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run_metadata

129 rows where devstage_curation_coarse = "Larval" and tissue_curation_coarse = "Cell Line"

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technology 2

experiment.platform 2

experiment.library_source 2

experiment.library_selection 2

experiment.library_layout 2

tissue_curation_coarse 1

  • Cell Line · 129 ✖

tissue_curation 1

experiment.library_strategy 1

devstage_curation_coarse 1

  • Larval · 129 ✖

devstage_curation 1

Link rowid ▼ run.accession experiment.accession sample.accession study.accession bioproject study.title study.alias study.type study.abstract study.attributes study.PMIDs sample.description sample.title sample.alias sample.centername sample.attributes GEOsample.title GEOsample.dataprocessing GEOsample.source GEOsample.treatmentprotocol GEOsample.extractprotocol GEOsample.growthprotocol GEOsample.characteristics GEOsample.accession experiment.title experiment.alias experiment.library_name experiment.design_description experiment.library_construction_protocol experiment.attributes experiment.library_strategy experiment.library_source experiment.library_selection experiment.library_layout experiment.platform experiment.instrument_model experiment.spot_descriptor experiment.study_ref run.title run.attributes run.filename run.semantic_name run.total_bases run.total_spots run.alias run.read_lengths run.base_counts run.r1_length run.r2_length run.r3_length run.r4_length run.Acount run.Ccount run.Gcount run.Tcount run.Ncount run.experiment run.pool_member submission.accession submission.srasource submission.bioprojectsource seqdetective.n_mates seqdetective.mapping_rate.mate1 seqdetective.mapping_rate.mate2 seqdetective.nofeature_rate.mate1 seqdetective.nofeature_rate.mate2 seqdetective.sparsity.mate1 seqdetective.sparsity.mate2 seqdetective.pos_strand_rate.mate1 seqdetective.pos_strand_rate.mate2 seqdetective.readlen.mate1 seqdetective.readlen.mate2 seqdetective.judgement.mate1 seqdetective.judgement.mate2 seqdetective.judgement.reason platform_family instrument_generation read_bias selection_class prep_kit sc_or_bulk tech_class technology tech_variant submission.bioprojectsource.country earliest_date devstage_curation devstage_curation_coarse tissue_curation tissue_curation_coarse
10451 10451 ERR9267483 ERX8809906 ERS10996035 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393544 University of Heidelberg Compound:Acetamiprid|Concentration:100.0 µM|ENA first public:2024 03 15|Exposure time:120 hpf Id:SAMEA13393544|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:02Z|INSDC status:public|Submitter Id:S TOXR1835:S ACE 100 1|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S ACE 100 1|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S ACE 100 1 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_ACE_100_1.fastq.gz fastq 75873650.0 1517473.0 S TOXR1835:S ACE 100 1 r 0:50 A:15822311;C:19986026;G:18697821;T:21366861;N:631 50       15822311 19986026 18697821 21366861 631 ERX8809906 ERS10996035 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Larval Larval Cell Line Cell Line
10453 10453 ERR9267471 ERX8809894 ERS10996023 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393532 University of Heidelberg Compound:Imidacloprid|Concentration:25.0 µM|ENA first public:2024 03 15|Exposure time:120 hpf Id:SAMEA13393532|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:02Z|INSDC status:public|Submitter Id:S TOXR1835:S IMI 25 2|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S IMI 25 2|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S IMI 25 2 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_IMI_25_2.fastq.gz fastq 67764800.0 1355296.0 S TOXR1835:S IMI 25 2 r 0:50 A:14102811;C:17769500;G:16248227;T:19643740;N:522 50       14102811 17769500 16248227 19643740 522 ERX8809894 ERS10996023 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Larval Larval Cell Line Cell Line
10455 10455 ERR9267486 ERX8809909 ERS10996038 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393547 University of Heidelberg Compound:Acetamiprid|Concentration:12.5 µM|ENA first public:2024 03 15|Exposure time:120 hpf Id:SAMEA13393547|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:02Z|INSDC status:public|Submitter Id:S TOXR1835:S ACE 12 5 1|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S ACE 12 5 1|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S ACE 12 5 1 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_ACE_12-5_1.fastq.gz fastq 72678600.0 1453572.0 S TOXR1835:S ACE 12 5 1 r 0:50 A:15447893;C:19260513;G:17908995;T:20060590;N:609 50       15447893 19260513 17908995 20060590 609 ERX8809909 ERS10996038 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Larval Larval Cell Line Cell Line
10456 10456 ERR9267492 ERX8809915 ERS10996044 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393553 University of Heidelberg Compound:Acetamiprid|Concentration:25.0 µM|ENA first public:2024 03 15|Exposure time:120 hpf Id:SAMEA13393553|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:02Z|INSDC status:public|Submitter Id:S TOXR1835:S ACE 25 2|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S ACE 25 2|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S ACE 25 2 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_ACE_25_2.fastq.gz fastq 81198250.0 1623965.0 S TOXR1835:S ACE 25 2 r 0:50 A:17464288;C:21969971;G:19598602;T:22164717;N:672 50       17464288 21969971 19598602 22164717 672 ERX8809915 ERS10996044 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Larval Larval Cell Line Cell Line
10457 10457 ERR9267481 ERX8809904 ERS10996033 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393542 University of Heidelberg Compound:H2O|Concentration:nan nan|ENA first public:2024 03 15|Exposure time:120 hpf Id:SAMEA13393542|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:02Z|INSDC status:public|Submitter Id:S TOXR1835:S IMI NC 3|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S IMI NC 3|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S IMI NC 3 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_IMI_NC_3.fastq.gz fastq 70746600.0 1414932.0 S TOXR1835:S IMI NC 3 r 0:50 A:14872599;C:19116160;G:17308853;T:19448415;N:573 50       14872599 19116160 17308853 19448415 573 ERX8809904 ERS10996033 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Larval Larval Cell Line Cell Line
10459 10459 ERR9267466 ERX8809889 ERS10996018 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393527 University of Heidelberg Compound:Imidacloprid|Concentration:6.25 µM|ENA first public:2024 03 15|Exposure time:120 hpf Id:SAMEA13393527|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:02Z|INSDC status:public|Submitter Id:S TOXR1835:S IMI 6 5 1|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S IMI 6 5 1|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S IMI 6 5 1 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_IMI_6-5_1.fastq.gz fastq 71364750.0 1427295.0 S TOXR1835:S IMI 6 5 1 r 0:50 A:14865004;C:18778363;G:18232714;T:19488125;N:544 50       14865004 18778363 18232714 19488125 544 ERX8809889 ERS10996018 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Larval Larval Cell Line Cell Line
10460 10460 ERR9267460 ERX8809883 ERS10996012 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393521 University of Heidelberg Compound:H2O|Concentration:nan nan|ENA first public:2024 03 15|Exposure time:120 hpf Id:SAMEA13393521|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:02Z|INSDC status:public|Submitter Id:S TOXR1835:S NIC NC 3|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S NIC NC 3|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S NIC NC 3 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_NIC_NC_3.fastq.gz fastq 75658150.0 1513163.0 S TOXR1835:S NIC NC 3 r 0:50 A:15731441;C:20165272;G:18503142;T:21257746;N:549 50       15731441 20165272 18503142 21257746 549 ERX8809883 ERS10996012 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Larval Larval Cell Line Cell Line
10462 10462 ERR9267463 ERX8809886 ERS10996015 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393524 University of Heidelberg Compound:Imidacloprid|Concentration:50.0 µM|ENA first public:2024 03 15|Exposure time:120 hpf Id:SAMEA13393524|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:02Z|INSDC status:public|Submitter Id:S TOXR1835:S IMI 50 1|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S IMI 50 1|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S IMI 50 1 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_IMI_50_1.fastq.gz fastq 67890100.0 1357802.0 S TOXR1835:S IMI 50 1 r 0:50 A:14787562;C:18430411;G:17548048;T:17123490;N:589 50       14787562 18430411 17548048 17123490 589 ERX8809886 ERS10996015 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Larval Larval Cell Line Cell Line
10465 10465 ERR9267464 ERX8809887 ERS10996016 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393525 University of Heidelberg Compound:Imidacloprid|Concentration:25.0 µM|ENA first public:2024 03 15|Exposure time:120 hpf Id:SAMEA13393525|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:02Z|INSDC status:public|Submitter Id:S TOXR1835:S IMI 25 1|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S IMI 25 1|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S IMI 25 1 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_IMI_25_1.fastq.gz fastq 63903600.0 1278072.0 S TOXR1835:S IMI 25 1 r 0:50 A:13800527;C:16722813;G:16296733;T:17083017;N:510 50       13800527 16722813 16296733 17083017 510 ERX8809887 ERS10996016 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Larval Larval Cell Line Cell Line
10467 10467 ERR9267441 ERX8809864 ERS10995993 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393502 University of Heidelberg Compound:Nicotine|Concentration:100.0 µM|ENA first public:2024 03 15|Exposure time:120 hpf Id:SAMEA13393502|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:01Z|INSDC status:public|Submitter Id:S TOXR1835:S NIC 100 1|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S NIC 100 1|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S NIC 100 1 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_NIC_100_1.fastq.gz fastq 85227550.0 1704551.0 S TOXR1835:S NIC 100 1 r 0:50 A:17910834;C:22622379;G:20997857;T:23695703;N:777 50       17910834 22622379 20997857 23695703 777 ERX8809864 ERS10995993 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Larval Larval Cell Line Cell Line
10474 10474 ERR9267490 ERX8809913 ERS10996042 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393551 University of Heidelberg Compound:Acetamiprid|Concentration:100.0 µM|ENA first public:2024 03 15|Exposure time:120 hpf Id:SAMEA13393551|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:02Z|INSDC status:public|Submitter Id:S TOXR1835:S ACE 100 2|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S ACE 100 2|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S ACE 100 2 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_ACE_100_2.fastq.gz fastq 70933350.0 1418667.0 S TOXR1835:S ACE 100 2 r 0:50 A:15173580;C:19024325;G:16884416;T:19850461;N:568 50       15173580 19024325 16884416 19850461 568 ERX8809913 ERS10996042 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Larval Larval Cell Line Cell Line
10475 10475 ERR9267502 ERX8809925 ERS10996054 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393563 University of Heidelberg Compound:H2O|Concentration:nan nan|ENA first public:2024 03 15|Exposure time:120 hpf Id:SAMEA13393563|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:03Z|INSDC status:public|Submitter Id:S TOXR1835:S ACE NC 3|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S ACE NC 3|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S ACE NC 3 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_ACE_NC_3.fastq.gz fastq 79228750.0 1584575.0 S TOXR1835:S ACE NC 3 r 0:50 A:16695950;C:21491641;G:19308503;T:21731956;N:700 50       16695950 21491641 19308503 21731956 700 ERX8809925 ERS10996054 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Larval Larval Cell Line Cell Line
10477 10477 ERR9267484 ERX8809907 ERS10996036 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393545 University of Heidelberg Compound:Acetamiprid|Concentration:50.0 µM|ENA first public:2024 03 15|Exposure time:120 hpf Id:SAMEA13393545|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:02Z|INSDC status:public|Submitter Id:S TOXR1835:S ACE 50 1|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S ACE 50 1|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S ACE 50 1 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_ACE_50_1.fastq.gz fastq 71947950.0 1438959.0 S TOXR1835:S ACE 50 1 r 0:50 A:15192270;C:19017280;G:17169939;T:20567877;N:584 50       15192270 19017280 17169939 20567877 584 ERX8809907 ERS10996036 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Larval Larval Cell Line Cell Line
10481 10481 ERR9267489 ERX8809912 ERS10996041 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393550 University of Heidelberg Compound:DMSO|Concentration:0.1 %|ENA first public:2024 03 15|Exposure time:120 hpf Id:SAMEA13393550|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:02Z|INSDC status:public|Submitter Id:S TOXR1835:S ACE SC 1|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S ACE SC 1|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S ACE SC 1 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_ACE_SC_1.fastq.gz fastq 89151700.0 1783034.0 S TOXR1835:S ACE SC 1 r 0:50 A:18722635;C:23706906;G:21671705;T:25049735;N:719 50       18722635 23706906 21671705 25049735 719 ERX8809912 ERS10996041 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Larval Larval Cell Line Cell Line
10482 10482 ERR9267482 ERX8809905 ERS10996034 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393543 University of Heidelberg Compound:DMSO|Concentration:0.1 %|ENA first public:2024 03 15|Exposure time:120 hpf Id:SAMEA13393543|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:02Z|INSDC status:public|Submitter Id:S TOXR1835:S IMI SC 3|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S IMI SC 3|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S IMI SC 3 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_IMI_SC_3.fastq.gz fastq 68336650.0 1366733.0 S TOXR1835:S IMI SC 3 r 0:50 A:14352395;C:18368277;G:16626923;T:18988542;N:513 50       14352395 18368277 16626923 18988542 513 ERX8809905 ERS10996034 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Larval Larval Cell Line Cell Line
10485 10485 ERR9267450 ERX8809873 ERS10996002 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393511 University of Heidelberg Compound:Nicotine|Concentration:25.0 µM|ENA first public:2024 03 15|Exposure time:120 hpf Id:SAMEA13393511|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:01Z|INSDC status:public|Submitter Id:S TOXR1835:S NIC 25 2|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S NIC 25 2|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S NIC 25 2 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_NIC_25_2.fastq.gz fastq 81787750.0 1635755.0 S TOXR1835:S NIC 25 2 r 0:50 A:17195384;C:21754092;G:20071887;T:22765703;N:684 50       17195384 21754092 20071887 22765703 684 ERX8809873 ERS10996002 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Larval Larval Cell Line Cell Line
10488 10488 ERR9267488 ERX8809911 ERS10996040 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393549 University of Heidelberg Compound:H2O|Concentration:nan nan|ENA first public:2024 03 15|Exposure time:120 hpf Id:SAMEA13393549|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:02Z|INSDC status:public|Submitter Id:S TOXR1835:S ACE NC 1|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S ACE NC 1|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S ACE NC 1 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_ACE_NC_1.fastq.gz fastq 80984750.0 1619695.0 S TOXR1835:S ACE NC 1 r 0:50 A:16898198;C:21725890;G:19875891;T:22484095;N:676 50       16898198 21725890 19875891 22484095 676 ERX8809911 ERS10996040 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Larval Larval Cell Line Cell Line
10493 10493 ERR9267478 ERX8809901 ERS10996030 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393539 University of Heidelberg Compound:Imidacloprid|Concentration:25.0 µM|ENA first public:2024 03 15|Exposure time:120 hpf Id:SAMEA13393539|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:02Z|INSDC status:public|Submitter Id:S TOXR1835:S IMI 25 3|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S IMI 25 3|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S IMI 25 3 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_IMI_25_3.fastq.gz fastq 70097650.0 1401953.0 S TOXR1835:S IMI 25 3 r 0:50 A:14719939;C:18560782;G:16888153;T:19928203;N:573 50       14719939 18560782 16888153 19928203 573 ERX8809901 ERS10996030 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Larval Larval Cell Line Cell Line
10495 10495 ERR9267454 ERX8809877 ERS10996006 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393515 University of Heidelberg Compound:DMSO|Concentration:0.1 %|ENA first public:2024 03 15|Exposure time:120 hpf Id:SAMEA13393515|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:01Z|INSDC status:public|Submitter Id:S TOXR1835:S NIC SC 2|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S NIC SC 2|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S NIC SC 2 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_NIC_SC_2.fastq.gz fastq 78738650.0 1574773.0 S TOXR1835:S NIC SC 2 r 0:50 A:16478393;C:20748483;G:19468692;T:22042490;N:592 50       16478393 20748483 19468692 22042490 592 ERX8809877 ERS10996006 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Larval Larval Cell Line Cell Line
10498 10498 ERR9267493 ERX8809916 ERS10996045 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393554 University of Heidelberg Compound:Acetamiprid|Concentration:12.5 µM|ENA first public:2024 03 15|Exposure time:120 hpf Id:SAMEA13393554|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:03Z|INSDC status:public|Submitter Id:S TOXR1835:S ACE 12 5 2|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S ACE 12 5 2|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S ACE 12 5 2 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_ACE_12-5_2.fastq.gz fastq 79261850.0 1585237.0 S TOXR1835:S ACE 12 5 2 r 0:50 A:17091078;C:21794461;G:19064493;T:21311173;N:645 50       17091078 21794461 19064493 21311173 645 ERX8809916 ERS10996045 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Larval Larval Cell Line Cell Line
10506 10506 ERR9267472 ERX8809895 ERS10996024 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393533 University of Heidelberg Compound:Imidacloprid|Concentration:12.5 µM|ENA first public:2024 03 15|Exposure time:120 hpf Id:SAMEA13393533|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:02Z|INSDC status:public|Submitter Id:S TOXR1835:S IMI 12 5 2|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S IMI 12 5 2|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S IMI 12 5 2 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_IMI_12-5_2.fastq.gz fastq 62605950.0 1252119.0 S TOXR1835:S IMI 12 5 2 r 0:50 A:13198703;C:16322295;G:15337506;T:17746921;N:525 50       13198703 16322295 15337506 17746921 525 ERX8809895 ERS10996024 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Larval Larval Cell Line Cell Line
10509 10509 ERR9267497 ERX8809920 ERS10996049 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393558 University of Heidelberg Compound:Acetamiprid|Concentration:100.0 µM|ENA first public:2024 03 15|Exposure time:120 hpf Id:SAMEA13393558|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:03Z|INSDC status:public|Submitter Id:S TOXR1835:S ACE 100 3|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S ACE 100 3|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S ACE 100 3 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_ACE_100_3.fastq.gz fastq 72002200.0 1440044.0 S TOXR1835:S ACE 100 3 r 0:50 A:15120122;C:19004999;G:17634421;T:20242017;N:641 50       15120122 19004999 17634421 20242017 641 ERX8809920 ERS10996049 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Larval Larval Cell Line Cell Line
10510 10510 ERR9267474 ERX8809897 ERS10996026 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393535 University of Heidelberg Compound:H2O|Concentration:nan nan|ENA first public:2024 03 15|Exposure time:120 hpf Id:SAMEA13393535|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:02Z|INSDC status:public|Submitter Id:S TOXR1835:S IMI NC 2|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S IMI NC 2|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S IMI NC 2 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_IMI_NC_2.fastq.gz fastq 70051850.0 1401037.0 S TOXR1835:S IMI NC 2 r 0:50 A:14791722;C:18967741;G:17379694;T:18912127;N:566 50       14791722 18967741 17379694 18912127 566 ERX8809897 ERS10996026 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Larval Larval Cell Line Cell Line
10514 10514 ERR9267451 ERX8809874 ERS10996003 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393512 University of Heidelberg Compound:Nicotine|Concentration:12.5 µM|ENA first public:2024 03 15|Exposure time:120 hpf Id:SAMEA13393512|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:01Z|INSDC status:public|Submitter Id:S TOXR1835:S NIC 12 5 2|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S NIC 12 5 2|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S NIC 12 5 2 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_NIC_12-5_2.fastq.gz fastq 79086700.0 1581734.0 S TOXR1835:S NIC 12 5 2 r 0:50 A:16566697;C:21259397;G:19607165;T:21652759;N:682 50       16566697 21259397 19607165 21652759 682 ERX8809874 ERS10996003 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Larval Larval Cell Line Cell Line
10519 10519 ERR9267475 ERX8809898 ERS10996027 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393536 University of Heidelberg Compound:DMSO|Concentration:0.1 %|ENA first public:2024 03 15|Exposure time:120 hpf Id:SAMEA13393536|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:02Z|INSDC status:public|Submitter Id:S TOXR1835:S IMI SC 2|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S IMI SC 2|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S IMI SC 2 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_IMI_SC_2.fastq.gz fastq 71269750.0 1425395.0 S TOXR1835:S IMI SC 2 r 0:50 A:14990821;C:19163243;G:17680246;T:19434847;N:593 50       14990821 19163243 17680246 19434847 593 ERX8809898 ERS10996027 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Larval Larval Cell Line Cell Line
10522 10522 ERR9267469 ERX8809892 ERS10996021 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393530 University of Heidelberg Compound:Imidacloprid|Concentration:100.0 µM|ENA first public:2024 03 15|Exposure time:120 hpf Id:SAMEA13393530|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:02Z|INSDC status:public|Submitter Id:S TOXR1835:S IMI 100 2|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S IMI 100 2|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S IMI 100 2 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_IMI_100_2.fastq.gz fastq 68920850.0 1378417.0 S TOXR1835:S IMI 100 2 r 0:50 A:14765262;C:18545505;G:17050728;T:18558763;N:592 50       14765262 18545505 17050728 18558763 592 ERX8809892 ERS10996021 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Larval Larval Cell Line Cell Line
10523 10523 ERR9267491 ERX8809914 ERS10996043 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393552 University of Heidelberg Compound:Acetamiprid|Concentration:50.0 µM|ENA first public:2024 03 15|Exposure time:120 hpf Id:SAMEA13393552|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:02Z|INSDC status:public|Submitter Id:S TOXR1835:S ACE 50 2|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S ACE 50 2|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S ACE 50 2 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_ACE_50_2.fastq.gz fastq 82891350.0 1657827.0 S TOXR1835:S ACE 50 2 r 0:50 A:17780093;C:22477070;G:19709528;T:22923970;N:689 50       17780093 22477070 19709528 22923970 689 ERX8809914 ERS10996043 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Larval Larval Cell Line Cell Line
10526 10526 ERR9267442 ERX8809865 ERS10995994 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393503 University of Heidelberg Compound:Nicotine|Concentration:50.0 µM|ENA first public:2024 03 15|Exposure time:120 hpf Id:SAMEA13393503|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:01Z|INSDC status:public|Submitter Id:S TOXR1835:S NIC 50 1|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S NIC 50 1|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S NIC 50 1 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_NIC_50_1.fastq.gz fastq 86937400.0 1738748.0 S TOXR1835:S NIC 50 1 r 0:50 A:18376028;C:22955760;G:20801284;T:24803631;N:697 50       18376028 22955760 20801284 24803631 697 ERX8809865 ERS10995994 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Larval Larval Cell Line Cell Line
10527 10527 ERR9267477 ERX8809900 ERS10996029 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393538 University of Heidelberg Compound:Imidacloprid|Concentration:50.0 µM|ENA first public:2024 03 15|Exposure time:120 hpf Id:SAMEA13393538|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:02Z|INSDC status:public|Submitter Id:S TOXR1835:S IMI 50 3|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S IMI 50 3|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S IMI 50 3 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_IMI_50_3.fastq.gz fastq 68870250.0 1377405.0 S TOXR1835:S IMI 50 3 r 0:50 A:14673764;C:18662859;G:16825853;T:18707243;N:531 50       14673764 18662859 16825853 18707243 531 ERX8809900 ERS10996029 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Larval Larval Cell Line Cell Line
10528 10528 ERR9267458 ERX8809881 ERS10996010 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393519 University of Heidelberg Compound:Nicotine|Concentration:12.5 µM|ENA first public:2024 03 15|Exposure time:120 hpf Id:SAMEA13393519|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:01Z|INSDC status:public|Submitter Id:S TOXR1835:S NIC 12 5 3|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S NIC 12 5 3|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S NIC 12 5 3 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_NIC_12-5_3.fastq.gz fastq 72887050.0 1457741.0 S TOXR1835:S NIC 12 5 3 r 0:50 A:15269696;C:19618192;G:17977122;T:20021422;N:618 50       15269696 19618192 17977122 20021422 618 ERX8809881 ERS10996010 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Larval Larval Cell Line Cell Line
10531 10531 ERR9267501 ERX8809924 ERS10996053 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393562 University of Heidelberg Compound:Acetamiprid|Concentration:6.25 µM|ENA first public:2024 03 15|Exposure time:120 hpf Id:SAMEA13393562|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:03Z|INSDC status:public|Submitter Id:S TOXR1835:S ACE 6 25 3|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S ACE 6 25 3|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S ACE 6 25 3 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_ACE_6-25_3.fastq.gz fastq 78902800.0 1578056.0 S TOXR1835:S ACE 6 25 3 r 0:50 A:16635662;C:21154825;G:19282598;T:21829091;N:624 50       16635662 21154825 19282598 21829091 624 ERX8809924 ERS10996053 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Larval Larval Cell Line Cell Line
10537 10537 ERR9267473 ERX8809896 ERS10996025 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393534 University of Heidelberg Compound:Imidacloprid|Concentration:6.25 µM|ENA first public:2024 03 15|Exposure time:120 hpf Id:SAMEA13393534|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:02Z|INSDC status:public|Submitter Id:S TOXR1835:S IMI 6 5 2|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S IMI 6 5 2|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S IMI 6 5 2 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_IMI_6-5_2.fastq.gz fastq 66003850.0 1320077.0 S TOXR1835:S IMI 6 5 2 r 0:50 A:13892831;C:17145836;G:15678798;T:19285813;N:572 50       13892831 17145836 15678798 19285813 572 ERX8809896 ERS10996025 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Larval Larval Cell Line Cell Line
10538 10538 ERR9267445 ERX8809868 ERS10995997 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393506 University of Heidelberg Compound:Nicotine|Concentration:6.25 µM|ENA first public:2024 03 15|Exposure time:120 hpf Id:SAMEA13393506|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:01Z|INSDC status:public|Submitter Id:S TOXR1835:S NIC 6 25 1|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S NIC 6 25 1|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S NIC 6 25 1 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_NIC_6-25_1.fastq.gz fastq 77192900.0 1543858.0 S TOXR1835:S NIC 6 25 1 r 0:50 A:16321450;C:20332255;G:18995411;T:21543128;N:656 50       16321450 20332255 18995411 21543128 656 ERX8809868 ERS10995997 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Larval Larval Cell Line Cell Line
10541 10541 ERR9267462 ERX8809885 ERS10996014 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393523 University of Heidelberg Compound:Imidacloprid|Concentration:100.0 µM|ENA first public:2024 03 15|Exposure time:120 hpf Id:SAMEA13393523|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:02Z|INSDC status:public|Submitter Id:S TOXR1835:S IMI 100 1|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S IMI 100 1|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S IMI 100 1 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_IMI_100_1.fastq.gz fastq 67651700.0 1353034.0 S TOXR1835:S IMI 100 1 r 0:50 A:14448995;C:18337833;G:16889577;T:17974722;N:573 50       14448995 18337833 16889577 17974722 573 ERX8809885 ERS10996014 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Larval Larval Cell Line Cell Line
10558 10558 ERR9267457 ERX8809880 ERS10996009 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393518 University of Heidelberg Compound:Nicotine|Concentration:25.0 µM|ENA first public:2024 03 15|Exposure time:120 hpf Id:SAMEA13393518|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:01Z|INSDC status:public|Submitter Id:S TOXR1835:S NIC 25 3|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S NIC 25 3|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S NIC 25 3 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_NIC_25_3.fastq.gz fastq 77792250.0 1555845.0 S TOXR1835:S NIC 25 3 r 0:50 A:16159740;C:20886052;G:19115250;T:21630612;N:596 50       16159740 20886052 19115250 21630612 596 ERX8809880 ERS10996009 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Larval Larval Cell Line Cell Line
10562 10562 ERR9267498 ERX8809921 ERS10996050 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393559 University of Heidelberg Compound:Acetamiprid|Concentration:50.0 µM|ENA first public:2024 03 15|Exposure time:120 hpf Id:SAMEA13393559|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:03Z|INSDC status:public|Submitter Id:S TOXR1835:S ACE 50 3|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S ACE 50 3|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S ACE 50 3 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_ACE_50_3.fastq.gz fastq 66735100.0 1334702.0 S TOXR1835:S ACE 50 3 r 0:50 A:14090657;C:17627888;G:15987635;T:19028382;N:538 50       14090657 17627888 15987635 19028382 538 ERX8809921 ERS10996050 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Larval Larval Cell Line Cell Line
10564 10564 ERR9267499 ERX8809922 ERS10996051 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393560 University of Heidelberg Compound:Acetamiprid|Concentration:25.0 µM|ENA first public:2024 03 15|Exposure time:120 hpf Id:SAMEA13393560|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:03Z|INSDC status:public|Submitter Id:S TOXR1835:S ACE 25 3|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S ACE 25 3|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S ACE 25 3 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_ACE_25_3.fastq.gz fastq 78357000.0 1567140.0 S TOXR1835:S ACE 25 3 r 0:50 A:16290242;C:20876680;G:18749240;T:22440170;N:668 50       16290242 20876680 18749240 22440170 668 ERX8809922 ERS10996051 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Larval Larval Cell Line Cell Line
10565 10565 ERR9267495 ERX8809918 ERS10996047 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393556 University of Heidelberg Compound:H2O|Concentration:nan nan|ENA first public:2024 03 15|Exposure time:120 hpf Id:SAMEA13393556|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:03Z|INSDC status:public|Submitter Id:S TOXR1835:S ACE NC 2|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S ACE NC 2|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S ACE NC 2 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_ACE_NC_2.fastq.gz fastq 87177500.0 1743550.0 S TOXR1835:S ACE NC 2 r 0:50 A:18702838;C:23589640;G:21005326;T:23879045;N:651 50       18702838 23589640 21005326 23879045 651 ERX8809918 ERS10996047 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Larval Larval Cell Line Cell Line
10566 10566 ERR9267479 ERX8809902 ERS10996031 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393540 University of Heidelberg Compound:Imidacloprid|Concentration:12.5 µM|ENA first public:2024 03 15|Exposure time:120 hpf Id:SAMEA13393540|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:02Z|INSDC status:public|Submitter Id:S TOXR1835:S IMI 12 5 3|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S IMI 12 5 3|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S IMI 12 5 3 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_IMI_12-5_3.fastq.gz fastq 76504050.0 1530081.0 S TOXR1835:S IMI 12 5 3 r 0:50 A:15917823;C:20251636;G:18382184;T:21951812;N:595 50       15917823 20251636 18382184 21951812 595 ERX8809902 ERS10996031 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Larval Larval Cell Line Cell Line
10568 10568 ERR9267455 ERX8809878 ERS10996007 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393516 University of Heidelberg Compound:Nicotine|Concentration:100.0 µM|ENA first public:2024 03 15|Exposure time:120 hpf Id:SAMEA13393516|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:01Z|INSDC status:public|Submitter Id:S TOXR1835:S NIC 100 3|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S NIC 100 3|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S NIC 100 3 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_NIC_100_3.fastq.gz fastq 64329700.0 1286594.0 S TOXR1835:S NIC 100 3 r 0:50 A:13354537;C:17191151;G:16044852;T:17738642;N:518 50       13354537 17191151 16044852 17738642 518 ERX8809878 ERS10996007 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Larval Larval Cell Line Cell Line
10569 10569 ERR9267467 ERX8809890 ERS10996019 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393528 University of Heidelberg Compound:H2O|Concentration:nan nan|ENA first public:2024 03 15|Exposure time:120 hpf Id:SAMEA13393528|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:02Z|INSDC status:public|Submitter Id:S TOXR1835:S IMI NC 1|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S IMI NC 1|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S IMI NC 1 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_IMI_NC_1.fastq.gz fastq 71303500.0 1426070.0 S TOXR1835:S IMI NC 1 r 0:50 A:14972356;C:19465424;G:18260666;T:18604434;N:620 50       14972356 19465424 18260666 18604434 620 ERX8809890 ERS10996019 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Larval Larval Cell Line Cell Line
10574 10574 ERR9267485 ERX8809908 ERS10996037 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393546 University of Heidelberg Compound:Acetamiprid|Concentration:25.0 µM|ENA first public:2024 03 15|Exposure time:120 hpf Id:SAMEA13393546|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:02Z|INSDC status:public|Submitter Id:S TOXR1835:S ACE 25 1|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S ACE 25 1|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S ACE 25 1 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_ACE_25_1.fastq.gz fastq 80068750.0 1601375.0 S TOXR1835:S ACE 25 1 r 0:50 A:16801071;C:21302808;G:19395019;T:22569214;N:638 50       16801071 21302808 19395019 22569214 638 ERX8809908 ERS10996037 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Larval Larval Cell Line Cell Line
10578 10578 ERR9267461 ERX8809884 ERS10996013 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393522 University of Heidelberg Compound:DMSO|Concentration:0.1 %|ENA first public:2024 03 15|Exposure time:120 hpf Id:SAMEA13393522|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:02Z|INSDC status:public|Submitter Id:S TOXR1835:S NIC SC 3|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S NIC SC 3|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S NIC SC 3 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_NIC_SC_3.fastq.gz fastq 69671500.0 1393430.0 S TOXR1835:S NIC SC 3 r 0:50 A:14663517;C:18563157;G:16756431;T:19687795;N:600 50       14663517 18563157 16756431 19687795 600 ERX8809884 ERS10996013 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Larval Larval Cell Line Cell Line
10579 10579 ERR9267452 ERX8809875 ERS10996004 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393513 University of Heidelberg Compound:Nicotine|Concentration:6.25 µM|ENA first public:2024 03 15|Exposure time:120 hpf Id:SAMEA13393513|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:01Z|INSDC status:public|Submitter Id:S TOXR1835:S NIC 6 25 2|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S NIC 6 25 2|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S NIC 6 25 2 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_NIC_6-25_2.fastq.gz fastq 72228350.0 1444567.0 S TOXR1835:S NIC 6 25 2 r 0:50 A:15269105;C:19470263;G:17939145;T:19549252;N:585 50       15269105 19470263 17939145 19549252 585 ERX8809875 ERS10996004 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Larval Larval Cell Line Cell Line
10581 10581 ERR9267459 ERX8809882 ERS10996011 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       S TOXR1835:S NIC 6 25 3   Treatment:repeated dose|Treatment scheme:daily|organism:Danio rerio|cell line:null|Compound:Nicotine|scientific name:Danio rerio|Concentration:6.25 µM|Exposure time:120 hpf name:zebrafish|ENA LAST UPDATE:2022 03 15                 Illumina HiSeq 2500 sequencing S TOXR1835:S NIC 6 25 3 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_NIC_6-25_3.fastq.gz fastq 65918850.0 1318377.0 S TOXR1835:S NIC 6 25 3 r 0:50 A:13790777;C:17684184;G:16201510;T:18241796;N:583 50       13790777 17684184 16201510 18241796 583 ERX8809882 ERS10996011 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Larval Larval Cell Line Cell Line
10582 10582 ERR9267500 ERX8809923 ERS10996052 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393561 University of Heidelberg Compound:Acetamiprid|Concentration:12.5 µM|ENA first public:2024 03 15|Exposure time:120 hpf Id:SAMEA13393561|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:03Z|INSDC status:public|Submitter Id:S TOXR1835:S ACE 12 5 3|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S ACE 12 5 3|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S ACE 12 5 3 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_ACE_12-5_3.fastq.gz fastq 81412850.0 1628257.0 S TOXR1835:S ACE 12 5 3 r 0:50 A:17597149;C:21648822;G:19803766;T:22362492;N:621 50       17597149 21648822 19803766 22362492 621 ERX8809923 ERS10996052 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Larval Larval Cell Line Cell Line
10583 10583 ERR9267496 ERX8809919 ERS10996048 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393557 University of Heidelberg Compound:DMSO|Concentration:0.1 %|ENA first public:2024 03 15|Exposure time:120 hpf Id:SAMEA13393557|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:03Z|INSDC status:public|Submitter Id:S TOXR1835:S ACE SC 2|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S ACE SC 2|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S ACE SC 2 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_ACE_SC_2.fastq.gz fastq 76563150.0 1531263.0 S TOXR1835:S ACE SC 2 r 0:50 A:16339030;C:20626366;G:18608055;T:20989083;N:616 50       16339030 20626366 18608055 20989083 616 ERX8809919 ERS10996048 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Larval Larval Cell Line Cell Line
10585 10585 ERR9267487 ERX8809910 ERS10996039 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393548 University of Heidelberg Compound:Acetamiprid|Concentration:6.25 µM|ENA first public:2024 03 15|Exposure time:120 hpf Id:SAMEA13393548|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:02Z|INSDC status:public|Submitter Id:S TOXR1835:S ACE 6 25 1|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S ACE 6 25 1|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S ACE 6 25 1 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_ACE_6-25_1.fastq.gz fastq 74822950.0 1496459.0 S TOXR1835:S ACE 6 25 1 r 0:50 A:15683965;C:19998316;G:18403565;T:20736408;N:696 50       15683965 19998316 18403565 20736408 696 ERX8809910 ERS10996039 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Larval Larval Cell Line Cell Line
10588 10588 ERR9267443 ERX8809866 ERS10995995 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393504 University of Heidelberg Compound:Nicotine|Concentration:25.0 µM|ENA first public:2024 03 15|Exposure time:120 hpf Id:SAMEA13393504|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:01Z|INSDC status:public|Submitter Id:S TOXR1835:S NIC 25 1|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S NIC 25 1|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S NIC 25 1 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_NIC_25_1.fastq.gz fastq 324700.0 6494.0 S TOXR1835:S NIC 25 1 r 0:50 A:81385;C:82453;G:73698;T:87162;N:2 50       81385 82453 73698 87162 2 ERX8809866 ERS10995995 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Larval Larval Cell Line Cell Line
10591 10591 ERR9267446 ERX8809869 ERS10995998 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393507 University of Heidelberg Compound:H2O|Concentration:nan nan|ENA first public:2024 03 15|Exposure time:120 hpf Id:SAMEA13393507|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:01Z|INSDC status:public|Submitter Id:S TOXR1835:S NIC NC 1|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S NIC NC 1|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S NIC NC 1 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_NIC_NC_1.fastq.gz fastq 78776500.0 1575530.0 S TOXR1835:S NIC NC 1 r 0:50 A:16692329;C:20712558;G:19252179;T:22118843;N:591 50       16692329 20712558 19252179 22118843 591 ERX8809869 ERS10995998 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Larval Larval Cell Line Cell Line
10592 10592 ERR9267468 ERX8809891 ERS10996020 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393529 University of Heidelberg Compound:DMSO|Concentration:0.1 %|ENA first public:2024 03 15|Exposure time:120 hpf Id:SAMEA13393529|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:02Z|INSDC status:public|Submitter Id:S TOXR1835:S IMI SC 1|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S IMI SC 1|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S IMI SC 1 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_IMI_SC_1.fastq.gz fastq 68194700.0 1363894.0 S TOXR1835:S IMI SC 1 r 0:50 A:14578258;C:18626744;G:17309886;T:17679240;N:572 50       14578258 18626744 17309886 17679240 572 ERX8809891 ERS10996020 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Larval Larval Cell Line Cell Line
10593 10593 ERR9267444 ERX8809867 ERS10995996 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393505 University of Heidelberg Compound:Nicotine|Concentration:12.5 µM|ENA first public:2024 03 15|Exposure time:120 hpf Id:SAMEA13393505|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:01Z|INSDC status:public|Submitter Id:S TOXR1835:S NIC 12 5 1|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S NIC 12 5 1|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S NIC 12 5 1 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_NIC_12-5_1.fastq.gz fastq 79478650.0 1589573.0 S TOXR1835:S NIC 12 5 1 r 0:50 A:16831242;C:21102248;G:19489238;T:22055257;N:665 50       16831242 21102248 19489238 22055257 665 ERX8809867 ERS10995996 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Larval Larval Cell Line Cell Line
10596 10596 ERR9267465 ERX8809888 ERS10996017 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393526 University of Heidelberg Compound:Imidacloprid|Concentration:12.5 µM|ENA first public:2024 03 15|Exposure time:120 hpf Id:SAMEA13393526|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:02Z|INSDC status:public|Submitter Id:S TOXR1835:S IMI 12 5 1|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S IMI 12 5 1|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S IMI 12 5 1 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_IMI_12-5_1.fastq.gz fastq 1861450.0 37229.0 S TOXR1835:S IMI 12 5 1 r 0:50 A:398400;C:455282;G:485017;T:522737;N:14 50       398400 455282 485017 522737 14 ERX8809888 ERS10996017 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Larval Larval Cell Line Cell Line
10599 10599 ERR9267447 ERX8809870 ERS10995999 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393508 University of Heidelberg Compound:DMSO|Concentration:0.1 %|ENA first public:2024 03 15|Exposure time:120 hpf Id:SAMEA13393508|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:01Z|INSDC status:public|Submitter Id:S TOXR1835:S NIC SC 1|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S NIC SC 1|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S NIC SC 1 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_NIC_SC_1.fastq.gz fastq 69798550.0 1395971.0 S TOXR1835:S NIC SC 1 r 0:50 A:14876438;C:18453836;G:16881899;T:19585818;N:559 50       14876438 18453836 16881899 19585818 559 ERX8809870 ERS10995999 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Larval Larval Cell Line Cell Line
10600 10600 ERR9267470 ERX8809893 ERS10996022 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393531 University of Heidelberg Compound:Imidacloprid|Concentration:50.0 µM|ENA first public:2024 03 15|Exposure time:120 hpf Id:SAMEA13393531|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:02Z|INSDC status:public|Submitter Id:S TOXR1835:S IMI 50 2|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S IMI 50 2|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S IMI 50 2 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_IMI_50_2.fastq.gz fastq 61374100.0 1227482.0 S TOXR1835:S IMI 50 2 r 0:50 A:13120883;C:16048881;G:14869892;T:17333950;N:494 50       13120883 16048881 14869892 17333950 494 ERX8809893 ERS10996022 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Larval Larval Cell Line Cell Line
10603 10603 ERR9267494 ERX8809917 ERS10996046 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       S TOXR1835:S ACE 6 25 2   Treatment:repeated dose|Treatment scheme:daily|organism:Danio rerio|cell line:null|Compound:Acetamiprid|scientific name:Danio rerio|Concentration:6.25 µM|Exposure time:120 hpf name:zebrafish|ENA LAST UPDATE:2022 03 15                 Illumina HiSeq 2500 sequencing S TOXR1835:S ACE 6 25 2 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_ACE_6-25_2.fastq.gz fastq 77603400.0 1552068.0 S TOXR1835:S ACE 6 25 2 r 0:50 A:16627878;C:20898064;G:18807419;T:21269347;N:692 50       16627878 20898064 18807419 21269347 692 ERX8809917 ERS10996046 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Larval Larval Cell Line Cell Line
10605 10605 ERR9267456 ERX8809879 ERS10996008 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393517 University of Heidelberg Compound:Nicotine|Concentration:50.0 µM|ENA first public:2024 03 15|Exposure time:120 hpf Id:SAMEA13393517|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:01Z|INSDC status:public|Submitter Id:S TOXR1835:S NIC 50 3|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S NIC 50 3|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S NIC 50 3 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_NIC_50_3.fastq.gz fastq 84242750.0 1684855.0 S TOXR1835:S NIC 50 3 r 0:50 A:17866389;C:22489502;G:20745003;T:23141161;N:695 50       17866389 22489502 20745003 23141161 695 ERX8809879 ERS10996008 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Larval Larval Cell Line Cell Line
10606 10606 ERR9267448 ERX8809871 ERS10996000 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393509 University of Heidelberg Compound:Nicotine|Concentration:100.0 µM|ENA first public:2024 03 15|Exposure time:120 hpf Id:SAMEA13393509|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:01Z|INSDC status:public|Submitter Id:S TOXR1835:S NIC 100 2|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S NIC 100 2|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S NIC 100 2 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_NIC_100_2.fastq.gz fastq 67597350.0 1351947.0 S TOXR1835:S NIC 100 2 r 0:50 A:14133647;C:17986780;G:16716362;T:18760014;N:547 50       14133647 17986780 16716362 18760014 547 ERX8809871 ERS10996000 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Larval Larval Cell Line Cell Line
10607 10607 ERR9267453 ERX8809876 ERS10996005 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393514 University of Heidelberg Compound:H2O|Concentration:nan nan|ENA first public:2024 03 15|Exposure time:120 hpf Id:SAMEA13393514|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:01Z|INSDC status:public|Submitter Id:S TOXR1835:S NIC NC 2|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S NIC NC 2|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S NIC NC 2 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_NIC_NC_2.fastq.gz fastq 88602950.0 1772059.0 S TOXR1835:S NIC NC 2 r 0:50 A:18502074;C:23760625;G:21777996;T:24561542;N:713 50       18502074 23760625 21777996 24561542 713 ERX8809876 ERS10996005 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Larval Larval Cell Line Cell Line
10610 10610 ERR9267476 ERX8809899 ERS10996028 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       S TOXR1835:S IMI 100 3   Treatment:repeated dose|Treatment scheme:daily|organism:Danio rerio|cell line:null|Compound:Imidacloprid|scientific name:Danio rerio|Concentration:100.0 µM|Exposure time:120 hpf name:zebrafish|ENA LAST UPDATE:2022 03 15                 Illumina HiSeq 2500 sequencing S TOXR1835:S IMI 100 3 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_IMI_100_3.fastq.gz fastq 76147550.0 1522951.0 S TOXR1835:S IMI 100 3 r 0:50 A:15973205;C:20261271;G:18450496;T:21462008;N:570 50       15973205 20261271 18450496 21462008 570 ERX8809899 ERS10996028 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Larval Larval Cell Line Cell Line
10616 10616 ERR9267449 ERX8809872 ERS10996001 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       S TOXR1835:S NIC 50 2   Treatment:repeated dose|Treatment scheme:daily|organism:Danio rerio|cell line:null|Compound:Nicotine|scientific name:Danio rerio|Concentration:50.0 µM|Exposure time:120 hpf name:zebrafish|ENA LAST UPDATE:2022 03 15                 Illumina HiSeq 2500 sequencing S TOXR1835:S NIC 50 2 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_NIC_50_2.fastq.gz fastq 82035000.0 1640700.0 S TOXR1835:S NIC 50 2 r 0:50 A:17111461;C:21971674;G:20445991;T:22505218;N:656 50       17111461 21971674 20445991 22505218 656 ERX8809872 ERS10996001 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Larval Larval Cell Line Cell Line
10619 10619 ERR9267503 ERX8809926 ERS10996055 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       S TOXR1835:S ACE SC 3   Treatment:repeated dose|Treatment scheme:daily|organism:Danio rerio|cell line:null|Compound:DMSO|scientific name:Danio rerio|Concentration:0.1 %|Exposure time:120 hpf name:zebrafish|ENA LAST UPDATE:2022 03 15                 Illumina HiSeq 2500 sequencing S TOXR1835:S ACE SC 3 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_ACE_SC_3.fastq.gz fastq 75607750.0 1512155.0 S TOXR1835:S ACE SC 3 r 0:50 A:16012201;C:20353728;G:18357046;T:20884151;N:624 50       16012201 20353728 18357046 20884151 624 ERX8809926 ERS10996055 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Larval Larval Cell Line Cell Line
10621 10621 ERR9267480 ERX8809903 ERS10996032 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       S TOXR1835:S IMI 6 5 3   Treatment:repeated dose|Treatment scheme:daily|organism:Danio rerio|cell line:null|Compound:Imidacloprid|scientific name:Danio rerio|Concentration:6.25 µM|Exposure time:120 hpf name:zebrafish|ENA LAST UPDATE:2022 03 15                 Illumina HiSeq 2500 sequencing S TOXR1835:S IMI 6 5 3 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_IMI_6-5_3.fastq.gz fastq 74619550.0 1492391.0 S TOXR1835:S IMI 6 5 3 r 0:50 A:15586305;C:19698092;G:17667657;T:21666833;N:663 50       15586305 19698092 17667657 21666833 663 ERX8809903 ERS10996032 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Larval Larval Cell Line Cell Line
10626 10626 ERR9269923 ERX8812320 ERS10998387 ERP136288 PRJEB51641 CS2 UHEI DART 96 120h raw data EUT053 S-TOXR1832 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13395732 University of Heidelberg Compound:Par1|Concentration:125.0 µM|ENA first public:2024 03 15|Exposure time:96 hpf|External Id:SAMEA13395732|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:53:12Z|INSDC status:public|Submitter Id:S TOXR1832:S 96hpf 125 Par1 UHEI|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1832:S 96hpf 125 Par1 UHEI|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1832:S 96hpf 125 Par1 UHEI e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136288 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_96hpf_125_Par1_UHEI.fastq.gz fastq 86175300.0 1723506.0 S TOXR1832:S 96hpf 125 Par1 UHEI r 0:50 A:18321147;C:22492449;G:19992098;T:25358176;N:11430 50       18321147 22492449 19992098 25358176 11430 ERX8812320 ERS10998387 ERA10091710 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Larval Larval Cell Line Cell Line
10627 10627 ERR9269909 ERX8812306 ERS10998372 ERP136288 PRJEB51641 CS2 UHEI DART 96 120h raw data EUT053 S-TOXR1832 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13395718 University of Heidelberg Compound:VPA|Concentration:200.0 µM|ENA first public:2024 03 15|Exposure time:120 hpf|External Id:SAMEA13395718|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:53:12Z|INSDC status:public|Submitter Id:S TOXR1832:S 120hpf VPA200 UHEI|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1832:S 120hpf VPA200 UHEI|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1832:S 120hpf VPA200 UHEI e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136288 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_120hpf_VPA200_UHEI.fastq.gz fastq 90865900.0 1817318.0 S TOXR1832:S 120hpf VPA200 UHEI r 0:50 A:19124895;C:23202905;G:21145843;T:27380007;N:12250 50       19124895 23202905 21145843 27380007 12250 ERX8812306 ERS10998372 ERA10091710 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Larval Larval Cell Line Cell Line
10629 10629 ERR9269922 ERX8812319 ERS10998386 ERP136288 PRJEB51641 CS2 UHEI DART 96 120h raw data EUT053 S-TOXR1832 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13395731 University of Heidelberg Compound:H2O|Concentration:nan nan|ENA first public:2024 03 15|Exposure time:96 hpf|External Id:SAMEA13395731|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:53:12Z|INSDC status:public|Submitter Id:S TOXR1832:S 96hpf NC Par1 UHEI|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1832:S 96hpf NC Par1 UHEI|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1832:S 96hpf NC Par1 UHEI e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136288 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_96hpf_NC_Par1_UHEI.fastq.gz fastq 86509150.0 1730183.0 S TOXR1832:S 96hpf NC Par1 UHEI r 0:50 A:18403565;C:22645833;G:20155841;T:25292530;N:11381 50       18403565 22645833 20155841 25292530 11381 ERX8812319 ERS10998386 ERA10091710 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Larval Larval Cell Line Cell Line
10631 10631 ERR9269924 ERX8812321 ERS10998388 ERP136288 PRJEB51641 CS2 UHEI DART 96 120h raw data EUT053 S-TOXR1832 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13395733 University of Heidelberg Compound:Par1|Concentration:500.0 µM|ENA first public:2024 03 15|Exposure time:96 hpf|External Id:SAMEA13395733|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:53:12Z|INSDC status:public|Submitter Id:S TOXR1832:S 96hpf 500 Par1 UHEI|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1832:S 96hpf 500 Par1 UHEI|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1832:S 96hpf 500 Par1 UHEI e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136288 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_96hpf_500_Par1_UHEI.fastq.gz fastq 88631900.0 1772638.0 S TOXR1832:S 96hpf 500 Par1 UHEI r 0:50 A:18744229;C:23263324;G:20626901;T:25985883;N:11563 50       18744229 23263324 20626901 25985883 11563 ERX8812321 ERS10998388 ERA10091710 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Larval Larval Cell Line Cell Line
10632 10632 ERR9269918 ERX8812315 ERS10998381 ERP136288 PRJEB51641 CS2 UHEI DART 96 120h raw data EUT053 S-TOXR1832 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13395727 University of Heidelberg Compound:VPA|Concentration:10.0 µM|ENA first public:2024 03 15|Exposure time:120 hpf|External Id:SAMEA13395727|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:53:12Z|INSDC status:public|Submitter Id:S TOXR1832:S 120hpf VPA10 UHEI|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1832:S 120hpf VPA10 UHEI|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1832:S 120hpf VPA10 UHEI e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136288 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_120hpf_VPA10_UHEI.fastq.gz fastq 90340800.0 1806816.0 S TOXR1832:S 120hpf VPA10 UHEI r 0:50 A:19390953;C:23705847;G:21212827;T:26019205;N:11968 50       19390953 23705847 21212827 26019205 11968 ERX8812315 ERS10998381 ERA10091710 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Larval Larval Cell Line Cell Line
10633 10633 ERR9269915 ERX8812312 ERS10998378 ERP136288 PRJEB51641 CS2 UHEI DART 96 120h raw data EUT053 S-TOXR1832 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13395724 University of Heidelberg Compound:VPA|Concentration:50.0 µM|ENA first public:2024 03 15|Exposure time:120 hpf|External Id:SAMEA13395724|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:53:12Z|INSDC status:public|Submitter Id:S TOXR1832:S 120hpf VPA50 UHEI|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1832:S 120hpf VPA50 UHEI|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1832:S 120hpf VPA50 UHEI e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136288 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_120hpf_VPA50_UHEI.fastq.gz fastq 101011100.0 2020222.0 S TOXR1832:S 120hpf VPA50 UHEI r 0:50 A:21594629;C:26607706;G:23991618;T:28803713;N:13434 50       21594629 26607706 23991618 28803713 13434 ERX8812312 ERS10998378 ERA10091710 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Larval Larval Cell Line Cell Line
10638 10638 ERR9269903 ERX8812300 ERS10998365 ERP136288 PRJEB51641 CS2 UHEI DART 96 120h raw data EUT053 S-TOXR1832 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13395712 University of Heidelberg Compound:H2O|Concentration:nan µM|ENA first public:2024 03 15|Exposure time:120 hpf|External Id:SAMEA13395712|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:53:11Z|INSDC status:public|Submitter Id:S TOXR1832:S 120hpf NK UHEI|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1832:S 120hpf NK UHEI|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1832:S 120hpf NK UHEI e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136288 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_120hpf_NK_UHEI.fastq.gz fastq 86664650.0 1733293.0 S TOXR1832:S 120hpf NK UHEI r 0:50 A:18560908;C:22622161;G:20286073;T:25183691;N:11817 50       18560908 22622161 20286073 25183691 11817 ERX8812300 ERS10998365 ERA10091710 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Larval Larval Cell Line Cell Line
10641 10641 ERR9269906 ERX8812303 ERS10998368 ERP136288 PRJEB51641 CS2 UHEI DART 96 120h raw data EUT053 S-TOXR1832 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13395715 University of Heidelberg Compound:DMSO|Concentration:0.1 %|ENA first public:2024 03 15|Exposure time:120 hpf|External Id:SAMEA13395715|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:53:12Z|INSDC status:public|Submitter Id:S TOXR1832:S 120hpf DMSO UHEI|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1832:S 120hpf DMSO UHEI|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1832:S 120hpf DMSO UHEI e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136288 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_120hpf_DMSO_UHEI.fastq.gz fastq 89616300.0 1792326.0 S TOXR1832:S 120hpf DMSO UHEI r 0:50 A:19116916;C:23344528;G:20924511;T:26218135;N:12210 50       19116916 23344528 20924511 26218135 12210 ERX8812303 ERS10998368 ERA10091710 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Larval Larval Cell Line Cell Line
10643 10643 ERR9269925 ERX8812322 ERS10998389 ERP136288 PRJEB51641 CS2 UHEI DART 96 120h raw data EUT053 S-TOXR1832 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13395734 University of Heidelberg Compound:Par1|Concentration:250.0 µM|ENA first public:2024 03 15|Exposure time:96 hpf|External Id:SAMEA13395734|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:53:12Z|INSDC status:public|Submitter Id:S TOXR1832:S 96hpf 250 Par1 UHEI|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1832:S 96hpf 250 Par1 UHEI|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1832:S 96hpf 250 Par1 UHEI e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136288 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_96hpf_250_Par1_UHEI.fastq.gz fastq 96150100.0 1923002.0 S TOXR1832:S 96hpf 250 Par1 UHEI r 0:50 A:20310305;C:25128686;G:22290025;T:28408131;N:12953 50       20310305 25128686 22290025 28408131 12953 ERX8812322 ERS10998389 ERA10091710 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Larval Larval Cell Line Cell Line
10645 10645 ERR9269912 ERX8812309 ERS10998375 ERP136288 PRJEB51641 CS2 UHEI DART 96 120h raw data EUT053 S-TOXR1832 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13395721 University of Heidelberg Compound:VPA|Concentration:100.0 µM|ENA first public:2024 03 15|Exposure time:120 hpf|External Id:SAMEA13395721|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:53:12Z|INSDC status:public|Submitter Id:S TOXR1832:S 120hpf VPA100 UHEI|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1832:S 120hpf VPA100 UHEI|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1832:S 120hpf VPA100 UHEI e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136288 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_120hpf_VPA100_UHEI.fastq.gz fastq 89459950.0 1789199.0 S TOXR1832:S 120hpf VPA100 UHEI r 0:50 A:18937857;C:23260429;G:20527346;T:26722200;N:12118 50       18937857 23260429 20527346 26722200 12118 ERX8812309 ERS10998375 ERA10091710 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Larval Larval Cell Line Cell Line
10648 10648 ERR9269921 ERX8812318 ERS10998384 ERP136288 PRJEB51641 CS2 UHEI DART 96 120h raw data EUT053 S-TOXR1832 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13395730 University of Heidelberg Compound:VPA|Concentration:2.0 µM|ENA first public:2024 03 15|Exposure time:120 hpf|External Id:SAMEA13395730|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:53:12Z|INSDC status:public|Submitter Id:S TOXR1832:S 120hpf VPA2 UHEI|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1832:S 120hpf VPA2 UHEI|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1832:S 120hpf VPA2 UHEI e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136288 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_120hpf_VPA2_UHEI.fastq.gz fastq 67771050.0 1355421.0 S TOXR1832:S 120hpf VPA2 UHEI r 0:50 A:14454221;C:17723386;G:15909234;T:19674975;N:9234 50       14454221 17723386 15909234 19674975 9234 ERX8812318 ERS10998384 ERA10091710 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Larval Larval Cell Line Cell Line
30692 30692 SRR29655998 SRX25159999 SRS21850513 SRP494129 PRJNA1085664 Human norovirus replicates in macrophages migrating to intestinal tissues and changes their transcriptional profile GSE261163 Transcriptome Analysis Human noroviruses HuNoVs are a major cause of diarrheal disease yet critical aspects of their biology including cellular tropism remain unclear. While research traditionally focused on the intestinal epithelium the hypothesis that HuNoV infects macrophages has been recurrently discussed and is investigated here by a zebrafish larval model. Our study reveals that HuNoV capsid proteins and double stranded RNA colocalize within intestinal macrophages of infected zebrafish larvae while the negative strand RNA intermediate was detected within FACS sorted macrophages. Flow cytometry confirms viral replication within these macrophages constituting 9% of HuNoV?s host cells. Single cell RNA sequencing reaffirms their role in viral replication as all three open reading frames were mapped to individual macrophages. Yet macrophages were not required for productive infection given the limited changes in viral loads upon their absence. Identifying macrophages as host cells prompts a reevaluation of their role in HuNoV pathogenesis offering new directions for understanding and controlling this infection. Overall design: Intestines of uninfected and HuNoV infected zebrafish larvae were dissected and subjected to the preparation of a single cell suspension that was analyzed using scRNAseq. Subsequently an additional sample was analyzed on FACS sorted macrophages of HuNoV infected zebrafish larvae.   pubmed:39584740   GC145382 HuNoV infected replicate 1 scRNAseq GSM8370069   tissue:Tg mpeg:mCherry|cell line:Tg mpeg:mCherry|cell type:120 hpf|infection:HuNoV infected|geo loc name:missing|collection date:missing GC145382 HuNoV infected replicate 1 scRNAseq Cell Ranger v3.0 10X Genomics Pleasanton California USA was used for single cell analysis to demultiplex raw base call files from Illumina sequencing and to align reads to both the zebrafish reference genome Ensembl GRCz11.106 and the HuNoV reference genome Genbank JX459908.1. Cell Ranger output matrices were analyzed using the Seurat R package version 3.1 in RStudio. Assembly: hg38 Supplementary files format and content: Count Matrix Cell Ranger Tg mpeg:mCherry   Intestinal tissues were manually dissected and subjected to dissociation using 1mg/mL collagenase 40 µg/mL proteinase K 0.25% trypsin for 40 min at 37°C. Cells were resuspended in 0.04% BSA and live cells were selected via Optiprep density gradient according to the manufacterer's protocol. The single cell suspensions were immediately subjected to the 10X Genomics Chromium Controller with Chromium Single Cell 3′ Library & Gel Bead Kit v3.1. Libraries were constructed according to 10X Genomics Chromium Single Cell 3’Library & Gel Bead Kit v3 protocol.   cell line:Tg mpeg:mCherry|cell type:120 hpf|infection:HuNoV infected GSM8370069 GSM8370069: GC145382 HuNoV infected replicate 1 scRNAseq; Danio rerio; RNA Seq GSM8370069 r1 GSM8370069 1 Intestinal tissues were manually dissected and subjected to dissociation using 1mg/mL collagenase 40 µg/mL proteinase K 0.25% trypsin for 40 min at 37°C. Cells were resuspended in 0.04% BSA and live cells were selected via Optiprep density gradient according to the manufacterer's protocol. The single cell suspensions were immediately subjected to the 10X Genomics Chromium Controller with Chromium Single Cell 3′ Library & Gel Bead Kit v3.1. Libraries were constructed according to 10X Genomics Chromium Single Cell three primeLibrary & Gel Bead Kit v3 protocol.   RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA PAIRED ILLUMINA Illumina NovaSeq 6000   SRP494129     GC145382_SI-GA-B1_S1_L001_R1_001.fastq.gz GC145382_SI-GA-B1_S1_L001_R2_001.fastq.gz fastq fastq 5781664526.0 48997157.0 GSM8370069 r1 0:28 1:90 A:1659071356;C:1268706433;G:1342749232;T:1510681009;N:456496 28 90     1659071356 1268706433 1342749232 1510681009 456496 SRX25159999 SRS21850513 SRA1913292 Laboratory of Virology and Chemotherapy, Department of Microbiology, Immunology, and Transplantation, KU Leuven, Rega Institute Laboratory of Virology and Chemotherapy, Department of Microbiology, Immunology, and Transplantation, KU Leuven, Rega Institute                       T B sc-like readlen illumina novaseq_era unknown cdna_unspecified unknown sc single_cell_droplet 10x   Belgium 2024-06-30 Larval Larval Cell Line Cell Line
30693 30693 SRR29655999 SRX25159999 SRS21850513 SRP494129 PRJNA1085664 Human norovirus replicates in macrophages migrating to intestinal tissues and changes their transcriptional profile GSE261163 Transcriptome Analysis Human noroviruses HuNoVs are a major cause of diarrheal disease yet critical aspects of their biology including cellular tropism remain unclear. While research traditionally focused on the intestinal epithelium the hypothesis that HuNoV infects macrophages has been recurrently discussed and is investigated here by a zebrafish larval model. Our study reveals that HuNoV capsid proteins and double stranded RNA colocalize within intestinal macrophages of infected zebrafish larvae while the negative strand RNA intermediate was detected within FACS sorted macrophages. Flow cytometry confirms viral replication within these macrophages constituting 9% of HuNoV?s host cells. Single cell RNA sequencing reaffirms their role in viral replication as all three open reading frames were mapped to individual macrophages. Yet macrophages were not required for productive infection given the limited changes in viral loads upon their absence. Identifying macrophages as host cells prompts a reevaluation of their role in HuNoV pathogenesis offering new directions for understanding and controlling this infection. Overall design: Intestines of uninfected and HuNoV infected zebrafish larvae were dissected and subjected to the preparation of a single cell suspension that was analyzed using scRNAseq. Subsequently an additional sample was analyzed on FACS sorted macrophages of HuNoV infected zebrafish larvae.   pubmed:39584740   GC145382 HuNoV infected replicate 1 scRNAseq GSM8370069   tissue:Tg mpeg:mCherry|cell line:Tg mpeg:mCherry|cell type:120 hpf|infection:HuNoV infected|geo loc name:missing|collection date:missing GC145382 HuNoV infected replicate 1 scRNAseq Cell Ranger v3.0 10X Genomics Pleasanton California USA was used for single cell analysis to demultiplex raw base call files from Illumina sequencing and to align reads to both the zebrafish reference genome Ensembl GRCz11.106 and the HuNoV reference genome Genbank JX459908.1. Cell Ranger output matrices were analyzed using the Seurat R package version 3.1 in RStudio. Assembly: hg38 Supplementary files format and content: Count Matrix Cell Ranger Tg mpeg:mCherry   Intestinal tissues were manually dissected and subjected to dissociation using 1mg/mL collagenase 40 µg/mL proteinase K 0.25% trypsin for 40 min at 37°C. Cells were resuspended in 0.04% BSA and live cells were selected via Optiprep density gradient according to the manufacterer's protocol. The single cell suspensions were immediately subjected to the 10X Genomics Chromium Controller with Chromium Single Cell 3′ Library & Gel Bead Kit v3.1. Libraries were constructed according to 10X Genomics Chromium Single Cell 3’Library & Gel Bead Kit v3 protocol.   cell line:Tg mpeg:mCherry|cell type:120 hpf|infection:HuNoV infected GSM8370069 GSM8370069: GC145382 HuNoV infected replicate 1 scRNAseq; Danio rerio; RNA Seq GSM8370069 r1 GSM8370069 1 Intestinal tissues were manually dissected and subjected to dissociation using 1mg/mL collagenase 40 µg/mL proteinase K 0.25% trypsin for 40 min at 37°C. Cells were resuspended in 0.04% BSA and live cells were selected via Optiprep density gradient according to the manufacterer's protocol. The single cell suspensions were immediately subjected to the 10X Genomics Chromium Controller with Chromium Single Cell 3′ Library & Gel Bead Kit v3.1. Libraries were constructed according to 10X Genomics Chromium Single Cell three primeLibrary & Gel Bead Kit v3 protocol.   RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA PAIRED ILLUMINA Illumina NovaSeq 6000   SRP494129     GC145382_SI-GA-B1_S1_L002_R1_001.fastq.gz GC145382_SI-GA-B1_S1_L002_R2_001.fastq.gz fastq fastq 6046508328.0 51241596.0 GSM8370069 r2 0:28 1:90 A:1731027394;C:1330440539;G:1408717533;T:1575870623;N:452239 28 90     1731027394 1330440539 1408717533 1575870623 452239 SRX25159999 SRS21850513 SRA1913292 Laboratory of Virology and Chemotherapy, Department of Microbiology, Immunology, and Transplantation, KU Leuven, Rega Institute Laboratory of Virology and Chemotherapy, Department of Microbiology, Immunology, and Transplantation, KU Leuven, Rega Institute                       T B sc-like readlen illumina novaseq_era unknown cdna_unspecified unknown sc single_cell_droplet 10x   Belgium 2024-06-30 Larval Larval Cell Line Cell Line
32865 32865 SRR29488925 SRX24999628 SRS21700588 SRP515253 PRJNA1126644 Elevated MG H1 in glo1 / aldh3a1 / zebrafish caused microvasculature alterations and glucose metabolism impairment via decreased proteasome activity GSE270442 Transcriptome Analysis Dicarbonyl stress is characterized by the abnormal accumulation of dicarbonyl reactive metabolites leading to increased modification of proteins DNA and lipids thereby contributing to cellular and tissue dysfunction in diabetes diabetic complications and other diseases. glo1 knockout zebrafish exhibited moderately increased MG levels yet this was inadequate to induce trunk vessels alterations due to elevated ALDH activity and mRNA expression levels which partially act as compensatory mechanism. Excess 4 HNE induced pancreas dysfunction in aldh3a1 knockout zebrafish larvae inhibiting insulin expression and thereby facilitating hyperglycemia and hyaloid vasculature alterations. To evaluate the combined function of Glo1 and Aldh3a1 in glucose homeostasis and diabetic microvasculature glo1 / aldh3a1 / zebrafish were generated using CRISPR/Cas9 technology. Multiple experiments are performed regarding vasculature alterations glucose homeostasis transcriptome and metabolomics in Tgfli1:EGFP zebrafish. glo1 / aldh3a1 / zebrafish larvae displayed angiogenic hyaloid vasculature caused by the elevated MG H1 and decreased proteasomal chymotrypsin like activity which could be rescued by the MG H1 scavenger L carnosine and proteasome activator Betulinic acid treatments. In adult glo1 / aldh3a1 / zebrafish impaired glucose metabolism angiogenic retina vasculature and thickened GBM were observed. Thus our data suggested that an important upstream factor contributing to these phenomena in the context of lacking glo1 and aldh3a1 is MG H1 possibly through reduced proteasome activity. Overall design: glo1 / aldh3a1 / zebrafish were generated using CRISPR/Cas9 technology. Multiple experiments are performed regarding vasculature alterations glucose homeostasis transcriptome and metabolomics in Tgfli1:EGFP zebrafish.       DKO5 GSM8343040   source name:larvae|tissue:larvae|cell line:Tgfli1:EGFP|cell type:larvae|genotype:DKO|geo loc name:missing|collection date:missing DKO5 Raw sequence data with adapter sequences or low quality sequences was filtered. Filtered reads were aligned to the Zebrafish reference genome GRCz11 Raw counts levels were quantified using FeatureCounts v2.0.3 Assembly: GRCz11 Supplementary files format and content: tab delimited text file includes raw counts for each sample. larvae   Total RNA was extracted using RNeasy Mini KitQIAGEN 3ug of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA seq were prepared using DNBSEQ Eukaryotic Strand specific mRNA library.   tissue:larvae|cell line:Tgfli1:EGFP|cell type:larvae|genotype:DKO GSM8343040 GSM8343040: DKO5; Danio rerio; RNA Seq GSM8343040 r1 GSM8343040 1 Total RNA was extracted using RNeasy Mini KitQIAGEN 3ug of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA seq were prepared using DNBSEQ Eukaryotic Strand specific mRNA library.   RNA-Seq TRANSCRIPTOMIC cDNA PAIRED DNBSEQ DNBSEQ-G400   SRP515253     DKO5_1.fq.gz DKO5_2.fq.gz fastq fastq 4813408600.0 24067043.0 GSM8343040 r1 0:100 1:100 A:1302917202;C:1105312585;G:1123058369;T:1282120444;N:0 100 100     1302917202 1105312585 1123058369 1282120444 0 SRX24999628 SRS21700588 SRA1904686 ECAS ECAS                       B B biological fallback assumption bgi bgi unknown cdna_unspecified unknown bulk unknown unknown   Unknown 2024-06-21 Larval Larval Cell Line Cell Line
32866 32866 SRR29488926 SRX24999627 SRS21700587 SRP515253 PRJNA1126644 Elevated MG H1 in glo1 / aldh3a1 / zebrafish caused microvasculature alterations and glucose metabolism impairment via decreased proteasome activity GSE270442 Transcriptome Analysis Dicarbonyl stress is characterized by the abnormal accumulation of dicarbonyl reactive metabolites leading to increased modification of proteins DNA and lipids thereby contributing to cellular and tissue dysfunction in diabetes diabetic complications and other diseases. glo1 knockout zebrafish exhibited moderately increased MG levels yet this was inadequate to induce trunk vessels alterations due to elevated ALDH activity and mRNA expression levels which partially act as compensatory mechanism. Excess 4 HNE induced pancreas dysfunction in aldh3a1 knockout zebrafish larvae inhibiting insulin expression and thereby facilitating hyperglycemia and hyaloid vasculature alterations. To evaluate the combined function of Glo1 and Aldh3a1 in glucose homeostasis and diabetic microvasculature glo1 / aldh3a1 / zebrafish were generated using CRISPR/Cas9 technology. Multiple experiments are performed regarding vasculature alterations glucose homeostasis transcriptome and metabolomics in Tgfli1:EGFP zebrafish. glo1 / aldh3a1 / zebrafish larvae displayed angiogenic hyaloid vasculature caused by the elevated MG H1 and decreased proteasomal chymotrypsin like activity which could be rescued by the MG H1 scavenger L carnosine and proteasome activator Betulinic acid treatments. In adult glo1 / aldh3a1 / zebrafish impaired glucose metabolism angiogenic retina vasculature and thickened GBM were observed. Thus our data suggested that an important upstream factor contributing to these phenomena in the context of lacking glo1 and aldh3a1 is MG H1 possibly through reduced proteasome activity. Overall design: glo1 / aldh3a1 / zebrafish were generated using CRISPR/Cas9 technology. Multiple experiments are performed regarding vasculature alterations glucose homeostasis transcriptome and metabolomics in Tgfli1:EGFP zebrafish.       DKO4 GSM8343039   source name:larvae|tissue:larvae|cell line:Tgfli1:EGFP|cell type:larvae|genotype:DKO|geo loc name:missing|collection date:missing DKO4 Raw sequence data with adapter sequences or low quality sequences was filtered. Filtered reads were aligned to the Zebrafish reference genome GRCz11 Raw counts levels were quantified using FeatureCounts v2.0.3 Assembly: GRCz11 Supplementary files format and content: tab delimited text file includes raw counts for each sample. larvae   Total RNA was extracted using RNeasy Mini KitQIAGEN 3ug of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA seq were prepared using DNBSEQ Eukaryotic Strand specific mRNA library.   tissue:larvae|cell line:Tgfli1:EGFP|cell type:larvae|genotype:DKO GSM8343039 GSM8343039: DKO4; Danio rerio; RNA Seq GSM8343039 r1 GSM8343039 1 Total RNA was extracted using RNeasy Mini KitQIAGEN 3ug of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA seq were prepared using DNBSEQ Eukaryotic Strand specific mRNA library.   RNA-Seq TRANSCRIPTOMIC cDNA PAIRED DNBSEQ DNBSEQ-G400   SRP515253     DKO4_1.fq.gz DKO4_2.fq.gz fastq fastq 4825860200.0 24129301.0 GSM8343039 r1 0:100 1:100 A:1303584214;C:1108617528;G:1131675283;T:1281983175;N:0 100 100     1303584214 1108617528 1131675283 1281983175 0 SRX24999627 SRS21700587 SRA1904686 ECAS ECAS                       B B biological fallback assumption bgi bgi unknown cdna_unspecified unknown bulk unknown unknown   Unknown 2024-06-21 Larval Larval Cell Line Cell Line
32867 32867 SRR29488927 SRX24999626 SRS21700586 SRP515253 PRJNA1126644 Elevated MG H1 in glo1 / aldh3a1 / zebrafish caused microvasculature alterations and glucose metabolism impairment via decreased proteasome activity GSE270442 Transcriptome Analysis Dicarbonyl stress is characterized by the abnormal accumulation of dicarbonyl reactive metabolites leading to increased modification of proteins DNA and lipids thereby contributing to cellular and tissue dysfunction in diabetes diabetic complications and other diseases. glo1 knockout zebrafish exhibited moderately increased MG levels yet this was inadequate to induce trunk vessels alterations due to elevated ALDH activity and mRNA expression levels which partially act as compensatory mechanism. Excess 4 HNE induced pancreas dysfunction in aldh3a1 knockout zebrafish larvae inhibiting insulin expression and thereby facilitating hyperglycemia and hyaloid vasculature alterations. To evaluate the combined function of Glo1 and Aldh3a1 in glucose homeostasis and diabetic microvasculature glo1 / aldh3a1 / zebrafish were generated using CRISPR/Cas9 technology. Multiple experiments are performed regarding vasculature alterations glucose homeostasis transcriptome and metabolomics in Tgfli1:EGFP zebrafish. glo1 / aldh3a1 / zebrafish larvae displayed angiogenic hyaloid vasculature caused by the elevated MG H1 and decreased proteasomal chymotrypsin like activity which could be rescued by the MG H1 scavenger L carnosine and proteasome activator Betulinic acid treatments. In adult glo1 / aldh3a1 / zebrafish impaired glucose metabolism angiogenic retina vasculature and thickened GBM were observed. Thus our data suggested that an important upstream factor contributing to these phenomena in the context of lacking glo1 and aldh3a1 is MG H1 possibly through reduced proteasome activity. Overall design: glo1 / aldh3a1 / zebrafish were generated using CRISPR/Cas9 technology. Multiple experiments are performed regarding vasculature alterations glucose homeostasis transcriptome and metabolomics in Tgfli1:EGFP zebrafish.       DKO3 GSM8343038   source name:larvae|tissue:larvae|cell line:Tgfli1:EGFP|cell type:larvae|genotype:DKO|geo loc name:missing|collection date:missing DKO3 Raw sequence data with adapter sequences or low quality sequences was filtered. Filtered reads were aligned to the Zebrafish reference genome GRCz11 Raw counts levels were quantified using FeatureCounts v2.0.3 Assembly: GRCz11 Supplementary files format and content: tab delimited text file includes raw counts for each sample. larvae   Total RNA was extracted using RNeasy Mini KitQIAGEN 3ug of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA seq were prepared using DNBSEQ Eukaryotic Strand specific mRNA library.   tissue:larvae|cell line:Tgfli1:EGFP|cell type:larvae|genotype:DKO GSM8343038 GSM8343038: DKO3; Danio rerio; RNA Seq GSM8343038 r1 GSM8343038 1 Total RNA was extracted using RNeasy Mini KitQIAGEN 3ug of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA seq were prepared using DNBSEQ Eukaryotic Strand specific mRNA library.   RNA-Seq TRANSCRIPTOMIC cDNA PAIRED DNBSEQ DNBSEQ-G400   SRP515253     DKO3_1.fq.gz DKO3_2.fq.gz fastq fastq 4806754400.0 24033772.0 GSM8343038 r1 0:100 1:100 A:1289902978;C:1113397086;G:1131727783;T:1271726553;N:0 100 100     1289902978 1113397086 1131727783 1271726553 0 SRX24999626 SRS21700586 SRA1904686 ECAS ECAS                       B B biological fallback assumption bgi bgi unknown cdna_unspecified unknown bulk unknown unknown   Unknown 2024-06-21 Larval Larval Cell Line Cell Line
32868 32868 SRR29488928 SRX24999625 SRS21700585 SRP515253 PRJNA1126644 Elevated MG H1 in glo1 / aldh3a1 / zebrafish caused microvasculature alterations and glucose metabolism impairment via decreased proteasome activity GSE270442 Transcriptome Analysis Dicarbonyl stress is characterized by the abnormal accumulation of dicarbonyl reactive metabolites leading to increased modification of proteins DNA and lipids thereby contributing to cellular and tissue dysfunction in diabetes diabetic complications and other diseases. glo1 knockout zebrafish exhibited moderately increased MG levels yet this was inadequate to induce trunk vessels alterations due to elevated ALDH activity and mRNA expression levels which partially act as compensatory mechanism. Excess 4 HNE induced pancreas dysfunction in aldh3a1 knockout zebrafish larvae inhibiting insulin expression and thereby facilitating hyperglycemia and hyaloid vasculature alterations. To evaluate the combined function of Glo1 and Aldh3a1 in glucose homeostasis and diabetic microvasculature glo1 / aldh3a1 / zebrafish were generated using CRISPR/Cas9 technology. Multiple experiments are performed regarding vasculature alterations glucose homeostasis transcriptome and metabolomics in Tgfli1:EGFP zebrafish. glo1 / aldh3a1 / zebrafish larvae displayed angiogenic hyaloid vasculature caused by the elevated MG H1 and decreased proteasomal chymotrypsin like activity which could be rescued by the MG H1 scavenger L carnosine and proteasome activator Betulinic acid treatments. In adult glo1 / aldh3a1 / zebrafish impaired glucose metabolism angiogenic retina vasculature and thickened GBM were observed. Thus our data suggested that an important upstream factor contributing to these phenomena in the context of lacking glo1 and aldh3a1 is MG H1 possibly through reduced proteasome activity. Overall design: glo1 / aldh3a1 / zebrafish were generated using CRISPR/Cas9 technology. Multiple experiments are performed regarding vasculature alterations glucose homeostasis transcriptome and metabolomics in Tgfli1:EGFP zebrafish.       DKO2 GSM8343037   source name:larvae|tissue:larvae|cell line:Tgfli1:EGFP|cell type:larvae|genotype:DKO|geo loc name:missing|collection date:missing DKO2 Raw sequence data with adapter sequences or low quality sequences was filtered. Filtered reads were aligned to the Zebrafish reference genome GRCz11 Raw counts levels were quantified using FeatureCounts v2.0.3 Assembly: GRCz11 Supplementary files format and content: tab delimited text file includes raw counts for each sample. larvae   Total RNA was extracted using RNeasy Mini KitQIAGEN 3ug of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA seq were prepared using DNBSEQ Eukaryotic Strand specific mRNA library.   tissue:larvae|cell line:Tgfli1:EGFP|cell type:larvae|genotype:DKO GSM8343037 GSM8343037: DKO2; Danio rerio; RNA Seq GSM8343037 r1 GSM8343037 1 Total RNA was extracted using RNeasy Mini KitQIAGEN 3ug of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA seq were prepared using DNBSEQ Eukaryotic Strand specific mRNA library.   RNA-Seq TRANSCRIPTOMIC cDNA PAIRED DNBSEQ DNBSEQ-G400   SRP515253     DKO2_1.fq.gz DKO2_2.fq.gz fastq fastq 4805757800.0 24028789.0 GSM8343037 r1 0:100 1:100 A:1299804990;C:1103101159;G:1124220106;T:1278631545;N:0 100 100     1299804990 1103101159 1124220106 1278631545 0 SRX24999625 SRS21700585 SRA1904686 ECAS ECAS                       B B biological fallback assumption bgi bgi unknown cdna_unspecified unknown bulk unknown unknown   Unknown 2024-06-21 Larval Larval Cell Line Cell Line
32869 32869 SRR29488929 SRX24999624 SRS21700584 SRP515253 PRJNA1126644 Elevated MG H1 in glo1 / aldh3a1 / zebrafish caused microvasculature alterations and glucose metabolism impairment via decreased proteasome activity GSE270442 Transcriptome Analysis Dicarbonyl stress is characterized by the abnormal accumulation of dicarbonyl reactive metabolites leading to increased modification of proteins DNA and lipids thereby contributing to cellular and tissue dysfunction in diabetes diabetic complications and other diseases. glo1 knockout zebrafish exhibited moderately increased MG levels yet this was inadequate to induce trunk vessels alterations due to elevated ALDH activity and mRNA expression levels which partially act as compensatory mechanism. Excess 4 HNE induced pancreas dysfunction in aldh3a1 knockout zebrafish larvae inhibiting insulin expression and thereby facilitating hyperglycemia and hyaloid vasculature alterations. To evaluate the combined function of Glo1 and Aldh3a1 in glucose homeostasis and diabetic microvasculature glo1 / aldh3a1 / zebrafish were generated using CRISPR/Cas9 technology. Multiple experiments are performed regarding vasculature alterations glucose homeostasis transcriptome and metabolomics in Tgfli1:EGFP zebrafish. glo1 / aldh3a1 / zebrafish larvae displayed angiogenic hyaloid vasculature caused by the elevated MG H1 and decreased proteasomal chymotrypsin like activity which could be rescued by the MG H1 scavenger L carnosine and proteasome activator Betulinic acid treatments. In adult glo1 / aldh3a1 / zebrafish impaired glucose metabolism angiogenic retina vasculature and thickened GBM were observed. Thus our data suggested that an important upstream factor contributing to these phenomena in the context of lacking glo1 and aldh3a1 is MG H1 possibly through reduced proteasome activity. Overall design: glo1 / aldh3a1 / zebrafish were generated using CRISPR/Cas9 technology. Multiple experiments are performed regarding vasculature alterations glucose homeostasis transcriptome and metabolomics in Tgfli1:EGFP zebrafish.       DKO1 GSM8343036   source name:larvae|tissue:larvae|cell line:Tgfli1:EGFP|cell type:larvae|genotype:DKO|geo loc name:missing|collection date:missing DKO1 Raw sequence data with adapter sequences or low quality sequences was filtered. Filtered reads were aligned to the Zebrafish reference genome GRCz11 Raw counts levels were quantified using FeatureCounts v2.0.3 Assembly: GRCz11 Supplementary files format and content: tab delimited text file includes raw counts for each sample. larvae   Total RNA was extracted using RNeasy Mini KitQIAGEN 3ug of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA seq were prepared using DNBSEQ Eukaryotic Strand specific mRNA library.   tissue:larvae|cell line:Tgfli1:EGFP|cell type:larvae|genotype:DKO GSM8343036 GSM8343036: DKO1; Danio rerio; RNA Seq GSM8343036 r1 GSM8343036 1 Total RNA was extracted using RNeasy Mini KitQIAGEN 3ug of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA seq were prepared using DNBSEQ Eukaryotic Strand specific mRNA library.   RNA-Seq TRANSCRIPTOMIC cDNA PAIRED DNBSEQ DNBSEQ-G400   SRP515253     DKO1_1.fq.gz DKO1_2.fq.gz fastq fastq 4819172400.0 24095862.0 GSM8343036 r1 0:100 1:100 A:1296017748;C:1112321357;G:1133115185;T:1277718110;N:0 100 100     1296017748 1112321357 1133115185 1277718110 0 SRX24999624 SRS21700584 SRA1904686 ECAS ECAS                       B B biological fallback assumption bgi bgi unknown cdna_unspecified unknown bulk unknown unknown   Unknown 2024-06-21 Larval Larval Cell Line Cell Line
32870 32870 SRR29488930 SRX24999623 SRS21700583 SRP515253 PRJNA1126644 Elevated MG H1 in glo1 / aldh3a1 / zebrafish caused microvasculature alterations and glucose metabolism impairment via decreased proteasome activity GSE270442 Transcriptome Analysis Dicarbonyl stress is characterized by the abnormal accumulation of dicarbonyl reactive metabolites leading to increased modification of proteins DNA and lipids thereby contributing to cellular and tissue dysfunction in diabetes diabetic complications and other diseases. glo1 knockout zebrafish exhibited moderately increased MG levels yet this was inadequate to induce trunk vessels alterations due to elevated ALDH activity and mRNA expression levels which partially act as compensatory mechanism. Excess 4 HNE induced pancreas dysfunction in aldh3a1 knockout zebrafish larvae inhibiting insulin expression and thereby facilitating hyperglycemia and hyaloid vasculature alterations. To evaluate the combined function of Glo1 and Aldh3a1 in glucose homeostasis and diabetic microvasculature glo1 / aldh3a1 / zebrafish were generated using CRISPR/Cas9 technology. Multiple experiments are performed regarding vasculature alterations glucose homeostasis transcriptome and metabolomics in Tgfli1:EGFP zebrafish. glo1 / aldh3a1 / zebrafish larvae displayed angiogenic hyaloid vasculature caused by the elevated MG H1 and decreased proteasomal chymotrypsin like activity which could be rescued by the MG H1 scavenger L carnosine and proteasome activator Betulinic acid treatments. In adult glo1 / aldh3a1 / zebrafish impaired glucose metabolism angiogenic retina vasculature and thickened GBM were observed. Thus our data suggested that an important upstream factor contributing to these phenomena in the context of lacking glo1 and aldh3a1 is MG H1 possibly through reduced proteasome activity. Overall design: glo1 / aldh3a1 / zebrafish were generated using CRISPR/Cas9 technology. Multiple experiments are performed regarding vasculature alterations glucose homeostasis transcriptome and metabolomics in Tgfli1:EGFP zebrafish.       GLO1KO5 GSM8343035   source name:larvae|tissue:larvae|cell line:Tgfli1:EGFP|cell type:larvae|genotype:GLO1KO|geo loc name:missing|collection date:missing GLO1KO5 Raw sequence data with adapter sequences or low quality sequences was filtered. Filtered reads were aligned to the Zebrafish reference genome GRCz11 Raw counts levels were quantified using FeatureCounts v2.0.3 Assembly: GRCz11 Supplementary files format and content: tab delimited text file includes raw counts for each sample. larvae   Total RNA was extracted using RNeasy Mini KitQIAGEN 3ug of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA seq were prepared using DNBSEQ Eukaryotic Strand specific mRNA library.   tissue:larvae|cell line:Tgfli1:EGFP|cell type:larvae|genotype:GLO1KO GSM8343035 GSM8343035: GLO1KO5; Danio rerio; RNA Seq GSM8343035 r1 GSM8343035 1 Total RNA was extracted using RNeasy Mini KitQIAGEN 3ug of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA seq were prepared using DNBSEQ Eukaryotic Strand specific mRNA library.   RNA-Seq TRANSCRIPTOMIC cDNA PAIRED DNBSEQ DNBSEQ-G400   SRP515253     GLO1KO5_1.fq.gz GLO1KO5_2.fq.gz fastq fastq 4813614800.0 24068074.0 GSM8343035 r1 0:100 1:100 A:1289774342;C:1116099072;G:1136080400;T:1271660986;N:0 100 100     1289774342 1116099072 1136080400 1271660986 0 SRX24999623 SRS21700583 SRA1904686 ECAS ECAS                       B B biological fallback assumption bgi bgi unknown cdna_unspecified unknown bulk unknown unknown   Unknown 2024-06-21 Larval Larval Cell Line Cell Line
32871 32871 SRR29488931 SRX24999622 SRS21700582 SRP515253 PRJNA1126644 Elevated MG H1 in glo1 / aldh3a1 / zebrafish caused microvasculature alterations and glucose metabolism impairment via decreased proteasome activity GSE270442 Transcriptome Analysis Dicarbonyl stress is characterized by the abnormal accumulation of dicarbonyl reactive metabolites leading to increased modification of proteins DNA and lipids thereby contributing to cellular and tissue dysfunction in diabetes diabetic complications and other diseases. glo1 knockout zebrafish exhibited moderately increased MG levels yet this was inadequate to induce trunk vessels alterations due to elevated ALDH activity and mRNA expression levels which partially act as compensatory mechanism. Excess 4 HNE induced pancreas dysfunction in aldh3a1 knockout zebrafish larvae inhibiting insulin expression and thereby facilitating hyperglycemia and hyaloid vasculature alterations. To evaluate the combined function of Glo1 and Aldh3a1 in glucose homeostasis and diabetic microvasculature glo1 / aldh3a1 / zebrafish were generated using CRISPR/Cas9 technology. Multiple experiments are performed regarding vasculature alterations glucose homeostasis transcriptome and metabolomics in Tgfli1:EGFP zebrafish. glo1 / aldh3a1 / zebrafish larvae displayed angiogenic hyaloid vasculature caused by the elevated MG H1 and decreased proteasomal chymotrypsin like activity which could be rescued by the MG H1 scavenger L carnosine and proteasome activator Betulinic acid treatments. In adult glo1 / aldh3a1 / zebrafish impaired glucose metabolism angiogenic retina vasculature and thickened GBM were observed. Thus our data suggested that an important upstream factor contributing to these phenomena in the context of lacking glo1 and aldh3a1 is MG H1 possibly through reduced proteasome activity. Overall design: glo1 / aldh3a1 / zebrafish were generated using CRISPR/Cas9 technology. Multiple experiments are performed regarding vasculature alterations glucose homeostasis transcriptome and metabolomics in Tgfli1:EGFP zebrafish.       GLO1KO4 GSM8343034   source name:larvae|tissue:larvae|cell line:Tgfli1:EGFP|cell type:larvae|genotype:GLO1KO|geo loc name:missing|collection date:missing GLO1KO4 Raw sequence data with adapter sequences or low quality sequences was filtered. Filtered reads were aligned to the Zebrafish reference genome GRCz11 Raw counts levels were quantified using FeatureCounts v2.0.3 Assembly: GRCz11 Supplementary files format and content: tab delimited text file includes raw counts for each sample. larvae   Total RNA was extracted using RNeasy Mini KitQIAGEN 3ug of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA seq were prepared using DNBSEQ Eukaryotic Strand specific mRNA library.   tissue:larvae|cell line:Tgfli1:EGFP|cell type:larvae|genotype:GLO1KO GSM8343034 GSM8343034: GLO1KO4; Danio rerio; RNA Seq GSM8343034 r1 GSM8343034 1 Total RNA was extracted using RNeasy Mini KitQIAGEN 3ug of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA seq were prepared using DNBSEQ Eukaryotic Strand specific mRNA library.   RNA-Seq TRANSCRIPTOMIC cDNA PAIRED DNBSEQ DNBSEQ-G400   SRP515253     GLO1KO4_1.fq.gz GLO1KO4_2.fq.gz fastq fastq 4805954200.0 24029771.0 GSM8343034 r1 0:100 1:100 A:1288997011;C:1113616764;G:1132409574;T:1270930851;N:0 100 100     1288997011 1113616764 1132409574 1270930851 0 SRX24999622 SRS21700582 SRA1904686 ECAS ECAS                       B B biological fallback assumption bgi bgi unknown cdna_unspecified unknown bulk unknown unknown   Unknown 2024-06-21 Larval Larval Cell Line Cell Line
32872 32872 SRR29488932 SRX24999621 SRS21700581 SRP515253 PRJNA1126644 Elevated MG H1 in glo1 / aldh3a1 / zebrafish caused microvasculature alterations and glucose metabolism impairment via decreased proteasome activity GSE270442 Transcriptome Analysis Dicarbonyl stress is characterized by the abnormal accumulation of dicarbonyl reactive metabolites leading to increased modification of proteins DNA and lipids thereby contributing to cellular and tissue dysfunction in diabetes diabetic complications and other diseases. glo1 knockout zebrafish exhibited moderately increased MG levels yet this was inadequate to induce trunk vessels alterations due to elevated ALDH activity and mRNA expression levels which partially act as compensatory mechanism. Excess 4 HNE induced pancreas dysfunction in aldh3a1 knockout zebrafish larvae inhibiting insulin expression and thereby facilitating hyperglycemia and hyaloid vasculature alterations. To evaluate the combined function of Glo1 and Aldh3a1 in glucose homeostasis and diabetic microvasculature glo1 / aldh3a1 / zebrafish were generated using CRISPR/Cas9 technology. Multiple experiments are performed regarding vasculature alterations glucose homeostasis transcriptome and metabolomics in Tgfli1:EGFP zebrafish. glo1 / aldh3a1 / zebrafish larvae displayed angiogenic hyaloid vasculature caused by the elevated MG H1 and decreased proteasomal chymotrypsin like activity which could be rescued by the MG H1 scavenger L carnosine and proteasome activator Betulinic acid treatments. In adult glo1 / aldh3a1 / zebrafish impaired glucose metabolism angiogenic retina vasculature and thickened GBM were observed. Thus our data suggested that an important upstream factor contributing to these phenomena in the context of lacking glo1 and aldh3a1 is MG H1 possibly through reduced proteasome activity. Overall design: glo1 / aldh3a1 / zebrafish were generated using CRISPR/Cas9 technology. Multiple experiments are performed regarding vasculature alterations glucose homeostasis transcriptome and metabolomics in Tgfli1:EGFP zebrafish.       GLO1KO3 GSM8343033   source name:larvae|tissue:larvae|cell line:Tgfli1:EGFP|cell type:larvae|genotype:GLO1KO|geo loc name:missing|collection date:missing GLO1KO3 Raw sequence data with adapter sequences or low quality sequences was filtered. Filtered reads were aligned to the Zebrafish reference genome GRCz11 Raw counts levels were quantified using FeatureCounts v2.0.3 Assembly: GRCz11 Supplementary files format and content: tab delimited text file includes raw counts for each sample. larvae   Total RNA was extracted using RNeasy Mini KitQIAGEN 3ug of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA seq were prepared using DNBSEQ Eukaryotic Strand specific mRNA library.   tissue:larvae|cell line:Tgfli1:EGFP|cell type:larvae|genotype:GLO1KO GSM8343033 GSM8343033: GLO1KO3; Danio rerio; RNA Seq GSM8343033 r1 GSM8343033 1 Total RNA was extracted using RNeasy Mini KitQIAGEN 3ug of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA seq were prepared using DNBSEQ Eukaryotic Strand specific mRNA library.   RNA-Seq TRANSCRIPTOMIC cDNA PAIRED DNBSEQ DNBSEQ-G400   SRP515253     GLO1KO3_1.fq.gz GLO1KO3_2.fq.gz fastq fastq 4817965600.0 24089828.0 GSM8343033 r1 0:100 1:100 A:1290593290;C:1118086028;G:1134064137;T:1275222145;N:0 100 100     1290593290 1118086028 1134064137 1275222145 0 SRX24999621 SRS21700581 SRA1904686 ECAS ECAS                       B B biological fallback assumption bgi bgi unknown cdna_unspecified unknown bulk unknown unknown   Unknown 2024-06-21 Larval Larval Cell Line Cell Line
32873 32873 SRR29488933 SRX24999620 SRS21700580 SRP515253 PRJNA1126644 Elevated MG H1 in glo1 / aldh3a1 / zebrafish caused microvasculature alterations and glucose metabolism impairment via decreased proteasome activity GSE270442 Transcriptome Analysis Dicarbonyl stress is characterized by the abnormal accumulation of dicarbonyl reactive metabolites leading to increased modification of proteins DNA and lipids thereby contributing to cellular and tissue dysfunction in diabetes diabetic complications and other diseases. glo1 knockout zebrafish exhibited moderately increased MG levels yet this was inadequate to induce trunk vessels alterations due to elevated ALDH activity and mRNA expression levels which partially act as compensatory mechanism. Excess 4 HNE induced pancreas dysfunction in aldh3a1 knockout zebrafish larvae inhibiting insulin expression and thereby facilitating hyperglycemia and hyaloid vasculature alterations. To evaluate the combined function of Glo1 and Aldh3a1 in glucose homeostasis and diabetic microvasculature glo1 / aldh3a1 / zebrafish were generated using CRISPR/Cas9 technology. Multiple experiments are performed regarding vasculature alterations glucose homeostasis transcriptome and metabolomics in Tgfli1:EGFP zebrafish. glo1 / aldh3a1 / zebrafish larvae displayed angiogenic hyaloid vasculature caused by the elevated MG H1 and decreased proteasomal chymotrypsin like activity which could be rescued by the MG H1 scavenger L carnosine and proteasome activator Betulinic acid treatments. In adult glo1 / aldh3a1 / zebrafish impaired glucose metabolism angiogenic retina vasculature and thickened GBM were observed. Thus our data suggested that an important upstream factor contributing to these phenomena in the context of lacking glo1 and aldh3a1 is MG H1 possibly through reduced proteasome activity. Overall design: glo1 / aldh3a1 / zebrafish were generated using CRISPR/Cas9 technology. Multiple experiments are performed regarding vasculature alterations glucose homeostasis transcriptome and metabolomics in Tgfli1:EGFP zebrafish.       GLO1KO2 GSM8343032   source name:larvae|tissue:larvae|cell line:Tgfli1:EGFP|cell type:larvae|genotype:GLO1KO|geo loc name:missing|collection date:missing GLO1KO2 Raw sequence data with adapter sequences or low quality sequences was filtered. Filtered reads were aligned to the Zebrafish reference genome GRCz11 Raw counts levels were quantified using FeatureCounts v2.0.3 Assembly: GRCz11 Supplementary files format and content: tab delimited text file includes raw counts for each sample. larvae   Total RNA was extracted using RNeasy Mini KitQIAGEN 3ug of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA seq were prepared using DNBSEQ Eukaryotic Strand specific mRNA library.   tissue:larvae|cell line:Tgfli1:EGFP|cell type:larvae|genotype:GLO1KO GSM8343032 GSM8343032: GLO1KO2; Danio rerio; RNA Seq GSM8343032 r1 GSM8343032 1 Total RNA was extracted using RNeasy Mini KitQIAGEN 3ug of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA seq were prepared using DNBSEQ Eukaryotic Strand specific mRNA library.   RNA-Seq TRANSCRIPTOMIC cDNA PAIRED DNBSEQ DNBSEQ-G400   SRP515253     GLO1KO2_1.fq.gz GLO1KO2_2.fq.gz fastq fastq 4805999000.0 24029995.0 GSM8343032 r1 0:100 1:100 A:1293609489;C:1109265811;G:1128786421;T:1274337279;N:0 100 100     1293609489 1109265811 1128786421 1274337279 0 SRX24999620 SRS21700580 SRA1904686 ECAS ECAS                       B B biological fallback assumption bgi bgi unknown cdna_unspecified unknown bulk unknown unknown   Unknown 2024-06-21 Larval Larval Cell Line Cell Line
32874 32874 SRR29488934 SRX24999619 SRS21700579 SRP515253 PRJNA1126644 Elevated MG H1 in glo1 / aldh3a1 / zebrafish caused microvasculature alterations and glucose metabolism impairment via decreased proteasome activity GSE270442 Transcriptome Analysis Dicarbonyl stress is characterized by the abnormal accumulation of dicarbonyl reactive metabolites leading to increased modification of proteins DNA and lipids thereby contributing to cellular and tissue dysfunction in diabetes diabetic complications and other diseases. glo1 knockout zebrafish exhibited moderately increased MG levels yet this was inadequate to induce trunk vessels alterations due to elevated ALDH activity and mRNA expression levels which partially act as compensatory mechanism. Excess 4 HNE induced pancreas dysfunction in aldh3a1 knockout zebrafish larvae inhibiting insulin expression and thereby facilitating hyperglycemia and hyaloid vasculature alterations. To evaluate the combined function of Glo1 and Aldh3a1 in glucose homeostasis and diabetic microvasculature glo1 / aldh3a1 / zebrafish were generated using CRISPR/Cas9 technology. Multiple experiments are performed regarding vasculature alterations glucose homeostasis transcriptome and metabolomics in Tgfli1:EGFP zebrafish. glo1 / aldh3a1 / zebrafish larvae displayed angiogenic hyaloid vasculature caused by the elevated MG H1 and decreased proteasomal chymotrypsin like activity which could be rescued by the MG H1 scavenger L carnosine and proteasome activator Betulinic acid treatments. In adult glo1 / aldh3a1 / zebrafish impaired glucose metabolism angiogenic retina vasculature and thickened GBM were observed. Thus our data suggested that an important upstream factor contributing to these phenomena in the context of lacking glo1 and aldh3a1 is MG H1 possibly through reduced proteasome activity. Overall design: glo1 / aldh3a1 / zebrafish were generated using CRISPR/Cas9 technology. Multiple experiments are performed regarding vasculature alterations glucose homeostasis transcriptome and metabolomics in Tgfli1:EGFP zebrafish.       GLO1KO1 GSM8343031   source name:larvae|tissue:larvae|cell line:Tgfli1:EGFP|cell type:larvae|genotype:GLO1KO|geo loc name:missing|collection date:missing GLO1KO1 Raw sequence data with adapter sequences or low quality sequences was filtered. Filtered reads were aligned to the Zebrafish reference genome GRCz11 Raw counts levels were quantified using FeatureCounts v2.0.3 Assembly: GRCz11 Supplementary files format and content: tab delimited text file includes raw counts for each sample. larvae   Total RNA was extracted using RNeasy Mini KitQIAGEN 3ug of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA seq were prepared using DNBSEQ Eukaryotic Strand specific mRNA library.   tissue:larvae|cell line:Tgfli1:EGFP|cell type:larvae|genotype:GLO1KO GSM8343031 GSM8343031: GLO1KO1; Danio rerio; RNA Seq GSM8343031 r1 GSM8343031 1 Total RNA was extracted using RNeasy Mini KitQIAGEN 3ug of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA seq were prepared using DNBSEQ Eukaryotic Strand specific mRNA library.   RNA-Seq TRANSCRIPTOMIC cDNA PAIRED DNBSEQ DNBSEQ-G400   SRP515253     GLO1KO1_1.fq.gz GLO1KO1_2.fq.gz fastq fastq 4806768400.0 24033842.0 GSM8343031 r1 0:100 1:100 A:1286467027;C:1115478546;G:1135313631;T:1269509196;N:0 100 100     1286467027 1115478546 1135313631 1269509196 0 SRX24999619 SRS21700579 SRA1904686 ECAS ECAS                       B B biological fallback assumption bgi bgi unknown cdna_unspecified unknown bulk unknown unknown   Unknown 2024-06-21 Larval Larval Cell Line Cell Line
32875 32875 SRR29488935 SRX24999618 SRS21700578 SRP515253 PRJNA1126644 Elevated MG H1 in glo1 / aldh3a1 / zebrafish caused microvasculature alterations and glucose metabolism impairment via decreased proteasome activity GSE270442 Transcriptome Analysis Dicarbonyl stress is characterized by the abnormal accumulation of dicarbonyl reactive metabolites leading to increased modification of proteins DNA and lipids thereby contributing to cellular and tissue dysfunction in diabetes diabetic complications and other diseases. glo1 knockout zebrafish exhibited moderately increased MG levels yet this was inadequate to induce trunk vessels alterations due to elevated ALDH activity and mRNA expression levels which partially act as compensatory mechanism. Excess 4 HNE induced pancreas dysfunction in aldh3a1 knockout zebrafish larvae inhibiting insulin expression and thereby facilitating hyperglycemia and hyaloid vasculature alterations. To evaluate the combined function of Glo1 and Aldh3a1 in glucose homeostasis and diabetic microvasculature glo1 / aldh3a1 / zebrafish were generated using CRISPR/Cas9 technology. Multiple experiments are performed regarding vasculature alterations glucose homeostasis transcriptome and metabolomics in Tgfli1:EGFP zebrafish. glo1 / aldh3a1 / zebrafish larvae displayed angiogenic hyaloid vasculature caused by the elevated MG H1 and decreased proteasomal chymotrypsin like activity which could be rescued by the MG H1 scavenger L carnosine and proteasome activator Betulinic acid treatments. In adult glo1 / aldh3a1 / zebrafish impaired glucose metabolism angiogenic retina vasculature and thickened GBM were observed. Thus our data suggested that an important upstream factor contributing to these phenomena in the context of lacking glo1 and aldh3a1 is MG H1 possibly through reduced proteasome activity. Overall design: glo1 / aldh3a1 / zebrafish were generated using CRISPR/Cas9 technology. Multiple experiments are performed regarding vasculature alterations glucose homeostasis transcriptome and metabolomics in Tgfli1:EGFP zebrafish.       ALDH3A1KO5 GSM8343030   source name:larvae|tissue:larvae|cell line:Tgfli1:EGFP|cell type:larvae|genotype:ALDH3A1KO|geo loc name:missing|collection date:missing ALDH3A1KO5 Raw sequence data with adapter sequences or low quality sequences was filtered. Filtered reads were aligned to the Zebrafish reference genome GRCz11 Raw counts levels were quantified using FeatureCounts v2.0.3 Assembly: GRCz11 Supplementary files format and content: tab delimited text file includes raw counts for each sample. larvae   Total RNA was extracted using RNeasy Mini KitQIAGEN 3ug of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA seq were prepared using DNBSEQ Eukaryotic Strand specific mRNA library.   tissue:larvae|cell line:Tgfli1:EGFP|cell type:larvae|genotype:ALDH3A1KO GSM8343030 GSM8343030: ALDH3A1KO5; Danio rerio; RNA Seq GSM8343030 r1 GSM8343030 1 Total RNA was extracted using RNeasy Mini KitQIAGEN 3ug of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA seq were prepared using DNBSEQ Eukaryotic Strand specific mRNA library.   RNA-Seq TRANSCRIPTOMIC cDNA PAIRED DNBSEQ DNBSEQ-G400   SRP515253     ALDH3A1KO5_1.fq.gz ALDH3A1KO5_2.fq.gz fastq fastq 4811331000.0 24056655.0 GSM8343030 r1 0:100 1:100 A:1314568654;C:1091419677;G:1111494171;T:1293848498;N:0 100 100     1314568654 1091419677 1111494171 1293848498 0 SRX24999618 SRS21700578 SRA1904686 ECAS ECAS                       B B biological fallback assumption bgi bgi unknown cdna_unspecified unknown bulk unknown unknown   Unknown 2024-06-21 Larval Larval Cell Line Cell Line
32876 32876 SRR29488936 SRX24999617 SRS21700577 SRP515253 PRJNA1126644 Elevated MG H1 in glo1 / aldh3a1 / zebrafish caused microvasculature alterations and glucose metabolism impairment via decreased proteasome activity GSE270442 Transcriptome Analysis Dicarbonyl stress is characterized by the abnormal accumulation of dicarbonyl reactive metabolites leading to increased modification of proteins DNA and lipids thereby contributing to cellular and tissue dysfunction in diabetes diabetic complications and other diseases. glo1 knockout zebrafish exhibited moderately increased MG levels yet this was inadequate to induce trunk vessels alterations due to elevated ALDH activity and mRNA expression levels which partially act as compensatory mechanism. Excess 4 HNE induced pancreas dysfunction in aldh3a1 knockout zebrafish larvae inhibiting insulin expression and thereby facilitating hyperglycemia and hyaloid vasculature alterations. To evaluate the combined function of Glo1 and Aldh3a1 in glucose homeostasis and diabetic microvasculature glo1 / aldh3a1 / zebrafish were generated using CRISPR/Cas9 technology. Multiple experiments are performed regarding vasculature alterations glucose homeostasis transcriptome and metabolomics in Tgfli1:EGFP zebrafish. glo1 / aldh3a1 / zebrafish larvae displayed angiogenic hyaloid vasculature caused by the elevated MG H1 and decreased proteasomal chymotrypsin like activity which could be rescued by the MG H1 scavenger L carnosine and proteasome activator Betulinic acid treatments. In adult glo1 / aldh3a1 / zebrafish impaired glucose metabolism angiogenic retina vasculature and thickened GBM were observed. Thus our data suggested that an important upstream factor contributing to these phenomena in the context of lacking glo1 and aldh3a1 is MG H1 possibly through reduced proteasome activity. Overall design: glo1 / aldh3a1 / zebrafish were generated using CRISPR/Cas9 technology. Multiple experiments are performed regarding vasculature alterations glucose homeostasis transcriptome and metabolomics in Tgfli1:EGFP zebrafish.       ALDH3A1KO4 GSM8343029   source name:larvae|tissue:larvae|cell line:Tgfli1:EGFP|cell type:larvae|genotype:ALDH3A1KO|geo loc name:missing|collection date:missing ALDH3A1KO4 Raw sequence data with adapter sequences or low quality sequences was filtered. Filtered reads were aligned to the Zebrafish reference genome GRCz11 Raw counts levels were quantified using FeatureCounts v2.0.3 Assembly: GRCz11 Supplementary files format and content: tab delimited text file includes raw counts for each sample. larvae   Total RNA was extracted using RNeasy Mini KitQIAGEN 3ug of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA seq were prepared using DNBSEQ Eukaryotic Strand specific mRNA library.   tissue:larvae|cell line:Tgfli1:EGFP|cell type:larvae|genotype:ALDH3A1KO GSM8343029 GSM8343029: ALDH3A1KO4; Danio rerio; RNA Seq GSM8343029 r1 GSM8343029 1 Total RNA was extracted using RNeasy Mini KitQIAGEN 3ug of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA seq were prepared using DNBSEQ Eukaryotic Strand specific mRNA library.   RNA-Seq TRANSCRIPTOMIC cDNA PAIRED DNBSEQ DNBSEQ-G400   SRP515253     ALDH3A1KO4_1.fq.gz ALDH3A1KO4_2.fq.gz fastq fastq 4816993800.0 24084969.0 GSM8343029 r1 0:100 1:100 A:1320840277;C:1087361823;G:1108886472;T:1299905228;N:0 100 100     1320840277 1087361823 1108886472 1299905228 0 SRX24999617 SRS21700577 SRA1904686 ECAS ECAS                       B B biological fallback assumption bgi bgi unknown cdna_unspecified unknown bulk unknown unknown   Unknown 2024-06-21 Larval Larval Cell Line Cell Line
32877 32877 SRR29488937 SRX24999616 SRS21700576 SRP515253 PRJNA1126644 Elevated MG H1 in glo1 / aldh3a1 / zebrafish caused microvasculature alterations and glucose metabolism impairment via decreased proteasome activity GSE270442 Transcriptome Analysis Dicarbonyl stress is characterized by the abnormal accumulation of dicarbonyl reactive metabolites leading to increased modification of proteins DNA and lipids thereby contributing to cellular and tissue dysfunction in diabetes diabetic complications and other diseases. glo1 knockout zebrafish exhibited moderately increased MG levels yet this was inadequate to induce trunk vessels alterations due to elevated ALDH activity and mRNA expression levels which partially act as compensatory mechanism. Excess 4 HNE induced pancreas dysfunction in aldh3a1 knockout zebrafish larvae inhibiting insulin expression and thereby facilitating hyperglycemia and hyaloid vasculature alterations. To evaluate the combined function of Glo1 and Aldh3a1 in glucose homeostasis and diabetic microvasculature glo1 / aldh3a1 / zebrafish were generated using CRISPR/Cas9 technology. Multiple experiments are performed regarding vasculature alterations glucose homeostasis transcriptome and metabolomics in Tgfli1:EGFP zebrafish. glo1 / aldh3a1 / zebrafish larvae displayed angiogenic hyaloid vasculature caused by the elevated MG H1 and decreased proteasomal chymotrypsin like activity which could be rescued by the MG H1 scavenger L carnosine and proteasome activator Betulinic acid treatments. In adult glo1 / aldh3a1 / zebrafish impaired glucose metabolism angiogenic retina vasculature and thickened GBM were observed. Thus our data suggested that an important upstream factor contributing to these phenomena in the context of lacking glo1 and aldh3a1 is MG H1 possibly through reduced proteasome activity. Overall design: glo1 / aldh3a1 / zebrafish were generated using CRISPR/Cas9 technology. Multiple experiments are performed regarding vasculature alterations glucose homeostasis transcriptome and metabolomics in Tgfli1:EGFP zebrafish.       ALDH3A1KO3 GSM8343028   source name:larvae|tissue:larvae|cell line:Tgfli1:EGFP|cell type:larvae|genotype:ALDH3A1KO|geo loc name:missing|collection date:missing ALDH3A1KO3 Raw sequence data with adapter sequences or low quality sequences was filtered. Filtered reads were aligned to the Zebrafish reference genome GRCz11 Raw counts levels were quantified using FeatureCounts v2.0.3 Assembly: GRCz11 Supplementary files format and content: tab delimited text file includes raw counts for each sample. larvae   Total RNA was extracted using RNeasy Mini KitQIAGEN 3ug of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA seq were prepared using DNBSEQ Eukaryotic Strand specific mRNA library.   tissue:larvae|cell line:Tgfli1:EGFP|cell type:larvae|genotype:ALDH3A1KO GSM8343028 GSM8343028: ALDH3A1KO3; Danio rerio; RNA Seq GSM8343028 r1 GSM8343028 1 Total RNA was extracted using RNeasy Mini KitQIAGEN 3ug of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA seq were prepared using DNBSEQ Eukaryotic Strand specific mRNA library.   RNA-Seq TRANSCRIPTOMIC cDNA PAIRED DNBSEQ DNBSEQ-G400   SRP515253     ALDH3A1KO3_1.fq.gz ALDH3A1KO3_2.fq.gz fastq fastq 4828261200.0 24141306.0 GSM8343028 r1 0:100 1:100 A:1325980327;C:1088988247;G:1107987702;T:1305304924;N:0 100 100     1325980327 1088988247 1107987702 1305304924 0 SRX24999616 SRS21700576 SRA1904686 ECAS ECAS                       B B biological fallback assumption bgi bgi unknown cdna_unspecified unknown bulk unknown unknown   Unknown 2024-06-21 Larval Larval Cell Line Cell Line
32878 32878 SRR29488938 SRX24999615 SRS21700575 SRP515253 PRJNA1126644 Elevated MG H1 in glo1 / aldh3a1 / zebrafish caused microvasculature alterations and glucose metabolism impairment via decreased proteasome activity GSE270442 Transcriptome Analysis Dicarbonyl stress is characterized by the abnormal accumulation of dicarbonyl reactive metabolites leading to increased modification of proteins DNA and lipids thereby contributing to cellular and tissue dysfunction in diabetes diabetic complications and other diseases. glo1 knockout zebrafish exhibited moderately increased MG levels yet this was inadequate to induce trunk vessels alterations due to elevated ALDH activity and mRNA expression levels which partially act as compensatory mechanism. Excess 4 HNE induced pancreas dysfunction in aldh3a1 knockout zebrafish larvae inhibiting insulin expression and thereby facilitating hyperglycemia and hyaloid vasculature alterations. To evaluate the combined function of Glo1 and Aldh3a1 in glucose homeostasis and diabetic microvasculature glo1 / aldh3a1 / zebrafish were generated using CRISPR/Cas9 technology. Multiple experiments are performed regarding vasculature alterations glucose homeostasis transcriptome and metabolomics in Tgfli1:EGFP zebrafish. glo1 / aldh3a1 / zebrafish larvae displayed angiogenic hyaloid vasculature caused by the elevated MG H1 and decreased proteasomal chymotrypsin like activity which could be rescued by the MG H1 scavenger L carnosine and proteasome activator Betulinic acid treatments. In adult glo1 / aldh3a1 / zebrafish impaired glucose metabolism angiogenic retina vasculature and thickened GBM were observed. Thus our data suggested that an important upstream factor contributing to these phenomena in the context of lacking glo1 and aldh3a1 is MG H1 possibly through reduced proteasome activity. Overall design: glo1 / aldh3a1 / zebrafish were generated using CRISPR/Cas9 technology. Multiple experiments are performed regarding vasculature alterations glucose homeostasis transcriptome and metabolomics in Tgfli1:EGFP zebrafish.       ALDH3A1KO2 GSM8343027   source name:larvae|tissue:larvae|cell line:Tgfli1:EGFP|cell type:larvae|genotype:ALDH3A1KO|geo loc name:missing|collection date:missing ALDH3A1KO2 Raw sequence data with adapter sequences or low quality sequences was filtered. Filtered reads were aligned to the Zebrafish reference genome GRCz11 Raw counts levels were quantified using FeatureCounts v2.0.3 Assembly: GRCz11 Supplementary files format and content: tab delimited text file includes raw counts for each sample. larvae   Total RNA was extracted using RNeasy Mini KitQIAGEN 3ug of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA seq were prepared using DNBSEQ Eukaryotic Strand specific mRNA library.   tissue:larvae|cell line:Tgfli1:EGFP|cell type:larvae|genotype:ALDH3A1KO GSM8343027 GSM8343027: ALDH3A1KO2; Danio rerio; RNA Seq GSM8343027 r1 GSM8343027 1 Total RNA was extracted using RNeasy Mini KitQIAGEN 3ug of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA seq were prepared using DNBSEQ Eukaryotic Strand specific mRNA library.   RNA-Seq TRANSCRIPTOMIC cDNA PAIRED DNBSEQ DNBSEQ-G400   SRP515253     ALDH3A1KO2_1.fq.gz ALDH3A1KO2_2.fq.gz fastq fastq 4806790400.0 24033952.0 GSM8343027 r1 0:100 1:100 A:1296924744;C:1110667292;G:1117161162;T:1282037202;N:0 100 100     1296924744 1110667292 1117161162 1282037202 0 SRX24999615 SRS21700575 SRA1904686 ECAS ECAS                       B B biological fallback assumption bgi bgi unknown cdna_unspecified unknown bulk unknown unknown   Unknown 2024-06-21 Larval Larval Cell Line Cell Line
32879 32879 SRR29488939 SRX24999614 SRS21700574 SRP515253 PRJNA1126644 Elevated MG H1 in glo1 / aldh3a1 / zebrafish caused microvasculature alterations and glucose metabolism impairment via decreased proteasome activity GSE270442 Transcriptome Analysis Dicarbonyl stress is characterized by the abnormal accumulation of dicarbonyl reactive metabolites leading to increased modification of proteins DNA and lipids thereby contributing to cellular and tissue dysfunction in diabetes diabetic complications and other diseases. glo1 knockout zebrafish exhibited moderately increased MG levels yet this was inadequate to induce trunk vessels alterations due to elevated ALDH activity and mRNA expression levels which partially act as compensatory mechanism. Excess 4 HNE induced pancreas dysfunction in aldh3a1 knockout zebrafish larvae inhibiting insulin expression and thereby facilitating hyperglycemia and hyaloid vasculature alterations. To evaluate the combined function of Glo1 and Aldh3a1 in glucose homeostasis and diabetic microvasculature glo1 / aldh3a1 / zebrafish were generated using CRISPR/Cas9 technology. Multiple experiments are performed regarding vasculature alterations glucose homeostasis transcriptome and metabolomics in Tgfli1:EGFP zebrafish. glo1 / aldh3a1 / zebrafish larvae displayed angiogenic hyaloid vasculature caused by the elevated MG H1 and decreased proteasomal chymotrypsin like activity which could be rescued by the MG H1 scavenger L carnosine and proteasome activator Betulinic acid treatments. In adult glo1 / aldh3a1 / zebrafish impaired glucose metabolism angiogenic retina vasculature and thickened GBM were observed. Thus our data suggested that an important upstream factor contributing to these phenomena in the context of lacking glo1 and aldh3a1 is MG H1 possibly through reduced proteasome activity. Overall design: glo1 / aldh3a1 / zebrafish were generated using CRISPR/Cas9 technology. Multiple experiments are performed regarding vasculature alterations glucose homeostasis transcriptome and metabolomics in Tgfli1:EGFP zebrafish.       ALDH3A1KO1 GSM8343026   source name:larvae|tissue:larvae|cell line:Tgfli1:EGFP|cell type:larvae|genotype:ALDH3A1KO|geo loc name:missing|collection date:missing ALDH3A1KO1 Raw sequence data with adapter sequences or low quality sequences was filtered. Filtered reads were aligned to the Zebrafish reference genome GRCz11 Raw counts levels were quantified using FeatureCounts v2.0.3 Assembly: GRCz11 Supplementary files format and content: tab delimited text file includes raw counts for each sample. larvae   Total RNA was extracted using RNeasy Mini KitQIAGEN 3ug of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA seq were prepared using DNBSEQ Eukaryotic Strand specific mRNA library.   tissue:larvae|cell line:Tgfli1:EGFP|cell type:larvae|genotype:ALDH3A1KO GSM8343026 GSM8343026: ALDH3A1KO1; Danio rerio; RNA Seq GSM8343026 r1 GSM8343026 1 Total RNA was extracted using RNeasy Mini KitQIAGEN 3ug of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA seq were prepared using DNBSEQ Eukaryotic Strand specific mRNA library.   RNA-Seq TRANSCRIPTOMIC cDNA PAIRED DNBSEQ DNBSEQ-G400   SRP515253     ALDH3A1KO1_1.fq.gz ALDH3A1KO1_2.fq.gz fastq fastq 4801013600.0 24005068.0 GSM8343026 r1 0:100 1:100 A:1307355551;C:1094613295;G:1111765622;T:1287279132;N:0 100 100     1307355551 1094613295 1111765622 1287279132 0 SRX24999614 SRS21700574 SRA1904686 ECAS ECAS                       B B biological fallback assumption bgi bgi unknown cdna_unspecified unknown bulk unknown unknown   Unknown 2024-06-21 Larval Larval Cell Line Cell Line
32880 32880 SRR29488940 SRX24999613 SRS21700573 SRP515253 PRJNA1126644 Elevated MG H1 in glo1 / aldh3a1 / zebrafish caused microvasculature alterations and glucose metabolism impairment via decreased proteasome activity GSE270442 Transcriptome Analysis Dicarbonyl stress is characterized by the abnormal accumulation of dicarbonyl reactive metabolites leading to increased modification of proteins DNA and lipids thereby contributing to cellular and tissue dysfunction in diabetes diabetic complications and other diseases. glo1 knockout zebrafish exhibited moderately increased MG levels yet this was inadequate to induce trunk vessels alterations due to elevated ALDH activity and mRNA expression levels which partially act as compensatory mechanism. Excess 4 HNE induced pancreas dysfunction in aldh3a1 knockout zebrafish larvae inhibiting insulin expression and thereby facilitating hyperglycemia and hyaloid vasculature alterations. To evaluate the combined function of Glo1 and Aldh3a1 in glucose homeostasis and diabetic microvasculature glo1 / aldh3a1 / zebrafish were generated using CRISPR/Cas9 technology. Multiple experiments are performed regarding vasculature alterations glucose homeostasis transcriptome and metabolomics in Tgfli1:EGFP zebrafish. glo1 / aldh3a1 / zebrafish larvae displayed angiogenic hyaloid vasculature caused by the elevated MG H1 and decreased proteasomal chymotrypsin like activity which could be rescued by the MG H1 scavenger L carnosine and proteasome activator Betulinic acid treatments. In adult glo1 / aldh3a1 / zebrafish impaired glucose metabolism angiogenic retina vasculature and thickened GBM were observed. Thus our data suggested that an important upstream factor contributing to these phenomena in the context of lacking glo1 and aldh3a1 is MG H1 possibly through reduced proteasome activity. Overall design: glo1 / aldh3a1 / zebrafish were generated using CRISPR/Cas9 technology. Multiple experiments are performed regarding vasculature alterations glucose homeostasis transcriptome and metabolomics in Tgfli1:EGFP zebrafish.       WT5 GSM8343025   source name:larvae|tissue:larvae|cell line:Tgfli1:EGFP|cell type:larvae|genotype:WT|geo loc name:missing|collection date:missing WT5 Raw sequence data with adapter sequences or low quality sequences was filtered. Filtered reads were aligned to the Zebrafish reference genome GRCz11 Raw counts levels were quantified using FeatureCounts v2.0.3 Assembly: GRCz11 Supplementary files format and content: tab delimited text file includes raw counts for each sample. larvae   Total RNA was extracted using RNeasy Mini KitQIAGEN 3ug of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA seq were prepared using DNBSEQ Eukaryotic Strand specific mRNA library.   tissue:larvae|cell line:Tgfli1:EGFP|cell type:larvae|genotype:WT GSM8343025 GSM8343025: WT5; Danio rerio; RNA Seq GSM8343025 r1 GSM8343025 1 Total RNA was extracted using RNeasy Mini KitQIAGEN 3ug of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA seq were prepared using DNBSEQ Eukaryotic Strand specific mRNA library.   RNA-Seq TRANSCRIPTOMIC cDNA PAIRED DNBSEQ DNBSEQ-G400   SRP515253     WT5_1.fq.gz WT5_2.fq.gz fastq fastq 4820580400.0 24102902.0 GSM8343025 r1 0:100 1:100 A:1288306977;C:1120287477;G:1138943028;T:1273042918;N:0 100 100     1288306977 1120287477 1138943028 1273042918 0 SRX24999613 SRS21700573 SRA1904686 ECAS ECAS                       B B biological fallback assumption bgi bgi unknown cdna_unspecified unknown bulk unknown unknown   Unknown 2024-06-21 Larval Larval Cell Line Cell Line
32881 32881 SRR29488941 SRX24999612 SRS21700572 SRP515253 PRJNA1126644 Elevated MG H1 in glo1 / aldh3a1 / zebrafish caused microvasculature alterations and glucose metabolism impairment via decreased proteasome activity GSE270442 Transcriptome Analysis Dicarbonyl stress is characterized by the abnormal accumulation of dicarbonyl reactive metabolites leading to increased modification of proteins DNA and lipids thereby contributing to cellular and tissue dysfunction in diabetes diabetic complications and other diseases. glo1 knockout zebrafish exhibited moderately increased MG levels yet this was inadequate to induce trunk vessels alterations due to elevated ALDH activity and mRNA expression levels which partially act as compensatory mechanism. Excess 4 HNE induced pancreas dysfunction in aldh3a1 knockout zebrafish larvae inhibiting insulin expression and thereby facilitating hyperglycemia and hyaloid vasculature alterations. To evaluate the combined function of Glo1 and Aldh3a1 in glucose homeostasis and diabetic microvasculature glo1 / aldh3a1 / zebrafish were generated using CRISPR/Cas9 technology. Multiple experiments are performed regarding vasculature alterations glucose homeostasis transcriptome and metabolomics in Tgfli1:EGFP zebrafish. glo1 / aldh3a1 / zebrafish larvae displayed angiogenic hyaloid vasculature caused by the elevated MG H1 and decreased proteasomal chymotrypsin like activity which could be rescued by the MG H1 scavenger L carnosine and proteasome activator Betulinic acid treatments. In adult glo1 / aldh3a1 / zebrafish impaired glucose metabolism angiogenic retina vasculature and thickened GBM were observed. Thus our data suggested that an important upstream factor contributing to these phenomena in the context of lacking glo1 and aldh3a1 is MG H1 possibly through reduced proteasome activity. Overall design: glo1 / aldh3a1 / zebrafish were generated using CRISPR/Cas9 technology. Multiple experiments are performed regarding vasculature alterations glucose homeostasis transcriptome and metabolomics in Tgfli1:EGFP zebrafish.       WT4 GSM8343024   source name:larvae|tissue:larvae|cell line:Tgfli1:EGFP|cell type:larvae|genotype:WT|geo loc name:missing|collection date:missing WT4 Raw sequence data with adapter sequences or low quality sequences was filtered. Filtered reads were aligned to the Zebrafish reference genome GRCz11 Raw counts levels were quantified using FeatureCounts v2.0.3 Assembly: GRCz11 Supplementary files format and content: tab delimited text file includes raw counts for each sample. larvae   Total RNA was extracted using RNeasy Mini KitQIAGEN 3ug of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA seq were prepared using DNBSEQ Eukaryotic Strand specific mRNA library.   tissue:larvae|cell line:Tgfli1:EGFP|cell type:larvae|genotype:WT GSM8343024 GSM8343024: WT4; Danio rerio; RNA Seq GSM8343024 r1 GSM8343024 1 Total RNA was extracted using RNeasy Mini KitQIAGEN 3ug of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA seq were prepared using DNBSEQ Eukaryotic Strand specific mRNA library.   RNA-Seq TRANSCRIPTOMIC cDNA PAIRED DNBSEQ DNBSEQ-G400   SRP515253     WT4_1.fq.gz WT4_2.fq.gz fastq fastq 4821144600.0 24105723.0 GSM8343024 r1 0:100 1:100 A:1275218149;C:1133748961;G:1151831603;T:1260345887;N:0 100 100     1275218149 1133748961 1151831603 1260345887 0 SRX24999612 SRS21700572 SRA1904686 ECAS ECAS                       B B biological fallback assumption bgi bgi unknown cdna_unspecified unknown bulk unknown unknown   Unknown 2024-06-21 Larval Larval Cell Line Cell Line
32882 32882 SRR29488942 SRX24999611 SRS21700571 SRP515253 PRJNA1126644 Elevated MG H1 in glo1 / aldh3a1 / zebrafish caused microvasculature alterations and glucose metabolism impairment via decreased proteasome activity GSE270442 Transcriptome Analysis Dicarbonyl stress is characterized by the abnormal accumulation of dicarbonyl reactive metabolites leading to increased modification of proteins DNA and lipids thereby contributing to cellular and tissue dysfunction in diabetes diabetic complications and other diseases. glo1 knockout zebrafish exhibited moderately increased MG levels yet this was inadequate to induce trunk vessels alterations due to elevated ALDH activity and mRNA expression levels which partially act as compensatory mechanism. Excess 4 HNE induced pancreas dysfunction in aldh3a1 knockout zebrafish larvae inhibiting insulin expression and thereby facilitating hyperglycemia and hyaloid vasculature alterations. To evaluate the combined function of Glo1 and Aldh3a1 in glucose homeostasis and diabetic microvasculature glo1 / aldh3a1 / zebrafish were generated using CRISPR/Cas9 technology. Multiple experiments are performed regarding vasculature alterations glucose homeostasis transcriptome and metabolomics in Tgfli1:EGFP zebrafish. glo1 / aldh3a1 / zebrafish larvae displayed angiogenic hyaloid vasculature caused by the elevated MG H1 and decreased proteasomal chymotrypsin like activity which could be rescued by the MG H1 scavenger L carnosine and proteasome activator Betulinic acid treatments. In adult glo1 / aldh3a1 / zebrafish impaired glucose metabolism angiogenic retina vasculature and thickened GBM were observed. Thus our data suggested that an important upstream factor contributing to these phenomena in the context of lacking glo1 and aldh3a1 is MG H1 possibly through reduced proteasome activity. Overall design: glo1 / aldh3a1 / zebrafish were generated using CRISPR/Cas9 technology. Multiple experiments are performed regarding vasculature alterations glucose homeostasis transcriptome and metabolomics in Tgfli1:EGFP zebrafish.       WT3 GSM8343023   source name:larvae|tissue:larvae|cell line:Tgfli1:EGFP|cell type:larvae|genotype:WT|geo loc name:missing|collection date:missing WT3 Raw sequence data with adapter sequences or low quality sequences was filtered. Filtered reads were aligned to the Zebrafish reference genome GRCz11 Raw counts levels were quantified using FeatureCounts v2.0.3 Assembly: GRCz11 Supplementary files format and content: tab delimited text file includes raw counts for each sample. larvae   Total RNA was extracted using RNeasy Mini KitQIAGEN 3ug of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA seq were prepared using DNBSEQ Eukaryotic Strand specific mRNA library.   tissue:larvae|cell line:Tgfli1:EGFP|cell type:larvae|genotype:WT GSM8343023 GSM8343023: WT3; Danio rerio; RNA Seq GSM8343023 r1 GSM8343023 1 Total RNA was extracted using RNeasy Mini KitQIAGEN 3ug of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA seq were prepared using DNBSEQ Eukaryotic Strand specific mRNA library.   RNA-Seq TRANSCRIPTOMIC cDNA PAIRED DNBSEQ DNBSEQ-G400   SRP515253     WT3_1.fq.gz WT3_2.fq.gz fastq fastq 4826072600.0 24130363.0 GSM8343023 r1 0:100 1:100 A:1278424901;C:1132200743;G:1150406212;T:1265040744;N:0 100 100     1278424901 1132200743 1150406212 1265040744 0 SRX24999611 SRS21700571 SRA1904686 ECAS ECAS                       B B biological fallback assumption bgi bgi unknown cdna_unspecified unknown bulk unknown unknown   Unknown 2024-06-21 Larval Larval Cell Line Cell Line
32883 32883 SRR29488943 SRX24999610 SRS21700570 SRP515253 PRJNA1126644 Elevated MG H1 in glo1 / aldh3a1 / zebrafish caused microvasculature alterations and glucose metabolism impairment via decreased proteasome activity GSE270442 Transcriptome Analysis Dicarbonyl stress is characterized by the abnormal accumulation of dicarbonyl reactive metabolites leading to increased modification of proteins DNA and lipids thereby contributing to cellular and tissue dysfunction in diabetes diabetic complications and other diseases. glo1 knockout zebrafish exhibited moderately increased MG levels yet this was inadequate to induce trunk vessels alterations due to elevated ALDH activity and mRNA expression levels which partially act as compensatory mechanism. Excess 4 HNE induced pancreas dysfunction in aldh3a1 knockout zebrafish larvae inhibiting insulin expression and thereby facilitating hyperglycemia and hyaloid vasculature alterations. To evaluate the combined function of Glo1 and Aldh3a1 in glucose homeostasis and diabetic microvasculature glo1 / aldh3a1 / zebrafish were generated using CRISPR/Cas9 technology. Multiple experiments are performed regarding vasculature alterations glucose homeostasis transcriptome and metabolomics in Tgfli1:EGFP zebrafish. glo1 / aldh3a1 / zebrafish larvae displayed angiogenic hyaloid vasculature caused by the elevated MG H1 and decreased proteasomal chymotrypsin like activity which could be rescued by the MG H1 scavenger L carnosine and proteasome activator Betulinic acid treatments. In adult glo1 / aldh3a1 / zebrafish impaired glucose metabolism angiogenic retina vasculature and thickened GBM were observed. Thus our data suggested that an important upstream factor contributing to these phenomena in the context of lacking glo1 and aldh3a1 is MG H1 possibly through reduced proteasome activity. Overall design: glo1 / aldh3a1 / zebrafish were generated using CRISPR/Cas9 technology. Multiple experiments are performed regarding vasculature alterations glucose homeostasis transcriptome and metabolomics in Tgfli1:EGFP zebrafish.       WT2 GSM8343022   source name:larvae|tissue:larvae|cell line:Tgfli1:EGFP|cell type:larvae|genotype:WT|geo loc name:missing|collection date:missing WT2 Raw sequence data with adapter sequences or low quality sequences was filtered. Filtered reads were aligned to the Zebrafish reference genome GRCz11 Raw counts levels were quantified using FeatureCounts v2.0.3 Assembly: GRCz11 Supplementary files format and content: tab delimited text file includes raw counts for each sample. larvae   Total RNA was extracted using RNeasy Mini KitQIAGEN 3ug of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA seq were prepared using DNBSEQ Eukaryotic Strand specific mRNA library.   tissue:larvae|cell line:Tgfli1:EGFP|cell type:larvae|genotype:WT GSM8343022 GSM8343022: WT2; Danio rerio; RNA Seq GSM8343022 r1 GSM8343022 1 Total RNA was extracted using RNeasy Mini KitQIAGEN 3ug of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA seq were prepared using DNBSEQ Eukaryotic Strand specific mRNA library.   RNA-Seq TRANSCRIPTOMIC cDNA PAIRED DNBSEQ DNBSEQ-G400   SRP515253     WT2_1.fq.gz WT2_2.fq.gz fastq fastq 4801181000.0 24005905.0 GSM8343022 r1 0:100 1:100 A:1271169177;C:1127438892;G:1145437659;T:1257135272;N:0 100 100     1271169177 1127438892 1145437659 1257135272 0 SRX24999610 SRS21700570 SRA1904686 ECAS ECAS                       B B biological fallback assumption bgi bgi unknown cdna_unspecified unknown bulk unknown unknown   Unknown 2024-06-21 Larval Larval Cell Line Cell Line
32884 32884 SRR29488944 SRX24999609 SRS21700569 SRP515253 PRJNA1126644 Elevated MG H1 in glo1 / aldh3a1 / zebrafish caused microvasculature alterations and glucose metabolism impairment via decreased proteasome activity GSE270442 Transcriptome Analysis Dicarbonyl stress is characterized by the abnormal accumulation of dicarbonyl reactive metabolites leading to increased modification of proteins DNA and lipids thereby contributing to cellular and tissue dysfunction in diabetes diabetic complications and other diseases. glo1 knockout zebrafish exhibited moderately increased MG levels yet this was inadequate to induce trunk vessels alterations due to elevated ALDH activity and mRNA expression levels which partially act as compensatory mechanism. Excess 4 HNE induced pancreas dysfunction in aldh3a1 knockout zebrafish larvae inhibiting insulin expression and thereby facilitating hyperglycemia and hyaloid vasculature alterations. To evaluate the combined function of Glo1 and Aldh3a1 in glucose homeostasis and diabetic microvasculature glo1 / aldh3a1 / zebrafish were generated using CRISPR/Cas9 technology. Multiple experiments are performed regarding vasculature alterations glucose homeostasis transcriptome and metabolomics in Tgfli1:EGFP zebrafish. glo1 / aldh3a1 / zebrafish larvae displayed angiogenic hyaloid vasculature caused by the elevated MG H1 and decreased proteasomal chymotrypsin like activity which could be rescued by the MG H1 scavenger L carnosine and proteasome activator Betulinic acid treatments. In adult glo1 / aldh3a1 / zebrafish impaired glucose metabolism angiogenic retina vasculature and thickened GBM were observed. Thus our data suggested that an important upstream factor contributing to these phenomena in the context of lacking glo1 and aldh3a1 is MG H1 possibly through reduced proteasome activity. Overall design: glo1 / aldh3a1 / zebrafish were generated using CRISPR/Cas9 technology. Multiple experiments are performed regarding vasculature alterations glucose homeostasis transcriptome and metabolomics in Tgfli1:EGFP zebrafish.       WT1 GSM8343021   source name:larvae|tissue:larvae|cell line:Tgfli1:EGFP|cell type:larvae|genotype:WT|geo loc name:missing|collection date:missing WT1 Raw sequence data with adapter sequences or low quality sequences was filtered. Filtered reads were aligned to the Zebrafish reference genome GRCz11 Raw counts levels were quantified using FeatureCounts v2.0.3 Assembly: GRCz11 Supplementary files format and content: tab delimited text file includes raw counts for each sample. larvae   Total RNA was extracted using RNeasy Mini KitQIAGEN 3ug of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA seq were prepared using DNBSEQ Eukaryotic Strand specific mRNA library.   tissue:larvae|cell line:Tgfli1:EGFP|cell type:larvae|genotype:WT GSM8343021 GSM8343021: WT1; Danio rerio; RNA Seq GSM8343021 r1 GSM8343021 1 Total RNA was extracted using RNeasy Mini KitQIAGEN 3ug of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA seq were prepared using DNBSEQ Eukaryotic Strand specific mRNA library.   RNA-Seq TRANSCRIPTOMIC cDNA PAIRED DNBSEQ DNBSEQ-G400   SRP515253     WT1_1.fq.gz WT1_2.fq.gz fastq fastq 4802502800.0 24012514.0 GSM8343021 r1 0:100 1:100 A:1263416018;C:1135581393;G:1152700487;T:1250804902;N:0 100 100     1263416018 1135581393 1152700487 1250804902 0 SRX24999609 SRS21700569 SRA1904686 ECAS ECAS                       B B biological fallback assumption bgi bgi unknown cdna_unspecified unknown bulk unknown unknown   Unknown 2024-06-21 Larval Larval Cell Line Cell Line
33982 33982 SRR31022893 SRX26409990 SRS22929902 SRP539058 PRJNA1173962 Study on the changes of gene transcription levels in zebrafish larvae treated to prothiobacillazole solution PRJNA1173962 Other ProthiobacillazolePTA is a kind of widely used bactericidal pesticide. Due to its high water solubility and more negative reports on aquatic organisms in recent yrs it is necessary to further study its environment.Zebrafish as an in vivo research model has its unique advantages in evaluating drug toxicity and safety as it can efficiently evaluate multiple organ toxicities at the same time. In this study the PTA exposed and Control zebrafish larvae were collected at xxxdpf for each group of 4 replicates.The sample is used for RNA seq.     Repeat four:the PTA solution was exposed and cultured ttwo xxxdpf PTA PTA4   strain:not applicable|isolate:missing|breed:missing|cultivar:not applicable|ecotype:missing|age:4dpf stage:larval phase|collection date:2024 04|geo loc name:missing|sex:missing|tissue:missing|biomaterial provider:Fish stress response and immune response Research team|birth date:missing|birth location:missing|breeding history:missing|breeding method:missing|cell line:not applicable|cell subtype:missing|cell type:missing|collected by:ChenGuo|culture collection:missing|death date:missing|disease:missing|disease stg:missing|genotype:missing|growth protocol:missing|health state:health|isolation source:missing|lat lon:missing|phenotype:missing|sample type:missing|specimen voucher:missing|store cond:missing|stud book number:missing|treatment:missing|costumer description:8|BioSampleModel:Model organism or animal                 RNA seq of zebrafish PTA4 PTA4 Use the Invitrogen TRIzol kit to extract total RNA. Select total RNA with a total amount of at least 1ug and use the NEBNext Ultra II RNA Library Prep Kit for Illumina New England Biolabs Inc; Ipswich Massachusetts USA to construct sequencing libraries.     RNA-Seq TRANSCRIPTOMIC cDNA PAIRED ILLUMINA Illumina NovaSeq 6000   SRP539058     PTA4_R1.fastq.gz PTA4_R2.fastq.gz fastq fastq 8283122214.0 27427557.0 PTA4 R1.fastq.gz 0:151 1:151 A:2240001804;C:1891821306;G:1934930549;T:2215819380;N:549175 151 151     2240001804 1891821306 1934930549 2215819380 549175 SRX26409990 SRS22929902 SRA1992822 Nanchang University|College of Life Science Nanchang University                       B B biological fallback assumption illumina novaseq_era unknown cdna_unspecified nebnext bulk unknown unknown   China 2024-10-17 Larval Larval Cell Line Cell Line
33983 33983 SRR31022894 SRX26409989 SRS22929901 SRP539058 PRJNA1173962 Study on the changes of gene transcription levels in zebrafish larvae treated to prothiobacillazole solution PRJNA1173962 Other ProthiobacillazolePTA is a kind of widely used bactericidal pesticide. Due to its high water solubility and more negative reports on aquatic organisms in recent yrs it is necessary to further study its environment.Zebrafish as an in vivo research model has its unique advantages in evaluating drug toxicity and safety as it can efficiently evaluate multiple organ toxicities at the same time. In this study the PTA exposed and Control zebrafish larvae were collected at xxxdpf for each group of 4 replicates.The sample is used for RNA seq.     Repeat three:the PTA solution was exposed and cultured ttwo xxxdpf PTA PTA3   strain:not applicable|isolate:missing|breed:missing|cultivar:not applicable|ecotype:missing|age:4dpf stage:larval phase|collection date:2024 04|geo loc name:missing|sex:missing|tissue:missing|biomaterial provider:Fish stress response and immune response Research team|birth date:missing|birth location:missing|breeding history:missing|breeding method:missing|cell line:not applicable|cell subtype:missing|cell type:missing|collected by:ChenGuo|culture collection:missing|death date:missing|disease:missing|disease stg:missing|genotype:missing|growth protocol:missing|health state:health|isolation source:missing|lat lon:missing|phenotype:missing|sample type:missing|specimen voucher:missing|store cond:missing|stud book number:missing|treatment:missing|costumer description:7|BioSampleModel:Model organism or animal                 RNA seq of zebrafish PTA3 PTA3 Use the Invitrogen TRIzol kit to extract total RNA. Select total RNA with a total amount of at least 1ug and use the NEBNext Ultra II RNA Library Prep Kit for Illumina New England Biolabs Inc; Ipswich Massachusetts USA to construct sequencing libraries.     RNA-Seq TRANSCRIPTOMIC cDNA PAIRED ILLUMINA Illumina NovaSeq 6000   SRP539058     PTA3_R1.fastq.gz PTA3_R2.fastq.gz fastq fastq 7022708604.0 23254002.0 PTA3 R1.fastq.gz 0:151 1:151 A:1891728501;C:1605352134;G:1649370849;T:1875796526;N:460594 151 151     1891728501 1605352134 1649370849 1875796526 460594 SRX26409989 SRS22929901 SRA1992822 Nanchang University|College of Life Science Nanchang University                       B B biological fallback assumption illumina novaseq_era unknown cdna_unspecified nebnext bulk unknown unknown   China 2024-10-17 Larval Larval Cell Line Cell Line
33984 33984 SRR31022895 SRX26409988 SRS22929900 SRP539058 PRJNA1173962 Study on the changes of gene transcription levels in zebrafish larvae treated to prothiobacillazole solution PRJNA1173962 Other ProthiobacillazolePTA is a kind of widely used bactericidal pesticide. Due to its high water solubility and more negative reports on aquatic organisms in recent yrs it is necessary to further study its environment.Zebrafish as an in vivo research model has its unique advantages in evaluating drug toxicity and safety as it can efficiently evaluate multiple organ toxicities at the same time. In this study the PTA exposed and Control zebrafish larvae were collected at xxxdpf for each group of 4 replicates.The sample is used for RNA seq.     Repeat two:the PTA solution was exposed and cultured ttwo xxxdpf PTA PTA2   strain:not applicable|isolate:missing|breed:missing|cultivar:not applicable|ecotype:missing|age:4dpf stage:larval phase|collection date:2024 04|geo loc name:missing|sex:missing|tissue:missing|biomaterial provider:Fish stress response and immune response Research team|birth date:missing|birth location:missing|breeding history:missing|breeding method:missing|cell line:not applicable|cell subtype:missing|cell type:missing|collected by:ChenGuo|culture collection:missing|death date:missing|disease:missing|disease stg:missing|genotype:missing|growth protocol:missing|health state:health|isolation source:missing|lat lon:missing|phenotype:missing|sample type:missing|specimen voucher:missing|store cond:missing|stud book number:missing|treatment:missing|costumer description:6|BioSampleModel:Model organism or animal                 RNA seq of zebrafish PTA2 PTA2 Use the Invitrogen TRIzol kit to extract total RNA. Select total RNA with a total amount of at least 1ug and use the NEBNext Ultra II RNA Library Prep Kit for Illumina New England Biolabs Inc; Ipswich Massachusetts USA to construct sequencing libraries.     RNA-Seq TRANSCRIPTOMIC cDNA PAIRED ILLUMINA Illumina NovaSeq 6000   SRP539058     PTA2_R1.fastq.gz PTA2_R2.fastq.gz fastq fastq 7096338016.0 23497808.0 PTA2 R1.fastq.gz 0:151 1:151 A:1934868395;C:1599522000;G:1643956349;T:1917509746;N:481526 151 151     1934868395 1599522000 1643956349 1917509746 481526 SRX26409988 SRS22929900 SRA1992822 Nanchang University|College of Life Science Nanchang University                       B B biological fallback assumption illumina novaseq_era unknown cdna_unspecified nebnext bulk unknown unknown   China 2024-10-17 Larval Larval Cell Line Cell Line
33985 33985 SRR31022896 SRX26409987 SRS22929899 SRP539058 PRJNA1173962 Study on the changes of gene transcription levels in zebrafish larvae treated to prothiobacillazole solution PRJNA1173962 Other ProthiobacillazolePTA is a kind of widely used bactericidal pesticide. Due to its high water solubility and more negative reports on aquatic organisms in recent yrs it is necessary to further study its environment.Zebrafish as an in vivo research model has its unique advantages in evaluating drug toxicity and safety as it can efficiently evaluate multiple organ toxicities at the same time. In this study the PTA exposed and Control zebrafish larvae were collected at xxxdpf for each group of 4 replicates.The sample is used for RNA seq.     Repeat one:the PTA solution was exposed and cultured ttwo xxxdpf PTA PTA1   strain:not applicable|isolate:missing|breed:missing|cultivar:not applicable|ecotype:missing|age:4dpf stage:larval phase|collection date:2024 04|geo loc name:missing|sex:missing|tissue:missing|biomaterial provider:Fish stress response and immune response Research team|birth date:missing|birth location:missing|breeding history:missing|breeding method:missing|cell line:not applicable|cell subtype:missing|cell type:missing|collected by:ChenGuo|culture collection:missing|death date:missing|disease:missing|disease stg:missing|genotype:missing|growth protocol:missing|health state:health|isolation source:missing|lat lon:missing|phenotype:missing|sample type:missing|specimen voucher:missing|store cond:missing|stud book number:missing|treatment:missing|costumer description:5|BioSampleModel:Model organism or animal                 RNA seq of zebrafish PTA1 PTA1 Use the Invitrogen TRIzol kit to extract total RNA. Select total RNA with a total amount of at least 1ug and use the NEBNext Ultra II RNA Library Prep Kit for Illumina New England Biolabs Inc; Ipswich Massachusetts USA to construct sequencing libraries.     RNA-Seq TRANSCRIPTOMIC cDNA PAIRED ILLUMINA Illumina NovaSeq 6000   SRP539058     PTA1_R1.fastq.gz PTA1_R2.fastq.gz fastq fastq 7610150246.0 25199173.0 PTA1 R1.fastq.gz 0:151 1:151 A:2078407024;C:1716455571;G:1761054093;T:2053740332;N:493226 151 151     2078407024 1716455571 1761054093 2053740332 493226 SRX26409987 SRS22929899 SRA1992822 Nanchang University|College of Life Science Nanchang University                       B B biological fallback assumption illumina novaseq_era unknown cdna_unspecified nebnext bulk unknown unknown   China 2024-10-17 Larval Larval Cell Line Cell Line

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CREATE TABLE run_metadata("run.accession" VARCHAR, "experiment.accession" VARCHAR, "sample.accession" VARCHAR, "study.accession" VARCHAR, bioproject VARCHAR, "study.title" VARCHAR, "study.alias" VARCHAR, "study.type" VARCHAR, "study.abstract" VARCHAR, "study.attributes" VARCHAR, "study.PMIDs" VARCHAR, "sample.description" VARCHAR, "sample.title" VARCHAR, "sample.alias" VARCHAR, "sample.centername" VARCHAR, "sample.attributes" VARCHAR, "GEOsample.title" VARCHAR, "GEOsample.dataprocessing" VARCHAR, "GEOsample.source" VARCHAR, "GEOsample.treatmentprotocol" VARCHAR, "GEOsample.extractprotocol" VARCHAR, "GEOsample.growthprotocol" VARCHAR, "GEOsample.characteristics" VARCHAR, "GEOsample.accession" VARCHAR, "experiment.title" VARCHAR, "experiment.alias" VARCHAR, "experiment.library_name" VARCHAR, "experiment.design_description" VARCHAR, "experiment.library_construction_protocol" VARCHAR, "experiment.attributes" VARCHAR, "experiment.library_strategy" VARCHAR, "experiment.library_source" VARCHAR, "experiment.library_selection" VARCHAR, "experiment.library_layout" VARCHAR, "experiment.platform" VARCHAR, "experiment.instrument_model" VARCHAR, "experiment.spot_descriptor" VARCHAR, "experiment.study_ref" VARCHAR, "run.title" VARCHAR, "run.attributes" VARCHAR, "run.filename" VARCHAR, "run.semantic_name" VARCHAR, "run.total_bases" DOUBLE, "run.total_spots" DOUBLE, "run.alias" VARCHAR, "run.read_lengths" VARCHAR, "run.base_counts" VARCHAR, "run.r1_length" BIGINT, "run.r2_length" BIGINT, "run.r3_length" BIGINT, "run.r4_length" BIGINT, "run.Acount" BIGINT, "run.Ccount" BIGINT, "run.Gcount" BIGINT, "run.Tcount" BIGINT, "run.Ncount" BIGINT, "run.experiment" VARCHAR, "run.pool_member" VARCHAR, "submission.accession" VARCHAR, "submission.srasource" VARCHAR, "submission.bioprojectsource" VARCHAR, "seqdetective.n_mates" BIGINT, "seqdetective.mapping_rate.mate1" DOUBLE, "seqdetective.mapping_rate.mate2" DOUBLE, "seqdetective.nofeature_rate.mate1" DOUBLE, "seqdetective.nofeature_rate.mate2" DOUBLE, "seqdetective.sparsity.mate1" DOUBLE, "seqdetective.sparsity.mate2" DOUBLE, "seqdetective.pos_strand_rate.mate1" DOUBLE, "seqdetective.pos_strand_rate.mate2" DOUBLE, "seqdetective.readlen.mate1" BIGINT, "seqdetective.readlen.mate2" BIGINT, "seqdetective.judgement.mate1" VARCHAR, "seqdetective.judgement.mate2" VARCHAR, "seqdetective.judgement.reason" VARCHAR, platform_family VARCHAR, instrument_generation VARCHAR, read_bias VARCHAR, selection_class VARCHAR, prep_kit VARCHAR, sc_or_bulk VARCHAR, tech_class VARCHAR, technology VARCHAR, tech_variant VARCHAR, "submission.bioprojectsource.country" VARCHAR, earliest_date DATE, devstage_curation VARCHAR, devstage_curation_coarse VARCHAR, tissue_curation VARCHAR, tissue_curation_coarse VARCHAR);;
CREATE INDEX idx_run_bioproject ON run_metadata(bioproject);;
CREATE INDEX idx_run_run_accession ON run_metadata("run.accession");;