run_metadata
25 rows where experiment.library_layout = "PAIRED" and experiment.library_selection = "CAGE"
This data as json, CSV (advanced)
| Link | rowid ▼ | run.accession | experiment.accession | sample.accession | study.accession | bioproject | study.title | study.alias | study.type | study.abstract | study.attributes | study.PMIDs | sample.description | sample.title | sample.alias | sample.centername | sample.attributes | GEOsample.title | GEOsample.dataprocessing | GEOsample.source | GEOsample.treatmentprotocol | GEOsample.extractprotocol | GEOsample.growthprotocol | GEOsample.characteristics | GEOsample.accession | experiment.title | experiment.alias | experiment.library_name | experiment.design_description | experiment.library_construction_protocol | experiment.attributes | experiment.library_strategy | experiment.library_source | experiment.library_selection | experiment.library_layout | experiment.platform | experiment.instrument_model | experiment.spot_descriptor | experiment.study_ref | run.title | run.attributes | run.filename | run.semantic_name | run.total_bases | run.total_spots | run.alias | run.read_lengths | run.base_counts | run.r1_length | run.r2_length | run.r3_length | run.r4_length | run.Acount | run.Ccount | run.Gcount | run.Tcount | run.Ncount | run.experiment | run.pool_member | submission.accession | submission.srasource | submission.bioprojectsource | seqdetective.n_mates | seqdetective.mapping_rate.mate1 | seqdetective.mapping_rate.mate2 | seqdetective.nofeature_rate.mate1 | seqdetective.nofeature_rate.mate2 | seqdetective.sparsity.mate1 | seqdetective.sparsity.mate2 | seqdetective.pos_strand_rate.mate1 | seqdetective.pos_strand_rate.mate2 | seqdetective.readlen.mate1 | seqdetective.readlen.mate2 | seqdetective.judgement.mate1 | seqdetective.judgement.mate2 | seqdetective.judgement.reason | platform_family | instrument_generation | read_bias | selection_class | prep_kit | sc_or_bulk | tech_class | technology | tech_variant | submission.bioprojectsource.country | earliest_date | devstage_curation | devstage_curation_coarse | tissue_curation | tissue_curation_coarse |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 37185 | 37185 | SRR1010334 | SRX363385 | SRS490049 | SRP030770 | PRJNA222234 | Danio rerio Targeted Locus Loci | PRJNA222234 | Transcriptome Analysis | Goal of this study is to functionally validate regulatory elements within sf3a2 gene promoter which guide transcription start site selection for that promoter in the zebrafish oocyte. | pubmed:24531765 | stable transgenic line with mutated sf3a2 promoter construct driving expression of mCherry | Generic sample from Danio rerio | D. rerio MUTL7F transgenic line | strain:AB strain MUTL7F line|development stage:high 3.3 hpf | D. rerio MUTL7F transgenic line single locus CAGE mutated sf3a2:mCherry transgene | D. rerio MUTL7F transgenic line single locus CAGE mutated sf3a2:mCherry transgene | 1 | 1 | OTHER | TRANSCRIPTOMIC | CAGE | PAIRED | ILLUMINA | Illumina HiSeq 2000 | <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>69</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>35</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> | SRP030770 | MUTL7F_transgene_R1.fastq MUTL7F_transgene_R2.fastq | fastq fastq | 30678849.0 | 444621.0 | D. rerio MUTL7F transgenic line single locus CAGE mutated sf3a2:mCherry transgene | 0:34 1:35 | A:5994534;C:7452275;G:9596502;T:7623459;N:12079 | 34 | 35 | 5994534 | 7452275 | 9596502 | 7623459 | 12079 | SRX363385 | SRS490049 | SRA104816 | University of Bergen | ZEPROME consortium | 2 | 0.38487 | 0.37445 | 0.0013 | 9e-05 | 0.99841 | 0.99991 | 0.00309 | 0.00012 | 34 | 35 | B | B | biological fallback assumption | illumina | hiseq_era | unknown | cage | unknown | bulk | unknown | unknown | Unknown | 2014-02-10 | Blastula | Embryo | Undetermined | Embryo Imprecise | |||||||||||||||||
| 37186 | 37186 | SRR1010333 | SRX363384 | SRS490049 | SRP030770 | PRJNA222234 | Danio rerio Targeted Locus Loci | PRJNA222234 | Transcriptome Analysis | Goal of this study is to functionally validate regulatory elements within sf3a2 gene promoter which guide transcription start site selection for that promoter in the zebrafish oocyte. | pubmed:24531765 | stable transgenic line with mutated sf3a2 promoter construct driving expression of mCherry | Generic sample from Danio rerio | D. rerio MUTL7F transgenic line | strain:AB strain MUTL7F line|development stage:high 3.3 hpf | D. rerio MUTL7F transgenic line single locus CAGE endogeneous sf3a2 | D. rerio MUTL7F transgenic line single locus CAGE endogeneous sf3a2 | 1 | 1 | OTHER | TRANSCRIPTOMIC | CAGE | PAIRED | ILLUMINA | Illumina HiSeq 2000 | <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>69</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>35</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> | SRP030770 | MUTL7F_endogenous_R2.fastq MUTL7F_endogenous_R1.fastq | fastq fastq | 78367233.0 | 1135757.0 | D. rerio MUTL7F transgenic line single locus CAGE endogeneous sf3a2 | 0:34 1:35 | A:21010895;C:20506800;G:21883789;T:14935556;N:30193 | 34 | 35 | 21010895 | 20506800 | 21883789 | 14935556 | 30193 | SRX363384 | SRS490049 | SRA104816 | University of Bergen | ZEPROME consortium | 2 | 0.56643 | 0.02972 | 6e-05 | 0.00056 | 0.99965 | 0.99995 | 0.00017 | 0.05769 | 34 | 35 | B | T | mate2 technical by mapping diff | illumina | hiseq_era | unknown | cage | unknown | bulk | unknown | unknown | Unknown | 2014-02-10 | Blastula | Embryo | Undetermined | Embryo Imprecise | |||||||||||||||||
| 37187 | 37187 | SRR1010332 | SRX363383 | SRS490048 | SRP030770 | PRJNA222234 | Danio rerio Targeted Locus Loci | PRJNA222234 | Transcriptome Analysis | Goal of this study is to functionally validate regulatory elements within sf3a2 gene promoter which guide transcription start site selection for that promoter in the zebrafish oocyte. | pubmed:24531765 | stable transgenic line with mutated sf3a2 promoter construct driving expression of mCherry | Generic sample from Danio rerio | D. rerio MUTL6F transgenic line | strain:AB strain MUTL6F line|development stage:high 3.3 hpf | D. rerio MUTL6F transgenic line single locus CAGE mutated sf3a2:mCherry transgene | D. rerio MUTL6F transgenic line single locus CAGE mutated sf3a2:mCherry transgene | 1 | 1 | OTHER | TRANSCRIPTOMIC | CAGE | PAIRED | ILLUMINA | Illumina HiSeq 2000 | <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>69</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>35</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> | SRP030770 | MUTL6F_transgene_R2.fastq MUTL6F_transgene_R1.fastq | fastq fastq | 36838272.0 | 533888.0 | D. rerio MUTL6F transgenic line single locus CAGE mutated sf3a2:mCherry transgene | 0:34 1:35 | A:7267891;C:8939911;G:11513757;T:9101861;N:14852 | 34 | 35 | 7267891 | 8939911 | 11513757 | 9101861 | 14852 | SRX363383 | SRS490048 | SRA104816 | University of Bergen | ZEPROME consortium | 2 | 0.35237 | 0.20738 | 0.00196 | 4e-05 | 0.9991 | 0.99987 | 0.00131 | 0.00022 | 34 | 35 | B | B | mate2-mate1 similar by mapping diff | illumina | hiseq_era | unknown | cage | unknown | bulk | unknown | unknown | Unknown | 2014-02-10 | Blastula | Embryo | Undetermined | Embryo Imprecise | |||||||||||||||||
| 37188 | 37188 | SRR1010331 | SRX363382 | SRS490048 | SRP030770 | PRJNA222234 | Danio rerio Targeted Locus Loci | PRJNA222234 | Transcriptome Analysis | Goal of this study is to functionally validate regulatory elements within sf3a2 gene promoter which guide transcription start site selection for that promoter in the zebrafish oocyte. | pubmed:24531765 | stable transgenic line with mutated sf3a2 promoter construct driving expression of mCherry | Generic sample from Danio rerio | D. rerio MUTL6F transgenic line | strain:AB strain MUTL6F line|development stage:high 3.3 hpf | D. rerio MUTL6F transgenic line single locus CAGE endogeneous sf3a2 | D. rerio MUTL6F transgenic line single locus CAGE endogeneous sf3a2 | 1 | 1 | OTHER | TRANSCRIPTOMIC | CAGE | PAIRED | ILLUMINA | Illumina HiSeq 2000 | <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>69</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>35</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> | SRP030770 | MUTL6F_endogenous_R1.fastq MUTL6F_endogenous_R2.fastq | fastq fastq | 67887582.0 | 983878.0 | D. rerio MUTL6F transgenic line single locus CAGE endogeneous sf3a2 | 0:34 1:35 | A:18224546;C:17687428;G:19119778;T:12829091;N:26739 | 34 | 35 | 18224546 | 17687428 | 19119778 | 12829091 | 26739 | SRX363382 | SRS490048 | SRA104816 | University of Bergen | ZEPROME consortium | 2 | 0.57761 | 0.02201 | 0.0001 | 0.00048 | 0.99975 | 0.99991 | 0.00011 | 0.02247 | 34 | 35 | B | T | mate2 technical by mapping diff | illumina | hiseq_era | unknown | cage | unknown | bulk | unknown | unknown | Unknown | 2014-02-10 | Blastula | Embryo | Undetermined | Embryo Imprecise | |||||||||||||||||
| 37189 | 37189 | SRR1010330 | SRX363381 | SRS490047 | SRP030770 | PRJNA222234 | Danio rerio Targeted Locus Loci | PRJNA222234 | Transcriptome Analysis | Goal of this study is to functionally validate regulatory elements within sf3a2 gene promoter which guide transcription start site selection for that promoter in the zebrafish oocyte. | pubmed:24531765 | stable transgenic line with mutated sf3a2 promoter construct driving expression of mCherry | Generic sample from Danio rerio | D. rerio MUTL5F transgenic line | strain:AB strain MUTL5F line|development stage:high 3.3 hpf | D. rerio MUTL5F transgenic line single locus CAGE mutated sf3a2:mCherry transgene | D. rerio MUTL5F transgenic line single locus CAGE mutated sf3a2:mCherry transgene | 1 | 1 | OTHER | TRANSCRIPTOMIC | CAGE | PAIRED | ILLUMINA | Illumina HiSeq 2000 | <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>69</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>35</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> | SRP030770 | MUTL5F_transgene_R1.fastq MUTL5F_transgene_R2.fastq | fastq fastq | 48750708.0 | 706532.0 | D. rerio MUTL5F transgenic line single locus CAGE mutated sf3a2:mCherry transgene | 0:34 1:35 | A:9468865;C:11866504;G:15193605;T:12203213;N:18521 | 34 | 35 | 9468865 | 11866504 | 15193605 | 12203213 | 18521 | SRX363381 | SRS490047 | SRA104816 | University of Bergen | ZEPROME consortium | 2 | 0.37677 | 0.39423 | 0.0018 | 0.00013 | 0.99801 | 0.99989 | 0.00295 | 0.00011 | 34 | 35 | B | B | biological fallback assumption | illumina | hiseq_era | unknown | cage | unknown | bulk | unknown | unknown | Unknown | 2014-02-10 | Blastula | Embryo | Undetermined | Embryo Imprecise | |||||||||||||||||
| 37190 | 37190 | SRR1010329 | SRX363380 | SRS490047 | SRP030770 | PRJNA222234 | Danio rerio Targeted Locus Loci | PRJNA222234 | Transcriptome Analysis | Goal of this study is to functionally validate regulatory elements within sf3a2 gene promoter which guide transcription start site selection for that promoter in the zebrafish oocyte. | pubmed:24531765 | stable transgenic line with mutated sf3a2 promoter construct driving expression of mCherry | Generic sample from Danio rerio | D. rerio MUTL5F transgenic line | strain:AB strain MUTL5F line|development stage:high 3.3 hpf | D. rerio MUTL5F transgenic line single locus CAGE endogeneous sf3a2 | D. rerio MUTL5F transgenic line single locus CAGE endogeneous sf3a2 | 1 | 1 | OTHER | TRANSCRIPTOMIC | CAGE | PAIRED | ILLUMINA | Illumina HiSeq 2000 | <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>69</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>35</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> | SRP030770 | MUTL5F_endogenous_R1.fastq MUTL5F_endogenous_R2.fastq | fastq fastq | 85325883.0 | 1236607.0 | D. rerio MUTL5F transgenic line single locus CAGE endogeneous sf3a2 | 0:34 1:35 | A:22831000;C:22316529;G:23804395;T:16340044;N:33915 | 34 | 35 | 22831000 | 22316529 | 23804395 | 16340044 | 33915 | SRX363380 | SRS490047 | SRA104816 | University of Bergen | ZEPROME consortium | 2 | 0.59785 | 0.01902 | 0.00632 | 0.00081 | 0.99941 | 0.99993 | 0.00056 | 0.11235 | 34 | 35 | B | T | mate2 technical by mapping diff | illumina | hiseq_era | unknown | cage | unknown | bulk | unknown | unknown | Unknown | 2014-02-10 | Blastula | Embryo | Undetermined | Embryo Imprecise | |||||||||||||||||
| 37191 | 37191 | SRR1010328 | SRX363379 | SRS490046 | SRP030770 | PRJNA222234 | Danio rerio Targeted Locus Loci | PRJNA222234 | Transcriptome Analysis | Goal of this study is to functionally validate regulatory elements within sf3a2 gene promoter which guide transcription start site selection for that promoter in the zebrafish oocyte. | pubmed:24531765 | stable transgenic line with mutated sf3a2 promoter construct driving expression of mCherry | Generic sample from Danio rerio | D. rerio MUTL2F transgenic line | strain:AB strain MUTL2F line|development stage:high 3.3 hpf | D. rerio MUTL2F transgenic line single locus CAGE mutated sf3a2:mCherry transgene | D. rerio MUTL2F transgenic line single locus CAGE mutated sf3a2:mCherry transgene | 1 | 1 | OTHER | TRANSCRIPTOMIC | CAGE | PAIRED | ILLUMINA | Illumina HiSeq 2000 | <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>69</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>35</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> | SRP030770 | MUTL2F_transgene_R1.fastq MUTL2F_transgene_R2.fastq | fastq fastq | 68247900.0 | 989100.0 | D. rerio MUTL2F transgenic line single locus CAGE mutated sf3a2:mCherry transgene | 0:34 1:35 | A:13037092;C:16853304;G:21557175;T:16772703;N:27626 | 34 | 35 | 13037092 | 16853304 | 21557175 | 16772703 | 27626 | SRX363379 | SRS490046 | SRA104816 | University of Bergen | ZEPROME consortium | 2 | 0.37746 | 0.14836 | 0.00135 | 0.00215 | 0.99912 | 0.99995 | 0.00128 | 0.0 | 34 | 35 | B | B | mate2-mate1 similar by mapping diff | illumina | hiseq_era | unknown | cage | unknown | bulk | unknown | unknown | Unknown | 2014-02-10 | Blastula | Embryo | Undetermined | Embryo Imprecise | |||||||||||||||||
| 37192 | 37192 | SRR1010327 | SRX363378 | SRS490046 | SRP030770 | PRJNA222234 | Danio rerio Targeted Locus Loci | PRJNA222234 | Transcriptome Analysis | Goal of this study is to functionally validate regulatory elements within sf3a2 gene promoter which guide transcription start site selection for that promoter in the zebrafish oocyte. | pubmed:24531765 | stable transgenic line with mutated sf3a2 promoter construct driving expression of mCherry | Generic sample from Danio rerio | D. rerio MUTL2F transgenic line | strain:AB strain MUTL2F line|development stage:high 3.3 hpf | D. rerio MUTL2F transgenic line single locus CAGE endogeneous sf3a2 | D. rerio MUTL2F transgenic line single locus CAGE endogeneous sf3a2 | 1 | 1 | OTHER | TRANSCRIPTOMIC | CAGE | PAIRED | ILLUMINA | Illumina HiSeq 2000 | <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>69</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>35</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> | SRP030770 | MUTL2F_endogenous_R1.fastq MUTL2F_endogenous_R2.fastq | fastq fastq | 85300215.0 | 1236235.0 | D. rerio MUTL2F transgenic line single locus CAGE endogeneous sf3a2 | 0:34 1:35 | A:22860161;C:22364533;G:24027064;T:16015109;N:33348 | 34 | 35 | 22860161 | 22364533 | 24027064 | 16015109 | 33348 | SRX363378 | SRS490046 | SRA104816 | University of Bergen | ZEPROME consortium | 2 | 0.57324 | 0.0117 | 0.01138 | 0.00049 | 0.99943 | 0.99993 | 0.00087 | 0.02941 | 34 | 35 | B | T | mate2 technical by mapping diff | illumina | hiseq_era | unknown | cage | unknown | bulk | unknown | unknown | Unknown | 2014-02-10 | Blastula | Embryo | Undetermined | Embryo Imprecise | |||||||||||||||||
| 37193 | 37193 | SRR1010326 | SRX363377 | SRS490045 | SRP030770 | PRJNA222234 | Danio rerio Targeted Locus Loci | PRJNA222234 | Transcriptome Analysis | Goal of this study is to functionally validate regulatory elements within sf3a2 gene promoter which guide transcription start site selection for that promoter in the zebrafish oocyte. | pubmed:24531765 | stable transgenic line with wild type sf3a2 promoter construct driving expression of mCherry | Generic sample from Danio rerio | D. rerio WTL3F transgenic line | strain:AB strain WTL3F line|development stage:high 3.3 hpf | D. rerio WTL3F transgenic line single locus CAGE wild type sf3a2:mCherry transgene | D. rerio WTL3F transgenic line single locus CAGE wild type sf3a2:mCherry transgene | 1 | 1 | OTHER | TRANSCRIPTOMIC | CAGE | PAIRED | ILLUMINA | Illumina HiSeq 2000 | <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>69</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>35</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> | SRP030770 | WTL3F_transgene_R1.fastq WTL3F_transgene_R2.fastq | fastq fastq | 36051879.0 | 522491.0 | D. rerio WTL3F transgenic line single locus CAGE wild type sf3a2:mCherry transgene | 0:34 1:35 | A:6990071;C:8680451;G:10883119;T:9484027;N:14211 | 34 | 35 | 6990071 | 8680451 | 10883119 | 9484027 | 14211 | SRX363377 | SRS490045 | SRA104816 | University of Bergen | ZEPROME consortium | 2 | 0.43354 | 0.14606 | 0.00247 | 9e-05 | 0.99896 | 0.99991 | 0.00217 | 0.00032 | 34 | 35 | B | B | mate2-mate1 similar by mapping diff | illumina | hiseq_era | unknown | cage | unknown | bulk | unknown | unknown | Unknown | 2014-02-10 | Blastula | Embryo | Undetermined | Embryo Imprecise | |||||||||||||||||
| 37194 | 37194 | SRR1010325 | SRX363376 | SRS490045 | SRP030770 | PRJNA222234 | Danio rerio Targeted Locus Loci | PRJNA222234 | Transcriptome Analysis | Goal of this study is to functionally validate regulatory elements within sf3a2 gene promoter which guide transcription start site selection for that promoter in the zebrafish oocyte. | pubmed:24531765 | stable transgenic line with wild type sf3a2 promoter construct driving expression of mCherry | Generic sample from Danio rerio | D. rerio WTL3F transgenic line | strain:AB strain WTL3F line|development stage:high 3.3 hpf | D. rerio WTL3F transgenic line single locus CAGE endogenous sf3a2 | D. rerio WTL3F transgenic line single locus CAGE endogenous sf3a2 | 1 | 1 | OTHER | TRANSCRIPTOMIC | CAGE | PAIRED | ILLUMINA | Illumina HiSeq 2000 | <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>69</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>35</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> | SRP030770 | WTL3F_endogenous_R1.fastq WTL3F_endogenous_R2.fastq | fastq fastq | 78469284.0 | 1137236.0 | D. rerio WTL3F transgenic line single locus CAGE endogenous sf3a2 | 0:34 1:35 | A:21059562;C:20597989;G:21976657;T:14804744;N:30332 | 34 | 35 | 21059562 | 20597989 | 21976657 | 14804744 | 30332 | SRX363376 | SRS490045 | SRA104816 | University of Bergen | ZEPROME consortium | 2 | 0.54772 | 0.02239 | 3e-05 | 0.0 | 0.99979 | 0.99993 | 0.00012 | 0.06756 | 34 | 35 | B | T | mate2 technical by mapping diff | illumina | hiseq_era | unknown | cage | unknown | bulk | unknown | unknown | Unknown | 2014-02-10 | Blastula | Embryo | Undetermined | Embryo Imprecise | |||||||||||||||||
| 37195 | 37195 | SRR1010324 | SRX363375 | SRS490044 | SRP030770 | PRJNA222234 | Danio rerio Targeted Locus Loci | PRJNA222234 | Transcriptome Analysis | Goal of this study is to functionally validate regulatory elements within sf3a2 gene promoter which guide transcription start site selection for that promoter in the zebrafish oocyte. | pubmed:24531765 | stable transgenic line with wild type sf3a2 promoter construct driving expression of mCherry | Generic sample from Danio rerio | D. rerio WTL2F transgenic line | strain:AB strain WTL2F line|development stage:high 3.3 hpf | D. rerio WTL2F transgenic line single locus CAGE wild type sf3a2:mCherry transgene | D. rerio WTL2F transgenic line single locus CAGE wild type sf3a2:mCherry transgene | 1 | 1 | OTHER | TRANSCRIPTOMIC | CAGE | PAIRED | ILLUMINA | Illumina HiSeq 2000 | <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>69</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>35</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> | SRP030770 | WTL2F_transgene_R1.fastq WTL2F_transgene_R2.fastq | fastq fastq | 67052544.0 | 971776.0 | D. rerio WTL2F transgenic line single locus CAGE wild type sf3a2:mCherry transgene | 0:34 1:35 | A:12413417;C:16491074;G:20506887;T:17614500;N:26666 | 34 | 35 | 12413417 | 16491074 | 20506887 | 17614500 | 26666 | SRX363375 | SRS490044 | SRA104816 | University of Bergen | ZEPROME consortium | 2 | 0.47234 | 0.16966 | 0.00033 | 0.00039 | 0.99918 | 0.99993 | 0.00051 | 0.0 | 34 | 35 | B | B | mate2-mate1 similar by mapping diff | illumina | hiseq_era | unknown | cage | unknown | bulk | unknown | unknown | Unknown | 2014-02-10 | Blastula | Embryo | Undetermined | Embryo Imprecise | |||||||||||||||||
| 37196 | 37196 | SRR1010323 | SRX363374 | SRS490044 | SRP030770 | PRJNA222234 | Danio rerio Targeted Locus Loci | PRJNA222234 | Transcriptome Analysis | Goal of this study is to functionally validate regulatory elements within sf3a2 gene promoter which guide transcription start site selection for that promoter in the zebrafish oocyte. | pubmed:24531765 | stable transgenic line with wild type sf3a2 promoter construct driving expression of mCherry | Generic sample from Danio rerio | D. rerio WTL2F transgenic line | strain:AB strain WTL2F line|development stage:high 3.3 hpf | D. rerio WTL2F transgenic line single locus CAGE endogenous sf3a2 | D. rerio WTL2F transgenic line single locus CAGE endogenous sf3a2 | 1 | 1 | OTHER | TRANSCRIPTOMIC | CAGE | PAIRED | ILLUMINA | Illumina HiSeq 2000 | <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>69</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>35</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> | SRP030770 | WTL2F_endogenous_R1.fastq WTL2F_endogenous_R2.fastq | fastq fastq | 71493591.0 | 1036139.0 | D. rerio WTL2F transgenic line single locus CAGE endogenous sf3a2 | 0:34 1:35 | A:19123161;C:18705556;G:20092193;T:13545180;N:27501 | 34 | 35 | 19123161 | 18705556 | 20092193 | 13545180 | 27501 | SRX363374 | SRS490044 | SRA104816 | University of Bergen | ZEPROME consortium | 2 | 0.56581 | 0.02161 | 0.00279 | 0.00056 | 0.99949 | 0.99991 | 0.00025 | 0.09333 | 34 | 35 | B | T | mate2 technical by mapping diff | illumina | hiseq_era | unknown | cage | unknown | bulk | unknown | unknown | Unknown | 2014-02-10 | Blastula | Embryo | Undetermined | Embryo Imprecise | |||||||||||||||||
| 37197 | 37197 | SRR1010322 | SRX363361 | SRS490033 | SRP030770 | PRJNA222234 | Danio rerio Targeted Locus Loci | PRJNA222234 | Transcriptome Analysis | Goal of this study is to functionally validate regulatory elements within sf3a2 gene promoter which guide transcription start site selection for that promoter in the zebrafish oocyte. | pubmed:24531765 | stable transgenic line with wild type sf3a2 promoter construct driving expression of mCherry | Generic sample from Danio rerio | D. rerio WTL1F transgenic line | strain:AB strain WTL1F line|dev stage:high 3.3 hpf | D. rerio WTL1F transgenic line single locus CAGE wild type sf3a2:mCherry transgene | D. rerio WTL1F transgenic line single locus CAGE wild type sf3a2:mCherry transgene | 1 | 1 | OTHER | TRANSCRIPTOMIC | CAGE | PAIRED | ILLUMINA | Illumina HiSeq 2000 | <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>69</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>35</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> | SRP030770 | WTL1F_transgene_R1.fastq WTL1F_transgene_R2.fastq | fastq fastq | 95222070.0 | 1380030.0 | D. rerio WTL1F transgenic line single locus CAGE wild type sf3a2:mCherry transgene | 0:34 1:35 | A:17371572;C:23537350;G:29495884;T:24779827;N:37437 | 34 | 35 | 17371572 | 23537350 | 29495884 | 24779827 | 37437 | SRX363361 | SRS490033 | SRA104816 | University of Bergen | ZEPROME consortium | 2 | 0.44573 | 0.30978 | 0.00036 | 0.00019 | 0.99943 | 0.99991 | 0.00023 | 0.00124 | 34 | 35 | B | B | mate2-mate1 similar by mapping diff | illumina | hiseq_era | unknown | cage | unknown | bulk | unknown | unknown | Unknown | 2014-02-10 | Blastula | Embryo | Undetermined | Embryo Imprecise | |||||||||||||||||
| 37198 | 37198 | SRR1010321 | SRX363360 | SRS490033 | SRP030770 | PRJNA222234 | Danio rerio Targeted Locus Loci | PRJNA222234 | Transcriptome Analysis | Goal of this study is to functionally validate regulatory elements within sf3a2 gene promoter which guide transcription start site selection for that promoter in the zebrafish oocyte. | pubmed:24531765 | stable transgenic line with wild type sf3a2 promoter construct driving expression of mCherry | Generic sample from Danio rerio | D. rerio WTL1F transgenic line | strain:AB strain WTL1F line|dev stage:high 3.3 hpf | D. rerio WTL1F transgenic line single locus CAGE endogenous sf3a2 | D. rerio WTL1F transgenic line single locus CAGE endogeneous sf3a2 | 1 | 1 | OTHER | TRANSCRIPTOMIC | CAGE | PAIRED | ILLUMINA | Illumina HiSeq 2000 | <SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>69</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>35</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR> | SRP030770 | WTL1F_endogenous_R1.fastq WTL1F_endogenous_R2.fastq | fastq fastq | 64955082.0 | 941378.0 | D. rerio WTL1F transgenic line single locus CAGE endogeneous sf3a2 | 0:34 1:35 | A:17404375;C:16997013;G:18318738;T:12210960;N:23996 | 34 | 35 | 17404375 | 16997013 | 18318738 | 12210960 | 23996 | SRX363360 | SRS490033 | SRA104816 | University of Bergen | ZEPROME consortium | 2 | 0.55528 | 0.02138 | 9e-05 | 0.00135 | 0.99963 | 0.99991 | 9e-05 | 0.10169 | 34 | 35 | B | T | mate2 technical by mapping diff | illumina | hiseq_era | unknown | cage | unknown | bulk | unknown | unknown | Unknown | 2014-02-10 | Blastula | Embryo | Undetermined | Embryo Imprecise | |||||||||||||||||
| 55270 | 55270 | SRR10215486 | SRX6935169 | SRS5465204 | SRP223930 | PRJNA575342 | CAGE /CappedRNA sequencig | PRJNA575342 | Other | CAGE and full length capped RNA sequencing for identification of transcription start TSS utilisation during Zebrafish Danio rerio embryonic development | S06 Prim5 | strain:AB|dev stage:Prim 5|sex:N/A|tissue:whole embryo|BioSampleModel:Model organism or animal | CAGE seq of Danio rerio whole embryo Prim 5 stage | Prim5 nAnTiCAGE | Prim5 nAnTiCAGE | nAnTiCAGE | RNA-Seq | TRANSCRIPTOMIC | CAGE | PAIRED | ILLUMINA | Illumina HiSeq 2500 | SRP223930 | S06_Prim5_nAnTiCAGE_1.fastq.gz S06_Prim5_nAnTiCAGE_2.fastq.gz | fastq fastq | 2672456099.0 | 13565767.0 | S06 Prim5 nAnTiCAGE 1.fastq.gz | 0:97 1:100 | A:641958912;C:678208106;G:686299870;T:662301091;N:3688120 | 97 | 100 | 641958912 | 678208106 | 686299870 | 662301091 | 3688120 | SRX6935169 | SRS5465204 | SRA971223 | University of Birmingham|Cancer and Genomic Sciences | University of Birmingham | 2 | 0.94637 | 0.95975 | 0.15027 | 0.16177 | 0.74119 | 0.7441 | 0.50314 | 0.54386 | 97 | 100 | B | B | biological fallback assumption | illumina | hiseq_era | full_length | cage | unknown | bulk | unknown | unknown | United Kingdom | 2019-10-02 | Pharyngula | Embryo | Whole Organism | All anatomical structures | |||||||||||||||||||||
| 55271 | 55271 | SRR10215487 | SRX6935168 | SRS5465203 | SRP223930 | PRJNA575342 | CAGE /CappedRNA sequencig | PRJNA575342 | Other | CAGE and full length capped RNA sequencing for identification of transcription start TSS utilisation during Zebrafish Danio rerio embryonic development | S05 4 Somies | strain:AB|dev stage:4 5 somites|sex:N/A|tissue:whole embryo|BioSampleModel:Model organism or animal | CAGE seq of Danio rerio whole embryo 4 5 somites stage | 4Somites nAnTiCAGE | 4Somites nAnTiCAGE | nAnTiCAGE | RNA-Seq | TRANSCRIPTOMIC | CAGE | PAIRED | ILLUMINA | Illumina HiSeq 2500 | SRP223930 | S05_4Somites_nAnTiCAGE_1.fastq.gz S05_4Somites_nAnTiCAGE_2.fastq.gz | fastq fastq | 1532850499.0 | 7780967.0 | S05 4Somites nAnTiCAGE 1.fastq.gz | 0:97 1:100 | A:368505591;C:387404999;G:393120996;T:381726796;N:2092117 | 97 | 100 | 368505591 | 387404999 | 393120996 | 381726796 | 2092117 | SRX6935168 | SRS5465203 | SRA971223 | University of Birmingham|Cancer and Genomic Sciences | University of Birmingham | 2 | 0.93818 | 0.96364 | 0.10425 | 0.1218 | 0.75116 | 0.74852 | 0.54456 | 0.54636 | 97 | 100 | B | B | biological fallback assumption | illumina | hiseq_era | full_length | cage | unknown | bulk | unknown | unknown | United Kingdom | 2019-10-02 | Segmentation | Embryo | Whole Organism | All anatomical structures | |||||||||||||||||||||
| 55275 | 55275 | SRR10215491 | SRX6935164 | SRS5465199 | SRP223930 | PRJNA575342 | CAGE /CappedRNA sequencig | PRJNA575342 | Other | CAGE and full length capped RNA sequencing for identification of transcription start TSS utilisation during Zebrafish Danio rerio embryonic development | S01 1Cell | strain:AB|dev stage:1cell|sex:N/A|tissue:whole embryo|BioSampleModel:Model organism or animal | CAGE seq of Danio rerio whole embryo 1 cell stage | 1Cell nAnTiCAGE | 1Cell nAnTiCAGE | nAnTiCAGE | RNA-Seq | TRANSCRIPTOMIC | CAGE | PAIRED | ILLUMINA | Illumina HiSeq 2500 | SRP223930 | S01_1Cells_nAnTiCAGE_1.fastq.gz S01_1Cells_nAnTiCAGE_2.fastq.gz | fastq fastq | 1193891117.0 | 6060361.0 | S01 1Cells nAnTiCAGE 1.fastq.gz | 0:97 1:100 | A:286037352;C:298140071;G:310668851;T:297430066;N:1614777 | 97 | 100 | 286037352 | 298140071 | 310668851 | 297430066 | 1614777 | SRX6935164 | SRS5465199 | SRA971223 | University of Birmingham|Cancer and Genomic Sciences | University of Birmingham | 2 | 0.93733 | 0.97059 | 0.08219 | 0.07881 | 0.76682 | 0.75834 | 0.53879 | 0.54537 | 97 | 100 | B | B | biological fallback assumption | illumina | hiseq_era | full_length | cage | unknown | bulk | unknown | unknown | United Kingdom | 2019-10-02 | Zygote | Embryo | Whole Organism | All anatomical structures | |||||||||||||||||||||
| 68254 | 68254 | SRR18327318 | SRX14464504 | SRS11744581 | SRP356302 | PRJNA799647 | Epigenomic analysis reveals prevalent contribution of transposable elements to cis regulatory elements tissue specific expression and alternative promoters in zebrafish | PRJNA799647 | Other | Transposable elements TEs encode regulatory elements that impact gene expression in multiple species yet a comprehensive analysis of zebrafish TEs in the context of gene regulation is lacking. Here we systematically investigate the epigenomic and transcriptomic landscape of TEs across eleven adult zebrafish tissues using multidimensional sequencing data. We find that TEs contribute substantially to a diverse array of regulatory elements in the zebrafish genome and that 37% of TEs are positioned in active regulatory states in adult zebrafish tissues. We identify TE subfamilies enriched in highly specific regulatory elements among different tissues. We use transcript assembly to discover TE derived transcriptional units expressed in a tissue specific manner. Finally we show that novel TE derived promoters can initiate tissue specific transcription of alternative isoforms. This work provides a 10 comprehensive profile of TE activity across normal zebrafish tissues shedding light on mechanisms underlying the regulation of gene expression in this widely used model organism. | zebrafish TU testis rep2 | strain:Tuebingen|isolate:rep2|age:6 mpf|dev stage:adult|sex:male|tissue:testis|BioSampleModel:Model organism or animal | nanoCAGE seq of Danio rerio Tubingen: adult male testis shallow | nanoCAGE zebrafish TU testis rep2 | nanoCAGE zebrafish TU testis rep2 | nanoCAGE seq with polyA pulldown | RNA-Seq | TRANSCRIPTOMIC | CAGE | PAIRED | ILLUMINA | NextSeq 500 | SRP356302 | nano_TUzfish_combined_deep_testis_BR2.R1.fastq.gz nano_TUzfish_combined_deep_testis_BR2.R2.fastq.gz | fastq fastq | 2408261400.0 | 15886644.0 | nano TUzfish combined deep testis BR2.R1.fastq.gz | 0:75.80 1:75.80 | A:589293804;C:562741604;G:604867589;T:650872884;N:485519 | 75 | 75 | 589293804 | 562741604 | 604867589 | 650872884 | 485519 | SRX14464504 | SRS11744581 | SRA1385043 | Washington University in St. Louis|Department of Genetics | Washington University in St. Louis | 2 | 0.05991 | 0.93925 | 0.00631 | 0.04834 | 0.91364 | 0.67671 | 0.54402 | 0.49323 | 150 | 150 | T | B | mate1 technical by mapping diff | illumina | nextseq | unknown | cage | unknown | bulk | unknown | unknown | United States | 2022-03-17 | Adult | Adult | Gonad | Reproductive System | |||||||||||||||||||||
| 68255 | 68255 | SRR18327319 | SRX14464503 | SRS11744582 | SRP356302 | PRJNA799647 | Epigenomic analysis reveals prevalent contribution of transposable elements to cis regulatory elements tissue specific expression and alternative promoters in zebrafish | PRJNA799647 | Other | Transposable elements TEs encode regulatory elements that impact gene expression in multiple species yet a comprehensive analysis of zebrafish TEs in the context of gene regulation is lacking. Here we systematically investigate the epigenomic and transcriptomic landscape of TEs across eleven adult zebrafish tissues using multidimensional sequencing data. We find that TEs contribute substantially to a diverse array of regulatory elements in the zebrafish genome and that 37% of TEs are positioned in active regulatory states in adult zebrafish tissues. We identify TE subfamilies enriched in highly specific regulatory elements among different tissues. We use transcript assembly to discover TE derived transcriptional units expressed in a tissue specific manner. Finally we show that novel TE derived promoters can initiate tissue specific transcription of alternative isoforms. This work provides a 10 comprehensive profile of TE activity across normal zebrafish tissues shedding light on mechanisms underlying the regulation of gene expression in this widely used model organism. | zebrafish TU testis rep1 | strain:Tuebingen|isolate:rep1|age:6 mpf|dev stage:adult|sex:male|tissue:testis|BioSampleModel:Model organism or animal | nanoCAGE seq of Danio rerio Tubingen: adult male testis shallow | nanoCAGE zebrafish TU testis rep1 | nanoCAGE zebrafish TU testis rep1 | nanoCAGE seq with polyA pulldown | RNA-Seq | TRANSCRIPTOMIC | CAGE | PAIRED | ILLUMINA | NextSeq 500 | SRP356302 | nano_TUzfish_combined_deep_testis_BR1.R1.fastq.gz nano_TUzfish_combined_deep_testis_BR1.R2.fastq.gz | fastq fastq | 2655464100.0 | 17518322.0 | nano TUzfish combined deep testis BR1.R1.fastq.gz | 0:75.79 1:75.79 | A:648423774;C:624425183;G:667510545;T:714557704;N:546894 | 75 | 75 | 648423774 | 624425183 | 667510545 | 714557704 | 546894 | SRX14464503 | SRS11744582 | SRA1385043 | Washington University in St. Louis|Department of Genetics | Washington University in St. Louis | 2 | 0.05205 | 0.9337 | 0.00433 | 0.0458 | 0.92086 | 0.68631 | 0.5426 | 0.50321 | 150 | 150 | T | B | mate1 technical by mapping diff | illumina | nextseq | unknown | cage | unknown | bulk | unknown | unknown | United States | 2022-03-17 | Adult | Adult | Gonad | Reproductive System | |||||||||||||||||||||
| 68256 | 68256 | SRR18327320 | SRX14464502 | SRS11744580 | SRP356302 | PRJNA799647 | Epigenomic analysis reveals prevalent contribution of transposable elements to cis regulatory elements tissue specific expression and alternative promoters in zebrafish | PRJNA799647 | Other | Transposable elements TEs encode regulatory elements that impact gene expression in multiple species yet a comprehensive analysis of zebrafish TEs in the context of gene regulation is lacking. Here we systematically investigate the epigenomic and transcriptomic landscape of TEs across eleven adult zebrafish tissues using multidimensional sequencing data. We find that TEs contribute substantially to a diverse array of regulatory elements in the zebrafish genome and that 37% of TEs are positioned in active regulatory states in adult zebrafish tissues. We identify TE subfamilies enriched in highly specific regulatory elements among different tissues. We use transcript assembly to discover TE derived transcriptional units expressed in a tissue specific manner. Finally we show that novel TE derived promoters can initiate tissue specific transcription of alternative isoforms. This work provides a 10 comprehensive profile of TE activity across normal zebrafish tissues shedding light on mechanisms underlying the regulation of gene expression in this widely used model organism. | zebrafish TU brain rep2 | strain:Tuebingen|isolate:rep2|age:6 mpf|dev stage:adult|sex:female|tissue:brain|BioSampleModel:Model organism or animal | nanoCAGE seq of Danio rerio Tubingen: adult male brain shallow | nanoCAGE zebrafish TU brain rep2 | nanoCAGE zebrafish TU brain rep2 | nanoCAGE seq with polyA pulldown | RNA-Seq | TRANSCRIPTOMIC | CAGE | PAIRED | ILLUMINA | NextSeq 500 | SRP356302 | nano_TUzfish_combined_deep_brain_BR2.R1.fastq.gz nano_TUzfish_combined_deep_brain_BR2.R2.fastq.gz | fastq fastq | 2120070300.0 | 13983927.0 | nano TUzfish combined deep brain BR2.R1.fastq.gz | 0:75.80 1:75.80 | A:521597394;C:502052046;G:539140115;T:556851976;N:428769 | 75 | 75 | 521597394 | 502052046 | 539140115 | 556851976 | 428769 | SRX14464502 | SRS11744580 | SRA1385043 | Washington University in St. Louis|Department of Genetics | Washington University in St. Louis | 2 | 0.05946 | 0.93361 | 0.00648 | 0.07284 | 0.91739 | 0.70025 | 0.57506 | 0.48669 | 150 | 150 | T | B | mate1 technical by mapping diff | illumina | nextseq | unknown | cage | unknown | bulk | unknown | unknown | United States | 2022-03-17 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||||
| 68257 | 68257 | SRR18327321 | SRX14464501 | SRS11744579 | SRP356302 | PRJNA799647 | Epigenomic analysis reveals prevalent contribution of transposable elements to cis regulatory elements tissue specific expression and alternative promoters in zebrafish | PRJNA799647 | Other | Transposable elements TEs encode regulatory elements that impact gene expression in multiple species yet a comprehensive analysis of zebrafish TEs in the context of gene regulation is lacking. Here we systematically investigate the epigenomic and transcriptomic landscape of TEs across eleven adult zebrafish tissues using multidimensional sequencing data. We find that TEs contribute substantially to a diverse array of regulatory elements in the zebrafish genome and that 37% of TEs are positioned in active regulatory states in adult zebrafish tissues. We identify TE subfamilies enriched in highly specific regulatory elements among different tissues. We use transcript assembly to discover TE derived transcriptional units expressed in a tissue specific manner. Finally we show that novel TE derived promoters can initiate tissue specific transcription of alternative isoforms. This work provides a 10 comprehensive profile of TE activity across normal zebrafish tissues shedding light on mechanisms underlying the regulation of gene expression in this widely used model organism. | zebrafish TU brain rep1 | strain:Tuebingen|isolate:rep1|age:6 mpf|dev stage:adult|sex:female|tissue:brain|BioSampleModel:Model organism or animal | nanoCAGE seq of Danio rerio Tubingen: adult male brain shallow | nanoCAGE zebrafish TU brain rep1 | nanoCAGE zebrafish TU brain rep1 | nanoCAGE seq with polyA pulldown | RNA-Seq | TRANSCRIPTOMIC | CAGE | PAIRED | ILLUMINA | NextSeq 500 | SRP356302 | nano_TUzfish_combined_deep_brain_BR1.R1.fastq.gz nano_TUzfish_combined_deep_brain_BR1.R2.fastq.gz | fastq fastq | 1958802000.0 | 12919047.0 | nano TUzfish combined deep brain BR1.R1.fastq.gz | 0:75.81 1:75.81 | A:485271176;C:461368873;G:496521163;T:515247447;N:393341 | 75 | 75 | 485271176 | 461368873 | 496521163 | 515247447 | 393341 | SRX14464501 | SRS11744579 | SRA1385043 | Washington University in St. Louis|Department of Genetics | Washington University in St. Louis | 2 | 0.0666 | 0.93487 | 0.00689 | 0.0747 | 0.91175 | 0.70027 | 0.57062 | 0.49364 | 150 | 150 | T | B | mate1 technical by mapping diff | illumina | nextseq | unknown | cage | unknown | bulk | unknown | unknown | United States | 2022-03-17 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||||
| 68258 | 68258 | SRR17709722 | SRX13872770 | SRS11744581 | SRP356302 | PRJNA799647 | Epigenomic analysis reveals prevalent contribution of transposable elements to cis regulatory elements tissue specific expression and alternative promoters in zebrafish | PRJNA799647 | Other | Transposable elements TEs encode regulatory elements that impact gene expression in multiple species yet a comprehensive analysis of zebrafish TEs in the context of gene regulation is lacking. Here we systematically investigate the epigenomic and transcriptomic landscape of TEs across eleven adult zebrafish tissues using multidimensional sequencing data. We find that TEs contribute substantially to a diverse array of regulatory elements in the zebrafish genome and that 37% of TEs are positioned in active regulatory states in adult zebrafish tissues. We identify TE subfamilies enriched in highly specific regulatory elements among different tissues. We use transcript assembly to discover TE derived transcriptional units expressed in a tissue specific manner. Finally we show that novel TE derived promoters can initiate tissue specific transcription of alternative isoforms. This work provides a 10 comprehensive profile of TE activity across normal zebrafish tissues shedding light on mechanisms underlying the regulation of gene expression in this widely used model organism. | zebrafish TU testis rep2 | strain:Tuebingen|isolate:rep2|age:6 mpf|dev stage:adult|sex:male|tissue:testis|BioSampleModel:Model organism or animal | nanoCAGE seq of Danio rerio Tubingen: adult male testis | nanoCAGE zebrafish TU testis rep2 | nanoCAGE zebrafish TU testis shallow rep2 | nanoCAGE seq with polyA pulldown | RNA-Seq | TRANSCRIPTOMIC | CAGE | PAIRED | ILLUMINA | NextSeq 500 | SRP356302 | nanoCAGE_zebrafish_TU_testis_rep2.R1.fastq.gz nanoCAGE_zebrafish_TU_testis_rep2.R2.fastq.gz | fastq fastq | 1257997200.0 | 8218216.0 | nanoCAGE zebrafish TU testis rep2.R1.fastq.gz | 0:76.54 1:76.54 | A:307675653;C:294445668;G:315944501;T:339570175;N:361203 | 76 | 76 | 307675653 | 294445668 | 315944501 | 339570175 | 361203 | SRX13872770 | SRS11744581 | SRA1360415 | Washington University in St. Louis|Department of Genetics | Washington University in St. Louis | 2 | 0.06846 | 0.93772 | 0.00675 | 0.04699 | 0.90293 | 0.67986 | 0.52867 | 0.49221 | 150 | 150 | T | B | mate1 technical by mapping diff | illumina | nextseq | unknown | cage | unknown | bulk | unknown | unknown | United States | 2022-01-23 | Adult | Adult | Gonad | Reproductive System | |||||||||||||||||||||
| 68259 | 68259 | SRR17709723 | SRX13872769 | SRS11744582 | SRP356302 | PRJNA799647 | Epigenomic analysis reveals prevalent contribution of transposable elements to cis regulatory elements tissue specific expression and alternative promoters in zebrafish | PRJNA799647 | Other | Transposable elements TEs encode regulatory elements that impact gene expression in multiple species yet a comprehensive analysis of zebrafish TEs in the context of gene regulation is lacking. Here we systematically investigate the epigenomic and transcriptomic landscape of TEs across eleven adult zebrafish tissues using multidimensional sequencing data. We find that TEs contribute substantially to a diverse array of regulatory elements in the zebrafish genome and that 37% of TEs are positioned in active regulatory states in adult zebrafish tissues. We identify TE subfamilies enriched in highly specific regulatory elements among different tissues. We use transcript assembly to discover TE derived transcriptional units expressed in a tissue specific manner. Finally we show that novel TE derived promoters can initiate tissue specific transcription of alternative isoforms. This work provides a 10 comprehensive profile of TE activity across normal zebrafish tissues shedding light on mechanisms underlying the regulation of gene expression in this widely used model organism. | zebrafish TU testis rep1 | strain:Tuebingen|isolate:rep1|age:6 mpf|dev stage:adult|sex:male|tissue:testis|BioSampleModel:Model organism or animal | nanoCAGE seq of Danio rerio Tubingen: adult male testis | nanoCAGE zebrafish TU testis rep1 | nanoCAGE zebrafish TU testis shallow rep1 | nanoCAGE seq with polyA pulldown | RNA-Seq | TRANSCRIPTOMIC | CAGE | PAIRED | ILLUMINA | NextSeq 500 | SRP356302 | nanoCAGE_zebrafish_TU_testis_rep1.R1.fastq.gz nanoCAGE_zebrafish_TU_testis_rep1.R2.fastq.gz | fastq fastq | 1351312800.0 | 8823980.0 | nanoCAGE zebrafish TU testis rep1.R1.fastq.gz | 0:76.57 1:76.57 | A:329750139;C:318355694;G:339653380;T:363149300;N:404287 | 76 | 76 | 329750139 | 318355694 | 339653380 | 363149300 | 404287 | SRX13872769 | SRS11744582 | SRA1360415 | Washington University in St. Louis|Department of Genetics | Washington University in St. Louis | 2 | 0.06051 | 0.93247 | 0.00473 | 0.0437 | 0.91088 | 0.68943 | 0.52768 | 0.49868 | 150 | 150 | T | B | mate1 technical by mapping diff | illumina | nextseq | unknown | cage | unknown | bulk | unknown | unknown | United States | 2022-01-23 | Adult | Adult | Gonad | Reproductive System | |||||||||||||||||||||
| 68260 | 68260 | SRR17709724 | SRX13872768 | SRS11744580 | SRP356302 | PRJNA799647 | Epigenomic analysis reveals prevalent contribution of transposable elements to cis regulatory elements tissue specific expression and alternative promoters in zebrafish | PRJNA799647 | Other | Transposable elements TEs encode regulatory elements that impact gene expression in multiple species yet a comprehensive analysis of zebrafish TEs in the context of gene regulation is lacking. Here we systematically investigate the epigenomic and transcriptomic landscape of TEs across eleven adult zebrafish tissues using multidimensional sequencing data. We find that TEs contribute substantially to a diverse array of regulatory elements in the zebrafish genome and that 37% of TEs are positioned in active regulatory states in adult zebrafish tissues. We identify TE subfamilies enriched in highly specific regulatory elements among different tissues. We use transcript assembly to discover TE derived transcriptional units expressed in a tissue specific manner. Finally we show that novel TE derived promoters can initiate tissue specific transcription of alternative isoforms. This work provides a 10 comprehensive profile of TE activity across normal zebrafish tissues shedding light on mechanisms underlying the regulation of gene expression in this widely used model organism. | zebrafish TU brain rep2 | strain:Tuebingen|isolate:rep2|age:6 mpf|dev stage:adult|sex:female|tissue:brain|BioSampleModel:Model organism or animal | nanoCAGE seq of Danio rerio Tubingen: adult female brain | nanoCAGE zebrafish TU brain rep2 | nanoCAGE zebrafish TU brain shallow rep2 | nanoCAGE seq with polyA pulldown | RNA-Seq | TRANSCRIPTOMIC | CAGE | PAIRED | ILLUMINA | NextSeq 500 | SRP356302 | nanoCAGE_zebrafish_TU_brain_rep2.R1.fastq.gz nanoCAGE_zebrafish_TU_brain_rep2.R2.fastq.gz | fastq fastq | 1114647450.0 | 7281108.0 | nanoCAGE zebrafish TU brain rep2.R1.fastq.gz | 0:76.54 1:76.54 | A:273967636;C:264512491;G:283567362;T:292276557;N:323404 | 76 | 76 | 273967636 | 264512491 | 283567362 | 292276557 | 323404 | SRX13872768 | SRS11744580 | SRA1360415 | Washington University in St. Louis|Department of Genetics | Washington University in St. Louis | 2 | 0.06736 | 0.93327 | 0.00663 | 0.0712 | 0.90792 | 0.70283 | 0.55337 | 0.4859 | 150 | 150 | T | B | mate1 technical by mapping diff | illumina | nextseq | unknown | cage | unknown | bulk | unknown | unknown | United States | 2022-01-23 | Adult | Adult | Brain | Nervous System | |||||||||||||||||||||
| 68261 | 68261 | SRR17709725 | SRX13872767 | SRS11744579 | SRP356302 | PRJNA799647 | Epigenomic analysis reveals prevalent contribution of transposable elements to cis regulatory elements tissue specific expression and alternative promoters in zebrafish | PRJNA799647 | Other | Transposable elements TEs encode regulatory elements that impact gene expression in multiple species yet a comprehensive analysis of zebrafish TEs in the context of gene regulation is lacking. Here we systematically investigate the epigenomic and transcriptomic landscape of TEs across eleven adult zebrafish tissues using multidimensional sequencing data. We find that TEs contribute substantially to a diverse array of regulatory elements in the zebrafish genome and that 37% of TEs are positioned in active regulatory states in adult zebrafish tissues. We identify TE subfamilies enriched in highly specific regulatory elements among different tissues. We use transcript assembly to discover TE derived transcriptional units expressed in a tissue specific manner. Finally we show that novel TE derived promoters can initiate tissue specific transcription of alternative isoforms. This work provides a 10 comprehensive profile of TE activity across normal zebrafish tissues shedding light on mechanisms underlying the regulation of gene expression in this widely used model organism. | zebrafish TU brain rep1 | strain:Tuebingen|isolate:rep1|age:6 mpf|dev stage:adult|sex:female|tissue:brain|BioSampleModel:Model organism or animal | nanoCAGE seq of Danio rerio Tubingen: adult female brain | nanoCAGE zebrafish TU brain rep1 | nanoCAGE zebrafish TU brain shallow rep1 | nanoCAGE seq with polyA pulldown | RNA-Seq | TRANSCRIPTOMIC | CAGE | PAIRED | ILLUMINA | NextSeq 500 | SRP356302 | nanoCAGE_zebrafish_TU_brain_rep1.R1.fastq.gz nanoCAGE_zebrafish_TU_brain_rep1.R2.fastq.gz | fastq fastq | 1040194800.0 | 6794999.0 | nanoCAGE zebrafish TU brain rep1.R1.fastq.gz | 0:76.54 1:76.54 | A:257427323;C:245563114;G:263853643;T:273054571;N:296149 | 76 | 76 | 257427323 | 245563114 | 263853643 | 273054571 | 296149 | SRX13872767 | SRS11744579 | SRA1360415 | Washington University in St. Louis|Department of Genetics | Washington University in St. Louis | 2 | 0.07308 | 0.93461 | 0.00714 | 0.07167 | 0.9051 | 0.70199 | 0.56162 | 0.48821 | 150 | 150 | T | B | mate1 technical by mapping diff | illumina | nextseq | unknown | cage | unknown | bulk | unknown | unknown | United States | 2022-01-23 | Adult | Adult | Brain | Nervous System |
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CREATE TABLE run_metadata("run.accession" VARCHAR, "experiment.accession" VARCHAR, "sample.accession" VARCHAR, "study.accession" VARCHAR, bioproject VARCHAR, "study.title" VARCHAR, "study.alias" VARCHAR, "study.type" VARCHAR, "study.abstract" VARCHAR, "study.attributes" VARCHAR, "study.PMIDs" VARCHAR, "sample.description" VARCHAR, "sample.title" VARCHAR, "sample.alias" VARCHAR, "sample.centername" VARCHAR, "sample.attributes" VARCHAR, "GEOsample.title" VARCHAR, "GEOsample.dataprocessing" VARCHAR, "GEOsample.source" VARCHAR, "GEOsample.treatmentprotocol" VARCHAR, "GEOsample.extractprotocol" VARCHAR, "GEOsample.growthprotocol" VARCHAR, "GEOsample.characteristics" VARCHAR, "GEOsample.accession" VARCHAR, "experiment.title" VARCHAR, "experiment.alias" VARCHAR, "experiment.library_name" VARCHAR, "experiment.design_description" VARCHAR, "experiment.library_construction_protocol" VARCHAR, "experiment.attributes" VARCHAR, "experiment.library_strategy" VARCHAR, "experiment.library_source" VARCHAR, "experiment.library_selection" VARCHAR, "experiment.library_layout" VARCHAR, "experiment.platform" VARCHAR, "experiment.instrument_model" VARCHAR, "experiment.spot_descriptor" VARCHAR, "experiment.study_ref" VARCHAR, "run.title" VARCHAR, "run.attributes" VARCHAR, "run.filename" VARCHAR, "run.semantic_name" VARCHAR, "run.total_bases" DOUBLE, "run.total_spots" DOUBLE, "run.alias" VARCHAR, "run.read_lengths" VARCHAR, "run.base_counts" VARCHAR, "run.r1_length" BIGINT, "run.r2_length" BIGINT, "run.r3_length" BIGINT, "run.r4_length" BIGINT, "run.Acount" BIGINT, "run.Ccount" BIGINT, "run.Gcount" BIGINT, "run.Tcount" BIGINT, "run.Ncount" BIGINT, "run.experiment" VARCHAR, "run.pool_member" VARCHAR, "submission.accession" VARCHAR, "submission.srasource" VARCHAR, "submission.bioprojectsource" VARCHAR, "seqdetective.n_mates" BIGINT, "seqdetective.mapping_rate.mate1" DOUBLE, "seqdetective.mapping_rate.mate2" DOUBLE, "seqdetective.nofeature_rate.mate1" DOUBLE, "seqdetective.nofeature_rate.mate2" DOUBLE, "seqdetective.sparsity.mate1" DOUBLE, "seqdetective.sparsity.mate2" DOUBLE, "seqdetective.pos_strand_rate.mate1" DOUBLE, "seqdetective.pos_strand_rate.mate2" DOUBLE, "seqdetective.readlen.mate1" BIGINT, "seqdetective.readlen.mate2" BIGINT, "seqdetective.judgement.mate1" VARCHAR, "seqdetective.judgement.mate2" VARCHAR, "seqdetective.judgement.reason" VARCHAR, platform_family VARCHAR, instrument_generation VARCHAR, read_bias VARCHAR, selection_class VARCHAR, prep_kit VARCHAR, sc_or_bulk VARCHAR, tech_class VARCHAR, technology VARCHAR, tech_variant VARCHAR, "submission.bioprojectsource.country" VARCHAR, earliest_date DATE, devstage_curation VARCHAR, devstage_curation_coarse VARCHAR, tissue_curation VARCHAR, tissue_curation_coarse VARCHAR);;
CREATE INDEX idx_run_bioproject ON run_metadata(bioproject);;
CREATE INDEX idx_run_run_accession ON run_metadata("run.accession");;