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17 rows where experiment.library_layout = "PAIRED", experiment.library_selection = "other" and tissue_curation = "Gonad"

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devstage_curation_coarse 2

devstage_curation 2

tissue_curation_coarse 1

tissue_curation 1

  • Gonad · 17 ✖

technology 1

experiment.platform 1

experiment.library_source 1

experiment.library_selection 1

  • other · 17 ✖

experiment.library_layout 1

  • PAIRED · 17 ✖
Link rowid ▼ run.accession experiment.accession sample.accession study.accession bioproject study.title study.alias study.type study.abstract study.attributes study.PMIDs sample.description sample.title sample.alias sample.centername sample.attributes GEOsample.title GEOsample.dataprocessing GEOsample.source GEOsample.treatmentprotocol GEOsample.extractprotocol GEOsample.growthprotocol GEOsample.characteristics GEOsample.accession experiment.title experiment.alias experiment.library_name experiment.design_description experiment.library_construction_protocol experiment.attributes experiment.library_strategy experiment.library_source experiment.library_selection experiment.library_layout experiment.platform experiment.instrument_model experiment.spot_descriptor experiment.study_ref run.title run.attributes run.filename run.semantic_name run.total_bases run.total_spots run.alias run.read_lengths run.base_counts run.r1_length run.r2_length run.r3_length run.r4_length run.Acount run.Ccount run.Gcount run.Tcount run.Ncount run.experiment run.pool_member submission.accession submission.srasource submission.bioprojectsource seqdetective.n_mates seqdetective.mapping_rate.mate1 seqdetective.mapping_rate.mate2 seqdetective.nofeature_rate.mate1 seqdetective.nofeature_rate.mate2 seqdetective.sparsity.mate1 seqdetective.sparsity.mate2 seqdetective.pos_strand_rate.mate1 seqdetective.pos_strand_rate.mate2 seqdetective.readlen.mate1 seqdetective.readlen.mate2 seqdetective.judgement.mate1 seqdetective.judgement.mate2 seqdetective.judgement.reason platform_family instrument_generation read_bias selection_class prep_kit sc_or_bulk tech_class technology tech_variant submission.bioprojectsource.country earliest_date devstage_curation devstage_curation_coarse tissue_curation tissue_curation_coarse
7949 7949 ERR015566 ERX005931 ERS000090 ERP000263 PRJEB2208 Zebrafish gene three prime end pull down for genome annotation E-MTAB-308 Transcriptome Analysis         ZF ovary sample1 SAMEA708832 Wellcome Sanger Institute Alias:ZF ovary sample1|Description:RNA extracted from adult zebrafish ovary|INSDC center alias:SC|INSDC center name:Wellcome Sanger Institute|INSDC first public:2010 02 26T10:44:13Z|INSDC last update:2018 03 08T15:24:37Z|INSDC status:public|SRA accession:ERS000090|Sample Name:ERS000090|Sex:female|Strain:Tuebingen|Title:Danio rerio                 Illumina Genome Analyzer II paired end sequencing; Zebrafish gene 3 prime end pull down for genome annotation E MTAB 308:Illumina Genome Analyzer II sequencing of adult Zebrafish ovary dpf three prime pull down paired end 250 to 300 bp insert Zebrafish adult ovary mRNA three prime end Zebrafish gene three prime end pull down for genome annotation 20 ug of total RNA was fragmented using RNA Fragmentation Reagent Ambion for 5 minutes at 70 C and ethanol precipitated with glycogen and LiCl. RNA was annealed to the oligo stBPM1polyT22 biotin GGCCAGTCCTGGAGTTTTTTTTTTTTTTTTTTTTTTVN and bound to streptavidin magnetic beads. post washing by pull down on a magnet the bound RNA was reverse transcribed with SuperScript II Invitrogen and a second strand synthesised with DNA polymerase I Promega and RNase H NEB. post further washing the double strand cDNA was released from the beads with BpmI NEB. The cDNA was recovered with the QIAgen PCR Purification Kit and made into a standard Illumina library following the manufacturer's protocol with a fragment size of 250 to 300 bp. Experimental Factor: DEVELOPMENTAL STAGE:adult|Experimental Factor: ORGANISM PART:ovary|Experimental Factor: SEX:female RNA-Seq TRANSCRIPTOMIC other PAIRED ILLUMINA Illumina Genome Analyzer II   ERP000263 Illumina Genome Analyzer II paired end sequencing; Zebrafish gene three prime end pull down for genome annotation ENA FIRST PUBLIC:2010 08 19|ENA LAST UPDATE:2018 11 16 3444_6.srf srf 1150193272.0 7567061.0 E MTAB 308:Illumina Genome Analyzer II sequencing of adult Zebrafish ovary dpf three prime pull down paired end 250 to 300 bp insert 0:76 1:76 A:280621121;C:291623460;G:285422205;T:273609496;N:18916990 76 76     280621121 291623460 285422205 273609496 18916990 ERX005931 ERS000090 ERA010603 SC|Wellcome Trust Sanger Institute SC|Wellcome Trust Sanger Institute 2 0.96663 0.96605 0.0126 0.01249 0.82272 0.82548 0.45602 0.45286 76 76 B B biological fallback assumption illumina early_illumina 3prime other unknown bulk unknown unknown   United Kingdom 2010-02-26 Adult Adult Gonad Reproductive System
68053 68053 SRR17592787 SRX13761632 SRS11641838 SRP354757 PRJNA796639 Augmentation of progestin signaling rescues testis organization and spermatogenesis in zebrafish with the depletion of androgen signaling PRJNA796639 Other         Tko tko   strain:AB tko|age:6 month|dev stage:adult|sex:male|tissue:gonad|BioSampleModel:Model organism or animal                 adult male gonad tko cyp17a1 / ;ar / ;npgr / gonad     RNA-Seq TRANSCRIPTOMIC other PAIRED ILLUMINA Illumina HiSeq 4000   SRP354757     tko_R1.fastq.gz tko_R2.fastq.gz fastq fastq 6433498200.0 21444994.0 tko R1.fastq.gz 0:150 1:150 A:1734295551;C:1481922056;G:1500397917;T:1716815178;N:67498 150 150     1734295551 1481922056 1500397917 1716815178 67498 SRX13761632 SRS11641838 SRA1356016 Chinese Academy of Sciences|Institute of Hydrology Chinese Academy of Sciences 2 0.92657 0.92706 0.08532 0.08571 0.6225 0.62467 0.48954 0.48644 150 150 B B biological fallback assumption illumina hiseq_era unknown other unknown bulk unknown unknown   China 2022-01-12 Adult Adult Gonad Reproductive System
68054 68054 SRR17592788 SRX13761631 SRS11641837 SRP354757 PRJNA796639 Augmentation of progestin signaling rescues testis organization and spermatogenesis in zebrafish with the depletion of androgen signaling PRJNA796639 Other         Ar apdko   strain:AB apdko|age:6 month|dev stage:adult|sex:male|tissue:gonad|BioSampleModel:Model organism or animal                 adult male gonad apdko ar / ;npgr / gonad     RNA-Seq TRANSCRIPTOMIC other PAIRED ILLUMINA Illumina HiSeq 4000   SRP354757     apdko_R1.fastq.gz apdko_R2.fastq.gz fastq fastq 6641915400.0 22139718.0 apdko R1.fastq.gz 0:150 1:150 A:1791028372;C:1527238557;G:1556140309;T:1767435695;N:72467 150 150     1791028372 1527238557 1556140309 1767435695 72467 SRX13761631 SRS11641837 SRA1356016 Chinese Academy of Sciences|Institute of Hydrology Chinese Academy of Sciences 2 0.92557 0.92557 0.09181 0.09106 0.60229 0.60405 0.49189 0.49194 150 150 B B biological fallback assumption illumina hiseq_era unknown other unknown bulk unknown unknown   China 2022-01-12 Adult Adult Gonad Reproductive System
68055 68055 SRR17592789 SRX13761630 SRS11641836 SRP354757 PRJNA796639 Augmentation of progestin signaling rescues testis organization and spermatogenesis in zebrafish with the depletion of androgen signaling PRJNA796639 Other         Cpdko cpdko   strain:AB cpdko|age:6 month|dev stage:adult|sex:male|tissue:gonad|BioSampleModel:Model organism or animal                 adult male gonad cpdko cyp17a1 / ;npgr / gonad     RNA-Seq TRANSCRIPTOMIC other PAIRED ILLUMINA Illumina HiSeq 4000   SRP354757     cpdko_R1.fastq.gz cpdko_R2.fastq.gz fastq fastq 6099098100.0 20330327.0 cpdko R1.fastq.gz 0:150 1:150 A:1650048704;C:1398765282;G:1417954594;T:1632265804;N:63716 150 150     1650048704 1398765282 1417954594 1632265804 63716 SRX13761630 SRS11641836 SRA1356016 Chinese Academy of Sciences|Institute of Hydrology Chinese Academy of Sciences 2 0.92523 0.92507 0.08964 0.08886 0.63092 0.63238 0.48939 0.48876 150 150 B B biological fallback assumption illumina hiseq_era unknown other unknown bulk unknown unknown   China 2022-01-12 Adult Adult Gonad Reproductive System
68056 68056 SRR17592790 SRX13761629 SRS11641835 SRP354757 PRJNA796639 Augmentation of progestin signaling rescues testis organization and spermatogenesis in zebrafish with the depletion of androgen signaling PRJNA796639 Other         Cadko cadko   strain:AB cadko|age:6 month|dev stage:adult|sex:male|tissue:gonad|BioSampleModel:Model organism or animal                 adult male gonad cadko cyp17a1 / ;ar / gonad     RNA-Seq TRANSCRIPTOMIC other PAIRED ILLUMINA Illumina HiSeq 4000   SRP354757     cadko_R1.fastq.gz cadko_R2.fastq.gz fastq fastq 6438923700.0 21463079.0 cadko R1.fastq.gz 0:150 1:150 A:1742816670;C:1475435736;G:1495816081;T:1724787429;N:67784 150 150     1742816670 1475435736 1495816081 1724787429 67784 SRX13761629 SRS11641835 SRA1356016 Chinese Academy of Sciences|Institute of Hydrology Chinese Academy of Sciences 2 0.92668 0.92686 0.09755 0.09752 0.6244 0.6244 0.48164 0.48301 150 150 B B biological fallback assumption illumina hiseq_era unknown other unknown bulk unknown unknown   China 2022-01-12 Adult Adult Gonad Reproductive System
68057 68057 SRR17592791 SRX13761628 SRS11641834 SRP354757 PRJNA796639 Augmentation of progestin signaling rescues testis organization and spermatogenesis in zebrafish with the depletion of androgen signaling PRJNA796639 Other         Npgr npgr   strain:AB npgr|age:6 month|dev stage:adult|sex:male|tissue:gonad|BioSampleModel:Model organism or animal                 adult male gonad npgr npgr / gonad     RNA-Seq TRANSCRIPTOMIC other PAIRED ILLUMINA Illumina HiSeq 4000   SRP354757     npgr_R1.fastq.gz npgr_R2.fastq.gz fastq fastq 6085577700.0 20285259.0 npgr R1.fastq.gz 0:150 1:150 A:1636849675;C:1404433783;G:1424043443;T:1620187148;N:63651 150 150     1636849675 1404433783 1424043443 1620187148 63651 SRX13761628 SRS11641834 SRA1356016 Chinese Academy of Sciences|Institute of Hydrology Chinese Academy of Sciences 2 0.93185 0.93113 0.08334 0.08306 0.63536 0.6354 0.47709 0.48303 150 150 B B biological fallback assumption illumina hiseq_era unknown other unknown bulk unknown unknown   China 2022-01-12 Adult Adult Gonad Reproductive System
68058 68058 SRR17592792 SRX13761627 SRS11641839 SRP354757 PRJNA796639 Augmentation of progestin signaling rescues testis organization and spermatogenesis in zebrafish with the depletion of androgen signaling PRJNA796639 Other         Ar ar   strain:AB ar|age:6 month|dev stage:adult|sex:male|tissue:gonad|BioSampleModel:Model organism or animal                 adult male gonad ar ar / gonad     RNA-Seq TRANSCRIPTOMIC other PAIRED ILLUMINA Illumina HiSeq 4000   SRP354757     ar_R1.fastq.gz ar_R2.fastq.gz fastq fastq 6301409100.0 21004697.0 ar R1.fastq.gz 0:150 1:150 A:1708779082;C:1441426253;G:1457021310;T:1694116414;N:66041 150 150     1708779082 1441426253 1457021310 1694116414 66041 SRX13761627 SRS11641839 SRA1356016 Chinese Academy of Sciences|Institute of Hydrology Chinese Academy of Sciences 2 0.91854 0.91827 0.0948 0.09519 0.60825 0.60898 0.48564 0.48649 150 150 B B biological fallback assumption illumina hiseq_era unknown other unknown bulk unknown unknown   China 2022-01-12 Adult Adult Gonad Reproductive System
68059 68059 SRR17592793 SRX13761626 SRS11641833 SRP354757 PRJNA796639 Augmentation of progestin signaling rescues testis organization and spermatogenesis in zebrafish with the depletion of androgen signaling PRJNA796639 Other         Cyp17a cyp17a   strain:AB Cyp17a|age:6 month|dev stage:adult|sex:male|tissue:gonad|BioSampleModel:Model organism or animal                 adult male gonad cyp17a cyp17a1 / gonad     RNA-Seq TRANSCRIPTOMIC other PAIRED ILLUMINA Illumina HiSeq 4000   SRP354757     cyp17a_R1.fastq.gz cyp17a_R2.fastq.gz fastq fastq 6352758900.0 21175863.0 cyp17a R1.fastq.gz 0:150 1:150 A:1715535098;C:1458777520;G:1479671763;T:1698708117;N:66402 150 150     1715535098 1458777520 1479671763 1698708117 66402 SRX13761626 SRS11641833 SRA1356016 Chinese Academy of Sciences|Institute of Hydrology Chinese Academy of Sciences 2 0.92976 0.92823 0.0895 0.08895 0.63078 0.63236 0.49051 0.48397 150 150 B B biological fallback assumption illumina hiseq_era unknown other unknown bulk unknown unknown   China 2022-01-12 Adult Adult Gonad Reproductive System
68060 68060 SRR17592794 SRX13761625 SRS11641832 SRP354757 PRJNA796639 Augmentation of progestin signaling rescues testis organization and spermatogenesis in zebrafish with the depletion of androgen signaling PRJNA796639 Other         Con con   strain:AB WT|age:6 month|dev stage:adult|sex:male|tissue:gonad|BioSampleModel:Model organism or animal                 adult male gonad con control gonad     RNA-Seq TRANSCRIPTOMIC other PAIRED ILLUMINA Illumina HiSeq 4000   SRP354757     con_R1.fastq.gz con_R2.fastq.gz fastq fastq 6451282800.0 21504276.0 con R1.fastq.gz 0:150 1:150 A:1739038433;C:1485578155;G:1502949152;T:1723649684;N:67376 150 150     1739038433 1485578155 1502949152 1723649684 67376 SRX13761625 SRS11641832 SRA1356016 Chinese Academy of Sciences|Institute of Hydrology Chinese Academy of Sciences 2 0.92909 0.92982 0.08557 0.08607 0.63453 0.637 0.48821 0.48843 150 150 B B biological fallback assumption illumina hiseq_era unknown other unknown bulk unknown unknown   China 2022-01-12 Adult Adult Gonad Reproductive System
68556 68556 SRR18028171 SRX14182301 SRS12005942 SRP359829 PRJNA807002 samll RNA seq of zebrafish gonads of wildtype and pld6 / at 22 dpf PRJNA807002 Other In this study we generated zebrafish pld6 knockouts and found that the pld6 null mutants developed as infertile males with testes containing no germ cells. Pld6 is considered to be a candidate ribonuclease participating in primary piRNA biogenesis which is essential for the survival of germ cells. We deep sequenced 20 33 nt total small RNAs obtained from 22 dpf wildtype and pld6 / gonad with three repetition. In pld6 / gonad small RNAs mapped to the known piRNA clusters were almost absent. Moreover piRNA clusters in pld6 / gonad lost the enrichment for uridine at the first position or for adenine at the 10th position. our results unequivocally demonstrate a conserved role for MitoPLD in the piRNA biogenesis pathway.         6WT 3   strain:AB|isolate:replicate3|ecotype:wt|age:post fertilization day 22|dev stage:juvenile|sex:missing|tissue:Gonad|BioSampleModel:Model organism or animal                 wt 3 S11 L001 R1 011 011 Zebrafish larvae were anesthetized in 0.2 mg/mL MS 222 and disected to isolate gonads under a microscope. Three wildtype or mutant gonads were mixed into one repeat and three repeats were performed for samll RNA sequencing.     ncRNA-Seq TRANSCRIPTOMIC other PAIRED ILLUMINA HiSeq X Ten   SRP359829     wt-3_S11_L001_R1_001.fastq.gz wt-3_S11_L001_R2_001.fastq.gz fastq fastq 3576981600.0 11923272.0 wt 3 S11 L001 R1 001.fastq.gz 0:150 1:150 A:689001630;C:496844981;G:1779990511;T:610974133;N:170345 150 150     689001630 496844981 1779990511 610974133 170345 SRX14182301 SRS12005942 SRA1372890 Huazhong agricultural university|School of Life Science and Technology Huazhong agricultural university 2 0.8179 0.81697 0.60905 0.60864 0.82692 0.82915 0.52425 0.52623 150 150 B B biological fallback assumption illumina hiseq_era unknown small_rna unknown bulk unknown unknown   China 2022-02-14 Multi-stage Multi-stage Gonad Reproductive System
68557 68557 SRR18028172 SRX14182300 SRS12005941 SRP359829 PRJNA807002 samll RNA seq of zebrafish gonads of wildtype and pld6 / at 22 dpf PRJNA807002 Other In this study we generated zebrafish pld6 knockouts and found that the pld6 null mutants developed as infertile males with testes containing no germ cells. Pld6 is considered to be a candidate ribonuclease participating in primary piRNA biogenesis which is essential for the survival of germ cells. We deep sequenced 20 33 nt total small RNAs obtained from 22 dpf wildtype and pld6 / gonad with three repetition. In pld6 / gonad small RNAs mapped to the known piRNA clusters were almost absent. Moreover piRNA clusters in pld6 / gonad lost the enrichment for uridine at the first position or for adenine at the 10th position. our results unequivocally demonstrate a conserved role for MitoPLD in the piRNA biogenesis pathway.         5WT 2   strain:AB|isolate:replicate2|ecotype:wt|age:post fertilization day 22|dev stage:juvenile|sex:missing|tissue:Gonad|BioSampleModel:Model organism or animal                 wt 2 S10 L001 R1 009 009 Zebrafish larvae were anesthetized in 0.2 mg/mL MS 222 and disected to isolate gonads under a microscope. Three wildtype or mutant gonads were mixed into one repeat and three repeats were performed for samll RNA sequencing.     ncRNA-Seq TRANSCRIPTOMIC other PAIRED ILLUMINA HiSeq X Ten   SRP359829     wt-2_S10_L001_R1_001.fastq.gz wt-2_S10_L001_R2_001.fastq.gz fastq fastq 4200013800.0 14000046.0 wt 2 S10 L001 R1 001.fastq.gz 0:150 1:150 A:781057232;C:617020885;G:2110010128;T:691723797;N:201758 150 150     781057232 617020885 2110010128 691723797 201758 SRX14182300 SRS12005941 SRA1372890 Huazhong agricultural university|School of Life Science and Technology Huazhong agricultural university 2 0.8213 0.82216 0.59414 0.59507 0.81089 0.81306 0.53839 0.52755 150 150 B B biological fallback assumption illumina hiseq_era unknown small_rna unknown bulk unknown unknown   China 2022-02-14 Multi-stage Multi-stage Gonad Reproductive System
68558 68558 SRR18028173 SRX14182299 SRS12005940 SRP359829 PRJNA807002 samll RNA seq of zebrafish gonads of wildtype and pld6 / at 22 dpf PRJNA807002 Other In this study we generated zebrafish pld6 knockouts and found that the pld6 null mutants developed as infertile males with testes containing no germ cells. Pld6 is considered to be a candidate ribonuclease participating in primary piRNA biogenesis which is essential for the survival of germ cells. We deep sequenced 20 33 nt total small RNAs obtained from 22 dpf wildtype and pld6 / gonad with three repetition. In pld6 / gonad small RNAs mapped to the known piRNA clusters were almost absent. Moreover piRNA clusters in pld6 / gonad lost the enrichment for uridine at the first position or for adenine at the 10th position. our results unequivocally demonstrate a conserved role for MitoPLD in the piRNA biogenesis pathway.         4WT 1   strain:AB|isolate:replicate1|ecotype:wt|age:post fertilization day 22|dev stage:juvenile|sex:missing|tissue:Gonad|BioSampleModel:Model organism or animal                 wt 1 S9 L001 R1 007 007 Zebrafish larvae were anesthetized in 0.2 mg/mL MS 222 and disected to isolate gonads under a microscope. Three wildtype or mutant gonads were mixed into one repeat and three repeats were performed for samll RNA sequencing.     ncRNA-Seq TRANSCRIPTOMIC other PAIRED ILLUMINA HiSeq X Ten   SRP359829     wt-1_S9_L001_R1_001.fastq.gz wt-1_S9_L001_R2_001.fastq.gz fastq fastq 4820063700.0 16066879.0 wt 1 S9 L001 R1 001.fastq.gz 0:150 1:150 A:996304548;C:657334691;G:2379519106;T:786675131;N:230224 150 150     996304548 657334691 2379519106 786675131 230224 SRX14182299 SRS12005940 SRA1372890 Huazhong agricultural university|School of Life Science and Technology Huazhong agricultural university 2 0.80219 0.80319 0.60906 0.60878 0.83238 0.83303 0.52559 0.51852 150 150 B B biological fallback assumption illumina hiseq_era unknown small_rna unknown bulk unknown unknown   China 2022-02-14 Multi-stage Multi-stage Gonad Reproductive System
68559 68559 SRR18028174 SRX14182298 SRS12005939 SRP359829 PRJNA807002 samll RNA seq of zebrafish gonads of wildtype and pld6 / at 22 dpf PRJNA807002 Other In this study we generated zebrafish pld6 knockouts and found that the pld6 null mutants developed as infertile males with testes containing no germ cells. Pld6 is considered to be a candidate ribonuclease participating in primary piRNA biogenesis which is essential for the survival of germ cells. We deep sequenced 20 33 nt total small RNAs obtained from 22 dpf wildtype and pld6 / gonad with three repetition. In pld6 / gonad small RNAs mapped to the known piRNA clusters were almost absent. Moreover piRNA clusters in pld6 / gonad lost the enrichment for uridine at the first position or for adenine at the 10th position. our results unequivocally demonstrate a conserved role for MitoPLD in the piRNA biogenesis pathway.         3PLD6 3   strain:AB|isolate:replicate3|ecotype:pld6 / |age:post fertilization day 22|dev stage:juvenile|sex:missing|tissue:Gonad|BioSampleModel:Model organism or animal                 pld6 3 S3 L003 R1 005 005 Zebrafish larvae were anesthetized in 0.2 mg/mL MS 222 and disected to isolate gonads under a microscope. Three wildtype or mutant gonads were mixed into one repeat and three repeats were performed for samll RNA sequencing.     ncRNA-Seq TRANSCRIPTOMIC other PAIRED ILLUMINA HiSeq X Ten   SRP359829     pld6-3_S3_L003_R1_001.fastq.gz pld6-3_S3_L003_R2_001.fastq.gz fastq fastq 816686700.0 2722289.0 pld6 3 S3 L003 R1 001.fastq.gz 0:150 1:150 A:145681738;C:137123477;G:401610833;T:132256312;N:14340 150 150     145681738 137123477 401610833 132256312 14340 SRX14182298 SRS12005939 SRA1372890 Huazhong agricultural university|School of Life Science and Technology Huazhong agricultural university 2 0.78314 0.73942 0.07641 0.09097 0.91603 0.91545 0.6312 0.64467 150 150 B B biological fallback assumption illumina hiseq_era unknown small_rna unknown bulk unknown unknown   China 2022-02-14 Multi-stage Multi-stage Gonad Reproductive System
68560 68560 SRR18028175 SRX14182297 SRS12005938 SRP359829 PRJNA807002 samll RNA seq of zebrafish gonads of wildtype and pld6 / at 22 dpf PRJNA807002 Other In this study we generated zebrafish pld6 knockouts and found that the pld6 null mutants developed as infertile males with testes containing no germ cells. Pld6 is considered to be a candidate ribonuclease participating in primary piRNA biogenesis which is essential for the survival of germ cells. We deep sequenced 20 33 nt total small RNAs obtained from 22 dpf wildtype and pld6 / gonad with three repetition. In pld6 / gonad small RNAs mapped to the known piRNA clusters were almost absent. Moreover piRNA clusters in pld6 / gonad lost the enrichment for uridine at the first position or for adenine at the 10th position. our results unequivocally demonstrate a conserved role for MitoPLD in the piRNA biogenesis pathway.         2PLD6 2   strain:AB|isolate:replicate2|ecotype:pld6 / |age:post fertilization day 22|dev stage:juvenile|sex:missing|tissue:Gonad|BioSampleModel:Model organism or animal                 pld6 2 S2 L003 R1 003 003 Zebrafish larvae were anesthetized in 0.2 mg/mL MS 222 and disected to isolate gonads under a microscope. Three wildtype or mutant gonads were mixed into one repeat and three repeats were performed for samll RNA sequencing.     ncRNA-Seq TRANSCRIPTOMIC other PAIRED ILLUMINA HiSeq X Ten   SRP359829     pld6-2_S2_L003_R1_001.fastq.gz pld6-2_S2_L003_R2_001.fastq.gz fastq fastq 6546276900.0 21820923.0 pld6 2 S2 L003 R1 001.fastq.gz 0:150 1:150 A:1161502458;C:1095580994;G:3226725392;T:1062358099;N:109957 150 150     1161502458 1095580994 3226725392 1062358099 109957 SRX14182297 SRS12005938 SRA1372890 Huazhong agricultural university|School of Life Science and Technology Huazhong agricultural university 2 0.6471 0.593 0.07935 0.09168 0.92058 0.92005 0.53626 0.65284 150 150 B B biological fallback assumption illumina hiseq_era unknown small_rna unknown bulk unknown unknown   China 2022-02-14 Multi-stage Multi-stage Gonad Reproductive System
68561 68561 SRR18028176 SRX14182296 SRS12005937 SRP359829 PRJNA807002 samll RNA seq of zebrafish gonads of wildtype and pld6 / at 22 dpf PRJNA807002 Other In this study we generated zebrafish pld6 knockouts and found that the pld6 null mutants developed as infertile males with testes containing no germ cells. Pld6 is considered to be a candidate ribonuclease participating in primary piRNA biogenesis which is essential for the survival of germ cells. We deep sequenced 20 33 nt total small RNAs obtained from 22 dpf wildtype and pld6 / gonad with three repetition. In pld6 / gonad small RNAs mapped to the known piRNA clusters were almost absent. Moreover piRNA clusters in pld6 / gonad lost the enrichment for uridine at the first position or for adenine at the 10th position. our results unequivocally demonstrate a conserved role for MitoPLD in the piRNA biogenesis pathway.         1PLD6 1   strain:AB|isolate:replicate1|ecotype:pld6 / |age:post fertilization day 22|dev stage:juvenile|sex:missing|tissue:Gonad|BioSampleModel:Model organism or animal                 pld6 1 S1 L003 R1 001 001 Zebrafish larvae were anesthetized in 0.2 mg/mL MS 222 and disected to isolate gonads under a microscope. Three wildtype or mutant gonads were mixed into one repeat and three repeats were performed for samll RNA sequencing.     ncRNA-Seq TRANSCRIPTOMIC other PAIRED ILLUMINA HiSeq X Ten   SRP359829     pld6-1_S1_L003_R1_001.fastq.gz pld6-1_S1_L003_R2_001.fastq.gz fastq fastq 5535130200.0 18450434.0 pld6 1 S1 L003 R1 001.fastq.gz 0:150 1:150 A:1014761790;C:833992394;G:2813863824;T:872418512;N:93680 150 150     1014761790 833992394 2813863824 872418512 93680 SRX14182296 SRS12005937 SRA1372890 Huazhong agricultural university|School of Life Science and Technology Huazhong agricultural university 2 0.70358 0.73288 0.08649 0.09366 0.91066 0.90914 0.6197 0.6207 150 150 B B biological fallback assumption illumina hiseq_era unknown small_rna unknown bulk unknown unknown   China 2022-02-14 Multi-stage Multi-stage Gonad Reproductive System
74905 74905 SRR24124281 SRX19923332 SRS17275061 SRP431889 PRJNA954142 Zebrafish Gonad sequencing PRJNA954142 Other RNA sequencing of male and female gonads of zebrafish         Female Zebrafish   strain:wild type|age:90 days|sex:female|tissue:Gonad|BioSampleModel:Model organism or animal                 Femalegonad FCHGTWKCCXY L9 WHZEBjyrEAAFRABPEI 37 1 FCHGTWKCCXY L9 WHZEBjyrEAAFRABPEI 37 1 RNA was extracted from tissues of individuals using TRIzol reagent Invitrogen USA. library prep of each individual using the standard protocol provided by Illumina. 150 bp paired end Illumina reads of transcriptome were sequenced on the HiSeq2500 platform.     RNA-Seq TRANSCRIPTOMIC other PAIRED ILLUMINA Illumina HiSeq 2500   SRP431889   loader:fastq load.py FCHGTWKCCXY_L9_WHZEBjyrEAAFRABPEI-37_1.fq FCHGTWKCCXY_L9_WHZEBjyrEAAFRABPEI-37_2.fq fastq fastq 4237141500.0 14123805.0 FCHGTWKCCXY L9 WHZEBjyrEAAFRABPEI 37 1.fq 0:150 1:150 A:1134013231;C:997301614;G:984327511;T:1121230343;N:268801 150 150     1134013231 997301614 984327511 1121230343 268801 SRX19923332 SRS17275061 SRA1618700 Madras Christian College|Zoology Madras Christian College 2 0.88523 0.88363 0.05083 0.05161 0.80381 0.80809 0.55041 0.52553 150 150 B B biological fallback assumption illumina hiseq_era unknown other unknown bulk unknown unknown   India 2023-04-12 Adult Adult Gonad Reproductive System
74906 74906 SRR24124282 SRX19923331 SRS17275060 SRP431889 PRJNA954142 Zebrafish Gonad sequencing PRJNA954142 Other RNA sequencing of male and female gonads of zebrafish         Male zebrafish   strain:wild type|age:90 days|sex:male|tissue:Gonad|BioSampleModel:Model organism or animal                 Malegonad FCHGTWKCCXY L9 WHZEBjyrEAAARAAPEI 27 1 FCHGTWKCCXY L9 WHZEBjyrEAAARAAPEI 27 1 RNA was extracted from tissues of individuals using TRIzol reagent Invitrogen USA. library prep of each individual using the standard protocol provided by Illumina. 150 bp paired end Illumina reads of transcriptome were sequenced on the HiSeq2500 platform.     RNA-Seq TRANSCRIPTOMIC other PAIRED ILLUMINA Illumina HiSeq 2500   SRP431889   loader:fastq load.py FCHGTWKCCXY_L9_WHZEBjyrEAAARAAPEI-27_1.fq FCHGTWKCCXY_L9_WHZEBjyrEAAARAAPEI-27_2.fq fastq fastq 3103334700.0 10344449.0 FCHGTWKCCXY L9 WHZEBjyrEAAARAAPEI 27 1.fq 0:150 1:150 A:838963909;C:717586934;G:712046035;T:834529752;N:208070 150 150     838963909 717586934 712046035 834529752 208070 SRX19923331 SRS17275060 SRA1618700 Madras Christian College|Zoology Madras Christian College 2 0.91689 0.91731 0.06074 0.06165 0.74481 0.75032 0.53861 0.57817 150 150 B B biological fallback assumption illumina hiseq_era unknown other unknown bulk unknown unknown   India 2023-04-12 Adult Adult Gonad Reproductive System

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CREATE TABLE run_metadata("run.accession" VARCHAR, "experiment.accession" VARCHAR, "sample.accession" VARCHAR, "study.accession" VARCHAR, bioproject VARCHAR, "study.title" VARCHAR, "study.alias" VARCHAR, "study.type" VARCHAR, "study.abstract" VARCHAR, "study.attributes" VARCHAR, "study.PMIDs" VARCHAR, "sample.description" VARCHAR, "sample.title" VARCHAR, "sample.alias" VARCHAR, "sample.centername" VARCHAR, "sample.attributes" VARCHAR, "GEOsample.title" VARCHAR, "GEOsample.dataprocessing" VARCHAR, "GEOsample.source" VARCHAR, "GEOsample.treatmentprotocol" VARCHAR, "GEOsample.extractprotocol" VARCHAR, "GEOsample.growthprotocol" VARCHAR, "GEOsample.characteristics" VARCHAR, "GEOsample.accession" VARCHAR, "experiment.title" VARCHAR, "experiment.alias" VARCHAR, "experiment.library_name" VARCHAR, "experiment.design_description" VARCHAR, "experiment.library_construction_protocol" VARCHAR, "experiment.attributes" VARCHAR, "experiment.library_strategy" VARCHAR, "experiment.library_source" VARCHAR, "experiment.library_selection" VARCHAR, "experiment.library_layout" VARCHAR, "experiment.platform" VARCHAR, "experiment.instrument_model" VARCHAR, "experiment.spot_descriptor" VARCHAR, "experiment.study_ref" VARCHAR, "run.title" VARCHAR, "run.attributes" VARCHAR, "run.filename" VARCHAR, "run.semantic_name" VARCHAR, "run.total_bases" DOUBLE, "run.total_spots" DOUBLE, "run.alias" VARCHAR, "run.read_lengths" VARCHAR, "run.base_counts" VARCHAR, "run.r1_length" BIGINT, "run.r2_length" BIGINT, "run.r3_length" BIGINT, "run.r4_length" BIGINT, "run.Acount" BIGINT, "run.Ccount" BIGINT, "run.Gcount" BIGINT, "run.Tcount" BIGINT, "run.Ncount" BIGINT, "run.experiment" VARCHAR, "run.pool_member" VARCHAR, "submission.accession" VARCHAR, "submission.srasource" VARCHAR, "submission.bioprojectsource" VARCHAR, "seqdetective.n_mates" BIGINT, "seqdetective.mapping_rate.mate1" DOUBLE, "seqdetective.mapping_rate.mate2" DOUBLE, "seqdetective.nofeature_rate.mate1" DOUBLE, "seqdetective.nofeature_rate.mate2" DOUBLE, "seqdetective.sparsity.mate1" DOUBLE, "seqdetective.sparsity.mate2" DOUBLE, "seqdetective.pos_strand_rate.mate1" DOUBLE, "seqdetective.pos_strand_rate.mate2" DOUBLE, "seqdetective.readlen.mate1" BIGINT, "seqdetective.readlen.mate2" BIGINT, "seqdetective.judgement.mate1" VARCHAR, "seqdetective.judgement.mate2" VARCHAR, "seqdetective.judgement.reason" VARCHAR, platform_family VARCHAR, instrument_generation VARCHAR, read_bias VARCHAR, selection_class VARCHAR, prep_kit VARCHAR, sc_or_bulk VARCHAR, tech_class VARCHAR, technology VARCHAR, tech_variant VARCHAR, "submission.bioprojectsource.country" VARCHAR, earliest_date DATE, devstage_curation VARCHAR, devstage_curation_coarse VARCHAR, tissue_curation VARCHAR, tissue_curation_coarse VARCHAR);;
CREATE INDEX idx_run_bioproject ON run_metadata(bioproject);;
CREATE INDEX idx_run_run_accession ON run_metadata("run.accession");;