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run_metadata

97 rows where experiment.library_layout = "PAIRED", experiment.library_selection = "PolyA" and tissue_curation = "Trunk"

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devstage_curation_coarse 3

devstage_curation 3

experiment.platform 2

tissue_curation_coarse 1

tissue_curation 1

  • Trunk · 97 ✖

technology 1

experiment.library_strategy 1

experiment.library_source 1

experiment.library_selection 1

  • PolyA · 97 ✖

experiment.library_layout 1

  • PAIRED · 97 ✖
Link rowid ▼ run.accession experiment.accession sample.accession study.accession bioproject study.title study.alias study.type study.abstract study.attributes study.PMIDs sample.description sample.title sample.alias sample.centername sample.attributes GEOsample.title GEOsample.dataprocessing GEOsample.source GEOsample.treatmentprotocol GEOsample.extractprotocol GEOsample.growthprotocol GEOsample.characteristics GEOsample.accession experiment.title experiment.alias experiment.library_name experiment.design_description experiment.library_construction_protocol experiment.attributes experiment.library_strategy experiment.library_source experiment.library_selection experiment.library_layout experiment.platform experiment.instrument_model experiment.spot_descriptor experiment.study_ref run.title run.attributes run.filename run.semantic_name run.total_bases run.total_spots run.alias run.read_lengths run.base_counts run.r1_length run.r2_length run.r3_length run.r4_length run.Acount run.Ccount run.Gcount run.Tcount run.Ncount run.experiment run.pool_member submission.accession submission.srasource submission.bioprojectsource seqdetective.n_mates seqdetective.mapping_rate.mate1 seqdetective.mapping_rate.mate2 seqdetective.nofeature_rate.mate1 seqdetective.nofeature_rate.mate2 seqdetective.sparsity.mate1 seqdetective.sparsity.mate2 seqdetective.pos_strand_rate.mate1 seqdetective.pos_strand_rate.mate2 seqdetective.readlen.mate1 seqdetective.readlen.mate2 seqdetective.judgement.mate1 seqdetective.judgement.mate2 seqdetective.judgement.reason platform_family instrument_generation read_bias selection_class prep_kit sc_or_bulk tech_class technology tech_variant submission.bioprojectsource.country earliest_date devstage_curation devstage_curation_coarse tissue_curation tissue_curation_coarse
33125 33125 SRR29811637 SRX25310837 SRS21984646 SRP519625 PRJNA1132896 Zebrafish larvae exposed by tire wear particles leachate and latex paticles leachate PRJNA1132896 Other This study focuses on investigating the effects of exposure to tire wear particles leachate and latex particles leachate on zebrafish embryos. Specifically we aim to understand how these common environmental pollutants impact the development and health of aquatic organisms with an emphasis on physiological and developmental changes in zebrafish embryos. Our research provides valuable insights into the potential environmental risks associated with tire and latex particle pollutants contributing to the broader field of ecotoxicology and environmental safety.       long term LAP leachate 3 LLAP3   breed:AB|age:120 hours 9|collection date:2024 04 28|geo loc name:China: Qingdao|sex:not collected|tissue:whole body|BioSampleModel:Model organism or animal                 RNA Seq of danio rerio: whole body of larvae LR240511O 1471A LR240511O 1471A mRNA seq of Danio rerio relication 3 of LLAP     RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina HiSeq 2500   SRP519625     LR3.R2.fq.gz LR3.R1.fq.gz fastq fastq 7498719600.0 24995732.0 LR3.R1.fq.gz 0:150 1:150 A:1840764729;C:1818513353;G:1983502172;T:1855787349;N:151997 150 150     1840764729 1818513353 1983502172 1855787349 151997 SRX25310837 SRS21984646 SRA1918576 Qingdao University of Science and Technology|College of Marine Science and biological engineeri Qingdao University of Science and Technology 2 0.9136 0.89307 0.07212 0.07059 0.6646 0.66661 0.47616 0.47357 150 150 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   China 2024-07-12 Larval Larval Trunk Surface Structure
33126 33126 SRR29811638 SRX25310836 SRS21984651 SRP519625 PRJNA1132896 Zebrafish larvae exposed by tire wear particles leachate and latex paticles leachate PRJNA1132896 Other This study focuses on investigating the effects of exposure to tire wear particles leachate and latex particles leachate on zebrafish embryos. Specifically we aim to understand how these common environmental pollutants impact the development and health of aquatic organisms with an emphasis on physiological and developmental changes in zebrafish embryos. Our research provides valuable insights into the potential environmental risks associated with tire and latex particle pollutants contributing to the broader field of ecotoxicology and environmental safety.       long term LAP leachate 2 LLAP2   breed:AB|age:120 hours 8|collection date:2024 04 28|geo loc name:China: Qingdao|sex:not collected|tissue:whole body|BioSampleModel:Model organism or animal                 RNA Seq of danio rerio: whole body of larvae LR240511O 1470A LR240511O 1470A mRNA seq of Danio rerio relication 2 of LLAP     RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina HiSeq 2500   SRP519625     LR2.R1.fq.gz LR2.R2.fq.gz fastq fastq 6564182700.0 21880609.0 LR2.R1.fq.gz 0:150 1:150 A:1746763337;C:1207489067;G:1880015332;T:1729775508;N:139456 150 150     1746763337 1207489067 1880015332 1729775508 139456 SRX25310836 SRS21984651 SRA1918576 Qingdao University of Science and Technology|College of Marine Science and biological engineeri Qingdao University of Science and Technology 2 0.88206 0.77219 0.15745 0.13819 0.7024 0.70473 0.50824 0.50933 150 150 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   China 2024-07-12 Larval Larval Trunk Surface Structure
33127 33127 SRR29811639 SRX25310835 SRS21984645 SRP519625 PRJNA1132896 Zebrafish larvae exposed by tire wear particles leachate and latex paticles leachate PRJNA1132896 Other This study focuses on investigating the effects of exposure to tire wear particles leachate and latex particles leachate on zebrafish embryos. Specifically we aim to understand how these common environmental pollutants impact the development and health of aquatic organisms with an emphasis on physiological and developmental changes in zebrafish embryos. Our research provides valuable insights into the potential environmental risks associated with tire and latex particle pollutants contributing to the broader field of ecotoxicology and environmental safety.       long term LAP leachate 1 LLAP1   breed:AB|age:120 hours 7|collection date:2024 04 28|geo loc name:China: Qingdao|sex:not collected|tissue:whole body|BioSampleModel:Model organism or animal                 RNA Seq of danio rerio: whole body of larvae LR240511O 1469A LR240511O 1469A mRNA seq of Danio rerio relication 1 of LLAP     RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina HiSeq 2500   SRP519625     LR1.R2.fq.gz LR1.R1.fq.gz fastq fastq 7872238200.0 26240794.0 LR1.R1.fq.gz 0:150 1:150 A:2072343735;C:1788675601;G:1954568005;T:2056488656;N:162203 150 150     2072343735 1788675601 1954568005 2056488656 162203 SRX25310835 SRS21984645 SRA1918576 Qingdao University of Science and Technology|College of Marine Science and biological engineeri Qingdao University of Science and Technology 2 0.89866 0.88369 0.11023 0.10907 0.67491 0.67683 0.4824 0.48524 150 150 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   China 2024-07-12 Larval Larval Trunk Surface Structure
33128 33128 SRR29811640 SRX25310834 SRS21984644 SRP519625 PRJNA1132896 Zebrafish larvae exposed by tire wear particles leachate and latex paticles leachate PRJNA1132896 Other This study focuses on investigating the effects of exposure to tire wear particles leachate and latex particles leachate on zebrafish embryos. Specifically we aim to understand how these common environmental pollutants impact the development and health of aquatic organisms with an emphasis on physiological and developmental changes in zebrafish embryos. Our research provides valuable insights into the potential environmental risks associated with tire and latex particle pollutants contributing to the broader field of ecotoxicology and environmental safety.       long term TWP leachate 3 LTWP3   breed:AB|age:120 hours 6|collection date:2024 04 28|geo loc name:China: Qingdao|sex:not collected|tissue:whole body|BioSampleModel:Model organism or animal                 RNA Seq of danio rerio: whole body of larvae LR240511O 1468A LR240511O 1468A mRNA seq of Danio rerio relication 3 of LTWP     RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina HiSeq 2500   SRP519625     MR3.R1.fq.gz MR3.R2.fq.gz fastq fastq 6425482800.0 21418276.0 MR3.R1.fq.gz 0:150 1:150 A:1590904460;C:1556914666;G:1685823262;T:1591710906;N:129506 150 150     1590904460 1556914666 1685823262 1591710906 129506 SRX25310834 SRS21984644 SRA1918576 Qingdao University of Science and Technology|College of Marine Science and biological engineeri Qingdao University of Science and Technology 2 0.91895 0.90759 0.07582 0.07534 0.67446 0.67485 0.46453 0.46627 150 150 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   China 2024-07-12 Larval Larval Trunk Surface Structure
33129 33129 SRR29811641 SRX25310833 SRS21984643 SRP519625 PRJNA1132896 Zebrafish larvae exposed by tire wear particles leachate and latex paticles leachate PRJNA1132896 Other This study focuses on investigating the effects of exposure to tire wear particles leachate and latex particles leachate on zebrafish embryos. Specifically we aim to understand how these common environmental pollutants impact the development and health of aquatic organisms with an emphasis on physiological and developmental changes in zebrafish embryos. Our research provides valuable insights into the potential environmental risks associated with tire and latex particle pollutants contributing to the broader field of ecotoxicology and environmental safety.       long term TWP leachate 2 LTWP2   breed:AB|age:120 hours 5|collection date:2024 04 28|geo loc name:China: Qingdao|sex:not collected|tissue:whole body|BioSampleModel:Model organism or animal                 RNA Seq of danio rerio: whole body of larvae LR240511O 1467A LR240511O 1467A mRNA seq of Danio rerio relication 2 of LTWP     RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina HiSeq 2500   SRP519625     MR2.R1.fq.gz MR2.R2.fq.gz fastq fastq 6383533200.0 21278444.0 MR2.R1.fq.gz 0:150 1:150 A:1717910384;C:1380510195;G:1592327928;T:1692660754;N:123939 150 150     1717910384 1380510195 1592327928 1692660754 123939 SRX25310833 SRS21984643 SRA1918576 Qingdao University of Science and Technology|College of Marine Science and biological engineeri Qingdao University of Science and Technology 2 0.88766 0.86214 0.12652 0.12334 0.68631 0.68952 0.48978 0.48943 150 150 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   China 2024-07-12 Larval Larval Trunk Surface Structure
33130 33130 SRR29811642 SRX25310832 SRS21984642 SRP519625 PRJNA1132896 Zebrafish larvae exposed by tire wear particles leachate and latex paticles leachate PRJNA1132896 Other This study focuses on investigating the effects of exposure to tire wear particles leachate and latex particles leachate on zebrafish embryos. Specifically we aim to understand how these common environmental pollutants impact the development and health of aquatic organisms with an emphasis on physiological and developmental changes in zebrafish embryos. Our research provides valuable insights into the potential environmental risks associated with tire and latex particle pollutants contributing to the broader field of ecotoxicology and environmental safety.       long term TWP leachate 1 LTWP1   breed:AB|age:120 hours 4|collection date:2024 04 28|geo loc name:China: Qingdao|sex:not collected|tissue:whole body|BioSampleModel:Model organism or animal                 RNA Seq of danio rerio: whole body of larvae LR240511O 1466A LR240511O 1466A mRNA seq of Danio rerio relication 1 of LTWP     RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina HiSeq 2500   SRP519625     MR1.R1.fq.gz MR1.R2.fq.gz fastq fastq 7846995900.0 26156653.0 MR1.R1.fq.gz 0:150 1:150 A:1992383226;C:1863588260;G:1991793371;T:1999072963;N:158080 150 150     1992383226 1863588260 1991793371 1999072963 158080 SRX25310832 SRS21984642 SRA1918576 Qingdao University of Science and Technology|College of Marine Science and biological engineeri Qingdao University of Science and Technology 2 0.9046 0.89444 0.09962 0.09876 0.67744 0.67864 0.47577 0.47304 150 150 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   China 2024-07-12 Larval Larval Trunk Surface Structure
33131 33131 SRR29811643 SRX25310831 SRS21984641 SRP519625 PRJNA1132896 Zebrafish larvae exposed by tire wear particles leachate and latex paticles leachate PRJNA1132896 Other This study focuses on investigating the effects of exposure to tire wear particles leachate and latex particles leachate on zebrafish embryos. Specifically we aim to understand how these common environmental pollutants impact the development and health of aquatic organisms with an emphasis on physiological and developmental changes in zebrafish embryos. Our research provides valuable insights into the potential environmental risks associated with tire and latex particle pollutants contributing to the broader field of ecotoxicology and environmental safety.       control 3 CON3   breed:AB|age:120 hours 3|collection date:2024 04 28|geo loc name:China: Qingdao|sex:not collected|tissue:whole body|BioSampleModel:Model organism or animal                 RNA Seq of danio rerio: whole body of larvae LR240511O 1465A LR240511O 1465A mRNA seq of Danio rerio relication 3 of CON     RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina HiSeq 2500   SRP519625     CR3.R1.fq.gz CR3.R2.fq.gz fastq fastq 7149110700.0 23830369.0 CR3.R1.fq.gz 0:150 1:150 A:1820443769;C:1698664767;G:1803666123;T:1826190403;N:145638 150 150     1820443769 1698664767 1803666123 1826190403 145638 SRX25310831 SRS21984641 SRA1918576 Qingdao University of Science and Technology|College of Marine Science and biological engineeri Qingdao University of Science and Technology 2 0.90756 0.89873 0.09562 0.09425 0.67146 0.67359 0.45981 0.46247 150 150 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   China 2024-07-12 Larval Larval Trunk Surface Structure
33132 33132 SRR29811644 SRX25310830 SRS21984640 SRP519625 PRJNA1132896 Zebrafish larvae exposed by tire wear particles leachate and latex paticles leachate PRJNA1132896 Other This study focuses on investigating the effects of exposure to tire wear particles leachate and latex particles leachate on zebrafish embryos. Specifically we aim to understand how these common environmental pollutants impact the development and health of aquatic organisms with an emphasis on physiological and developmental changes in zebrafish embryos. Our research provides valuable insights into the potential environmental risks associated with tire and latex particle pollutants contributing to the broader field of ecotoxicology and environmental safety.       control 2 CON2   breed:AB|age:120 hours 2|collection date:2024 04 28|geo loc name:China: Qingdao|sex:not collected|tissue:whole body|BioSampleModel:Model organism or animal                 RNA Seq of danio rerio: whole body of larvae LR240511O 1464A LR240511O 1464A mRNA seq of Danio rerio relication 2 of CON     RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina HiSeq 2500   SRP519625     CR2.R1.fq.gz CR2.R2.fq.gz fastq fastq 6812733600.0 22709112.0 CR2.R1.fq.gz 0:150 1:150 A:1792158799;C:1525869738;G:1711794485;T:1782779121;N:131457 150 150     1792158799 1525869738 1711794485 1782779121 131457 SRX25310830 SRS21984640 SRA1918576 Qingdao University of Science and Technology|College of Marine Science and biological engineeri Qingdao University of Science and Technology 2 0.89909 0.8788 0.10828 0.10576 0.68335 0.68603 0.46708 0.47979 150 150 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   China 2024-07-12 Larval Larval Trunk Surface Structure
33133 33133 SRR29811645 SRX25310829 SRS21984639 SRP519625 PRJNA1132896 Zebrafish larvae exposed by tire wear particles leachate and latex paticles leachate PRJNA1132896 Other This study focuses on investigating the effects of exposure to tire wear particles leachate and latex particles leachate on zebrafish embryos. Specifically we aim to understand how these common environmental pollutants impact the development and health of aquatic organisms with an emphasis on physiological and developmental changes in zebrafish embryos. Our research provides valuable insights into the potential environmental risks associated with tire and latex particle pollutants contributing to the broader field of ecotoxicology and environmental safety.       control 1 CON1   breed:AB|age:120 hours 1|collection date:2024 04 28|geo loc name:China: Qingdao|sex:not collected|tissue:whole body|BioSampleModel:Model organism or animal                 RNA Seq of danio rerio: whole body of larvae LR240511O 1463A LR240511O 1463A mRNA seq of Danio rerio relication 1 of CON     RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina HiSeq 2500   SRP519625     CR1.R1.fq.gz CR1.R2.fq.gz fastq fastq 6474517800.0 21581726.0 CR1.R1.fq.gz 0:150 1:150 A:1625286788;C:1547447403;G:1668730956;T:1632920740;N:131913 150 150     1625286788 1547447403 1668730956 1632920740 131913 SRX25310829 SRS21984639 SRA1918576 Qingdao University of Science and Technology|College of Marine Science and biological engineeri Qingdao University of Science and Technology 2 0.9112 0.89637 0.08986 0.08847 0.67338 0.67363 0.46785 0.46816 150 150 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   China 2024-07-12 Larval Larval Trunk Surface Structure
36227 36227 SRR33613672 SRX28842027 SRS25072222 SRP586119 PRJNA1263917 Danio rerio Transcriptome or Gene expression PRJNA1263917 Other Analyze the effect of TB KW fermentation on gene expression in high lipid zebrafish.       Danio rerio Danio rerio   strain:missing|isolate:missing|breed:missing|cultivar:missing|ecotype:missing|age:7 day|dev stage:Juvenile fish|collection date:2024 04 16|geo loc name:China: Hang Zhou|sex:missing|tissue:Whole body|BioSampleModel:Model organism or animal                 RNA seq of Danio rerio HFD 3 HFD 3 Total RNA was extracted digested with DNase I and mRNA enriched. post fragmentation cDNA synthesis and library construction the final single stranded DNA nanoball library was generated for sequencing via rolling circle replication.     RNA-Seq TRANSCRIPTOMIC PolyA PAIRED DNBSEQ DNBSEQ-T7   SRP586119     HFD-3-1.fq.gz HFD-3-2.fq.gz fastq fastq 6810755100.0 22702517.0 HFD 3 1.fq.gz 0:150 1:150 A:1804127778;C:1596175018;G:1609090701;T:1801361603;N:0 150 150     1804127778 1596175018 1609090701 1801361603 0 SRX28842027 SRS25072222 SRA2131918 Yibin University|Faculty of Quality Management and Inspection & Qua Yibin University                       B B biological fallback assumption bgi bgi unknown poly_a unknown bulk unknown unknown   China 2025-05-18 Multi-stage Multi-stage Trunk Surface Structure
36228 36228 SRR33613673 SRX28842026 SRS25072222 SRP586119 PRJNA1263917 Danio rerio Transcriptome or Gene expression PRJNA1263917 Other Analyze the effect of TB KW fermentation on gene expression in high lipid zebrafish.       Danio rerio Danio rerio   strain:missing|isolate:missing|breed:missing|cultivar:missing|ecotype:missing|age:7 day|dev stage:Juvenile fish|collection date:2024 04 16|geo loc name:China: Hang Zhou|sex:missing|tissue:Whole body|BioSampleModel:Model organism or animal                 RNA seq of Danio rerio HFD 2 HFD 2 Total RNA was extracted digested with DNase I and mRNA enriched. post fragmentation cDNA synthesis and library construction the final single stranded DNA nanoball library was generated for sequencing via rolling circle replication.     RNA-Seq TRANSCRIPTOMIC PolyA PAIRED DNBSEQ DNBSEQ-T7   SRP586119     HFD-2-1.fq.gz HFD-2-2.fq.gz fastq fastq 6826358400.0 22754528.0 HFD 2 1.fq.gz 0:150 1:150 A:1826465185;C:1582322712;G:1596188986;T:1821381517;N:0 150 150     1826465185 1582322712 1596188986 1821381517 0 SRX28842026 SRS25072222 SRA2131918 Yibin University|Faculty of Quality Management and Inspection & Qua Yibin University                       B B biological fallback assumption bgi bgi unknown poly_a unknown bulk unknown unknown   China 2025-05-18 Multi-stage Multi-stage Trunk Surface Structure
36229 36229 SRR33613674 SRX28842025 SRS25072222 SRP586119 PRJNA1263917 Danio rerio Transcriptome or Gene expression PRJNA1263917 Other Analyze the effect of TB KW fermentation on gene expression in high lipid zebrafish.       Danio rerio Danio rerio   strain:missing|isolate:missing|breed:missing|cultivar:missing|ecotype:missing|age:7 day|dev stage:Juvenile fish|collection date:2024 04 16|geo loc name:China: Hang Zhou|sex:missing|tissue:Whole body|BioSampleModel:Model organism or animal                 RNA seq of Danio rerio HFD 1 HFD 1 Total RNA was extracted digested with DNase I and mRNA enriched. post fragmentation cDNA synthesis and library construction the final single stranded DNA nanoball library was generated for sequencing via rolling circle replication.     RNA-Seq TRANSCRIPTOMIC PolyA PAIRED DNBSEQ DNBSEQ-T7   SRP586119     HFD-1-1.fq.gz HFD-1-2.fq.gz fastq fastq 6598410300.0 21994701.0 HFD 1 1.fq.gz 0:150 1:150 A:1765905584;C:1529581410;G:1542056821;T:1760866485;N:0 150 150     1765905584 1529581410 1542056821 1760866485 0 SRX28842025 SRS25072222 SRA2131918 Yibin University|Faculty of Quality Management and Inspection & Qua Yibin University                       B B biological fallback assumption bgi bgi unknown poly_a unknown bulk unknown unknown   China 2025-05-18 Multi-stage Multi-stage Trunk Surface Structure
36230 36230 SRR33613675 SRX28842024 SRS25072222 SRP586119 PRJNA1263917 Danio rerio Transcriptome or Gene expression PRJNA1263917 Other Analyze the effect of TB KW fermentation on gene expression in high lipid zebrafish.       Danio rerio Danio rerio   strain:missing|isolate:missing|breed:missing|cultivar:missing|ecotype:missing|age:7 day|dev stage:Juvenile fish|collection date:2024 04 16|geo loc name:China: Hang Zhou|sex:missing|tissue:Whole body|BioSampleModel:Model organism or animal                 RNA seq of Danio rerio HFD10 3 HFD10 3 Total RNA was extracted digested with DNase I and mRNA enriched. post fragmentation cDNA synthesis and library construction the final single stranded DNA nanoball library was generated for sequencing via rolling circle replication.     RNA-Seq TRANSCRIPTOMIC PolyA PAIRED DNBSEQ DNBSEQ-T7   SRP586119     HFD10-3-1.fq.gz HFD10-3-2.fq.gz fastq fastq 6609153300.0 22030511.0 HFD10 3 1.fq.gz 0:150 1:150 A:1780787716;C:1521027085;G:1532800826;T:1774537673;N:0 150 150     1780787716 1521027085 1532800826 1774537673 0 SRX28842024 SRS25072222 SRA2131918 Yibin University|Faculty of Quality Management and Inspection & Qua Yibin University                       B B biological fallback assumption bgi bgi unknown poly_a unknown bulk unknown unknown   China 2025-05-18 Multi-stage Multi-stage Trunk Surface Structure
36231 36231 SRR33613676 SRX28842023 SRS25072222 SRP586119 PRJNA1263917 Danio rerio Transcriptome or Gene expression PRJNA1263917 Other Analyze the effect of TB KW fermentation on gene expression in high lipid zebrafish.       Danio rerio Danio rerio   strain:missing|isolate:missing|breed:missing|cultivar:missing|ecotype:missing|age:7 day|dev stage:Juvenile fish|collection date:2024 04 16|geo loc name:China: Hang Zhou|sex:missing|tissue:Whole body|BioSampleModel:Model organism or animal                 RNA seq of Danio rerio HFD10 2 HFD10 2 Total RNA was extracted digested with DNase I and mRNA enriched. post fragmentation cDNA synthesis and library construction the final single stranded DNA nanoball library was generated for sequencing via rolling circle replication.     RNA-Seq TRANSCRIPTOMIC PolyA PAIRED DNBSEQ DNBSEQ-T7   SRP586119     HFD10-2-1.fq.gz HFD10-2-2.fq.gz fastq fastq 6605145900.0 22017153.0 HFD10 2 1.fq.gz 0:150 1:150 A:1765731508;C:1533254667;G:1545960098;T:1760199627;N:0 150 150     1765731508 1533254667 1545960098 1760199627 0 SRX28842023 SRS25072222 SRA2131918 Yibin University|Faculty of Quality Management and Inspection & Qua Yibin University                       B B biological fallback assumption bgi bgi unknown poly_a unknown bulk unknown unknown   China 2025-05-18 Multi-stage Multi-stage Trunk Surface Structure
36232 36232 SRR33613677 SRX28842022 SRS25072222 SRP586119 PRJNA1263917 Danio rerio Transcriptome or Gene expression PRJNA1263917 Other Analyze the effect of TB KW fermentation on gene expression in high lipid zebrafish.       Danio rerio Danio rerio   strain:missing|isolate:missing|breed:missing|cultivar:missing|ecotype:missing|age:7 day|dev stage:Juvenile fish|collection date:2024 04 16|geo loc name:China: Hang Zhou|sex:missing|tissue:Whole body|BioSampleModel:Model organism or animal                 RNA seq of Danio rerio HFD10 1 HFD10 1 Total RNA was extracted digested with DNase I and mRNA enriched. post fragmentation cDNA synthesis and library construction the final single stranded DNA nanoball library was generated for sequencing via rolling circle replication.     RNA-Seq TRANSCRIPTOMIC PolyA PAIRED DNBSEQ DNBSEQ-T7   SRP586119     HFD10-1-1.fq.gz HFD10-1-2.fq.gz fastq fastq 6780056700.0 22600189.0 HFD10 1 1.fq.gz 0:150 1:150 A:1819443100;C:1566081115;G:1580525465;T:1814007020;N:0 150 150     1819443100 1566081115 1580525465 1814007020 0 SRX28842022 SRS25072222 SRA2131918 Yibin University|Faculty of Quality Management and Inspection & Qua Yibin University                       B B biological fallback assumption bgi bgi unknown poly_a unknown bulk unknown unknown   China 2025-05-18 Multi-stage Multi-stage Trunk Surface Structure
36233 36233 SRR33613678 SRX28842021 SRS25072222 SRP586119 PRJNA1263917 Danio rerio Transcriptome or Gene expression PRJNA1263917 Other Analyze the effect of TB KW fermentation on gene expression in high lipid zebrafish.       Danio rerio Danio rerio   strain:missing|isolate:missing|breed:missing|cultivar:missing|ecotype:missing|age:7 day|dev stage:Juvenile fish|collection date:2024 04 16|geo loc name:China: Hang Zhou|sex:missing|tissue:Whole body|BioSampleModel:Model organism or animal                 RNA seq of Danio rerio NFD 3 NFD 3 Total RNA was extracted digested with DNase I and mRNA enriched. post fragmentation cDNA synthesis and library construction the final single stranded DNA nanoball library was generated for sequencing via rolling circle replication.     RNA-Seq TRANSCRIPTOMIC PolyA PAIRED DNBSEQ DNBSEQ-T7   SRP586119     NFD-3-1.fq.gz NFD-3-2.fq.gz fastq fastq 6677775900.0 22259253.0 NFD 3 1.fq.gz 0:150 1:150 A:1794821611;C:1541478212;G:1552820105;T:1788655972;N:0 150 150     1794821611 1541478212 1552820105 1788655972 0 SRX28842021 SRS25072222 SRA2131918 Yibin University|Faculty of Quality Management and Inspection & Qua Yibin University                       B B biological fallback assumption bgi bgi unknown poly_a unknown bulk unknown unknown   China 2025-05-18 Multi-stage Multi-stage Trunk Surface Structure
36234 36234 SRR33613679 SRX28842020 SRS25072222 SRP586119 PRJNA1263917 Danio rerio Transcriptome or Gene expression PRJNA1263917 Other Analyze the effect of TB KW fermentation on gene expression in high lipid zebrafish.       Danio rerio Danio rerio   strain:missing|isolate:missing|breed:missing|cultivar:missing|ecotype:missing|age:7 day|dev stage:Juvenile fish|collection date:2024 04 16|geo loc name:China: Hang Zhou|sex:missing|tissue:Whole body|BioSampleModel:Model organism or animal                 RNA seq of Danio rerio NFD 2 NFD 2 Total RNA was extracted digested with DNase I and mRNA enriched. post fragmentation cDNA synthesis and library construction the final single stranded DNA nanoball library was generated for sequencing via rolling circle replication.     RNA-Seq TRANSCRIPTOMIC PolyA PAIRED DNBSEQ DNBSEQ-T7   SRP586119     NFD-2-1.fq.gz NFD-2-2.fq.gz fastq fastq 6680197200.0 22267324.0 NFD 2 1.fq.gz 0:150 1:150 A:1810744167;C:1526383805;G:1539949310;T:1803119918;N:0 150 150     1810744167 1526383805 1539949310 1803119918 0 SRX28842020 SRS25072222 SRA2131918 Yibin University|Faculty of Quality Management and Inspection & Qua Yibin University                       B B biological fallback assumption bgi bgi unknown poly_a unknown bulk unknown unknown   China 2025-05-18 Multi-stage Multi-stage Trunk Surface Structure
36235 36235 SRR33613680 SRX28842019 SRS25072222 SRP586119 PRJNA1263917 Danio rerio Transcriptome or Gene expression PRJNA1263917 Other Analyze the effect of TB KW fermentation on gene expression in high lipid zebrafish.       Danio rerio Danio rerio   strain:missing|isolate:missing|breed:missing|cultivar:missing|ecotype:missing|age:7 day|dev stage:Juvenile fish|collection date:2024 04 16|geo loc name:China: Hang Zhou|sex:missing|tissue:Whole body|BioSampleModel:Model organism or animal                 RNA seq of Danio rerio NFD 1 NFD 1 Total RNA was extracted digested with DNase I and mRNA enriched. post fragmentation cDNA synthesis and library construction the final single stranded DNA nanoball library was generated for sequencing via rolling circle replication.     RNA-Seq TRANSCRIPTOMIC PolyA PAIRED DNBSEQ DNBSEQ-T7   SRP586119     NFD-1-1.fq.gz NFD-1-2.fq.gz fastq fastq 6587923800.0 21959746.0 NFD 1 1.fq.gz 0:150 1:150 A:1773646514;C:1516320091;G:1530275621;T:1767681574;N:0 150 150     1773646514 1516320091 1530275621 1767681574 0 SRX28842019 SRS25072222 SRA2131918 Yibin University|Faculty of Quality Management and Inspection & Qua Yibin University                       B B biological fallback assumption bgi bgi unknown poly_a unknown bulk unknown unknown   China 2025-05-18 Multi-stage Multi-stage Trunk Surface Structure
53654 53654 SRR10010335 SRX6748603 SRS5298717 SRP219014 PRJNA561212 RNA Seq of nr5a1a and nr5a1b mutant zebrafish PRJNA561212 Transcriptome Analysis Zebrafish were euthanized in Tricaine. We isolated gonad containing trunk of theanimals by removing the anterior of the fish from just posterior of the pectoral finand removing the caudal peduncle posterior to the anus. Trunks were individuallyhomogenized in 200ul Trizol. Total RNA was extracted using the Ribopure RNAPurification Kit ThermoFisher. Total RNA was enriched for mRNA using DynabeadsrOligodt25 ThermoFisher. We constructed indexed strand specific cDNA sequencinglibraries using the NEXTflextm qRNA seq kit BIOO Scientific. Libraryconcentrations were quantified using a Qubitr fluorometer Life Technologies normalized to a concentration of 2.3nM and multiplexed. Prior to sequencing wefurther evaluated the quality of the multiplexed library by quantitative real timePCR using the Kapa Library Quantification Kit Kapa Biosystems. Two lanes ofpaired end 150 base pair bp sequencing were performed on an Illumina HiSeq 4000.         35dpf nr5a1a nr5a1b   strain:AB|age:35 dpf|sex:not applicable|tissue:trunk|biomaterial provider:Postlethwait lab University of Oregon|genotype:nr5a1a / ; nr5a1b / |BioSampleModel:Model organism or animal                 RNA Seq of Danio rerio: 35dpf nr5a1a nr5a1b double mutant 11 35dpf nr5a1a nr5a1b double mutant 11 35dpf nr5a1a nr5a1b double mutant 11 Strand specific RNA seq library prepared with BIOO NEXTflex Rapid Directional qRNA Seq kit     RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina HiSeq 4000   SRP219014     nr5a1ab35dmut-11_combined_R1.fq.gz nr5a1ab35dmut-11_combined_R2.fq.gz fastq fastq 5464020164.0 18092782.0 nr5a1ab35dmut 11 combined R1.fq.gz 0:151 1:151 A:1417197935;C:1238763491;G:1264099371;T:1543026312;N:933055 151 151     1417197935 1238763491 1264099371 1543026312 933055 SRX6748603 SRS5298717 SRA946180 University of Oregon|Institute of Neuroscience University of Oregon 2 0.82573 0.85627 0.0768 0.06148 0.75704 0.76017 0.49566 0.58424 151 151 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   United States 2019-08-21 Juvenile Juvenile Trunk Surface Structure
53655 53655 SRR10010336 SRX6748602 SRS5298716 SRP219014 PRJNA561212 RNA Seq of nr5a1a and nr5a1b mutant zebrafish PRJNA561212 Transcriptome Analysis Zebrafish were euthanized in Tricaine. We isolated gonad containing trunk of theanimals by removing the anterior of the fish from just posterior of the pectoral finand removing the caudal peduncle posterior to the anus. Trunks were individuallyhomogenized in 200ul Trizol. Total RNA was extracted using the Ribopure RNAPurification Kit ThermoFisher. Total RNA was enriched for mRNA using DynabeadsrOligodt25 ThermoFisher. We constructed indexed strand specific cDNA sequencinglibraries using the NEXTflextm qRNA seq kit BIOO Scientific. Libraryconcentrations were quantified using a Qubitr fluorometer Life Technologies normalized to a concentration of 2.3nM and multiplexed. Prior to sequencing wefurther evaluated the quality of the multiplexed library by quantitative real timePCR using the Kapa Library Quantification Kit Kapa Biosystems. Two lanes ofpaired end 150 base pair bp sequencing were performed on an Illumina HiSeq 4000.         35dpf nr5a1b   strain:AB|age:35 dpf|sex:not applicable|tissue:trunk|biomaterial provider:Postlethwait lab University of Oregon|genotype:nr5a1a +/+ ; nr5a1b / |BioSampleModel:Model organism or animal                 RNA Seq of Danio rerio: 35dpf nr5a1b mutant 7 35dpf nr5a1b mutant 7 35dpf nr5a1b mutant 7 Strand specific RNA seq library prepared with BIOO NEXTflex Rapid Directional qRNA Seq kit     RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina HiSeq 4000   SRP219014     nr5a1b35dmut-7_combined_R1.fq.gz nr5a1b35dmut-7_combined_R2.fq.gz fastq fastq 2116406940.0 7007970.0 nr5a1b35dmut 7 combined R1.fq.gz 0:151 1:151 A:534677619;C:494553805;G:503529167;T:583343323;N:303026 151 151     534677619 494553805 503529167 583343323 303026 SRX6748602 SRS5298716 SRA946180 University of Oregon|Institute of Neuroscience University of Oregon 2 0.89953 0.9204 0.08475 0.06359 0.75069 0.75615 0.5203 0.5919 151 151 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   United States 2019-08-21 Juvenile Juvenile Trunk Surface Structure
53656 53656 SRR10010337 SRX6748601 SRS5298717 SRP219014 PRJNA561212 RNA Seq of nr5a1a and nr5a1b mutant zebrafish PRJNA561212 Transcriptome Analysis Zebrafish were euthanized in Tricaine. We isolated gonad containing trunk of theanimals by removing the anterior of the fish from just posterior of the pectoral finand removing the caudal peduncle posterior to the anus. Trunks were individuallyhomogenized in 200ul Trizol. Total RNA was extracted using the Ribopure RNAPurification Kit ThermoFisher. Total RNA was enriched for mRNA using DynabeadsrOligodt25 ThermoFisher. We constructed indexed strand specific cDNA sequencinglibraries using the NEXTflextm qRNA seq kit BIOO Scientific. Libraryconcentrations were quantified using a Qubitr fluorometer Life Technologies normalized to a concentration of 2.3nM and multiplexed. Prior to sequencing wefurther evaluated the quality of the multiplexed library by quantitative real timePCR using the Kapa Library Quantification Kit Kapa Biosystems. Two lanes ofpaired end 150 base pair bp sequencing were performed on an Illumina HiSeq 4000.         35dpf nr5a1a nr5a1b   strain:AB|age:35 dpf|sex:not applicable|tissue:trunk|biomaterial provider:Postlethwait lab University of Oregon|genotype:nr5a1a / ; nr5a1b / |BioSampleModel:Model organism or animal                 RNA Seq of Danio rerio: 35dpf nr5a1a nr5a1b double mutant 10 35dpf nr5a1a nr5a1b double mutant 10 35dpf nr5a1a nr5a1b double mutant 10 Strand specific RNA seq library prepared with BIOO NEXTflex Rapid Directional qRNA Seq kit     RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina HiSeq 4000   SRP219014     nr5a1ab35dmut-10_combined_R1.fq.gz nr5a1ab35dmut-10_combined_R2.fq.gz fastq fastq 3016366034.0 9987967.0 nr5a1ab35dmut 10 combined R1.fq.gz 0:151 1:151 A:752733986;C:720025699;G:731849549;T:811247600;N:509200 151 151     752733986 720025699 731849549 811247600 509200 SRX6748601 SRS5298717 SRA946180 University of Oregon|Institute of Neuroscience University of Oregon 2 0.90615 0.91707 0.11046 0.07981 0.74651 0.74966 0.49676 0.60165 151 151 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   United States 2019-08-21 Juvenile Juvenile Trunk Surface Structure
53657 53657 SRR10010338 SRX6748600 SRS5298717 SRP219014 PRJNA561212 RNA Seq of nr5a1a and nr5a1b mutant zebrafish PRJNA561212 Transcriptome Analysis Zebrafish were euthanized in Tricaine. We isolated gonad containing trunk of theanimals by removing the anterior of the fish from just posterior of the pectoral finand removing the caudal peduncle posterior to the anus. Trunks were individuallyhomogenized in 200ul Trizol. Total RNA was extracted using the Ribopure RNAPurification Kit ThermoFisher. Total RNA was enriched for mRNA using DynabeadsrOligodt25 ThermoFisher. We constructed indexed strand specific cDNA sequencinglibraries using the NEXTflextm qRNA seq kit BIOO Scientific. Libraryconcentrations were quantified using a Qubitr fluorometer Life Technologies normalized to a concentration of 2.3nM and multiplexed. Prior to sequencing wefurther evaluated the quality of the multiplexed library by quantitative real timePCR using the Kapa Library Quantification Kit Kapa Biosystems. Two lanes ofpaired end 150 base pair bp sequencing were performed on an Illumina HiSeq 4000.         35dpf nr5a1a nr5a1b   strain:AB|age:35 dpf|sex:not applicable|tissue:trunk|biomaterial provider:Postlethwait lab University of Oregon|genotype:nr5a1a / ; nr5a1b / |BioSampleModel:Model organism or animal                 RNA Seq of Danio rerio: 35dpf nr5a1a nr5a1b double mutant 9 35dpf nr5a1a nr5a1b double mutant 9 35dpf nr5a1a nr5a1b double mutant 9 Strand specific RNA seq library prepared with BIOO NEXTflex Rapid Directional qRNA Seq kit     RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina HiSeq 4000   SRP219014     nr5a1ab35dmut-9_combined_R1.fq.gz nr5a1ab35dmut-9_combined_R2.fq.gz fastq fastq 3569050194.0 11818047.0 nr5a1ab35dmut 9 combined R1.fq.gz 0:151 1:151 A:899915774;C:849832234;G:859458329;T:959242263;N:601594 151 151     899915774 849832234 859458329 959242263 601594 SRX6748600 SRS5298717 SRA946180 University of Oregon|Institute of Neuroscience University of Oregon 2 0.88978 0.9004 0.09772 0.07218 0.74799 0.74968 0.51174 0.6088 151 151 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   United States 2019-08-21 Juvenile Juvenile Trunk Surface Structure
53658 53658 SRR10010339 SRX6748599 SRS5298717 SRP219014 PRJNA561212 RNA Seq of nr5a1a and nr5a1b mutant zebrafish PRJNA561212 Transcriptome Analysis Zebrafish were euthanized in Tricaine. We isolated gonad containing trunk of theanimals by removing the anterior of the fish from just posterior of the pectoral finand removing the caudal peduncle posterior to the anus. Trunks were individuallyhomogenized in 200ul Trizol. Total RNA was extracted using the Ribopure RNAPurification Kit ThermoFisher. Total RNA was enriched for mRNA using DynabeadsrOligodt25 ThermoFisher. We constructed indexed strand specific cDNA sequencinglibraries using the NEXTflextm qRNA seq kit BIOO Scientific. Libraryconcentrations were quantified using a Qubitr fluorometer Life Technologies normalized to a concentration of 2.3nM and multiplexed. Prior to sequencing wefurther evaluated the quality of the multiplexed library by quantitative real timePCR using the Kapa Library Quantification Kit Kapa Biosystems. Two lanes ofpaired end 150 base pair bp sequencing were performed on an Illumina HiSeq 4000.         35dpf nr5a1a nr5a1b   strain:AB|age:35 dpf|sex:not applicable|tissue:trunk|biomaterial provider:Postlethwait lab University of Oregon|genotype:nr5a1a / ; nr5a1b / |BioSampleModel:Model organism or animal                 RNA Seq of Danio rerio: 35dpf nr5a1a nr5a1b double mutant 2 35dpf nr5a1a nr5a1b double mutant 2 35dpf nr5a1a nr5a1b double mutant 2 Strand specific RNA seq library prepared with BIOO NEXTflex Rapid Directional qRNA Seq kit     RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina HiSeq 4000   SRP219014     nr5a1ab35dmut-2_combined_R1.fq.gz nr5a1ab35dmut-2_combined_R2.fq.gz fastq fastq 2828323922.0 9365311.0 nr5a1ab35dmut 2 combined R1.fq.gz 0:151 1:151 A:691090279;C:673032167;G:684529355;T:779155559;N:516562 151 151     691090279 673032167 684529355 779155559 516562 SRX6748599 SRS5298717 SRA946180 University of Oregon|Institute of Neuroscience University of Oregon 2 0.8834 0.9068 0.11546 0.07455 0.75503 0.75615 0.51068 0.61929 151 151 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   United States 2019-08-21 Juvenile Juvenile Trunk Surface Structure
53659 53659 SRR10010340 SRX6748598 SRS5298717 SRP219014 PRJNA561212 RNA Seq of nr5a1a and nr5a1b mutant zebrafish PRJNA561212 Transcriptome Analysis Zebrafish were euthanized in Tricaine. We isolated gonad containing trunk of theanimals by removing the anterior of the fish from just posterior of the pectoral finand removing the caudal peduncle posterior to the anus. Trunks were individuallyhomogenized in 200ul Trizol. Total RNA was extracted using the Ribopure RNAPurification Kit ThermoFisher. Total RNA was enriched for mRNA using DynabeadsrOligodt25 ThermoFisher. We constructed indexed strand specific cDNA sequencinglibraries using the NEXTflextm qRNA seq kit BIOO Scientific. Libraryconcentrations were quantified using a Qubitr fluorometer Life Technologies normalized to a concentration of 2.3nM and multiplexed. Prior to sequencing wefurther evaluated the quality of the multiplexed library by quantitative real timePCR using the Kapa Library Quantification Kit Kapa Biosystems. Two lanes ofpaired end 150 base pair bp sequencing were performed on an Illumina HiSeq 4000.         35dpf nr5a1a nr5a1b   strain:AB|age:35 dpf|sex:not applicable|tissue:trunk|biomaterial provider:Postlethwait lab University of Oregon|genotype:nr5a1a / ; nr5a1b / |BioSampleModel:Model organism or animal                 RNA Seq of Danio rerio: 35dpf nr5a1a nr5a1b double mutant 1 35dpf nr5a1a nr5a1b double mutant 1 35dpf nr5a1a nr5a1b double mutant 1 Strand specific RNA seq library prepared with BIOO NEXTflex Rapid Directional qRNA Seq kit     RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina HiSeq 4000   SRP219014     nr5a1ab35dmut-1_combined_R1.fq.gz nr5a1ab35dmut-1_combined_R2.fq.gz fastq fastq 3461567790.0 11462145.0 nr5a1ab35dmut 1 combined R1.fq.gz 0:151 1:151 A:863912807;C:826288038;G:844009627;T:926819831;N:537487 151 151     863912807 826288038 844009627 926819831 537487 SRX6748598 SRS5298717 SRA946180 University of Oregon|Institute of Neuroscience University of Oregon 2 0.90732 0.91778 0.10103 0.06771 0.74026 0.74619 0.50631 0.59632 151 151 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   United States 2019-08-21 Juvenile Juvenile Trunk Surface Structure
53660 53660 SRR10010341 SRX6748597 SRS5298716 SRP219014 PRJNA561212 RNA Seq of nr5a1a and nr5a1b mutant zebrafish PRJNA561212 Transcriptome Analysis Zebrafish were euthanized in Tricaine. We isolated gonad containing trunk of theanimals by removing the anterior of the fish from just posterior of the pectoral finand removing the caudal peduncle posterior to the anus. Trunks were individuallyhomogenized in 200ul Trizol. Total RNA was extracted using the Ribopure RNAPurification Kit ThermoFisher. Total RNA was enriched for mRNA using DynabeadsrOligodt25 ThermoFisher. We constructed indexed strand specific cDNA sequencinglibraries using the NEXTflextm qRNA seq kit BIOO Scientific. Libraryconcentrations were quantified using a Qubitr fluorometer Life Technologies normalized to a concentration of 2.3nM and multiplexed. Prior to sequencing wefurther evaluated the quality of the multiplexed library by quantitative real timePCR using the Kapa Library Quantification Kit Kapa Biosystems. Two lanes ofpaired end 150 base pair bp sequencing were performed on an Illumina HiSeq 4000.         35dpf nr5a1b   strain:AB|age:35 dpf|sex:not applicable|tissue:trunk|biomaterial provider:Postlethwait lab University of Oregon|genotype:nr5a1a +/+ ; nr5a1b / |BioSampleModel:Model organism or animal                 RNA Seq of Danio rerio: 35dpf nr5a1b mutant 4 35dpf nr5a1b mutant 4 35dpf nr5a1b mutant 4 Strand specific RNA seq library prepared with BIOO NEXTflex Rapid Directional qRNA Seq kit     RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina HiSeq 4000   SRP219014     nr5a1b35dmut-4_combined_R1.fq.gz nr5a1b35dmut-4_combined_R2.fq.gz fastq fastq 2823289582.0 9348641.0 nr5a1b35dmut 4 combined R1.fq.gz 0:151 1:151 A:692918043;C:667773087;G:683991041;T:778117057;N:490354 151 151     692918043 667773087 683991041 778117057 490354 SRX6748597 SRS5298716 SRA946180 University of Oregon|Institute of Neuroscience University of Oregon 2 0.89649 0.92027 0.09415 0.06385 0.75463 0.75883 0.48098 0.58128 151 151 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   United States 2019-08-21 Juvenile Juvenile Trunk Surface Structure
53661 53661 SRR10010342 SRX6748596 SRS5298717 SRP219014 PRJNA561212 RNA Seq of nr5a1a and nr5a1b mutant zebrafish PRJNA561212 Transcriptome Analysis Zebrafish were euthanized in Tricaine. We isolated gonad containing trunk of theanimals by removing the anterior of the fish from just posterior of the pectoral finand removing the caudal peduncle posterior to the anus. Trunks were individuallyhomogenized in 200ul Trizol. Total RNA was extracted using the Ribopure RNAPurification Kit ThermoFisher. Total RNA was enriched for mRNA using DynabeadsrOligodt25 ThermoFisher. We constructed indexed strand specific cDNA sequencinglibraries using the NEXTflextm qRNA seq kit BIOO Scientific. Libraryconcentrations were quantified using a Qubitr fluorometer Life Technologies normalized to a concentration of 2.3nM and multiplexed. Prior to sequencing wefurther evaluated the quality of the multiplexed library by quantitative real timePCR using the Kapa Library Quantification Kit Kapa Biosystems. Two lanes ofpaired end 150 base pair bp sequencing were performed on an Illumina HiSeq 4000.         35dpf nr5a1a nr5a1b   strain:AB|age:35 dpf|sex:not applicable|tissue:trunk|biomaterial provider:Postlethwait lab University of Oregon|genotype:nr5a1a / ; nr5a1b / |BioSampleModel:Model organism or animal                 RNA Seq of Danio rerio: 35dpf nr5a1a nr5a1b double mutant 14 35dpf nr5a1a nr5a1b double mutant 14 35dpf nr5a1a nr5a1b double mutant 14 Strand specific RNA seq library prepared with BIOO NEXTflex Rapid Directional qRNA Seq kit     RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina HiSeq 4000   SRP219014     nr5a1ab35dmut-14_combined_R1.fq.gz nr5a1ab35dmut-14_combined_R2.fq.gz fastq fastq 2662720410.0 8816955.0 nr5a1ab35dmut 14 combined R1.fq.gz 0:151 1:151 A:665577500;C:635869780;G:647241076;T:713592928;N:439126 151 151     665577500 635869780 647241076 713592928 439126 SRX6748596 SRS5298717 SRA946180 University of Oregon|Institute of Neuroscience University of Oregon 2 0.90062 0.91305 0.1132 0.08326 0.75339 0.7555 0.49702 0.59781 151 151 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   United States 2019-08-21 Juvenile Juvenile Trunk Surface Structure
53662 53662 SRR10010343 SRX6748595 SRS5298720 SRP219014 PRJNA561212 RNA Seq of nr5a1a and nr5a1b mutant zebrafish PRJNA561212 Transcriptome Analysis Zebrafish were euthanized in Tricaine. We isolated gonad containing trunk of theanimals by removing the anterior of the fish from just posterior of the pectoral finand removing the caudal peduncle posterior to the anus. Trunks were individuallyhomogenized in 200ul Trizol. Total RNA was extracted using the Ribopure RNAPurification Kit ThermoFisher. Total RNA was enriched for mRNA using DynabeadsrOligodt25 ThermoFisher. We constructed indexed strand specific cDNA sequencinglibraries using the NEXTflextm qRNA seq kit BIOO Scientific. Libraryconcentrations were quantified using a Qubitr fluorometer Life Technologies normalized to a concentration of 2.3nM and multiplexed. Prior to sequencing wefurther evaluated the quality of the multiplexed library by quantitative real timePCR using the Kapa Library Quantification Kit Kapa Biosystems. Two lanes ofpaired end 150 base pair bp sequencing were performed on an Illumina HiSeq 4000.         21dpf nr5a1b   strain:AB|age:21 dpf|sex:not applicable|tissue:trunk|biomaterial provider:Postlethwait lab University of Oregon|genotype:nr5a1a +/+ ; nr5a1b / |BioSampleModel:Model organism or animal                 RNA Seq of Danio rerio: 21dpf nr5a1b mutant 7 21dpf nr5a1b mutant 7 21dpf nr5a1b mutant 7 Strand specific RNA seq library prepared with BIOO NEXTflex Rapid Directional qRNA Seq kit     RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina HiSeq 4000   SRP219014     nr5a1b21dmut-7_combined_R1.fq.gz nr5a1b21dmut-7_combined_R2.fq.gz fastq fastq 3413772968.0 11303884.0 nr5a1b21dmut 7 combined R1.fq.gz 0:151 1:151 A:866277297;C:793136535;G:808490993;T:945306139;N:562004 151 151     866277297 793136535 808490993 945306139 562004 SRX6748595 SRS5298720 SRA946180 University of Oregon|Institute of Neuroscience University of Oregon 2 0.90038 0.9162 0.09303 0.06982 0.74637 0.74738 0.49835 0.58029 151 151 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   United States 2019-08-21 Larval Larval Trunk Surface Structure
53663 53663 SRR10010344 SRX6748594 SRS5298720 SRP219014 PRJNA561212 RNA Seq of nr5a1a and nr5a1b mutant zebrafish PRJNA561212 Transcriptome Analysis Zebrafish were euthanized in Tricaine. We isolated gonad containing trunk of theanimals by removing the anterior of the fish from just posterior of the pectoral finand removing the caudal peduncle posterior to the anus. Trunks were individuallyhomogenized in 200ul Trizol. Total RNA was extracted using the Ribopure RNAPurification Kit ThermoFisher. Total RNA was enriched for mRNA using DynabeadsrOligodt25 ThermoFisher. We constructed indexed strand specific cDNA sequencinglibraries using the NEXTflextm qRNA seq kit BIOO Scientific. Libraryconcentrations were quantified using a Qubitr fluorometer Life Technologies normalized to a concentration of 2.3nM and multiplexed. Prior to sequencing wefurther evaluated the quality of the multiplexed library by quantitative real timePCR using the Kapa Library Quantification Kit Kapa Biosystems. Two lanes ofpaired end 150 base pair bp sequencing were performed on an Illumina HiSeq 4000.         21dpf nr5a1b   strain:AB|age:21 dpf|sex:not applicable|tissue:trunk|biomaterial provider:Postlethwait lab University of Oregon|genotype:nr5a1a +/+ ; nr5a1b / |BioSampleModel:Model organism or animal                 RNA Seq of Danio rerio: 21dpf nr5a1b mutant 8 21dpf nr5a1b mutant 8 21dpf nr5a1b mutant 8 Strand specific RNA seq library prepared with BIOO NEXTflex Rapid Directional qRNA Seq kit     RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina HiSeq 4000   SRP219014     nr5a1b21dmut-8_combined_R1.fq.gz nr5a1b21dmut-8_combined_R2.fq.gz fastq fastq 4840911718.0 16029509.0 nr5a1b21dmut 8 combined R1.fq.gz 0:151 1:151 A:1202544092;C:1166760381;G:1186843611;T:1283964664;N:798970 151 151     1202544092 1166760381 1186843611 1283964664 798970 SRX6748594 SRS5298720 SRA946180 University of Oregon|Institute of Neuroscience University of Oregon 2 0.9056 0.91348 0.12201 0.08631 0.72845 0.73336 0.45147 0.53636 151 151 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   United States 2019-08-21 Larval Larval Trunk Surface Structure
53664 53664 SRR10010345 SRX6748593 SRS5298716 SRP219014 PRJNA561212 RNA Seq of nr5a1a and nr5a1b mutant zebrafish PRJNA561212 Transcriptome Analysis Zebrafish were euthanized in Tricaine. We isolated gonad containing trunk of theanimals by removing the anterior of the fish from just posterior of the pectoral finand removing the caudal peduncle posterior to the anus. Trunks were individuallyhomogenized in 200ul Trizol. Total RNA was extracted using the Ribopure RNAPurification Kit ThermoFisher. Total RNA was enriched for mRNA using DynabeadsrOligodt25 ThermoFisher. We constructed indexed strand specific cDNA sequencinglibraries using the NEXTflextm qRNA seq kit BIOO Scientific. Libraryconcentrations were quantified using a Qubitr fluorometer Life Technologies normalized to a concentration of 2.3nM and multiplexed. Prior to sequencing wefurther evaluated the quality of the multiplexed library by quantitative real timePCR using the Kapa Library Quantification Kit Kapa Biosystems. Two lanes ofpaired end 150 base pair bp sequencing were performed on an Illumina HiSeq 4000.         35dpf nr5a1b   strain:AB|age:35 dpf|sex:not applicable|tissue:trunk|biomaterial provider:Postlethwait lab University of Oregon|genotype:nr5a1a +/+ ; nr5a1b / |BioSampleModel:Model organism or animal                 RNA Seq of Danio rerio: 35dpf nr5a1b mutant 1 35dpf nr5a1b mutant 1 35dpf nr5a1b mutant 1 Strand specific RNA seq library prepared with BIOO NEXTflex Rapid Directional qRNA Seq kit     RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina HiSeq 4000   SRP219014     nr5a1b35dmut-1_combined_R1.fq.gz nr5a1b35dmut-1_combined_R2.fq.gz fastq fastq 3080371310.0 10199905.0 nr5a1b35dmut 1 combined R1.fq.gz 0:151 1:151 A:749979603;C:751889973;G:770422909;T:807597821;N:481004 151 151     749979603 751889973 770422909 807597821 481004 SRX6748593 SRS5298716 SRA946180 University of Oregon|Institute of Neuroscience University of Oregon 2 0.91959 0.93095 0.11586 0.07628 0.76187 0.76562 0.49454 0.57785 151 151 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   United States 2019-08-21 Juvenile Juvenile Trunk Surface Structure
53665 53665 SRR10010346 SRX6748592 SRS5298716 SRP219014 PRJNA561212 RNA Seq of nr5a1a and nr5a1b mutant zebrafish PRJNA561212 Transcriptome Analysis Zebrafish were euthanized in Tricaine. We isolated gonad containing trunk of theanimals by removing the anterior of the fish from just posterior of the pectoral finand removing the caudal peduncle posterior to the anus. Trunks were individuallyhomogenized in 200ul Trizol. Total RNA was extracted using the Ribopure RNAPurification Kit ThermoFisher. Total RNA was enriched for mRNA using DynabeadsrOligodt25 ThermoFisher. We constructed indexed strand specific cDNA sequencinglibraries using the NEXTflextm qRNA seq kit BIOO Scientific. Libraryconcentrations were quantified using a Qubitr fluorometer Life Technologies normalized to a concentration of 2.3nM and multiplexed. Prior to sequencing wefurther evaluated the quality of the multiplexed library by quantitative real timePCR using the Kapa Library Quantification Kit Kapa Biosystems. Two lanes ofpaired end 150 base pair bp sequencing were performed on an Illumina HiSeq 4000.         35dpf nr5a1b   strain:AB|age:35 dpf|sex:not applicable|tissue:trunk|biomaterial provider:Postlethwait lab University of Oregon|genotype:nr5a1a +/+ ; nr5a1b / |BioSampleModel:Model organism or animal                 RNA Seq of Danio rerio: 35dpf nr5a1b mutant 2 35dpf nr5a1b mutant 2 35dpf nr5a1b mutant 2 Strand specific RNA seq library prepared with BIOO NEXTflex Rapid Directional qRNA Seq kit     RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina HiSeq 4000   SRP219014     nr5a1b35dmut-2_combined_R1.fq.gz nr5a1b35dmut-2_combined_R2.fq.gz fastq fastq 3633938820.0 12032910.0 nr5a1b35dmut 2 combined R1.fq.gz 0:151 1:151 A:905316417;C:865468187;G:876812140;T:985720508;N:621568 151 151     905316417 865468187 876812140 985720508 621568 SRX6748592 SRS5298716 SRA946180 University of Oregon|Institute of Neuroscience University of Oregon 2 0.8972 0.91027 0.11437 0.07781 0.74099 0.74446 0.4981 0.60461 151 151 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   United States 2019-08-21 Juvenile Juvenile Trunk Surface Structure
53666 53666 SRR10010347 SRX6748591 SRS5298720 SRP219014 PRJNA561212 RNA Seq of nr5a1a and nr5a1b mutant zebrafish PRJNA561212 Transcriptome Analysis Zebrafish were euthanized in Tricaine. We isolated gonad containing trunk of theanimals by removing the anterior of the fish from just posterior of the pectoral finand removing the caudal peduncle posterior to the anus. Trunks were individuallyhomogenized in 200ul Trizol. Total RNA was extracted using the Ribopure RNAPurification Kit ThermoFisher. Total RNA was enriched for mRNA using DynabeadsrOligodt25 ThermoFisher. We constructed indexed strand specific cDNA sequencinglibraries using the NEXTflextm qRNA seq kit BIOO Scientific. Libraryconcentrations were quantified using a Qubitr fluorometer Life Technologies normalized to a concentration of 2.3nM and multiplexed. Prior to sequencing wefurther evaluated the quality of the multiplexed library by quantitative real timePCR using the Kapa Library Quantification Kit Kapa Biosystems. Two lanes ofpaired end 150 base pair bp sequencing were performed on an Illumina HiSeq 4000.         21dpf nr5a1b   strain:AB|age:21 dpf|sex:not applicable|tissue:trunk|biomaterial provider:Postlethwait lab University of Oregon|genotype:nr5a1a +/+ ; nr5a1b / |BioSampleModel:Model organism or animal                 RNA Seq of Danio rerio: 21dpf nr5a1b mutant 3 21dpf nr5a1b mutant 3 21dpf nr5a1b mutant 3 Strand specific RNA seq library prepared with BIOO NEXTflex Rapid Directional qRNA Seq kit     RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina HiSeq 4000   SRP219014     nr5a1b21dmut-3_combined_R1.fq.gz nr5a1b21dmut-3_combined_R2.fq.gz fastq fastq 2010998370.0 6658935.0 nr5a1b21dmut 3 combined R1.fq.gz 0:151 1:151 A:510741274;C:461058309;G:465853553;T:573016929;N:328305 151 151     510741274 461058309 465853553 573016929 328305 SRX6748591 SRS5298720 SRA946180 University of Oregon|Institute of Neuroscience University of Oregon 2 0.87512 0.8951 0.10891 0.08286 0.74759 0.74882 0.51531 0.57614 151 151 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   United States 2019-08-21 Larval Larval Trunk Surface Structure
53667 53667 SRR10010348 SRX6748590 SRS5298720 SRP219014 PRJNA561212 RNA Seq of nr5a1a and nr5a1b mutant zebrafish PRJNA561212 Transcriptome Analysis Zebrafish were euthanized in Tricaine. We isolated gonad containing trunk of theanimals by removing the anterior of the fish from just posterior of the pectoral finand removing the caudal peduncle posterior to the anus. Trunks were individuallyhomogenized in 200ul Trizol. Total RNA was extracted using the Ribopure RNAPurification Kit ThermoFisher. Total RNA was enriched for mRNA using DynabeadsrOligodt25 ThermoFisher. We constructed indexed strand specific cDNA sequencinglibraries using the NEXTflextm qRNA seq kit BIOO Scientific. Libraryconcentrations were quantified using a Qubitr fluorometer Life Technologies normalized to a concentration of 2.3nM and multiplexed. Prior to sequencing wefurther evaluated the quality of the multiplexed library by quantitative real timePCR using the Kapa Library Quantification Kit Kapa Biosystems. Two lanes ofpaired end 150 base pair bp sequencing were performed on an Illumina HiSeq 4000.         21dpf nr5a1b   strain:AB|age:21 dpf|sex:not applicable|tissue:trunk|biomaterial provider:Postlethwait lab University of Oregon|genotype:nr5a1a +/+ ; nr5a1b / |BioSampleModel:Model organism or animal                 RNA Seq of Danio rerio: 21dpf nr5a1b mutant 4 21dpf nr5a1b mutant 4 21dpf nr5a1b mutant 4 Strand specific RNA seq library prepared with BIOO NEXTflex Rapid Directional qRNA Seq kit     RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina HiSeq 4000   SRP219014     nr5a1b21dmut-4_combined_R1.fq.gz nr5a1b21dmut-4_combined_R2.fq.gz fastq fastq 2838303512.0 9398356.0 nr5a1b21dmut 4 combined R1.fq.gz 0:151 1:151 A:696399092;C:677565241;G:694174849;T:769657171;N:507159 151 151     696399092 677565241 694174849 769657171 507159 SRX6748590 SRS5298720 SRA946180 University of Oregon|Institute of Neuroscience University of Oregon 2 0.88814 0.90692 0.10219 0.06896 0.74207 0.74186 0.47224 0.56972 151 151 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   United States 2019-08-21 Larval Larval Trunk Surface Structure
53668 53668 SRR10010349 SRX6748589 SRS5298723 SRP219014 PRJNA561212 RNA Seq of nr5a1a and nr5a1b mutant zebrafish PRJNA561212 Transcriptome Analysis Zebrafish were euthanized in Tricaine. We isolated gonad containing trunk of theanimals by removing the anterior of the fish from just posterior of the pectoral finand removing the caudal peduncle posterior to the anus. Trunks were individuallyhomogenized in 200ul Trizol. Total RNA was extracted using the Ribopure RNAPurification Kit ThermoFisher. Total RNA was enriched for mRNA using DynabeadsrOligodt25 ThermoFisher. We constructed indexed strand specific cDNA sequencinglibraries using the NEXTflextm qRNA seq kit BIOO Scientific. Libraryconcentrations were quantified using a Qubitr fluorometer Life Technologies normalized to a concentration of 2.3nM and multiplexed. Prior to sequencing wefurther evaluated the quality of the multiplexed library by quantitative real timePCR using the Kapa Library Quantification Kit Kapa Biosystems. Two lanes ofpaired end 150 base pair bp sequencing were performed on an Illumina HiSeq 4000.         21dpf nr5a1a nr5a1b   strain:AB|age:21 dpf|sex:not applicable|tissue:trunk|biomaterial provider:Postlethwait lab University of Oregon|genotype:nr5a1a / ; nr5a1b / |BioSampleModel:Model organism or animal                 RNA Seq of Danio rerio: 21dpf nr5a1a nr5a1b double mutant 7 21dpf nr5a1a nr5a1b double mutant 7 21dpf nr5a1a nr5a1b double mutant 7 Strand specific RNA seq library prepared with BIOO NEXTflex Rapid Directional qRNA Seq kit     RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina HiSeq 4000   SRP219014     nr5a1ab21dmut-7_combined_R1.fq.gz nr5a1ab21dmut-7_combined_R2.fq.gz fastq fastq 4301143494.0 14242197.0 nr5a1ab21dmut 7 combined R1.fq.gz 0:151 1:151 A:1037292424;C:1070782216;G:1085961255;T:1106426728;N:680871 151 151     1037292424 1070782216 1085961255 1106426728 680871 SRX6748589 SRS5298723 SRA946180 University of Oregon|Institute of Neuroscience University of Oregon 2 0.92203 0.92848 0.14359 0.09024 0.76449 0.76852 0.45779 0.57313 151 151 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   United States 2019-08-21 Larval Larval Trunk Surface Structure
53669 53669 SRR10010350 SRX6748588 SRS5298723 SRP219014 PRJNA561212 RNA Seq of nr5a1a and nr5a1b mutant zebrafish PRJNA561212 Transcriptome Analysis Zebrafish were euthanized in Tricaine. We isolated gonad containing trunk of theanimals by removing the anterior of the fish from just posterior of the pectoral finand removing the caudal peduncle posterior to the anus. Trunks were individuallyhomogenized in 200ul Trizol. Total RNA was extracted using the Ribopure RNAPurification Kit ThermoFisher. Total RNA was enriched for mRNA using DynabeadsrOligodt25 ThermoFisher. We constructed indexed strand specific cDNA sequencinglibraries using the NEXTflextm qRNA seq kit BIOO Scientific. Libraryconcentrations were quantified using a Qubitr fluorometer Life Technologies normalized to a concentration of 2.3nM and multiplexed. Prior to sequencing wefurther evaluated the quality of the multiplexed library by quantitative real timePCR using the Kapa Library Quantification Kit Kapa Biosystems. Two lanes ofpaired end 150 base pair bp sequencing were performed on an Illumina HiSeq 4000.         21dpf nr5a1a nr5a1b   strain:AB|age:21 dpf|sex:not applicable|tissue:trunk|biomaterial provider:Postlethwait lab University of Oregon|genotype:nr5a1a / ; nr5a1b / |BioSampleModel:Model organism or animal                 RNA Seq of Danio rerio: 21dpf nr5a1a nr5a1b double mutant 8 21dpf nr5a1a nr5a1b double mutant 8 21dpf nr5a1a nr5a1b double mutant 8 Strand specific RNA seq library prepared with BIOO NEXTflex Rapid Directional qRNA Seq kit     RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina HiSeq 4000   SRP219014     nr5a1ab21dmut-8_combined_R1.fq.gz nr5a1ab21dmut-8_combined_R2.fq.gz fastq fastq 3463183188.0 11467494.0 nr5a1ab21dmut 8 combined R1.fq.gz 0:151 1:151 A:873871340;C:824464048;G:837882345;T:926409947;N:555508 151 151     873871340 824464048 837882345 926409947 555508 SRX6748588 SRS5298723 SRA946180 University of Oregon|Institute of Neuroscience University of Oregon 2 0.90477 0.9091 0.09733 0.07335 0.73768 0.74083 0.47337 0.54642 151 151 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   United States 2019-08-21 Larval Larval Trunk Surface Structure
53670 53670 SRR10010351 SRX6748587 SRS5298723 SRP219014 PRJNA561212 RNA Seq of nr5a1a and nr5a1b mutant zebrafish PRJNA561212 Transcriptome Analysis Zebrafish were euthanized in Tricaine. We isolated gonad containing trunk of theanimals by removing the anterior of the fish from just posterior of the pectoral finand removing the caudal peduncle posterior to the anus. Trunks were individuallyhomogenized in 200ul Trizol. Total RNA was extracted using the Ribopure RNAPurification Kit ThermoFisher. Total RNA was enriched for mRNA using DynabeadsrOligodt25 ThermoFisher. We constructed indexed strand specific cDNA sequencinglibraries using the NEXTflextm qRNA seq kit BIOO Scientific. Libraryconcentrations were quantified using a Qubitr fluorometer Life Technologies normalized to a concentration of 2.3nM and multiplexed. Prior to sequencing wefurther evaluated the quality of the multiplexed library by quantitative real timePCR using the Kapa Library Quantification Kit Kapa Biosystems. Two lanes ofpaired end 150 base pair bp sequencing were performed on an Illumina HiSeq 4000.         21dpf nr5a1a nr5a1b   strain:AB|age:21 dpf|sex:not applicable|tissue:trunk|biomaterial provider:Postlethwait lab University of Oregon|genotype:nr5a1a / ; nr5a1b / |BioSampleModel:Model organism or animal                 RNA Seq of Danio rerio: 21dpf nr5a1a nr5a1b double mutant 5 21dpf nr5a1a nr5a1b double mutant 5 21dpf nr5a1a nr5a1b double mutant 5 Strand specific RNA seq library prepared with BIOO NEXTflex Rapid Directional qRNA Seq kit     RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina HiSeq 4000   SRP219014     nr5a1ab21dmut-5_combined_R1.fq.gz nr5a1ab21dmut-5_combined_R2.fq.gz fastq fastq 2105581146.0 6972123.0 nr5a1ab21dmut 5 combined R1.fq.gz 0:151 1:151 A:539297149;C:475252041;G:487324441;T:603338432;N:369083 151 151     539297149 475252041 487324441 603338432 369083 SRX6748587 SRS5298723 SRA946180 University of Oregon|Institute of Neuroscience University of Oregon 2 0.83588 0.86367 0.07285 0.05907 0.74795 0.74789 0.50471 0.57306 151 151 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   United States 2019-08-21 Larval Larval Trunk Surface Structure
53671 53671 SRR10010352 SRX6748586 SRS5298723 SRP219014 PRJNA561212 RNA Seq of nr5a1a and nr5a1b mutant zebrafish PRJNA561212 Transcriptome Analysis Zebrafish were euthanized in Tricaine. We isolated gonad containing trunk of theanimals by removing the anterior of the fish from just posterior of the pectoral finand removing the caudal peduncle posterior to the anus. Trunks were individuallyhomogenized in 200ul Trizol. Total RNA was extracted using the Ribopure RNAPurification Kit ThermoFisher. Total RNA was enriched for mRNA using DynabeadsrOligodt25 ThermoFisher. We constructed indexed strand specific cDNA sequencinglibraries using the NEXTflextm qRNA seq kit BIOO Scientific. Libraryconcentrations were quantified using a Qubitr fluorometer Life Technologies normalized to a concentration of 2.3nM and multiplexed. Prior to sequencing wefurther evaluated the quality of the multiplexed library by quantitative real timePCR using the Kapa Library Quantification Kit Kapa Biosystems. Two lanes ofpaired end 150 base pair bp sequencing were performed on an Illumina HiSeq 4000.         21dpf nr5a1a nr5a1b   strain:AB|age:21 dpf|sex:not applicable|tissue:trunk|biomaterial provider:Postlethwait lab University of Oregon|genotype:nr5a1a / ; nr5a1b / |BioSampleModel:Model organism or animal                 RNA Seq of Danio rerio: 21dpf nr5a1a nr5a1b double mutant 6 21dpf nr5a1a nr5a1b double mutant 6 21dpf nr5a1a nr5a1b double mutant 6 Strand specific RNA seq library prepared with BIOO NEXTflex Rapid Directional qRNA Seq kit     RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina HiSeq 4000   SRP219014     nr5a1ab21dmut-6_combined_R1.fq.gz nr5a1ab21dmut-6_combined_R2.fq.gz fastq fastq 2160567192.0 7154196.0 nr5a1ab21dmut 6 combined R1.fq.gz 0:151 1:151 A:549656125;C:499912370;G:508304192;T:602348154;N:346351 151 151     549656125 499912370 508304192 602348154 346351 SRX6748586 SRS5298723 SRA946180 University of Oregon|Institute of Neuroscience University of Oregon 2 0.88824 0.90531 0.11055 0.0875 0.70554 0.70859 0.48379 0.54732 151 151 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   United States 2019-08-21 Larval Larval Trunk Surface Structure
53672 53672 SRR10010353 SRX6748585 SRS5298723 SRP219014 PRJNA561212 RNA Seq of nr5a1a and nr5a1b mutant zebrafish PRJNA561212 Transcriptome Analysis Zebrafish were euthanized in Tricaine. We isolated gonad containing trunk of theanimals by removing the anterior of the fish from just posterior of the pectoral finand removing the caudal peduncle posterior to the anus. Trunks were individuallyhomogenized in 200ul Trizol. Total RNA was extracted using the Ribopure RNAPurification Kit ThermoFisher. Total RNA was enriched for mRNA using DynabeadsrOligodt25 ThermoFisher. We constructed indexed strand specific cDNA sequencinglibraries using the NEXTflextm qRNA seq kit BIOO Scientific. Libraryconcentrations were quantified using a Qubitr fluorometer Life Technologies normalized to a concentration of 2.3nM and multiplexed. Prior to sequencing wefurther evaluated the quality of the multiplexed library by quantitative real timePCR using the Kapa Library Quantification Kit Kapa Biosystems. Two lanes ofpaired end 150 base pair bp sequencing were performed on an Illumina HiSeq 4000.         21dpf nr5a1a nr5a1b   strain:AB|age:21 dpf|sex:not applicable|tissue:trunk|biomaterial provider:Postlethwait lab University of Oregon|genotype:nr5a1a / ; nr5a1b / |BioSampleModel:Model organism or animal                 RNA Seq of Danio rerio: 21dpf nr5a1a nr5a1b double mutant 3 21dpf nr5a1a nr5a1b double mutant 3 21dpf nr5a1a nr5a1b double mutant 3 Strand specific RNA seq library prepared with BIOO NEXTflex Rapid Directional qRNA Seq kit     RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina HiSeq 4000   SRP219014     nr5a1ab21dmut-3_combined_R1.fq.gz nr5a1ab21dmut-3_combined_R2.fq.gz fastq fastq 1850334974.0 6126937.0 nr5a1ab21dmut 3 combined R1.fq.gz 0:151 1:151 A:470148849;C:425157376;G:428614793;T:526090487;N:323469 151 151     470148849 425157376 428614793 526090487 323469 SRX6748585 SRS5298723 SRA946180 University of Oregon|Institute of Neuroscience University of Oregon 2 0.86747 0.88781 0.13442 0.09954 0.7514 0.75091 0.4841 0.58575 151 151 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   United States 2019-08-21 Larval Larval Trunk Surface Structure
53673 53673 SRR10010354 SRX6748584 SRS5298723 SRP219014 PRJNA561212 RNA Seq of nr5a1a and nr5a1b mutant zebrafish PRJNA561212 Transcriptome Analysis Zebrafish were euthanized in Tricaine. We isolated gonad containing trunk of theanimals by removing the anterior of the fish from just posterior of the pectoral finand removing the caudal peduncle posterior to the anus. Trunks were individuallyhomogenized in 200ul Trizol. Total RNA was extracted using the Ribopure RNAPurification Kit ThermoFisher. Total RNA was enriched for mRNA using DynabeadsrOligodt25 ThermoFisher. We constructed indexed strand specific cDNA sequencinglibraries using the NEXTflextm qRNA seq kit BIOO Scientific. Libraryconcentrations were quantified using a Qubitr fluorometer Life Technologies normalized to a concentration of 2.3nM and multiplexed. Prior to sequencing wefurther evaluated the quality of the multiplexed library by quantitative real timePCR using the Kapa Library Quantification Kit Kapa Biosystems. Two lanes ofpaired end 150 base pair bp sequencing were performed on an Illumina HiSeq 4000.         21dpf nr5a1a nr5a1b   strain:AB|age:21 dpf|sex:not applicable|tissue:trunk|biomaterial provider:Postlethwait lab University of Oregon|genotype:nr5a1a / ; nr5a1b / |BioSampleModel:Model organism or animal                 RNA Seq of Danio rerio: 21dpf nr5a1a nr5a1b double mutant 4 21dpf nr5a1a nr5a1b double mutant 4 21dpf nr5a1a nr5a1b double mutant 4 Strand specific RNA seq library prepared with BIOO NEXTflex Rapid Directional qRNA Seq kit     RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina HiSeq 4000   SRP219014     nr5a1ab21dmut-4_combined_R1.fq.gz nr5a1ab21dmut-4_combined_R2.fq.gz fastq fastq 4174074276.0 13821438.0 nr5a1ab21dmut 4 combined R1.fq.gz 0:151 1:151 A:1056833741;C:976094817;G:994472897;T:1145958470;N:714351 151 151     1056833741 976094817 994472897 1145958470 714351 SRX6748584 SRS5298723 SRA946180 University of Oregon|Institute of Neuroscience University of Oregon 2 0.89035 0.89997 0.10027 0.07415 0.7264 0.72829 0.48572 0.57325 151 151 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   United States 2019-08-21 Larval Larval Trunk Surface Structure
53674 53674 SRR10010355 SRX6748583 SRS5298723 SRP219014 PRJNA561212 RNA Seq of nr5a1a and nr5a1b mutant zebrafish PRJNA561212 Transcriptome Analysis Zebrafish were euthanized in Tricaine. We isolated gonad containing trunk of theanimals by removing the anterior of the fish from just posterior of the pectoral finand removing the caudal peduncle posterior to the anus. Trunks were individuallyhomogenized in 200ul Trizol. Total RNA was extracted using the Ribopure RNAPurification Kit ThermoFisher. Total RNA was enriched for mRNA using DynabeadsrOligodt25 ThermoFisher. We constructed indexed strand specific cDNA sequencinglibraries using the NEXTflextm qRNA seq kit BIOO Scientific. Libraryconcentrations were quantified using a Qubitr fluorometer Life Technologies normalized to a concentration of 2.3nM and multiplexed. Prior to sequencing wefurther evaluated the quality of the multiplexed library by quantitative real timePCR using the Kapa Library Quantification Kit Kapa Biosystems. Two lanes ofpaired end 150 base pair bp sequencing were performed on an Illumina HiSeq 4000.         21dpf nr5a1a nr5a1b   strain:AB|age:21 dpf|sex:not applicable|tissue:trunk|biomaterial provider:Postlethwait lab University of Oregon|genotype:nr5a1a / ; nr5a1b / |BioSampleModel:Model organism or animal                 RNA Seq of Danio rerio: 21dpf nr5a1a nr5a1b double mutant 1 21dpf nr5a1a nr5a1b double mutant 1 21dpf nr5a1a nr5a1b double mutant 1 Strand specific RNA seq library prepared with BIOO NEXTflex Rapid Directional qRNA Seq kit     RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina HiSeq 4000   SRP219014     nr5a1ab21dmut-1_combined_R1.fq.gz nr5a1ab21dmut-1_combined_R2.fq.gz fastq fastq 2417465606.0 8004853.0 nr5a1ab21dmut 1 combined R1.fq.gz 0:151 1:151 A:581742121;C:593490135;G:604923946;T:636944229;N:365175 151 151     581742121 593490135 604923946 636944229 365175 SRX6748583 SRS5298723 SRA946180 University of Oregon|Institute of Neuroscience University of Oregon 2 0.91256 0.92768 0.16171 0.10389 0.7525 0.75645 0.41647 0.53108 151 151 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   United States 2019-08-21 Larval Larval Trunk Surface Structure
53675 53675 SRR10010356 SRX6748582 SRS5298723 SRP219014 PRJNA561212 RNA Seq of nr5a1a and nr5a1b mutant zebrafish PRJNA561212 Transcriptome Analysis Zebrafish were euthanized in Tricaine. We isolated gonad containing trunk of theanimals by removing the anterior of the fish from just posterior of the pectoral finand removing the caudal peduncle posterior to the anus. Trunks were individuallyhomogenized in 200ul Trizol. Total RNA was extracted using the Ribopure RNAPurification Kit ThermoFisher. Total RNA was enriched for mRNA using DynabeadsrOligodt25 ThermoFisher. We constructed indexed strand specific cDNA sequencinglibraries using the NEXTflextm qRNA seq kit BIOO Scientific. Libraryconcentrations were quantified using a Qubitr fluorometer Life Technologies normalized to a concentration of 2.3nM and multiplexed. Prior to sequencing wefurther evaluated the quality of the multiplexed library by quantitative real timePCR using the Kapa Library Quantification Kit Kapa Biosystems. Two lanes ofpaired end 150 base pair bp sequencing were performed on an Illumina HiSeq 4000.         21dpf nr5a1a nr5a1b   strain:AB|age:21 dpf|sex:not applicable|tissue:trunk|biomaterial provider:Postlethwait lab University of Oregon|genotype:nr5a1a / ; nr5a1b / |BioSampleModel:Model organism or animal                 RNA Seq of Danio rerio: 21dpf nr5a1a nr5a1b double mutant 2 21dpf nr5a1a nr5a1b double mutant 2 21dpf nr5a1a nr5a1b double mutant 2 Strand specific RNA seq library prepared with BIOO NEXTflex Rapid Directional qRNA Seq kit     RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina HiSeq 4000   SRP219014     nr5a1ab21dmut-2_combined_R1.fq.gz nr5a1ab21dmut-2_combined_R2.fq.gz fastq fastq 3285689634.0 10879767.0 nr5a1ab21dmut 2 combined R1.fq.gz 0:151 1:151 A:808126419;C:794634133;G:804858824;T:877512631;N:557627 151 151     808126419 794634133 804858824 877512631 557627 SRX6748582 SRS5298723 SRA946180 University of Oregon|Institute of Neuroscience University of Oregon 2 0.90624 0.91945 0.14324 0.09474 0.74556 0.74901 0.46293 0.60218 151 151 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   United States 2019-08-21 Larval Larval Trunk Surface Structure
53676 53676 SRR10010357 SRX6748581 SRS5298719 SRP219014 PRJNA561212 RNA Seq of nr5a1a and nr5a1b mutant zebrafish PRJNA561212 Transcriptome Analysis Zebrafish were euthanized in Tricaine. We isolated gonad containing trunk of theanimals by removing the anterior of the fish from just posterior of the pectoral finand removing the caudal peduncle posterior to the anus. Trunks were individuallyhomogenized in 200ul Trizol. Total RNA was extracted using the Ribopure RNAPurification Kit ThermoFisher. Total RNA was enriched for mRNA using DynabeadsrOligodt25 ThermoFisher. We constructed indexed strand specific cDNA sequencinglibraries using the NEXTflextm qRNA seq kit BIOO Scientific. Libraryconcentrations were quantified using a Qubitr fluorometer Life Technologies normalized to a concentration of 2.3nM and multiplexed. Prior to sequencing wefurther evaluated the quality of the multiplexed library by quantitative real timePCR using the Kapa Library Quantification Kit Kapa Biosystems. Two lanes ofpaired end 150 base pair bp sequencing were performed on an Illumina HiSeq 4000.         35dpf nr5a1a   strain:AB|age:35 dpf|sex:not applicable|tissue:trunk|biomaterial provider:Postlethwait lab University of Oregon|genotype:nr5a1a / ; nr5a1b +/+|BioSampleModel:Model organism or animal                 RNA Seq of Danio rerio: 35dpf nr5a1a mutant 7 35dpf nr5a1a mutant 7 35dpf nr5a1a mutant 7 Strand specific RNA seq library prepared with BIOO NEXTflex Rapid Directional qRNA Seq kit     RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina HiSeq 4000   SRP219014     nr5a1a35dmut-7_combined_R1.fq.gz nr5a1a35dmut-7_combined_R2.fq.gz fastq fastq 2969897294.0 9834097.0 nr5a1a35dmut 7 combined R1.fq.gz 0:151 1:151 A:752593200;C:695994377;G:707935979;T:812884925;N:488813 151 151     752593200 695994377 707935979 812884925 488813 SRX6748581 SRS5298719 SRA946180 University of Oregon|Institute of Neuroscience University of Oregon 2 0.89218 0.906 0.09342 0.07022 0.73795 0.73898 0.51281 0.5911 151 151 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   United States 2019-08-21 Juvenile Juvenile Trunk Surface Structure
53677 53677 SRR10010358 SRX6748580 SRS5298719 SRP219014 PRJNA561212 RNA Seq of nr5a1a and nr5a1b mutant zebrafish PRJNA561212 Transcriptome Analysis Zebrafish were euthanized in Tricaine. We isolated gonad containing trunk of theanimals by removing the anterior of the fish from just posterior of the pectoral finand removing the caudal peduncle posterior to the anus. Trunks were individuallyhomogenized in 200ul Trizol. Total RNA was extracted using the Ribopure RNAPurification Kit ThermoFisher. Total RNA was enriched for mRNA using DynabeadsrOligodt25 ThermoFisher. We constructed indexed strand specific cDNA sequencinglibraries using the NEXTflextm qRNA seq kit BIOO Scientific. Libraryconcentrations were quantified using a Qubitr fluorometer Life Technologies normalized to a concentration of 2.3nM and multiplexed. Prior to sequencing wefurther evaluated the quality of the multiplexed library by quantitative real timePCR using the Kapa Library Quantification Kit Kapa Biosystems. Two lanes ofpaired end 150 base pair bp sequencing were performed on an Illumina HiSeq 4000.         35dpf nr5a1a   strain:AB|age:35 dpf|sex:not applicable|tissue:trunk|biomaterial provider:Postlethwait lab University of Oregon|genotype:nr5a1a / ; nr5a1b +/+|BioSampleModel:Model organism or animal                 RNA Seq of Danio rerio: 35dpf nr5a1a mutant 8 35dpf nr5a1a mutant 8 35dpf nr5a1a mutant 8 Strand specific RNA seq library prepared with BIOO NEXTflex Rapid Directional qRNA Seq kit     RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina HiSeq 4000   SRP219014     nr5a1a35dmut-8_combined_R1.fq.gz nr5a1a35dmut-8_combined_R2.fq.gz fastq fastq 6225038118.0 20612709.0 nr5a1a35dmut 8 combined R1.fq.gz 0:151 1:151 A:1559098665;C:1505493891;G:1534176065;T:1625255981;N:1013516 151 151     1559098665 1505493891 1534176065 1625255981 1013516 SRX6748580 SRS5298719 SRA946180 University of Oregon|Institute of Neuroscience University of Oregon 2 0.90023 0.90282 0.09369 0.06592 0.73525 0.73854 0.50906 0.59368 151 151 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   United States 2019-08-21 Juvenile Juvenile Trunk Surface Structure
53678 53678 SRR10010359 SRX6748579 SRS5298721 SRP219014 PRJNA561212 RNA Seq of nr5a1a and nr5a1b mutant zebrafish PRJNA561212 Transcriptome Analysis Zebrafish were euthanized in Tricaine. We isolated gonad containing trunk of theanimals by removing the anterior of the fish from just posterior of the pectoral finand removing the caudal peduncle posterior to the anus. Trunks were individuallyhomogenized in 200ul Trizol. Total RNA was extracted using the Ribopure RNAPurification Kit ThermoFisher. Total RNA was enriched for mRNA using DynabeadsrOligodt25 ThermoFisher. We constructed indexed strand specific cDNA sequencinglibraries using the NEXTflextm qRNA seq kit BIOO Scientific. Libraryconcentrations were quantified using a Qubitr fluorometer Life Technologies normalized to a concentration of 2.3nM and multiplexed. Prior to sequencing wefurther evaluated the quality of the multiplexed library by quantitative real timePCR using the Kapa Library Quantification Kit Kapa Biosystems. Two lanes ofpaired end 150 base pair bp sequencing were performed on an Illumina HiSeq 4000.         35dpf wildtype   strain:AB|age:35 dpf|sex:not applicable|tissue:trunk|biomaterial provider:Postlethwait lab University of Oregon|genotype:nr5a1a +/+ ; nr5a1b +/+|BioSampleModel:Model organism or animal                 RNA Seq of Danio rerio: 35dpf wild type 2 35dpf wild type 2 35dpf wild type 2 Strand specific RNA seq library prepared with BIOO NEXTflex Rapid Directional qRNA Seq kit     RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina HiSeq 4000   SRP219014     nr5a1_35day_WT-2_combined_R1.fq.gz nr5a1_35day_WT-2_combined_R2.fq.gz fastq fastq 4142275186.0 13716143.0 nr5a1 35day WT 2 combined R1.fq.gz 0:151 1:151 A:1055251193;C:967398418;G:984879234;T:1134062187;N:684154 151 151     1055251193 967398418 984879234 1134062187 684154 SRX6748579 SRS5298721 SRA946180 University of Oregon|Institute of Neuroscience University of Oregon 2 0.89791 0.91014 0.09157 0.06806 0.73927 0.74067 0.515 0.58656 151 151 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   United States 2019-08-21 Juvenile Juvenile Trunk Surface Structure
53679 53679 SRR10010360 SRX6748578 SRS5298721 SRP219014 PRJNA561212 RNA Seq of nr5a1a and nr5a1b mutant zebrafish PRJNA561212 Transcriptome Analysis Zebrafish were euthanized in Tricaine. We isolated gonad containing trunk of theanimals by removing the anterior of the fish from just posterior of the pectoral finand removing the caudal peduncle posterior to the anus. Trunks were individuallyhomogenized in 200ul Trizol. Total RNA was extracted using the Ribopure RNAPurification Kit ThermoFisher. Total RNA was enriched for mRNA using DynabeadsrOligodt25 ThermoFisher. We constructed indexed strand specific cDNA sequencinglibraries using the NEXTflextm qRNA seq kit BIOO Scientific. Libraryconcentrations were quantified using a Qubitr fluorometer Life Technologies normalized to a concentration of 2.3nM and multiplexed. Prior to sequencing wefurther evaluated the quality of the multiplexed library by quantitative real timePCR using the Kapa Library Quantification Kit Kapa Biosystems. Two lanes ofpaired end 150 base pair bp sequencing were performed on an Illumina HiSeq 4000.         35dpf wildtype   strain:AB|age:35 dpf|sex:not applicable|tissue:trunk|biomaterial provider:Postlethwait lab University of Oregon|genotype:nr5a1a +/+ ; nr5a1b +/+|BioSampleModel:Model organism or animal                 RNA Seq of Danio rerio: 35dpf wild type 3 35dpf wild type 3 35dpf wild type 3 Strand specific RNA seq library prepared with BIOO NEXTflex Rapid Directional qRNA Seq kit     RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina HiSeq 4000   SRP219014     nr5a1_35day_WT-3_combined_R2.fq.gz nr5a1_35day_WT-3_combined_R1.fq.gz fastq fastq 2471376532.0 8183366.0 nr5a1 35day WT 3 combined R1.fq.gz 0:151 1:151 A:615088670;C:576161730;G:582945558;T:696743565;N:437009 151 151     615088670 576161730 582945558 696743565 437009 SRX6748578 SRS5298721 SRA946180 University of Oregon|Institute of Neuroscience University of Oregon 2 0.87753 0.90284 0.1111 0.07857 0.72563 0.72794 0.50927 0.59766 151 151 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   United States 2019-08-21 Juvenile Juvenile Trunk Surface Structure
53680 53680 SRR10010361 SRX6748577 SRS5298721 SRP219014 PRJNA561212 RNA Seq of nr5a1a and nr5a1b mutant zebrafish PRJNA561212 Transcriptome Analysis Zebrafish were euthanized in Tricaine. We isolated gonad containing trunk of theanimals by removing the anterior of the fish from just posterior of the pectoral finand removing the caudal peduncle posterior to the anus. Trunks were individuallyhomogenized in 200ul Trizol. Total RNA was extracted using the Ribopure RNAPurification Kit ThermoFisher. Total RNA was enriched for mRNA using DynabeadsrOligodt25 ThermoFisher. We constructed indexed strand specific cDNA sequencinglibraries using the NEXTflextm qRNA seq kit BIOO Scientific. Libraryconcentrations were quantified using a Qubitr fluorometer Life Technologies normalized to a concentration of 2.3nM and multiplexed. Prior to sequencing wefurther evaluated the quality of the multiplexed library by quantitative real timePCR using the Kapa Library Quantification Kit Kapa Biosystems. Two lanes ofpaired end 150 base pair bp sequencing were performed on an Illumina HiSeq 4000.         35dpf wildtype   strain:AB|age:35 dpf|sex:not applicable|tissue:trunk|biomaterial provider:Postlethwait lab University of Oregon|genotype:nr5a1a +/+ ; nr5a1b +/+|BioSampleModel:Model organism or animal                 RNA Seq of Danio rerio: 35dpf wild type 4 35dpf wild type 4 35dpf wild type 4 Strand specific RNA seq library prepared with BIOO NEXTflex Rapid Directional qRNA Seq kit     RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina HiSeq 4000   SRP219014     nr5a1_35day_WT-4_combined_R1.fq.gz nr5a1_35day_WT-4_combined_R2.fq.gz fastq fastq 2116363754.0 7007827.0 nr5a1 35day WT 4 combined R1.fq.gz 0:151 1:151 A:517886726;C:489788898;G:502421580;T:605882187;N:384363 151 151     517886726 489788898 502421580 605882187 384363 SRX6748577 SRS5298721 SRA946180 University of Oregon|Institute of Neuroscience University of Oregon 2 0.87446 0.91282 0.08961 0.06167 0.75325 0.75363 0.50444 0.58991 151 151 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   United States 2019-08-21 Juvenile Juvenile Trunk Surface Structure
53681 53681 SRR10010362 SRX6748576 SRS5298721 SRP219014 PRJNA561212 RNA Seq of nr5a1a and nr5a1b mutant zebrafish PRJNA561212 Transcriptome Analysis Zebrafish were euthanized in Tricaine. We isolated gonad containing trunk of theanimals by removing the anterior of the fish from just posterior of the pectoral finand removing the caudal peduncle posterior to the anus. Trunks were individuallyhomogenized in 200ul Trizol. Total RNA was extracted using the Ribopure RNAPurification Kit ThermoFisher. Total RNA was enriched for mRNA using DynabeadsrOligodt25 ThermoFisher. We constructed indexed strand specific cDNA sequencinglibraries using the NEXTflextm qRNA seq kit BIOO Scientific. Libraryconcentrations were quantified using a Qubitr fluorometer Life Technologies normalized to a concentration of 2.3nM and multiplexed. Prior to sequencing wefurther evaluated the quality of the multiplexed library by quantitative real timePCR using the Kapa Library Quantification Kit Kapa Biosystems. Two lanes ofpaired end 150 base pair bp sequencing were performed on an Illumina HiSeq 4000.         35dpf wildtype   strain:AB|age:35 dpf|sex:not applicable|tissue:trunk|biomaterial provider:Postlethwait lab University of Oregon|genotype:nr5a1a +/+ ; nr5a1b +/+|BioSampleModel:Model organism or animal                 RNA Seq of Danio rerio: 35dpf wild type 5 35dpf wild type 5 35dpf wild type 5 Strand specific RNA seq library prepared with BIOO NEXTflex Rapid Directional qRNA Seq kit     RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina HiSeq 4000   SRP219014     nr5a1_35day_WT-5_combined_R1.fq.gz nr5a1_35day_WT-5_combined_R2.fq.gz fastq fastq 2730512464.0 9041432.0 nr5a1 35day WT 5 combined R1.fq.gz 0:151 1:151 A:676487187;C:641297195;G:648646413;T:763575356;N:506313 151 151     676487187 641297195 648646413 763575356 506313 SRX6748576 SRS5298721 SRA946180 University of Oregon|Institute of Neuroscience University of Oregon 2 0.87293 0.89776 0.08726 0.06177 0.7727 0.77402 0.47624 0.58994 151 151 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   United States 2019-08-21 Juvenile Juvenile Trunk Surface Structure
53682 53682 SRR10010363 SRX6748575 SRS5298721 SRP219014 PRJNA561212 RNA Seq of nr5a1a and nr5a1b mutant zebrafish PRJNA561212 Transcriptome Analysis Zebrafish were euthanized in Tricaine. We isolated gonad containing trunk of theanimals by removing the anterior of the fish from just posterior of the pectoral finand removing the caudal peduncle posterior to the anus. Trunks were individuallyhomogenized in 200ul Trizol. Total RNA was extracted using the Ribopure RNAPurification Kit ThermoFisher. Total RNA was enriched for mRNA using DynabeadsrOligodt25 ThermoFisher. We constructed indexed strand specific cDNA sequencinglibraries using the NEXTflextm qRNA seq kit BIOO Scientific. Libraryconcentrations were quantified using a Qubitr fluorometer Life Technologies normalized to a concentration of 2.3nM and multiplexed. Prior to sequencing wefurther evaluated the quality of the multiplexed library by quantitative real timePCR using the Kapa Library Quantification Kit Kapa Biosystems. Two lanes ofpaired end 150 base pair bp sequencing were performed on an Illumina HiSeq 4000.         35dpf wildtype   strain:AB|age:35 dpf|sex:not applicable|tissue:trunk|biomaterial provider:Postlethwait lab University of Oregon|genotype:nr5a1a +/+ ; nr5a1b +/+|BioSampleModel:Model organism or animal                 RNA Seq of Danio rerio: 35dpf wild type 6 35dpf wild type 6 35dpf wild type 6 Strand specific RNA seq library prepared with BIOO NEXTflex Rapid Directional qRNA Seq kit     RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina HiSeq 4000   SRP219014     nr5a1_35day_WT-6_combined_R1.fq.gz nr5a1_35day_WT-6_combined_R2.fq.gz fastq fastq 2539526758.0 8409029.0 nr5a1 35day WT 6 combined R1.fq.gz 0:151 1:151 A:654061696;C:592950317;G:606528343;T:685572585;N:413817 151 151     654061696 592950317 606528343 685572585 413817 SRX6748575 SRS5298721 SRA946180 University of Oregon|Institute of Neuroscience University of Oregon 2 0.88978 0.89645 0.09493 0.07075 0.73509 0.73965 0.53187 0.55068 151 151 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   United States 2019-08-21 Juvenile Juvenile Trunk Surface Structure
53683 53683 SRR10010364 SRX6748574 SRS5298721 SRP219014 PRJNA561212 RNA Seq of nr5a1a and nr5a1b mutant zebrafish PRJNA561212 Transcriptome Analysis Zebrafish were euthanized in Tricaine. We isolated gonad containing trunk of theanimals by removing the anterior of the fish from just posterior of the pectoral finand removing the caudal peduncle posterior to the anus. Trunks were individuallyhomogenized in 200ul Trizol. Total RNA was extracted using the Ribopure RNAPurification Kit ThermoFisher. Total RNA was enriched for mRNA using DynabeadsrOligodt25 ThermoFisher. We constructed indexed strand specific cDNA sequencinglibraries using the NEXTflextm qRNA seq kit BIOO Scientific. Libraryconcentrations were quantified using a Qubitr fluorometer Life Technologies normalized to a concentration of 2.3nM and multiplexed. Prior to sequencing wefurther evaluated the quality of the multiplexed library by quantitative real timePCR using the Kapa Library Quantification Kit Kapa Biosystems. Two lanes ofpaired end 150 base pair bp sequencing were performed on an Illumina HiSeq 4000.         35dpf wildtype   strain:AB|age:35 dpf|sex:not applicable|tissue:trunk|biomaterial provider:Postlethwait lab University of Oregon|genotype:nr5a1a +/+ ; nr5a1b +/+|BioSampleModel:Model organism or animal                 RNA Seq of Danio rerio: 35dpf wild type 9 35dpf wild type 9 35dpf wild type 9 Strand specific RNA seq library prepared with BIOO NEXTflex Rapid Directional qRNA Seq kit     RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina HiSeq 4000   SRP219014     nr5a1_35day_WT-9_combined_R1.fq.gz nr5a1_35day_WT-9_combined_R2.fq.gz fastq fastq 2585156844.0 8560122.0 nr5a1 35day WT 9 combined R1.fq.gz 0:151 1:151 A:659256640;C:603793032;G:616048994;T:705643382;N:414796 151 151     659256640 603793032 616048994 705643382 414796 SRX6748574 SRS5298721 SRA946180 University of Oregon|Institute of Neuroscience University of Oregon 2 0.90219 0.91318 0.11237 0.08413 0.74472 0.74799 0.52565 0.61427 151 151 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   United States 2019-08-21 Juvenile Juvenile Trunk Surface Structure
53684 53684 SRR10010365 SRX6748573 SRS5298718 SRP219014 PRJNA561212 RNA Seq of nr5a1a and nr5a1b mutant zebrafish PRJNA561212 Transcriptome Analysis Zebrafish were euthanized in Tricaine. We isolated gonad containing trunk of theanimals by removing the anterior of the fish from just posterior of the pectoral finand removing the caudal peduncle posterior to the anus. Trunks were individuallyhomogenized in 200ul Trizol. Total RNA was extracted using the Ribopure RNAPurification Kit ThermoFisher. Total RNA was enriched for mRNA using DynabeadsrOligodt25 ThermoFisher. We constructed indexed strand specific cDNA sequencinglibraries using the NEXTflextm qRNA seq kit BIOO Scientific. Libraryconcentrations were quantified using a Qubitr fluorometer Life Technologies normalized to a concentration of 2.3nM and multiplexed. Prior to sequencing wefurther evaluated the quality of the multiplexed library by quantitative real timePCR using the Kapa Library Quantification Kit Kapa Biosystems. Two lanes ofpaired end 150 base pair bp sequencing were performed on an Illumina HiSeq 4000.         21dpf nr5a1a   strain:AB|age:21 dpf|sex:not applicable|tissue:trunk|biomaterial provider:Postlethwait lab University of Oregon|genotype:nr5a1a / ; nr5a1b +/+|BioSampleModel:Model organism or animal                 RNA Seq of Danio rerio: 21dpf nr5a1a mutant 1 21dpf nr5a1a mutant 1 21dpf nr5a1a mutant 1 Strand specific RNA seq library prepared with BIOO NEXTflex Rapid Directional qRNA Seq kit     RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina HiSeq 4000   SRP219014     nr5a1a21dmut-1_combined_R1.fq.gz nr5a1a21dmut-1_combined_R2.fq.gz fastq fastq 4068155326.0 13470713.0 nr5a1a21dmut 1 combined R1.fq.gz 0:151 1:151 A:1017223225;C:976375957;G:994219587;T:1079701810;N:634747 151 151     1017223225 976375957 994219587 1079701810 634747 SRX6748573 SRS5298718 SRA946180 University of Oregon|Institute of Neuroscience University of Oregon 2 0.9093 0.91636 0.11458 0.0805 0.74592 0.74767 0.47751 0.56844 151 151 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   United States 2019-08-21 Larval Larval Trunk Surface Structure
53685 53685 SRR10010366 SRX6748572 SRS5298718 SRP219014 PRJNA561212 RNA Seq of nr5a1a and nr5a1b mutant zebrafish PRJNA561212 Transcriptome Analysis Zebrafish were euthanized in Tricaine. We isolated gonad containing trunk of theanimals by removing the anterior of the fish from just posterior of the pectoral finand removing the caudal peduncle posterior to the anus. Trunks were individuallyhomogenized in 200ul Trizol. Total RNA was extracted using the Ribopure RNAPurification Kit ThermoFisher. Total RNA was enriched for mRNA using DynabeadsrOligodt25 ThermoFisher. We constructed indexed strand specific cDNA sequencinglibraries using the NEXTflextm qRNA seq kit BIOO Scientific. Libraryconcentrations were quantified using a Qubitr fluorometer Life Technologies normalized to a concentration of 2.3nM and multiplexed. Prior to sequencing wefurther evaluated the quality of the multiplexed library by quantitative real timePCR using the Kapa Library Quantification Kit Kapa Biosystems. Two lanes ofpaired end 150 base pair bp sequencing were performed on an Illumina HiSeq 4000.         21dpf nr5a1a   strain:AB|age:21 dpf|sex:not applicable|tissue:trunk|biomaterial provider:Postlethwait lab University of Oregon|genotype:nr5a1a / ; nr5a1b +/+|BioSampleModel:Model organism or animal                 RNA Seq of Danio rerio: 21dpf nr5a1a mutant 2 21dpf nr5a1a mutant 2 21dpf nr5a1a mutant 2 Strand specific RNA seq library prepared with BIOO NEXTflex Rapid Directional qRNA Seq kit     RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina HiSeq 4000   SRP219014     nr5a1a21dmut-2_combined_R1.fq.gz nr5a1a21dmut-2_combined_R2.fq.gz fastq fastq 2505906608.0 8297704.0 nr5a1a21dmut 2 combined R1.fq.gz 0:151 1:151 A:628340326;C:582556533;G:591788311;T:702775119;N:446319 151 151     628340326 582556533 591788311 702775119 446319 SRX6748572 SRS5298718 SRA946180 University of Oregon|Institute of Neuroscience University of Oregon 2 0.86347 0.88795 0.12164 0.08953 0.73395 0.73419 0.46039 0.56191 151 151 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   United States 2019-08-21 Larval Larval Trunk Surface Structure
53686 53686 SRR10010367 SRX6748571 SRS5298718 SRP219014 PRJNA561212 RNA Seq of nr5a1a and nr5a1b mutant zebrafish PRJNA561212 Transcriptome Analysis Zebrafish were euthanized in Tricaine. We isolated gonad containing trunk of theanimals by removing the anterior of the fish from just posterior of the pectoral finand removing the caudal peduncle posterior to the anus. Trunks were individuallyhomogenized in 200ul Trizol. Total RNA was extracted using the Ribopure RNAPurification Kit ThermoFisher. Total RNA was enriched for mRNA using DynabeadsrOligodt25 ThermoFisher. We constructed indexed strand specific cDNA sequencinglibraries using the NEXTflextm qRNA seq kit BIOO Scientific. Libraryconcentrations were quantified using a Qubitr fluorometer Life Technologies normalized to a concentration of 2.3nM and multiplexed. Prior to sequencing wefurther evaluated the quality of the multiplexed library by quantitative real timePCR using the Kapa Library Quantification Kit Kapa Biosystems. Two lanes ofpaired end 150 base pair bp sequencing were performed on an Illumina HiSeq 4000.         21dpf nr5a1a   strain:AB|age:21 dpf|sex:not applicable|tissue:trunk|biomaterial provider:Postlethwait lab University of Oregon|genotype:nr5a1a / ; nr5a1b +/+|BioSampleModel:Model organism or animal                 RNA Seq of Danio rerio: 21dpf nr5a1a mutant 3 21dpf nr5a1a mutant 3 21dpf nr5a1a mutant 3 Strand specific RNA seq library prepared with BIOO NEXTflex Rapid Directional qRNA Seq kit     RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina HiSeq 4000   SRP219014     nr5a1a21dmut-3_combined_R1.fq.gz nr5a1a21dmut-3_combined_R2.fq.gz fastq fastq 5177865500.0 17145250.0 nr5a1a21dmut 3 combined R1.fq.gz 0:151 1:151 A:1314291498;C:1203926598;G:1215376030;T:1443474497;N:796877 151 151     1314291498 1203926598 1215376030 1443474497 796877 SRX6748571 SRS5298718 SRA946180 University of Oregon|Institute of Neuroscience University of Oregon 2 0.88608 0.89825 0.10844 0.08157 0.74744 0.75073 0.49195 0.55116 151 151 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   United States 2019-08-21 Larval Larval Trunk Surface Structure
53687 53687 SRR10010368 SRX6748570 SRS5298718 SRP219014 PRJNA561212 RNA Seq of nr5a1a and nr5a1b mutant zebrafish PRJNA561212 Transcriptome Analysis Zebrafish were euthanized in Tricaine. We isolated gonad containing trunk of theanimals by removing the anterior of the fish from just posterior of the pectoral finand removing the caudal peduncle posterior to the anus. Trunks were individuallyhomogenized in 200ul Trizol. Total RNA was extracted using the Ribopure RNAPurification Kit ThermoFisher. Total RNA was enriched for mRNA using DynabeadsrOligodt25 ThermoFisher. We constructed indexed strand specific cDNA sequencinglibraries using the NEXTflextm qRNA seq kit BIOO Scientific. Libraryconcentrations were quantified using a Qubitr fluorometer Life Technologies normalized to a concentration of 2.3nM and multiplexed. Prior to sequencing wefurther evaluated the quality of the multiplexed library by quantitative real timePCR using the Kapa Library Quantification Kit Kapa Biosystems. Two lanes ofpaired end 150 base pair bp sequencing were performed on an Illumina HiSeq 4000.         21dpf nr5a1a   strain:AB|age:21 dpf|sex:not applicable|tissue:trunk|biomaterial provider:Postlethwait lab University of Oregon|genotype:nr5a1a / ; nr5a1b +/+|BioSampleModel:Model organism or animal                 RNA Seq of Danio rerio: 21dpf nr5a1a mutant 4 21dpf nr5a1a mutant 4 21dpf nr5a1a mutant 4 Strand specific RNA seq library prepared with BIOO NEXTflex Rapid Directional qRNA Seq kit     RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina HiSeq 4000   SRP219014     nr5a1a21dmut-4_combined_R1.fq.gz nr5a1a21dmut-4_combined_R2.fq.gz fastq fastq 2110370866.0 6987983.0 nr5a1a21dmut 4 combined R1.fq.gz 0:151 1:151 A:522374283;C:482991859;G:494663937;T:609971148;N:369639 151 151     522374283 482991859 494663937 609971148 369639 SRX6748570 SRS5298718 SRA946180 University of Oregon|Institute of Neuroscience University of Oregon 2 0.86441 0.90249 0.09677 0.0767 0.7401 0.74046 0.43194 0.54853 151 151 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   United States 2019-08-21 Larval Larval Trunk Surface Structure
53688 53688 SRR10010369 SRX6748569 SRS5298716 SRP219014 PRJNA561212 RNA Seq of nr5a1a and nr5a1b mutant zebrafish PRJNA561212 Transcriptome Analysis Zebrafish were euthanized in Tricaine. We isolated gonad containing trunk of theanimals by removing the anterior of the fish from just posterior of the pectoral finand removing the caudal peduncle posterior to the anus. Trunks were individuallyhomogenized in 200ul Trizol. Total RNA was extracted using the Ribopure RNAPurification Kit ThermoFisher. Total RNA was enriched for mRNA using DynabeadsrOligodt25 ThermoFisher. We constructed indexed strand specific cDNA sequencinglibraries using the NEXTflextm qRNA seq kit BIOO Scientific. Libraryconcentrations were quantified using a Qubitr fluorometer Life Technologies normalized to a concentration of 2.3nM and multiplexed. Prior to sequencing wefurther evaluated the quality of the multiplexed library by quantitative real timePCR using the Kapa Library Quantification Kit Kapa Biosystems. Two lanes ofpaired end 150 base pair bp sequencing were performed on an Illumina HiSeq 4000.         35dpf nr5a1b   strain:AB|age:35 dpf|sex:not applicable|tissue:trunk|biomaterial provider:Postlethwait lab University of Oregon|genotype:nr5a1a +/+ ; nr5a1b / |BioSampleModel:Model organism or animal                 RNA Seq of Danio rerio: 35dpf nr5a1b mutant 8 35dpf nr5a1b mutant 8 35dpf nr5a1b mutant 8 Strand specific RNA seq library prepared with BIOO NEXTflex Rapid Directional qRNA Seq kit     RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina HiSeq 4000   SRP219014     nr5a1b35dmut-8_combined_R1.fq.gz nr5a1b35dmut-8_combined_R2.fq.gz fastq fastq 3225498920.0 10680460.0 nr5a1b35dmut 8 combined R1.fq.gz 0:151 1:151 A:810589172;C:772659409;G:785398777;T:856326001;N:525561 151 151     810589172 772659409 785398777 856326001 525561 SRX6748569 SRS5298716 SRA946180 University of Oregon|Institute of Neuroscience University of Oregon 2 0.9048 0.9133 0.0825 0.05993 0.74627 0.74933 0.52212 0.58896 151 151 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   United States 2019-08-21 Juvenile Juvenile Trunk Surface Structure
53689 53689 SRR10010370 SRX6748568 SRS5298720 SRP219014 PRJNA561212 RNA Seq of nr5a1a and nr5a1b mutant zebrafish PRJNA561212 Transcriptome Analysis Zebrafish were euthanized in Tricaine. We isolated gonad containing trunk of theanimals by removing the anterior of the fish from just posterior of the pectoral finand removing the caudal peduncle posterior to the anus. Trunks were individuallyhomogenized in 200ul Trizol. Total RNA was extracted using the Ribopure RNAPurification Kit ThermoFisher. Total RNA was enriched for mRNA using DynabeadsrOligodt25 ThermoFisher. We constructed indexed strand specific cDNA sequencinglibraries using the NEXTflextm qRNA seq kit BIOO Scientific. Libraryconcentrations were quantified using a Qubitr fluorometer Life Technologies normalized to a concentration of 2.3nM and multiplexed. Prior to sequencing wefurther evaluated the quality of the multiplexed library by quantitative real timePCR using the Kapa Library Quantification Kit Kapa Biosystems. Two lanes ofpaired end 150 base pair bp sequencing were performed on an Illumina HiSeq 4000.         21dpf nr5a1b   strain:AB|age:21 dpf|sex:not applicable|tissue:trunk|biomaterial provider:Postlethwait lab University of Oregon|genotype:nr5a1a +/+ ; nr5a1b / |BioSampleModel:Model organism or animal                 RNA Seq of Danio rerio: 21dpf nr5a1b mutant 5 21dpf nr5a1b mutant 5 21dpf nr5a1b mutant 5 Strand specific RNA seq library prepared with BIOO NEXTflex Rapid Directional qRNA Seq kit     RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina HiSeq 4000   SRP219014     nr5a1b21dmut-5_combined_R1.fq.gz nr5a1b21dmut-5_combined_R2.fq.gz fastq fastq 2397758898.0 7939599.0 nr5a1b21dmut 5 combined R1.fq.gz 0:151 1:151 A:615206092;C:551215873;G:563698041;T:667222159;N:416733 151 151     615206092 551215873 563698041 667222159 416733 SRX6748568 SRS5298720 SRA946180 University of Oregon|Institute of Neuroscience University of Oregon 2 0.85518 0.87383 0.07575 0.062 0.7433 0.74302 0.48889 0.54776 151 151 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   United States 2019-08-21 Larval Larval Trunk Surface Structure
53690 53690 SRR10010371 SRX6748567 SRS5298722 SRP219014 PRJNA561212 RNA Seq of nr5a1a and nr5a1b mutant zebrafish PRJNA561212 Transcriptome Analysis Zebrafish were euthanized in Tricaine. We isolated gonad containing trunk of theanimals by removing the anterior of the fish from just posterior of the pectoral finand removing the caudal peduncle posterior to the anus. Trunks were individuallyhomogenized in 200ul Trizol. Total RNA was extracted using the Ribopure RNAPurification Kit ThermoFisher. Total RNA was enriched for mRNA using DynabeadsrOligodt25 ThermoFisher. We constructed indexed strand specific cDNA sequencinglibraries using the NEXTflextm qRNA seq kit BIOO Scientific. Libraryconcentrations were quantified using a Qubitr fluorometer Life Technologies normalized to a concentration of 2.3nM and multiplexed. Prior to sequencing wefurther evaluated the quality of the multiplexed library by quantitative real timePCR using the Kapa Library Quantification Kit Kapa Biosystems. Two lanes ofpaired end 150 base pair bp sequencing were performed on an Illumina HiSeq 4000.         21dpf wildtype   strain:AB|age:21 dpf|sex:not applicable|tissue:trunk|biomaterial provider:Postlethwait lab University of Oregon|genotype:nr5a1a +/+ ; nr5a1b +/+|BioSampleModel:Model organism or animal                 RNA Seq of Danio rerio: 21dpf wild type 5 21dpf wild type 5 21dpf wild type 5 Strand specific RNA seq library prepared with BIOO NEXTflex Rapid Directional qRNA Seq kit     RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina HiSeq 4000   SRP219014     nr5a1_21day_WT-5_combined_R1.fq.gz nr5a1_21day_WT-5_combined_R2.fq.gz fastq fastq 1875926152.0 6211676.0 nr5a1 21day WT 5 combined R1.fq.gz 0:151 1:151 A:477701808;C:436167533;G:445970379;T:515756259;N:330173 151 151     477701808 436167533 445970379 515756259 330173 SRX6748567 SRS5298722 SRA946180 University of Oregon|Institute of Neuroscience University of Oregon 2 0.86641 0.88274 0.07822 0.06302 0.74065 0.74268 0.49044 0.53592 151 151 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   United States 2019-08-21 Larval Larval Trunk Surface Structure
53691 53691 SRR10010372 SRX6748566 SRS5298722 SRP219014 PRJNA561212 RNA Seq of nr5a1a and nr5a1b mutant zebrafish PRJNA561212 Transcriptome Analysis Zebrafish were euthanized in Tricaine. We isolated gonad containing trunk of theanimals by removing the anterior of the fish from just posterior of the pectoral finand removing the caudal peduncle posterior to the anus. Trunks were individuallyhomogenized in 200ul Trizol. Total RNA was extracted using the Ribopure RNAPurification Kit ThermoFisher. Total RNA was enriched for mRNA using DynabeadsrOligodt25 ThermoFisher. We constructed indexed strand specific cDNA sequencinglibraries using the NEXTflextm qRNA seq kit BIOO Scientific. Libraryconcentrations were quantified using a Qubitr fluorometer Life Technologies normalized to a concentration of 2.3nM and multiplexed. Prior to sequencing wefurther evaluated the quality of the multiplexed library by quantitative real timePCR using the Kapa Library Quantification Kit Kapa Biosystems. Two lanes ofpaired end 150 base pair bp sequencing were performed on an Illumina HiSeq 4000.         21dpf wildtype   strain:AB|age:21 dpf|sex:not applicable|tissue:trunk|biomaterial provider:Postlethwait lab University of Oregon|genotype:nr5a1a +/+ ; nr5a1b +/+|BioSampleModel:Model organism or animal                 RNA Seq of Danio rerio: 21dpf wild type 6 21dpf wild type 6 21dpf wild type 6 Strand specific RNA seq library prepared with BIOO NEXTflex Rapid Directional qRNA Seq kit     RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina HiSeq 4000   SRP219014     nr5a1_21day_WT-6_combined_R1.fq.gz nr5a1_21day_WT-6_combined_R2.fq.gz fastq fastq 3892257238.0 12888269.0 nr5a1 21day WT 6 combined R1.fq.gz 0:151 1:151 A:977107658;C:920939016;G:939376756;T:1054183786;N:650022 151 151     977107658 920939016 939376756 1054183786 650022 SRX6748566 SRS5298722 SRA946180 University of Oregon|Institute of Neuroscience University of Oregon 2 0.90581 0.91407 0.09238 0.06994 0.72928 0.7316 0.47464 0.54022 151 151 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   United States 2019-08-21 Larval Larval Trunk Surface Structure
53692 53692 SRR10010373 SRX6748565 SRS5298722 SRP219014 PRJNA561212 RNA Seq of nr5a1a and nr5a1b mutant zebrafish PRJNA561212 Transcriptome Analysis Zebrafish were euthanized in Tricaine. We isolated gonad containing trunk of theanimals by removing the anterior of the fish from just posterior of the pectoral finand removing the caudal peduncle posterior to the anus. Trunks were individuallyhomogenized in 200ul Trizol. Total RNA was extracted using the Ribopure RNAPurification Kit ThermoFisher. Total RNA was enriched for mRNA using DynabeadsrOligodt25 ThermoFisher. We constructed indexed strand specific cDNA sequencinglibraries using the NEXTflextm qRNA seq kit BIOO Scientific. Libraryconcentrations were quantified using a Qubitr fluorometer Life Technologies normalized to a concentration of 2.3nM and multiplexed. Prior to sequencing wefurther evaluated the quality of the multiplexed library by quantitative real timePCR using the Kapa Library Quantification Kit Kapa Biosystems. Two lanes ofpaired end 150 base pair bp sequencing were performed on an Illumina HiSeq 4000.         21dpf wildtype   strain:AB|age:21 dpf|sex:not applicable|tissue:trunk|biomaterial provider:Postlethwait lab University of Oregon|genotype:nr5a1a +/+ ; nr5a1b +/+|BioSampleModel:Model organism or animal                 RNA Seq of Danio rerio: 21dpf wild type 7 21dpf wild type 7 21dpf wild type 7 Strand specific RNA seq library prepared with BIOO NEXTflex Rapid Directional qRNA Seq kit     RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina HiSeq 4000   SRP219014     nr5a1_21day_WT-7_combined_R1.fq.gz nr5a1_21day_WT-7_combined_R2.fq.gz fastq fastq 2540194480.0 8411240.0 nr5a1 21day WT 7 combined R1.fq.gz 0:151 1:151 A:643088594;C:592170077;G:602584725;T:701928622;N:422462 151 151     643088594 592170077 602584725 701928622 422462 SRX6748565 SRS5298722 SRA946180 University of Oregon|Institute of Neuroscience University of Oregon 2 0.90208 0.91776 0.10234 0.07684 0.74381 0.74793 0.50627 0.5718 151 151 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   United States 2019-08-21 Larval Larval Trunk Surface Structure
53693 53693 SRR10010374 SRX6748564 SRS5298722 SRP219014 PRJNA561212 RNA Seq of nr5a1a and nr5a1b mutant zebrafish PRJNA561212 Transcriptome Analysis Zebrafish were euthanized in Tricaine. We isolated gonad containing trunk of theanimals by removing the anterior of the fish from just posterior of the pectoral finand removing the caudal peduncle posterior to the anus. Trunks were individuallyhomogenized in 200ul Trizol. Total RNA was extracted using the Ribopure RNAPurification Kit ThermoFisher. Total RNA was enriched for mRNA using DynabeadsrOligodt25 ThermoFisher. We constructed indexed strand specific cDNA sequencinglibraries using the NEXTflextm qRNA seq kit BIOO Scientific. Libraryconcentrations were quantified using a Qubitr fluorometer Life Technologies normalized to a concentration of 2.3nM and multiplexed. Prior to sequencing wefurther evaluated the quality of the multiplexed library by quantitative real timePCR using the Kapa Library Quantification Kit Kapa Biosystems. Two lanes ofpaired end 150 base pair bp sequencing were performed on an Illumina HiSeq 4000.         21dpf wildtype   strain:AB|age:21 dpf|sex:not applicable|tissue:trunk|biomaterial provider:Postlethwait lab University of Oregon|genotype:nr5a1a +/+ ; nr5a1b +/+|BioSampleModel:Model organism or animal                 RNA Seq of Danio rerio: 21dpf wild type 8 21dpf wild type 8 21dpf wild type 8 Strand specific RNA seq library prepared with BIOO NEXTflex Rapid Directional qRNA Seq kit     RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina HiSeq 4000   SRP219014     nr5a1_21day_WT-8_combined_R1.fq.gz nr5a1_21day_WT-8_combined_R2.fq.gz fastq fastq 3311633548.0 10965674.0 nr5a1 21day WT 8 combined R1.fq.gz 0:151 1:151 A:823322153;C:788459107;G:803264255;T:896048885;N:539148 151 151     823322153 788459107 803264255 896048885 539148 SRX6748564 SRS5298722 SRA946180 University of Oregon|Institute of Neuroscience University of Oregon 2 0.89814 0.91328 0.10771 0.07611 0.74456 0.74736 0.46236 0.55982 151 151 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   United States 2019-08-21 Larval Larval Trunk Surface Structure
53694 53694 SRR10010375 SRX6748563 SRS5298722 SRP219014 PRJNA561212 RNA Seq of nr5a1a and nr5a1b mutant zebrafish PRJNA561212 Transcriptome Analysis Zebrafish were euthanized in Tricaine. We isolated gonad containing trunk of theanimals by removing the anterior of the fish from just posterior of the pectoral finand removing the caudal peduncle posterior to the anus. Trunks were individuallyhomogenized in 200ul Trizol. Total RNA was extracted using the Ribopure RNAPurification Kit ThermoFisher. Total RNA was enriched for mRNA using DynabeadsrOligodt25 ThermoFisher. We constructed indexed strand specific cDNA sequencinglibraries using the NEXTflextm qRNA seq kit BIOO Scientific. Libraryconcentrations were quantified using a Qubitr fluorometer Life Technologies normalized to a concentration of 2.3nM and multiplexed. Prior to sequencing wefurther evaluated the quality of the multiplexed library by quantitative real timePCR using the Kapa Library Quantification Kit Kapa Biosystems. Two lanes ofpaired end 150 base pair bp sequencing were performed on an Illumina HiSeq 4000.         21dpf wildtype   strain:AB|age:21 dpf|sex:not applicable|tissue:trunk|biomaterial provider:Postlethwait lab University of Oregon|genotype:nr5a1a +/+ ; nr5a1b +/+|BioSampleModel:Model organism or animal                 RNA Seq of Danio rerio: 21dpf wild type 1 21dpf wild type 1 21dpf wild type 1 Strand specific RNA seq library prepared with BIOO NEXTflex Rapid Directional qRNA Seq kit     RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina HiSeq 4000   SRP219014     nr5a1_21day_WT-1_combined_R1.fq.gz nr5a1_21day_WT-1_combined_R2.fq.gz fastq fastq 4233284094.0 14017497.0 nr5a1 21day WT 1 combined R1.fq.gz 0:151 1:151 A:1019536917;C:1019670795;G:1048583808;T:1144823115;N:669459 151 151     1019536917 1019670795 1048583808 1144823115 669459 SRX6748563 SRS5298722 SRA946180 University of Oregon|Institute of Neuroscience University of Oregon 2 0.90465 0.92862 0.12949 0.08548 0.75759 0.76134 0.46256 0.54131 151 151 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   United States 2019-08-21 Larval Larval Trunk Surface Structure
53695 53695 SRR10010376 SRX6748562 SRS5298722 SRP219014 PRJNA561212 RNA Seq of nr5a1a and nr5a1b mutant zebrafish PRJNA561212 Transcriptome Analysis Zebrafish were euthanized in Tricaine. We isolated gonad containing trunk of theanimals by removing the anterior of the fish from just posterior of the pectoral finand removing the caudal peduncle posterior to the anus. Trunks were individuallyhomogenized in 200ul Trizol. Total RNA was extracted using the Ribopure RNAPurification Kit ThermoFisher. Total RNA was enriched for mRNA using DynabeadsrOligodt25 ThermoFisher. We constructed indexed strand specific cDNA sequencinglibraries using the NEXTflextm qRNA seq kit BIOO Scientific. Libraryconcentrations were quantified using a Qubitr fluorometer Life Technologies normalized to a concentration of 2.3nM and multiplexed. Prior to sequencing wefurther evaluated the quality of the multiplexed library by quantitative real timePCR using the Kapa Library Quantification Kit Kapa Biosystems. Two lanes ofpaired end 150 base pair bp sequencing were performed on an Illumina HiSeq 4000.         21dpf wildtype   strain:AB|age:21 dpf|sex:not applicable|tissue:trunk|biomaterial provider:Postlethwait lab University of Oregon|genotype:nr5a1a +/+ ; nr5a1b +/+|BioSampleModel:Model organism or animal                 RNA Seq of Danio rerio: 21dpf wild type 2 21dpf wild type 2 21dpf wild type 2 Strand specific RNA seq library prepared with BIOO NEXTflex Rapid Directional qRNA Seq kit     RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina HiSeq 4000   SRP219014     nr5a1_21day_WT-2_combined_R1.fq.gz nr5a1_21day_WT-2_combined_R2.fq.gz fastq fastq 3045409978.0 10084139.0 nr5a1 21day WT 2 combined R1.fq.gz 0:151 1:151 A:750366581;C:741452134;G:747756768;T:805335602;N:498893 151 151     750366581 741452134 747756768 805335602 498893 SRX6748562 SRS5298722 SRA946180 University of Oregon|Institute of Neuroscience University of Oregon 2 0.91038 0.91898 0.12816 0.0853 0.7475 0.75063 0.45891 0.5815 151 151 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   United States 2019-08-21 Larval Larval Trunk Surface Structure
53696 53696 SRR10010377 SRX6748561 SRS5298722 SRP219014 PRJNA561212 RNA Seq of nr5a1a and nr5a1b mutant zebrafish PRJNA561212 Transcriptome Analysis Zebrafish were euthanized in Tricaine. We isolated gonad containing trunk of theanimals by removing the anterior of the fish from just posterior of the pectoral finand removing the caudal peduncle posterior to the anus. Trunks were individuallyhomogenized in 200ul Trizol. Total RNA was extracted using the Ribopure RNAPurification Kit ThermoFisher. Total RNA was enriched for mRNA using DynabeadsrOligodt25 ThermoFisher. We constructed indexed strand specific cDNA sequencinglibraries using the NEXTflextm qRNA seq kit BIOO Scientific. Libraryconcentrations were quantified using a Qubitr fluorometer Life Technologies normalized to a concentration of 2.3nM and multiplexed. Prior to sequencing wefurther evaluated the quality of the multiplexed library by quantitative real timePCR using the Kapa Library Quantification Kit Kapa Biosystems. Two lanes ofpaired end 150 base pair bp sequencing were performed on an Illumina HiSeq 4000.         21dpf wildtype   strain:AB|age:21 dpf|sex:not applicable|tissue:trunk|biomaterial provider:Postlethwait lab University of Oregon|genotype:nr5a1a +/+ ; nr5a1b +/+|BioSampleModel:Model organism or animal                 RNA Seq of Danio rerio: 21dpf wild type 3 21dpf wild type 3 21dpf wild type 3 Strand specific RNA seq library prepared with BIOO NEXTflex Rapid Directional qRNA Seq kit     RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina HiSeq 4000   SRP219014     nr5a1_21day_WT-3_combined_R1.fq.gz nr5a1_21day_WT-3_combined_R2.fq.gz fastq fastq 2883771122.0 9548911.0 nr5a1 21day WT 3 combined R1.fq.gz 0:151 1:151 A:720580530;C:687030945;G:693106513;T:782554064;N:499070 151 151     720580530 687030945 693106513 782554064 499070 SRX6748561 SRS5298722 SRA946180 University of Oregon|Institute of Neuroscience University of Oregon 2 0.89664 0.90411 0.09857 0.07143 0.73742 0.73852 0.47183 0.55193 151 151 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   United States 2019-08-21 Larval Larval Trunk Surface Structure
53697 53697 SRR10010378 SRX6748560 SRS5298722 SRP219014 PRJNA561212 RNA Seq of nr5a1a and nr5a1b mutant zebrafish PRJNA561212 Transcriptome Analysis Zebrafish were euthanized in Tricaine. We isolated gonad containing trunk of theanimals by removing the anterior of the fish from just posterior of the pectoral finand removing the caudal peduncle posterior to the anus. Trunks were individuallyhomogenized in 200ul Trizol. Total RNA was extracted using the Ribopure RNAPurification Kit ThermoFisher. Total RNA was enriched for mRNA using DynabeadsrOligodt25 ThermoFisher. We constructed indexed strand specific cDNA sequencinglibraries using the NEXTflextm qRNA seq kit BIOO Scientific. Libraryconcentrations were quantified using a Qubitr fluorometer Life Technologies normalized to a concentration of 2.3nM and multiplexed. Prior to sequencing wefurther evaluated the quality of the multiplexed library by quantitative real timePCR using the Kapa Library Quantification Kit Kapa Biosystems. Two lanes ofpaired end 150 base pair bp sequencing were performed on an Illumina HiSeq 4000.         21dpf wildtype   strain:AB|age:21 dpf|sex:not applicable|tissue:trunk|biomaterial provider:Postlethwait lab University of Oregon|genotype:nr5a1a +/+ ; nr5a1b +/+|BioSampleModel:Model organism or animal                 RNA Seq of Danio rerio: 21dpf wild type 4 21dpf wild type 4 21dpf wild type 4 Strand specific RNA seq library prepared with BIOO NEXTflex Rapid Directional qRNA Seq kit     RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina HiSeq 4000   SRP219014     nr5a1_21day_WT-4_combined_R1.fq.gz nr5a1_21day_WT-4_combined_R2.fq.gz fastq fastq 1669634784.0 5528592.0 nr5a1 21day WT 4 combined R1.fq.gz 0:151 1:151 A:412697030;C:395937730;G:404509253;T:456214241;N:276530 151 151     412697030 395937730 404509253 456214241 276530 SRX6748560 SRS5298722 SRA946180 University of Oregon|Institute of Neuroscience University of Oregon 2 0.87728 0.89949 0.10088 0.07121 0.75383 0.75521 0.46951 0.57045 151 151 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   United States 2019-08-21 Larval Larval Trunk Surface Structure
53698 53698 SRR10010379 SRX6748559 SRS5298721 SRP219014 PRJNA561212 RNA Seq of nr5a1a and nr5a1b mutant zebrafish PRJNA561212 Transcriptome Analysis Zebrafish were euthanized in Tricaine. We isolated gonad containing trunk of theanimals by removing the anterior of the fish from just posterior of the pectoral finand removing the caudal peduncle posterior to the anus. Trunks were individuallyhomogenized in 200ul Trizol. Total RNA was extracted using the Ribopure RNAPurification Kit ThermoFisher. Total RNA was enriched for mRNA using DynabeadsrOligodt25 ThermoFisher. We constructed indexed strand specific cDNA sequencinglibraries using the NEXTflextm qRNA seq kit BIOO Scientific. Libraryconcentrations were quantified using a Qubitr fluorometer Life Technologies normalized to a concentration of 2.3nM and multiplexed. Prior to sequencing wefurther evaluated the quality of the multiplexed library by quantitative real timePCR using the Kapa Library Quantification Kit Kapa Biosystems. Two lanes ofpaired end 150 base pair bp sequencing were performed on an Illumina HiSeq 4000.         35dpf wildtype   strain:AB|age:35 dpf|sex:not applicable|tissue:trunk|biomaterial provider:Postlethwait lab University of Oregon|genotype:nr5a1a +/+ ; nr5a1b +/+|BioSampleModel:Model organism or animal                 RNA Seq of Danio rerio: 35dpf wild type 10 35dpf wild type 10 35dpf wild type 10 Strand specific RNA seq library prepared with BIOO NEXTflex Rapid Directional qRNA Seq kit     RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina HiSeq 4000   SRP219014     nr5a1_35day_WT-10_combined_R1.fq.gz nr5a1_35day_WT-10_combined_R2.fq.gz fastq fastq 3038724000.0 10062000.0 nr5a1 35day WT 10 combined R1.fq.gz 0:151 1:151 A:764571339;C:715912069;G:728730982;T:828987265;N:522345 151 151     764571339 715912069 728730982 828987265 522345 SRX6748559 SRS5298721 SRA946180 University of Oregon|Institute of Neuroscience University of Oregon 2 0.87838 0.89181 0.08908 0.06618 0.7484 0.7485 0.53115 0.59993 151 151 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   United States 2019-08-21 Juvenile Juvenile Trunk Surface Structure
53699 53699 SRR10010380 SRX6748558 SRS5298721 SRP219014 PRJNA561212 RNA Seq of nr5a1a and nr5a1b mutant zebrafish PRJNA561212 Transcriptome Analysis Zebrafish were euthanized in Tricaine. We isolated gonad containing trunk of theanimals by removing the anterior of the fish from just posterior of the pectoral finand removing the caudal peduncle posterior to the anus. Trunks were individuallyhomogenized in 200ul Trizol. Total RNA was extracted using the Ribopure RNAPurification Kit ThermoFisher. Total RNA was enriched for mRNA using DynabeadsrOligodt25 ThermoFisher. We constructed indexed strand specific cDNA sequencinglibraries using the NEXTflextm qRNA seq kit BIOO Scientific. Libraryconcentrations were quantified using a Qubitr fluorometer Life Technologies normalized to a concentration of 2.3nM and multiplexed. Prior to sequencing wefurther evaluated the quality of the multiplexed library by quantitative real timePCR using the Kapa Library Quantification Kit Kapa Biosystems. Two lanes ofpaired end 150 base pair bp sequencing were performed on an Illumina HiSeq 4000.         35dpf wildtype   strain:AB|age:35 dpf|sex:not applicable|tissue:trunk|biomaterial provider:Postlethwait lab University of Oregon|genotype:nr5a1a +/+ ; nr5a1b +/+|BioSampleModel:Model organism or animal                 RNA Seq of Danio rerio: 35dpf wild type 1 35dpf wild type 1 35dpf wild type 1 Strand specific RNA seq library prepared with BIOO NEXTflex Rapid Directional qRNA Seq kit     RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina HiSeq 4000   SRP219014     nr5a1_35day_WT-1_combined_R1.fq.gz nr5a1_35day_WT-1_combined_R2.fq.gz fastq fastq 3598780584.0 11916492.0 nr5a1 35day WT 1 combined R1.fq.gz 0:151 1:151 A:886857565;C:874550481;G:894991137;T:941808129;N:573272 151 151     886857565 874550481 894991137 941808129 573272 SRX6748558 SRS5298721 SRA946180 University of Oregon|Institute of Neuroscience University of Oregon 2 0.9139 0.92164 0.10525 0.06463 0.75057 0.75211 0.49642 0.57074 151 151 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   United States 2019-08-21 Juvenile Juvenile Trunk Surface Structure
53700 53700 SRR10010381 SRX6748557 SRS5298716 SRP219014 PRJNA561212 RNA Seq of nr5a1a and nr5a1b mutant zebrafish PRJNA561212 Transcriptome Analysis Zebrafish were euthanized in Tricaine. We isolated gonad containing trunk of theanimals by removing the anterior of the fish from just posterior of the pectoral finand removing the caudal peduncle posterior to the anus. Trunks were individuallyhomogenized in 200ul Trizol. Total RNA was extracted using the Ribopure RNAPurification Kit ThermoFisher. Total RNA was enriched for mRNA using DynabeadsrOligodt25 ThermoFisher. We constructed indexed strand specific cDNA sequencinglibraries using the NEXTflextm qRNA seq kit BIOO Scientific. Libraryconcentrations were quantified using a Qubitr fluorometer Life Technologies normalized to a concentration of 2.3nM and multiplexed. Prior to sequencing wefurther evaluated the quality of the multiplexed library by quantitative real timePCR using the Kapa Library Quantification Kit Kapa Biosystems. Two lanes ofpaired end 150 base pair bp sequencing were performed on an Illumina HiSeq 4000.         35dpf nr5a1b   strain:AB|age:35 dpf|sex:not applicable|tissue:trunk|biomaterial provider:Postlethwait lab University of Oregon|genotype:nr5a1a +/+ ; nr5a1b / |BioSampleModel:Model organism or animal                 RNA Seq of Danio rerio: 35dpf nr5a1b mutant 6 35dpf nr5a1b mutant 6 35dpf nr5a1b mutant 6 Strand specific RNA seq library prepared with BIOO NEXTflex Rapid Directional qRNA Seq kit     RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina HiSeq 4000   SRP219014     nr5a1b35dmut-6_combined_R1.fq.gz nr5a1b35dmut-6_combined_R2.fq.gz fastq fastq 4476352418.0 14822359.0 nr5a1b35dmut 6 combined R1.fq.gz 0:151 1:151 A:1120206233;C:1067519728;G:1089929887;T:1197971503;N:725067 151 151     1120206233 1067519728 1089929887 1197971503 725067 SRX6748557 SRS5298716 SRA946180 University of Oregon|Institute of Neuroscience University of Oregon 2 0.91501 0.91912 0.08509 0.05807 0.74663 0.7488 0.51898 0.57667 151 151 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   United States 2019-08-21 Juvenile Juvenile Trunk Surface Structure
53701 53701 SRR10010382 SRX6748556 SRS5298716 SRP219014 PRJNA561212 RNA Seq of nr5a1a and nr5a1b mutant zebrafish PRJNA561212 Transcriptome Analysis Zebrafish were euthanized in Tricaine. We isolated gonad containing trunk of theanimals by removing the anterior of the fish from just posterior of the pectoral finand removing the caudal peduncle posterior to the anus. Trunks were individuallyhomogenized in 200ul Trizol. Total RNA was extracted using the Ribopure RNAPurification Kit ThermoFisher. Total RNA was enriched for mRNA using DynabeadsrOligodt25 ThermoFisher. We constructed indexed strand specific cDNA sequencinglibraries using the NEXTflextm qRNA seq kit BIOO Scientific. Libraryconcentrations were quantified using a Qubitr fluorometer Life Technologies normalized to a concentration of 2.3nM and multiplexed. Prior to sequencing wefurther evaluated the quality of the multiplexed library by quantitative real timePCR using the Kapa Library Quantification Kit Kapa Biosystems. Two lanes ofpaired end 150 base pair bp sequencing were performed on an Illumina HiSeq 4000.         35dpf nr5a1b   strain:AB|age:35 dpf|sex:not applicable|tissue:trunk|biomaterial provider:Postlethwait lab University of Oregon|genotype:nr5a1a +/+ ; nr5a1b / |BioSampleModel:Model organism or animal                 RNA Seq of Danio rerio: 35dpf nr5a1b mutant 5 35dpf nr5a1b mutant 5 35dpf nr5a1b mutant 5 Strand specific RNA seq library prepared with BIOO NEXTflex Rapid Directional qRNA Seq kit     RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina HiSeq 4000   SRP219014     nr5a1b35dmut-5_combined_R1.fq.gz nr5a1b35dmut-5_combined_R2.fq.gz fastq fastq 4009328142.0 13275921.0 nr5a1b35dmut 5 combined R1.fq.gz 0:151 1:151 A:1067904920;C:901186174;G:923523205;T:1116013227;N:700616 151 151     1067904920 901186174 923523205 1116013227 700616 SRX6748556 SRS5298716 SRA946180 University of Oregon|Institute of Neuroscience University of Oregon 2 0.80761 0.83261 0.06793 0.05143 0.75911 0.76114 0.55479 0.61755 151 151 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   United States 2019-08-21 Juvenile Juvenile Trunk Surface Structure
53702 53702 SRR10010383 SRX6748555 SRS5298720 SRP219014 PRJNA561212 RNA Seq of nr5a1a and nr5a1b mutant zebrafish PRJNA561212 Transcriptome Analysis Zebrafish were euthanized in Tricaine. We isolated gonad containing trunk of theanimals by removing the anterior of the fish from just posterior of the pectoral finand removing the caudal peduncle posterior to the anus. Trunks were individuallyhomogenized in 200ul Trizol. Total RNA was extracted using the Ribopure RNAPurification Kit ThermoFisher. Total RNA was enriched for mRNA using DynabeadsrOligodt25 ThermoFisher. We constructed indexed strand specific cDNA sequencinglibraries using the NEXTflextm qRNA seq kit BIOO Scientific. Libraryconcentrations were quantified using a Qubitr fluorometer Life Technologies normalized to a concentration of 2.3nM and multiplexed. Prior to sequencing wefurther evaluated the quality of the multiplexed library by quantitative real timePCR using the Kapa Library Quantification Kit Kapa Biosystems. Two lanes ofpaired end 150 base pair bp sequencing were performed on an Illumina HiSeq 4000.         21dpf nr5a1b   strain:AB|age:21 dpf|sex:not applicable|tissue:trunk|biomaterial provider:Postlethwait lab University of Oregon|genotype:nr5a1a +/+ ; nr5a1b / |BioSampleModel:Model organism or animal                 RNA Seq of Danio rerio: 21dpf nr5a1b mutant 2 21dpf nr5a1b mutant 2 21dpf nr5a1b mutant 2 Strand specific RNA seq library prepared with BIOO NEXTflex Rapid Directional qRNA Seq kit     RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina HiSeq 4000   SRP219014     nr5a1b21dmut-2_combined_R1.fq.gz nr5a1b21dmut-2_combined_R2.fq.gz fastq fastq 2264787392.0 7499296.0 nr5a1b21dmut 2 combined R1.fq.gz 0:151 1:151 A:577188441;C:509507826;G:516653827;T:661021300;N:415998 151 151     577188441 509507826 516653827 661021300 415998 SRX6748555 SRS5298720 SRA946180 University of Oregon|Institute of Neuroscience University of Oregon 2 0.85013 0.88233 0.10196 0.07899 0.72762 0.72768 0.50451 0.57721 151 151 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   United States 2019-08-21 Larval Larval Trunk Surface Structure
53703 53703 SRR10010384 SRX6748554 SRS5298720 SRP219014 PRJNA561212 RNA Seq of nr5a1a and nr5a1b mutant zebrafish PRJNA561212 Transcriptome Analysis Zebrafish were euthanized in Tricaine. We isolated gonad containing trunk of theanimals by removing the anterior of the fish from just posterior of the pectoral finand removing the caudal peduncle posterior to the anus. Trunks were individuallyhomogenized in 200ul Trizol. Total RNA was extracted using the Ribopure RNAPurification Kit ThermoFisher. Total RNA was enriched for mRNA using DynabeadsrOligodt25 ThermoFisher. We constructed indexed strand specific cDNA sequencinglibraries using the NEXTflextm qRNA seq kit BIOO Scientific. Libraryconcentrations were quantified using a Qubitr fluorometer Life Technologies normalized to a concentration of 2.3nM and multiplexed. Prior to sequencing wefurther evaluated the quality of the multiplexed library by quantitative real timePCR using the Kapa Library Quantification Kit Kapa Biosystems. Two lanes ofpaired end 150 base pair bp sequencing were performed on an Illumina HiSeq 4000.         21dpf nr5a1b   strain:AB|age:21 dpf|sex:not applicable|tissue:trunk|biomaterial provider:Postlethwait lab University of Oregon|genotype:nr5a1a +/+ ; nr5a1b / |BioSampleModel:Model organism or animal                 RNA Seq of Danio rerio: 21dpf nr5a1b mutant 1 21dpf nr5a1b mutant 1 21dpf nr5a1b mutant 1 Strand specific RNA seq library prepared with BIOO NEXTflex Rapid Directional qRNA Seq kit     RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina HiSeq 4000   SRP219014     nr5a1b21dmut-1_combined_R1.fq.gz nr5a1b21dmut-1_combined_R2.fq.gz fastq fastq 5900631530.0 19538515.0 nr5a1b21dmut 1 combined R1.fq.gz 0:151 1:151 A:1457892768;C:1433320625;G:1464136662;T:1544328487;N:952988 151 151     1457892768 1433320625 1464136662 1544328487 952988 SRX6748554 SRS5298720 SRA946180 University of Oregon|Institute of Neuroscience University of Oregon 2 0.90637 0.91209 0.14421 0.09815 0.73673 0.73959 0.45526 0.58028 151 151 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   United States 2019-08-21 Larval Larval Trunk Surface Structure
53704 53704 SRR10010385 SRX6748553 SRS5298719 SRP219014 PRJNA561212 RNA Seq of nr5a1a and nr5a1b mutant zebrafish PRJNA561212 Transcriptome Analysis Zebrafish were euthanized in Tricaine. We isolated gonad containing trunk of theanimals by removing the anterior of the fish from just posterior of the pectoral finand removing the caudal peduncle posterior to the anus. Trunks were individuallyhomogenized in 200ul Trizol. Total RNA was extracted using the Ribopure RNAPurification Kit ThermoFisher. Total RNA was enriched for mRNA using DynabeadsrOligodt25 ThermoFisher. We constructed indexed strand specific cDNA sequencinglibraries using the NEXTflextm qRNA seq kit BIOO Scientific. Libraryconcentrations were quantified using a Qubitr fluorometer Life Technologies normalized to a concentration of 2.3nM and multiplexed. Prior to sequencing wefurther evaluated the quality of the multiplexed library by quantitative real timePCR using the Kapa Library Quantification Kit Kapa Biosystems. Two lanes ofpaired end 150 base pair bp sequencing were performed on an Illumina HiSeq 4000.         35dpf nr5a1a   strain:AB|age:35 dpf|sex:not applicable|tissue:trunk|biomaterial provider:Postlethwait lab University of Oregon|genotype:nr5a1a / ; nr5a1b +/+|BioSampleModel:Model organism or animal                 RNA Seq of Danio rerio: 35dpf nr5a1a mutant 6 35dpf nr5a1a mutant 6 35dpf nr5a1a mutant 6 Strand specific RNA seq library prepared with BIOO NEXTflex Rapid Directional qRNA Seq kit     RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina HiSeq 4000   SRP219014     nr5a1a35dmut-6_combined_R1.fq.gz nr5a1a35dmut-6_combined_R2.fq.gz fastq fastq 3073492354.0 10177127.0 nr5a1a35dmut 6 combined R1.fq.gz 0:151 1:151 A:789160217;C:716824009;G:740200723;T:826797684;N:509721 151 151     789160217 716824009 740200723 826797684 509721 SRX6748553 SRS5298719 SRA946180 University of Oregon|Institute of Neuroscience University of Oregon 2 0.87516 0.88431 0.09065 0.07016 0.74588 0.74726 0.53057 0.60951 151 151 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   United States 2019-08-21 Juvenile Juvenile Trunk Surface Structure
53705 53705 SRR10010386 SRX6748552 SRS5298719 SRP219014 PRJNA561212 RNA Seq of nr5a1a and nr5a1b mutant zebrafish PRJNA561212 Transcriptome Analysis Zebrafish were euthanized in Tricaine. We isolated gonad containing trunk of theanimals by removing the anterior of the fish from just posterior of the pectoral finand removing the caudal peduncle posterior to the anus. Trunks were individuallyhomogenized in 200ul Trizol. Total RNA was extracted using the Ribopure RNAPurification Kit ThermoFisher. Total RNA was enriched for mRNA using DynabeadsrOligodt25 ThermoFisher. We constructed indexed strand specific cDNA sequencinglibraries using the NEXTflextm qRNA seq kit BIOO Scientific. Libraryconcentrations were quantified using a Qubitr fluorometer Life Technologies normalized to a concentration of 2.3nM and multiplexed. Prior to sequencing wefurther evaluated the quality of the multiplexed library by quantitative real timePCR using the Kapa Library Quantification Kit Kapa Biosystems. Two lanes ofpaired end 150 base pair bp sequencing were performed on an Illumina HiSeq 4000.         35dpf nr5a1a   strain:AB|age:35 dpf|sex:not applicable|tissue:trunk|biomaterial provider:Postlethwait lab University of Oregon|genotype:nr5a1a / ; nr5a1b +/+|BioSampleModel:Model organism or animal                 RNA Seq of Danio rerio: 35dpf nr5a1a mutant 5 35dpf nr5a1a mutant 5 35dpf nr5a1a mutant 5 Strand specific RNA seq library prepared with BIOO NEXTflex Rapid Directional qRNA Seq kit     RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina HiSeq 4000   SRP219014     nr5a1a35dmut-5_combined_R1.fq.gz nr5a1a35dmut-5_combined_R2.fq.gz fastq fastq 3240379668.0 10729734.0 nr5a1a35dmut 5 combined R1.fq.gz 0:151 1:151 A:850546438;C:745212890;G:765395973;T:878658019;N:566348 151 151     850546438 745212890 765395973 878658019 566348 SRX6748552 SRS5298719 SRA946180 University of Oregon|Institute of Neuroscience University of Oregon 2 0.81872 0.83649 0.07435 0.05495 0.75225 0.75461 0.51257 0.58856 151 151 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   United States 2019-08-21 Juvenile Juvenile Trunk Surface Structure
53706 53706 SRR10010387 SRX6748551 SRS5298720 SRP219014 PRJNA561212 RNA Seq of nr5a1a and nr5a1b mutant zebrafish PRJNA561212 Transcriptome Analysis Zebrafish were euthanized in Tricaine. We isolated gonad containing trunk of theanimals by removing the anterior of the fish from just posterior of the pectoral finand removing the caudal peduncle posterior to the anus. Trunks were individuallyhomogenized in 200ul Trizol. Total RNA was extracted using the Ribopure RNAPurification Kit ThermoFisher. Total RNA was enriched for mRNA using DynabeadsrOligodt25 ThermoFisher. We constructed indexed strand specific cDNA sequencinglibraries using the NEXTflextm qRNA seq kit BIOO Scientific. Libraryconcentrations were quantified using a Qubitr fluorometer Life Technologies normalized to a concentration of 2.3nM and multiplexed. Prior to sequencing wefurther evaluated the quality of the multiplexed library by quantitative real timePCR using the Kapa Library Quantification Kit Kapa Biosystems. Two lanes ofpaired end 150 base pair bp sequencing were performed on an Illumina HiSeq 4000.         21dpf nr5a1b   strain:AB|age:21 dpf|sex:not applicable|tissue:trunk|biomaterial provider:Postlethwait lab University of Oregon|genotype:nr5a1a +/+ ; nr5a1b / |BioSampleModel:Model organism or animal                 RNA Seq of Danio rerio: 21dpf nr5a1b mutant 6 21dpf nr5a1b mutant 6 21dpf nr5a1b mutant 6 Strand specific RNA seq library prepared with BIOO NEXTflex Rapid Directional qRNA Seq kit     RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina HiSeq 4000   SRP219014     nr5a1b21dmut-6_combined_R1.fq.gz nr5a1b21dmut-6_combined_R2.fq.gz fastq fastq 3384770096.0 11207848.0 nr5a1b21dmut 6 combined R1.fq.gz 0:151 1:151 A:861183234;C:797950851;G:807513834;T:917578636;N:543541 151 151     861183234 797950851 807513834 917578636 543541 SRX6748551 SRS5298720 SRA946180 University of Oregon|Institute of Neuroscience University of Oregon 2 0.90245 0.90886 0.09636 0.07472 0.74004 0.743 0.46058 0.53685 151 151 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   United States 2019-08-21 Larval Larval Trunk Surface Structure
53707 53707 SRR10010388 SRX6748550 SRS5298719 SRP219014 PRJNA561212 RNA Seq of nr5a1a and nr5a1b mutant zebrafish PRJNA561212 Transcriptome Analysis Zebrafish were euthanized in Tricaine. We isolated gonad containing trunk of theanimals by removing the anterior of the fish from just posterior of the pectoral finand removing the caudal peduncle posterior to the anus. Trunks were individuallyhomogenized in 200ul Trizol. Total RNA was extracted using the Ribopure RNAPurification Kit ThermoFisher. Total RNA was enriched for mRNA using DynabeadsrOligodt25 ThermoFisher. We constructed indexed strand specific cDNA sequencinglibraries using the NEXTflextm qRNA seq kit BIOO Scientific. Libraryconcentrations were quantified using a Qubitr fluorometer Life Technologies normalized to a concentration of 2.3nM and multiplexed. Prior to sequencing wefurther evaluated the quality of the multiplexed library by quantitative real timePCR using the Kapa Library Quantification Kit Kapa Biosystems. Two lanes ofpaired end 150 base pair bp sequencing were performed on an Illumina HiSeq 4000.         35dpf nr5a1a   strain:AB|age:35 dpf|sex:not applicable|tissue:trunk|biomaterial provider:Postlethwait lab University of Oregon|genotype:nr5a1a / ; nr5a1b +/+|BioSampleModel:Model organism or animal                 RNA Seq of Danio rerio: 35dpf nr5a1a mutant 2 35dpf nr5a1a mutant 2 35dpf nr5a1a mutant 2 Strand specific RNA seq library prepared with BIOO NEXTflex Rapid Directional qRNA Seq kit     RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina HiSeq 4000   SRP219014     nr5a1a35dmut-2_combined_R1.fq.gz nr5a1a35dmut-2_combined_R2.fq.gz fastq fastq 1239380518.0 4103909.0 nr5a1a35dmut 2 combined R1.fq.gz 0:151 1:151 A:309089709;C:300159914;G:303434072;T:326498923;N:197900 151 151     309089709 300159914 303434072 326498923 197900 SRX6748550 SRS5298719 SRA946180 University of Oregon|Institute of Neuroscience University of Oregon 2 0.91546 0.92068 0.11935 0.07796 0.74397 0.7474 0.48962 0.61439 151 151 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   United States 2019-08-21 Juvenile Juvenile Trunk Surface Structure
53708 53708 SRR10010389 SRX6748549 SRS5298719 SRP219014 PRJNA561212 RNA Seq of nr5a1a and nr5a1b mutant zebrafish PRJNA561212 Transcriptome Analysis Zebrafish were euthanized in Tricaine. We isolated gonad containing trunk of theanimals by removing the anterior of the fish from just posterior of the pectoral finand removing the caudal peduncle posterior to the anus. Trunks were individuallyhomogenized in 200ul Trizol. Total RNA was extracted using the Ribopure RNAPurification Kit ThermoFisher. Total RNA was enriched for mRNA using DynabeadsrOligodt25 ThermoFisher. We constructed indexed strand specific cDNA sequencinglibraries using the NEXTflextm qRNA seq kit BIOO Scientific. Libraryconcentrations were quantified using a Qubitr fluorometer Life Technologies normalized to a concentration of 2.3nM and multiplexed. Prior to sequencing wefurther evaluated the quality of the multiplexed library by quantitative real timePCR using the Kapa Library Quantification Kit Kapa Biosystems. Two lanes ofpaired end 150 base pair bp sequencing were performed on an Illumina HiSeq 4000.         35dpf nr5a1a   strain:AB|age:35 dpf|sex:not applicable|tissue:trunk|biomaterial provider:Postlethwait lab University of Oregon|genotype:nr5a1a / ; nr5a1b +/+|BioSampleModel:Model organism or animal                 RNA Seq of Danio rerio: 35dpf nr5a1a mutant 1 35dpf nr5a1a mutant 1 35dpf nr5a1a mutant 1 Strand specific RNA seq library prepared with BIOO NEXTflex Rapid Directional qRNA Seq kit     RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina HiSeq 4000   SRP219014     nr5a1a35dmut-1_combined_R1.fq.gz nr5a1a35dmut-1_combined_R2.fq.gz fastq fastq 2224834906.0 7367003.0 nr5a1a35dmut 1 combined R1.fq.gz 0:151 1:151 A:541266079;C:545117817;G:559850378;T:578249036;N:351596 151 151     541266079 545117817 559850378 578249036 351596 SRX6748549 SRS5298719 SRA946180 University of Oregon|Institute of Neuroscience University of Oregon 2 0.91337 0.9214 0.12797 0.08298 0.74961 0.75483 0.45923 0.54594 151 151 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   United States 2019-08-21 Juvenile Juvenile Trunk Surface Structure
53709 53709 SRR10010390 SRX6748548 SRS5298719 SRP219014 PRJNA561212 RNA Seq of nr5a1a and nr5a1b mutant zebrafish PRJNA561212 Transcriptome Analysis Zebrafish were euthanized in Tricaine. We isolated gonad containing trunk of theanimals by removing the anterior of the fish from just posterior of the pectoral finand removing the caudal peduncle posterior to the anus. Trunks were individuallyhomogenized in 200ul Trizol. Total RNA was extracted using the Ribopure RNAPurification Kit ThermoFisher. Total RNA was enriched for mRNA using DynabeadsrOligodt25 ThermoFisher. We constructed indexed strand specific cDNA sequencinglibraries using the NEXTflextm qRNA seq kit BIOO Scientific. Libraryconcentrations were quantified using a Qubitr fluorometer Life Technologies normalized to a concentration of 2.3nM and multiplexed. Prior to sequencing wefurther evaluated the quality of the multiplexed library by quantitative real timePCR using the Kapa Library Quantification Kit Kapa Biosystems. Two lanes ofpaired end 150 base pair bp sequencing were performed on an Illumina HiSeq 4000.         35dpf nr5a1a   strain:AB|age:35 dpf|sex:not applicable|tissue:trunk|biomaterial provider:Postlethwait lab University of Oregon|genotype:nr5a1a / ; nr5a1b +/+|BioSampleModel:Model organism or animal                 RNA Seq of Danio rerio: 35dpf nr5a1a mutant 4 35dpf nr5a1a mutant 4 35dpf nr5a1a mutant 4 Strand specific RNA seq library prepared with BIOO NEXTflex Rapid Directional qRNA Seq kit     RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina HiSeq 4000   SRP219014     nr5a1a35dmut-4_combined_R1.fq.gz nr5a1a35dmut-4_combined_R2.fq.gz fastq fastq 2564347836.0 8491218.0 nr5a1a35dmut 4 combined R1.fq.gz 0:151 1:151 A:626506801;C:604109158;G:622843659;T:710461729;N:426489 151 151     626506801 604109158 622843659 710461729 426489 SRX6748548 SRS5298719 SRA946180 University of Oregon|Institute of Neuroscience University of Oregon 2 0.88743 0.91434 0.08038 0.05675 0.75562 0.75676 0.47736 0.57787 151 151 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   United States 2019-08-21 Juvenile Juvenile Trunk Surface Structure
53710 53710 SRR10010391 SRX6748547 SRS5298719 SRP219014 PRJNA561212 RNA Seq of nr5a1a and nr5a1b mutant zebrafish PRJNA561212 Transcriptome Analysis Zebrafish were euthanized in Tricaine. We isolated gonad containing trunk of theanimals by removing the anterior of the fish from just posterior of the pectoral finand removing the caudal peduncle posterior to the anus. Trunks were individuallyhomogenized in 200ul Trizol. Total RNA was extracted using the Ribopure RNAPurification Kit ThermoFisher. Total RNA was enriched for mRNA using DynabeadsrOligodt25 ThermoFisher. We constructed indexed strand specific cDNA sequencinglibraries using the NEXTflextm qRNA seq kit BIOO Scientific. Libraryconcentrations were quantified using a Qubitr fluorometer Life Technologies normalized to a concentration of 2.3nM and multiplexed. Prior to sequencing wefurther evaluated the quality of the multiplexed library by quantitative real timePCR using the Kapa Library Quantification Kit Kapa Biosystems. Two lanes ofpaired end 150 base pair bp sequencing were performed on an Illumina HiSeq 4000.         35dpf nr5a1a   strain:AB|age:35 dpf|sex:not applicable|tissue:trunk|biomaterial provider:Postlethwait lab University of Oregon|genotype:nr5a1a / ; nr5a1b +/+|BioSampleModel:Model organism or animal                 RNA Seq of Danio rerio: 35dpf nr5a1a mutant 3 35dpf nr5a1a mutant 3 35dpf nr5a1a mutant 3 Strand specific RNA seq library prepared with BIOO NEXTflex Rapid Directional qRNA Seq kit     RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina HiSeq 4000   SRP219014     nr5a1a35dmut-3_combined_R1.fq.gz nr5a1a35dmut-3_combined_R2.fq.gz fastq fastq 2109532212.0 6985206.0 nr5a1a35dmut 3 combined R1.fq.gz 0:151 1:151 A:523059463;C:502631876;G:510494846;T:572988523;N:357504 151 151     523059463 502631876 510494846 572988523 357504 SRX6748547 SRS5298719 SRA946180 University of Oregon|Institute of Neuroscience University of Oregon 2 0.89446 0.90702 0.08692 0.06146 0.75049 0.75312 0.5212 0.57721 151 151 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   United States 2019-08-21 Juvenile Juvenile Trunk Surface Structure
53711 53711 SRR10010392 SRX6748546 SRS5298718 SRP219014 PRJNA561212 RNA Seq of nr5a1a and nr5a1b mutant zebrafish PRJNA561212 Transcriptome Analysis Zebrafish were euthanized in Tricaine. We isolated gonad containing trunk of theanimals by removing the anterior of the fish from just posterior of the pectoral finand removing the caudal peduncle posterior to the anus. Trunks were individuallyhomogenized in 200ul Trizol. Total RNA was extracted using the Ribopure RNAPurification Kit ThermoFisher. Total RNA was enriched for mRNA using DynabeadsrOligodt25 ThermoFisher. We constructed indexed strand specific cDNA sequencinglibraries using the NEXTflextm qRNA seq kit BIOO Scientific. Libraryconcentrations were quantified using a Qubitr fluorometer Life Technologies normalized to a concentration of 2.3nM and multiplexed. Prior to sequencing wefurther evaluated the quality of the multiplexed library by quantitative real timePCR using the Kapa Library Quantification Kit Kapa Biosystems. Two lanes ofpaired end 150 base pair bp sequencing were performed on an Illumina HiSeq 4000.         21dpf nr5a1a   strain:AB|age:21 dpf|sex:not applicable|tissue:trunk|biomaterial provider:Postlethwait lab University of Oregon|genotype:nr5a1a / ; nr5a1b +/+|BioSampleModel:Model organism or animal                 RNA Seq of Danio rerio: 21dpf nr5a1a mutant 6 21dpf nr5a1a mutant 6 21dpf nr5a1a mutant 6 Strand specific RNA seq library prepared with BIOO NEXTflex Rapid Directional qRNA Seq kit     RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina HiSeq 4000   SRP219014     nr5a1a21dmut-6_combined_R1.fq.gz nr5a1a21dmut-6_combined_R2.fq.gz fastq fastq 2094501672.0 6935436.0 nr5a1a21dmut 6 combined R1.fq.gz 0:151 1:151 A:522046863;C:501034595;G:506909098;T:564180097;N:331019 151 151     522046863 501034595 506909098 564180097 331019 SRX6748546 SRS5298718 SRA946180 University of Oregon|Institute of Neuroscience University of Oregon 2 0.90679 0.91646 0.11189 0.07897 0.74428 0.74771 0.44274 0.53352 151 151 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   United States 2019-08-21 Larval Larval Trunk Surface Structure
53712 53712 SRR10010393 SRX6748545 SRS5298718 SRP219014 PRJNA561212 RNA Seq of nr5a1a and nr5a1b mutant zebrafish PRJNA561212 Transcriptome Analysis Zebrafish were euthanized in Tricaine. We isolated gonad containing trunk of theanimals by removing the anterior of the fish from just posterior of the pectoral finand removing the caudal peduncle posterior to the anus. Trunks were individuallyhomogenized in 200ul Trizol. Total RNA was extracted using the Ribopure RNAPurification Kit ThermoFisher. Total RNA was enriched for mRNA using DynabeadsrOligodt25 ThermoFisher. We constructed indexed strand specific cDNA sequencinglibraries using the NEXTflextm qRNA seq kit BIOO Scientific. Libraryconcentrations were quantified using a Qubitr fluorometer Life Technologies normalized to a concentration of 2.3nM and multiplexed. Prior to sequencing wefurther evaluated the quality of the multiplexed library by quantitative real timePCR using the Kapa Library Quantification Kit Kapa Biosystems. Two lanes ofpaired end 150 base pair bp sequencing were performed on an Illumina HiSeq 4000.         21dpf nr5a1a   strain:AB|age:21 dpf|sex:not applicable|tissue:trunk|biomaterial provider:Postlethwait lab University of Oregon|genotype:nr5a1a / ; nr5a1b +/+|BioSampleModel:Model organism or animal                 RNA Seq of Danio rerio: 21dpf nr5a1a mutant 5 21dpf nr5a1a mutant 5 21dpf nr5a1a mutant 5 Strand specific RNA seq library prepared with BIOO NEXTflex Rapid Directional qRNA Seq kit     RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina HiSeq 4000   SRP219014     nr5a1a21dmut-5_combined_R1.fq.gz nr5a1a21dmut-5_combined_R2.fq.gz fastq fastq 2104404856.0 6968228.0 nr5a1a21dmut 5 combined R1.fq.gz 0:151 1:151 A:536145551;C:492187666;G:501143675;T:574557684;N:370280 151 151     536145551 492187666 501143675 574557684 370280 SRX6748545 SRS5298718 SRA946180 University of Oregon|Institute of Neuroscience University of Oregon 2 0.86941 0.88309 0.08729 0.0711 0.72857 0.73048 0.47555 0.52991 151 151 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   United States 2019-08-21 Larval Larval Trunk Surface Structure
53713 53713 SRR10010394 SRX6748544 SRS5298718 SRP219014 PRJNA561212 RNA Seq of nr5a1a and nr5a1b mutant zebrafish PRJNA561212 Transcriptome Analysis Zebrafish were euthanized in Tricaine. We isolated gonad containing trunk of theanimals by removing the anterior of the fish from just posterior of the pectoral finand removing the caudal peduncle posterior to the anus. Trunks were individuallyhomogenized in 200ul Trizol. Total RNA was extracted using the Ribopure RNAPurification Kit ThermoFisher. Total RNA was enriched for mRNA using DynabeadsrOligodt25 ThermoFisher. We constructed indexed strand specific cDNA sequencinglibraries using the NEXTflextm qRNA seq kit BIOO Scientific. Libraryconcentrations were quantified using a Qubitr fluorometer Life Technologies normalized to a concentration of 2.3nM and multiplexed. Prior to sequencing wefurther evaluated the quality of the multiplexed library by quantitative real timePCR using the Kapa Library Quantification Kit Kapa Biosystems. Two lanes ofpaired end 150 base pair bp sequencing were performed on an Illumina HiSeq 4000.         21dpf nr5a1a   strain:AB|age:21 dpf|sex:not applicable|tissue:trunk|biomaterial provider:Postlethwait lab University of Oregon|genotype:nr5a1a / ; nr5a1b +/+|BioSampleModel:Model organism or animal                 RNA Seq of Danio rerio: 21dpf nr5a1a mutant 8 21dpf nr5a1a mutant 8 21dpf nr5a1a mutant 8 Strand specific RNA seq library prepared with BIOO NEXTflex Rapid Directional qRNA Seq kit     RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina HiSeq 4000   SRP219014     nr5a1a21dmut-8_combined_R1.fq.gz nr5a1a21dmut-8_combined_R2.fq.gz fastq fastq 2266701468.0 7505634.0 nr5a1a21dmut 8 combined R1.fq.gz 0:151 1:151 A:573714272;C:538051325;G:547611130;T:606957830;N:366911 151 151     573714272 538051325 547611130 606957830 366911 SRX6748544 SRS5298718 SRA946180 University of Oregon|Institute of Neuroscience University of Oregon 2 0.9024 0.90712 0.10447 0.08144 0.71687 0.72066 0.46792 0.53188 151 151 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   United States 2019-08-21 Larval Larval Trunk Surface Structure
53714 53714 SRR10010395 SRX6748543 SRS5298718 SRP219014 PRJNA561212 RNA Seq of nr5a1a and nr5a1b mutant zebrafish PRJNA561212 Transcriptome Analysis Zebrafish were euthanized in Tricaine. We isolated gonad containing trunk of theanimals by removing the anterior of the fish from just posterior of the pectoral finand removing the caudal peduncle posterior to the anus. Trunks were individuallyhomogenized in 200ul Trizol. Total RNA was extracted using the Ribopure RNAPurification Kit ThermoFisher. Total RNA was enriched for mRNA using DynabeadsrOligodt25 ThermoFisher. We constructed indexed strand specific cDNA sequencinglibraries using the NEXTflextm qRNA seq kit BIOO Scientific. Libraryconcentrations were quantified using a Qubitr fluorometer Life Technologies normalized to a concentration of 2.3nM and multiplexed. Prior to sequencing wefurther evaluated the quality of the multiplexed library by quantitative real timePCR using the Kapa Library Quantification Kit Kapa Biosystems. Two lanes ofpaired end 150 base pair bp sequencing were performed on an Illumina HiSeq 4000.         21dpf nr5a1a   strain:AB|age:21 dpf|sex:not applicable|tissue:trunk|biomaterial provider:Postlethwait lab University of Oregon|genotype:nr5a1a / ; nr5a1b +/+|BioSampleModel:Model organism or animal                 RNA Seq of Danio rerio: 21dpf nr5a1a mutant 7 21dpf nr5a1a mutant 7 21dpf nr5a1a mutant 7 Strand specific RNA seq library prepared with BIOO NEXTflex Rapid Directional qRNA Seq kit     RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina HiSeq 4000   SRP219014     nr5a1a21dmut-7_combined_R1.fq.gz nr5a1a21dmut-7_combined_R2.fq.gz fastq fastq 2754530826.0 9120963.0 nr5a1a21dmut 7 combined R1.fq.gz 0:151 1:151 A:694171422;C:632458248;G:643565340;T:783901287;N:434529 151 151     694171422 632458248 643565340 783901287 434529 SRX6748543 SRS5298718 SRA946180 University of Oregon|Institute of Neuroscience University of Oregon 2 0.88557 0.91232 0.10743 0.08366 0.73896 0.74081 0.42454 0.58309 151 151 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   United States 2019-08-21 Larval Larval Trunk Surface Structure
53715 53715 SRR10010396 SRX6748542 SRS5298717 SRP219014 PRJNA561212 RNA Seq of nr5a1a and nr5a1b mutant zebrafish PRJNA561212 Transcriptome Analysis Zebrafish were euthanized in Tricaine. We isolated gonad containing trunk of theanimals by removing the anterior of the fish from just posterior of the pectoral finand removing the caudal peduncle posterior to the anus. Trunks were individuallyhomogenized in 200ul Trizol. Total RNA was extracted using the Ribopure RNAPurification Kit ThermoFisher. Total RNA was enriched for mRNA using DynabeadsrOligodt25 ThermoFisher. We constructed indexed strand specific cDNA sequencinglibraries using the NEXTflextm qRNA seq kit BIOO Scientific. Libraryconcentrations were quantified using a Qubitr fluorometer Life Technologies normalized to a concentration of 2.3nM and multiplexed. Prior to sequencing wefurther evaluated the quality of the multiplexed library by quantitative real timePCR using the Kapa Library Quantification Kit Kapa Biosystems. Two lanes ofpaired end 150 base pair bp sequencing were performed on an Illumina HiSeq 4000.         35dpf nr5a1a nr5a1b   strain:AB|age:35 dpf|sex:not applicable|tissue:trunk|biomaterial provider:Postlethwait lab University of Oregon|genotype:nr5a1a / ; nr5a1b / |BioSampleModel:Model organism or animal                 RNA Seq of Danio rerio: 35dpf nr5a1a nr5a1b double mutant 13 35dpf nr5a1a nr5a1b double mutant 13 35dpf nr5a1a nr5a1b double mutant 13 Strand specific RNA seq library prepared with BIOO NEXTflex Rapid Directional qRNA Seq kit     RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina HiSeq 4000   SRP219014     nr5a1ab35dmut-13_combined_R1.fq.gz nr5a1ab35dmut-13_combined_R2.fq.gz fastq fastq 4623122002.0 15308351.0 nr5a1ab35dmut 13 combined R1.fq.gz 0:151 1:151 A:1157235877;C:1116162876;G:1129874932;T:1219134272;N:714045 151 151     1157235877 1116162876 1129874932 1219134272 714045 SRX6748542 SRS5298717 SRA946180 University of Oregon|Institute of Neuroscience University of Oregon 2 0.91894 0.92533 0.11425 0.08034 0.75635 0.76023 0.50951 0.61492 151 151 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   United States 2019-08-21 Juvenile Juvenile Trunk Surface Structure
53716 53716 SRR10010397 SRX6748541 SRS5298717 SRP219014 PRJNA561212 RNA Seq of nr5a1a and nr5a1b mutant zebrafish PRJNA561212 Transcriptome Analysis Zebrafish were euthanized in Tricaine. We isolated gonad containing trunk of theanimals by removing the anterior of the fish from just posterior of the pectoral finand removing the caudal peduncle posterior to the anus. Trunks were individuallyhomogenized in 200ul Trizol. Total RNA was extracted using the Ribopure RNAPurification Kit ThermoFisher. Total RNA was enriched for mRNA using DynabeadsrOligodt25 ThermoFisher. We constructed indexed strand specific cDNA sequencinglibraries using the NEXTflextm qRNA seq kit BIOO Scientific. Libraryconcentrations were quantified using a Qubitr fluorometer Life Technologies normalized to a concentration of 2.3nM and multiplexed. Prior to sequencing wefurther evaluated the quality of the multiplexed library by quantitative real timePCR using the Kapa Library Quantification Kit Kapa Biosystems. Two lanes ofpaired end 150 base pair bp sequencing were performed on an Illumina HiSeq 4000.         35dpf nr5a1a nr5a1b   strain:AB|age:35 dpf|sex:not applicable|tissue:trunk|biomaterial provider:Postlethwait lab University of Oregon|genotype:nr5a1a / ; nr5a1b / |BioSampleModel:Model organism or animal                 RNA Seq of Danio rerio: 35dpf nr5a1a nr5a1b double mutant 12 35dpf nr5a1a nr5a1b double mutant 12 35dpf nr5a1a nr5a1b double mutant 12 Strand specific RNA seq library prepared with BIOO NEXTflex Rapid Directional qRNA Seq kit     RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina HiSeq 4000   SRP219014     nr5a1ab35dmut-12_combined_R1.fq.gz nr5a1ab35dmut-12_combined_R2.fq.gz fastq fastq 2610452666.0 8643883.0 nr5a1ab35dmut 12 combined R1.fq.gz 0:151 1:151 A:648839778;C:629525229;G:642133919;T:689532605;N:421135 151 151     648839778 629525229 642133919 689532605 421135 SRX6748541 SRS5298717 SRA946180 University of Oregon|Institute of Neuroscience University of Oregon 2 0.90931 0.91675 0.11029 0.07732 0.74584 0.74937 0.48342 0.59544 151 151 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   United States 2019-08-21 Juvenile Juvenile Trunk Surface Structure
53717 53717 SRR10010398 SRX6748540 SRS5298716 SRP219014 PRJNA561212 RNA Seq of nr5a1a and nr5a1b mutant zebrafish PRJNA561212 Transcriptome Analysis Zebrafish were euthanized in Tricaine. We isolated gonad containing trunk of theanimals by removing the anterior of the fish from just posterior of the pectoral finand removing the caudal peduncle posterior to the anus. Trunks were individuallyhomogenized in 200ul Trizol. Total RNA was extracted using the Ribopure RNAPurification Kit ThermoFisher. Total RNA was enriched for mRNA using DynabeadsrOligodt25 ThermoFisher. We constructed indexed strand specific cDNA sequencinglibraries using the NEXTflextm qRNA seq kit BIOO Scientific. Libraryconcentrations were quantified using a Qubitr fluorometer Life Technologies normalized to a concentration of 2.3nM and multiplexed. Prior to sequencing wefurther evaluated the quality of the multiplexed library by quantitative real timePCR using the Kapa Library Quantification Kit Kapa Biosystems. Two lanes ofpaired end 150 base pair bp sequencing were performed on an Illumina HiSeq 4000.         35dpf nr5a1b   strain:AB|age:35 dpf|sex:not applicable|tissue:trunk|biomaterial provider:Postlethwait lab University of Oregon|genotype:nr5a1a +/+ ; nr5a1b / |BioSampleModel:Model organism or animal                 RNA Seq of Danio rerio: 35dpf nr5a1b mutant 3 35dpf nr5a1b mutant 3 35dpf nr5a1b mutant 3 Strand specific RNA seq library prepared with BIOO NEXTflex Rapid Directional qRNA Seq kit     RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina HiSeq 4000   SRP219014     nr5a1b35dmut-3_combined_R1.fq.gz nr5a1b35dmut-3_combined_R2.fq.gz fastq fastq 1943393556.0 6435078.0 nr5a1b35dmut 3 combined R1.fq.gz 0:151 1:151 A:475436905;C:468830275;G:477482918;T:521312410;N:331048 151 151     475436905 468830275 477482918 521312410 331048 SRX6748540 SRS5298716 SRA946180 University of Oregon|Institute of Neuroscience University of Oregon 2 0.90426 0.92131 0.09556 0.06323 0.75327 0.75292 0.50371 0.57748 151 151 B B biological fallback assumption illumina hiseq_era unknown poly_a unknown bulk unknown unknown   United States 2019-08-21 Juvenile Juvenile Trunk Surface Structure
67535 67535 SRR17191184 SRX13373023 SRS11277345 SRP350168 PRJNA787741 The effects of tire wear particles on zebrafish eyes PRJNA787741 Other The RNA sequencing data provides biomarkers for the phototransduction dysfunction induced by the tire wear particles       LT2 5 1 LT2 5 1   isolate:larvae|age:5 days|sex:not determined|tissue:whole body10|BioSampleModel:Model organism or animal                 RNA Seq of Danio rerio larvea LT2 5 1 LT2 5 1 The cDNA librarie was constructed from zebrafish larvae treated by low concentration tire wear particle leachate for 5 days. The libraries were sequenced using an Illumina Novaseq 6006 platform.     RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina NovaSeq 6000   SRP350168     LT2_5_1_1.fq.gz LT2_5_1_2.fq.gz fastq fastq 6355172100.0 21183907.0 LT2 5 1 1.fq.gz 0:150 1:150 A:1692532186;C:1495123900;G:1493518955;T:1673652858;N:344201 150 150     1692532186 1495123900 1493518955 1673652858 344201 SRX13373023 SRS11277345 SRA1341542 Chinese Academy of Sciences|RESEARCH CENTER FOR ECO-ENVIRONMENTAL SCIENCES Chinese Academy of Sciences 2 0.94919 0.94714 0.08463 0.08356 0.65553 0.65715 0.47802 0.48303 150 150 B B biological fallback assumption illumina novaseq_era unknown poly_a unknown bulk unknown unknown   China 2021-12-10 Larval Larval Trunk Surface Structure
67536 67536 SRR17191185 SRX13373022 SRS11277346 SRP350168 PRJNA787741 The effects of tire wear particles on zebrafish eyes PRJNA787741 Other The RNA sequencing data provides biomarkers for the phototransduction dysfunction induced by the tire wear particles       LM100 3 LM100 3   isolate:larvae|age:5 days|sex:not determined|tissue:whole body9|BioSampleModel:Model organism or animal                 RNA Seq of Danio rerio larvea LM100 3 LM100 3 The cDNA librarie was constructed from zebrafish larvae treated by high concentration road particle leachate for 5 days. The libraries were sequenced using an Illumina Novaseq 6005 platform.     RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina NovaSeq 6000   SRP350168     LM100_3_1.fq.gz LM100_3_2.fq.gz fastq fastq 6993427200.0 23311424.0 LM100 3 1.fq.gz 0:150 1:150 A:1840660966;C:1658361838;G:1666920090;T:1827424421;N:59885 150 150     1840660966 1658361838 1666920090 1827424421 59885 SRX13373022 SRS11277346 SRA1341542 Chinese Academy of Sciences|RESEARCH CENTER FOR ECO-ENVIRONMENTAL SCIENCES Chinese Academy of Sciences 2 0.95677 0.95477 0.06791 0.06756 0.65531 0.6552 0.49085 0.49017 150 150 B B biological fallback assumption illumina novaseq_era unknown poly_a unknown bulk unknown unknown   China 2021-12-10 Larval Larval Trunk Surface Structure
67537 67537 SRR17191186 SRX13373021 SRS11277344 SRP350168 PRJNA787741 The effects of tire wear particles on zebrafish eyes PRJNA787741 Other The RNA sequencing data provides biomarkers for the phototransduction dysfunction induced by the tire wear particles       LM100 2 LM100 2   isolate:larvae|age:5 days|sex:not determined|tissue:whole body8|BioSampleModel:Model organism or animal                 RNA Seq of Danio rerio larvea LM100 2 LM100 2 The cDNA librarie was constructed from zebrafish larvae treated by high concentration road particle leachate for 5 days. The libraries were sequenced using an Illumina Novaseq 6004 platform.     RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina NovaSeq 6000   SRP350168     LM100_2_1.fq.gz LM100_2_2.fq.gz fastq fastq 6428993400.0 21429978.0 LM100 2 1.fq.gz 0:150 1:150 A:1701794239;C:1523306298;G:1520155501;T:1683386473;N:350889 150 150     1701794239 1523306298 1520155501 1683386473 350889 SRX13373021 SRS11277344 SRA1341542 Chinese Academy of Sciences|RESEARCH CENTER FOR ECO-ENVIRONMENTAL SCIENCES Chinese Academy of Sciences 2 0.95457 0.95198 0.07446 0.07392 0.65484 0.65547 0.4943 0.49318 150 150 B B biological fallback assumption illumina novaseq_era unknown poly_a unknown bulk unknown unknown   China 2021-12-10 Larval Larval Trunk Surface Structure
67538 67538 SRR17191187 SRX13373020 SRS11277342 SRP350168 PRJNA787741 The effects of tire wear particles on zebrafish eyes PRJNA787741 Other The RNA sequencing data provides biomarkers for the phototransduction dysfunction induced by the tire wear particles       LM100 1 LM100 1   isolate:larvae|age:5 days|sex:not determined|tissue:whole body7|BioSampleModel:Model organism or animal                 RNA Seq of Danio rerio larvea LM100 1 LM100 1 The cDNA librarie was constructed from zebrafish larvae treated by high concentration road particle leachate for 5 days. The libraries were sequenced using an Illumina Novaseq 6003 platform.     RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina NovaSeq 6000   SRP350168     LM100_1_1.fq.gz LM100_1_2.fq.gz fastq fastq 6678576600.0 22261922.0 LM100 1 1.fq.gz 0:150 1:150 A:1773351023;C:1578046234;G:1574674518;T:1752136583;N:368242 150 150     1773351023 1578046234 1574674518 1752136583 368242 SRX13373020 SRS11277342 SRA1341542 Chinese Academy of Sciences|RESEARCH CENTER FOR ECO-ENVIRONMENTAL SCIENCES Chinese Academy of Sciences 2 0.95268 0.94949 0.08246 0.0821 0.65744 0.66011 0.49051 0.49276 150 150 B B biological fallback assumption illumina novaseq_era unknown poly_a unknown bulk unknown unknown   China 2021-12-10 Larval Larval Trunk Surface Structure
67539 67539 SRR17191188 SRX13373019 SRS11277341 SRP350168 PRJNA787741 The effects of tire wear particles on zebrafish eyes PRJNA787741 Other The RNA sequencing data provides biomarkers for the phototransduction dysfunction induced by the tire wear particles       LM10 3 LM10 3   isolate:larvae|age:5 days|sex:not determined|tissue:whole body6|BioSampleModel:Model organism or animal                 RNA Seq of Danio rerio larvea LM10 3 LM10 3 The cDNA librarie was constructed from zebrafish larvae treated by low concentration road particle leachate for 5 days. The libraries were sequenced using an Illumina Novaseq 6002 platform.     RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina NovaSeq 6000   SRP350168     LM10_3_1.fq.gz LM10_3_2.fq.gz fastq fastq 6825978000.0 22753260.0 LM10 3 1.fq.gz 0:150 1:150 A:1812535717;C:1612480097;G:1606739928;T:1793839846;N:382412 150 150     1812535717 1612480097 1606739928 1793839846 382412 SRX13373019 SRS11277341 SRA1341542 Chinese Academy of Sciences|RESEARCH CENTER FOR ECO-ENVIRONMENTAL SCIENCES Chinese Academy of Sciences 2 0.95382 0.95278 0.07718 0.07712 0.6562 0.6561 0.48018 0.48034 150 150 B B biological fallback assumption illumina novaseq_era unknown poly_a unknown bulk unknown unknown   China 2021-12-10 Larval Larval Trunk Surface Structure
67540 67540 SRR17191189 SRX13373018 SRS11277343 SRP350168 PRJNA787741 The effects of tire wear particles on zebrafish eyes PRJNA787741 Other The RNA sequencing data provides biomarkers for the phototransduction dysfunction induced by the tire wear particles       LM10 2 LM10 2   isolate:larvae|age:5 days|sex:not determined|tissue:whole body5|BioSampleModel:Model organism or animal                 RNA Seq of Danio rerio larvea LM10 2 LM10 2 The cDNA librarie was constructed from zebrafish larvae treated by low concentration road particle leachate for 5 days. The libraries were sequenced using an Illumina Novaseq 6001 platform.     RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina NovaSeq 6000   SRP350168     LM10_2_1.fq.gz LM10_2_2.fq.gz fastq fastq 6274853400.0 20916178.0 LM10 2 1.fq.gz 0:150 1:150 A:1670545321;C:1478346621;G:1473194830;T:1652427844;N:338784 150 150     1670545321 1478346621 1473194830 1652427844 338784 SRX13373018 SRS11277343 SRA1341542 Chinese Academy of Sciences|RESEARCH CENTER FOR ECO-ENVIRONMENTAL SCIENCES Chinese Academy of Sciences 2 0.95255 0.9416 0.08061 0.07916 0.65598 0.65776 0.48378 0.48631 150 150 B B biological fallback assumption illumina novaseq_era unknown poly_a unknown bulk unknown unknown   China 2021-12-10 Larval Larval Trunk Surface Structure
67541 67541 SRR17191190 SRX13373017 SRS11277338 SRP350168 PRJNA787741 The effects of tire wear particles on zebrafish eyes PRJNA787741 Other The RNA sequencing data provides biomarkers for the phototransduction dysfunction induced by the tire wear particles       LM10 1 LM10 1   isolate:larvae|age:5 days|sex:not determined|tissue:whole body4|BioSampleModel:Model organism or animal                 RNA Seq of Danio rerio larvea LM10 1 LM10 1 The cDNA librarie was constructed from zebrafish larvae treated by low concentration road particle leachate for 5 days. The libraries were sequenced using an Illumina Novaseq 6000 platform.     RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina NovaSeq 6000   SRP350168     LM10_1_1.fq.gz LM10_1_2.fq.gz fastq fastq 6634350000.0 22114500.0 LM10 1 1.fq.gz 0:150 1:150 A:1764979073;C:1564650554;G:1560184813;T:1744173580;N:361980 150 150     1764979073 1564650554 1560184813 1744173580 361980 SRX13373017 SRS11277338 SRA1341542 Chinese Academy of Sciences|RESEARCH CENTER FOR ECO-ENVIRONMENTAL SCIENCES Chinese Academy of Sciences 2 0.95247 0.94962 0.07678 0.07606 0.65648 0.65837 0.48867 0.48222 150 150 B B biological fallback assumption illumina novaseq_era unknown poly_a unknown bulk unknown unknown   China 2021-12-10 Larval Larval Trunk Surface Structure
67542 67542 SRR17191191 SRX13373016 SRS11277340 SRP350168 PRJNA787741 The effects of tire wear particles on zebrafish eyes PRJNA787741 Other The RNA sequencing data provides biomarkers for the phototransduction dysfunction induced by the tire wear particles       CK3 CK3   isolate:larvae|age:5 days|sex:not determined|tissue:whole body3|BioSampleModel:Model organism or animal                 RNA Seq of Danio rerio larvea CK3 CK3 The cDNA librarie was constructed from control group zebrafish larvae. The libraries were sequenced using an Illumina Novaseq 6002 platform.     RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina NovaSeq 6000   SRP350168     CK3_1.fq.gz CK3_2.fq.gz fastq fastq 6442858800.0 21476196.0 CK3 1.fq.gz 0:150 1:150 A:1709894381;C:1522924138;G:1523272496;T:1686418644;N:349141 150 150     1709894381 1522924138 1523272496 1686418644 349141 SRX13373016 SRS11277340 SRA1341542 Chinese Academy of Sciences|RESEARCH CENTER FOR ECO-ENVIRONMENTAL SCIENCES Chinese Academy of Sciences 2 0.9629 0.94845 0.04492 0.04355 0.73752 0.74089 0.48412 0.48472 150 150 B B biological fallback assumption illumina novaseq_era unknown poly_a unknown bulk unknown unknown   China 2021-12-10 Larval Larval Trunk Surface Structure
67543 67543 SRR17191192 SRX13373015 SRS11277339 SRP350168 PRJNA787741 The effects of tire wear particles on zebrafish eyes PRJNA787741 Other The RNA sequencing data provides biomarkers for the phototransduction dysfunction induced by the tire wear particles       LT25 3 LT25 3   isolate:larvae|age:5 days|sex:not determined|tissue:whole body15|BioSampleModel:Model organism or animal                 RNA Seq of Danio rerio larvea LT25 3 LT25 3 The cDNA librarie was constructed from zebrafish larvae treated by high concentration tire wear particle leachate for 5 days. The libraries were sequenced using an Illumina Novaseq 6011 platform.     RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina NovaSeq 6000   SRP350168     LT25_3_1.fq.gz LT25_3_2.fq.gz fastq fastq 6649696800.0 22165656.0 LT25 3 1.fq.gz 0:150 1:150 A:1773928092;C:1552739246;G:1555610815;T:1767054104;N:364543 150 150     1773928092 1552739246 1555610815 1767054104 364543 SRX13373015 SRS11277339 SRA1341542 Chinese Academy of Sciences|RESEARCH CENTER FOR ECO-ENVIRONMENTAL SCIENCES Chinese Academy of Sciences 2 0.94632 0.9444 0.04887 0.04847 0.75702 0.75763 0.45747 0.47867 150 150 B B biological fallback assumption illumina novaseq_era unknown poly_a unknown bulk unknown unknown   China 2021-12-10 Larval Larval Trunk Surface Structure
67544 67544 SRR17191193 SRX13373014 SRS11277337 SRP350168 PRJNA787741 The effects of tire wear particles on zebrafish eyes PRJNA787741 Other The RNA sequencing data provides biomarkers for the phototransduction dysfunction induced by the tire wear particles       LT25 2 LT25 2   isolate:larvae|age:5 days|sex:not determined|tissue:whole body14|BioSampleModel:Model organism or animal                 RNA Seq of Danio rerio larvea LT25 2 LT25 2 The cDNA librarie was constructed from zebrafish larvae treated by high concentration tire wear particle leachate for 5 days. The libraries were sequenced using an Illumina Novaseq 6010 platform.     RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina NovaSeq 6000   SRP350168     LT25_2_1.fq.gz LT25_2_2.fq.gz fastq fastq 7081101000.0 23603670.0 LT25 2 1.fq.gz 0:150 1:150 A:1854482058;C:1695572359;G:1695167836;T:1835494035;N:384712 150 150     1854482058 1695572359 1695167836 1835494035 384712 SRX13373014 SRS11277337 SRA1341542 Chinese Academy of Sciences|RESEARCH CENTER FOR ECO-ENVIRONMENTAL SCIENCES Chinese Academy of Sciences 2 0.95537 0.95349 0.07622 0.0755 0.6566 0.6574 0.49112 0.4885 150 150 B B biological fallback assumption illumina novaseq_era unknown poly_a unknown bulk unknown unknown   China 2021-12-10 Larval Larval Trunk Surface Structure
67545 67545 SRR17191194 SRX13373013 SRS11277333 SRP350168 PRJNA787741 The effects of tire wear particles on zebrafish eyes PRJNA787741 Other The RNA sequencing data provides biomarkers for the phototransduction dysfunction induced by the tire wear particles       LT25 1 LT25 1   isolate:larvae|age:5 days|sex:not determined|tissue:whole body13|BioSampleModel:Model organism or animal                 RNA Seq of Danio rerio larvea LT25 1 LT25 1 The cDNA librarie was constructed from zebrafish larvae treated by high concentration tire wear particle leachate for 5 days. The libraries were sequenced using an Illumina Novaseq 6009 platform.     RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina NovaSeq 6000   SRP350168     LT25_1_1.fq.gz LT25_1_2.fq.gz fastq fastq 6667605600.0 22225352.0 LT25 1 1.fq.gz 0:150 1:150 A:1740379719;C:1600230796;G:1601983926;T:1724655312;N:355847 150 150     1740379719 1600230796 1601983926 1724655312 355847 SRX13373013 SRS11277333 SRA1341542 Chinese Academy of Sciences|RESEARCH CENTER FOR ECO-ENVIRONMENTAL SCIENCES Chinese Academy of Sciences 2 0.95447 0.9529 0.0792 0.07864 0.65498 0.65502 0.48602 0.48538 150 150 B B biological fallback assumption illumina novaseq_era unknown poly_a unknown bulk unknown unknown   China 2021-12-10 Larval Larval Trunk Surface Structure
67546 67546 SRR17191195 SRX13373012 SRS11277336 SRP350168 PRJNA787741 The effects of tire wear particles on zebrafish eyes PRJNA787741 Other The RNA sequencing data provides biomarkers for the phototransduction dysfunction induced by the tire wear particles       LT2 5 3 LT2 5 3   isolate:larvae|age:5 days|sex:not determined|tissue:whole body12|BioSampleModel:Model organism or animal                 RNA Seq of Danio rerio larvea LT2 5 3 LT2 5 3 The cDNA librarie was constructed from zebrafish larvae treated by low concentration tire wear particle leachate for 5 days. The libraries were sequenced using an Illumina Novaseq 6008 platform.     RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina NovaSeq 6000   SRP350168     LT2_5_3_1.fq.gz LT2_5_3_2.fq.gz fastq fastq 6828759900.0 22762533.0 LT2 5 3 1.fq.gz 0:150 1:150 A:1812242219;C:1614183257;G:1611383597;T:1790226183;N:724644 150 150     1812242219 1614183257 1611383597 1790226183 724644 SRX13373012 SRS11277336 SRA1341542 Chinese Academy of Sciences|RESEARCH CENTER FOR ECO-ENVIRONMENTAL SCIENCES Chinese Academy of Sciences 2 0.95078 0.94825 0.08641 0.08506 0.66306 0.66316 0.49287 0.49262 150 150 B B biological fallback assumption illumina novaseq_era unknown poly_a unknown bulk unknown unknown   China 2021-12-10 Larval Larval Trunk Surface Structure
67547 67547 SRR17191196 SRX13373011 SRS11277334 SRP350168 PRJNA787741 The effects of tire wear particles on zebrafish eyes PRJNA787741 Other The RNA sequencing data provides biomarkers for the phototransduction dysfunction induced by the tire wear particles       LT2 5 2 LT2 5 2   isolate:larvae|age:5 days|sex:not determined|tissue:whole body11|BioSampleModel:Model organism or animal                 RNA Seq of Danio rerio larvea LT2 5 2 LT2 5 2 The cDNA librarie was constructed from zebrafish larvae treated by low concentration tire wear particle leachate for 5 days. The libraries were sequenced using an Illumina Novaseq 6007 platform.     RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina NovaSeq 6000   SRP350168     LT2_5_2_1.fq.gz LT2_5_2_2.fq.gz fastq fastq 6310300800.0 21034336.0 LT2 5 2 1.fq.gz 0:150 1:150 A:1677760007;C:1486958406;G:1485763386;T:1659474382;N:344619 150 150     1677760007 1486958406 1485763386 1659474382 344619 SRX13373011 SRS11277334 SRA1341542 Chinese Academy of Sciences|RESEARCH CENTER FOR ECO-ENVIRONMENTAL SCIENCES Chinese Academy of Sciences 2 0.95038 0.94901 0.08122 0.08028 0.65616 0.65829 0.4867 0.48612 150 150 B B biological fallback assumption illumina novaseq_era unknown poly_a unknown bulk unknown unknown   China 2021-12-10 Larval Larval Trunk Surface Structure
67548 67548 SRR17191197 SRX13373010 SRS11277335 SRP350168 PRJNA787741 The effects of tire wear particles on zebrafish eyes PRJNA787741 Other The RNA sequencing data provides biomarkers for the phototransduction dysfunction induced by the tire wear particles       CK2 CK2   isolate:larvae|age:5 days|sex:not determined|tissue:whole body2|BioSampleModel:Model organism or animal                 RNA Seq of Danio rerio larvea CK2 CK2 The cDNA librarie was constructed from control group zebrafish larvae. The libraries were sequenced using an Illumina Novaseq 6001 platform.     RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina NovaSeq 6000   SRP350168     CK2_1.fq.gz CK2_2.fq.gz fastq fastq 6603630900.0 22012103.0 CK2 1.fq.gz 0:150 1:150 A:1753952423;C:1560198614;G:1556107876;T:1733008704;N:363283 150 150     1753952423 1560198614 1556107876 1733008704 363283 SRX13373010 SRS11277335 SRA1341542 Chinese Academy of Sciences|RESEARCH CENTER FOR ECO-ENVIRONMENTAL SCIENCES Chinese Academy of Sciences 2 0.94909 0.94729 0.06551 0.06446 0.6621 0.66393 0.48571 0.48779 150 150 B B biological fallback assumption illumina novaseq_era unknown poly_a unknown bulk unknown unknown   China 2021-12-10 Larval Larval Trunk Surface Structure
67549 67549 SRR17191198 SRX13373009 SRS11277332 SRP350168 PRJNA787741 The effects of tire wear particles on zebrafish eyes PRJNA787741 Other The RNA sequencing data provides biomarkers for the phototransduction dysfunction induced by the tire wear particles       CK1 CK1   isolate:larvae|age:5 days|sex:not determined|tissue:whole body1|BioSampleModel:Model organism or animal                 RNA Seq of Danio rerio larvea CK1 CK1 The cDNA librarie was constructed from control group zebrafish larvae. The libraries were sequenced using an Illumina Novaseq 6000 platform.     RNA-Seq TRANSCRIPTOMIC PolyA PAIRED ILLUMINA Illumina NovaSeq 6000   SRP350168     CK1_1.fq.gz CK1_2.fq.gz fastq fastq 7408269000.0 24694230.0 CK1 1.fq.gz 0:150 1:150 A:1967467604;C:1749366598;G:1746566984;T:1944369697;N:498117 150 150     1967467604 1749366598 1746566984 1944369697 498117 SRX13373009 SRS11277332 SRA1341542 Chinese Academy of Sciences|RESEARCH CENTER FOR ECO-ENVIRONMENTAL SCIENCES Chinese Academy of Sciences 2 0.95273 0.95109 0.07165 0.07191 0.65947 0.6616 0.48253 0.47225 150 150 B B biological fallback assumption illumina novaseq_era unknown poly_a unknown bulk unknown unknown   China 2021-12-10 Larval Larval Trunk Surface Structure

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CREATE TABLE run_metadata("run.accession" VARCHAR, "experiment.accession" VARCHAR, "sample.accession" VARCHAR, "study.accession" VARCHAR, bioproject VARCHAR, "study.title" VARCHAR, "study.alias" VARCHAR, "study.type" VARCHAR, "study.abstract" VARCHAR, "study.attributes" VARCHAR, "study.PMIDs" VARCHAR, "sample.description" VARCHAR, "sample.title" VARCHAR, "sample.alias" VARCHAR, "sample.centername" VARCHAR, "sample.attributes" VARCHAR, "GEOsample.title" VARCHAR, "GEOsample.dataprocessing" VARCHAR, "GEOsample.source" VARCHAR, "GEOsample.treatmentprotocol" VARCHAR, "GEOsample.extractprotocol" VARCHAR, "GEOsample.growthprotocol" VARCHAR, "GEOsample.characteristics" VARCHAR, "GEOsample.accession" VARCHAR, "experiment.title" VARCHAR, "experiment.alias" VARCHAR, "experiment.library_name" VARCHAR, "experiment.design_description" VARCHAR, "experiment.library_construction_protocol" VARCHAR, "experiment.attributes" VARCHAR, "experiment.library_strategy" VARCHAR, "experiment.library_source" VARCHAR, "experiment.library_selection" VARCHAR, "experiment.library_layout" VARCHAR, "experiment.platform" VARCHAR, "experiment.instrument_model" VARCHAR, "experiment.spot_descriptor" VARCHAR, "experiment.study_ref" VARCHAR, "run.title" VARCHAR, "run.attributes" VARCHAR, "run.filename" VARCHAR, "run.semantic_name" VARCHAR, "run.total_bases" DOUBLE, "run.total_spots" DOUBLE, "run.alias" VARCHAR, "run.read_lengths" VARCHAR, "run.base_counts" VARCHAR, "run.r1_length" BIGINT, "run.r2_length" BIGINT, "run.r3_length" BIGINT, "run.r4_length" BIGINT, "run.Acount" BIGINT, "run.Ccount" BIGINT, "run.Gcount" BIGINT, "run.Tcount" BIGINT, "run.Ncount" BIGINT, "run.experiment" VARCHAR, "run.pool_member" VARCHAR, "submission.accession" VARCHAR, "submission.srasource" VARCHAR, "submission.bioprojectsource" VARCHAR, "seqdetective.n_mates" BIGINT, "seqdetective.mapping_rate.mate1" DOUBLE, "seqdetective.mapping_rate.mate2" DOUBLE, "seqdetective.nofeature_rate.mate1" DOUBLE, "seqdetective.nofeature_rate.mate2" DOUBLE, "seqdetective.sparsity.mate1" DOUBLE, "seqdetective.sparsity.mate2" DOUBLE, "seqdetective.pos_strand_rate.mate1" DOUBLE, "seqdetective.pos_strand_rate.mate2" DOUBLE, "seqdetective.readlen.mate1" BIGINT, "seqdetective.readlen.mate2" BIGINT, "seqdetective.judgement.mate1" VARCHAR, "seqdetective.judgement.mate2" VARCHAR, "seqdetective.judgement.reason" VARCHAR, platform_family VARCHAR, instrument_generation VARCHAR, read_bias VARCHAR, selection_class VARCHAR, prep_kit VARCHAR, sc_or_bulk VARCHAR, tech_class VARCHAR, technology VARCHAR, tech_variant VARCHAR, "submission.bioprojectsource.country" VARCHAR, earliest_date DATE, devstage_curation VARCHAR, devstage_curation_coarse VARCHAR, tissue_curation VARCHAR, tissue_curation_coarse VARCHAR);;
CREATE INDEX idx_run_bioproject ON run_metadata(bioproject);;
CREATE INDEX idx_run_run_accession ON run_metadata("run.accession");;