TSS utilisation during Zebrafish Danio rerio embryonic development | S06 Prim5 | strain:AB|dev stage:Prim 5|sex:N/A|tissue:whole embryo|BioSampleModel:Model organism or animal | CAGE seq of Danio rerio whole embryo Prim 5 stage Cycloheximide treated for 2 hours | Prim5 tagCAGE CHX | Prim5 tagCAGE CHX | Tagging CAGE | RNA-Seq | TRANSCRIPTOMIC | CAGE | SINGLE | ILLUMINA | Illumina Genome Analyzer IIx | SRP223930 | S06_Prim5_tagCAGE-CHX.fastq.gz | fastq | 272077515.0 | 10076945.0 | S06 Prim5 tagCAGE CHX.fastq.gz | 0:27 | A:67002738;C:62164745;G:73707100;T:69202932;N:0 | 27 | 67002738 | 62164745 | 73707100 | 69202932 | 0 | SRX7116713 | SRS5465204 | SRA993027 | University of Birmingham|Cancer and Genomic Sciences | University of Birmingham | 1 | 0.44237 | 0.05018 | 0.80296 | 0.84125 | 27 | B | usable mapping rate | illumina | early_illumina | full_length | cage | unknown | bulk | unknown | unknown | United Kingdom | 2019-11-08 | Pharyngula | Embryo | Whole Organism | All anatomical structures | ||||||||||||||||||||||||||||
| 55267 | 55267 | SRR10418685 | SRX7116712 | SRS5465204 | SRP223930 | PRJNA575342 | CAGE /CappedRNA sequencig | PRJNA575342 | Other | CAGE and full length capped RNA sequencing for identification of transcription start TSS utilisation during Zebrafish Danio rerio embryonic development | S06 Prim5 | strain:AB|dev stage:Prim 5|sex:N/A|tissue:whole embryo|BioSampleModel:Model organism or animal | CAGE seq of Danio rerio whole embryo Prim 5 stage DMSO control | Prim5 tagCAGE DMSO | Prim5 tagCAGE DMSO | Tagging CAGE | RNA-Seq | TRANSCRIPTOMIC | CAGE | SINGLE | ILLUMINA | Illumina Genome Analyzer IIx | SRP223930 | S06_Prim5_tagCAGE-DMSO.fastq.gz | fastq | 339322419.0 | 12567497.0 | S06 Prim5 tagCAGE DMSO.fastq.gz | 0:27 | A:81884992;C:78138597;G:89012923;T:90285907;N:0 | 27 | 81884992 | 78138597 | 89012923 | 90285907 | 0 | SRX7116712 | SRS5465204 | SRA993027 | University of Birmingham|Cancer and Genomic Sciences | University of Birmingham | 1 | 0.43876 | 0.05032 | 0.82404 | 0.85765 | 27 | B | usable mapping rate | illumina | early_illumina | full_length | cage | unknown | bulk | unknown | unknown | United Kingdom | 2019-11-08 | Pharyngula | Embryo | Whole Organism | All anatomical structures | ||||||||||||||||||||||||||||
| 55269 | 55269 | SRR10215485 | SRX6935170 | SRS5465205 | SRP223930 | PRJNA575342 | CAGE /CappedRNA sequencig | PRJNA575342 | Other | CAGE and full length capped RNA sequencing for identification of transcription start TSS utilisation during Zebrafish Danio rerio embryonic development | S07 LongPec | strain:AB|dev stage:Long pec|sex:N/A|tissue:whole embryo|BioSampleModel:Model organism or animal | CAGE seq of Danio rerio whole embryo Long pec stage | LongPec nAnTiCAGE | LongPec nAnTiCAGE | nAnTiCAGE | RNA-Seq | TRANSCRIPTOMIC | CAGE | SINGLE | ILLUMINA | NextSeq 500 | SRP223930 | S07_LongPec_nAnTiCAGE.fastq.gz | fastq | 1326115656.0 | 18418273.0 | S07 LongPec nAnTiCAGE.fastq.gz | 0:72 1:0 | A:355337919;C:317217156;G:362577018;T:290983563;N:0 | 72 | 0 | 355337919 | 317217156 | 362577018 | 290983563 | 0 | SRX6935170 | SRS5465205 | SRA971223 | University of Birmingham|Cancer and Genomic Sciences | University of Birmingham | 1 | 0.94675 | 0.13499 | 0.77512 | 0.56663 | 72 | B | usable mapping rate | illumina | nextseq | full_length | cage | unknown | bulk | unknown | unknown | United Kingdom | 2019-10-02 | Hatching | Embryo | Whole Organism | All anatomical structures | |||||||||||||||||||||||||||
| 55272 | 55272 | SRR10215488 | SRX6935167 | SRS5465202 | SRP223930 | PRJNA575342 | CAGE /CappedRNA sequencig | PRJNA575342 | Other | CAGE and full length capped RNA sequencing for identification of transcription start TSS utilisation during Zebrafish Danio rerio embryonic development | S04 30Epi | strain:AB|dev stage:zfs:0000015|sex:N/A|tissue:whole embryo|BioSampleModel:Model organism or animal | CAGE seq of Danio rerio whole embryo zfs:0000015 stage | zfs:0000015 nAnTiCAGE | zfs:0000015 nAnTiCAGE | nAnTiCAGE | RNA-Seq | TRANSCRIPTOMIC | CAGE | SINGLE | ILLUMINA | Illumina HiSeq 2500 | SRP223930 | S04_30pEpib_nAnTiCAGE.fastq.gz | fastq | 629440272.0 | 13113339.0 | S04 30pEpib nAnTiCAGE.fastq.gz | 0:48 1:0 | A:170201862;C:150343558;G:166046900;T:141987430;N:860522 | 48 | 0 | 170201862 | 150343558 | 166046900 | 141987430 | 860522 | SRX6935167 | SRS5465202 | SRA971223 | University of Birmingham|Cancer and Genomic Sciences | University of Birmingham | 1 | 0.83843 | 0.15935 | 0.81499 | 0.71304 | 48 | B | usable mapping rate | illumina | hiseq_era | full_length | cage | unknown | bulk | unknown | unknown | United Kingdom | 2019-10-02 | Blastula | Embryo | Whole Organism | All anatomical structures | |||||||||||||||||||||||||||
| 55273 | 55273 | SRR10215489 | SRX6935166 | SRS5465201 | SRP223930 | PRJNA575342 | CAGE /CappedRNA sequencig | PRJNA575342 | Other | CAGE and full length capped RNA sequencing for identification of transcription start TSS utilisation during Zebrafish Danio rerio embryonic development | S03 512Cells | strain:AB|dev stage:512 cell|sex:N/A|tissue:whole embryo|BioSampleModel:Model organism or animal | CAGE seq of Danio rerio whole embryo 512 cell stage | 512Cells nAnTiCAGE | 512Cells nAnTiCAGE | nAnTiCAGE | RNA-Seq | TRANSCRIPTOMIC | CAGE | SINGLE | ILLUMINA | Illumina HiSeq 2500 | SRP223930 | S03_512Cells_nAnTiCAGE.fastq.gz | fastq | 725783040.0 | 15120480.0 | S03 512Cells nAnTiCAGE.fastq.gz | 0:48 1:0 | A:196224130;C:171017037;G:189882541;T:167660445;N:998887 | 48 | 0 | 196224130 | 171017037 | 189882541 | 167660445 | 998887 | SRX6935166 | SRS5465201 | SRA971223 | University of Birmingham|Cancer and Genomic Sciences | University of Birmingham | 1 | 0.85441 | 0.14832 | 0.79659 | 0.68016 | 48 | B | usable mapping rate | illumina | hiseq_era | full_length | cage | unknown | bulk | unknown | unknown | United Kingdom | 2019-10-02 | Blastula | Embryo | Whole Organism | All anatomical structures | |||||||||||||||||||||||||||
| 55274 | 55274 | SRR10215490 | SRX6935165 | SRS5465200 | SRP223930 | PRJNA575342 | CAGE /CappedRNA sequencig | PRJNA575342 | Other | CAGE and full length capped RNA sequencing for identification of transcription start TSS utilisation during Zebrafish Danio rerio embryonic development | S02 128Cells | strain:AB|dev stage:128 cell|sex:N/A|tissue:whole embryo|BioSampleModel:Model organism or animal | CAGE seq of Danio rerio whole embryo 128 cell stage | 128Cells nAnTiCAGE | 128Cells nAnTiCAGE | nAnTiCAGE | RNA-Seq | TRANSCRIPTOMIC | CAGE | SINGLE | ILLUMINA | Illumina HiSeq 2500 | SRP223930 | S02_128Cells_nAnTiCAGE.fastq.gz | fastq | 510549408.0 | 10636446.0 | S02 128Cells nAnTiCAGE.fastq.gz | 0:48 1:0 | A:135389960;C:121538362;G:140642547;T:112273195;N:705344 | 48 | 0 | 135389960 | 121538362 | 140642547 | 112273195 | 705344 | SRX6935165 | SRS5465200 | SRA971223 | University of Birmingham|Cancer and Genomic Sciences | University of Birmingham | 1 | 0.84843 | 0.14523 | 0.79845 | 0.68592 | 48 | B | usable mapping rate | illumina | hiseq_era | full_length | cage | unknown | bulk | unknown | unknown | United Kingdom | 2019-10-02 | Blastula | Embryo | Whole Organism | All anatomical structures | |||||||||||||||||||||||||||
| 69378 | 69378 | SRR18516702 | SRX14647801 | SRS12413210 | SRP366491 | PRJNA821088 | Enhancer calling in early stages | PRJNA821088 | Other | CAGE seq for enhancer calling in early stages for the DANIO CODE project. | DCD007475BS | strain:WT|age:4.7hpf|dev stage:zfs:0000015|sex:not applicable|tissue:early embryonic cell|biomaterial provider:Mueller lab University of Birmingham|replicate:2|BioSampleModel:Model organism or animal | CAGE seq zfs:0000015 Whole cell CAGE | DCD003740SQ | DCD003740SQ | max read length:50 | RNA-Seq | TRANSCRIPTOMIC | CAGE | SINGLE | ILLUMINA | Illumina HiSeq 2500 | SRP366491 | CAGE-seq_Mueller_lab_0013AS.DCD003740SQ.USERdanio-user.R1.fastq.gz | fastq | 57981552.0 | 1207949.0 | CAGE seq Mueller lab 0013AS.DCD003740SQ.USERdanio user.R1.fastq.gz | 0:48 1:0 | A:13228227;C:15492921;G:15505739;T:13750626;N:4039 | 48 | 0 | 13228227 | 15492921 | 15505739 | 13750626 | 4039 | SRX14647801 | SRS12413210 | SRA1393740 | DANIO-CODE|Department for Biosciences and Nutrition | DANIO-CODE DANIO-CODE | 1 | 0.18388 | 0.02619 | 0.85811 | 0.52264 | 48 | B | usable mapping rate | illumina | hiseq_era | unknown | cage | unknown | bulk | unknown | unknown | Unknown | 2022-03-29 | Blastula | Embryo | Embryo Imprecise | All anatomical structures | |||||||||||||||||||||||||||
| 69379 | 69379 | SRR18516703 | SRX14647800 | SRS12413216 | SRP366491 | PRJNA821088 | Enhancer calling in early stages | PRJNA821088 | Other | CAGE seq for enhancer calling in early stages for the DANIO CODE project. | DCD007476BS | strain:WT|age:24hpf|dev stage:Prim 5|sex:not applicable|tissue:early embryonic cell|biomaterial provider:Mueller lab University of Birmingham|replicate:1|BioSampleModel:Model organism or animal | CAGE seq Prim Nuclear CAGE | DCD003719SQ | DCD003719SQ | max read length:50 | RNA-Seq | TRANSCRIPTOMIC | CAGE | SINGLE | ILLUMINA | Illumina HiSeq 2500 | SRP366491 | CAGE-seq_Mueller_lab_0014AS.DCD003719SQ.USERdanio-user.R1.fastq.gz | fastq | 585510192.0 | 12198129.0 | CAGE seq Mueller lab 0014AS.DCD003719SQ.USERdanio user.R1.fastq.gz | 0:48 1:0 | A:129885703;C:153766182;G:160138903;T:141666883;N:52521 | 48 | 0 | 129885703 | 153766182 | 160138903 | 141666883 | 52521 | SRX14647800 | SRS12413216 | SRA1393740 | DANIO-CODE|Department for Biosciences and Nutrition | DANIO-CODE DANIO-CODE | 1 | 0.20391 | 0.04196 | 0.83453 | 0.4979 | 48 | B | usable mapping rate | illumina | hiseq_era | unknown | cage | unknown | bulk | unknown | unknown | Unknown | 2022-03-29 | Pharyngula | Embryo | Embryo Imprecise | All anatomical structures | |||||||||||||||||||||||||||
| 69380 | 69380 | SRR18516704 | SRX14647799 | SRS12413216 | SRP366491 | PRJNA821088 | Enhancer calling in early stages | PRJNA821088 | Other | CAGE seq for enhancer calling in early stages for the DANIO CODE project. | DCD007476BS | strain:WT|age:24hpf|dev stage:Prim 5|sex:not applicable|tissue:early embryonic cell|biomaterial provider:Mueller lab University of Birmingham|replicate:1|BioSampleModel:Model organism or animal | CAGE seq Prim Nuclear CAGE | DCD003718SQ | DCD003718SQ | max read length:50 | RNA-Seq | TRANSCRIPTOMIC | CAGE | SINGLE | ILLUMINA | Illumina HiSeq 2500 | SRP366491 | CAGE-seq_Mueller_lab_0014AS.DCD003718SQ.USERdanio-user.R1.fastq.gz | fastq | 593136528.0 | 12357011.0 | CAGE seq Mueller lab 0014AS.DCD003718SQ.USERdanio user.R1.fastq.gz | 0:48 1:0 | A:130366174;C:156810351;G:159219365;T:146691922;N:48716 | 48 | 0 | 130366174 | 156810351 | 159219365 | 146691922 | 48716 | SRX14647799 | SRS12413216 | SRA1393740 | DANIO-CODE|Department for Biosciences and Nutrition | DANIO-CODE DANIO-CODE | 1 | 0.14873 | 0.03239 | 0.86557 | 0.49634 | 48 | B | usable mapping rate | illumina | hiseq_era | unknown | cage | unknown | bulk | unknown | unknown | Unknown | 2022-03-29 | Pharyngula | Embryo | Embryo Imprecise | All anatomical structures | |||||||||||||||||||||||||||
| 69381 | 69381 | SRR18516705 | SRX14647798 | SRS12413216 | SRP366491 | PRJNA821088 | Enhancer calling in early stages | PRJNA821088 | Other | CAGE seq for enhancer calling in early stages for the DANIO CODE project. | DCD007476BS | strain:WT|age:24hpf|dev stage:Prim 5|sex:not applicable|tissue:early embryonic cell|biomaterial provider:Mueller lab University of Birmingham|replicate:1|BioSampleModel:Model organism or animal | CAGE seq Prim Nuclear CAGE | DCD003723SQ | DCD003723SQ | max read length:50 | RNA-Seq | TRANSCRIPTOMIC | CAGE | SINGLE | ILLUMINA | Illumina HiSeq 2500 | SRP366491 | CAGE-seq_Mueller_lab_0014AS.DCD003723SQ.USERdanio-user.R1.fastq.gz | fastq | 422696256.0 | 8806172.0 | CAGE seq Mueller lab 0014AS.DCD003723SQ.USERdanio user.R1.fastq.gz | 0:48 1:0 | A:92712632;C:111696999;G:115145846;T:103105611;N:35168 | 48 | 0 | 92712632 | 111696999 | 115145846 | 103105611 | 35168 | SRX14647798 | SRS12413216 | SRA1393740 | DANIO-CODE|Department for Biosciences and Nutrition | DANIO-CODE DANIO-CODE | 1 | 0.1594 | 0.02997 | 0.85591 | 0.48496 | 48 | B | usable mapping rate | illumina | hiseq_era | unknown | cage | unknown | bulk | unknown | unknown | Unknown | 2022-03-29 | Pharyngula | Embryo | Embryo Imprecise | All anatomical structures | |||||||||||||||||||||||||||
| 69382 | 69382 | SRR18516706 | SRX14647797 | SRS12413215 | SRP366491 | PRJNA821088 | Enhancer calling in early stages | PRJNA821088 | Other | CAGE seq for enhancer calling in early stages for the DANIO CODE project. | DCD007477BS | strain:WT|age:42hpf|dev stage:High pec|sex:not applicable|tissue:early embryonic cell|biomaterial provider:Mueller lab University of Birmingham|replicate:1|BioSampleModel:Model organism or animal | CAGE seq High pec Nuclear CAGE | DCD003737SQ | DCD003737SQ | max read length:50 | RNA-Seq | TRANSCRIPTOMIC | CAGE | SINGLE | ILLUMINA | Illumina HiSeq 2500 | SRP366491 | CAGE-seq_Mueller_lab_0014AS.DCD003737SQ.USERdanio-user.R1.fastq.gz | fastq | 1041108672.0 | 21689764.0 | CAGE seq Mueller lab 0014AS.DCD003737SQ.USERdanio user.R1.fastq.gz | 0:48 1:0 | A:233996885;C:276232291;G:275987771;T:254800016;N:91709 | 48 | 0 | 233996885 | 276232291 | 275987771 | 254800016 | 91709 | SRX14647797 | SRS12413215 | SRA1393740 | DANIO-CODE|Department for Biosciences and Nutrition | DANIO-CODE DANIO-CODE | 1 | 0.19665 | 0.0423 | 0.84271 | 0.49525 | 48 | B | usable mapping rate | illumina | hiseq_era | unknown | cage | unknown | bulk | unknown | unknown | Unknown | 2022-03-29 | Pharyngula | Embryo | Embryo Imprecise | All anatomical structures | |||||||||||||||||||||||||||
| 69383 | 69383 | SRR18516707 | SRX14647796 | SRS12413215 | SRP366491 | PRJNA821088 | Enhancer calling in early stages | PRJNA821088 | Other | CAGE seq for enhancer calling in early stages for the DANIO CODE project. | DCD007477BS | strain:WT|age:42hpf|dev stage:High pec|sex:not applicable|tissue:early embryonic cell|biomaterial provider:Mueller lab University of Birmingham|replicate:1|BioSampleModel:Model organism or animal | CAGE seq High pec Nuclear CAGE | DCD003729SQ | DCD003729SQ | max read length:50 | RNA-Seq | TRANSCRIPTOMIC | CAGE | SINGLE | ILLUMINA | Illumina HiSeq 2500 | SRP366491 | CAGE-seq_Mueller_lab_0014AS.DCD003729SQ.USERdanio-user.R1.fastq.gz | fastq | 383268528.0 | 7984761.0 | CAGE seq Mueller lab 0014AS.DCD003729SQ.USERdanio user.R1.fastq.gz | 0:48 1:0 | A:83789437;C:103371728;G:102876480;T:93198236;N:32647 | 48 | 0 | 83789437 | 103371728 | 102876480 | 93198236 | 32647 | SRX14647796 | SRS12413215 | SRA1393740 | DANIO-CODE|Department for Biosciences and Nutrition | DANIO-CODE DANIO-CODE | 1 | 0.15605 | 0.03059 | 0.86951 | 0.50369 | 48 | B | usable mapping rate | illumina | hiseq_era | unknown | cage | unknown | bulk | unknown | unknown | Unknown | 2022-03-29 | Pharyngula | Embryo | Embryo Imprecise | All anatomical structures | |||||||||||||||||||||||||||
| 69384 | 69384 | SRR18516708 | SRX14647795 | SRS12413215 | SRP366491 | PRJNA821088 | Enhancer calling in early stages | PRJNA821088 | Other | CAGE seq for enhancer calling in early stages for the DANIO CODE project. | DCD007477BS | strain:WT|age:42hpf|dev stage:High pec|sex:not applicable|tissue:early embryonic cell|biomaterial provider:Mueller lab University of Birmingham|replicate:1|BioSampleModel:Model organism or animal | CAGE seq High pec Nuclear CAGE | DCD003734SQ | DCD003734SQ | max read length:50 | RNA-Seq | TRANSCRIPTOMIC | CAGE | SINGLE | ILLUMINA | Illumina HiSeq 2500 | SRP366491 | CAGE-seq_Mueller_lab_0014AS.DCD003734SQ.USERdanio-user.R1.fastq.gz | fastq | 560665392.0 | 11680529.0 | CAGE seq Mueller lab 0014AS.DCD003734SQ.USERdanio user.R1.fastq.gz | 0:48 1:0 | A:123278501;C:149059346;G:152514412;T:135763704;N:49429 | 48 | 0 | 123278501 | 149059346 | 152514412 | 135763704 | 49429 | SRX14647795 | SRS12413215 | SRA1393740 | DANIO-CODE|Department for Biosciences and Nutrition | DANIO-CODE DANIO-CODE | 1 | 0.16518 | 0.02906 | 0.85283 | 0.47924 | 48 | B | usable mapping rate | illumina | hiseq_era | unknown | cage | unknown | bulk | unknown | unknown | Unknown | 2022-03-29 | Pharyngula | Embryo | Embryo Imprecise | All anatomical structures | |||||||||||||||||||||||||||
| 69385 | 69385 | SRR18516709 | SRX14647794 | SRS12413214 | SRP366491 | PRJNA821088 | Enhancer calling in early stages | PRJNA821088 | Other | CAGE seq for enhancer calling in early stages for the DANIO CODE project. | DCD007471BS | strain:WT|age:42hpf|dev stage:High pec|sex:not applicable|tissue:early embryonic cell|biomaterial provider:Mueller lab University of Birmingham|replicate:2|BioSampleModel:Model organism or animal | CAGE seq High pec Whole cell CAGE | DCD003731SQ | DCD003731SQ | max read length:50 | RNA-Seq | TRANSCRIPTOMIC | CAGE | SINGLE | ILLUMINA | Illumina HiSeq 2500 | SRP366491 | CAGE-seq_Mueller_lab_0013AS.DCD003731SQ.USERdanio-user.R1.fastq.gz | fastq | 534745104.0 | 11140523.0 | CAGE seq Mueller lab 0013AS.DCD003731SQ.USERdanio user.R1.fastq.gz | 0:48 1:0 | A:122900964;C:145291239;G:142314928;T:124199394;N:38579 | 48 | 0 | 122900964 | 145291239 | 142314928 | 124199394 | 38579 | SRX14647794 | SRS12413214 | SRA1393740 | DANIO-CODE|Department for Biosciences and Nutrition | DANIO-CODE DANIO-CODE | 1 | 0.21193 | 0.03662 | 0.8576 | 0.51061 | 48 | B | usable mapping rate | illumina | hiseq_era | unknown | cage | unknown | bulk | unknown | unknown | Unknown | 2022-03-29 | Pharyngula | Embryo | Embryo Imprecise | All anatomical structures | |||||||||||||||||||||||||||
| 69386 | 69386 | SRR18516710 | SRX14647793 | SRS12413214 | SRP366491 | PRJNA821088 | Enhancer calling in early stages | PRJNA821088 | Other | CAGE seq for enhancer calling in early stages for the DANIO CODE project. | DCD007471BS | strain:WT|age:42hpf|dev stage:High pec|sex:not applicable|tissue:early embryonic cell|biomaterial provider:Mueller lab University of Birmingham|replicate:2|BioSampleModel:Model organism or animal | CAGE seq High pec Whole cell CAGE | DCD003733SQ | DCD003733SQ | max read length:50 | RNA-Seq | TRANSCRIPTOMIC | CAGE | SINGLE | ILLUMINA | Illumina HiSeq 2500 | SRP366491 | CAGE-seq_Mueller_lab_0013AS.DCD003733SQ.USERdanio-user.R1.fastq.gz | fastq | 1106623152.0 | 23054649.0 | CAGE seq Mueller lab 0013AS.DCD003733SQ.USERdanio user.R1.fastq.gz | 0:48 1:0 | A:244437769;C:302018709;G:293780395;T:266329857;N:56422 | 48 | 0 | 244437769 | 302018709 | 293780395 | 266329857 | 56422 | SRX14647793 | SRS12413214 | SRA1393740 | DANIO-CODE|Department for Biosciences and Nutrition | DANIO-CODE DANIO-CODE | 1 | 0.25973 | 0.02959 | 0.84778 | 0.50948 | 48 | B | usable mapping rate | illumina | hiseq_era | unknown | cage | unknown | bulk | unknown | unknown | Unknown | 2022-03-29 | Pharyngula | Embryo | Embryo Imprecise | All anatomical structures | |||||||||||||||||||||||||||
| 69387 | 69387 | SRR18516711 | SRX14647792 | SRS12413214 | SRP366491 | PRJNA821088 | Enhancer calling in early stages | PRJNA821088 | Other | CAGE seq for enhancer calling in early stages for the DANIO CODE project. | DCD007471BS | strain:WT|age:42hpf|dev stage:High pec|sex:not applicable|tissue:early embryonic cell|biomaterial provider:Mueller lab University of Birmingham|replicate:2|BioSampleModel:Model organism or animal | CAGE seq High pec Whole cell CAGE | DCD003735SQ | DCD003735SQ | max read length:50 | RNA-Seq | TRANSCRIPTOMIC | CAGE | SINGLE | ILLUMINA | Illumina HiSeq 2500 | SRP366491 | CAGE-seq_Mueller_lab_0013AS.DCD003735SQ.USERdanio-user.R1.fastq.gz | fastq | 829987920.0 | 17291415.0 | CAGE seq Mueller lab 0013AS.DCD003735SQ.USERdanio user.R1.fastq.gz | 0:48 1:0 | A:189638839;C:226118233;G:218991435;T:195178858;N:60555 | 48 | 0 | 189638839 | 226118233 | 218991435 | 195178858 | 60555 | SRX14647792 | SRS12413214 | SRA1393740 | DANIO-CODE|Department for Biosciences and Nutrition | DANIO-CODE DANIO-CODE | 1 | 0.20953 | 0.03562 | 0.85285 | 0.52927 | 48 | B | usable mapping rate | illumina | hiseq_era | unknown | cage | unknown | bulk | unknown | unknown | Unknown | 2022-03-29 | Pharyngula | Embryo | Embryo Imprecise | All anatomical structures | |||||||||||||||||||||||||||
| 69388 | 69388 | SRR18516712 | SRX14647791 | SRS12413213 | SRP366491 | PRJNA821088 | Enhancer calling in early stages | PRJNA821088 | Other | CAGE seq for enhancer calling in early stages for the DANIO CODE project. | DCD007472BS | strain:WT|age:14hpf|dev stage:5 9 somites|sex:not applicable|tissue:early embryonic cell|biomaterial provider:Mueller lab University of Birmingham|replicate:2|BioSampleModel:Model organism or animal | CAGE seq 5 9 somites Whole cell CAGE | DCD003730SQ | DCD003730SQ | max read length:50 | RNA-Seq | TRANSCRIPTOMIC | CAGE | SINGLE | ILLUMINA | Illumina HiSeq 2500 | SRP366491 | CAGE-seq_Mueller_lab_0013AS.DCD003730SQ.USERdanio-user.R1.fastq.gz | fastq | 358730736.0 | 7473557.0 | CAGE seq Mueller lab 0013AS.DCD003730SQ.USERdanio user.R1.fastq.gz | 0:48 1:0 | A:82114871;C:96481695;G:97889447;T:82217691;N:27032 | 48 | 0 | 82114871 | 96481695 | 97889447 | 82217691 | 27032 | SRX14647791 | SRS12413213 | SRA1393740 | DANIO-CODE|Department for Biosciences and Nutrition | DANIO-CODE DANIO-CODE | 1 | 0.22955 | 0.03488 | 0.84719 | 0.51204 | 48 | B | usable mapping rate | illumina | hiseq_era | unknown | cage | unknown | bulk | unknown | unknown | Unknown | 2022-03-29 | Segmentation | Embryo | Embryo Imprecise | All anatomical structures | |||||||||||||||||||||||||||
| 69389 | 69389 | SRR18516713 | SRX14647790 | SRS12413209 | SRP366491 | PRJNA821088 | Enhancer calling in early stages | PRJNA821088 | Other | CAGE seq for enhancer calling in early stages for the DANIO CODE project. | DCD007478BS | strain:WT|age:4.7hpf|dev stage:zfs:0000015|sex:not applicable|tissue:early embryonic cell|biomaterial provider:Mueller lab University of Birmingham|replicate:1|BioSampleModel:Model organism or animal | CAGE seq zfs:0000015 Nuclear CAGE | DCD003732SQ | DCD003732SQ | max read length:50 | RNA-Seq | TRANSCRIPTOMIC | CAGE | SINGLE | ILLUMINA | Illumina HiSeq 2500 | SRP366491 | CAGE-seq_Mueller_lab_0014AS.DCD003732SQ.USERdanio-user.R1.fastq.gz | fastq | 833831760.0 | 17371495.0 | CAGE seq Mueller lab 0014AS.DCD003732SQ.USERdanio user.R1.fastq.gz | 0:48 1:0 | A:184529953;C:221676233;G:220734592;T:206820467;N:70515 | 48 | 0 | 184529953 | 221676233 | 220734592 | 206820467 | 70515 | SRX14647790 | SRS12413209 | SRA1393740 | DANIO-CODE|Department for Biosciences and Nutrition | DANIO-CODE DANIO-CODE | 1 | 0.16336 | 0.03655 | 0.88633 | 0.47894 | 48 | B | usable mapping rate | illumina | hiseq_era | unknown | cage | unknown | bulk | unknown | unknown | Unknown | 2022-03-29 | Blastula | Embryo | Embryo Imprecise | All anatomical structures | |||||||||||||||||||||||||||
| 69390 | 69390 | SRR18516714 | SRX14647789 | SRS12413213 | SRP366491 | PRJNA821088 | Enhancer calling in early stages | PRJNA821088 | Other | CAGE seq for enhancer calling in early stages for the DANIO CODE project. | DCD007472BS | strain:WT|age:14hpf|dev stage:5 9 somites|sex:not applicable|tissue:early embryonic cell|biomaterial provider:Mueller lab University of Birmingham|replicate:2|BioSampleModel:Model organism or animal | CAGE seq 5 9 somites Whole cell CAGE | DCD003738SQ | DCD003738SQ | max read length:50 | RNA-Seq | TRANSCRIPTOMIC | CAGE | SINGLE | ILLUMINA | Illumina HiSeq 2500 | SRP366491 | CAGE-seq_Mueller_lab_0013AS.DCD003738SQ.USERdanio-user.R1.fastq.gz | fastq | 1160622288.0 | 24179631.0 | CAGE seq Mueller lab 0013AS.DCD003738SQ.USERdanio user.R1.fastq.gz | 0:48 1:0 | A:261568282;C:313737609;G:310169539;T:275088632;N:58226 | 48 | 0 | 261568282 | 313737609 | 310169539 | 275088632 | 58226 | SRX14647789 | SRS12413213 | SRA1393740 | DANIO-CODE|Department for Biosciences and Nutrition | DANIO-CODE DANIO-CODE | 1 | 0.27025 | 0.0344 | 0.86505 | 0.50961 | 48 | B | usable mapping rate | illumina | hiseq_era | unknown | cage | unknown | bulk | unknown | unknown | Unknown | 2022-03-29 | Segmentation | Embryo | Embryo Imprecise | All anatomical structures | |||||||||||||||||||||||||||
| 69391 | 69391 | SRR18516715 | SRX14647788 | SRS12413213 | SRP366491 | PRJNA821088 | Enhancer calling in early stages | PRJNA821088 | Other | CAGE seq for enhancer calling in early stages for the DANIO CODE project. | DCD007472BS | strain:WT|age:14hpf|dev stage:5 9 somites|sex:not applicable|tissue:early embryonic cell|biomaterial provider:Mueller lab University of Birmingham|replicate:2|BioSampleModel:Model organism or animal | CAGE seq 5 9 somites Whole cell CAGE | DCD003722SQ | DCD003722SQ | max read length:50 | RNA-Seq | TRANSCRIPTOMIC | CAGE | SINGLE | ILLUMINA | Illumina HiSeq 2500 | SRP366491 | CAGE-seq_Mueller_lab_0013AS.DCD003722SQ.USERdanio-user.R1.fastq.gz | fastq | 666656256.0 | 13888672.0 | CAGE seq Mueller lab 0013AS.DCD003722SQ.USERdanio user.R1.fastq.gz | 0:48 1:0 | A:149770159;C:178068662;G:177661463;T:161122864;N:33108 | 48 | 0 | 149770159 | 178068662 | 177661463 | 161122864 | 33108 | SRX14647788 | SRS12413213 | SRA1393740 | DANIO-CODE|Department for Biosciences and Nutrition | DANIO-CODE DANIO-CODE | 1 | 0.25511 | 0.02671 | 0.86436 | 0.50531 | 48 | B | usable mapping rate | illumina | hiseq_era | unknown | cage | unknown | bulk | unknown | unknown | Unknown | 2022-03-29 | Segmentation | Embryo | Embryo Imprecise | All anatomical structures | |||||||||||||||||||||||||||
| 69392 | 69392 | SRR18516716 | SRX14647787 | SRS12413212 | SRP366491 | PRJNA821088 | Enhancer calling in early stages | PRJNA821088 | Other | CAGE seq for enhancer calling in early stages for the DANIO CODE project. | DCD007473BS | strain:WT|age:24hpf|dev stage:Prim 5|sex:not applicable|tissue:early embryonic cell|biomaterial provider:Mueller lab University of Birmingham|replicate:2|BioSampleModel:Model organism or animal | CAGE seq Prim Whole cell CAGE | DCD003727SQ | DCD003727SQ | max read length:50 | RNA-Seq | TRANSCRIPTOMIC | CAGE | SINGLE | ILLUMINA | Illumina HiSeq 2500 | SRP366491 | CAGE-seq_Mueller_lab_0013AS.DCD003727SQ.USERdanio-user.R1.fastq.gz | fastq | 924879888.0 | 19268331.0 | CAGE seq Mueller lab 0013AS.DCD003727SQ.USERdanio user.R1.fastq.gz | 0:48 1:0 | A:214005056;C:251321459;G:244648845;T:214835794;N:68734 | 48 | 0 | 214005056 | 251321459 | 244648845 | 214835794 | 68734 | SRX14647787 | SRS12413212 | SRA1393740 | DANIO-CODE|Department for Biosciences and Nutrition | DANIO-CODE DANIO-CODE | 1 | 0.2165 | 0.03701 | 0.84768 | 0.54102 | 48 | B | usable mapping rate | illumina | hiseq_era | unknown | cage | unknown | bulk | unknown | unknown | Unknown | 2022-03-29 | Pharyngula | Embryo | Embryo Imprecise | All anatomical structures | |||||||||||||||||||||||||||
| 69393 | 69393 | SRR18516717 | SRX14647786 | SRS12413212 | SRP366491 | PRJNA821088 | Enhancer calling in early stages | PRJNA821088 | Other | CAGE seq for enhancer calling in early stages for the DANIO CODE project. | DCD007473BS | strain:WT|age:24hpf|dev stage:Prim 5|sex:not applicable|tissue:early embryonic cell|biomaterial provider:Mueller lab University of Birmingham|replicate:2|BioSampleModel:Model organism or animal | CAGE seq Prim Whole cell CAGE | DCD003717SQ | DCD003717SQ | max read length:50 | RNA-Seq | TRANSCRIPTOMIC | CAGE | SINGLE | ILLUMINA | Illumina HiSeq 2500 | SRP366491 | CAGE-seq_Mueller_lab_0013AS.DCD003717SQ.USERdanio-user.R1.fastq.gz | fastq | 1485845376.0 | 30955112.0 | CAGE seq Mueller lab 0013AS.DCD003717SQ.USERdanio user.R1.fastq.gz | 0:48 1:0 | A:329122121;C:402514173;G:390388645;T:363745867;N:74570 | 48 | 0 | 329122121 | 402514173 | 390388645 | 363745867 | 74570 | SRX14647786 | SRS12413212 | SRA1393740 | DANIO-CODE|Department for Biosciences and Nutrition | DANIO-CODE DANIO-CODE | 1 | 0.23893 | 0.0248 | 0.86125 | 0.50849 | 48 | B | usable mapping rate | illumina | hiseq_era | unknown | cage | unknown | bulk | unknown | unknown | Unknown | 2022-03-29 | Pharyngula | Embryo | Embryo Imprecise | All anatomical structures | |||||||||||||||||||||||||||
| 69394 | 69394 | SRR18516718 | SRX14647785 | SRS12413212 | SRP366491 | PRJNA821088 | Enhancer calling in early stages | PRJNA821088 | Other | CAGE seq for enhancer calling in early stages for the DANIO CODE project. | DCD007473BS | strain:WT|age:24hpf|dev stage:Prim 5|sex:not applicable|tissue:early embryonic cell|biomaterial provider:Mueller lab University of Birmingham|replicate:2|BioSampleModel:Model organism or animal | CAGE seq Prim Whole cell CAGE | DCD003739SQ | DCD003739SQ | max read length:50 | RNA-Seq | TRANSCRIPTOMIC | CAGE | SINGLE | ILLUMINA | Illumina HiSeq 2500 | SRP366491 | CAGE-seq_Mueller_lab_0013AS.DCD003739SQ.USERdanio-user.R1.fastq.gz | fastq | 1372622736.0 | 28596307.0 | CAGE seq Mueller lab 0013AS.DCD003739SQ.USERdanio user.R1.fastq.gz | 0:48 1:0 | A:306709302;C:373224967;G:360990874;T:331626639;N:70954 | 48 | 0 | 306709302 | 373224967 | 360990874 | 331626639 | 70954 | SRX14647785 | SRS12413212 | SRA1393740 | DANIO-CODE|Department for Biosciences and Nutrition | DANIO-CODE DANIO-CODE | 1 | 0.26574 | 0.02914 | 0.85113 | 0.52147 | 48 | B | usable mapping rate | illumina | hiseq_era | unknown | cage | unknown | bulk | unknown | unknown | Unknown | 2022-03-29 | Pharyngula | Embryo | Embryo Imprecise | All anatomical structures | |||||||||||||||||||||||||||
| 69395 | 69395 | SRR18516719 | SRX14647784 | SRS12413211 | SRP366491 | PRJNA821088 | Enhancer calling in early stages | PRJNA821088 | Other | CAGE seq for enhancer calling in early stages for the DANIO CODE project. | DCD007474BS | strain:WT|age:14hpf|dev stage:5 9 somites|sex:not applicable|tissue:early embryonic cell|biomaterial provider:Mueller lab University of Birmingham|replicate:1|BioSampleModel:Model organism or animal | CAGE seq 5 9 somites Nuclear CAGE | DCD003721SQ | DCD003721SQ | max read length:50 | RNA-Seq | TRANSCRIPTOMIC | CAGE | SINGLE | ILLUMINA | Illumina HiSeq 2500 | SRP366491 | CAGE-seq_Mueller_lab_0014AS.DCD003721SQ.USERdanio-user.R1.fastq.gz | fastq | 425483904.0 | 8864248.0 | CAGE seq Mueller lab 0014AS.DCD003721SQ.USERdanio user.R1.fastq.gz | 0:48 1:0 | A:98642418;C:114264552;G:114112965;T:98433833;N:30136 | 48 | 0 | 98642418 | 114264552 | 114112965 | 98433833 | 30136 | SRX14647784 | SRS12413211 | SRA1393740 | DANIO-CODE|Department for Biosciences and Nutrition | DANIO-CODE DANIO-CODE | 1 | 0.21658 | 0.03797 | 0.85226 | 0.48604 | 48 | B | usable mapping rate | illumina | hiseq_era | unknown | cage | unknown | bulk | unknown | unknown | Unknown | 2022-03-29 | Segmentation | Embryo | Embryo Imprecise | All anatomical structures | |||||||||||||||||||||||||||
| 69396 | 69396 | SRR18516720 | SRX14647783 | SRS12413211 | SRP366491 | PRJNA821088 | Enhancer calling in early stages | PRJNA821088 | Other | CAGE seq for enhancer calling in early stages for the DANIO CODE project. | DCD007474BS | strain:WT|age:14hpf|dev stage:5 9 somites|sex:not applicable|tissue:early embryonic cell|biomaterial provider:Mueller lab University of Birmingham|replicate:1|BioSampleModel:Model organism or animal | CAGE seq 5 9 somites Nuclear CAGE | DCD003736SQ | DCD003736SQ | max read length:50 | RNA-Seq | TRANSCRIPTOMIC | CAGE | SINGLE | ILLUMINA | Illumina HiSeq 2500 | SRP366491 | CAGE-seq_Mueller_lab_0014AS.DCD003736SQ.USERdanio-user.R1.fastq.gz | fastq | 533681088.0 | 11118356.0 | CAGE seq Mueller lab 0014AS.DCD003736SQ.USERdanio user.R1.fastq.gz | 0:48 1:0 | A:115850766;C:142773032;G:143794343;T:131220159;N:42788 | 48 | 0 | 115850766 | 142773032 | 143794343 | 131220159 | 42788 | SRX14647783 | SRS12413211 | SRA1393740 | DANIO-CODE|Department for Biosciences and Nutrition | DANIO-CODE DANIO-CODE | 1 | 0.15054 | 0.0279 | 0.87434 | 0.4867 | 48 | B | usable mapping rate | illumina | hiseq_era | unknown | cage | unknown | bulk | unknown | unknown | Unknown | 2022-03-29 | Segmentation | Embryo | Embryo Imprecise | All anatomical structures | |||||||||||||||||||||||||||
| 69397 | 69397 | SRR18516721 | SRX14647782 | SRS12413211 | SRP366491 | PRJNA821088 | Enhancer calling in early stages | PRJNA821088 | Other | CAGE seq for enhancer calling in early stages for the DANIO CODE project. | DCD007474BS | strain:WT|age:14hpf|dev stage:5 9 somites|sex:not applicable|tissue:early embryonic cell|biomaterial provider:Mueller lab University of Birmingham|replicate:1|BioSampleModel:Model organism or animal | CAGE seq 5 9 somites Nuclear CAGE | DCD003726SQ | DCD003726SQ | max read length:50 | RNA-Seq | TRANSCRIPTOMIC | CAGE | SINGLE | ILLUMINA | Illumina HiSeq 2500 | SRP366491 | CAGE-seq_Mueller_lab_0014AS.DCD003726SQ.USERdanio-user.R1.fastq.gz | fastq | 250307520.0 | 5214740.0 | CAGE seq Mueller lab 0014AS.DCD003726SQ.USERdanio user.R1.fastq.gz | 0:48 1:0 | A:55705158;C:65429159;G:68119770;T:61031856;N:21577 | 48 | 0 | 55705158 | 65429159 | 68119770 | 61031856 | 21577 | SRX14647782 | SRS12413211 | SRA1393740 | DANIO-CODE|Department for Biosciences and Nutrition | DANIO-CODE DANIO-CODE | 1 | 0.19864 | 0.04157 | 0.85279 | 0.48786 | 48 | B | usable mapping rate | illumina | hiseq_era | unknown | cage | unknown | bulk | unknown | unknown | Unknown | 2022-03-29 | Segmentation | Embryo | Embryo Imprecise | All anatomical structures | |||||||||||||||||||||||||||
| 69398 | 69398 | SRR18516722 | SRX14647781 | SRS12413210 | SRP366491 | PRJNA821088 | Enhancer calling in early stages | PRJNA821088 | Other | CAGE seq for enhancer calling in early stages for the DANIO CODE project. | DCD007475BS | strain:WT|age:4.7hpf|dev stage:zfs:0000015|sex:not applicable|tissue:early embryonic cell|biomaterial provider:Mueller lab University of Birmingham|replicate:2|BioSampleModel:Model organism or animal | CAGE seq zfs:0000015 Whole cell CAGE | DCD003720SQ | DCD003720SQ | max read length:50 | RNA-Seq | TRANSCRIPTOMIC | CAGE | SINGLE | ILLUMINA | Illumina HiSeq 2500 | SRP366491 | CAGE-seq_Mueller_lab_0013AS.DCD003720SQ.USERdanio-user.R1.fastq.gz | fastq | 536292432.0 | 11172759.0 | CAGE seq Mueller lab 0013AS.DCD003720SQ.USERdanio user.R1.fastq.gz | 0:48 1:0 | A:122756671;C:142850582;G:140364671;T:130293257;N:27251 | 48 | 0 | 122756671 | 142850582 | 140364671 | 130293257 | 27251 | SRX14647781 | SRS12413210 | SRA1393740 | DANIO-CODE|Department for Biosciences and Nutrition | DANIO-CODE DANIO-CODE | 1 | 0.23072 | 0.04674 | 0.89572 | 0.59187 | 48 | B | usable mapping rate | illumina | hiseq_era | unknown | cage | unknown | bulk | unknown | unknown | Unknown | 2022-03-29 | Blastula | Embryo | Embryo Imprecise | All anatomical structures | |||||||||||||||||||||||||||
| 69399 | 69399 | SRR18516723 | SRX14647780 | SRS12413210 | SRP366491 | PRJNA821088 | Enhancer calling in early stages | PRJNA821088 | Other | CAGE seq for enhancer calling in early stages for the DANIO CODE project. | DCD007475BS | strain:WT|age:4.7hpf|dev stage:zfs:0000015|sex:not applicable|tissue:early embryonic cell|biomaterial provider:Mueller lab University of Birmingham|replicate:2|BioSampleModel:Model organism or animal | CAGE seq zfs:0000015 Whole cell CAGE | DCD003725SQ | DCD003725SQ | max read length:50 | RNA-Seq | TRANSCRIPTOMIC | CAGE | SINGLE | ILLUMINA | Illumina HiSeq 2500 | SRP366491 | CAGE-seq_Mueller_lab_0013AS.DCD003725SQ.USERdanio-user.R1.fastq.gz | fastq | 1839979776.0 | 38332912.0 | CAGE seq Mueller lab 0013AS.DCD003725SQ.USERdanio user.R1.fastq.gz | 0:48 1:0 | A:422772310;C:484519947;G:479159876;T:453437875;N:89768 | 48 | 0 | 422772310 | 484519947 | 479159876 | 453437875 | 89768 | SRX14647780 | SRS12413210 | SRA1393740 | DANIO-CODE|Department for Biosciences and Nutrition | DANIO-CODE DANIO-CODE | 1 | 0.22646 | 0.03978 | 0.88574 | 0.556 | 48 | B | usable mapping rate | illumina | hiseq_era | unknown | cage | unknown | bulk | unknown | unknown | Unknown | 2022-03-29 | Blastula | Embryo | Embryo Imprecise | All anatomical structures | |||||||||||||||||||||||||||
| 69400 | 69400 | SRR18516724 | SRX14647779 | SRS12413209 | SRP366491 | PRJNA821088 | Enhancer calling in early stages | PRJNA821088 | Other | CAGE seq for enhancer calling in early stages for the DANIO CODE project. | DCD007478BS | strain:WT|age:4.7hpf|dev stage:zfs:0000015|sex:not applicable|tissue:early embryonic cell|biomaterial provider:Mueller lab University of Birmingham|replicate:1|BioSampleModel:Model organism or animal | CAGE seq zfs:0000015 Nuclear CAGE | DCD003728SQ | DCD003728SQ | max read length:50 | RNA-Seq | TRANSCRIPTOMIC | CAGE | SINGLE | ILLUMINA | Illumina HiSeq 2500 | SRP366491 | CAGE-seq_Mueller_lab_0014AS.DCD003728SQ.USERdanio-user.R1.fastq.gz | fastq | 778510272.0 | 16218964.0 | CAGE seq Mueller lab 0014AS.DCD003728SQ.USERdanio user.R1.fastq.gz | 0:48 1:0 | A:170505572;C:207531743;G:206655879;T:193751108;N:65970 | 48 | 0 | 170505572 | 207531743 | 206655879 | 193751108 | 65970 | SRX14647779 | SRS12413209 | SRA1393740 | DANIO-CODE|Department for Biosciences and Nutrition | DANIO-CODE DANIO-CODE | 1 | 0.1419 | 0.02783 | 0.8954 | 0.49693 | 48 | B | usable mapping rate | illumina | hiseq_era | unknown | cage | unknown | bulk | unknown | unknown | Unknown | 2022-03-29 | Blastula | Embryo | Embryo Imprecise | All anatomical structures | |||||||||||||||||||||||||||
| 69401 | 69401 | SRR18516725 | SRX14647778 | SRS12413209 | SRP366491 | PRJNA821088 | Enhancer calling in early stages | PRJNA821088 | Other | CAGE seq for enhancer calling in early stages for the DANIO CODE project. | DCD007478BS | strain:WT|age:4.7hpf|dev stage:zfs:0000015|sex:not applicable|tissue:early embryonic cell|biomaterial provider:Mueller lab University of Birmingham|replicate:1|BioSampleModel:Model organism or animal | CAGE seq zfs:0000015 Nuclear CAGE | DCD003724SQ | DCD003724SQ | max read length:50 | RNA-Seq | TRANSCRIPTOMIC | CAGE | SINGLE | ILLUMINA | Illumina HiSeq 2500 | SRP366491 | CAGE-seq_Mueller_lab_0014AS.DCD003724SQ.USERdanio-user.R1.fastq.gz | fastq | 641108496.0 | 13356427.0 | CAGE seq Mueller lab 0014AS.DCD003724SQ.USERdanio user.R1.fastq.gz | 0:48 1:0 | A:143927298;C:167543966;G:171107575;T:158473503;N:56154 | 48 | 0 | 143927298 | 167543966 | 171107575 | 158473503 | 56154 | SRX14647778 | SRS12413209 | SRA1393740 | DANIO-CODE|Department for Biosciences and Nutrition | DANIO-CODE DANIO-CODE | 1 | 0.20203 | 0.0454 | 0.87091 | 0.51364 | 48 | B | usable mapping rate | illumina | hiseq_era | unknown | cage | unknown | bulk | unknown | unknown | Unknown | 2022-03-29 | Blastula | Embryo | Embryo Imprecise | All anatomical structures |
Advanced export
JSON shape: default, array, newline-delimited
CREATE TABLE run_metadata("run.accession" VARCHAR, "experiment.accession" VARCHAR, "sample.accession" VARCHAR, "study.accession" VARCHAR, bioproject VARCHAR, "study.title" VARCHAR, "study.alias" VARCHAR, "study.type" VARCHAR, "study.abstract" VARCHAR, "study.attributes" VARCHAR, "study.PMIDs" VARCHAR, "sample.description" VARCHAR, "sample.title" VARCHAR, "sample.alias" VARCHAR, "sample.centername" VARCHAR, "sample.attributes" VARCHAR, "GEOsample.title" VARCHAR, "GEOsample.dataprocessing" VARCHAR, "GEOsample.source" VARCHAR, "GEOsample.treatmentprotocol" VARCHAR, "GEOsample.extractprotocol" VARCHAR, "GEOsample.growthprotocol" VARCHAR, "GEOsample.characteristics" VARCHAR, "GEOsample.accession" VARCHAR, "experiment.title" VARCHAR, "experiment.alias" VARCHAR, "experiment.library_name" VARCHAR, "experiment.design_description" VARCHAR, "experiment.library_construction_protocol" VARCHAR, "experiment.attributes" VARCHAR, "experiment.library_strategy" VARCHAR, "experiment.library_source" VARCHAR, "experiment.library_selection" VARCHAR, "experiment.library_layout" VARCHAR, "experiment.platform" VARCHAR, "experiment.instrument_model" VARCHAR, "experiment.spot_descriptor" VARCHAR, "experiment.study_ref" VARCHAR, "run.title" VARCHAR, "run.attributes" VARCHAR, "run.filename" VARCHAR, "run.semantic_name" VARCHAR, "run.total_bases" DOUBLE, "run.total_spots" DOUBLE, "run.alias" VARCHAR, "run.read_lengths" VARCHAR, "run.base_counts" VARCHAR, "run.r1_length" BIGINT, "run.r2_length" BIGINT, "run.r3_length" BIGINT, "run.r4_length" BIGINT, "run.Acount" BIGINT, "run.Ccount" BIGINT, "run.Gcount" BIGINT, "run.Tcount" BIGINT, "run.Ncount" BIGINT, "run.experiment" VARCHAR, "run.pool_member" VARCHAR, "submission.accession" VARCHAR, "submission.srasource" VARCHAR, "submission.bioprojectsource" VARCHAR, "seqdetective.n_mates" BIGINT, "seqdetective.mapping_rate.mate1" DOUBLE, "seqdetective.mapping_rate.mate2" DOUBLE, "seqdetective.nofeature_rate.mate1" DOUBLE, "seqdetective.nofeature_rate.mate2" DOUBLE, "seqdetective.sparsity.mate1" DOUBLE, "seqdetective.sparsity.mate2" DOUBLE, "seqdetective.pos_strand_rate.mate1" DOUBLE, "seqdetective.pos_strand_rate.mate2" DOUBLE, "seqdetective.readlen.mate1" BIGINT, "seqdetective.readlen.mate2" BIGINT, "seqdetective.judgement.mate1" VARCHAR, "seqdetective.judgement.mate2" VARCHAR, "seqdetective.judgement.reason" VARCHAR, platform_family VARCHAR, instrument_generation VARCHAR, read_bias VARCHAR, selection_class VARCHAR, prep_kit VARCHAR, sc_or_bulk VARCHAR, tech_class VARCHAR, technology VARCHAR, tech_variant VARCHAR, "submission.bioprojectsource.country" VARCHAR, earliest_date DATE, devstage_curation VARCHAR, devstage_curation_coarse VARCHAR, tissue_curation VARCHAR, tissue_curation_coarse VARCHAR);;
CREATE INDEX idx_run_bioproject ON run_metadata(bioproject);;
CREATE INDEX idx_run_run_accession ON run_metadata("run.accession");;