metadata: run_metadata: 880 rows where experiment.library_layout = "PAIRED", technology = "smartseq" and tissue_curation

880 rows where experiment.library_layout = "PAIRED", technology = "smartseq" and tissue_curation_coarse = "Hematopoietic System"

descending

Link	rowid ▼	run.accession	experiment.accession	sample.accession	study.accession	bioproject	study.title	study.alias	study.type	study.abstract	study.attributes	sample.description	sample.title	sample.alias	sample.centername	sample.attributes	experiment.title	experiment.alias	experiment.library_name	experiment.design_description	experiment.library_construction_protocol	experiment.attributes	experiment.library_strategy	experiment.library_source	experiment.library_selection	experiment.library_layout	experiment.platform	experiment.instrument_model	experiment.spot_descriptor	experiment.study_ref	run.title	run.attributes	run.filename	run.semantic_name	run.total_bases	run.total_spots	run.alias	run.read_lengths	run.base_counts	run.r1_length	run.r2_length	run.Acount	run.Ccount	run.Gcount	run.Tcount	run.Ncount	run.experiment	run.pool_member	submission.accession	submission.srasource	submission.bioprojectsource	seqdetective.n_mates	seqdetective.mapping_rate.mate1	seqdetective.mapping_rate.mate2	seqdetective.nofeature_rate.mate1	seqdetective.nofeature_rate.mate2	seqdetective.sparsity.mate1	seqdetective.sparsity.mate2	seqdetective.pos_strand_rate.mate1	seqdetective.pos_strand_rate.mate2	seqdetective.readlen.mate1	seqdetective.readlen.mate2	seqdetective.judgement.mate1	seqdetective.judgement.mate2	seqdetective.judgement.reason	platform_family	instrument_generation	read_bias	selection_class	prep_kit	sc_or_bulk	tech_class	technology	submission.bioprojectsource.country	earliest_date	devstage_curation	devstage_curation_coarse	tissue_curation	tissue_curation_coarse
4162	4162	ERR1620593	ERX1690958	ERS1343646	ERP015799	PRJEB14175	Single cell RNA sequencing of spleen derived LCK cells from adult Zebrafish	E-MTAB-4617	Transcriptome Analysis	Transcriptome data from individual lck:GFP expressing cells isolated from adult Zebrafish spleen. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T cells and NK like and a minor one of myeloid like cells. Single cell transcriptomes are matched with FACS index sorting data GFP forward and side light scatter and dead cell staining	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2016 05 26\|ArrayExpress:E MTAB 4617	Protocols: Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	LCK 9#95	SAMEA4432197	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics	ENA first public:2016 12 01\|ENA last update:2016 09 12\|External Id:SAMEA4432197\|INSDC center alias:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC center name:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC first public:2016 12 01T17:01:25Z\|INSDC last update:2016 09 12T14:14:55Z\|INSDC status:public\|PI fluorescence:17\|Submitter Id:E MTAB 46171473686057:LCK 9#95\|age:3\|broker name:ArrayExpress\|common name:zebrafish\|developmental stage:adult\|forward scatter:31714\|genotype:heterozygous Tglck:EGFP\|gfp fluorescence:1238\|individual:2\|organism part:spleen\|plate:9\|sample name:E MTAB 46171473686057:LCK 9#95\|side scatter:36\|single cell well quality:good quality\|well:H11\|well information:single cell	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	E MTAB 46171473686057:LCK 9#95 p	LCK 9#95 p	Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	Experimental Factor: spleen:organism part\|Experimental Factor: 31714:forward scatter\|Experimental Factor: 36:side scatter\|Experimental Factor: 1238:GFP fluorescence	RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	ERP015799	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2018 11 16	LCK_9_95_1.fq.gz LCK_9_95_2.fq.gz	fastq fastq	474750.0	1899.0	E MTAB 46171473686057:LCK 9 95	0:125 1:125	A:136505;C:125919;G:96114;T:115865;N:347	125	125	136505	125919	96114	115865	347	ERX1690958	ERS1343646	ERA701304	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	2	0.64665	0.58253	0.06713	0.17475	0.99697	0.99918	0.62883	0.60975	125	125	B	B	biological fallback assumption	illumina	hiseq_era	full_length	random_priming	unknown	sc	single_cell_plate	smartseq	Switzerland	2016-05-26	Adult	Adult	Spleen	Hematopoietic System
4163	4163	ERR1620592	ERX1690957	ERS1343645	ERP015799	PRJEB14175	Single cell RNA sequencing of spleen derived LCK cells from adult Zebrafish	E-MTAB-4617	Transcriptome Analysis	Transcriptome data from individual lck:GFP expressing cells isolated from adult Zebrafish spleen. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T cells and NK like and a minor one of myeloid like cells. Single cell transcriptomes are matched with FACS index sorting data GFP forward and side light scatter and dead cell staining	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2016 05 26\|ArrayExpress:E MTAB 4617	Protocols: Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	LCK 9#94	SAMEA4432196	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics	ENA first public:2016 12 01\|ENA last update:2016 09 12\|External Id:SAMEA4432196\|INSDC center alias:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC center name:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC first public:2016 12 01T17:01:25Z\|INSDC last update:2016 09 12T14:14:55Z\|INSDC status:public\|PI fluorescence:9\|Submitter Id:E MTAB 46171473686057:LCK 9#94\|age:3\|broker name:ArrayExpress\|common name:zebrafish\|developmental stage:adult\|forward scatter:25724\|genotype:heterozygous Tglck:EGFP\|gfp fluorescence:725\|individual:2\|organism part:spleen\|plate:9\|sample name:E MTAB 46171473686057:LCK 9#94\|side scatter:35\|single cell well quality:good quality\|well:H10\|well information:single cell	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	E MTAB 46171473686057:LCK 9#94 p	LCK 9#94 p	Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	Experimental Factor: spleen:organism part\|Experimental Factor: 25724:forward scatter\|Experimental Factor: 35:side scatter\|Experimental Factor: 725:GFP fluorescence	RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	ERP015799	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2018 11 16	LCK_9_94_1.fq.gz LCK_9_94_2.fq.gz	fastq fastq	532198750.0	2128795.0	E MTAB 46171473686057:LCK 9 94	0:125 1:125	A:157501334;C:111259905;G:101266460;T:161502762;N:668289	125	125	157501334	111259905	101266460	161502762	668289	ERX1690957	ERS1343645	ERA701304	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	2	0.75986	0.75328	0.2661	0.26681	0.98614	0.98658	0.57082	0.58891	125	125	B	B	biological fallback assumption	illumina	hiseq_era	full_length	random_priming	unknown	sc	single_cell_plate	smartseq	Switzerland	2016-05-26	Adult	Adult	Spleen	Hematopoietic System
4164	4164	ERR1620591	ERX1690956	ERS1343644	ERP015799	PRJEB14175	Single cell RNA sequencing of spleen derived LCK cells from adult Zebrafish	E-MTAB-4617	Transcriptome Analysis	Transcriptome data from individual lck:GFP expressing cells isolated from adult Zebrafish spleen. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T cells and NK like and a minor one of myeloid like cells. Single cell transcriptomes are matched with FACS index sorting data GFP forward and side light scatter and dead cell staining	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2016 05 26\|ArrayExpress:E MTAB 4617	Protocols: Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	LCK 9#93	SAMEA4432195	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics	ENA first public:2016 12 01\|ENA last update:2016 09 12\|External Id:SAMEA4432195\|INSDC center alias:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC center name:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC first public:2016 12 01T17:01:25Z\|INSDC last update:2016 09 12T14:14:55Z\|INSDC status:public\|PI fluorescence:8\|Submitter Id:E MTAB 46171473686057:LCK 9#93\|age:3\|broker name:ArrayExpress\|common name:zebrafish\|developmental stage:adult\|forward scatter:36702\|genotype:heterozygous Tglck:EGFP\|gfp fluorescence:1420\|individual:2\|organism part:spleen\|plate:9\|sample name:E MTAB 46171473686057:LCK 9#93\|side scatter:60\|single cell well quality:good quality\|well:H9\|well information:single cell	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	E MTAB 46171473686057:LCK 9#93 p	LCK 9#93 p	Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	Experimental Factor: spleen:organism part\|Experimental Factor: 36702:forward scatter\|Experimental Factor: 60:side scatter\|Experimental Factor: 1420:GFP fluorescence	RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	ERP015799	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2018 11 16	LCK_9_93_1.fq.gz LCK_9_93_2.fq.gz	fastq fastq	1015072000.0	4060288.0	E MTAB 46171473686057:LCK 9 93	0:125 1:125	A:314298041;C:203125138;G:177015908;T:319243223;N:1389690	125	125	314298041	203125138	177015908	319243223	1389690	ERX1690956	ERS1343644	ERA701304	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	2	0.80228	0.79924	0.32196	0.32224	0.98638	0.98652	0.50107	0.5056	125	125	B	B	biological fallback assumption	illumina	hiseq_era	full_length	random_priming	unknown	sc	single_cell_plate	smartseq	Switzerland	2016-05-26	Adult	Adult	Spleen	Hematopoietic System
4165	4165	ERR1620590	ERX1690955	ERS1343643	ERP015799	PRJEB14175	Single cell RNA sequencing of spleen derived LCK cells from adult Zebrafish	E-MTAB-4617	Transcriptome Analysis	Transcriptome data from individual lck:GFP expressing cells isolated from adult Zebrafish spleen. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T cells and NK like and a minor one of myeloid like cells. Single cell transcriptomes are matched with FACS index sorting data GFP forward and side light scatter and dead cell staining	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2016 05 26\|ArrayExpress:E MTAB 4617	Protocols: Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	LCK 9#92	SAMEA4432194	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics	ENA first public:2016 12 01\|ENA last update:2016 09 12\|External Id:SAMEA4432194\|INSDC center alias:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC center name:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC first public:2016 12 01T17:01:25Z\|INSDC last update:2016 09 12T14:14:55Z\|INSDC status:public\|PI fluorescence:10\|Submitter Id:E MTAB 46171473686057:LCK 9#92\|age:3\|broker name:ArrayExpress\|common name:zebrafish\|developmental stage:adult\|forward scatter:30554\|genotype:heterozygous Tglck:EGFP\|gfp fluorescence:582\|individual:2\|organism part:spleen\|plate:9\|sample name:E MTAB 46171473686057:LCK 9#92\|side scatter:42\|single cell well quality:good quality\|well:H8\|well information:single cell	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	E MTAB 46171473686057:LCK 9#92 p	LCK 9#92 p	Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	Experimental Factor: spleen:organism part\|Experimental Factor: 30554:forward scatter\|Experimental Factor: 42:side scatter\|Experimental Factor: 582:GFP fluorescence	RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	ERP015799	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2019 01 23	LCK_9_92_1.fq LCK_9_92_2.fq	fastq fastq	438701250.0	1754805.0	E MTAB 46171473686057:LCK 9 92	0:125 1:125	A:132899983;C:89606484;G:80513081;T:135111829;N:569873	125	125	132899983	89606484	80513081	135111829	569873	ERX1690955	ERS1343643	ERA701304	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	2	0.79916	0.7974	0.36491	0.36755	0.98547	0.98575	0.54153	0.52254	125	125	B	B	biological fallback assumption	illumina	hiseq_era	full_length	random_priming	unknown	sc	single_cell_plate	smartseq	Switzerland	2016-05-26	Adult	Adult	Spleen	Hematopoietic System
4166	4166	ERR1620589	ERX1690954	ERS1343642	ERP015799	PRJEB14175	Single cell RNA sequencing of spleen derived LCK cells from adult Zebrafish	E-MTAB-4617	Transcriptome Analysis	Transcriptome data from individual lck:GFP expressing cells isolated from adult Zebrafish spleen. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T cells and NK like and a minor one of myeloid like cells. Single cell transcriptomes are matched with FACS index sorting data GFP forward and side light scatter and dead cell staining	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2016 05 26\|ArrayExpress:E MTAB 4617	Protocols: Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	LCK 9#91	SAMEA4432193	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics	ENA first public:2016 12 01\|ENA last update:2016 09 12\|External Id:SAMEA4432193\|INSDC center alias:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC center name:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC first public:2016 12 01T17:01:25Z\|INSDC last update:2016 09 12T14:14:55Z\|INSDC status:public\|PI fluorescence:22\|Submitter Id:E MTAB 46171473686057:LCK 9#91\|age:3\|broker name:ArrayExpress\|common name:zebrafish\|developmental stage:adult\|forward scatter:43052\|genotype:heterozygous Tglck:EGFP\|gfp fluorescence:761\|individual:2\|organism part:spleen\|plate:9\|sample name:E MTAB 46171473686057:LCK 9#91\|side scatter:71\|single cell well quality:good quality\|well:H7\|well information:single cell	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	E MTAB 46171473686057:LCK 9#91 p	LCK 9#91 p	Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	Experimental Factor: spleen:organism part\|Experimental Factor: 43052:forward scatter\|Experimental Factor: 71:side scatter\|Experimental Factor: 761:GFP fluorescence	RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	ERP015799	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2018 11 16	LCK_9_91_1.fq.gz LCK_9_91_2.fq.gz	fastq fastq	19000.0	76.0	E MTAB 46171473686057:LCK 9 91	0:125 1:125	A:4469;C:6507;G:4937;T:3087;N:0	125	125	4469	6507	4937	3087	0	ERX1690954	ERS1343642	ERA701304	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	2	0.7353	0.5	0.0147	0.5	0.99953	1.0	0.34693		125	125	B	T	mate2 technical by mapping diff	illumina	hiseq_era	full_length	random_priming	unknown	sc	single_cell_plate	smartseq	Switzerland	2016-05-26	Adult	Adult	Spleen	Hematopoietic System
4167	4167	ERR1620588	ERX1690953	ERS1343641	ERP015799	PRJEB14175	Single cell RNA sequencing of spleen derived LCK cells from adult Zebrafish	E-MTAB-4617	Transcriptome Analysis	Transcriptome data from individual lck:GFP expressing cells isolated from adult Zebrafish spleen. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T cells and NK like and a minor one of myeloid like cells. Single cell transcriptomes are matched with FACS index sorting data GFP forward and side light scatter and dead cell staining	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2016 05 26\|ArrayExpress:E MTAB 4617	Protocols: Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	LCK 9#90	SAMEA4432192	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics	ENA first public:2016 12 01\|ENA last update:2016 09 12\|External Id:SAMEA4432192\|INSDC center alias:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC center name:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC first public:2016 12 01T17:01:25Z\|INSDC last update:2016 09 12T14:14:55Z\|INSDC status:public\|PI fluorescence:21\|Submitter Id:E MTAB 46171473686057:LCK 9#90\|age:3\|broker name:ArrayExpress\|common name:zebrafish\|developmental stage:adult\|forward scatter:39430\|genotype:heterozygous Tglck:EGFP\|gfp fluorescence:1108\|individual:2\|organism part:spleen\|plate:9\|sample name:E MTAB 46171473686057:LCK 9#90\|side scatter:44\|single cell well quality:good quality\|well:H6\|well information:single cell	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	E MTAB 46171473686057:LCK 9#90 p	LCK 9#90 p	Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	Experimental Factor: spleen:organism part\|Experimental Factor: 39430:forward scatter\|Experimental Factor: 44:side scatter\|Experimental Factor: 1108:GFP fluorescence	RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	ERP015799	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2018 11 16	LCK_9_90_1.fq.gz LCK_9_90_2.fq.gz	fastq fastq	220510750.0	882043.0	E MTAB 46171473686057:LCK 9 90	0:125 1:125	A:63250451;C:48173039;G:44278169;T:64541378;N:267713	125	125	63250451	48173039	44278169	64541378	267713	ERX1690953	ERS1343641	ERA701304	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	2	0.74324	0.73809	0.23917	0.24021	0.98636	0.98742	0.59527	0.61371	125	125	B	B	biological fallback assumption	illumina	hiseq_era	full_length	random_priming	unknown	sc	single_cell_plate	smartseq	Switzerland	2016-05-26	Adult	Adult	Spleen	Hematopoietic System
4168	4168	ERR1620587	ERX1690952	ERS1343640	ERP015799	PRJEB14175	Single cell RNA sequencing of spleen derived LCK cells from adult Zebrafish	E-MTAB-4617	Transcriptome Analysis	Transcriptome data from individual lck:GFP expressing cells isolated from adult Zebrafish spleen. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T cells and NK like and a minor one of myeloid like cells. Single cell transcriptomes are matched with FACS index sorting data GFP forward and side light scatter and dead cell staining	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2016 05 26\|ArrayExpress:E MTAB 4617	Protocols: Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	LCK 9#9	SAMEA4432191	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics	ENA first public:2016 12 01\|ENA last update:2016 09 12\|External Id:SAMEA4432191\|INSDC center alias:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC center name:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC first public:2016 12 01T17:01:25Z\|INSDC last update:2016 09 12T14:14:55Z\|INSDC status:public\|PI fluorescence:30\|Submitter Id:E MTAB 46171473686057:LCK 9#9\|age:3\|broker name:ArrayExpress\|common name:zebrafish\|developmental stage:adult\|forward scatter:40567\|genotype:heterozygous Tglck:EGFP\|gfp fluorescence:1083\|individual:2\|organism part:spleen\|plate:9\|sample name:E MTAB 46171473686057:LCK 9#9\|side scatter:48\|single cell well quality:good quality\|well:A9\|well information:single cell	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	E MTAB 46171473686057:LCK 9#9 p	LCK 9#9 p	Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	Experimental Factor: spleen:organism part\|Experimental Factor: 40567:forward scatter\|Experimental Factor: 48:side scatter\|Experimental Factor: 1083:GFP fluorescence	RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	ERP015799	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2019 01 23	LCK_9_9_1.fq LCK_9_9_2.fq	fastq fastq	350162000.0	1400648.0	E MTAB 46171473686057:LCK 9 9	0:125 1:125	A:99955822;C:77407427;G:70474543;T:101918554;N:405654	125	125	99955822	77407427	70474543	101918554	405654	ERX1690952	ERS1343640	ERA701304	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	2	0.75741	0.75069	0.24338	0.24525	0.98474	0.98522	0.55927	0.44507	125	125	B	B	biological fallback assumption	illumina	hiseq_era	full_length	random_priming	unknown	sc	single_cell_plate	smartseq	Switzerland	2016-05-26	Adult	Adult	Spleen	Hematopoietic System
4169	4169	ERR1620586	ERX1690951	ERS1343639	ERP015799	PRJEB14175	Single cell RNA sequencing of spleen derived LCK cells from adult Zebrafish	E-MTAB-4617	Transcriptome Analysis	Transcriptome data from individual lck:GFP expressing cells isolated from adult Zebrafish spleen. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T cells and NK like and a minor one of myeloid like cells. Single cell transcriptomes are matched with FACS index sorting data GFP forward and side light scatter and dead cell staining	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2016 05 26\|ArrayExpress:E MTAB 4617	Protocols: Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	LCK 9#89	SAMEA4432190	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics	ENA first public:2016 12 01\|ENA last update:2016 09 12\|External Id:SAMEA4432190\|INSDC center alias:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC center name:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC first public:2016 12 01T17:01:25Z\|INSDC last update:2016 09 12T14:14:55Z\|INSDC status:public\|PI fluorescence:13\|Submitter Id:E MTAB 46171473686057:LCK 9#89\|age:3\|broker name:ArrayExpress\|common name:zebrafish\|developmental stage:adult\|forward scatter:30767\|genotype:heterozygous Tglck:EGFP\|gfp fluorescence:898\|individual:2\|organism part:spleen\|plate:9\|sample name:E MTAB 46171473686057:LCK 9#89\|side scatter:74\|single cell well quality:good quality\|well:H5\|well information:single cell	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	E MTAB 46171473686057:LCK 9#89 p	LCK 9#89 p	Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	Experimental Factor: spleen:organism part\|Experimental Factor: 30767:forward scatter\|Experimental Factor: 74:side scatter\|Experimental Factor: 898:GFP fluorescence	RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	ERP015799	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2019 01 23	LCK_9_89_1.fq LCK_9_89_2.fq	fastq fastq	130313750.0	521255.0	E MTAB 46171473686057:LCK 9 89	0:125 1:125	A:36426425;C:29173883;G:27225328;T:37343854;N:144260	125	125	36426425	29173883	27225328	37343854	144260	ERX1690951	ERS1343639	ERA701304	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	2	0.75406	0.7501	0.23832	0.24	0.98488	0.98565	0.60407	0.61002	125	125	B	B	biological fallback assumption	illumina	hiseq_era	full_length	random_priming	unknown	sc	single_cell_plate	smartseq	Switzerland	2016-05-26	Adult	Adult	Spleen	Hematopoietic System
4170	4170	ERR1620585	ERX1690950	ERS1343638	ERP015799	PRJEB14175	Single cell RNA sequencing of spleen derived LCK cells from adult Zebrafish	E-MTAB-4617	Transcriptome Analysis	Transcriptome data from individual lck:GFP expressing cells isolated from adult Zebrafish spleen. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T cells and NK like and a minor one of myeloid like cells. Single cell transcriptomes are matched with FACS index sorting data GFP forward and side light scatter and dead cell staining	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2016 05 26\|ArrayExpress:E MTAB 4617	Protocols: Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	LCK 9#88	SAMEA4432189	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics	ENA first public:2016 12 01\|ENA last update:2016 09 12\|External Id:SAMEA4432189\|INSDC center alias:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC center name:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC first public:2016 12 01T17:01:25Z\|INSDC last update:2016 09 12T14:14:55Z\|INSDC status:public\|PI fluorescence:not available\|Submitter Id:E MTAB 46171473686057:LCK 9#88\|age:3\|broker name:ArrayExpress\|common name:zebrafish\|developmental stage:adult\|forward scatter:not available\|genotype:heterozygous Tglck:EGFP\|gfp fluorescence:not available\|individual:2\|organism part:spleen\|plate:9\|sample name:E MTAB 46171473686057:LCK 9#88\|side scatter:not available\|single cell well quality:good quality\|well:H4\|well information:single cell	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	E MTAB 46171473686057:LCK 9#88 p	LCK 9#88 p	Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	Experimental Factor: spleen:organism part\|Experimental Factor: not available:forward scatter\|Experimental Factor: not available:side scatter\|Experimental Factor: not available:GFP fluorescence	RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	ERP015799	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2019 01 23	LCK_9_88_1.fq LCK_9_88_2.fq	fastq fastq	157769000.0	631076.0	E MTAB 46171473686057:LCK 9 88	0:125 1:125	A:44320565;C:35472215;G:32345382;T:45452971;N:177867	125	125	44320565	35472215	32345382	45452971	177867	ERX1690950	ERS1343638	ERA701304	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	2	0.71628	0.71191	0.20717	0.20803	0.98792	0.98825	0.55812	0.55103	125	125	B	B	biological fallback assumption	illumina	hiseq_era	full_length	random_priming	unknown	sc	single_cell_plate	smartseq	Switzerland	2016-05-26	Adult	Adult	Spleen	Hematopoietic System
4171	4171	ERR1620584	ERX1690949	ERS1343637	ERP015799	PRJEB14175	Single cell RNA sequencing of spleen derived LCK cells from adult Zebrafish	E-MTAB-4617	Transcriptome Analysis	Transcriptome data from individual lck:GFP expressing cells isolated from adult Zebrafish spleen. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T cells and NK like and a minor one of myeloid like cells. Single cell transcriptomes are matched with FACS index sorting data GFP forward and side light scatter and dead cell staining	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2016 05 26\|ArrayExpress:E MTAB 4617	Protocols: Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	LCK 9#87	SAMEA4432188	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics	ENA first public:2016 12 01\|ENA last update:2016 09 12\|External Id:SAMEA4432188\|INSDC center alias:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC center name:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC first public:2016 12 01T17:01:25Z\|INSDC last update:2016 09 12T14:14:55Z\|INSDC status:public\|PI fluorescence:not available\|Submitter Id:E MTAB 46171473686057:LCK 9#87\|age:3\|broker name:ArrayExpress\|common name:zebrafish\|developmental stage:adult\|forward scatter:not available\|genotype:heterozygous Tglck:EGFP\|gfp fluorescence:not available\|individual:2\|organism part:spleen\|plate:9\|sample name:E MTAB 46171473686057:LCK 9#87\|side scatter:not available\|single cell well quality:good quality\|well:H3\|well information:single cell	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	E MTAB 46171473686057:LCK 9#87 p	LCK 9#87 p	Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	Experimental Factor: spleen:organism part\|Experimental Factor: not available:forward scatter\|Experimental Factor: not available:side scatter\|Experimental Factor: not available:GFP fluorescence	RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	ERP015799	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2018 11 16	LCK_9_87_1.fq.gz LCK_9_87_2.fq.gz	fastq fastq	197086000.0	788344.0	E MTAB 46171473686057:LCK 9 87	0:125 1:125	A:55271572;C:44060136;G:40899852;T:56618046;N:236394	125	125	55271572	44060136	40899852	56618046	236394	ERX1690949	ERS1343637	ERA701304	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	2	0.78208	0.78182	0.21141	0.21368	0.98202	0.98265	0.5754	0.57068	125	125	B	B	biological fallback assumption	illumina	hiseq_era	full_length	random_priming	unknown	sc	single_cell_plate	smartseq	Switzerland	2016-05-26	Adult	Adult	Spleen	Hematopoietic System
4172	4172	ERR1620583	ERX1690948	ERS1343636	ERP015799	PRJEB14175	Single cell RNA sequencing of spleen derived LCK cells from adult Zebrafish	E-MTAB-4617	Transcriptome Analysis	Transcriptome data from individual lck:GFP expressing cells isolated from adult Zebrafish spleen. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T cells and NK like and a minor one of myeloid like cells. Single cell transcriptomes are matched with FACS index sorting data GFP forward and side light scatter and dead cell staining	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2016 05 26\|ArrayExpress:E MTAB 4617	Protocols: Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	LCK 9#86	SAMEA4432187	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics	ENA first public:2016 12 01\|ENA last update:2016 09 12\|External Id:SAMEA4432187\|INSDC center alias:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC center name:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC first public:2016 12 01T17:01:25Z\|INSDC last update:2016 09 12T14:14:55Z\|INSDC status:public\|PI fluorescence:not available\|Submitter Id:E MTAB 46171473686057:LCK 9#86\|age:3\|broker name:ArrayExpress\|common name:zebrafish\|developmental stage:adult\|forward scatter:not available\|genotype:heterozygous Tglck:EGFP\|gfp fluorescence:not available\|individual:2\|organism part:spleen\|plate:9\|sample name:E MTAB 46171473686057:LCK 9#86\|side scatter:not available\|single cell well quality:good quality\|well:H2\|well information:single cell	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	E MTAB 46171473686057:LCK 9#86 p	LCK 9#86 p	Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	Experimental Factor: spleen:organism part\|Experimental Factor: not available:forward scatter\|Experimental Factor: not available:side scatter\|Experimental Factor: not available:GFP fluorescence	RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	ERP015799	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2018 11 16	LCK_9_86_1.fq.gz LCK_9_86_2.fq.gz	fastq fastq	224629000.0	898516.0	E MTAB 46171473686057:LCK 9 86	0:125 1:125	A:61770403;C:51450915;G:47900404;T:63262980;N:244298	125	125	61770403	51450915	47900404	63262980	244298	ERX1690948	ERS1343636	ERA701304	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	2	0.76028	0.75975	0.19225	0.19404	0.98668	0.98689	0.61927	0.61793	125	125	B	B	biological fallback assumption	illumina	hiseq_era	full_length	random_priming	unknown	sc	single_cell_plate	smartseq	Switzerland	2016-05-26	Adult	Adult	Spleen	Hematopoietic System
4173	4173	ERR1620582	ERX1690947	ERS1343635	ERP015799	PRJEB14175	Single cell RNA sequencing of spleen derived LCK cells from adult Zebrafish	E-MTAB-4617	Transcriptome Analysis	Transcriptome data from individual lck:GFP expressing cells isolated from adult Zebrafish spleen. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T cells and NK like and a minor one of myeloid like cells. Single cell transcriptomes are matched with FACS index sorting data GFP forward and side light scatter and dead cell staining	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2016 05 26\|ArrayExpress:E MTAB 4617	Protocols: Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	LCK 9#85	SAMEA4432186	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics	ENA first public:2016 12 01\|ENA last update:2016 09 12\|External Id:SAMEA4432186\|INSDC center alias:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC center name:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC first public:2016 12 01T17:01:25Z\|INSDC last update:2016 09 12T14:14:55Z\|INSDC status:public\|PI fluorescence:not available\|Submitter Id:E MTAB 46171473686057:LCK 9#85\|age:3\|broker name:ArrayExpress\|common name:zebrafish\|developmental stage:adult\|forward scatter:not available\|genotype:heterozygous Tglck:EGFP\|gfp fluorescence:not available\|individual:2\|organism part:spleen\|plate:9\|sample name:E MTAB 46171473686057:LCK 9#85\|side scatter:not available\|single cell well quality:good quality\|well:H1\|well information:single cell	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	E MTAB 46171473686057:LCK 9#85 p	LCK 9#85 p	Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	Experimental Factor: spleen:organism part\|Experimental Factor: not available:forward scatter\|Experimental Factor: not available:side scatter\|Experimental Factor: not available:GFP fluorescence	RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	ERP015799	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2018 11 16	LCK_9_85_1.fq.gz LCK_9_85_2.fq.gz	fastq fastq	199000500.0	796002.0	E MTAB 46171473686057:LCK 9 85	0:125 1:125	A:55193275;C:45423281;G:41496742;T:56668394;N:218808	125	125	55193275	45423281	41496742	56668394	218808	ERX1690947	ERS1343635	ERA701304	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	2	0.74084	0.73899	0.20224	0.20349	0.9867	0.98687	0.59679	0.59056	125	125	B	B	biological fallback assumption	illumina	hiseq_era	full_length	random_priming	unknown	sc	single_cell_plate	smartseq	Switzerland	2016-05-26	Adult	Adult	Spleen	Hematopoietic System
4174	4174	ERR1620581	ERX1690946	ERS1343634	ERP015799	PRJEB14175	Single cell RNA sequencing of spleen derived LCK cells from adult Zebrafish	E-MTAB-4617	Transcriptome Analysis	Transcriptome data from individual lck:GFP expressing cells isolated from adult Zebrafish spleen. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T cells and NK like and a minor one of myeloid like cells. Single cell transcriptomes are matched with FACS index sorting data GFP forward and side light scatter and dead cell staining	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2016 05 26\|ArrayExpress:E MTAB 4617	Protocols: Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	LCK 9#84	SAMEA4432185	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics	ENA first public:2016 12 01\|ENA last update:2016 09 12\|External Id:SAMEA4432185\|INSDC center alias:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC center name:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC first public:2016 12 01T17:01:25Z\|INSDC last update:2016 09 12T14:14:55Z\|INSDC status:public\|PI fluorescence:18\|Submitter Id:E MTAB 46171473686057:LCK 9#84\|age:3\|broker name:ArrayExpress\|common name:zebrafish\|developmental stage:adult\|forward scatter:35715\|genotype:heterozygous Tglck:EGFP\|gfp fluorescence:873\|individual:2\|organism part:spleen\|plate:9\|sample name:E MTAB 46171473686057:LCK 9#84\|side scatter:36\|single cell well quality:good quality\|well:G12\|well information:single cell	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	E MTAB 46171473686057:LCK 9#84 p	LCK 9#84 p	Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	Experimental Factor: spleen:organism part\|Experimental Factor: 35715:forward scatter\|Experimental Factor: 36:side scatter\|Experimental Factor: 873:GFP fluorescence	RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	ERP015799	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2018 11 16	LCK_9_84_1.fq.gz LCK_9_84_2.fq.gz	fastq fastq	28062500.0	112250.0	E MTAB 46171473686057:LCK 9 84	0:125 1:125	A:7032031;C:7142576;G:6553430;T:7301171;N:33292	125	125	7032031	7142576	6553430	7301171	33292	ERX1690946	ERS1343634	ERA701304	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	2	0.00187	0.00012	0.00013	7e-05	0.99862	0.99993	0.63432	0.66666	125	125	T	T	mates < 9% mapping rate	illumina	hiseq_era	full_length	random_priming	unknown	sc	single_cell_plate	smartseq	Switzerland	2016-05-26	Adult	Adult	Spleen	Hematopoietic System
4175	4175	ERR1620580	ERX1690945	ERS1343633	ERP015799	PRJEB14175	Single cell RNA sequencing of spleen derived LCK cells from adult Zebrafish	E-MTAB-4617	Transcriptome Analysis	Transcriptome data from individual lck:GFP expressing cells isolated from adult Zebrafish spleen. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T cells and NK like and a minor one of myeloid like cells. Single cell transcriptomes are matched with FACS index sorting data GFP forward and side light scatter and dead cell staining	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2016 05 26\|ArrayExpress:E MTAB 4617	Protocols: Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	LCK 9#83	SAMEA4432184	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics	ENA first public:2016 12 01\|ENA last update:2016 09 12\|External Id:SAMEA4432184\|INSDC center alias:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC center name:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC first public:2016 12 01T17:01:25Z\|INSDC last update:2016 09 12T14:14:55Z\|INSDC status:public\|PI fluorescence:37\|Submitter Id:E MTAB 46171473686057:LCK 9#83\|age:3\|broker name:ArrayExpress\|common name:zebrafish\|developmental stage:adult\|forward scatter:40728\|genotype:heterozygous Tglck:EGFP\|gfp fluorescence:938\|individual:2\|organism part:spleen\|plate:9\|sample name:E MTAB 46171473686057:LCK 9#83\|side scatter:44\|single cell well quality:good quality\|well:G11\|well information:single cell	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	E MTAB 46171473686057:LCK 9#83 p	LCK 9#83 p	Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	Experimental Factor: spleen:organism part\|Experimental Factor: 40728:forward scatter\|Experimental Factor: 44:side scatter\|Experimental Factor: 938:GFP fluorescence	RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	ERP015799	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2018 11 16	LCK_9_83_1.fq.gz LCK_9_83_2.fq.gz	fastq fastq	52189500.0	208758.0	E MTAB 46171473686057:LCK 9 83	0:125 1:125	A:14138371;C:12080023;G:11427877;T:14490075;N:53154	125	125	14138371	12080023	11427877	14490075	53154	ERX1690945	ERS1343633	ERA701304	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	2	0.69436	0.6858	0.1889	0.18939	0.97973	0.98269	0.55341	0.54981	125	125	B	B	biological fallback assumption	illumina	hiseq_era	full_length	random_priming	unknown	sc	single_cell_plate	smartseq	Switzerland	2016-05-26	Adult	Adult	Spleen	Hematopoietic System
4176	4176	ERR1620579	ERX1690944	ERS1343632	ERP015799	PRJEB14175	Single cell RNA sequencing of spleen derived LCK cells from adult Zebrafish	E-MTAB-4617	Transcriptome Analysis	Transcriptome data from individual lck:GFP expressing cells isolated from adult Zebrafish spleen. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T cells and NK like and a minor one of myeloid like cells. Single cell transcriptomes are matched with FACS index sorting data GFP forward and side light scatter and dead cell staining	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2016 05 26\|ArrayExpress:E MTAB 4617	Protocols: Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	LCK 9#82	SAMEA4432183	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics	ENA first public:2016 12 01\|ENA last update:2016 09 12\|External Id:SAMEA4432183\|INSDC center alias:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC center name:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC first public:2016 12 01T17:01:25Z\|INSDC last update:2016 09 12T14:14:55Z\|INSDC status:public\|PI fluorescence:25\|Submitter Id:E MTAB 46171473686057:LCK 9#82\|age:3\|broker name:ArrayExpress\|common name:zebrafish\|developmental stage:adult\|forward scatter:33459\|genotype:heterozygous Tglck:EGFP\|gfp fluorescence:1082\|individual:2\|organism part:spleen\|plate:9\|sample name:E MTAB 46171473686057:LCK 9#82\|side scatter:48\|single cell well quality:good quality\|well:G10\|well information:single cell	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	E MTAB 46171473686057:LCK 9#82 p	LCK 9#82 p	Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	Experimental Factor: spleen:organism part\|Experimental Factor: 33459:forward scatter\|Experimental Factor: 48:side scatter\|Experimental Factor: 1082:GFP fluorescence	RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	ERP015799	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2018 11 16	LCK_9_82_1.fq LCK_9_82_2.fq	fastq fastq	58284250.0	233137.0	E MTAB 46171473686057:LCK 9 82	0:125 1:125	A:15859573;C:13476821;G:12691537;T:16188954;N:67365	125	125	15859573	13476821	12691537	16188954	67365	ERX1690944	ERS1343632	ERA701304	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	2	0.75001	0.74552	0.18627	0.18864	0.9822	0.98648	0.60937	0.60999	125	125	B	B	biological fallback assumption	illumina	hiseq_era	full_length	random_priming	unknown	sc	single_cell_plate	smartseq	Switzerland	2016-05-26	Adult	Adult	Spleen	Hematopoietic System
4177	4177	ERR1620578	ERX1690943	ERS1343631	ERP015799	PRJEB14175	Single cell RNA sequencing of spleen derived LCK cells from adult Zebrafish	E-MTAB-4617	Transcriptome Analysis	Transcriptome data from individual lck:GFP expressing cells isolated from adult Zebrafish spleen. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T cells and NK like and a minor one of myeloid like cells. Single cell transcriptomes are matched with FACS index sorting data GFP forward and side light scatter and dead cell staining	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2016 05 26\|ArrayExpress:E MTAB 4617	Protocols: Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	LCK 9#81	SAMEA4432182	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics	ENA first public:2016 12 01\|ENA last update:2016 09 12\|External Id:SAMEA4432182\|INSDC center alias:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC center name:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC first public:2016 12 01T17:01:25Z\|INSDC last update:2016 09 12T14:14:55Z\|INSDC status:public\|PI fluorescence:33\|Submitter Id:E MTAB 46171473686057:LCK 9#81\|age:3\|broker name:ArrayExpress\|common name:zebrafish\|developmental stage:adult\|forward scatter:31707\|genotype:heterozygous Tglck:EGFP\|gfp fluorescence:810\|individual:2\|organism part:spleen\|plate:9\|sample name:E MTAB 46171473686057:LCK 9#81\|side scatter:38\|single cell well quality:good quality\|well:G9\|well information:single cell	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	E MTAB 46171473686057:LCK 9#81 p	LCK 9#81 p	Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	Experimental Factor: spleen:organism part\|Experimental Factor: 31707:forward scatter\|Experimental Factor: 38:side scatter\|Experimental Factor: 810:GFP fluorescence	RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	ERP015799	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2018 11 16	LCK_9_81_1.fq LCK_9_81_2.fq	fastq fastq	68489250.0	273957.0	E MTAB 46171473686057:LCK 9 81	0:125 1:125	A:19370861;C:15351988;G:14127103;T:19568110;N:71188	125	125	19370861	15351988	14127103	19568110	71188	ERX1690943	ERS1343631	ERA701304	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	2	0.69077	0.68342	0.23054	0.2327	0.98309	0.98662	0.52377	0.52621	125	125	B	B	biological fallback assumption	illumina	hiseq_era	full_length	random_priming	unknown	sc	single_cell_plate	smartseq	Switzerland	2016-05-26	Adult	Adult	Spleen	Hematopoietic System
4178	4178	ERR1620577	ERX1690942	ERS1343630	ERP015799	PRJEB14175	Single cell RNA sequencing of spleen derived LCK cells from adult Zebrafish	E-MTAB-4617	Transcriptome Analysis	Transcriptome data from individual lck:GFP expressing cells isolated from adult Zebrafish spleen. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T cells and NK like and a minor one of myeloid like cells. Single cell transcriptomes are matched with FACS index sorting data GFP forward and side light scatter and dead cell staining	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2016 05 26\|ArrayExpress:E MTAB 4617	Protocols: Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	LCK 9#80	SAMEA4432181	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics	ENA first public:2016 12 01\|ENA last update:2016 09 12\|External Id:SAMEA4432181\|INSDC center alias:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC center name:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC first public:2016 12 01T17:01:25Z\|INSDC last update:2016 09 12T14:14:55Z\|INSDC status:public\|PI fluorescence:67\|Submitter Id:E MTAB 46171473686057:LCK 9#80\|age:3\|broker name:ArrayExpress\|common name:zebrafish\|developmental stage:adult\|forward scatter:29352\|genotype:heterozygous Tglck:EGFP\|gfp fluorescence:830\|individual:2\|organism part:spleen\|plate:9\|sample name:E MTAB 46171473686057:LCK 9#80\|side scatter:31\|single cell well quality:good quality\|well:G8\|well information:single cell	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	E MTAB 46171473686057:LCK 9#80 p	LCK 9#80 p	Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	Experimental Factor: spleen:organism part\|Experimental Factor: 29352:forward scatter\|Experimental Factor: 31:side scatter\|Experimental Factor: 830:GFP fluorescence	RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	ERP015799	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2018 11 16	LCK_9_80_1.fq LCK_9_80_2.fq	fastq fastq	86685500.0	346742.0	E MTAB 46171473686057:LCK 9 80	0:125 1:125	A:23598476;C:20045959;G:18812137;T:24137061;N:91867	125	125	23598476	20045959	18812137	24137061	91867	ERX1690942	ERS1343630	ERA701304	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	2	0.55748	0.55152	0.19719	0.19946	0.98634	0.98839	0.59414	0.599	125	125	B	B	biological fallback assumption	illumina	hiseq_era	full_length	random_priming	unknown	sc	single_cell_plate	smartseq	Switzerland	2016-05-26	Adult	Adult	Spleen	Hematopoietic System
4179	4179	ERR1620576	ERX1690941	ERS1343629	ERP015799	PRJEB14175	Single cell RNA sequencing of spleen derived LCK cells from adult Zebrafish	E-MTAB-4617	Transcriptome Analysis	Transcriptome data from individual lck:GFP expressing cells isolated from adult Zebrafish spleen. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T cells and NK like and a minor one of myeloid like cells. Single cell transcriptomes are matched with FACS index sorting data GFP forward and side light scatter and dead cell staining	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2016 05 26\|ArrayExpress:E MTAB 4617	Protocols: Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	LCK 9#8	SAMEA4432180	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics	ENA first public:2016 12 01\|ENA last update:2016 09 12\|External Id:SAMEA4432180\|INSDC center alias:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC center name:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC first public:2016 12 01T17:01:25Z\|INSDC last update:2016 09 12T14:14:55Z\|INSDC status:public\|PI fluorescence:19\|Submitter Id:E MTAB 46171473686057:LCK 9#8\|age:3\|broker name:ArrayExpress\|common name:zebrafish\|developmental stage:adult\|forward scatter:35145\|genotype:heterozygous Tglck:EGFP\|gfp fluorescence:1250\|individual:2\|organism part:spleen\|plate:9\|sample name:E MTAB 46171473686057:LCK 9#8\|side scatter:42\|single cell well quality:good quality\|well:A8\|well information:single cell	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	E MTAB 46171473686057:LCK 9#8 p	LCK 9#8 p	Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	Experimental Factor: spleen:organism part\|Experimental Factor: 35145:forward scatter\|Experimental Factor: 42:side scatter\|Experimental Factor: 1250:GFP fluorescence	RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	ERP015799	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2019 01 23	LCK_9_8_1.fq LCK_9_8_2.fq	fastq fastq	238347000.0	953388.0	E MTAB 46171473686057:LCK 9 8	0:125 1:125	A:65782361;C:54488168;G:50601970;T:67189207;N:285294	125	125	65782361	54488168	50601970	67189207	285294	ERX1690941	ERS1343629	ERA701304	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	2	0.65518	0.64464	0.18978	0.18905	0.98693	0.98748	0.52754	0.56709	125	125	B	B	biological fallback assumption	illumina	hiseq_era	full_length	random_priming	unknown	sc	single_cell_plate	smartseq	Switzerland	2016-05-26	Adult	Adult	Spleen	Hematopoietic System
4180	4180	ERR1620575	ERX1690940	ERS1343628	ERP015799	PRJEB14175	Single cell RNA sequencing of spleen derived LCK cells from adult Zebrafish	E-MTAB-4617	Transcriptome Analysis	Transcriptome data from individual lck:GFP expressing cells isolated from adult Zebrafish spleen. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T cells and NK like and a minor one of myeloid like cells. Single cell transcriptomes are matched with FACS index sorting data GFP forward and side light scatter and dead cell staining	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2016 05 26\|ArrayExpress:E MTAB 4617	Protocols: Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	LCK 9#79	SAMEA4432179	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics	ENA first public:2016 12 01\|ENA last update:2016 09 12\|External Id:SAMEA4432179\|INSDC center alias:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC center name:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC first public:2016 12 01T17:01:25Z\|INSDC last update:2016 09 12T14:14:55Z\|INSDC status:public\|PI fluorescence:20\|Submitter Id:E MTAB 46171473686057:LCK 9#79\|age:3\|broker name:ArrayExpress\|common name:zebrafish\|developmental stage:adult\|forward scatter:41645\|genotype:heterozygous Tglck:EGFP\|gfp fluorescence:1623\|individual:2\|organism part:spleen\|plate:9\|sample name:E MTAB 46171473686057:LCK 9#79\|side scatter:61\|single cell well quality:good quality\|well:G7\|well information:single cell	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	E MTAB 46171473686057:LCK 9#79 p	LCK 9#79 p	Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	Experimental Factor: spleen:organism part\|Experimental Factor: 41645:forward scatter\|Experimental Factor: 61:side scatter\|Experimental Factor: 1623:GFP fluorescence	RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	ERP015799	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2018 11 16	LCK_9_79_1.fq.gz LCK_9_79_2.fq.gz	fastq fastq	23250.0	93.0	E MTAB 46171473686057:LCK 9 79	0:125 1:125	A:5549;C:8313;G:5681;T:3703;N:4	125	125	5549	8313	5681	3703	4	ERX1690940	ERS1343628	ERA701304	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	2	0.76389	0.0	0.04166	0.0	0.99937	1.0	0.63461		125	125	B	T	mate2 technical by mapping diff	illumina	hiseq_era	full_length	random_priming	unknown	sc	single_cell_plate	smartseq	Switzerland	2016-05-26	Adult	Adult	Spleen	Hematopoietic System
4181	4181	ERR1620574	ERX1690939	ERS1343627	ERP015799	PRJEB14175	Single cell RNA sequencing of spleen derived LCK cells from adult Zebrafish	E-MTAB-4617	Transcriptome Analysis	Transcriptome data from individual lck:GFP expressing cells isolated from adult Zebrafish spleen. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T cells and NK like and a minor one of myeloid like cells. Single cell transcriptomes are matched with FACS index sorting data GFP forward and side light scatter and dead cell staining	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2016 05 26\|ArrayExpress:E MTAB 4617	Protocols: Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	LCK 9#78	SAMEA4432178	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics	ENA first public:2016 12 01\|ENA last update:2016 09 12\|External Id:SAMEA4432178\|INSDC center alias:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC center name:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC first public:2016 12 01T17:01:25Z\|INSDC last update:2016 09 12T14:14:55Z\|INSDC status:public\|PI fluorescence:18\|Submitter Id:E MTAB 46171473686057:LCK 9#78\|age:3\|broker name:ArrayExpress\|common name:zebrafish\|developmental stage:adult\|forward scatter:31337\|genotype:heterozygous Tglck:EGFP\|gfp fluorescence:585\|individual:2\|organism part:spleen\|plate:9\|sample name:E MTAB 46171473686057:LCK 9#78\|side scatter:31\|single cell well quality:good quality\|well:G6\|well information:single cell	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	E MTAB 46171473686057:LCK 9#78 p	LCK 9#78 p	Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	Experimental Factor: spleen:organism part\|Experimental Factor: 31337:forward scatter\|Experimental Factor: 31:side scatter\|Experimental Factor: 585:GFP fluorescence	RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	ERP015799	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2018 11 16	LCK_9_78_1.fq LCK_9_78_2.fq	fastq fastq	68756500.0	275026.0	E MTAB 46171473686057:LCK 9 78	0:125 1:125	A:18812651;C:15763743;G:14907361;T:19199426;N:73319	125	125	18812651	15763743	14907361	19199426	73319	ERX1690939	ERS1343627	ERA701304	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	2	0.76332	0.76198	0.15922	0.16204	0.9808	0.9833	0.47909	0.55176	125	125	B	B	biological fallback assumption	illumina	hiseq_era	full_length	random_priming	unknown	sc	single_cell_plate	smartseq	Switzerland	2016-05-26	Adult	Adult	Spleen	Hematopoietic System
4182	4182	ERR1620573	ERX1690938	ERS1343626	ERP015799	PRJEB14175	Single cell RNA sequencing of spleen derived LCK cells from adult Zebrafish	E-MTAB-4617	Transcriptome Analysis	Transcriptome data from individual lck:GFP expressing cells isolated from adult Zebrafish spleen. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T cells and NK like and a minor one of myeloid like cells. Single cell transcriptomes are matched with FACS index sorting data GFP forward and side light scatter and dead cell staining	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2016 05 26\|ArrayExpress:E MTAB 4617	Protocols: Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	LCK 9#77	SAMEA4432177	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics	ENA first public:2016 12 01\|ENA last update:2016 09 12\|External Id:SAMEA4432177\|INSDC center alias:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC center name:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC first public:2016 12 01T17:01:25Z\|INSDC last update:2016 09 12T14:14:55Z\|INSDC status:public\|PI fluorescence:20\|Submitter Id:E MTAB 46171473686057:LCK 9#77\|age:3\|broker name:ArrayExpress\|common name:zebrafish\|developmental stage:adult\|forward scatter:30123\|genotype:heterozygous Tglck:EGFP\|gfp fluorescence:603\|individual:2\|organism part:spleen\|plate:9\|sample name:E MTAB 46171473686057:LCK 9#77\|side scatter:50\|single cell well quality:good quality\|well:G5\|well information:single cell	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	E MTAB 46171473686057:LCK 9#77 p	LCK 9#77 p	Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	Experimental Factor: spleen:organism part\|Experimental Factor: 30123:forward scatter\|Experimental Factor: 50:side scatter\|Experimental Factor: 603:GFP fluorescence	RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	ERP015799	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2018 11 16	LCK_9_77_1.fq.gz LCK_9_77_2.fq.gz	fastq fastq	42395500.0	169582.0	E MTAB 46171473686057:LCK 9 77	0:125 1:125	A:11103947;C:10179905;G:9662627;T:11403946;N:45075	125	125	11103947	10179905	9662627	11403946	45075	ERX1690938	ERS1343626	ERA701304	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	2	0.38171	0.38131	0.16861	0.1712	0.98293	0.98498	0.57433	0.58312	125	125	B	B	biological fallback assumption	illumina	hiseq_era	full_length	random_priming	unknown	sc	single_cell_plate	smartseq	Switzerland	2016-05-26	Adult	Adult	Spleen	Hematopoietic System
4183	4183	ERR1620572	ERX1690937	ERS1343625	ERP015799	PRJEB14175	Single cell RNA sequencing of spleen derived LCK cells from adult Zebrafish	E-MTAB-4617	Transcriptome Analysis	Transcriptome data from individual lck:GFP expressing cells isolated from adult Zebrafish spleen. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T cells and NK like and a minor one of myeloid like cells. Single cell transcriptomes are matched with FACS index sorting data GFP forward and side light scatter and dead cell staining	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2016 05 26\|ArrayExpress:E MTAB 4617	Protocols: Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	LCK 9#73	SAMEA4432176	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics	ENA first public:2016 12 01\|ENA last update:2016 09 12\|External Id:SAMEA4432176\|INSDC center alias:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC center name:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC first public:2016 12 01T17:01:25Z\|INSDC last update:2016 09 12T14:14:55Z\|INSDC status:public\|PI fluorescence:27\|Submitter Id:E MTAB 46171473686057:LCK 9#73\|age:3\|broker name:ArrayExpress\|common name:zebrafish\|developmental stage:adult\|forward scatter:26913\|genotype:heterozygous Tglck:EGFP\|gfp fluorescence:986\|individual:2\|organism part:spleen\|plate:9\|sample name:E MTAB 46171473686057:LCK 9#73\|side scatter:32\|single cell well quality:good quality\|well:G1\|well information:single cell	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	E MTAB 46171473686057:LCK 9#73 p	LCK 9#73 p	Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	Experimental Factor: spleen:organism part\|Experimental Factor: 26913:forward scatter\|Experimental Factor: 32:side scatter\|Experimental Factor: 986:GFP fluorescence	RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	ERP015799	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2018 11 16	LCK_9_73_1.fq LCK_9_73_2.fq	fastq fastq	37170250.0	148681.0	E MTAB 46171473686057:LCK 9 73	0:125 1:125	A:9388197;C:9542010;G:8403521;T:9795513;N:41009	125	125	9388197	9542010	8403521	9795513	41009	ERX1690937	ERS1343625	ERA701304	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	2	0.00216	0.00026	0.00031	0.00014	0.99793	0.99977	0.6	0.33333	125	125	T	T	mates < 9% mapping rate	illumina	hiseq_era	full_length	random_priming	unknown	sc	single_cell_plate	smartseq	Switzerland	2016-05-26	Adult	Adult	Spleen	Hematopoietic System
4184	4184	ERR1620571	ERX1690936	ERS1343624	ERP015799	PRJEB14175	Single cell RNA sequencing of spleen derived LCK cells from adult Zebrafish	E-MTAB-4617	Transcriptome Analysis	Transcriptome data from individual lck:GFP expressing cells isolated from adult Zebrafish spleen. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T cells and NK like and a minor one of myeloid like cells. Single cell transcriptomes are matched with FACS index sorting data GFP forward and side light scatter and dead cell staining	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2016 05 26\|ArrayExpress:E MTAB 4617	Protocols: Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	LCK 9#72	SAMEA4432175	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics	ENA first public:2016 12 01\|ENA last update:2016 09 12\|External Id:SAMEA4432175\|INSDC center alias:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC center name:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC first public:2016 12 01T17:01:25Z\|INSDC last update:2016 09 12T14:14:55Z\|INSDC status:public\|PI fluorescence:28\|Submitter Id:E MTAB 46171473686057:LCK 9#72\|age:3\|broker name:ArrayExpress\|common name:zebrafish\|developmental stage:adult\|forward scatter:34915\|genotype:heterozygous Tglck:EGFP\|gfp fluorescence:806\|individual:2\|organism part:spleen\|plate:9\|sample name:E MTAB 46171473686057:LCK 9#72\|side scatter:28\|single cell well quality:good quality\|well:F12\|well information:single cell	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	E MTAB 46171473686057:LCK 9#72 p	LCK 9#72 p	Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	Experimental Factor: spleen:organism part\|Experimental Factor: 34915:forward scatter\|Experimental Factor: 28:side scatter\|Experimental Factor: 806:GFP fluorescence	RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	ERP015799	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2019 01 23	LCK_9_72_1.fq LCK_9_72_2.fq	fastq fastq	253547500.0	1014190.0	E MTAB 46171473686057:LCK 9 72	0:125 1:125	A:68815104;C:58428601;G:55787415;T:70317269;N:199111	125	125	68815104	58428601	55787415	70317269	199111	ERX1690936	ERS1343624	ERA701304	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	2	0.53639	0.51058	0.20937	0.19956	0.98877	0.9891	0.60475	0.58998	125	125	B	B	biological fallback assumption	illumina	hiseq_era	full_length	random_priming	unknown	sc	single_cell_plate	smartseq	Switzerland	2016-05-26	Adult	Adult	Spleen	Hematopoietic System
4185	4185	ERR1620570	ERX1690935	ERS1343623	ERP015799	PRJEB14175	Single cell RNA sequencing of spleen derived LCK cells from adult Zebrafish	E-MTAB-4617	Transcriptome Analysis	Transcriptome data from individual lck:GFP expressing cells isolated from adult Zebrafish spleen. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T cells and NK like and a minor one of myeloid like cells. Single cell transcriptomes are matched with FACS index sorting data GFP forward and side light scatter and dead cell staining	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2016 05 26\|ArrayExpress:E MTAB 4617	Protocols: Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	LCK 9#71	SAMEA4432174	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics	ENA first public:2016 12 01\|ENA last update:2016 09 12\|External Id:SAMEA4432174\|INSDC center alias:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC center name:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC first public:2016 12 01T17:01:25Z\|INSDC last update:2016 09 12T14:14:55Z\|INSDC status:public\|PI fluorescence:14\|Submitter Id:E MTAB 46171473686057:LCK 9#71\|age:3\|broker name:ArrayExpress\|common name:zebrafish\|developmental stage:adult\|forward scatter:37554\|genotype:heterozygous Tglck:EGFP\|gfp fluorescence:2246\|individual:2\|organism part:spleen\|plate:9\|sample name:E MTAB 46171473686057:LCK 9#71\|side scatter:46\|single cell well quality:good quality\|well:F11\|well information:single cell	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	E MTAB 46171473686057:LCK 9#71 p	LCK 9#71 p	Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	Experimental Factor: spleen:organism part\|Experimental Factor: 37554:forward scatter\|Experimental Factor: 46:side scatter\|Experimental Factor: 2246:GFP fluorescence	RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	ERP015799	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2018 11 16	LCK_9_71_1.fq LCK_9_71_2.fq	fastq fastq	920935750.0	3683743.0	E MTAB 46171473686057:LCK 9 71	0:125 1:125	A:279433924;C:182320675;G:173554894;T:284750509;N:875748	125	125	279433924	182320675	173554894	284750509	875748	ERX1690935	ERS1343623	ERA701304	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	2	0.83063	0.82446	0.35999	0.35776	0.98263	0.98291	0.56573	0.54032	125	125	B	B	biological fallback assumption	illumina	hiseq_era	full_length	random_priming	unknown	sc	single_cell_plate	smartseq	Switzerland	2016-05-26	Adult	Adult	Spleen	Hematopoietic System
4186	4186	ERR1620569	ERX1690934	ERS1343622	ERP015799	PRJEB14175	Single cell RNA sequencing of spleen derived LCK cells from adult Zebrafish	E-MTAB-4617	Transcriptome Analysis	Transcriptome data from individual lck:GFP expressing cells isolated from adult Zebrafish spleen. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T cells and NK like and a minor one of myeloid like cells. Single cell transcriptomes are matched with FACS index sorting data GFP forward and side light scatter and dead cell staining	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2016 05 26\|ArrayExpress:E MTAB 4617	Protocols: Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	LCK 9#70	SAMEA4432173	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics	ENA first public:2016 12 01\|ENA last update:2016 09 12\|External Id:SAMEA4432173\|INSDC center alias:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC center name:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC first public:2016 12 01T17:01:25Z\|INSDC last update:2016 09 12T14:14:55Z\|INSDC status:public\|PI fluorescence:8\|Submitter Id:E MTAB 46171473686057:LCK 9#70\|age:3\|broker name:ArrayExpress\|common name:zebrafish\|developmental stage:adult\|forward scatter:26499\|genotype:heterozygous Tglck:EGFP\|gfp fluorescence:277\|individual:2\|organism part:spleen\|plate:9\|sample name:E MTAB 46171473686057:LCK 9#70\|side scatter:22\|single cell well quality:good quality\|well:F10\|well information:single cell	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	E MTAB 46171473686057:LCK 9#70 p	LCK 9#70 p	Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	Experimental Factor: spleen:organism part\|Experimental Factor: 26499:forward scatter\|Experimental Factor: 22:side scatter\|Experimental Factor: 277:GFP fluorescence	RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	ERP015799	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2018 11 16	LCK_9_70_1.fq LCK_9_70_2.fq	fastq fastq	1789625250.0	7158501.0	E MTAB 46171473686057:LCK 9 70	0:125 1:125	A:543842907;C:353622858;G:335898082;T:554482225;N:1779178	125	125	543842907	353622858	335898082	554482225	1779178	ERX1690934	ERS1343622	ERA701304	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	2	0.81639	0.80979	0.41926	0.41697	0.98839	0.98841	0.60185	0.59731	125	125	B	B	biological fallback assumption	illumina	hiseq_era	full_length	random_priming	unknown	sc	single_cell_plate	smartseq	Switzerland	2016-05-26	Adult	Adult	Spleen	Hematopoietic System
4187	4187	ERR1620568	ERX1690933	ERS1343621	ERP015799	PRJEB14175	Single cell RNA sequencing of spleen derived LCK cells from adult Zebrafish	E-MTAB-4617	Transcriptome Analysis	Transcriptome data from individual lck:GFP expressing cells isolated from adult Zebrafish spleen. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T cells and NK like and a minor one of myeloid like cells. Single cell transcriptomes are matched with FACS index sorting data GFP forward and side light scatter and dead cell staining	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2016 05 26\|ArrayExpress:E MTAB 4617	Protocols: Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	LCK 9#7	SAMEA4432172	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics	ENA first public:2016 12 01\|ENA last update:2016 09 12\|External Id:SAMEA4432172\|INSDC center alias:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC center name:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC first public:2016 12 01T17:01:25Z\|INSDC last update:2016 09 12T14:14:55Z\|INSDC status:public\|PI fluorescence:37\|Submitter Id:E MTAB 46171473686057:LCK 9#7\|age:3\|broker name:ArrayExpress\|common name:zebrafish\|developmental stage:adult\|forward scatter:38988\|genotype:heterozygous Tglck:EGFP\|gfp fluorescence:709\|individual:2\|organism part:spleen\|plate:9\|sample name:E MTAB 46171473686057:LCK 9#7\|side scatter:64\|single cell well quality:good quality\|well:A7\|well information:single cell	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	E MTAB 46171473686057:LCK 9#7 p	LCK 9#7 p	Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	Experimental Factor: spleen:organism part\|Experimental Factor: 38988:forward scatter\|Experimental Factor: 64:side scatter\|Experimental Factor: 709:GFP fluorescence	RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	ERP015799	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2018 11 16	LCK_9_7_1.fq LCK_9_7_2.fq	fastq fastq	24250.0	97.0	E MTAB 46171473686057:LCK 9 7	0:125 1:125	A:5769;C:8295;G:6392;T:3794;N:0	125	125	5769	8295	6392	3794	0	ERX1690933	ERS1343621	ERA701304	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	2	0.56627	0.66667	0.02409	0.0	0.99953	0.99991	0.57142	0.25	125	125	B	B	biological fallback assumption	illumina	hiseq_era	full_length	random_priming	unknown	sc	single_cell_plate	smartseq	Switzerland	2016-05-26	Adult	Adult	Spleen	Hematopoietic System
4188	4188	ERR1620567	ERX1690932	ERS1343620	ERP015799	PRJEB14175	Single cell RNA sequencing of spleen derived LCK cells from adult Zebrafish	E-MTAB-4617	Transcriptome Analysis	Transcriptome data from individual lck:GFP expressing cells isolated from adult Zebrafish spleen. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T cells and NK like and a minor one of myeloid like cells. Single cell transcriptomes are matched with FACS index sorting data GFP forward and side light scatter and dead cell staining	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2016 05 26\|ArrayExpress:E MTAB 4617	Protocols: Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	LCK 9#69	SAMEA4432171	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics	ENA first public:2016 12 01\|ENA last update:2016 09 12\|External Id:SAMEA4432171\|INSDC center alias:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC center name:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC first public:2016 12 01T17:01:25Z\|INSDC last update:2016 09 12T14:14:55Z\|INSDC status:public\|PI fluorescence:38\|Submitter Id:E MTAB 46171473686057:LCK 9#69\|age:3\|broker name:ArrayExpress\|common name:zebrafish\|developmental stage:adult\|forward scatter:34705\|genotype:heterozygous Tglck:EGFP\|gfp fluorescence:1264\|individual:2\|organism part:spleen\|plate:9\|sample name:E MTAB 46171473686057:LCK 9#69\|side scatter:31\|single cell well quality:good quality\|well:F9\|well information:single cell	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	E MTAB 46171473686057:LCK 9#69 p	LCK 9#69 p	Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	Experimental Factor: spleen:organism part\|Experimental Factor: 34705:forward scatter\|Experimental Factor: 31:side scatter\|Experimental Factor: 1264:GFP fluorescence	RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	ERP015799	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2018 11 16	LCK_9_69_1.fq.gz LCK_9_69_2.fq.gz	fastq fastq	2813411000.0	11253644.0	E MTAB 46171473686057:LCK 9 69	0:125 1:125	A:849151900;C:573999347;G:527666010;T:859867519;N:2726224	125	125	849151900	573999347	527666010	859867519	2726224	ERX1690932	ERS1343620	ERA701304	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	2	0.77553	0.76235	0.32494	0.32029	0.98892	0.9893	0.50414	0.5235	125	125	B	B	biological fallback assumption	illumina	hiseq_era	full_length	random_priming	unknown	sc	single_cell_plate	smartseq	Switzerland	2016-05-26	Adult	Adult	Spleen	Hematopoietic System
4189	4189	ERR1620566	ERX1690931	ERS1343619	ERP015799	PRJEB14175	Single cell RNA sequencing of spleen derived LCK cells from adult Zebrafish	E-MTAB-4617	Transcriptome Analysis	Transcriptome data from individual lck:GFP expressing cells isolated from adult Zebrafish spleen. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T cells and NK like and a minor one of myeloid like cells. Single cell transcriptomes are matched with FACS index sorting data GFP forward and side light scatter and dead cell staining	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2016 05 26\|ArrayExpress:E MTAB 4617	Protocols: Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	LCK 9#68	SAMEA4432170	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics	ENA first public:2016 12 01\|ENA last update:2016 09 12\|External Id:SAMEA4432170\|INSDC center alias:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC center name:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC first public:2016 12 01T17:01:25Z\|INSDC last update:2016 09 12T14:14:55Z\|INSDC status:public\|PI fluorescence:28\|Submitter Id:E MTAB 46171473686057:LCK 9#68\|age:3\|broker name:ArrayExpress\|common name:zebrafish\|developmental stage:adult\|forward scatter:39308\|genotype:heterozygous Tglck:EGFP\|gfp fluorescence:1572\|individual:2\|organism part:spleen\|plate:9\|sample name:E MTAB 46171473686057:LCK 9#68\|side scatter:45\|single cell well quality:good quality\|well:F8\|well information:single cell	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	E MTAB 46171473686057:LCK 9#68 p	LCK 9#68 p	Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	Experimental Factor: spleen:organism part\|Experimental Factor: 39308:forward scatter\|Experimental Factor: 45:side scatter\|Experimental Factor: 1572:GFP fluorescence	RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	ERP015799	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2018 11 16	LCK_9_68_1.fq.gz LCK_9_68_2.fq.gz	fastq fastq	2216839000.0	8867356.0	E MTAB 46171473686057:LCK 9 68	0:125 1:125	A:668046554;C:444199358;G:424960844;T:677485270;N:2146974	125	125	668046554	444199358	424960844	677485270	2146974	ERX1690931	ERS1343619	ERA701304	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	2	0.89575	0.89254	0.38551	0.38478	0.98549	0.98567	0.71592	0.7066	125	125	B	B	biological fallback assumption	illumina	hiseq_era	full_length	random_priming	unknown	sc	single_cell_plate	smartseq	Switzerland	2016-05-26	Adult	Adult	Spleen	Hematopoietic System
4190	4190	ERR1620565	ERX1690930	ERS1343618	ERP015799	PRJEB14175	Single cell RNA sequencing of spleen derived LCK cells from adult Zebrafish	E-MTAB-4617	Transcriptome Analysis	Transcriptome data from individual lck:GFP expressing cells isolated from adult Zebrafish spleen. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T cells and NK like and a minor one of myeloid like cells. Single cell transcriptomes are matched with FACS index sorting data GFP forward and side light scatter and dead cell staining	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2016 05 26\|ArrayExpress:E MTAB 4617	Protocols: Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	LCK 9#67	SAMEA4432169	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics	ENA first public:2016 12 01\|ENA last update:2016 09 12\|External Id:SAMEA4432169\|INSDC center alias:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC center name:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC first public:2016 12 01T17:01:25Z\|INSDC last update:2016 09 12T14:14:55Z\|INSDC status:public\|PI fluorescence:20\|Submitter Id:E MTAB 46171473686057:LCK 9#67\|age:3\|broker name:ArrayExpress\|common name:zebrafish\|developmental stage:adult\|forward scatter:34235\|genotype:heterozygous Tglck:EGFP\|gfp fluorescence:349\|individual:2\|organism part:spleen\|plate:9\|sample name:E MTAB 46171473686057:LCK 9#67\|side scatter:32\|single cell well quality:good quality\|well:F7\|well information:single cell	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	E MTAB 46171473686057:LCK 9#67 p	LCK 9#67 p	Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	Experimental Factor: spleen:organism part\|Experimental Factor: 34235:forward scatter\|Experimental Factor: 32:side scatter\|Experimental Factor: 349:GFP fluorescence	RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	ERP015799	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2018 11 16	LCK_9_67_1.fq.gz LCK_9_67_2.fq.gz	fastq fastq	35750.0	143.0	E MTAB 46171473686057:LCK 9 67	0:125 1:125	A:8808;C:10966;G:8665;T:7301;N:10	125	125	8808	10966	8665	7301	10	ERX1690930	ERS1343618	ERA701304	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	2	0.69492	0.56	0.12711	0.28	0.99941	0.99989	0.64179	0.85714	125	125	B	B	biological fallback assumption	illumina	hiseq_era	full_length	random_priming	unknown	sc	single_cell_plate	smartseq	Switzerland	2016-05-26	Adult	Adult	Spleen	Hematopoietic System
4191	4191	ERR1620564	ERX1690929	ERS1343617	ERP015799	PRJEB14175	Single cell RNA sequencing of spleen derived LCK cells from adult Zebrafish	E-MTAB-4617	Transcriptome Analysis	Transcriptome data from individual lck:GFP expressing cells isolated from adult Zebrafish spleen. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T cells and NK like and a minor one of myeloid like cells. Single cell transcriptomes are matched with FACS index sorting data GFP forward and side light scatter and dead cell staining	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2016 05 26\|ArrayExpress:E MTAB 4617	Protocols: Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	LCK 9#66	SAMEA4432168	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics	ENA first public:2016 12 01\|ENA last update:2016 09 12\|External Id:SAMEA4432168\|INSDC center alias:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC center name:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC first public:2016 12 01T17:01:25Z\|INSDC last update:2016 09 12T14:14:55Z\|INSDC status:public\|PI fluorescence:18\|Submitter Id:E MTAB 46171473686057:LCK 9#66\|age:3\|broker name:ArrayExpress\|common name:zebrafish\|developmental stage:adult\|forward scatter:33415\|genotype:heterozygous Tglck:EGFP\|gfp fluorescence:681\|individual:2\|organism part:spleen\|plate:9\|sample name:E MTAB 46171473686057:LCK 9#66\|side scatter:38\|single cell well quality:good quality\|well:F6\|well information:single cell	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	E MTAB 46171473686057:LCK 9#66 p	LCK 9#66 p	Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	Experimental Factor: spleen:organism part\|Experimental Factor: 33415:forward scatter\|Experimental Factor: 38:side scatter\|Experimental Factor: 681:GFP fluorescence	RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	ERP015799	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2019 01 23	LCK_9_66_1.fq LCK_9_66_2.fq	fastq fastq	706985000.0	2827940.0	E MTAB 46171473686057:LCK 9 66	0:125 1:125	A:214112483;C:140731528;G:135079288;T:216394040;N:667661	125	125	214112483	140731528	135079288	216394040	667661	ERX1690929	ERS1343617	ERA701304	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	2	0.85974	0.85858	0.29344	0.29204	0.98695	0.98719	0.5047	0.58447	125	125	B	B	biological fallback assumption	illumina	hiseq_era	full_length	random_priming	unknown	sc	single_cell_plate	smartseq	Switzerland	2016-05-26	Adult	Adult	Spleen	Hematopoietic System
4192	4192	ERR1620563	ERX1690928	ERS1343616	ERP015799	PRJEB14175	Single cell RNA sequencing of spleen derived LCK cells from adult Zebrafish	E-MTAB-4617	Transcriptome Analysis	Transcriptome data from individual lck:GFP expressing cells isolated from adult Zebrafish spleen. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T cells and NK like and a minor one of myeloid like cells. Single cell transcriptomes are matched with FACS index sorting data GFP forward and side light scatter and dead cell staining	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2016 05 26\|ArrayExpress:E MTAB 4617	Protocols: Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	LCK 9#65	SAMEA4432167	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics	ENA first public:2016 12 01\|ENA last update:2016 09 12\|External Id:SAMEA4432167\|INSDC center alias:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC center name:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC first public:2016 12 01T17:01:25Z\|INSDC last update:2016 09 12T14:14:55Z\|INSDC status:public\|PI fluorescence:29\|Submitter Id:E MTAB 46171473686057:LCK 9#65\|age:3\|broker name:ArrayExpress\|common name:zebrafish\|developmental stage:adult\|forward scatter:36461\|genotype:heterozygous Tglck:EGFP\|gfp fluorescence:1297\|individual:2\|organism part:spleen\|plate:9\|sample name:E MTAB 46171473686057:LCK 9#65\|side scatter:37\|single cell well quality:good quality\|well:F5\|well information:single cell	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	E MTAB 46171473686057:LCK 9#65 p	LCK 9#65 p	Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	Experimental Factor: spleen:organism part\|Experimental Factor: 36461:forward scatter\|Experimental Factor: 37:side scatter\|Experimental Factor: 1297:GFP fluorescence	RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	ERP015799	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2019 01 23	LCK_9_65_1.fq LCK_9_65_2.fq	fastq fastq	88420500.0	353682.0	E MTAB 46171473686057:LCK 9 65	0:125 1:125	A:23555099;C:20818010;G:20102375;T:23882180;N:62836	125	125	23555099	20818010	20102375	23882180	62836	ERX1690928	ERS1343616	ERA701304	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	2	0.78208	0.77094	0.22912	0.22706	0.98739	0.98821	0.50449	0.50993	125	125	B	B	biological fallback assumption	illumina	hiseq_era	full_length	random_priming	unknown	sc	single_cell_plate	smartseq	Switzerland	2016-05-26	Adult	Adult	Spleen	Hematopoietic System
4193	4193	ERR1620562	ERX1690927	ERS1343615	ERP015799	PRJEB14175	Single cell RNA sequencing of spleen derived LCK cells from adult Zebrafish	E-MTAB-4617	Transcriptome Analysis	Transcriptome data from individual lck:GFP expressing cells isolated from adult Zebrafish spleen. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T cells and NK like and a minor one of myeloid like cells. Single cell transcriptomes are matched with FACS index sorting data GFP forward and side light scatter and dead cell staining	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2016 05 26\|ArrayExpress:E MTAB 4617	Protocols: Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	LCK 9#64	SAMEA4432166	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics	ENA first public:2016 12 01\|ENA last update:2016 09 12\|External Id:SAMEA4432166\|INSDC center alias:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC center name:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC first public:2016 12 01T17:01:25Z\|INSDC last update:2016 09 12T14:14:55Z\|INSDC status:public\|PI fluorescence:26\|Submitter Id:E MTAB 46171473686057:LCK 9#64\|age:3\|broker name:ArrayExpress\|common name:zebrafish\|developmental stage:adult\|forward scatter:35174\|genotype:heterozygous Tglck:EGFP\|gfp fluorescence:1140\|individual:2\|organism part:spleen\|plate:9\|sample name:E MTAB 46171473686057:LCK 9#64\|side scatter:47\|single cell well quality:good quality\|well:F4\|well information:single cell	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	E MTAB 46171473686057:LCK 9#64 p	LCK 9#64 p	Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	Experimental Factor: spleen:organism part\|Experimental Factor: 35174:forward scatter\|Experimental Factor: 47:side scatter\|Experimental Factor: 1140:GFP fluorescence	RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	ERP015799	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2018 11 16	LCK_9_64_1.fq.gz LCK_9_64_2.fq.gz	fastq fastq	345199750.0	1380799.0	E MTAB 46171473686057:LCK 9 64	0:125 1:125	A:102534015;C:70681864;G:67464984;T:104246516;N:272371	125	125	102534015	70681864	67464984	104246516	272371	ERX1690927	ERS1343615	ERA701304	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	2	0.83724	0.83578	0.27178	0.27661	0.98677	0.98689	0.61578	0.63698	125	125	B	B	biological fallback assumption	illumina	hiseq_era	full_length	random_priming	unknown	sc	single_cell_plate	smartseq	Switzerland	2016-05-26	Adult	Adult	Spleen	Hematopoietic System
4194	4194	ERR1620561	ERX1690926	ERS1343614	ERP015799	PRJEB14175	Single cell RNA sequencing of spleen derived LCK cells from adult Zebrafish	E-MTAB-4617	Transcriptome Analysis	Transcriptome data from individual lck:GFP expressing cells isolated from adult Zebrafish spleen. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T cells and NK like and a minor one of myeloid like cells. Single cell transcriptomes are matched with FACS index sorting data GFP forward and side light scatter and dead cell staining	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2016 05 26\|ArrayExpress:E MTAB 4617	Protocols: Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	LCK 9#63	SAMEA4432165	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics	ENA first public:2016 12 01\|ENA last update:2016 09 12\|External Id:SAMEA4432165\|INSDC center alias:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC center name:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC first public:2016 12 01T17:01:25Z\|INSDC last update:2016 09 12T14:14:55Z\|INSDC status:public\|PI fluorescence:17\|Submitter Id:E MTAB 46171473686057:LCK 9#63\|age:3\|broker name:ArrayExpress\|common name:zebrafish\|developmental stage:adult\|forward scatter:30162\|genotype:heterozygous Tglck:EGFP\|gfp fluorescence:481\|individual:2\|organism part:spleen\|plate:9\|sample name:E MTAB 46171473686057:LCK 9#63\|side scatter:58\|single cell well quality:good quality\|well:F3\|well information:single cell	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	E MTAB 46171473686057:LCK 9#63 p	LCK 9#63 p	Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	Experimental Factor: spleen:organism part\|Experimental Factor: 30162:forward scatter\|Experimental Factor: 58:side scatter\|Experimental Factor: 481:GFP fluorescence	RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	ERP015799	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2019 01 23	LCK_9_63_1.fq LCK_9_63_2.fq	fastq fastq	937599500.0	3750398.0	E MTAB 46171473686057:LCK 9 63	0:125 1:125	A:285752424;C:184050097;G:173746752;T:293084604;N:965623	125	125	285752424	184050097	173746752	293084604	965623	ERX1690926	ERS1343614	ERA701304	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	2	0.87975	0.88057	0.33088	0.33334	0.98884	0.989	0.58797	0.62896	125	125	B	B	biological fallback assumption	illumina	hiseq_era	full_length	random_priming	unknown	sc	single_cell_plate	smartseq	Switzerland	2016-05-26	Adult	Adult	Spleen	Hematopoietic System
4195	4195	ERR1620560	ERX1690925	ERS1343613	ERP015799	PRJEB14175	Single cell RNA sequencing of spleen derived LCK cells from adult Zebrafish	E-MTAB-4617	Transcriptome Analysis	Transcriptome data from individual lck:GFP expressing cells isolated from adult Zebrafish spleen. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T cells and NK like and a minor one of myeloid like cells. Single cell transcriptomes are matched with FACS index sorting data GFP forward and side light scatter and dead cell staining	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2016 05 26\|ArrayExpress:E MTAB 4617	Protocols: Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	LCK 9#62	SAMEA4432164	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics	ENA first public:2016 12 01\|ENA last update:2016 09 12\|External Id:SAMEA4432164\|INSDC center alias:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC center name:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC first public:2016 12 01T17:01:25Z\|INSDC last update:2016 09 12T14:14:55Z\|INSDC status:public\|PI fluorescence:22\|Submitter Id:E MTAB 46171473686057:LCK 9#62\|age:3\|broker name:ArrayExpress\|common name:zebrafish\|developmental stage:adult\|forward scatter:37186\|genotype:heterozygous Tglck:EGFP\|gfp fluorescence:812\|individual:2\|organism part:spleen\|plate:9\|sample name:E MTAB 46171473686057:LCK 9#62\|side scatter:63\|single cell well quality:good quality\|well:F2\|well information:single cell	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	E MTAB 46171473686057:LCK 9#62 p	LCK 9#62 p	Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	Experimental Factor: spleen:organism part\|Experimental Factor: 37186:forward scatter\|Experimental Factor: 63:side scatter\|Experimental Factor: 812:GFP fluorescence	RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	ERP015799	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2019 01 23	LCK_9_62_1.fq LCK_9_62_2.fq	fastq fastq	170587500.0	682350.0	E MTAB 46171473686057:LCK 9 62	0:125 1:125	A:48833185;C:36775374;G:35641739;T:49208992;N:128210	125	125	48833185	36775374	35641739	49208992	128210	ERX1690925	ERS1343613	ERA701304	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	2	0.77584	0.77392	0.23937	0.23925	0.98845	0.98879	0.48105	0.49164	125	125	B	B	biological fallback assumption	illumina	hiseq_era	full_length	random_priming	unknown	sc	single_cell_plate	smartseq	Switzerland	2016-05-26	Adult	Adult	Spleen	Hematopoietic System
4196	4196	ERR1620559	ERX1690924	ERS1343612	ERP015799	PRJEB14175	Single cell RNA sequencing of spleen derived LCK cells from adult Zebrafish	E-MTAB-4617	Transcriptome Analysis	Transcriptome data from individual lck:GFP expressing cells isolated from adult Zebrafish spleen. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T cells and NK like and a minor one of myeloid like cells. Single cell transcriptomes are matched with FACS index sorting data GFP forward and side light scatter and dead cell staining	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2016 05 26\|ArrayExpress:E MTAB 4617	Protocols: Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	LCK 9#61	SAMEA4432163	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics	ENA first public:2016 12 01\|ENA last update:2016 09 12\|External Id:SAMEA4432163\|INSDC center alias:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC center name:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC first public:2016 12 01T17:01:25Z\|INSDC last update:2016 09 12T14:14:55Z\|INSDC status:public\|PI fluorescence:11\|Submitter Id:E MTAB 46171473686057:LCK 9#61\|age:3\|broker name:ArrayExpress\|common name:zebrafish\|developmental stage:adult\|forward scatter:31283\|genotype:heterozygous Tglck:EGFP\|gfp fluorescence:268\|individual:2\|organism part:spleen\|plate:9\|sample name:E MTAB 46171473686057:LCK 9#61\|side scatter:58\|single cell well quality:good quality\|well:F1\|well information:single cell	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	E MTAB 46171473686057:LCK 9#61 p	LCK 9#61 p	Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	Experimental Factor: spleen:organism part\|Experimental Factor: 31283:forward scatter\|Experimental Factor: 58:side scatter\|Experimental Factor: 268:GFP fluorescence	RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	ERP015799	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2019 01 23	LCK_9_61_1.fq LCK_9_61_2.fq	fastq fastq	70364000.0	281456.0	E MTAB 46171473686057:LCK 9 61	0:125 1:125	A:19661346;C:15846450;G:14730150;T:20070763;N:55291	125	125	19661346	15846450	14730150	20070763	55291	ERX1690924	ERS1343612	ERA701304	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	2	0.64891	0.64123	0.20112	0.19932	0.98906	0.98997	0.45416	0.46458	125	125	B	B	biological fallback assumption	illumina	hiseq_era	full_length	random_priming	unknown	sc	single_cell_plate	smartseq	Switzerland	2016-05-26	Adult	Adult	Spleen	Hematopoietic System
4197	4197	ERR1620558	ERX1690923	ERS1343611	ERP015799	PRJEB14175	Single cell RNA sequencing of spleen derived LCK cells from adult Zebrafish	E-MTAB-4617	Transcriptome Analysis	Transcriptome data from individual lck:GFP expressing cells isolated from adult Zebrafish spleen. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T cells and NK like and a minor one of myeloid like cells. Single cell transcriptomes are matched with FACS index sorting data GFP forward and side light scatter and dead cell staining	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2016 05 26\|ArrayExpress:E MTAB 4617	Protocols: Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	LCK 9#60	SAMEA4432162	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics	ENA first public:2016 12 01\|ENA last update:2016 09 12\|External Id:SAMEA4432162\|INSDC center alias:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC center name:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC first public:2016 12 01T17:01:25Z\|INSDC last update:2016 09 12T14:14:55Z\|INSDC status:public\|PI fluorescence:25\|Submitter Id:E MTAB 46171473686057:LCK 9#60\|age:3\|broker name:ArrayExpress\|common name:zebrafish\|developmental stage:adult\|forward scatter:29165\|genotype:heterozygous Tglck:EGFP\|gfp fluorescence:927\|individual:2\|organism part:spleen\|plate:9\|sample name:E MTAB 46171473686057:LCK 9#60\|side scatter:22\|single cell well quality:good quality\|well:E12\|well information:single cell	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	E MTAB 46171473686057:LCK 9#60 p	LCK 9#60 p	Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	Experimental Factor: spleen:organism part\|Experimental Factor: 29165:forward scatter\|Experimental Factor: 22:side scatter\|Experimental Factor: 927:GFP fluorescence	RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	ERP015799	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2018 11 16	LCK_9_60_1.fq.gz LCK_9_60_2.fq.gz	fastq fastq	256373250.0	1025493.0	E MTAB 46171473686057:LCK 9 60	0:125 1:125	A:67888293;C:61202093;G:57308665;T:69714050;N:260149	125	125	67888293	61202093	57308665	69714050	260149	ERX1690923	ERS1343611	ERA701304	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	2	0.63022	0.62244	0.15653	0.15688	0.98496	0.98547	0.56542	0.56911	125	125	B	B	biological fallback assumption	illumina	hiseq_era	full_length	random_priming	unknown	sc	single_cell_plate	smartseq	Switzerland	2016-05-26	Adult	Adult	Spleen	Hematopoietic System
4198	4198	ERR1620557	ERX1690922	ERS1343610	ERP015799	PRJEB14175	Single cell RNA sequencing of spleen derived LCK cells from adult Zebrafish	E-MTAB-4617	Transcriptome Analysis	Transcriptome data from individual lck:GFP expressing cells isolated from adult Zebrafish spleen. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T cells and NK like and a minor one of myeloid like cells. Single cell transcriptomes are matched with FACS index sorting data GFP forward and side light scatter and dead cell staining	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2016 05 26\|ArrayExpress:E MTAB 4617	Protocols: Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	LCK 9#6	SAMEA4432161	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics	ENA first public:2016 12 01\|ENA last update:2016 09 12\|External Id:SAMEA4432161\|INSDC center alias:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC center name:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC first public:2016 12 01T17:01:25Z\|INSDC last update:2016 09 12T14:14:55Z\|INSDC status:public\|PI fluorescence:19\|Submitter Id:E MTAB 46171473686057:LCK 9#6\|age:3\|broker name:ArrayExpress\|common name:zebrafish\|developmental stage:adult\|forward scatter:32755\|genotype:heterozygous Tglck:EGFP\|gfp fluorescence:1125\|individual:2\|organism part:spleen\|plate:9\|sample name:E MTAB 46171473686057:LCK 9#6\|side scatter:38\|single cell well quality:good quality\|well:A6\|well information:single cell	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	E MTAB 46171473686057:LCK 9#6 p	LCK 9#6 p	Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	Experimental Factor: spleen:organism part\|Experimental Factor: 32755:forward scatter\|Experimental Factor: 38:side scatter\|Experimental Factor: 1125:GFP fluorescence	RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	ERP015799	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2018 11 16	LCK_9_6_1.fq.gz LCK_9_6_2.fq.gz	fastq fastq	134073750.0	536295.0	E MTAB 46171473686057:LCK 9 6	0:125 1:125	A:37489461;C:30174764;G:27861197;T:38396977;N:151351	125	125	37489461	30174764	27861197	38396977	151351	ERX1690922	ERS1343610	ERA701304	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	2	0.66609	0.65776	0.21861	0.21833	0.98445	0.98535	0.57405	0.56231	125	125	B	B	biological fallback assumption	illumina	hiseq_era	full_length	random_priming	unknown	sc	single_cell_plate	smartseq	Switzerland	2016-05-26	Adult	Adult	Spleen	Hematopoietic System
4199	4199	ERR1620556	ERX1690921	ERS1343609	ERP015799	PRJEB14175	Single cell RNA sequencing of spleen derived LCK cells from adult Zebrafish	E-MTAB-4617	Transcriptome Analysis	Transcriptome data from individual lck:GFP expressing cells isolated from adult Zebrafish spleen. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T cells and NK like and a minor one of myeloid like cells. Single cell transcriptomes are matched with FACS index sorting data GFP forward and side light scatter and dead cell staining	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2016 05 26\|ArrayExpress:E MTAB 4617	Protocols: Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	LCK 9#59	SAMEA4432160	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics	ENA first public:2016 12 01\|ENA last update:2016 09 12\|External Id:SAMEA4432160\|INSDC center alias:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC center name:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC first public:2016 12 01T17:01:25Z\|INSDC last update:2016 09 12T14:14:55Z\|INSDC status:public\|PI fluorescence:20\|Submitter Id:E MTAB 46171473686057:LCK 9#59\|age:3\|broker name:ArrayExpress\|common name:zebrafish\|developmental stage:adult\|forward scatter:38366\|genotype:heterozygous Tglck:EGFP\|gfp fluorescence:1413\|individual:2\|organism part:spleen\|plate:9\|sample name:E MTAB 46171473686057:LCK 9#59\|side scatter:39\|single cell well quality:good quality\|well:E11\|well information:single cell	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	E MTAB 46171473686057:LCK 9#59 p	LCK 9#59 p	Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	Experimental Factor: spleen:organism part\|Experimental Factor: 38366:forward scatter\|Experimental Factor: 39:side scatter\|Experimental Factor: 1413:GFP fluorescence	RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	ERP015799	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2018 11 16	LCK_9_59_1.fq.gz LCK_9_59_2.fq.gz	fastq fastq	2399458500.0	9597834.0	E MTAB 46171473686057:LCK 9 59	0:125 1:125	A:758814965;C:454492444;G:403450698;T:779615964;N:3084429	125	125	758814965	454492444	403450698	779615964	3084429	ERX1690921	ERS1343609	ERA701304	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	2	0.81346	0.81387	0.35524	0.35617	0.98871	0.98894	0.52889	0.57752	125	125	B	B	biological fallback assumption	illumina	hiseq_era	full_length	random_priming	unknown	sc	single_cell_plate	smartseq	Switzerland	2016-05-26	Adult	Adult	Spleen	Hematopoietic System
4200	4200	ERR1620555	ERX1690920	ERS1343608	ERP015799	PRJEB14175	Single cell RNA sequencing of spleen derived LCK cells from adult Zebrafish	E-MTAB-4617	Transcriptome Analysis	Transcriptome data from individual lck:GFP expressing cells isolated from adult Zebrafish spleen. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T cells and NK like and a minor one of myeloid like cells. Single cell transcriptomes are matched with FACS index sorting data GFP forward and side light scatter and dead cell staining	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2016 05 26\|ArrayExpress:E MTAB 4617	Protocols: Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	LCK 9#58	SAMEA4432159	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics	ENA first public:2016 12 01\|ENA last update:2016 09 12\|External Id:SAMEA4432159\|INSDC center alias:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC center name:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC first public:2016 12 01T17:01:25Z\|INSDC last update:2016 09 12T14:14:55Z\|INSDC status:public\|PI fluorescence:25\|Submitter Id:E MTAB 46171473686057:LCK 9#58\|age:3\|broker name:ArrayExpress\|common name:zebrafish\|developmental stage:adult\|forward scatter:37537\|genotype:heterozygous Tglck:EGFP\|gfp fluorescence:669\|individual:2\|organism part:spleen\|plate:9\|sample name:E MTAB 46171473686057:LCK 9#58\|side scatter:44\|single cell well quality:good quality\|well:E10\|well information:single cell	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	E MTAB 46171473686057:LCK 9#58 p	LCK 9#58 p	Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	Experimental Factor: spleen:organism part\|Experimental Factor: 37537:forward scatter\|Experimental Factor: 44:side scatter\|Experimental Factor: 669:GFP fluorescence	RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	ERP015799	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2018 11 16	LCK_9_58_1.fq LCK_9_58_2.fq	fastq fastq	3961744500.0	15846978.0	E MTAB 46171473686057:LCK 9 58	0:125 1:125	A:1228242214;C:782781362;G:685768880;T:1259800857;N:5151187	125	125	1228242214	782781362	685768880	1259800857	5151187	ERX1690920	ERS1343608	ERA701304	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	2	0.77803	0.78084	0.34288	0.34389	0.98557	0.98575	0.4839	0.48419	125	125	B	B	biological fallback assumption	illumina	hiseq_era	full_length	random_priming	unknown	sc	single_cell_plate	smartseq	Switzerland	2016-05-26	Adult	Adult	Spleen	Hematopoietic System
4201	4201	ERR1620554	ERX1690919	ERS1343607	ERP015799	PRJEB14175	Single cell RNA sequencing of spleen derived LCK cells from adult Zebrafish	E-MTAB-4617	Transcriptome Analysis	Transcriptome data from individual lck:GFP expressing cells isolated from adult Zebrafish spleen. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T cells and NK like and a minor one of myeloid like cells. Single cell transcriptomes are matched with FACS index sorting data GFP forward and side light scatter and dead cell staining	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2016 05 26\|ArrayExpress:E MTAB 4617	Protocols: Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	LCK 9#56	SAMEA4432158	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics	ENA first public:2016 12 01\|ENA last update:2016 09 12\|External Id:SAMEA4432158\|INSDC center alias:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC center name:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC first public:2016 12 01T17:01:25Z\|INSDC last update:2016 09 12T14:14:55Z\|INSDC status:public\|PI fluorescence:11\|Submitter Id:E MTAB 46171473686057:LCK 9#56\|age:3\|broker name:ArrayExpress\|common name:zebrafish\|developmental stage:adult\|forward scatter:38611\|genotype:heterozygous Tglck:EGFP\|gfp fluorescence:862\|individual:2\|organism part:spleen\|plate:9\|sample name:E MTAB 46171473686057:LCK 9#56\|side scatter:56\|single cell well quality:good quality\|well:E8\|well information:single cell	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	E MTAB 46171473686057:LCK 9#56 p	LCK 9#56 p	Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	Experimental Factor: spleen:organism part\|Experimental Factor: 38611:forward scatter\|Experimental Factor: 56:side scatter\|Experimental Factor: 862:GFP fluorescence	RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	ERP015799	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2018 11 16	LCK_9_56_1.fq LCK_9_56_2.fq	fastq fastq	1424484500.0	5697938.0	E MTAB 46171473686057:LCK 9 56	0:125 1:125	A:452498287;C:270162607;G:238364446;T:461649169;N:1809991	125	125	452498287	270162607	238364446	461649169	1809991	ERX1690919	ERS1343607	ERA701304	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	2	0.79823	0.79698	0.34328	0.34426	0.9867	0.98719	0.6392	0.62436	125	125	B	B	biological fallback assumption	illumina	hiseq_era	full_length	random_priming	unknown	sc	single_cell_plate	smartseq	Switzerland	2016-05-26	Adult	Adult	Spleen	Hematopoietic System
4202	4202	ERR1620553	ERX1690918	ERS1343606	ERP015799	PRJEB14175	Single cell RNA sequencing of spleen derived LCK cells from adult Zebrafish	E-MTAB-4617	Transcriptome Analysis	Transcriptome data from individual lck:GFP expressing cells isolated from adult Zebrafish spleen. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T cells and NK like and a minor one of myeloid like cells. Single cell transcriptomes are matched with FACS index sorting data GFP forward and side light scatter and dead cell staining	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2016 05 26\|ArrayExpress:E MTAB 4617	Protocols: Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	LCK 9#55	SAMEA4432157	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics	ENA first public:2016 12 01\|ENA last update:2016 09 12\|External Id:SAMEA4432157\|INSDC center alias:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC center name:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC first public:2016 12 01T17:01:25Z\|INSDC last update:2016 09 12T14:14:54Z\|INSDC status:public\|PI fluorescence:11\|Submitter Id:E MTAB 46171473686057:LCK 9#55\|age:3\|broker name:ArrayExpress\|common name:zebrafish\|developmental stage:adult\|forward scatter:33661\|genotype:heterozygous Tglck:EGFP\|gfp fluorescence:645\|individual:2\|organism part:spleen\|plate:9\|sample name:E MTAB 46171473686057:LCK 9#55\|side scatter:42\|single cell well quality:good quality\|well:E7\|well information:single cell	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	E MTAB 46171473686057:LCK 9#55 p	LCK 9#55 p	Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	Experimental Factor: spleen:organism part\|Experimental Factor: 33661:forward scatter\|Experimental Factor: 42:side scatter\|Experimental Factor: 645:GFP fluorescence	RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	ERP015799	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2018 11 16	LCK_9_55_1.fq LCK_9_55_2.fq	fastq fastq	60000.0	240.0	E MTAB 46171473686057:LCK 9 55	0:125 1:125	A:15954;C:17694;G:13370;T:12978;N:4	125	125	15954	17694	13370	12978	4	ERX1690918	ERS1343606	ERA701304	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	2	0.60387	0.51021	0.1256	0.22448	0.9988	0.99973	0.52525	0.64285	125	125	B	B	biological fallback assumption	illumina	hiseq_era	full_length	random_priming	unknown	sc	single_cell_plate	smartseq	Switzerland	2016-05-26	Adult	Adult	Spleen	Hematopoietic System
4203	4203	ERR1620552	ERX1690917	ERS1343605	ERP015799	PRJEB14175	Single cell RNA sequencing of spleen derived LCK cells from adult Zebrafish	E-MTAB-4617	Transcriptome Analysis	Transcriptome data from individual lck:GFP expressing cells isolated from adult Zebrafish spleen. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T cells and NK like and a minor one of myeloid like cells. Single cell transcriptomes are matched with FACS index sorting data GFP forward and side light scatter and dead cell staining	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2016 05 26\|ArrayExpress:E MTAB 4617	Protocols: Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	LCK 9#54	SAMEA4432156	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics	ENA first public:2016 12 01\|ENA last update:2016 09 12\|External Id:SAMEA4432156\|INSDC center alias:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC center name:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC first public:2016 12 01T17:01:25Z\|INSDC last update:2016 09 12T14:14:54Z\|INSDC status:public\|PI fluorescence:21\|Submitter Id:E MTAB 46171473686057:LCK 9#54\|age:3\|broker name:ArrayExpress\|common name:zebrafish\|developmental stage:adult\|forward scatter:36884\|genotype:heterozygous Tglck:EGFP\|gfp fluorescence:506\|individual:2\|organism part:spleen\|plate:9\|sample name:E MTAB 46171473686057:LCK 9#54\|side scatter:40\|single cell well quality:good quality\|well:E6\|well information:single cell	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	E MTAB 46171473686057:LCK 9#54 p	LCK 9#54 p	Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	Experimental Factor: spleen:organism part\|Experimental Factor: 36884:forward scatter\|Experimental Factor: 40:side scatter\|Experimental Factor: 506:GFP fluorescence	RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	ERP015799	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2018 11 16	LCK_9_54_1.fq LCK_9_54_2.fq	fastq fastq	983455000.0	3933820.0	E MTAB 46171473686057:LCK 9 54	0:125 1:125	A:307668147;C:189783625;G:169392422;T:315384487;N:1226319	125	125	307668147	189783625	169392422	315384487	1226319	ERX1690917	ERS1343605	ERA701304	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	2	0.84328	0.84671	0.38941	0.39293	0.98545	0.9861	0.58475	0.59234	125	125	B	B	biological fallback assumption	illumina	hiseq_era	full_length	random_priming	unknown	sc	single_cell_plate	smartseq	Switzerland	2016-05-26	Adult	Adult	Spleen	Hematopoietic System
4204	4204	ERR1620551	ERX1690916	ERS1343604	ERP015799	PRJEB14175	Single cell RNA sequencing of spleen derived LCK cells from adult Zebrafish	E-MTAB-4617	Transcriptome Analysis	Transcriptome data from individual lck:GFP expressing cells isolated from adult Zebrafish spleen. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T cells and NK like and a minor one of myeloid like cells. Single cell transcriptomes are matched with FACS index sorting data GFP forward and side light scatter and dead cell staining	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2016 05 26\|ArrayExpress:E MTAB 4617	Protocols: Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	LCK 9#53	SAMEA4432155	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics	ENA first public:2016 12 01\|ENA last update:2016 09 12\|External Id:SAMEA4432155\|INSDC center alias:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC center name:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC first public:2016 12 01T17:01:25Z\|INSDC last update:2016 09 12T14:14:54Z\|INSDC status:public\|PI fluorescence:12\|Submitter Id:E MTAB 46171473686057:LCK 9#53\|age:3\|broker name:ArrayExpress\|common name:zebrafish\|developmental stage:adult\|forward scatter:39297\|genotype:heterozygous Tglck:EGFP\|gfp fluorescence:980\|individual:2\|organism part:spleen\|plate:9\|sample name:E MTAB 46171473686057:LCK 9#53\|side scatter:48\|single cell well quality:good quality\|well:E5\|well information:single cell	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	E MTAB 46171473686057:LCK 9#53 p	LCK 9#53 p	Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	Experimental Factor: spleen:organism part\|Experimental Factor: 39297:forward scatter\|Experimental Factor: 48:side scatter\|Experimental Factor: 980:GFP fluorescence	RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	ERP015799	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2018 11 16	LCK_9_53_1.fq LCK_9_53_2.fq	fastq fastq	423622250.0	1694489.0	E MTAB 46171473686057:LCK 9 53	0:125 1:125	A:123960715;C:89449390;G:82194349;T:127533595;N:484201	125	125	123960715	89449390	82194349	127533595	484201	ERX1690916	ERS1343604	ERA701304	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	2	0.81004	0.81047	0.25781	0.2593	0.98545	0.98569	0.55857	0.54272	125	125	B	B	biological fallback assumption	illumina	hiseq_era	full_length	random_priming	unknown	sc	single_cell_plate	smartseq	Switzerland	2016-05-26	Adult	Adult	Spleen	Hematopoietic System
4205	4205	ERR1620550	ERX1690915	ERS1343603	ERP015799	PRJEB14175	Single cell RNA sequencing of spleen derived LCK cells from adult Zebrafish	E-MTAB-4617	Transcriptome Analysis	Transcriptome data from individual lck:GFP expressing cells isolated from adult Zebrafish spleen. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T cells and NK like and a minor one of myeloid like cells. Single cell transcriptomes are matched with FACS index sorting data GFP forward and side light scatter and dead cell staining	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2016 05 26\|ArrayExpress:E MTAB 4617	Protocols: Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	LCK 9#52	SAMEA4432154	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics	ENA first public:2016 12 01\|ENA last update:2016 09 12\|External Id:SAMEA4432154\|INSDC center alias:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC center name:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC first public:2016 12 01T17:01:25Z\|INSDC last update:2016 09 12T14:14:54Z\|INSDC status:public\|PI fluorescence:21\|Submitter Id:E MTAB 46171473686057:LCK 9#52\|age:3\|broker name:ArrayExpress\|common name:zebrafish\|developmental stage:adult\|forward scatter:36076\|genotype:heterozygous Tglck:EGFP\|gfp fluorescence:1834\|individual:2\|organism part:spleen\|plate:9\|sample name:E MTAB 46171473686057:LCK 9#52\|side scatter:35\|single cell well quality:good quality\|well:E4\|well information:single cell	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	E MTAB 46171473686057:LCK 9#52 p	LCK 9#52 p	Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	Experimental Factor: spleen:organism part\|Experimental Factor: 36076:forward scatter\|Experimental Factor: 35:side scatter\|Experimental Factor: 1834:GFP fluorescence	RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	ERP015799	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2019 01 23	LCK_9_52_1.fq LCK_9_52_2.fq	fastq fastq	556625750.0	2226503.0	E MTAB 46171473686057:LCK 9 52	0:125 1:125	A:170233085;C:112086841;G:98890283;T:174754179;N:661362	125	125	170233085	112086841	98890283	174754179	661362	ERX1690915	ERS1343603	ERA701304	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	2	0.79634	0.79843	0.30561	0.30767	0.98547	0.98543	0.54723	0.54948	125	125	B	B	biological fallback assumption	illumina	hiseq_era	full_length	random_priming	unknown	sc	single_cell_plate	smartseq	Switzerland	2016-05-26	Adult	Adult	Spleen	Hematopoietic System
4206	4206	ERR1620549	ERX1690914	ERS1343602	ERP015799	PRJEB14175	Single cell RNA sequencing of spleen derived LCK cells from adult Zebrafish	E-MTAB-4617	Transcriptome Analysis	Transcriptome data from individual lck:GFP expressing cells isolated from adult Zebrafish spleen. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T cells and NK like and a minor one of myeloid like cells. Single cell transcriptomes are matched with FACS index sorting data GFP forward and side light scatter and dead cell staining	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2016 05 26\|ArrayExpress:E MTAB 4617	Protocols: Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	LCK 9#51	SAMEA4432153	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics	ENA first public:2016 12 01\|ENA last update:2016 09 12\|External Id:SAMEA4432153\|INSDC center alias:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC center name:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC first public:2016 12 01T17:01:25Z\|INSDC last update:2016 09 12T14:14:54Z\|INSDC status:public\|PI fluorescence:13\|Submitter Id:E MTAB 46171473686057:LCK 9#51\|age:3\|broker name:ArrayExpress\|common name:zebrafish\|developmental stage:adult\|forward scatter:36715\|genotype:heterozygous Tglck:EGFP\|gfp fluorescence:1279\|individual:2\|organism part:spleen\|plate:9\|sample name:E MTAB 46171473686057:LCK 9#51\|side scatter:48\|single cell well quality:good quality\|well:E3\|well information:single cell	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	E MTAB 46171473686057:LCK 9#51 p	LCK 9#51 p	Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	Experimental Factor: spleen:organism part\|Experimental Factor: 36715:forward scatter\|Experimental Factor: 48:side scatter\|Experimental Factor: 1279:GFP fluorescence	RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	ERP015799	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2018 11 16	LCK_9_51_1.fq.gz LCK_9_51_2.fq.gz	fastq fastq	1409324250.0	5637297.0	E MTAB 46171473686057:LCK 9 51	0:125 1:125	A:453719761;C:261647794;G:223013782;T:469093978;N:1848935	125	125	453719761	261647794	223013782	469093978	1848935	ERX1690914	ERS1343602	ERA701304	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	2	0.8014	0.80261	0.35881	0.35948	0.98912	0.98924	0.56925	0.58077	125	125	B	B	biological fallback assumption	illumina	hiseq_era	full_length	random_priming	unknown	sc	single_cell_plate	smartseq	Switzerland	2016-05-26	Adult	Adult	Spleen	Hematopoietic System
4207	4207	ERR1620548	ERX1690913	ERS1343601	ERP015799	PRJEB14175	Single cell RNA sequencing of spleen derived LCK cells from adult Zebrafish	E-MTAB-4617	Transcriptome Analysis	Transcriptome data from individual lck:GFP expressing cells isolated from adult Zebrafish spleen. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T cells and NK like and a minor one of myeloid like cells. Single cell transcriptomes are matched with FACS index sorting data GFP forward and side light scatter and dead cell staining	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2016 05 26\|ArrayExpress:E MTAB 4617	Protocols: Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	LCK 9#50	SAMEA4432152	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics	ENA first public:2016 12 01\|ENA last update:2016 09 12\|External Id:SAMEA4432152\|INSDC center alias:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC center name:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC first public:2016 12 01T17:01:25Z\|INSDC last update:2016 09 12T14:14:54Z\|INSDC status:public\|PI fluorescence:29\|Submitter Id:E MTAB 46171473686057:LCK 9#50\|age:3\|broker name:ArrayExpress\|common name:zebrafish\|developmental stage:adult\|forward scatter:31005\|genotype:heterozygous Tglck:EGFP\|gfp fluorescence:985\|individual:2\|organism part:spleen\|plate:9\|sample name:E MTAB 46171473686057:LCK 9#50\|side scatter:67\|single cell well quality:good quality\|well:E2\|well information:single cell	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	E MTAB 46171473686057:LCK 9#50 p	LCK 9#50 p	Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	Experimental Factor: spleen:organism part\|Experimental Factor: 31005:forward scatter\|Experimental Factor: 67:side scatter\|Experimental Factor: 985:GFP fluorescence	RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	ERP015799	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2019 01 23	LCK_9_50_1.fq LCK_9_50_2.fq	fastq fastq	278424000.0	1113696.0	E MTAB 46171473686057:LCK 9 50	0:125 1:125	A:83482909;C:57129466;G:51832506;T:85657242;N:321877	125	125	83482909	57129466	51832506	85657242	321877	ERX1690913	ERS1343601	ERA701304	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	2	0.7681	0.76765	0.26779	0.26956	0.98758	0.98798	0.5507	0.56164	125	125	B	B	biological fallback assumption	illumina	hiseq_era	full_length	random_priming	unknown	sc	single_cell_plate	smartseq	Switzerland	2016-05-26	Adult	Adult	Spleen	Hematopoietic System
4208	4208	ERR1620547	ERX1690912	ERS1343600	ERP015799	PRJEB14175	Single cell RNA sequencing of spleen derived LCK cells from adult Zebrafish	E-MTAB-4617	Transcriptome Analysis	Transcriptome data from individual lck:GFP expressing cells isolated from adult Zebrafish spleen. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T cells and NK like and a minor one of myeloid like cells. Single cell transcriptomes are matched with FACS index sorting data GFP forward and side light scatter and dead cell staining	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2016 05 26\|ArrayExpress:E MTAB 4617	Protocols: Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	LCK 9#5	SAMEA4432151	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics	ENA first public:2016 12 01\|ENA last update:2016 09 12\|External Id:SAMEA4432151\|INSDC center alias:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC center name:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC first public:2016 12 01T17:01:25Z\|INSDC last update:2016 09 12T14:14:54Z\|INSDC status:public\|PI fluorescence:22\|Submitter Id:E MTAB 46171473686057:LCK 9#5\|age:3\|broker name:ArrayExpress\|common name:zebrafish\|developmental stage:adult\|forward scatter:38239\|genotype:heterozygous Tglck:EGFP\|gfp fluorescence:1331\|individual:2\|organism part:spleen\|plate:9\|sample name:E MTAB 46171473686057:LCK 9#5\|side scatter:40\|single cell well quality:good quality\|well:A5\|well information:single cell	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	E MTAB 46171473686057:LCK 9#5 p	LCK 9#5 p	Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	Experimental Factor: spleen:organism part\|Experimental Factor: 38239:forward scatter\|Experimental Factor: 40:side scatter\|Experimental Factor: 1331:GFP fluorescence	RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	ERP015799	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2018 11 16	LCK_9_5_1.fq.gz LCK_9_5_2.fq.gz	fastq fastq	68965000.0	275860.0	E MTAB 46171473686057:LCK 9 5	0:125 1:125	A:18518737;C:16248138;G:15108765;T:19013012;N:76348	125	125	18518737	16248138	15108765	19013012	76348	ERX1690912	ERS1343600	ERA701304	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	2	0.6317	0.62761	0.16172	0.16296	0.98808	0.98861	0.60645	0.61138	125	125	B	B	biological fallback assumption	illumina	hiseq_era	full_length	random_priming	unknown	sc	single_cell_plate	smartseq	Switzerland	2016-05-26	Adult	Adult	Spleen	Hematopoietic System
4209	4209	ERR1620546	ERX1690911	ERS1343599	ERP015799	PRJEB14175	Single cell RNA sequencing of spleen derived LCK cells from adult Zebrafish	E-MTAB-4617	Transcriptome Analysis	Transcriptome data from individual lck:GFP expressing cells isolated from adult Zebrafish spleen. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T cells and NK like and a minor one of myeloid like cells. Single cell transcriptomes are matched with FACS index sorting data GFP forward and side light scatter and dead cell staining	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2016 05 26\|ArrayExpress:E MTAB 4617	Protocols: Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	LCK 9#49	SAMEA4432150	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics	ENA first public:2016 12 01\|ENA last update:2016 09 12\|External Id:SAMEA4432150\|INSDC center alias:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC center name:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC first public:2016 12 01T17:01:25Z\|INSDC last update:2016 09 12T14:14:54Z\|INSDC status:public\|PI fluorescence:20\|Submitter Id:E MTAB 46171473686057:LCK 9#49\|age:3\|broker name:ArrayExpress\|common name:zebrafish\|developmental stage:adult\|forward scatter:34890\|genotype:heterozygous Tglck:EGFP\|gfp fluorescence:1206\|individual:2\|organism part:spleen\|plate:9\|sample name:E MTAB 46171473686057:LCK 9#49\|side scatter:59\|single cell well quality:good quality\|well:E1\|well information:single cell	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	E MTAB 46171473686057:LCK 9#49 p	LCK 9#49 p	Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	Experimental Factor: spleen:organism part\|Experimental Factor: 34890:forward scatter\|Experimental Factor: 59:side scatter\|Experimental Factor: 1206:GFP fluorescence	RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	ERP015799	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2018 11 16	LCK_9_49_1.fq.gz LCK_9_49_2.fq.gz	fastq fastq	136923000.0	547692.0	E MTAB 46171473686057:LCK 9 49	0:125 1:125	A:38500088;C:31023318;G:27760407;T:39504944;N:134243	125	125	38500088	31023318	27760407	39504944	134243	ERX1690911	ERS1343599	ERA701304	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	2	0.66203	0.66289	0.17977	0.18144	0.98908	0.99032	0.59894	0.59504	125	125	B	B	biological fallback assumption	illumina	hiseq_era	full_length	random_priming	unknown	sc	single_cell_plate	smartseq	Switzerland	2016-05-26	Adult	Adult	Spleen	Hematopoietic System
4210	4210	ERR1620545	ERX1690910	ERS1343598	ERP015799	PRJEB14175	Single cell RNA sequencing of spleen derived LCK cells from adult Zebrafish	E-MTAB-4617	Transcriptome Analysis	Transcriptome data from individual lck:GFP expressing cells isolated from adult Zebrafish spleen. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T cells and NK like and a minor one of myeloid like cells. Single cell transcriptomes are matched with FACS index sorting data GFP forward and side light scatter and dead cell staining	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2016 05 26\|ArrayExpress:E MTAB 4617	Protocols: Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	LCK 9#48	SAMEA4432149	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics	ENA first public:2016 12 01\|ENA last update:2016 09 12\|External Id:SAMEA4432149\|INSDC center alias:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC center name:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC first public:2016 12 01T17:01:25Z\|INSDC last update:2016 09 12T14:14:54Z\|INSDC status:public\|PI fluorescence:13\|Submitter Id:E MTAB 46171473686057:LCK 9#48\|age:3\|broker name:ArrayExpress\|common name:zebrafish\|developmental stage:adult\|forward scatter:40953\|genotype:heterozygous Tglck:EGFP\|gfp fluorescence:1994\|individual:2\|organism part:spleen\|plate:9\|sample name:E MTAB 46171473686057:LCK 9#48\|side scatter:45\|single cell well quality:good quality\|well:D12\|well information:single cell	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	E MTAB 46171473686057:LCK 9#48 p	LCK 9#48 p	Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	Experimental Factor: spleen:organism part\|Experimental Factor: 40953:forward scatter\|Experimental Factor: 45:side scatter\|Experimental Factor: 1994:GFP fluorescence	RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	ERP015799	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2018 11 16	LCK_9_48_1.fq.gz LCK_9_48_2.fq.gz	fastq fastq	294000.0	1176.0	E MTAB 46171473686057:LCK 9 48	0:125 1:125	A:77943;C:76156;G:65330;T:74347;N:224	125	125	77943	76156	65330	74347	224	ERX1690910	ERS1343598	ERA701304	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	2	0.60103	0.54121	0.09462	0.07349	0.99579	0.99732	0.52061	0.58737	125	125	B	B	biological fallback assumption	illumina	hiseq_era	full_length	random_priming	unknown	sc	single_cell_plate	smartseq	Switzerland	2016-05-26	Adult	Adult	Spleen	Hematopoietic System
4211	4211	ERR1620544	ERX1690909	ERS1343597	ERP015799	PRJEB14175	Single cell RNA sequencing of spleen derived LCK cells from adult Zebrafish	E-MTAB-4617	Transcriptome Analysis	Transcriptome data from individual lck:GFP expressing cells isolated from adult Zebrafish spleen. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T cells and NK like and a minor one of myeloid like cells. Single cell transcriptomes are matched with FACS index sorting data GFP forward and side light scatter and dead cell staining	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2016 05 26\|ArrayExpress:E MTAB 4617	Protocols: Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	LCK 9#47	SAMEA4432148	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics	ENA first public:2016 12 01\|ENA last update:2016 09 12\|External Id:SAMEA4432148\|INSDC center alias:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC center name:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC first public:2016 12 01T17:01:25Z\|INSDC last update:2016 09 12T14:14:54Z\|INSDC status:public\|PI fluorescence:18\|Submitter Id:E MTAB 46171473686057:LCK 9#47\|age:3\|broker name:ArrayExpress\|common name:zebrafish\|developmental stage:adult\|forward scatter:30609\|genotype:heterozygous Tglck:EGFP\|gfp fluorescence:674\|individual:2\|organism part:spleen\|plate:9\|sample name:E MTAB 46171473686057:LCK 9#47\|side scatter:46\|single cell well quality:good quality\|well:D11\|well information:single cell	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	E MTAB 46171473686057:LCK 9#47 p	LCK 9#47 p	Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	Experimental Factor: spleen:organism part\|Experimental Factor: 30609:forward scatter\|Experimental Factor: 46:side scatter\|Experimental Factor: 674:GFP fluorescence	RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	ERP015799	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2019 01 23	LCK_9_47_1.fq LCK_9_47_2.fq	fastq fastq	530733500.0	2122934.0	E MTAB 46171473686057:LCK 9 47	0:125 1:125	A:157990327;C:109634142;G:98693284;T:163801102;N:614645	125	125	157990327	109634142	98693284	163801102	614645	ERX1690909	ERS1343597	ERA701304	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	2	0.78508	0.78248	0.34694	0.35003	0.98604	0.98683	0.55859	0.5405	125	125	B	B	biological fallback assumption	illumina	hiseq_era	full_length	random_priming	unknown	sc	single_cell_plate	smartseq	Switzerland	2016-05-26	Adult	Adult	Spleen	Hematopoietic System
4212	4212	ERR1620543	ERX1690908	ERS1343596	ERP015799	PRJEB14175	Single cell RNA sequencing of spleen derived LCK cells from adult Zebrafish	E-MTAB-4617	Transcriptome Analysis	Transcriptome data from individual lck:GFP expressing cells isolated from adult Zebrafish spleen. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T cells and NK like and a minor one of myeloid like cells. Single cell transcriptomes are matched with FACS index sorting data GFP forward and side light scatter and dead cell staining	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2016 05 26\|ArrayExpress:E MTAB 4617	Protocols: Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	LCK 9#46	SAMEA4432147	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics	ENA first public:2016 12 01\|ENA last update:2016 09 12\|External Id:SAMEA4432147\|INSDC center alias:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC center name:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC first public:2016 12 01T17:01:25Z\|INSDC last update:2016 09 12T14:14:54Z\|INSDC status:public\|PI fluorescence:15\|Submitter Id:E MTAB 46171473686057:LCK 9#46\|age:3\|broker name:ArrayExpress\|common name:zebrafish\|developmental stage:adult\|forward scatter:38185\|genotype:heterozygous Tglck:EGFP\|gfp fluorescence:1638\|individual:2\|organism part:spleen\|plate:9\|sample name:E MTAB 46171473686057:LCK 9#46\|side scatter:56\|single cell well quality:good quality\|well:D10\|well information:single cell	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	E MTAB 46171473686057:LCK 9#46 p	LCK 9#46 p	Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	Experimental Factor: spleen:organism part\|Experimental Factor: 38185:forward scatter\|Experimental Factor: 56:side scatter\|Experimental Factor: 1638:GFP fluorescence	RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	ERP015799	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2018 11 16	LCK_9_46_1.fq LCK_9_46_2.fq	fastq fastq	1496341250.0	5985365.0	E MTAB 46171473686057:LCK 9 46	0:125 1:125	A:451412948;C:305739216;G:272791710;T:464537930;N:1859446	125	125	451412948	305739216	272791710	464537930	1859446	ERX1690908	ERS1343596	ERA701304	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	2	0.82158	0.82138	0.26396	0.26642	0.97922	0.97981	0.61964	0.63165	125	125	B	B	biological fallback assumption	illumina	hiseq_era	full_length	random_priming	unknown	sc	single_cell_plate	smartseq	Switzerland	2016-05-26	Adult	Adult	Spleen	Hematopoietic System
4213	4213	ERR1620542	ERX1690907	ERS1343595	ERP015799	PRJEB14175	Single cell RNA sequencing of spleen derived LCK cells from adult Zebrafish	E-MTAB-4617	Transcriptome Analysis	Transcriptome data from individual lck:GFP expressing cells isolated from adult Zebrafish spleen. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T cells and NK like and a minor one of myeloid like cells. Single cell transcriptomes are matched with FACS index sorting data GFP forward and side light scatter and dead cell staining	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2016 05 26\|ArrayExpress:E MTAB 4617	Protocols: Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	LCK 9#45	SAMEA4432146	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics	ENA first public:2016 12 01\|ENA last update:2016 09 12\|External Id:SAMEA4432146\|INSDC center alias:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC center name:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC first public:2016 12 01T17:01:25Z\|INSDC last update:2016 09 12T14:14:54Z\|INSDC status:public\|PI fluorescence:54\|Submitter Id:E MTAB 46171473686057:LCK 9#45\|age:3\|broker name:ArrayExpress\|common name:zebrafish\|developmental stage:adult\|forward scatter:33093\|genotype:heterozygous Tglck:EGFP\|gfp fluorescence:778\|individual:2\|organism part:spleen\|plate:9\|sample name:E MTAB 46171473686057:LCK 9#45\|side scatter:37\|single cell well quality:good quality\|well:D9\|well information:single cell	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	E MTAB 46171473686057:LCK 9#45 p	LCK 9#45 p	Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	Experimental Factor: spleen:organism part\|Experimental Factor: 33093:forward scatter\|Experimental Factor: 37:side scatter\|Experimental Factor: 778:GFP fluorescence	RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	ERP015799	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2018 11 16	LCK_9_45_1.fq LCK_9_45_2.fq	fastq fastq	1397063500.0	5588254.0	E MTAB 46171473686057:LCK 9 45	0:125 1:125	A:430585720;C:284148017;G:237096827;T:443530657;N:1702279	125	125	430585720	284148017	237096827	443530657	1702279	ERX1690907	ERS1343595	ERA701304	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	2	0.76709	0.76624	0.37672	0.38002	0.98701	0.9877	0.54309	0.54509	125	125	B	B	biological fallback assumption	illumina	hiseq_era	full_length	random_priming	unknown	sc	single_cell_plate	smartseq	Switzerland	2016-05-26	Adult	Adult	Spleen	Hematopoietic System
4214	4214	ERR1620541	ERX1690906	ERS1343594	ERP015799	PRJEB14175	Single cell RNA sequencing of spleen derived LCK cells from adult Zebrafish	E-MTAB-4617	Transcriptome Analysis	Transcriptome data from individual lck:GFP expressing cells isolated from adult Zebrafish spleen. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T cells and NK like and a minor one of myeloid like cells. Single cell transcriptomes are matched with FACS index sorting data GFP forward and side light scatter and dead cell staining	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2016 05 26\|ArrayExpress:E MTAB 4617	Protocols: Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	LCK 9#44	SAMEA4432145	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics	ENA first public:2016 12 01\|ENA last update:2016 09 12\|External Id:SAMEA4432145\|INSDC center alias:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC center name:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC first public:2016 12 01T17:01:25Z\|INSDC last update:2016 09 12T14:14:54Z\|INSDC status:public\|PI fluorescence:22\|Submitter Id:E MTAB 46171473686057:LCK 9#44\|age:3\|broker name:ArrayExpress\|common name:zebrafish\|developmental stage:adult\|forward scatter:33049\|genotype:heterozygous Tglck:EGFP\|gfp fluorescence:1185\|individual:2\|organism part:spleen\|plate:9\|sample name:E MTAB 46171473686057:LCK 9#44\|side scatter:51\|single cell well quality:good quality\|well:D8\|well information:single cell	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	E MTAB 46171473686057:LCK 9#44 p	LCK 9#44 p	Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	Experimental Factor: spleen:organism part\|Experimental Factor: 33049:forward scatter\|Experimental Factor: 51:side scatter\|Experimental Factor: 1185:GFP fluorescence	RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	ERP015799	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2018 11 16	LCK_9_44_1.fq LCK_9_44_2.fq	fastq fastq	739152000.0	2956608.0	E MTAB 46171473686057:LCK 9 44	0:125 1:125	A:224239217;C:149166771;G:133497123;T:231400509;N:848380	125	125	224239217	149166771	133497123	231400509	848380	ERX1690906	ERS1343594	ERA701304	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	2	0.79454	0.79298	0.30467	0.3067	0.9866	0.98681	0.54854	0.55635	125	125	B	B	biological fallback assumption	illumina	hiseq_era	full_length	random_priming	unknown	sc	single_cell_plate	smartseq	Switzerland	2016-05-26	Adult	Adult	Spleen	Hematopoietic System
4215	4215	ERR1620540	ERX1690905	ERS1343593	ERP015799	PRJEB14175	Single cell RNA sequencing of spleen derived LCK cells from adult Zebrafish	E-MTAB-4617	Transcriptome Analysis	Transcriptome data from individual lck:GFP expressing cells isolated from adult Zebrafish spleen. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T cells and NK like and a minor one of myeloid like cells. Single cell transcriptomes are matched with FACS index sorting data GFP forward and side light scatter and dead cell staining	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2016 05 26\|ArrayExpress:E MTAB 4617	Protocols: Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	LCK 9#43	SAMEA4432144	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics	ENA first public:2016 12 01\|ENA last update:2016 09 12\|External Id:SAMEA4432144\|INSDC center alias:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC center name:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC first public:2016 12 01T17:01:25Z\|INSDC last update:2016 09 12T14:14:54Z\|INSDC status:public\|PI fluorescence:29\|Submitter Id:E MTAB 46171473686057:LCK 9#43\|age:3\|broker name:ArrayExpress\|common name:zebrafish\|developmental stage:adult\|forward scatter:26795\|genotype:heterozygous Tglck:EGFP\|gfp fluorescence:1007\|individual:2\|organism part:spleen\|plate:9\|sample name:E MTAB 46171473686057:LCK 9#43\|side scatter:32\|single cell well quality:good quality\|well:D7\|well information:single cell	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	E MTAB 46171473686057:LCK 9#43 p	LCK 9#43 p	Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	Experimental Factor: spleen:organism part\|Experimental Factor: 26795:forward scatter\|Experimental Factor: 32:side scatter\|Experimental Factor: 1007:GFP fluorescence	RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	ERP015799	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2019 01 23	LCK_9_43_1.fq LCK_9_43_2.fq	fastq fastq	33500.0	134.0	E MTAB 46171473686057:LCK 9 43	0:125 1:125	A:8829;C:9980;G:8064;T:6627;N:0	125	125	8829	9980	8064	6627	0	ERX1690905	ERS1343593	ERA701304	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	2	0.72381	0.69231	0.13333	0.30769	0.99949	0.99991	0.62711	0.75	125	125	B	B	biological fallback assumption	illumina	hiseq_era	full_length	random_priming	unknown	sc	single_cell_plate	smartseq	Switzerland	2016-05-26	Adult	Adult	Spleen	Hematopoietic System
4216	4216	ERR1620539	ERX1690904	ERS1343592	ERP015799	PRJEB14175	Single cell RNA sequencing of spleen derived LCK cells from adult Zebrafish	E-MTAB-4617	Transcriptome Analysis	Transcriptome data from individual lck:GFP expressing cells isolated from adult Zebrafish spleen. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T cells and NK like and a minor one of myeloid like cells. Single cell transcriptomes are matched with FACS index sorting data GFP forward and side light scatter and dead cell staining	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2016 05 26\|ArrayExpress:E MTAB 4617	Protocols: Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	LCK 9#42	SAMEA4432143	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics	ENA first public:2016 12 01\|ENA last update:2016 09 12\|External Id:SAMEA4432143\|INSDC center alias:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC center name:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC first public:2016 12 01T17:01:25Z\|INSDC last update:2016 09 12T14:14:54Z\|INSDC status:public\|PI fluorescence:9\|Submitter Id:E MTAB 46171473686057:LCK 9#42\|age:3\|broker name:ArrayExpress\|common name:zebrafish\|developmental stage:adult\|forward scatter:31575\|genotype:heterozygous Tglck:EGFP\|gfp fluorescence:783\|individual:2\|organism part:spleen\|plate:9\|sample name:E MTAB 46171473686057:LCK 9#42\|side scatter:33\|single cell well quality:good quality\|well:D6\|well information:single cell	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	E MTAB 46171473686057:LCK 9#42 p	LCK 9#42 p	Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	Experimental Factor: spleen:organism part\|Experimental Factor: 31575:forward scatter\|Experimental Factor: 33:side scatter\|Experimental Factor: 783:GFP fluorescence	RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	ERP015799	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2018 11 16	LCK_9_42_1.fq LCK_9_42_2.fq	fastq fastq	360103000.0	1440412.0	E MTAB 46171473686057:LCK 9 42	0:125 1:125	A:105840816;C:75722986;G:69361332;T:108760079;N:417787	125	125	105840816	75722986	69361332	108760079	417787	ERX1690904	ERS1343592	ERA701304	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	2	0.78404	0.78245	0.30299	0.30656	0.98415	0.98474	0.58043	0.57455	125	125	B	B	biological fallback assumption	illumina	hiseq_era	full_length	random_priming	unknown	sc	single_cell_plate	smartseq	Switzerland	2016-05-26	Adult	Adult	Spleen	Hematopoietic System
4217	4217	ERR1620538	ERX1690903	ERS1343591	ERP015799	PRJEB14175	Single cell RNA sequencing of spleen derived LCK cells from adult Zebrafish	E-MTAB-4617	Transcriptome Analysis	Transcriptome data from individual lck:GFP expressing cells isolated from adult Zebrafish spleen. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T cells and NK like and a minor one of myeloid like cells. Single cell transcriptomes are matched with FACS index sorting data GFP forward and side light scatter and dead cell staining	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2016 05 26\|ArrayExpress:E MTAB 4617	Protocols: Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	LCK 9#41	SAMEA4432142	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics	ENA first public:2016 12 01\|ENA last update:2016 09 12\|External Id:SAMEA4432142\|INSDC center alias:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC center name:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC first public:2016 12 01T17:01:25Z\|INSDC last update:2016 09 12T14:14:54Z\|INSDC status:public\|PI fluorescence:35\|Submitter Id:E MTAB 46171473686057:LCK 9#41\|age:3\|broker name:ArrayExpress\|common name:zebrafish\|developmental stage:adult\|forward scatter:29650\|genotype:heterozygous Tglck:EGFP\|gfp fluorescence:817\|individual:2\|organism part:spleen\|plate:9\|sample name:E MTAB 46171473686057:LCK 9#41\|side scatter:47\|single cell well quality:good quality\|well:D5\|well information:single cell	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	E MTAB 46171473686057:LCK 9#41 p	LCK 9#41 p	Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	Experimental Factor: spleen:organism part\|Experimental Factor: 29650:forward scatter\|Experimental Factor: 47:side scatter\|Experimental Factor: 817:GFP fluorescence	RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	ERP015799	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2018 11 16	LCK_9_41_1.fq LCK_9_41_2.fq	fastq fastq	226287500.0	905150.0	E MTAB 46171473686057:LCK 9 41	0:125 1:125	A:64741328;C:48959781;G:45743423;T:66570263;N:272705	125	125	64741328	48959781	45743423	66570263	272705	ERX1690903	ERS1343591	ERA701304	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	2	0.79685	0.79796	0.3246	0.32864	0.98348	0.98419	0.49579	0.49399	125	125	B	B	biological fallback assumption	illumina	hiseq_era	full_length	random_priming	unknown	sc	single_cell_plate	smartseq	Switzerland	2016-05-26	Adult	Adult	Spleen	Hematopoietic System
4218	4218	ERR1620537	ERX1690902	ERS1343590	ERP015799	PRJEB14175	Single cell RNA sequencing of spleen derived LCK cells from adult Zebrafish	E-MTAB-4617	Transcriptome Analysis	Transcriptome data from individual lck:GFP expressing cells isolated from adult Zebrafish spleen. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T cells and NK like and a minor one of myeloid like cells. Single cell transcriptomes are matched with FACS index sorting data GFP forward and side light scatter and dead cell staining	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2016 05 26\|ArrayExpress:E MTAB 4617	Protocols: Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	LCK 9#40	SAMEA4432141	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics	ENA first public:2016 12 01\|ENA last update:2016 09 12\|External Id:SAMEA4432141\|INSDC center alias:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC center name:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC first public:2016 12 01T17:01:25Z\|INSDC last update:2016 09 12T14:14:54Z\|INSDC status:public\|PI fluorescence:17\|Submitter Id:E MTAB 46171473686057:LCK 9#40\|age:3\|broker name:ArrayExpress\|common name:zebrafish\|developmental stage:adult\|forward scatter:26984\|genotype:heterozygous Tglck:EGFP\|gfp fluorescence:445\|individual:2\|organism part:spleen\|plate:9\|sample name:E MTAB 46171473686057:LCK 9#40\|side scatter:43\|single cell well quality:good quality\|well:D4\|well information:single cell	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	E MTAB 46171473686057:LCK 9#40 p	LCK 9#40 p	Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	Experimental Factor: spleen:organism part\|Experimental Factor: 26984:forward scatter\|Experimental Factor: 43:side scatter\|Experimental Factor: 445:GFP fluorescence	RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	ERP015799	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2018 11 16	LCK_9_40_1.fq LCK_9_40_2.fq	fastq fastq	699132250.0	2796529.0	E MTAB 46171473686057:LCK 9 40	0:125 1:125	A:211289204;C:142897782;G:127054905;T:217073635;N:816724	125	125	211289204	142897782	127054905	217073635	816724	ERX1690902	ERS1343590	ERA701304	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	2	0.76106	0.76174	0.32385	0.32782	0.98829	0.98849	0.57698	0.56874	125	125	B	B	biological fallback assumption	illumina	hiseq_era	full_length	random_priming	unknown	sc	single_cell_plate	smartseq	Switzerland	2016-05-26	Adult	Adult	Spleen	Hematopoietic System
4219	4219	ERR1620536	ERX1690901	ERS1343589	ERP015799	PRJEB14175	Single cell RNA sequencing of spleen derived LCK cells from adult Zebrafish	E-MTAB-4617	Transcriptome Analysis	Transcriptome data from individual lck:GFP expressing cells isolated from adult Zebrafish spleen. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T cells and NK like and a minor one of myeloid like cells. Single cell transcriptomes are matched with FACS index sorting data GFP forward and side light scatter and dead cell staining	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2016 05 26\|ArrayExpress:E MTAB 4617	Protocols: Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	LCK 9#4	SAMEA4432140	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics	ENA first public:2016 12 01\|ENA last update:2016 09 12\|External Id:SAMEA4432140\|INSDC center alias:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC center name:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC first public:2016 12 01T17:01:25Z\|INSDC last update:2016 09 12T14:14:54Z\|INSDC status:public\|PI fluorescence:25\|Submitter Id:E MTAB 46171473686057:LCK 9#4\|age:3\|broker name:ArrayExpress\|common name:zebrafish\|developmental stage:adult\|forward scatter:36207\|genotype:heterozygous Tglck:EGFP\|gfp fluorescence:769\|individual:2\|organism part:spleen\|plate:9\|sample name:E MTAB 46171473686057:LCK 9#4\|side scatter:46\|single cell well quality:good quality\|well:A4\|well information:single cell	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	E MTAB 46171473686057:LCK 9#4 p	LCK 9#4 p	Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	Experimental Factor: spleen:organism part\|Experimental Factor: 36207:forward scatter\|Experimental Factor: 46:side scatter\|Experimental Factor: 769:GFP fluorescence	RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	ERP015799	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2018 11 16	LCK_9_4_1.fq LCK_9_4_2.fq	fastq fastq	84267500.0	337070.0	E MTAB 46171473686057:LCK 9 4	0:125 1:125	A:23156008;C:19615254;G:17731083;T:23674447;N:90708	125	125	23156008	19615254	17731083	23674447	90708	ERX1690901	ERS1343589	ERA701304	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	2	0.71683	0.70933	0.187	0.18755	0.98827	0.98853	0.58422	0.42234	125	125	B	B	biological fallback assumption	illumina	hiseq_era	full_length	random_priming	unknown	sc	single_cell_plate	smartseq	Switzerland	2016-05-26	Adult	Adult	Spleen	Hematopoietic System
4220	4220	ERR1620535	ERX1690900	ERS1343588	ERP015799	PRJEB14175	Single cell RNA sequencing of spleen derived LCK cells from adult Zebrafish	E-MTAB-4617	Transcriptome Analysis	Transcriptome data from individual lck:GFP expressing cells isolated from adult Zebrafish spleen. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T cells and NK like and a minor one of myeloid like cells. Single cell transcriptomes are matched with FACS index sorting data GFP forward and side light scatter and dead cell staining	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2016 05 26\|ArrayExpress:E MTAB 4617	Protocols: Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	LCK 9#39	SAMEA4432139	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics	ENA first public:2016 12 01\|ENA last update:2016 09 12\|External Id:SAMEA4432139\|INSDC center alias:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC center name:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC first public:2016 12 01T17:01:25Z\|INSDC last update:2016 09 12T14:14:54Z\|INSDC status:public\|PI fluorescence:12\|Submitter Id:E MTAB 46171473686057:LCK 9#39\|age:3\|broker name:ArrayExpress\|common name:zebrafish\|developmental stage:adult\|forward scatter:38628\|genotype:heterozygous Tglck:EGFP\|gfp fluorescence:1428\|individual:2\|organism part:spleen\|plate:9\|sample name:E MTAB 46171473686057:LCK 9#39\|side scatter:47\|single cell well quality:good quality\|well:D3\|well information:single cell	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	E MTAB 46171473686057:LCK 9#39 p	LCK 9#39 p	Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	Experimental Factor: spleen:organism part\|Experimental Factor: 38628:forward scatter\|Experimental Factor: 47:side scatter\|Experimental Factor: 1428:GFP fluorescence	RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	ERP015799	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2018 11 16	LCK_9_39_1.fq LCK_9_39_2.fq	fastq fastq	460656750.0	1842627.0	E MTAB 46171473686057:LCK 9 39	0:125 1:125	A:137606590;C:95351735;G:83324811;T:143873722;N:499892	125	125	137606590	95351735	83324811	143873722	499892	ERX1690900	ERS1343588	ERA701304	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	2	0.80021	0.79987	0.29451	0.29836	0.98662	0.98711	0.5754	0.55996	125	125	B	B	biological fallback assumption	illumina	hiseq_era	full_length	random_priming	unknown	sc	single_cell_plate	smartseq	Switzerland	2016-05-26	Adult	Adult	Spleen	Hematopoietic System
4221	4221	ERR1620534	ERX1690899	ERS1343587	ERP015799	PRJEB14175	Single cell RNA sequencing of spleen derived LCK cells from adult Zebrafish	E-MTAB-4617	Transcriptome Analysis	Transcriptome data from individual lck:GFP expressing cells isolated from adult Zebrafish spleen. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T cells and NK like and a minor one of myeloid like cells. Single cell transcriptomes are matched with FACS index sorting data GFP forward and side light scatter and dead cell staining	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2016 05 26\|ArrayExpress:E MTAB 4617	Protocols: Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	LCK 9#38	SAMEA4432138	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics	ENA first public:2016 12 01\|ENA last update:2016 09 12\|External Id:SAMEA4432138\|INSDC center alias:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC center name:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC first public:2016 12 01T17:01:25Z\|INSDC last update:2016 09 12T14:14:54Z\|INSDC status:public\|PI fluorescence:10\|Submitter Id:E MTAB 46171473686057:LCK 9#38\|age:3\|broker name:ArrayExpress\|common name:zebrafish\|developmental stage:adult\|forward scatter:35394\|genotype:heterozygous Tglck:EGFP\|gfp fluorescence:1703\|individual:2\|organism part:spleen\|plate:9\|sample name:E MTAB 46171473686057:LCK 9#38\|side scatter:51\|single cell well quality:good quality\|well:D2\|well information:single cell	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	E MTAB 46171473686057:LCK 9#38 p	LCK 9#38 p	Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	Experimental Factor: spleen:organism part\|Experimental Factor: 35394:forward scatter\|Experimental Factor: 51:side scatter\|Experimental Factor: 1703:GFP fluorescence	RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	ERP015799	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2018 11 16	LCK_9_38_1.fq LCK_9_38_2.fq	fastq fastq	168297750.0	673191.0	E MTAB 46171473686057:LCK 9 38	0:125 1:125	A:47533610;C:37705646;G:33476452;T:49399324;N:182718	125	125	47533610	37705646	33476452	49399324	182718	ERX1690899	ERS1343587	ERA701304	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	2	0.75279	0.74883	0.21822	0.22019	0.98656	0.98725	0.56848	0.54329	125	125	B	B	biological fallback assumption	illumina	hiseq_era	full_length	random_priming	unknown	sc	single_cell_plate	smartseq	Switzerland	2016-05-26	Adult	Adult	Spleen	Hematopoietic System
4222	4222	ERR1620533	ERX1690898	ERS1343586	ERP015799	PRJEB14175	Single cell RNA sequencing of spleen derived LCK cells from adult Zebrafish	E-MTAB-4617	Transcriptome Analysis	Transcriptome data from individual lck:GFP expressing cells isolated from adult Zebrafish spleen. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T cells and NK like and a minor one of myeloid like cells. Single cell transcriptomes are matched with FACS index sorting data GFP forward and side light scatter and dead cell staining	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2016 05 26\|ArrayExpress:E MTAB 4617	Protocols: Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	LCK 9#37	SAMEA4432137	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics	ENA first public:2016 12 01\|ENA last update:2016 09 12\|External Id:SAMEA4432137\|INSDC center alias:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC center name:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC first public:2016 12 01T17:01:25Z\|INSDC last update:2016 09 12T14:14:54Z\|INSDC status:public\|PI fluorescence:24\|Submitter Id:E MTAB 46171473686057:LCK 9#37\|age:3\|broker name:ArrayExpress\|common name:zebrafish\|developmental stage:adult\|forward scatter:33788\|genotype:heterozygous Tglck:EGFP\|gfp fluorescence:549\|individual:2\|organism part:spleen\|plate:9\|sample name:E MTAB 46171473686057:LCK 9#37\|side scatter:47\|single cell well quality:good quality\|well:D1\|well information:single cell	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	E MTAB 46171473686057:LCK 9#37 p	LCK 9#37 p	Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	Experimental Factor: spleen:organism part\|Experimental Factor: 33788:forward scatter\|Experimental Factor: 47:side scatter\|Experimental Factor: 549:GFP fluorescence	RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	ERP015799	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2018 11 16	LCK_9_37_1.fq LCK_9_37_2.fq	fastq fastq	177275750.0	709103.0	E MTAB 46171473686057:LCK 9 37	0:125 1:125	A:47179098;C:42509315;G:38373205;T:49031571;N:182561	125	125	47179098	42509315	38373205	49031571	182561	ERX1690898	ERS1343586	ERA701304	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	2	0.52619	0.51927	0.1435	0.14327	0.98758	0.98819	0.56862	0.39286	125	125	B	B	biological fallback assumption	illumina	hiseq_era	full_length	random_priming	unknown	sc	single_cell_plate	smartseq	Switzerland	2016-05-26	Adult	Adult	Spleen	Hematopoietic System
4223	4223	ERR1620532	ERX1690897	ERS1343585	ERP015799	PRJEB14175	Single cell RNA sequencing of spleen derived LCK cells from adult Zebrafish	E-MTAB-4617	Transcriptome Analysis	Transcriptome data from individual lck:GFP expressing cells isolated from adult Zebrafish spleen. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T cells and NK like and a minor one of myeloid like cells. Single cell transcriptomes are matched with FACS index sorting data GFP forward and side light scatter and dead cell staining	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2016 05 26\|ArrayExpress:E MTAB 4617	Protocols: Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	LCK 9#36	SAMEA4432136	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics	ENA first public:2016 12 01\|ENA last update:2016 09 12\|External Id:SAMEA4432136\|INSDC center alias:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC center name:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC first public:2016 12 01T17:01:25Z\|INSDC last update:2016 09 12T14:14:54Z\|INSDC status:public\|PI fluorescence:28\|Submitter Id:E MTAB 46171473686057:LCK 9#36\|age:3\|broker name:ArrayExpress\|common name:zebrafish\|developmental stage:adult\|forward scatter:35833\|genotype:heterozygous Tglck:EGFP\|gfp fluorescence:2444\|individual:2\|organism part:spleen\|plate:9\|sample name:E MTAB 46171473686057:LCK 9#36\|side scatter:52\|single cell well quality:good quality\|well:C12\|well information:single cell	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	E MTAB 46171473686057:LCK 9#36 p	LCK 9#36 p	Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	Experimental Factor: spleen:organism part\|Experimental Factor: 35833:forward scatter\|Experimental Factor: 52:side scatter\|Experimental Factor: 2444:GFP fluorescence	RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	ERP015799	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2019 01 23	LCK_9_36_1.fq LCK_9_36_2.fq	fastq fastq	270627500.0	1082510.0	E MTAB 46171473686057:LCK 9 36	0:125 1:125	A:73705184;C:62455677;G:58486650;T:75670336;N:309653	125	125	73705184	62455677	58486650	75670336	309653	ERX1690897	ERS1343585	ERA701304	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	2	0.71531	0.70368	0.17547	0.17418	0.98496	0.98545	0.5259	0.53914	125	125	B	B	biological fallback assumption	illumina	hiseq_era	full_length	random_priming	unknown	sc	single_cell_plate	smartseq	Switzerland	2016-05-26	Adult	Adult	Spleen	Hematopoietic System
4224	4224	ERR1620531	ERX1690896	ERS1343584	ERP015799	PRJEB14175	Single cell RNA sequencing of spleen derived LCK cells from adult Zebrafish	E-MTAB-4617	Transcriptome Analysis	Transcriptome data from individual lck:GFP expressing cells isolated from adult Zebrafish spleen. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T cells and NK like and a minor one of myeloid like cells. Single cell transcriptomes are matched with FACS index sorting data GFP forward and side light scatter and dead cell staining	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2016 05 26\|ArrayExpress:E MTAB 4617	Protocols: Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	LCK 9#35	SAMEA4432135	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics	ENA first public:2016 12 01\|ENA last update:2016 09 12\|External Id:SAMEA4432135\|INSDC center alias:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC center name:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC first public:2016 12 01T17:01:25Z\|INSDC last update:2016 09 12T14:14:54Z\|INSDC status:public\|PI fluorescence:11\|Submitter Id:E MTAB 46171473686057:LCK 9#35\|age:3\|broker name:ArrayExpress\|common name:zebrafish\|developmental stage:adult\|forward scatter:35022\|genotype:heterozygous Tglck:EGFP\|gfp fluorescence:630\|individual:2\|organism part:spleen\|plate:9\|sample name:E MTAB 46171473686057:LCK 9#35\|side scatter:33\|single cell well quality:good quality\|well:C11\|well information:single cell	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	E MTAB 46171473686057:LCK 9#35 p	LCK 9#35 p	Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	Experimental Factor: spleen:organism part\|Experimental Factor: 35022:forward scatter\|Experimental Factor: 33:side scatter\|Experimental Factor: 630:GFP fluorescence	RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	ERP015799	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2018 11 16	LCK_9_35_2.fq.gz LCK_9_35_1.fq.gz	fastq fastq	658569000.0	2634276.0	E MTAB 46171473686057:LCK 9 35	0:125 1:125	A:198911153;C:134001581;G:120480503;T:204336959;N:838804	125	125	198911153	134001581	120480503	204336959	838804	ERX1690896	ERS1343584	ERA701304	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	2	0.80156	0.7968	0.29153	0.29278	0.98638	0.98707	0.57116	0.57873	125	125	B	B	biological fallback assumption	illumina	hiseq_era	full_length	random_priming	unknown	sc	single_cell_plate	smartseq	Switzerland	2016-05-26	Adult	Adult	Spleen	Hematopoietic System
4225	4225	ERR1620530	ERX1690895	ERS1343583	ERP015799	PRJEB14175	Single cell RNA sequencing of spleen derived LCK cells from adult Zebrafish	E-MTAB-4617	Transcriptome Analysis	Transcriptome data from individual lck:GFP expressing cells isolated from adult Zebrafish spleen. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T cells and NK like and a minor one of myeloid like cells. Single cell transcriptomes are matched with FACS index sorting data GFP forward and side light scatter and dead cell staining	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2016 05 26\|ArrayExpress:E MTAB 4617	Protocols: Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	LCK 9#34	SAMEA4432134	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics	ENA first public:2016 12 01\|ENA last update:2016 09 12\|External Id:SAMEA4432134\|INSDC center alias:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC center name:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC first public:2016 12 01T17:01:25Z\|INSDC last update:2016 09 12T14:14:54Z\|INSDC status:public\|PI fluorescence:27\|Submitter Id:E MTAB 46171473686057:LCK 9#34\|age:3\|broker name:ArrayExpress\|common name:zebrafish\|developmental stage:adult\|forward scatter:36048\|genotype:heterozygous Tglck:EGFP\|gfp fluorescence:1911\|individual:2\|organism part:spleen\|plate:9\|sample name:E MTAB 46171473686057:LCK 9#34\|side scatter:50\|single cell well quality:good quality\|well:C10\|well information:single cell	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	E MTAB 46171473686057:LCK 9#34 p	LCK 9#34 p	Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	Experimental Factor: spleen:organism part\|Experimental Factor: 36048:forward scatter\|Experimental Factor: 50:side scatter\|Experimental Factor: 1911:GFP fluorescence	RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	ERP015799	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2018 11 16	LCK_9_34_1.fq.gz LCK_9_34_2.fq.gz	fastq fastq	2513867500.0	10055470.0	E MTAB 46171473686057:LCK 9 34	0:125 1:125	A:762559346;C:511666619;G:454748139;T:781404243;N:3489153	125	125	762559346	511666619	454748139	781404243	3489153	ERX1690895	ERS1343583	ERA701304	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	2	0.82318	0.82292	0.36763	0.3688	0.98468	0.9849	0.53575	0.54395	125	125	B	B	biological fallback assumption	illumina	hiseq_era	full_length	random_priming	unknown	sc	single_cell_plate	smartseq	Switzerland	2016-05-26	Adult	Adult	Spleen	Hematopoietic System
4226	4226	ERR1620529	ERX1690894	ERS1343582	ERP015799	PRJEB14175	Single cell RNA sequencing of spleen derived LCK cells from adult Zebrafish	E-MTAB-4617	Transcriptome Analysis	Transcriptome data from individual lck:GFP expressing cells isolated from adult Zebrafish spleen. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T cells and NK like and a minor one of myeloid like cells. Single cell transcriptomes are matched with FACS index sorting data GFP forward and side light scatter and dead cell staining	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2016 05 26\|ArrayExpress:E MTAB 4617	Protocols: Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	LCK 9#33	SAMEA4432133	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics	ENA first public:2016 12 01\|ENA last update:2016 09 12\|External Id:SAMEA4432133\|INSDC center alias:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC center name:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC first public:2016 12 01T17:01:25Z\|INSDC last update:2016 09 12T14:14:54Z\|INSDC status:public\|PI fluorescence:28\|Submitter Id:E MTAB 46171473686057:LCK 9#33\|age:3\|broker name:ArrayExpress\|common name:zebrafish\|developmental stage:adult\|forward scatter:35549\|genotype:heterozygous Tglck:EGFP\|gfp fluorescence:1499\|individual:2\|organism part:spleen\|plate:9\|sample name:E MTAB 46171473686057:LCK 9#33\|side scatter:55\|single cell well quality:good quality\|well:C9\|well information:single cell	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	E MTAB 46171473686057:LCK 9#33 p	LCK 9#33 p	Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	Experimental Factor: spleen:organism part\|Experimental Factor: 35549:forward scatter\|Experimental Factor: 55:side scatter\|Experimental Factor: 1499:GFP fluorescence	RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	ERP015799	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2018 11 16	LCK_9_33_1.fq.gz LCK_9_33_2.fq.gz	fastq fastq	2211875250.0	8847501.0	E MTAB 46171473686057:LCK 9 33	0:125 1:125	A:679550710;C:451865508;G:389649655;T:687918190;N:2891187	125	125	679550710	451865508	389649655	687918190	2891187	ERX1690894	ERS1343582	ERA701304	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	2	0.78428	0.77784	0.33713	0.33624	0.98914	0.98948	0.58772	0.59724	125	125	B	B	biological fallback assumption	illumina	hiseq_era	full_length	random_priming	unknown	sc	single_cell_plate	smartseq	Switzerland	2016-05-26	Adult	Adult	Spleen	Hematopoietic System
4227	4227	ERR1620528	ERX1690893	ERS1343581	ERP015799	PRJEB14175	Single cell RNA sequencing of spleen derived LCK cells from adult Zebrafish	E-MTAB-4617	Transcriptome Analysis	Transcriptome data from individual lck:GFP expressing cells isolated from adult Zebrafish spleen. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T cells and NK like and a minor one of myeloid like cells. Single cell transcriptomes are matched with FACS index sorting data GFP forward and side light scatter and dead cell staining	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2016 05 26\|ArrayExpress:E MTAB 4617	Protocols: Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	LCK 9#32	SAMEA4432132	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics	ENA first public:2016 12 01\|ENA last update:2016 09 12\|External Id:SAMEA4432132\|INSDC center alias:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC center name:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC first public:2016 12 01T17:01:25Z\|INSDC last update:2016 09 12T14:14:54Z\|INSDC status:public\|PI fluorescence:29\|Submitter Id:E MTAB 46171473686057:LCK 9#32\|age:3\|broker name:ArrayExpress\|common name:zebrafish\|developmental stage:adult\|forward scatter:37324\|genotype:heterozygous Tglck:EGFP\|gfp fluorescence:1017\|individual:2\|organism part:spleen\|plate:9\|sample name:E MTAB 46171473686057:LCK 9#32\|side scatter:39\|single cell well quality:good quality\|well:C8\|well information:single cell	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	E MTAB 46171473686057:LCK 9#32 p	LCK 9#32 p	Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	Experimental Factor: spleen:organism part\|Experimental Factor: 37324:forward scatter\|Experimental Factor: 39:side scatter\|Experimental Factor: 1017:GFP fluorescence	RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	ERP015799	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2018 11 16	LCK_9_32_1.fq.gz LCK_9_32_2.fq.gz	fastq fastq	1915874000.0	7663496.0	E MTAB 46171473686057:LCK 9 32	0:125 1:125	A:588937193;C:380844622;G:341308331;T:602169121;N:2614733	125	125	588937193	380844622	341308331	602169121	2614733	ERX1690893	ERS1343581	ERA701304	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	2	0.83864	0.837	0.33835	0.3381	0.98699	0.98729	0.60989	0.61408	125	125	B	B	biological fallback assumption	illumina	hiseq_era	full_length	random_priming	unknown	sc	single_cell_plate	smartseq	Switzerland	2016-05-26	Adult	Adult	Spleen	Hematopoietic System
4228	4228	ERR1620527	ERX1690892	ERS1343580	ERP015799	PRJEB14175	Single cell RNA sequencing of spleen derived LCK cells from adult Zebrafish	E-MTAB-4617	Transcriptome Analysis	Transcriptome data from individual lck:GFP expressing cells isolated from adult Zebrafish spleen. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T cells and NK like and a minor one of myeloid like cells. Single cell transcriptomes are matched with FACS index sorting data GFP forward and side light scatter and dead cell staining	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2016 05 26\|ArrayExpress:E MTAB 4617	Protocols: Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	LCK 9#31	SAMEA4432131	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics	ENA first public:2016 12 01\|ENA last update:2016 09 12\|External Id:SAMEA4432131\|INSDC center alias:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC center name:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC first public:2016 12 01T17:01:25Z\|INSDC last update:2016 09 12T14:14:54Z\|INSDC status:public\|PI fluorescence:20\|Submitter Id:E MTAB 46171473686057:LCK 9#31\|age:3\|broker name:ArrayExpress\|common name:zebrafish\|developmental stage:adult\|forward scatter:38254\|genotype:heterozygous Tglck:EGFP\|gfp fluorescence:958\|individual:2\|organism part:spleen\|plate:9\|sample name:E MTAB 46171473686057:LCK 9#31\|side scatter:38\|single cell well quality:good quality\|well:C7\|well information:single cell	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	E MTAB 46171473686057:LCK 9#31 p	LCK 9#31 p	Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	Experimental Factor: spleen:organism part\|Experimental Factor: 38254:forward scatter\|Experimental Factor: 38:side scatter\|Experimental Factor: 958:GFP fluorescence	RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	ERP015799	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2019 01 23	LCK_9_31_1.fq LCK_9_31_2.fq	fastq fastq	60500.0	242.0	E MTAB 46171473686057:LCK 9 31	0:125 1:125	A:14707;C:19967;G:15042;T:10784;N:0	125	125	14707	19967	15042	10784	0	ERX1690892	ERS1343580	ERA701304	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	2	0.51942	0.55556	0.0631	0.22222	0.99914	0.99981	0.60215	0.55555	125	125	B	B	biological fallback assumption	illumina	hiseq_era	full_length	random_priming	unknown	sc	single_cell_plate	smartseq	Switzerland	2016-05-26	Adult	Adult	Spleen	Hematopoietic System
4229	4229	ERR1620526	ERX1690891	ERS1343579	ERP015799	PRJEB14175	Single cell RNA sequencing of spleen derived LCK cells from adult Zebrafish	E-MTAB-4617	Transcriptome Analysis	Transcriptome data from individual lck:GFP expressing cells isolated from adult Zebrafish spleen. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T cells and NK like and a minor one of myeloid like cells. Single cell transcriptomes are matched with FACS index sorting data GFP forward and side light scatter and dead cell staining	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2016 05 26\|ArrayExpress:E MTAB 4617	Protocols: Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	LCK 9#30	SAMEA4432130	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics	ENA first public:2016 12 01\|ENA last update:2016 09 12\|External Id:SAMEA4432130\|INSDC center alias:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC center name:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC first public:2016 12 01T17:01:25Z\|INSDC last update:2016 09 12T14:14:54Z\|INSDC status:public\|PI fluorescence:30\|Submitter Id:E MTAB 46171473686057:LCK 9#30\|age:3\|broker name:ArrayExpress\|common name:zebrafish\|developmental stage:adult\|forward scatter:41607\|genotype:heterozygous Tglck:EGFP\|gfp fluorescence:1591\|individual:2\|organism part:spleen\|plate:9\|sample name:E MTAB 46171473686057:LCK 9#30\|side scatter:39\|single cell well quality:good quality\|well:C6\|well information:single cell	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	E MTAB 46171473686057:LCK 9#30 p	LCK 9#30 p	Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	Experimental Factor: spleen:organism part\|Experimental Factor: 41607:forward scatter\|Experimental Factor: 39:side scatter\|Experimental Factor: 1591:GFP fluorescence	RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	ERP015799	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2018 11 16	LCK_9_30_1.fq LCK_9_30_2.fq	fastq fastq	431943750.0	1727775.0	E MTAB 46171473686057:LCK 9 30	0:125 1:125	A:126269151;C:91668422;G:84244852;T:129225487;N:535838	125	125	126269151	91668422	84244852	129225487	535838	ERX1690891	ERS1343579	ERA701304	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	2	0.77735	0.77601	0.25855	0.26143	0.98212	0.98273	0.62475	0.63644	125	125	B	B	biological fallback assumption	illumina	hiseq_era	full_length	random_priming	unknown	sc	single_cell_plate	smartseq	Switzerland	2016-05-26	Adult	Adult	Spleen	Hematopoietic System
4230	4230	ERR1620525	ERX1690890	ERS1343578	ERP015799	PRJEB14175	Single cell RNA sequencing of spleen derived LCK cells from adult Zebrafish	E-MTAB-4617	Transcriptome Analysis	Transcriptome data from individual lck:GFP expressing cells isolated from adult Zebrafish spleen. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T cells and NK like and a minor one of myeloid like cells. Single cell transcriptomes are matched with FACS index sorting data GFP forward and side light scatter and dead cell staining	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2016 05 26\|ArrayExpress:E MTAB 4617	Protocols: Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	LCK 9#3	SAMEA4432129	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics	ENA first public:2016 12 01\|ENA last update:2016 09 12\|External Id:SAMEA4432129\|INSDC center alias:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC center name:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC first public:2016 12 01T17:01:25Z\|INSDC last update:2016 09 12T14:14:54Z\|INSDC status:public\|PI fluorescence:14\|Submitter Id:E MTAB 46171473686057:LCK 9#3\|age:3\|broker name:ArrayExpress\|common name:zebrafish\|developmental stage:adult\|forward scatter:35173\|genotype:heterozygous Tglck:EGFP\|gfp fluorescence:997\|individual:2\|organism part:spleen\|plate:9\|sample name:E MTAB 46171473686057:LCK 9#3\|side scatter:52\|single cell well quality:good quality\|well:A3\|well information:single cell	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	E MTAB 46171473686057:LCK 9#3 p	LCK 9#3 p	Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	Experimental Factor: spleen:organism part\|Experimental Factor: 35173:forward scatter\|Experimental Factor: 52:side scatter\|Experimental Factor: 997:GFP fluorescence	RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	ERP015799	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2018 11 16	LCK_9_3_1.fq LCK_9_3_2.fq	fastq fastq	53417500.0	213670.0	E MTAB 46171473686057:LCK 9 3	0:125 1:125	A:15170830;C:12021822;G:10576604;T:15585539;N:62705	125	125	15170830	12021822	10576604	15585539	62705	ERX1690890	ERS1343578	ERA701304	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	2	0.67102	0.66189	0.18604	0.18613	0.98784	0.98867	0.56931	0.55859	125	125	B	B	biological fallback assumption	illumina	hiseq_era	full_length	random_priming	unknown	sc	single_cell_plate	smartseq	Switzerland	2016-05-26	Adult	Adult	Spleen	Hematopoietic System
4231	4231	ERR1620524	ERX1690889	ERS1343577	ERP015799	PRJEB14175	Single cell RNA sequencing of spleen derived LCK cells from adult Zebrafish	E-MTAB-4617	Transcriptome Analysis	Transcriptome data from individual lck:GFP expressing cells isolated from adult Zebrafish spleen. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T cells and NK like and a minor one of myeloid like cells. Single cell transcriptomes are matched with FACS index sorting data GFP forward and side light scatter and dead cell staining	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2016 05 26\|ArrayExpress:E MTAB 4617	Protocols: Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	LCK 9#29	SAMEA4432128	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics	ENA first public:2016 12 01\|ENA last update:2016 09 12\|External Id:SAMEA4432128\|INSDC center alias:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC center name:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC first public:2016 12 01T17:01:25Z\|INSDC last update:2016 09 12T14:14:54Z\|INSDC status:public\|PI fluorescence:69\|Submitter Id:E MTAB 46171473686057:LCK 9#29\|age:3\|broker name:ArrayExpress\|common name:zebrafish\|developmental stage:adult\|forward scatter:33928\|genotype:heterozygous Tglck:EGFP\|gfp fluorescence:1010\|individual:2\|organism part:spleen\|plate:9\|sample name:E MTAB 46171473686057:LCK 9#29\|side scatter:103\|single cell well quality:good quality\|well:C5\|well information:single cell	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	E MTAB 46171473686057:LCK 9#29 p	LCK 9#29 p	Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	Experimental Factor: spleen:organism part\|Experimental Factor: 33928:forward scatter\|Experimental Factor: 103:side scatter\|Experimental Factor: 1010:GFP fluorescence	RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	ERP015799	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2018 11 16	LCK_9_29_1.fq LCK_9_29_2.fq	fastq fastq	400731000.0	1602924.0	E MTAB 46171473686057:LCK 9 29	0:125 1:125	A:116671373;C:84818936;G:79728402;T:119000154;N:512135	125	125	116671373	84818936	79728402	119000154	512135	ERX1690889	ERS1343577	ERA701304	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	2	0.817	0.81956	0.33674	0.33915	0.98401	0.98439	0.60776	0.61518	125	125	B	B	biological fallback assumption	illumina	hiseq_era	full_length	random_priming	unknown	sc	single_cell_plate	smartseq	Switzerland	2016-05-26	Adult	Adult	Spleen	Hematopoietic System
4232	4232	ERR1620523	ERX1690888	ERS1343576	ERP015799	PRJEB14175	Single cell RNA sequencing of spleen derived LCK cells from adult Zebrafish	E-MTAB-4617	Transcriptome Analysis	Transcriptome data from individual lck:GFP expressing cells isolated from adult Zebrafish spleen. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T cells and NK like and a minor one of myeloid like cells. Single cell transcriptomes are matched with FACS index sorting data GFP forward and side light scatter and dead cell staining	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2016 05 26\|ArrayExpress:E MTAB 4617	Protocols: Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	LCK 9#28	SAMEA4432127	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics	ENA first public:2016 12 01\|ENA last update:2016 09 12\|External Id:SAMEA4432127\|INSDC center alias:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC center name:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC first public:2016 12 01T17:01:25Z\|INSDC last update:2016 09 12T14:14:54Z\|INSDC status:public\|PI fluorescence:20\|Submitter Id:E MTAB 46171473686057:LCK 9#28\|age:3\|broker name:ArrayExpress\|common name:zebrafish\|developmental stage:adult\|forward scatter:37784\|genotype:heterozygous Tglck:EGFP\|gfp fluorescence:613\|individual:2\|organism part:spleen\|plate:9\|sample name:E MTAB 46171473686057:LCK 9#28\|side scatter:98\|single cell well quality:good quality\|well:C4\|well information:single cell	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	E MTAB 46171473686057:LCK 9#28 p	LCK 9#28 p	Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	Experimental Factor: spleen:organism part\|Experimental Factor: 37784:forward scatter\|Experimental Factor: 98:side scatter\|Experimental Factor: 613:GFP fluorescence	RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	ERP015799	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2018 11 16	LCK_9_28_1.fq LCK_9_28_2.fq	fastq fastq	445330500.0	1781322.0	E MTAB 46171473686057:LCK 9 28	0:125 1:125	A:131995962;C:92552781;G:85567682;T:134671551;N:542524	125	125	131995962	92552781	85567682	134671551	542524	ERX1690888	ERS1343576	ERA701304	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	2	0.84012	0.83909	0.25757	0.26024	0.97944	0.98011	0.5814	0.58785	125	125	B	B	biological fallback assumption	illumina	hiseq_era	full_length	random_priming	unknown	sc	single_cell_plate	smartseq	Switzerland	2016-05-26	Adult	Adult	Spleen	Hematopoietic System
4233	4233	ERR1620522	ERX1690887	ERS1343575	ERP015799	PRJEB14175	Single cell RNA sequencing of spleen derived LCK cells from adult Zebrafish	E-MTAB-4617	Transcriptome Analysis	Transcriptome data from individual lck:GFP expressing cells isolated from adult Zebrafish spleen. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T cells and NK like and a minor one of myeloid like cells. Single cell transcriptomes are matched with FACS index sorting data GFP forward and side light scatter and dead cell staining	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2016 05 26\|ArrayExpress:E MTAB 4617	Protocols: Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	LCK 9#27	SAMEA4432126	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics	ENA first public:2016 12 01\|ENA last update:2016 09 12\|External Id:SAMEA4432126\|INSDC center alias:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC center name:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC first public:2016 12 01T17:01:25Z\|INSDC last update:2016 09 12T14:14:54Z\|INSDC status:public\|PI fluorescence:26\|Submitter Id:E MTAB 46171473686057:LCK 9#27\|age:3\|broker name:ArrayExpress\|common name:zebrafish\|developmental stage:adult\|forward scatter:32075\|genotype:heterozygous Tglck:EGFP\|gfp fluorescence:787\|individual:2\|organism part:spleen\|plate:9\|sample name:E MTAB 46171473686057:LCK 9#27\|side scatter:30\|single cell well quality:good quality\|well:C3\|well information:single cell	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	E MTAB 46171473686057:LCK 9#27 p	LCK 9#27 p	Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	Experimental Factor: spleen:organism part\|Experimental Factor: 32075:forward scatter\|Experimental Factor: 30:side scatter\|Experimental Factor: 787:GFP fluorescence	RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	ERP015799	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2018 11 16	LCK_9_27_1.fq LCK_9_27_2.fq	fastq fastq	702894500.0	2811578.0	E MTAB 46171473686057:LCK 9 27	0:125 1:125	A:214185302;C:141638244;G:125423883;T:220699482;N:947589	125	125	214185302	141638244	125423883	220699482	947589	ERX1690887	ERS1343575	ERA701304	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	2	0.77633	0.77484	0.46607	0.46856	0.97948	0.98068	0.52536	0.50049	125	125	B	B	biological fallback assumption	illumina	hiseq_era	full_length	random_priming	unknown	sc	single_cell_plate	smartseq	Switzerland	2016-05-26	Adult	Adult	Spleen	Hematopoietic System
4234	4234	ERR1620521	ERX1690886	ERS1343574	ERP015799	PRJEB14175	Single cell RNA sequencing of spleen derived LCK cells from adult Zebrafish	E-MTAB-4617	Transcriptome Analysis	Transcriptome data from individual lck:GFP expressing cells isolated from adult Zebrafish spleen. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T cells and NK like and a minor one of myeloid like cells. Single cell transcriptomes are matched with FACS index sorting data GFP forward and side light scatter and dead cell staining	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2016 05 26\|ArrayExpress:E MTAB 4617	Protocols: Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	LCK 9#26	SAMEA4432125	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics	ENA first public:2016 12 01\|ENA last update:2016 09 12\|External Id:SAMEA4432125\|INSDC center alias:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC center name:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC first public:2016 12 01T17:01:25Z\|INSDC last update:2016 09 12T14:14:54Z\|INSDC status:public\|PI fluorescence:26\|Submitter Id:E MTAB 46171473686057:LCK 9#26\|age:3\|broker name:ArrayExpress\|common name:zebrafish\|developmental stage:adult\|forward scatter:34180\|genotype:heterozygous Tglck:EGFP\|gfp fluorescence:1344\|individual:2\|organism part:spleen\|plate:9\|sample name:E MTAB 46171473686057:LCK 9#26\|side scatter:49\|single cell well quality:good quality\|well:C2\|well information:single cell	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	E MTAB 46171473686057:LCK 9#26 p	LCK 9#26 p	Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	Experimental Factor: spleen:organism part\|Experimental Factor: 34180:forward scatter\|Experimental Factor: 49:side scatter\|Experimental Factor: 1344:GFP fluorescence	RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	ERP015799	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2018 11 16	LCK_9_26_1.fq LCK_9_26_2.fq	fastq fastq	264248500.0	1056994.0	E MTAB 46171473686057:LCK 9 26	0:125 1:125	A:76906878;C:56288108;G:52128806;T:78594639;N:330069	125	125	76906878	56288108	52128806	78594639	330069	ERX1690886	ERS1343574	ERA701304	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	2	0.76358	0.75974	0.29593	0.29677	0.9853	0.98583	0.59667	0.59946	125	125	B	B	biological fallback assumption	illumina	hiseq_era	full_length	random_priming	unknown	sc	single_cell_plate	smartseq	Switzerland	2016-05-26	Adult	Adult	Spleen	Hematopoietic System
4235	4235	ERR1620520	ERX1690885	ERS1343573	ERP015799	PRJEB14175	Single cell RNA sequencing of spleen derived LCK cells from adult Zebrafish	E-MTAB-4617	Transcriptome Analysis	Transcriptome data from individual lck:GFP expressing cells isolated from adult Zebrafish spleen. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T cells and NK like and a minor one of myeloid like cells. Single cell transcriptomes are matched with FACS index sorting data GFP forward and side light scatter and dead cell staining	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2016 05 26\|ArrayExpress:E MTAB 4617	Protocols: Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	LCK 9#25	SAMEA4432124	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics	ENA first public:2016 12 01\|ENA last update:2016 09 12\|External Id:SAMEA4432124\|INSDC center alias:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC center name:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC first public:2016 12 01T17:01:25Z\|INSDC last update:2016 09 12T14:14:54Z\|INSDC status:public\|PI fluorescence:18\|Submitter Id:E MTAB 46171473686057:LCK 9#25\|age:3\|broker name:ArrayExpress\|common name:zebrafish\|developmental stage:adult\|forward scatter:30506\|genotype:heterozygous Tglck:EGFP\|gfp fluorescence:1058\|individual:2\|organism part:spleen\|plate:9\|sample name:E MTAB 46171473686057:LCK 9#25\|side scatter:24\|single cell well quality:good quality\|well:C1\|well information:single cell	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	E MTAB 46171473686057:LCK 9#25 p	LCK 9#25 p	Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	Experimental Factor: spleen:organism part\|Experimental Factor: 30506:forward scatter\|Experimental Factor: 24:side scatter\|Experimental Factor: 1058:GFP fluorescence	RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	ERP015799	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2019 01 23	LCK_9_25_1.fq LCK_9_25_2.fq	fastq fastq	194061500.0	776246.0	E MTAB 46171473686057:LCK 9 25	0:125 1:125	A:53076597;C:45095249;G:41253858;T:54422186;N:213610	125	125	53076597	45095249	41253858	54422186	213610	ERX1690885	ERS1343573	ERA701304	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	2	0.65517	0.65108	0.1946	0.19681	0.98413	0.98472	0.595	0.59544	125	125	B	B	biological fallback assumption	illumina	hiseq_era	full_length	random_priming	unknown	sc	single_cell_plate	smartseq	Switzerland	2016-05-26	Adult	Adult	Spleen	Hematopoietic System
4236	4236	ERR1620519	ERX1690884	ERS1343572	ERP015799	PRJEB14175	Single cell RNA sequencing of spleen derived LCK cells from adult Zebrafish	E-MTAB-4617	Transcriptome Analysis	Transcriptome data from individual lck:GFP expressing cells isolated from adult Zebrafish spleen. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T cells and NK like and a minor one of myeloid like cells. Single cell transcriptomes are matched with FACS index sorting data GFP forward and side light scatter and dead cell staining	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2016 05 26\|ArrayExpress:E MTAB 4617	Protocols: Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	LCK 9#24	SAMEA4432123	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics	ENA first public:2016 12 01\|ENA last update:2016 09 12\|External Id:SAMEA4432123\|INSDC center alias:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC center name:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC first public:2016 12 01T17:01:25Z\|INSDC last update:2016 09 12T14:14:54Z\|INSDC status:public\|PI fluorescence:9\|Submitter Id:E MTAB 46171473686057:LCK 9#24\|age:3\|broker name:ArrayExpress\|common name:zebrafish\|developmental stage:adult\|forward scatter:38631\|genotype:heterozygous Tglck:EGFP\|gfp fluorescence:2299\|individual:2\|organism part:spleen\|plate:9\|sample name:E MTAB 46171473686057:LCK 9#24\|side scatter:67\|single cell well quality:good quality\|well:B12\|well information:single cell	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	E MTAB 46171473686057:LCK 9#24 p	LCK 9#24 p	Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	Experimental Factor: spleen:organism part\|Experimental Factor: 38631:forward scatter\|Experimental Factor: 67:side scatter\|Experimental Factor: 2299:GFP fluorescence	RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	ERP015799	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2019 01 23	LCK_9_24_1.fq LCK_9_24_2.fq	fastq fastq	317841250.0	1271365.0	E MTAB 46171473686057:LCK 9 24	0:125 1:125	A:87443769;C:72350386;G:68356562;T:89269081;N:421452	125	125	87443769	72350386	68356562	89269081	421452	ERX1690884	ERS1343572	ERA701304	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	2	0.765	0.7614	0.22441	0.22455	0.98405	0.98457	0.54945	0.54908	125	125	B	B	biological fallback assumption	illumina	hiseq_era	full_length	random_priming	unknown	sc	single_cell_plate	smartseq	Switzerland	2016-05-26	Adult	Adult	Spleen	Hematopoietic System
4237	4237	ERR1620518	ERX1690883	ERS1343571	ERP015799	PRJEB14175	Single cell RNA sequencing of spleen derived LCK cells from adult Zebrafish	E-MTAB-4617	Transcriptome Analysis	Transcriptome data from individual lck:GFP expressing cells isolated from adult Zebrafish spleen. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T cells and NK like and a minor one of myeloid like cells. Single cell transcriptomes are matched with FACS index sorting data GFP forward and side light scatter and dead cell staining	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2016 05 26\|ArrayExpress:E MTAB 4617	Protocols: Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	LCK 9#23	SAMEA4432122	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics	ENA first public:2016 12 01\|ENA last update:2016 09 12\|External Id:SAMEA4432122\|INSDC center alias:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC center name:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC first public:2016 12 01T17:01:25Z\|INSDC last update:2016 09 12T14:14:54Z\|INSDC status:public\|PI fluorescence:31\|Submitter Id:E MTAB 46171473686057:LCK 9#23\|age:3\|broker name:ArrayExpress\|common name:zebrafish\|developmental stage:adult\|forward scatter:34281\|genotype:heterozygous Tglck:EGFP\|gfp fluorescence:848\|individual:2\|organism part:spleen\|plate:9\|sample name:E MTAB 46171473686057:LCK 9#23\|side scatter:31\|single cell well quality:good quality\|well:B11\|well information:single cell	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	E MTAB 46171473686057:LCK 9#23 p	LCK 9#23 p	Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	Experimental Factor: spleen:organism part\|Experimental Factor: 34281:forward scatter\|Experimental Factor: 31:side scatter\|Experimental Factor: 848:GFP fluorescence	RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	ERP015799	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2019 01 23	LCK_9_23_1.fq LCK_9_23_2.fq	fastq fastq	350435500.0	1401742.0	E MTAB 46171473686057:LCK 9 23	0:125 1:125	A:99821826;C:76988893;G:71482078;T:101680832;N:461871	125	125	99821826	76988893	71482078	101680832	461871	ERX1690883	ERS1343571	ERA701304	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	2	0.67348	0.66912	0.24318	0.24341	0.99032	0.99064	0.56673	0.54529	125	125	B	B	biological fallback assumption	illumina	hiseq_era	full_length	random_priming	unknown	sc	single_cell_plate	smartseq	Switzerland	2016-05-26	Adult	Adult	Spleen	Hematopoietic System
4238	4238	ERR1620517	ERX1690882	ERS1343570	ERP015799	PRJEB14175	Single cell RNA sequencing of spleen derived LCK cells from adult Zebrafish	E-MTAB-4617	Transcriptome Analysis	Transcriptome data from individual lck:GFP expressing cells isolated from adult Zebrafish spleen. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T cells and NK like and a minor one of myeloid like cells. Single cell transcriptomes are matched with FACS index sorting data GFP forward and side light scatter and dead cell staining	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2016 05 26\|ArrayExpress:E MTAB 4617	Protocols: Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	LCK 9#22	SAMEA4432121	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics	ENA first public:2016 12 01\|ENA last update:2016 09 12\|External Id:SAMEA4432121\|INSDC center alias:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC center name:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC first public:2016 12 01T17:01:25Z\|INSDC last update:2016 09 12T14:14:54Z\|INSDC status:public\|PI fluorescence:21\|Submitter Id:E MTAB 46171473686057:LCK 9#22\|age:3\|broker name:ArrayExpress\|common name:zebrafish\|developmental stage:adult\|forward scatter:39716\|genotype:heterozygous Tglck:EGFP\|gfp fluorescence:3498\|individual:2\|organism part:spleen\|plate:9\|sample name:E MTAB 46171473686057:LCK 9#22\|side scatter:68\|single cell well quality:good quality\|well:B10\|well information:single cell	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	E MTAB 46171473686057:LCK 9#22 p	LCK 9#22 p	Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	Experimental Factor: spleen:organism part\|Experimental Factor: 39716:forward scatter\|Experimental Factor: 68:side scatter\|Experimental Factor: 3498:GFP fluorescence	RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	ERP015799	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2018 11 16	LCK_9_22_1.fq.gz LCK_9_22_2.fq.gz	fastq fastq	610625250.0	2442501.0	E MTAB 46171473686057:LCK 9 22	0:125 1:125	A:185949779;C:123158840;G:111788337;T:188835997;N:892297	125	125	185949779	123158840	111788337	188835997	892297	ERX1690882	ERS1343570	ERA701304	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	2	0.79181	0.79289	0.38976	0.39368	0.98267	0.98317	0.53398	0.54343	125	125	B	B	biological fallback assumption	illumina	hiseq_era	full_length	random_priming	unknown	sc	single_cell_plate	smartseq	Switzerland	2016-05-26	Adult	Adult	Spleen	Hematopoietic System
4239	4239	ERR1620516	ERX1690881	ERS1343569	ERP015799	PRJEB14175	Single cell RNA sequencing of spleen derived LCK cells from adult Zebrafish	E-MTAB-4617	Transcriptome Analysis	Transcriptome data from individual lck:GFP expressing cells isolated from adult Zebrafish spleen. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T cells and NK like and a minor one of myeloid like cells. Single cell transcriptomes are matched with FACS index sorting data GFP forward and side light scatter and dead cell staining	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2016 05 26\|ArrayExpress:E MTAB 4617	Protocols: Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	LCK 9#21	SAMEA4432120	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics	ENA first public:2016 12 01\|ENA last update:2016 09 12\|External Id:SAMEA4432120\|INSDC center alias:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC center name:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC first public:2016 12 01T17:01:25Z\|INSDC last update:2016 09 12T14:14:54Z\|INSDC status:public\|PI fluorescence:10\|Submitter Id:E MTAB 46171473686057:LCK 9#21\|age:3\|broker name:ArrayExpress\|common name:zebrafish\|developmental stage:adult\|forward scatter:29223\|genotype:heterozygous Tglck:EGFP\|gfp fluorescence:820\|individual:2\|organism part:spleen\|plate:9\|sample name:E MTAB 46171473686057:LCK 9#21\|side scatter:46\|single cell well quality:good quality\|well:B9\|well information:single cell	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	E MTAB 46171473686057:LCK 9#21 p	LCK 9#21 p	Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	Experimental Factor: spleen:organism part\|Experimental Factor: 29223:forward scatter\|Experimental Factor: 46:side scatter\|Experimental Factor: 820:GFP fluorescence	RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	ERP015799	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2018 11 16	LCK_9_21_1.fq.gz LCK_9_21_2.fq.gz	fastq fastq	1486186750.0	5944747.0	E MTAB 46171473686057:LCK 9 21	0:125 1:125	A:463125146;C:294854645;G:259484614;T:466566596;N:2155749	125	125	463125146	294854645	259484614	466566596	2155749	ERX1690881	ERS1343569	ERA701304	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	2	0.81356	0.81367	0.37507	0.37673	0.98561	0.98543	0.40477	0.56369	125	125	B	B	biological fallback assumption	illumina	hiseq_era	full_length	random_priming	unknown	sc	single_cell_plate	smartseq	Switzerland	2016-05-26	Adult	Adult	Spleen	Hematopoietic System
4240	4240	ERR1620515	ERX1690880	ERS1343568	ERP015799	PRJEB14175	Single cell RNA sequencing of spleen derived LCK cells from adult Zebrafish	E-MTAB-4617	Transcriptome Analysis	Transcriptome data from individual lck:GFP expressing cells isolated from adult Zebrafish spleen. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T cells and NK like and a minor one of myeloid like cells. Single cell transcriptomes are matched with FACS index sorting data GFP forward and side light scatter and dead cell staining	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2016 05 26\|ArrayExpress:E MTAB 4617	Protocols: Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	LCK 9#20	SAMEA4432119	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics	ENA first public:2016 12 01\|ENA last update:2016 09 12\|External Id:SAMEA4432119\|INSDC center alias:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC center name:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC first public:2016 12 01T17:01:25Z\|INSDC last update:2016 09 12T14:14:54Z\|INSDC status:public\|PI fluorescence:17\|Submitter Id:E MTAB 46171473686057:LCK 9#20\|age:3\|broker name:ArrayExpress\|common name:zebrafish\|developmental stage:adult\|forward scatter:37877\|genotype:heterozygous Tglck:EGFP\|gfp fluorescence:719\|individual:2\|organism part:spleen\|plate:9\|sample name:E MTAB 46171473686057:LCK 9#20\|side scatter:39\|single cell well quality:good quality\|well:B8\|well information:single cell	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	E MTAB 46171473686057:LCK 9#20 p	LCK 9#20 p	Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	Experimental Factor: spleen:organism part\|Experimental Factor: 37877:forward scatter\|Experimental Factor: 39:side scatter\|Experimental Factor: 719:GFP fluorescence	RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	ERP015799	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2018 11 16	LCK_9_20_1.fq LCK_9_20_2.fq	fastq fastq	721498750.0	2885995.0	E MTAB 46171473686057:LCK 9 20	0:125 1:125	A:218528953;C:146945928;G:133607460;T:221382175;N:1034234	125	125	218528953	146945928	133607460	221382175	1034234	ERX1690880	ERS1343568	ERA701304	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	2	0.78193	0.78415	0.33638	0.33943	0.98762	0.98804	0.60407	0.59556	125	125	B	B	biological fallback assumption	illumina	hiseq_era	full_length	random_priming	unknown	sc	single_cell_plate	smartseq	Switzerland	2016-05-26	Adult	Adult	Spleen	Hematopoietic System
4241	4241	ERR1620514	ERX1690879	ERS1343567	ERP015799	PRJEB14175	Single cell RNA sequencing of spleen derived LCK cells from adult Zebrafish	E-MTAB-4617	Transcriptome Analysis	Transcriptome data from individual lck:GFP expressing cells isolated from adult Zebrafish spleen. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T cells and NK like and a minor one of myeloid like cells. Single cell transcriptomes are matched with FACS index sorting data GFP forward and side light scatter and dead cell staining	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2016 05 26\|ArrayExpress:E MTAB 4617	Protocols: Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	LCK 9#2	SAMEA4432118	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics	ENA first public:2016 12 01\|ENA last update:2016 09 12\|External Id:SAMEA4432118\|INSDC center alias:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC center name:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC first public:2016 12 01T17:01:25Z\|INSDC last update:2016 09 12T14:14:54Z\|INSDC status:public\|PI fluorescence:10\|Submitter Id:E MTAB 46171473686057:LCK 9#2\|age:3\|broker name:ArrayExpress\|common name:zebrafish\|developmental stage:adult\|forward scatter:40605\|genotype:heterozygous Tglck:EGFP\|gfp fluorescence:769\|individual:2\|organism part:spleen\|plate:9\|sample name:E MTAB 46171473686057:LCK 9#2\|side scatter:55\|single cell well quality:good quality\|well:A2\|well information:single cell	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	E MTAB 46171473686057:LCK 9#2 p	LCK 9#2 p	Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	Experimental Factor: spleen:organism part\|Experimental Factor: 40605:forward scatter\|Experimental Factor: 55:side scatter\|Experimental Factor: 769:GFP fluorescence	RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	ERP015799	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2018 11 16	LCK_9_2_1.fq LCK_9_2_2.fq	fastq fastq	130088500.0	520354.0	E MTAB 46171473686057:LCK 9 2	0:125 1:125	A:35951139;C:29494894;G:27668066;T:36829765;N:144636	125	125	35951139	29494894	27668066	36829765	144636	ERX1690879	ERS1343567	ERA701304	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	2	0.78789	0.7845	0.27179	0.27377	0.9825	0.98234	0.56228	0.57076	125	125	B	B	biological fallback assumption	illumina	hiseq_era	full_length	random_priming	unknown	sc	single_cell_plate	smartseq	Switzerland	2016-05-26	Adult	Adult	Spleen	Hematopoietic System
4242	4242	ERR1620513	ERX1690878	ERS1343566	ERP015799	PRJEB14175	Single cell RNA sequencing of spleen derived LCK cells from adult Zebrafish	E-MTAB-4617	Transcriptome Analysis	Transcriptome data from individual lck:GFP expressing cells isolated from adult Zebrafish spleen. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T cells and NK like and a minor one of myeloid like cells. Single cell transcriptomes are matched with FACS index sorting data GFP forward and side light scatter and dead cell staining	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2016 05 26\|ArrayExpress:E MTAB 4617	Protocols: Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	LCK 9#19	SAMEA4432117	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics	ENA first public:2016 12 01\|ENA last update:2016 09 12\|External Id:SAMEA4432117\|INSDC center alias:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC center name:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC first public:2016 12 01T17:01:25Z\|INSDC last update:2016 09 12T14:14:54Z\|INSDC status:public\|PI fluorescence:16\|Submitter Id:E MTAB 46171473686057:LCK 9#19\|age:3\|broker name:ArrayExpress\|common name:zebrafish\|developmental stage:adult\|forward scatter:37031\|genotype:heterozygous Tglck:EGFP\|gfp fluorescence:893\|individual:2\|organism part:spleen\|plate:9\|sample name:E MTAB 46171473686057:LCK 9#19\|side scatter:43\|single cell well quality:good quality\|well:B7\|well information:single cell	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	E MTAB 46171473686057:LCK 9#19 p	LCK 9#19 p	Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	Experimental Factor: spleen:organism part\|Experimental Factor: 37031:forward scatter\|Experimental Factor: 43:side scatter\|Experimental Factor: 893:GFP fluorescence	RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	ERP015799	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2018 11 16	LCK_9_19_1.fq LCK_9_19_2.fq	fastq fastq	54250.0	217.0	E MTAB 46171473686057:LCK 9 19	0:125 1:125	A:12756;C:18509;G:13357;T:9595;N:33	125	125	12756	18509	13357	9595	33	ERX1690878	ERS1343566	ERA701304	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	2	0.64172	0.5	0.04278	0.07142	0.99912	0.99989	0.77777	0.83333	125	125	B	B	biological fallback assumption	illumina	hiseq_era	full_length	random_priming	unknown	sc	single_cell_plate	smartseq	Switzerland	2016-05-26	Adult	Adult	Spleen	Hematopoietic System
4243	4243	ERR1620512	ERX1690877	ERS1343565	ERP015799	PRJEB14175	Single cell RNA sequencing of spleen derived LCK cells from adult Zebrafish	E-MTAB-4617	Transcriptome Analysis	Transcriptome data from individual lck:GFP expressing cells isolated from adult Zebrafish spleen. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T cells and NK like and a minor one of myeloid like cells. Single cell transcriptomes are matched with FACS index sorting data GFP forward and side light scatter and dead cell staining	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2016 05 26\|ArrayExpress:E MTAB 4617	Protocols: Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	LCK 9#18	SAMEA4432116	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics	ENA first public:2016 12 01\|ENA last update:2016 09 12\|External Id:SAMEA4432116\|INSDC center alias:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC center name:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC first public:2016 12 01T17:01:25Z\|INSDC last update:2016 09 12T14:14:54Z\|INSDC status:public\|PI fluorescence:20\|Submitter Id:E MTAB 46171473686057:LCK 9#18\|age:3\|broker name:ArrayExpress\|common name:zebrafish\|developmental stage:adult\|forward scatter:34240\|genotype:heterozygous Tglck:EGFP\|gfp fluorescence:734\|individual:2\|organism part:spleen\|plate:9\|sample name:E MTAB 46171473686057:LCK 9#18\|side scatter:41\|single cell well quality:good quality\|well:B6\|well information:single cell	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	E MTAB 46171473686057:LCK 9#18 p	LCK 9#18 p	Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	Experimental Factor: spleen:organism part\|Experimental Factor: 34240:forward scatter\|Experimental Factor: 41:side scatter\|Experimental Factor: 734:GFP fluorescence	RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	ERP015799	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2018 11 16	LCK_9_18_1.fq LCK_9_18_2.fq	fastq fastq	375759000.0	1503036.0	E MTAB 46171473686057:LCK 9 18	0:125 1:125	A:107293403;C:82189292;G:76920626;T:108868180;N:487499	125	125	107293403	82189292	76920626	108868180	487499	ERX1690877	ERS1343565	ERA701304	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	2	0.695	0.69382	0.23471	0.2368	0.98567	0.98664	0.56936	0.56874	125	125	B	B	biological fallback assumption	illumina	hiseq_era	full_length	random_priming	unknown	sc	single_cell_plate	smartseq	Switzerland	2016-05-26	Adult	Adult	Spleen	Hematopoietic System
4244	4244	ERR1620511	ERX1690876	ERS1343564	ERP015799	PRJEB14175	Single cell RNA sequencing of spleen derived LCK cells from adult Zebrafish	E-MTAB-4617	Transcriptome Analysis	Transcriptome data from individual lck:GFP expressing cells isolated from adult Zebrafish spleen. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T cells and NK like and a minor one of myeloid like cells. Single cell transcriptomes are matched with FACS index sorting data GFP forward and side light scatter and dead cell staining	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2016 05 26\|ArrayExpress:E MTAB 4617	Protocols: Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	LCK 9#17	SAMEA4432115	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics	ENA first public:2016 12 01\|ENA last update:2016 09 12\|External Id:SAMEA4432115\|INSDC center alias:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC center name:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC first public:2016 12 01T17:01:25Z\|INSDC last update:2016 09 12T14:14:54Z\|INSDC status:public\|PI fluorescence:15\|Submitter Id:E MTAB 46171473686057:LCK 9#17\|age:3\|broker name:ArrayExpress\|common name:zebrafish\|developmental stage:adult\|forward scatter:36004\|genotype:heterozygous Tglck:EGFP\|gfp fluorescence:1038\|individual:2\|organism part:spleen\|plate:9\|sample name:E MTAB 46171473686057:LCK 9#17\|side scatter:50\|single cell well quality:good quality\|well:B5\|well information:single cell	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	E MTAB 46171473686057:LCK 9#17 p	LCK 9#17 p	Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	Experimental Factor: spleen:organism part\|Experimental Factor: 36004:forward scatter\|Experimental Factor: 50:side scatter\|Experimental Factor: 1038:GFP fluorescence	RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	ERP015799	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2018 11 16	LCK_9_17_1.fq LCK_9_17_2.fq	fastq fastq	230247250.0	920989.0	E MTAB 46171473686057:LCK 9 17	0:125 1:125	A:65688064;C:50045475;G:47193424;T:67001456;N:318831	125	125	65688064	50045475	47193424	67001456	318831	ERX1690876	ERS1343564	ERA701304	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	2	0.80376	0.80305	0.28121	0.28381	0.98658	0.98715	0.58975	0.58174	125	125	B	B	biological fallback assumption	illumina	hiseq_era	full_length	random_priming	unknown	sc	single_cell_plate	smartseq	Switzerland	2016-05-26	Adult	Adult	Spleen	Hematopoietic System
4245	4245	ERR1620510	ERX1690875	ERS1343563	ERP015799	PRJEB14175	Single cell RNA sequencing of spleen derived LCK cells from adult Zebrafish	E-MTAB-4617	Transcriptome Analysis	Transcriptome data from individual lck:GFP expressing cells isolated from adult Zebrafish spleen. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T cells and NK like and a minor one of myeloid like cells. Single cell transcriptomes are matched with FACS index sorting data GFP forward and side light scatter and dead cell staining	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2016 05 26\|ArrayExpress:E MTAB 4617	Protocols: Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	LCK 9#16	SAMEA4432114	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics	ENA first public:2016 12 01\|ENA last update:2016 09 12\|External Id:SAMEA4432114\|INSDC center alias:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC center name:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC first public:2016 12 01T17:01:25Z\|INSDC last update:2016 09 12T14:14:53Z\|INSDC status:public\|PI fluorescence:24\|Submitter Id:E MTAB 46171473686057:LCK 9#16\|age:3\|broker name:ArrayExpress\|common name:zebrafish\|developmental stage:adult\|forward scatter:37585\|genotype:heterozygous Tglck:EGFP\|gfp fluorescence:663\|individual:2\|organism part:spleen\|plate:9\|sample name:E MTAB 46171473686057:LCK 9#16\|side scatter:60\|single cell well quality:good quality\|well:B4\|well information:single cell	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	E MTAB 46171473686057:LCK 9#16 p	LCK 9#16 p	Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	Experimental Factor: spleen:organism part\|Experimental Factor: 37585:forward scatter\|Experimental Factor: 60:side scatter\|Experimental Factor: 663:GFP fluorescence	RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	ERP015799	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2018 11 16	LCK_9_16_1.fq LCK_9_16_2.fq	fastq fastq	152102250.0	608409.0	E MTAB 46171473686057:LCK 9 16	0:125 1:125	A:44312603;C:33141661;G:29639107;T:44812943;N:195936	125	125	44312603	33141661	29639107	44812943	195936	ERX1690875	ERS1343563	ERA701304	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	2	0.68299	0.68319	0.22787	0.23043	0.98788	0.98845	0.61495	0.61758	125	125	B	B	biological fallback assumption	illumina	hiseq_era	full_length	random_priming	unknown	sc	single_cell_plate	smartseq	Switzerland	2016-05-26	Adult	Adult	Spleen	Hematopoietic System
4246	4246	ERR1620509	ERX1690874	ERS1343562	ERP015799	PRJEB14175	Single cell RNA sequencing of spleen derived LCK cells from adult Zebrafish	E-MTAB-4617	Transcriptome Analysis	Transcriptome data from individual lck:GFP expressing cells isolated from adult Zebrafish spleen. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T cells and NK like and a minor one of myeloid like cells. Single cell transcriptomes are matched with FACS index sorting data GFP forward and side light scatter and dead cell staining	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2016 05 26\|ArrayExpress:E MTAB 4617	Protocols: Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	LCK 9#15	SAMEA4432113	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics	ENA first public:2016 12 01\|ENA last update:2016 09 12\|External Id:SAMEA4432113\|INSDC center alias:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC center name:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC first public:2016 12 01T17:01:25Z\|INSDC last update:2016 09 12T14:14:53Z\|INSDC status:public\|PI fluorescence:64\|Submitter Id:E MTAB 46171473686057:LCK 9#15\|age:3\|broker name:ArrayExpress\|common name:zebrafish\|developmental stage:adult\|forward scatter:39656\|genotype:heterozygous Tglck:EGFP\|gfp fluorescence:1505\|individual:2\|organism part:spleen\|plate:9\|sample name:E MTAB 46171473686057:LCK 9#15\|side scatter:98\|single cell well quality:good quality\|well:B3\|well information:single cell	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	E MTAB 46171473686057:LCK 9#15 p	LCK 9#15 p	Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	Experimental Factor: spleen:organism part\|Experimental Factor: 39656:forward scatter\|Experimental Factor: 98:side scatter\|Experimental Factor: 1505:GFP fluorescence	RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	ERP015799	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2018 11 16	LCK_9_15_1.fq LCK_9_15_2.fq	fastq fastq	266111000.0	1064444.0	E MTAB 46171473686057:LCK 9 15	0:125 1:125	A:77813259;C:56572621;G:52255374;T:79107116;N:362630	125	125	77813259	56572621	52255374	79107116	362630	ERX1690874	ERS1343562	ERA701304	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	2	0.7857	0.78669	0.2791	0.28238	0.98305	0.98356	0.55716	0.54631	125	125	B	B	biological fallback assumption	illumina	hiseq_era	full_length	random_priming	unknown	sc	single_cell_plate	smartseq	Switzerland	2016-05-26	Adult	Adult	Spleen	Hematopoietic System
4247	4247	ERR1620508	ERX1690873	ERS1343561	ERP015799	PRJEB14175	Single cell RNA sequencing of spleen derived LCK cells from adult Zebrafish	E-MTAB-4617	Transcriptome Analysis	Transcriptome data from individual lck:GFP expressing cells isolated from adult Zebrafish spleen. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T cells and NK like and a minor one of myeloid like cells. Single cell transcriptomes are matched with FACS index sorting data GFP forward and side light scatter and dead cell staining	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2016 05 26\|ArrayExpress:E MTAB 4617	Protocols: Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	LCK 9#14	SAMEA4432112	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics	ENA first public:2016 12 01\|ENA last update:2016 09 12\|External Id:SAMEA4432112\|INSDC center alias:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC center name:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC first public:2016 12 01T17:01:25Z\|INSDC last update:2016 09 12T14:14:53Z\|INSDC status:public\|PI fluorescence:22\|Submitter Id:E MTAB 46171473686057:LCK 9#14\|age:3\|broker name:ArrayExpress\|common name:zebrafish\|developmental stage:adult\|forward scatter:35756\|genotype:heterozygous Tglck:EGFP\|gfp fluorescence:910\|individual:2\|organism part:spleen\|plate:9\|sample name:E MTAB 46171473686057:LCK 9#14\|side scatter:29\|single cell well quality:good quality\|well:B2\|well information:single cell	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	E MTAB 46171473686057:LCK 9#14 p	LCK 9#14 p	Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	Experimental Factor: spleen:organism part\|Experimental Factor: 35756:forward scatter\|Experimental Factor: 29:side scatter\|Experimental Factor: 910:GFP fluorescence	RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	ERP015799	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2019 01 23	LCK_9_14_1.fq LCK_9_14_2.fq	fastq fastq	183685500.0	734742.0	E MTAB 46171473686057:LCK 9 14	0:125 1:125	A:52448302;C:40324561;G:37699565;T:52983394;N:229678	125	125	52448302	40324561	37699565	52983394	229678	ERX1690873	ERS1343561	ERA701304	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	2	0.72936	0.72964	0.22377	0.22687	0.98419	0.98466	0.58412	0.59481	125	125	B	B	biological fallback assumption	illumina	hiseq_era	full_length	random_priming	unknown	sc	single_cell_plate	smartseq	Switzerland	2016-05-26	Adult	Adult	Spleen	Hematopoietic System
4248	4248	ERR1620507	ERX1690872	ERS1343560	ERP015799	PRJEB14175	Single cell RNA sequencing of spleen derived LCK cells from adult Zebrafish	E-MTAB-4617	Transcriptome Analysis	Transcriptome data from individual lck:GFP expressing cells isolated from adult Zebrafish spleen. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T cells and NK like and a minor one of myeloid like cells. Single cell transcriptomes are matched with FACS index sorting data GFP forward and side light scatter and dead cell staining	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2016 05 26\|ArrayExpress:E MTAB 4617	Protocols: Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	LCK 9#13	SAMEA4432111	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics	ENA first public:2016 12 01\|ENA last update:2016 09 12\|External Id:SAMEA4432111\|INSDC center alias:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC center name:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC first public:2016 12 01T17:01:25Z\|INSDC last update:2016 09 12T14:14:53Z\|INSDC status:public\|PI fluorescence:35\|Submitter Id:E MTAB 46171473686057:LCK 9#13\|age:3\|broker name:ArrayExpress\|common name:zebrafish\|developmental stage:adult\|forward scatter:40047\|genotype:heterozygous Tglck:EGFP\|gfp fluorescence:552\|individual:2\|organism part:spleen\|plate:9\|sample name:E MTAB 46171473686057:LCK 9#13\|side scatter:62\|single cell well quality:good quality\|well:B1\|well information:single cell	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	E MTAB 46171473686057:LCK 9#13 p	LCK 9#13 p	Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	Experimental Factor: spleen:organism part\|Experimental Factor: 40047:forward scatter\|Experimental Factor: 62:side scatter\|Experimental Factor: 552:GFP fluorescence	RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	ERP015799	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2019 01 23	LCK_9_13_1.fq LCK_9_13_2.fq	fastq fastq	184971750.0	739887.0	E MTAB 46171473686057:LCK 9 13	0:125 1:125	A:51592242;C:42149603;G:38441744;T:52559034;N:229127	125	125	51592242	42149603	38441744	52559034	229127	ERX1690872	ERS1343560	ERA701304	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	2	0.58753	0.58435	0.21447	0.21393	0.98587	0.9866	0.51901	0.52694	125	125	B	B	biological fallback assumption	illumina	hiseq_era	full_length	random_priming	unknown	sc	single_cell_plate	smartseq	Switzerland	2016-05-26	Adult	Adult	Spleen	Hematopoietic System
4249	4249	ERR1620506	ERX1690871	ERS1343559	ERP015799	PRJEB14175	Single cell RNA sequencing of spleen derived LCK cells from adult Zebrafish	E-MTAB-4617	Transcriptome Analysis	Transcriptome data from individual lck:GFP expressing cells isolated from adult Zebrafish spleen. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T cells and NK like and a minor one of myeloid like cells. Single cell transcriptomes are matched with FACS index sorting data GFP forward and side light scatter and dead cell staining	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2016 05 26\|ArrayExpress:E MTAB 4617	Protocols: Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	LCK 9#12	SAMEA4432110	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics	ENA first public:2016 12 01\|ENA last update:2016 09 12\|External Id:SAMEA4432110\|INSDC center alias:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC center name:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC first public:2016 12 01T17:01:25Z\|INSDC last update:2016 09 12T14:14:53Z\|INSDC status:public\|PI fluorescence:17\|Submitter Id:E MTAB 46171473686057:LCK 9#12\|age:3\|broker name:ArrayExpress\|common name:zebrafish\|developmental stage:adult\|forward scatter:34674\|genotype:heterozygous Tglck:EGFP\|gfp fluorescence:1171\|individual:2\|organism part:spleen\|plate:9\|sample name:E MTAB 46171473686057:LCK 9#12\|side scatter:40\|single cell well quality:good quality\|well:A12\|well information:single cell	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	E MTAB 46171473686057:LCK 9#12 p	LCK 9#12 p	Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	Experimental Factor: spleen:organism part\|Experimental Factor: 34674:forward scatter\|Experimental Factor: 40:side scatter\|Experimental Factor: 1171:GFP fluorescence	RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	ERP015799	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2019 01 23	LCK_9_12_1.fq LCK_9_12_2.fq	fastq fastq	68500.0	274.0	E MTAB 46171473686057:LCK 9 12	0:125 1:125	A:18435;C:20110;G:15142;T:14808;N:5	125	125	18435	20110	15142	14808	5	ERX1690871	ERS1343559	ERA701304	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	2	0.53659	0.54167	0.03658	0.04166	0.9992	0.99975	0.5679	0.33333	125	125	B	B	biological fallback assumption	illumina	hiseq_era	full_length	random_priming	unknown	sc	single_cell_plate	smartseq	Switzerland	2016-05-26	Adult	Adult	Spleen	Hematopoietic System
4250	4250	ERR1620505	ERX1690870	ERS1343558	ERP015799	PRJEB14175	Single cell RNA sequencing of spleen derived LCK cells from adult Zebrafish	E-MTAB-4617	Transcriptome Analysis	Transcriptome data from individual lck:GFP expressing cells isolated from adult Zebrafish spleen. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T cells and NK like and a minor one of myeloid like cells. Single cell transcriptomes are matched with FACS index sorting data GFP forward and side light scatter and dead cell staining	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2016 05 26\|ArrayExpress:E MTAB 4617	Protocols: Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	LCK 9#11	SAMEA4432109	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics	ENA first public:2016 12 01\|ENA last update:2016 09 12\|External Id:SAMEA4432109\|INSDC center alias:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC center name:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC first public:2016 12 01T17:01:25Z\|INSDC last update:2016 09 12T14:14:53Z\|INSDC status:public\|PI fluorescence:11\|Submitter Id:E MTAB 46171473686057:LCK 9#11\|age:3\|broker name:ArrayExpress\|common name:zebrafish\|developmental stage:adult\|forward scatter:39468\|genotype:heterozygous Tglck:EGFP\|gfp fluorescence:598\|individual:2\|organism part:spleen\|plate:9\|sample name:E MTAB 46171473686057:LCK 9#11\|side scatter:59\|single cell well quality:good quality\|well:A11\|well information:single cell	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	E MTAB 46171473686057:LCK 9#11 p	LCK 9#11 p	Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	Experimental Factor: spleen:organism part\|Experimental Factor: 39468:forward scatter\|Experimental Factor: 59:side scatter\|Experimental Factor: 598:GFP fluorescence	RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	ERP015799	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2018 11 16	LCK_9_11_1.fq.gz LCK_9_11_2.fq.gz	fastq fastq	262326250.0	1049305.0	E MTAB 46171473686057:LCK 9 11	0:125 1:125	A:71255079;C:60824638;G:56934624;T:73000150;N:311759	125	125	71255079	60824638	56934624	73000150	311759	ERX1690870	ERS1343558	ERA701304	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	2	0.66736	0.65347	0.18944	0.18657	0.98673	0.9867	0.52041	0.52245	125	125	B	B	biological fallback assumption	illumina	hiseq_era	full_length	random_priming	unknown	sc	single_cell_plate	smartseq	Switzerland	2016-05-26	Adult	Adult	Spleen	Hematopoietic System
4251	4251	ERR1620504	ERX1690869	ERS1343557	ERP015799	PRJEB14175	Single cell RNA sequencing of spleen derived LCK cells from adult Zebrafish	E-MTAB-4617	Transcriptome Analysis	Transcriptome data from individual lck:GFP expressing cells isolated from adult Zebrafish spleen. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T cells and NK like and a minor one of myeloid like cells. Single cell transcriptomes are matched with FACS index sorting data GFP forward and side light scatter and dead cell staining	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2016 05 26\|ArrayExpress:E MTAB 4617	Protocols: Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	LCK 9#10	SAMEA4432108	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics	ENA first public:2016 12 01\|ENA last update:2016 09 12\|External Id:SAMEA4432108\|INSDC center alias:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC center name:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC first public:2016 12 01T17:01:25Z\|INSDC last update:2016 09 12T14:14:53Z\|INSDC status:public\|PI fluorescence:30\|Submitter Id:E MTAB 46171473686057:LCK 9#10\|age:3\|broker name:ArrayExpress\|common name:zebrafish\|developmental stage:adult\|forward scatter:40922\|genotype:heterozygous Tglck:EGFP\|gfp fluorescence:1191\|individual:2\|organism part:spleen\|plate:9\|sample name:E MTAB 46171473686057:LCK 9#10\|side scatter:47\|single cell well quality:good quality\|well:A10\|well information:single cell	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	E MTAB 46171473686057:LCK 9#10 p	LCK 9#10 p	Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	Experimental Factor: spleen:organism part\|Experimental Factor: 40922:forward scatter\|Experimental Factor: 47:side scatter\|Experimental Factor: 1191:GFP fluorescence	RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	ERP015799	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2018 11 16	LCK_9_10_1.fq.gz LCK_9_10_2.fq.gz	fastq fastq	385292750.0	1541171.0	E MTAB 46171473686057:LCK 9 10	0:125 1:125	A:106339101;C:87781231;G:81794837;T:108890761;N:486820	125	125	106339101	87781231	81794837	108890761	486820	ERX1690869	ERS1343557	ERA701304	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	2	0.72987	0.71664	0.19705	0.19603	0.98689	0.98727	0.58065	0.58479	125	125	B	B	biological fallback assumption	illumina	hiseq_era	full_length	random_priming	unknown	sc	single_cell_plate	smartseq	Switzerland	2016-05-26	Adult	Adult	Spleen	Hematopoietic System
4252	4252	ERR1620503	ERX1690868	ERS1343556	ERP015799	PRJEB14175	Single cell RNA sequencing of spleen derived LCK cells from adult Zebrafish	E-MTAB-4617	Transcriptome Analysis	Transcriptome data from individual lck:GFP expressing cells isolated from adult Zebrafish spleen. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T cells and NK like and a minor one of myeloid like cells. Single cell transcriptomes are matched with FACS index sorting data GFP forward and side light scatter and dead cell staining	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2016 05 26\|ArrayExpress:E MTAB 4617	Protocols: Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	LCK 9#1	SAMEA4432107	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics	ENA first public:2016 12 01\|ENA last update:2016 09 12\|External Id:SAMEA4432107\|INSDC center alias:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC center name:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC first public:2016 12 01T17:01:25Z\|INSDC last update:2016 09 12T14:14:53Z\|INSDC status:public\|PI fluorescence:20\|Submitter Id:E MTAB 46171473686057:LCK 9#1\|age:3\|broker name:ArrayExpress\|common name:zebrafish\|developmental stage:adult\|forward scatter:38064\|genotype:heterozygous Tglck:EGFP\|gfp fluorescence:1408\|individual:2\|organism part:spleen\|plate:9\|sample name:E MTAB 46171473686057:LCK 9#1\|side scatter:49\|single cell well quality:good quality\|well:A1\|well information:single cell	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	E MTAB 46171473686057:LCK 9#1 p	LCK 9#1 p	Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	Experimental Factor: spleen:organism part\|Experimental Factor: 38064:forward scatter\|Experimental Factor: 49:side scatter\|Experimental Factor: 1408:GFP fluorescence	RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	ERP015799	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2019 01 23	LCK_9_1_1.fq LCK_9_1_2.fq	fastq fastq	162490000.0	649960.0	E MTAB 46171473686057:LCK 9 1	0:125 1:125	A:45331628;C:36735182;G:33728117;T:46509543;N:185530	125	125	45331628	36735182	33728117	46509543	185530	ERX1690868	ERS1343556	ERA701304	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	2	0.74727	0.74298	0.24396	0.24573	0.98161	0.98141	0.49079	0.48358	125	125	B	B	biological fallback assumption	illumina	hiseq_era	full_length	random_priming	unknown	sc	single_cell_plate	smartseq	Switzerland	2016-05-26	Adult	Adult	Spleen	Hematopoietic System
4253	4253	ERR1620502	ERX1690867	ERS1343555	ERP015799	PRJEB14175	Single cell RNA sequencing of spleen derived LCK cells from adult Zebrafish	E-MTAB-4617	Transcriptome Analysis	Transcriptome data from individual lck:GFP expressing cells isolated from adult Zebrafish spleen. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T cells and NK like and a minor one of myeloid like cells. Single cell transcriptomes are matched with FACS index sorting data GFP forward and side light scatter and dead cell staining	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2016 05 26\|ArrayExpress:E MTAB 4617	Protocols: Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	LCK 8#9	SAMEA4432106	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics	ENA first public:2016 12 01\|ENA last update:2016 09 12\|External Id:SAMEA4432106\|INSDC center alias:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC center name:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC first public:2016 12 01T17:01:25Z\|INSDC last update:2016 09 12T14:14:53Z\|INSDC status:public\|PI fluorescence:13\|Submitter Id:E MTAB 46171473686057:LCK 8#9\|age:3\|broker name:ArrayExpress\|common name:zebrafish\|developmental stage:adult\|forward scatter:39150\|genotype:heterozygous Tglck:EGFP\|gfp fluorescence:480\|individual:2\|organism part:spleen\|plate:8\|sample name:E MTAB 46171473686057:LCK 8#9\|side scatter:68\|single cell well quality:good quality\|well:A9\|well information:single cell	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	E MTAB 46171473686057:LCK 8#9 p	LCK 8#9 p	Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	Experimental Factor: spleen:organism part\|Experimental Factor: 39150:forward scatter\|Experimental Factor: 68:side scatter\|Experimental Factor: 480:GFP fluorescence	RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	ERP015799	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2018 11 16	LCK_8_9_1.fq.gz LCK_8_9_2.fq.gz	fastq fastq	321790750.0	1287163.0	E MTAB 46171473686057:LCK 8 9	0:125 1:125	A:92303566;C:70926100;G:65388744;T:92407267;N:765073	125	125	92303566	70926100	65388744	92407267	765073	ERX1690867	ERS1343555	ERA701304	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	2	0.76909	0.77068	0.20517	0.20995	0.97761	0.97822	0.57192	0.56239	125	125	B	B	biological fallback assumption	illumina	hiseq_era	full_length	random_priming	unknown	sc	single_cell_plate	smartseq	Switzerland	2016-05-26	Adult	Adult	Spleen	Hematopoietic System
4254	4254	ERR1620501	ERX1690866	ERS1343554	ERP015799	PRJEB14175	Single cell RNA sequencing of spleen derived LCK cells from adult Zebrafish	E-MTAB-4617	Transcriptome Analysis	Transcriptome data from individual lck:GFP expressing cells isolated from adult Zebrafish spleen. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T cells and NK like and a minor one of myeloid like cells. Single cell transcriptomes are matched with FACS index sorting data GFP forward and side light scatter and dead cell staining	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2016 05 26\|ArrayExpress:E MTAB 4617	Protocols: Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	LCK 8#8	SAMEA4432105	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics	ENA first public:2016 12 01\|ENA last update:2016 09 12\|External Id:SAMEA4432105\|INSDC center alias:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC center name:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC first public:2016 12 01T17:01:25Z\|INSDC last update:2016 09 12T14:14:53Z\|INSDC status:public\|PI fluorescence:9\|Submitter Id:E MTAB 46171473686057:LCK 8#8\|age:3\|broker name:ArrayExpress\|common name:zebrafish\|developmental stage:adult\|forward scatter:38690\|genotype:heterozygous Tglck:EGFP\|gfp fluorescence:719\|individual:2\|organism part:spleen\|plate:8\|sample name:E MTAB 46171473686057:LCK 8#8\|side scatter:35\|single cell well quality:good quality\|well:A8\|well information:single cell	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	E MTAB 46171473686057:LCK 8#8 p	LCK 8#8 p	Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	Experimental Factor: spleen:organism part\|Experimental Factor: 38690:forward scatter\|Experimental Factor: 35:side scatter\|Experimental Factor: 719:GFP fluorescence	RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	ERP015799	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2018 11 16	LCK_8_8_1.fq.gz LCK_8_8_2.fq.gz	fastq fastq	264057500.0	1056230.0	E MTAB 46171473686057:LCK 8 8	0:125 1:125	A:74266704;C:58957248;G:55164989;T:75024394;N:644165	125	125	74266704	58957248	55164989	75024394	644165	ERX1690866	ERS1343554	ERA701304	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	2	0.68044	0.68052	0.1982	0.20001	0.98453	0.98551	0.488	0.58804	125	125	B	B	biological fallback assumption	illumina	hiseq_era	full_length	random_priming	unknown	sc	single_cell_plate	smartseq	Switzerland	2016-05-26	Adult	Adult	Spleen	Hematopoietic System
4255	4255	ERR1620500	ERX1690865	ERS1343553	ERP015799	PRJEB14175	Single cell RNA sequencing of spleen derived LCK cells from adult Zebrafish	E-MTAB-4617	Transcriptome Analysis	Transcriptome data from individual lck:GFP expressing cells isolated from adult Zebrafish spleen. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T cells and NK like and a minor one of myeloid like cells. Single cell transcriptomes are matched with FACS index sorting data GFP forward and side light scatter and dead cell staining	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2016 05 26\|ArrayExpress:E MTAB 4617	Protocols: Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	LCK 8#7	SAMEA4432104	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics	ENA first public:2016 12 01\|ENA last update:2016 09 12\|External Id:SAMEA4432104\|INSDC center alias:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC center name:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC first public:2016 12 01T17:01:25Z\|INSDC last update:2016 09 12T14:14:53Z\|INSDC status:public\|PI fluorescence:44\|Submitter Id:E MTAB 46171473686057:LCK 8#7\|age:3\|broker name:ArrayExpress\|common name:zebrafish\|developmental stage:adult\|forward scatter:30221\|genotype:heterozygous Tglck:EGFP\|gfp fluorescence:845\|individual:2\|organism part:spleen\|plate:8\|sample name:E MTAB 46171473686057:LCK 8#7\|side scatter:29\|single cell well quality:good quality\|well:A7\|well information:single cell	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	E MTAB 46171473686057:LCK 8#7 p	LCK 8#7 p	Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	Experimental Factor: spleen:organism part\|Experimental Factor: 30221:forward scatter\|Experimental Factor: 29:side scatter\|Experimental Factor: 845:GFP fluorescence	RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	ERP015799	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2018 11 16	LCK_8_7_1.fq LCK_8_7_2.fq	fastq fastq	72607000.0	290428.0	E MTAB 46171473686057:LCK 8 7	0:125 1:125	A:20962383;C:16075445;G:14106982;T:21286579;N:175611	125	125	20962383	16075445	14106982	21286579	175611	ERX1690865	ERS1343553	ERA701304	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	2	0.56374	0.55869	0.15373	0.15614	0.98573	0.98748	0.61041	0.61515	125	125	B	B	biological fallback assumption	illumina	hiseq_era	full_length	random_priming	unknown	sc	single_cell_plate	smartseq	Switzerland	2016-05-26	Adult	Adult	Spleen	Hematopoietic System
4256	4256	ERR1620499	ERX1690864	ERS1343552	ERP015799	PRJEB14175	Single cell RNA sequencing of spleen derived LCK cells from adult Zebrafish	E-MTAB-4617	Transcriptome Analysis	Transcriptome data from individual lck:GFP expressing cells isolated from adult Zebrafish spleen. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T cells and NK like and a minor one of myeloid like cells. Single cell transcriptomes are matched with FACS index sorting data GFP forward and side light scatter and dead cell staining	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2016 05 26\|ArrayExpress:E MTAB 4617	Protocols: Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	LCK 8#6	SAMEA4432103	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics	ENA first public:2016 12 01\|ENA last update:2016 09 12\|External Id:SAMEA4432103\|INSDC center alias:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC center name:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC first public:2016 12 01T17:01:25Z\|INSDC last update:2016 09 12T14:14:53Z\|INSDC status:public\|PI fluorescence:46\|Submitter Id:E MTAB 46171473686057:LCK 8#6\|age:3\|broker name:ArrayExpress\|common name:zebrafish\|developmental stage:adult\|forward scatter:33571\|genotype:heterozygous Tglck:EGFP\|gfp fluorescence:661\|individual:2\|organism part:spleen\|plate:8\|sample name:E MTAB 46171473686057:LCK 8#6\|side scatter:54\|single cell well quality:good quality\|well:A6\|well information:single cell	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	E MTAB 46171473686057:LCK 8#6 p	LCK 8#6 p	Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	Experimental Factor: spleen:organism part\|Experimental Factor: 33571:forward scatter\|Experimental Factor: 54:side scatter\|Experimental Factor: 661:GFP fluorescence	RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	ERP015799	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2019 01 23	LCK_8_6_1.fq LCK_8_6_2.fq	fastq fastq	132079500.0	528318.0	E MTAB 46171473686057:LCK 8 6	0:125 1:125	A:37881943;C:29097279;G:26662941;T:38123649;N:313688	125	125	37881943	29097279	26662941	38123649	313688	ERX1690864	ERS1343552	ERA701304	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	2	0.63313	0.62967	0.22695	0.22942	0.98638	0.98774	0.55539	0.55001	125	125	B	B	biological fallback assumption	illumina	hiseq_era	full_length	random_priming	unknown	sc	single_cell_plate	smartseq	Switzerland	2016-05-26	Adult	Adult	Spleen	Hematopoietic System
4257	4257	ERR1620498	ERX1690863	ERS1343551	ERP015799	PRJEB14175	Single cell RNA sequencing of spleen derived LCK cells from adult Zebrafish	E-MTAB-4617	Transcriptome Analysis	Transcriptome data from individual lck:GFP expressing cells isolated from adult Zebrafish spleen. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T cells and NK like and a minor one of myeloid like cells. Single cell transcriptomes are matched with FACS index sorting data GFP forward and side light scatter and dead cell staining	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2016 05 26\|ArrayExpress:E MTAB 4617	Protocols: Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	LCK 8#5	SAMEA4432102	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics	ENA first public:2016 12 01\|ENA last update:2016 09 12\|External Id:SAMEA4432102\|INSDC center alias:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC center name:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC first public:2016 12 01T17:01:25Z\|INSDC last update:2016 09 12T14:14:53Z\|INSDC status:public\|PI fluorescence:55\|Submitter Id:E MTAB 46171473686057:LCK 8#5\|age:3\|broker name:ArrayExpress\|common name:zebrafish\|developmental stage:adult\|forward scatter:38889\|genotype:heterozygous Tglck:EGFP\|gfp fluorescence:2449\|individual:2\|organism part:spleen\|plate:8\|sample name:E MTAB 46171473686057:LCK 8#5\|side scatter:43\|single cell well quality:good quality\|well:A5\|well information:single cell	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	E MTAB 46171473686057:LCK 8#5 p	LCK 8#5 p	Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	Experimental Factor: spleen:organism part\|Experimental Factor: 38889:forward scatter\|Experimental Factor: 43:side scatter\|Experimental Factor: 2449:GFP fluorescence	RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	ERP015799	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2019 01 23	LCK_8_5_1.fq LCK_8_5_2.fq	fastq fastq	259622250.0	1038489.0	E MTAB 46171473686057:LCK 8 5	0:125 1:125	A:70294454;C:60562889;G:55907864;T:72261072;N:595971	125	125	70294454	60562889	55907864	72261072	595971	ERX1690863	ERS1343551	ERA701304	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	2	0.50845	0.50406	0.15833	0.16088	0.97804	0.97944	0.5751	0.57925	125	125	B	B	biological fallback assumption	illumina	hiseq_era	full_length	random_priming	unknown	sc	single_cell_plate	smartseq	Switzerland	2016-05-26	Adult	Adult	Spleen	Hematopoietic System
4258	4258	ERR1620497	ERX1690862	ERS1343550	ERP015799	PRJEB14175	Single cell RNA sequencing of spleen derived LCK cells from adult Zebrafish	E-MTAB-4617	Transcriptome Analysis	Transcriptome data from individual lck:GFP expressing cells isolated from adult Zebrafish spleen. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T cells and NK like and a minor one of myeloid like cells. Single cell transcriptomes are matched with FACS index sorting data GFP forward and side light scatter and dead cell staining	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2016 05 26\|ArrayExpress:E MTAB 4617	Protocols: Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	LCK 8#4	SAMEA4432101	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics	ENA first public:2016 12 01\|ENA last update:2016 09 12\|External Id:SAMEA4432101\|INSDC center alias:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC center name:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC first public:2016 12 01T17:01:25Z\|INSDC last update:2016 09 12T14:14:53Z\|INSDC status:public\|PI fluorescence:44\|Submitter Id:E MTAB 46171473686057:LCK 8#4\|age:3\|broker name:ArrayExpress\|common name:zebrafish\|developmental stage:adult\|forward scatter:34206\|genotype:heterozygous Tglck:EGFP\|gfp fluorescence:609\|individual:2\|organism part:spleen\|plate:8\|sample name:E MTAB 46171473686057:LCK 8#4\|side scatter:29\|single cell well quality:good quality\|well:A4\|well information:single cell	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	E MTAB 46171473686057:LCK 8#4 p	LCK 8#4 p	Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	Experimental Factor: spleen:organism part\|Experimental Factor: 34206:forward scatter\|Experimental Factor: 29:side scatter\|Experimental Factor: 609:GFP fluorescence	RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	ERP015799	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2019 01 23	LCK_8_4_1.fq LCK_8_4_2.fq	fastq fastq	223037500.0	892150.0	E MTAB 46171473686057:LCK 8 4	0:125 1:125	A:64103523;C:48835067;G:44820926;T:64749033;N:528951	125	125	64103523	48835067	44820926	64749033	528951	ERX1690862	ERS1343550	ERA701304	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	2	0.66738	0.66751	0.26433	0.26787	0.98691	0.9879	0.59795	0.6032	125	125	B	B	biological fallback assumption	illumina	hiseq_era	full_length	random_priming	unknown	sc	single_cell_plate	smartseq	Switzerland	2016-05-26	Adult	Adult	Spleen	Hematopoietic System
4259	4259	ERR1620496	ERX1690861	ERS1343549	ERP015799	PRJEB14175	Single cell RNA sequencing of spleen derived LCK cells from adult Zebrafish	E-MTAB-4617	Transcriptome Analysis	Transcriptome data from individual lck:GFP expressing cells isolated from adult Zebrafish spleen. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T cells and NK like and a minor one of myeloid like cells. Single cell transcriptomes are matched with FACS index sorting data GFP forward and side light scatter and dead cell staining	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2016 05 26\|ArrayExpress:E MTAB 4617	Protocols: Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	LCK 8#3	SAMEA4432100	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics	ENA first public:2016 12 01\|ENA last update:2016 09 12\|External Id:SAMEA4432100\|INSDC center alias:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC center name:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC first public:2016 12 01T17:01:25Z\|INSDC last update:2016 09 12T14:14:53Z\|INSDC status:public\|PI fluorescence:15\|Submitter Id:E MTAB 46171473686057:LCK 8#3\|age:3\|broker name:ArrayExpress\|common name:zebrafish\|developmental stage:adult\|forward scatter:39434\|genotype:heterozygous Tglck:EGFP\|gfp fluorescence:1769\|individual:2\|organism part:spleen\|plate:8\|sample name:E MTAB 46171473686057:LCK 8#3\|side scatter:35\|single cell well quality:good quality\|well:A3\|well information:single cell	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	E MTAB 46171473686057:LCK 8#3 p	LCK 8#3 p	Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	Experimental Factor: spleen:organism part\|Experimental Factor: 39434:forward scatter\|Experimental Factor: 35:side scatter\|Experimental Factor: 1769:GFP fluorescence	RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	ERP015799	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2018 11 16	LCK_8_3_1.fq LCK_8_3_2.fq	fastq fastq	80979500.0	323918.0	E MTAB 46171473686057:LCK 8 3	0:125 1:125	A:24733446;C:16721540;G:14416910;T:24901839;N:205765	125	125	24733446	16721540	14416910	24901839	205765	ERX1690861	ERS1343549	ERA701304	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	2	0.65177	0.64614	0.16794	0.16959	0.98336	0.98549	0.55341	0.55637	125	125	B	B	biological fallback assumption	illumina	hiseq_era	full_length	random_priming	unknown	sc	single_cell_plate	smartseq	Switzerland	2016-05-26	Adult	Adult	Spleen	Hematopoietic System
4260	4260	ERR1620495	ERX1690860	ERS1343548	ERP015799	PRJEB14175	Single cell RNA sequencing of spleen derived LCK cells from adult Zebrafish	E-MTAB-4617	Transcriptome Analysis	Transcriptome data from individual lck:GFP expressing cells isolated from adult Zebrafish spleen. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T cells and NK like and a minor one of myeloid like cells. Single cell transcriptomes are matched with FACS index sorting data GFP forward and side light scatter and dead cell staining	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2016 05 26\|ArrayExpress:E MTAB 4617	Protocols: Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	LCK 8#27	SAMEA4432099	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics	ENA first public:2016 12 01\|ENA last update:2016 09 12\|External Id:SAMEA4432099\|INSDC center alias:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC center name:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC first public:2016 12 01T17:01:25Z\|INSDC last update:2016 09 12T14:14:53Z\|INSDC status:public\|PI fluorescence:14\|Submitter Id:E MTAB 46171473686057:LCK 8#27\|age:3\|broker name:ArrayExpress\|common name:zebrafish\|developmental stage:adult\|forward scatter:30818\|genotype:heterozygous Tglck:EGFP\|gfp fluorescence:692\|individual:2\|organism part:spleen\|plate:8\|sample name:E MTAB 46171473686057:LCK 8#27\|side scatter:40\|single cell well quality:good quality\|well:C3\|well information:single cell	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	E MTAB 46171473686057:LCK 8#27 p	LCK 8#27 p	Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	Experimental Factor: spleen:organism part\|Experimental Factor: 30818:forward scatter\|Experimental Factor: 40:side scatter\|Experimental Factor: 692:GFP fluorescence	RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	ERP015799	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2018 11 16	LCK_8_27_1.fq LCK_8_27_2.fq	fastq fastq	1152812500.0	4611250.0	E MTAB 46171473686057:LCK 8 27	0:125 1:125	A:340109783;C:242696953;G:211779212;T:355709569;N:2516983	125	125	340109783	242696953	211779212	355709569	2516983	ERX1690860	ERS1343548	ERA701304	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	2	0.8102	0.81382	0.26627	0.27235	0.9861	0.98656	0.5797	0.5765	125	125	B	B	biological fallback assumption	illumina	hiseq_era	full_length	random_priming	unknown	sc	single_cell_plate	smartseq	Switzerland	2016-05-26	Adult	Adult	Spleen	Hematopoietic System
4261	4261	ERR1620494	ERX1690859	ERS1343547	ERP015799	PRJEB14175	Single cell RNA sequencing of spleen derived LCK cells from adult Zebrafish	E-MTAB-4617	Transcriptome Analysis	Transcriptome data from individual lck:GFP expressing cells isolated from adult Zebrafish spleen. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T cells and NK like and a minor one of myeloid like cells. Single cell transcriptomes are matched with FACS index sorting data GFP forward and side light scatter and dead cell staining	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2016 05 26\|ArrayExpress:E MTAB 4617	Protocols: Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	LCK 8#26	SAMEA4432098	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics	ENA first public:2016 12 01\|ENA last update:2016 09 12\|External Id:SAMEA4432098\|INSDC center alias:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC center name:Ludwig Center for Cancer Research University of Lausanne Lausanne Switzerland Swiss Institute of Bioinformatics\|INSDC first public:2016 12 01T17:01:25Z\|INSDC last update:2016 09 12T14:14:53Z\|INSDC status:public\|PI fluorescence:28\|Submitter Id:E MTAB 46171473686057:LCK 8#26\|age:3\|broker name:ArrayExpress\|common name:zebrafish\|developmental stage:adult\|forward scatter:35854\|genotype:heterozygous Tglck:EGFP\|gfp fluorescence:1048\|individual:2\|organism part:spleen\|plate:8\|sample name:E MTAB 46171473686057:LCK 8#26\|side scatter:64\|single cell well quality:good quality\|well:C2\|well information:single cell	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	E MTAB 46171473686057:LCK 8#26 p	LCK 8#26 p	Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	Spleens from two heterozygous Tglck:EGFP fish were dissected and passed through a 40μm cell strainer using the plunger of a 1 mL syringe and cells were collected in cold 1xPBS/5% FBS. A non transgenic line was used to set up the gating and exclude autofluorescent cells. Propidium iodide PI staining was used to exclude dead cells. Individual cells were sorted using a Becton Dickinson Influx sorter with 488 and 561 nm lasers Schulte et al. 2015 and collected in a single well of a 96 well plate containing 2.3 uL of 0.2 % Triton X 100 supplemented with 1 U/uL SUPERase In RNAse inhibitor Ambion. The size granularity and level of fluorescence for each cell were simultaneously recorded. The Smart seq2 protocol Picelli et al. 2014 was used to amplify the whole transcriptome and prepare libraries. Twenty five cycles of PCR amplification were performed.	Experimental Factor: spleen:organism part\|Experimental Factor: 35854:forward scatter\|Experimental Factor: 64:side scatter\|Experimental Factor: 1048:GFP fluorescence	RNA-Seq	TRANSCRIPTOMIC	cDNA	PAIRED	ILLUMINA	Illumina HiSeq 2000	<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>250</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_LABEL>F</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_LABEL>R</READ_LABEL><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>126</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>	ERP015799	Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing of spleen derived LCK expressing cells from adult zebrafish	ENA FIRST PUBLIC:2016 12 01\|ENA LAST UPDATE:2018 11 16	LCK_8_26_1.fq LCK_8_26_2.fq	fastq fastq	410481250.0	1641925.0	E MTAB 46171473686057:LCK 8 26	0:125 1:125	A:115373409;C:91610126;G:82943446;T:119775715;N:778554	125	125	115373409	91610126	82943446	119775715	778554	ERX1690859	ERS1343547	ERA701304	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	Ludwig Center for Cancer Research, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics\|European Nucleotide Archive	2	0.76258	0.76471	0.24418	0.24963	0.98557	0.98616	0.50436	0.51005	125	125	B	B	biological fallback assumption	illumina	hiseq_era	full_length	random_priming	unknown	sc	single_cell_plate	smartseq	Switzerland	2016-05-26	Adult	Adult	Spleen	Hematopoietic System

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CREATE TABLE run_metadata("run.accession" VARCHAR, "experiment.accession" VARCHAR, "sample.accession" VARCHAR, "study.accession" VARCHAR, bioproject VARCHAR, "study.title" VARCHAR, "study.alias" VARCHAR, "study.type" VARCHAR, "study.abstract" VARCHAR, "study.attributes" VARCHAR, "study.PMIDs" VARCHAR, "sample.description" VARCHAR, "sample.title" VARCHAR, "sample.alias" VARCHAR, "sample.centername" VARCHAR, "sample.attributes" VARCHAR, "GEOsample.title" VARCHAR, "GEOsample.dataprocessing" VARCHAR, "GEOsample.source" VARCHAR, "GEOsample.treatmentprotocol" VARCHAR, "GEOsample.extractprotocol" VARCHAR, "GEOsample.growthprotocol" VARCHAR, "GEOsample.characteristics" VARCHAR, "GEOsample.accession" VARCHAR, "experiment.title" VARCHAR, "experiment.alias" VARCHAR, "experiment.library_name" VARCHAR, "experiment.design_description" VARCHAR, "experiment.library_construction_protocol" VARCHAR, "experiment.attributes" VARCHAR, "experiment.library_strategy" VARCHAR, "experiment.library_source" VARCHAR, "experiment.library_selection" VARCHAR, "experiment.library_layout" VARCHAR, "experiment.platform" VARCHAR, "experiment.instrument_model" VARCHAR, "experiment.spot_descriptor" VARCHAR, "experiment.study_ref" VARCHAR, "run.title" VARCHAR, "run.attributes" VARCHAR, "run.filename" VARCHAR, "run.semantic_name" VARCHAR, "run.total_bases" DOUBLE, "run.total_spots" DOUBLE, "run.alias" VARCHAR, "run.read_lengths" VARCHAR, "run.base_counts" VARCHAR, "run.r1_length" BIGINT, "run.r2_length" BIGINT, "run.r3_length" BIGINT, "run.r4_length" BIGINT, "run.Acount" BIGINT, "run.Ccount" BIGINT, "run.Gcount" BIGINT, "run.Tcount" BIGINT, "run.Ncount" BIGINT, "run.experiment" VARCHAR, "run.pool_member" VARCHAR, "submission.accession" VARCHAR, "submission.srasource" VARCHAR, "submission.bioprojectsource" VARCHAR, "seqdetective.n_mates" BIGINT, "seqdetective.mapping_rate.mate1" DOUBLE, "seqdetective.mapping_rate.mate2" DOUBLE, "seqdetective.nofeature_rate.mate1" DOUBLE, "seqdetective.nofeature_rate.mate2" DOUBLE, "seqdetective.sparsity.mate1" DOUBLE, "seqdetective.sparsity.mate2" DOUBLE, "seqdetective.pos_strand_rate.mate1" DOUBLE, "seqdetective.pos_strand_rate.mate2" DOUBLE, "seqdetective.readlen.mate1" BIGINT, "seqdetective.readlen.mate2" BIGINT, "seqdetective.judgement.mate1" VARCHAR, "seqdetective.judgement.mate2" VARCHAR, "seqdetective.judgement.reason" VARCHAR, platform_family VARCHAR, instrument_generation VARCHAR, read_bias VARCHAR, selection_class VARCHAR, prep_kit VARCHAR, sc_or_bulk VARCHAR, tech_class VARCHAR, technology VARCHAR, tech_variant VARCHAR, "submission.bioprojectsource.country" VARCHAR, earliest_date DATE, devstage_curation VARCHAR, devstage_curation_coarse VARCHAR, tissue_curation VARCHAR, tissue_curation_coarse VARCHAR);;
CREATE INDEX idx_run_bioproject ON run_metadata(bioproject);;
CREATE INDEX idx_run_run_accession ON run_metadata("run.accession");;