run_metadata
38 rows where devstage_curation_coarse = "Juvenile" and experiment.library_selection = "RT-PCR"
This data as json, CSV (advanced)
| Link | rowid ▼ | run.accession | experiment.accession | sample.accession | study.accession | bioproject | study.title | study.alias | study.type | study.abstract | study.attributes | study.PMIDs | sample.description | sample.title | sample.alias | sample.centername | sample.attributes | GEOsample.title | GEOsample.dataprocessing | GEOsample.source | GEOsample.treatmentprotocol | GEOsample.extractprotocol | GEOsample.growthprotocol | GEOsample.characteristics | GEOsample.accession | experiment.title | experiment.alias | experiment.library_name | experiment.design_description | experiment.library_construction_protocol | experiment.attributes | experiment.library_strategy | experiment.library_source | experiment.library_selection | experiment.library_layout | experiment.platform | experiment.instrument_model | experiment.spot_descriptor | experiment.study_ref | run.title | run.attributes | run.filename | run.semantic_name | run.total_bases | run.total_spots | run.alias | run.read_lengths | run.base_counts | run.r1_length | run.r2_length | run.r3_length | run.r4_length | run.Acount | run.Ccount | run.Gcount | run.Tcount | run.Ncount | run.experiment | run.pool_member | submission.accession | submission.srasource | submission.bioprojectsource | seqdetective.n_mates | seqdetective.mapping_rate.mate1 | seqdetective.mapping_rate.mate2 | seqdetective.nofeature_rate.mate1 | seqdetective.nofeature_rate.mate2 | seqdetective.sparsity.mate1 | seqdetective.sparsity.mate2 | seqdetective.pos_strand_rate.mate1 | seqdetective.pos_strand_rate.mate2 | seqdetective.readlen.mate1 | seqdetective.readlen.mate2 | seqdetective.judgement.mate1 | seqdetective.judgement.mate2 | seqdetective.judgement.reason | platform_family | instrument_generation | read_bias | selection_class | prep_kit | sc_or_bulk | tech_class | technology | tech_variant | submission.bioprojectsource.country | earliest_date | devstage_curation | devstage_curation_coarse | tissue_curation | tissue_curation_coarse |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 34128 | 34128 | SRR31371655 | SRX26745254 | SRS23234144 | SRP545712 | PRJNA1186363 | Danio rerio Transcriptome or Gene expression | PRJNA1186363 | Other | Gonad tissue of juvenile zebrafish post long term treatment with nano copper or melatonin | Model organism or animal sample from zebrafish | zebra001 | cultivar:zebrafish|age:40dpf|collection date:2024 11 15|geo loc name:China|sex:female|tissue:Freshwater Fisheries Research Center of ChineseAcademy of Fishery Sciences|zebrafish:Gonadal development|BioSampleModel:Model organism or animal | RNA seq from the gonad of Zebrafish | PC3 R1.fq | PC3 R1.fq | CuNPs and melatonin treatment | RNA-Seq | TRANSCRIPTOMIC | RT-PCR | PAIRED | ILLUMINA | Illumina NovaSeq X Plus | SRP545712 | PC3_R1.fq.gz PC3_R2.fq.gz | fastq fastq | 8050557450.0 | 26657475.0 | PC3 R1.fq.gz | SRX26745254 | SRS23234144 | SRA2016483 | Chinese Academy of Fishery Science|Freshwater Fishery Research Center | Chinese Academy of Fishery Science | B | B | biological fallback assumption | illumina | novaseq_era | unknown | other | unknown | bulk | unknown | unknown | China | 2024-11-17 | Juvenile | Juvenile | Gonad | Reproductive System | ||||||||||||||||||||||||||||||||||||||||
| 34129 | 34129 | SRR31371656 | SRX26745253 | SRS23234144 | SRP545712 | PRJNA1186363 | Danio rerio Transcriptome or Gene expression | PRJNA1186363 | Other | Gonad tissue of juvenile zebrafish post long term treatment with nano copper or melatonin | Model organism or animal sample from zebrafish | zebra001 | cultivar:zebrafish|age:40dpf|collection date:2024 11 15|geo loc name:China|sex:female|tissue:Freshwater Fisheries Research Center of ChineseAcademy of Fishery Sciences|zebrafish:Gonadal development|BioSampleModel:Model organism or animal | RNA seq from the gonad of Zebrafish | PC2 R1.fq | PC2 R1.fq | CuNPs and melatonin treatment | RNA-Seq | TRANSCRIPTOMIC | RT-PCR | PAIRED | ILLUMINA | Illumina NovaSeq X Plus | SRP545712 | PC2_R1.fq.gz PC2_R2.fq.gz | fastq fastq | 7072903722.0 | 23420211.0 | PC2 R1.fq.gz | 0:151 1:151 | A:1848366962;C:1685927772;G:1702568524;T:1835518466;N:521998 | 151 | 151 | 1848366962 | 1685927772 | 1702568524 | 1835518466 | 521998 | SRX26745253 | SRS23234144 | SRA2016483 | Chinese Academy of Fishery Science|Freshwater Fishery Research Center | Chinese Academy of Fishery Science | B | B | biological fallback assumption | illumina | novaseq_era | unknown | other | unknown | bulk | unknown | unknown | China | 2024-11-17 | Juvenile | Juvenile | Gonad | Reproductive System | |||||||||||||||||||||||||||||||
| 34130 | 34130 | SRR31371657 | SRX26745252 | SRS23234144 | SRP545712 | PRJNA1186363 | Danio rerio Transcriptome or Gene expression | PRJNA1186363 | Other | Gonad tissue of juvenile zebrafish post long term treatment with nano copper or melatonin | Model organism or animal sample from zebrafish | zebra001 | cultivar:zebrafish|age:40dpf|collection date:2024 11 15|geo loc name:China|sex:female|tissue:Freshwater Fisheries Research Center of ChineseAcademy of Fishery Sciences|zebrafish:Gonadal development|BioSampleModel:Model organism or animal | RNA seq from the gonad of Zebrafish | PC1 R1.fq | PC1 R1.fq | CuNPs and melatonin treatment | RNA-Seq | TRANSCRIPTOMIC | RT-PCR | PAIRED | ILLUMINA | Illumina NovaSeq X Plus | SRP545712 | PC1_R1.fq.gz PC1_R2.fq.gz | fastq fastq | 9551575668.0 | 31627734.0 | PC1 R1.fq.gz | SRX26745252 | SRS23234144 | SRA2016483 | Chinese Academy of Fishery Science|Freshwater Fishery Research Center | Chinese Academy of Fishery Science | B | B | biological fallback assumption | illumina | novaseq_era | unknown | other | unknown | bulk | unknown | unknown | China | 2024-11-17 | Juvenile | Juvenile | Gonad | Reproductive System | ||||||||||||||||||||||||||||||||||||||||
| 34131 | 34131 | SRR31371658 | SRX26745251 | SRS23234144 | SRP545712 | PRJNA1186363 | Danio rerio Transcriptome or Gene expression | PRJNA1186363 | Other | Gonad tissue of juvenile zebrafish post long term treatment with nano copper or melatonin | Model organism or animal sample from zebrafish | zebra001 | cultivar:zebrafish|age:40dpf|collection date:2024 11 15|geo loc name:China|sex:female|tissue:Freshwater Fisheries Research Center of ChineseAcademy of Fishery Sciences|zebrafish:Gonadal development|BioSampleModel:Model organism or animal | RNA seq from the gonad of Zebrafish | PB3 R1.fq | PB3 R1.fq | CuNPs treatment | RNA-Seq | TRANSCRIPTOMIC | RT-PCR | PAIRED | ILLUMINA | Illumina NovaSeq X Plus | SRP545712 | PB3_R1.fq.gz PB3_R2.fq.gz | fastq fastq | 6839092000.0 | 22646000.0 | PB3 R1.fq.gz | SRX26745251 | SRS23234144 | SRA2016483 | Chinese Academy of Fishery Science|Freshwater Fishery Research Center | Chinese Academy of Fishery Science | B | B | biological fallback assumption | illumina | novaseq_era | unknown | other | unknown | bulk | unknown | unknown | China | 2024-11-17 | Juvenile | Juvenile | Gonad | Reproductive System | ||||||||||||||||||||||||||||||||||||||||
| 34132 | 34132 | SRR31371659 | SRX26745250 | SRS23234144 | SRP545712 | PRJNA1186363 | Danio rerio Transcriptome or Gene expression | PRJNA1186363 | Other | Gonad tissue of juvenile zebrafish post long term treatment with nano copper or melatonin | Model organism or animal sample from zebrafish | zebra001 | cultivar:zebrafish|age:40dpf|collection date:2024 11 15|geo loc name:China|sex:female|tissue:Freshwater Fisheries Research Center of ChineseAcademy of Fishery Sciences|zebrafish:Gonadal development|BioSampleModel:Model organism or animal | RNA seq from the gonad of Zebrafish | PB2 R1.fq | PB2 R1.fq | CuNPs treatment | RNA-Seq | TRANSCRIPTOMIC | RT-PCR | PAIRED | ILLUMINA | Illumina NovaSeq X Plus | SRP545712 | PB2_R1.fq.gz PB2_R2.fq.gz | fastq fastq | 7582292558.0 | 25106929.0 | PB2 R1.fq.gz | SRX26745250 | SRS23234144 | SRA2016483 | Chinese Academy of Fishery Science|Freshwater Fishery Research Center | Chinese Academy of Fishery Science | B | B | biological fallback assumption | illumina | novaseq_era | unknown | other | unknown | bulk | unknown | unknown | China | 2024-11-17 | Juvenile | Juvenile | Gonad | Reproductive System | ||||||||||||||||||||||||||||||||||||||||
| 34133 | 34133 | SRR31371660 | SRX26745249 | SRS23234144 | SRP545712 | PRJNA1186363 | Danio rerio Transcriptome or Gene expression | PRJNA1186363 | Other | Gonad tissue of juvenile zebrafish post long term treatment with nano copper or melatonin | Model organism or animal sample from zebrafish | zebra001 | cultivar:zebrafish|age:40dpf|collection date:2024 11 15|geo loc name:China|sex:female|tissue:Freshwater Fisheries Research Center of ChineseAcademy of Fishery Sciences|zebrafish:Gonadal development|BioSampleModel:Model organism or animal | RNA seq from the gonad of Zebrafish | PB1 R1.fq | PB1 R1.fq | CuNPs treatment | RNA-Seq | TRANSCRIPTOMIC | RT-PCR | PAIRED | ILLUMINA | Illumina NovaSeq X Plus | SRP545712 | PB1_R1.fq.gz PB1_R2.fq.gz | fastq fastq | 8122972218.0 | 26897259.0 | PB1 R1.fq.gz | SRX26745249 | SRS23234144 | SRA2016483 | Chinese Academy of Fishery Science|Freshwater Fishery Research Center | Chinese Academy of Fishery Science | B | B | biological fallback assumption | illumina | novaseq_era | unknown | other | unknown | bulk | unknown | unknown | China | 2024-11-17 | Juvenile | Juvenile | Gonad | Reproductive System | ||||||||||||||||||||||||||||||||||||||||
| 34134 | 34134 | SRR31371661 | SRX26745248 | SRS23234144 | SRP545712 | PRJNA1186363 | Danio rerio Transcriptome or Gene expression | PRJNA1186363 | Other | Gonad tissue of juvenile zebrafish post long term treatment with nano copper or melatonin | Model organism or animal sample from zebrafish | zebra001 | cultivar:zebrafish|age:40dpf|collection date:2024 11 15|geo loc name:China|sex:female|tissue:Freshwater Fisheries Research Center of ChineseAcademy of Fishery Sciences|zebrafish:Gonadal development|BioSampleModel:Model organism or animal | RNA seq from the gonad of Zebrafish | CA3 R1.fq | CA3 R1.fq | control | RNA-Seq | TRANSCRIPTOMIC | RT-PCR | PAIRED | ILLUMINA | Illumina NovaSeq X Plus | SRP545712 | CA3_R1.fq.gz CA3_R2.fq.gz | fastq fastq | 7638136586.0 | 25291843.0 | CA3 R1.fq.gz | SRX26745248 | SRS23234144 | SRA2016483 | Chinese Academy of Fishery Science|Freshwater Fishery Research Center | Chinese Academy of Fishery Science | B | B | biological fallback assumption | illumina | novaseq_era | unknown | other | unknown | bulk | unknown | unknown | China | 2024-11-17 | Juvenile | Juvenile | Gonad | Reproductive System | ||||||||||||||||||||||||||||||||||||||||
| 34135 | 34135 | SRR31371662 | SRX26745247 | SRS23234144 | SRP545712 | PRJNA1186363 | Danio rerio Transcriptome or Gene expression | PRJNA1186363 | Other | Gonad tissue of juvenile zebrafish post long term treatment with nano copper or melatonin | Model organism or animal sample from zebrafish | zebra001 | cultivar:zebrafish|age:40dpf|collection date:2024 11 15|geo loc name:China|sex:female|tissue:Freshwater Fisheries Research Center of ChineseAcademy of Fishery Sciences|zebrafish:Gonadal development|BioSampleModel:Model organism or animal | RNA seq from the gonad of Zebrafish | CA1 R1.fq | CA1 R1.fq | control | RNA-Seq | TRANSCRIPTOMIC | RT-PCR | PAIRED | ILLUMINA | Illumina NovaSeq X Plus | SRP545712 | CA1_R1.fq.gz CA1_R2.fq.gz | fastq fastq | 7499744784.0 | 24833592.0 | CA1 R1.fq.gz | 0:151 1:151 | A:1951636355;C:1801626786;G:1812560367;T:1933366696;N:554580 | 151 | 151 | 1951636355 | 1801626786 | 1812560367 | 1933366696 | 554580 | SRX26745247 | SRS23234144 | SRA2016483 | Chinese Academy of Fishery Science|Freshwater Fishery Research Center | Chinese Academy of Fishery Science | B | B | biological fallback assumption | illumina | novaseq_era | unknown | other | unknown | bulk | unknown | unknown | China | 2024-11-17 | Juvenile | Juvenile | Gonad | Reproductive System | |||||||||||||||||||||||||||||||
| 34136 | 34136 | SRR31371663 | SRX26745246 | SRS23234144 | SRP545712 | PRJNA1186363 | Danio rerio Transcriptome or Gene expression | PRJNA1186363 | Other | Gonad tissue of juvenile zebrafish post long term treatment with nano copper or melatonin | Model organism or animal sample from zebrafish | zebra001 | cultivar:zebrafish|age:40dpf|collection date:2024 11 15|geo loc name:China|sex:female|tissue:Freshwater Fisheries Research Center of ChineseAcademy of Fishery Sciences|zebrafish:Gonadal development|BioSampleModel:Model organism or animal | RNA seq from the gonad of Zebrafish | CA2 R1.fq | CA2 R1.fq | control | RNA-Seq | TRANSCRIPTOMIC | RT-PCR | PAIRED | ILLUMINA | Illumina NovaSeq X Plus | SRP545712 | CA2_R1.fq.gz CA2_R2.fq.gz | fastq fastq | 6253035330.0 | 20705415.0 | CA2 R1.fq.gz | SRX26745246 | SRS23234144 | SRA2016483 | Chinese Academy of Fishery Science|Freshwater Fishery Research Center | Chinese Academy of Fishery Science | B | B | biological fallback assumption | illumina | novaseq_era | unknown | other | unknown | bulk | unknown | unknown | China | 2024-11-17 | Juvenile | Juvenile | Gonad | Reproductive System | ||||||||||||||||||||||||||||||||||||||||
| 42181 | 42181 | SRR5482153 | SRX2765568 | SRS2149771 | SRP105243 | PRJNA383986 | Danio rerio Raw sequence reads | PRJNA383986 | Other | The goal of the experiment was to simulate the effect of size selective harvesting and captive rearing on gene expression in a controlled environment using zebrafish as a model species. | Danio rerio: Large harvested line biological replicate 1 F2 offspring originating from wild caught population | LS1 F2 | breed:missing|dev stage:juvenile|sex:female|tissue:liver|death date:2008|BioSampleModel:Model organism or animal | RNAseq of Danio rerio: juvenile female liver RNA of 4 biological replicates from a large harvested selection line F2 individuals | LS1 F2 | LS1 F2 | Total RNA from liver tissue of juvenile zebrafish was extracted according to the Trizol method with TRI reagent Sigma. The liver tissue was homogenized with a TissueLyser II homogenizer Qiagen. DNase treatment was performed to remove any genomic DNA from the samples. RNA was eluted in RNase free water and RNA concentrations and the integrity of RNA samples were determined using an Agilent 2100 Bioanalyzer. Total RNA was processed by using Low Input Library Preparation Kit to prepare the RNA Seq library. | RNA-Seq | TRANSCRIPTOMIC | RT-PCR | SINGLE | ILLUMINA | Illumina HiSeq 2000 | SRP105243 | LS1-F2-10_FCC4CMHACXX_L1_WHZEBagiNCADRAAPEI-205_1.fq.gz LS1-F2-1_FCC4BKHACXX_L7_WHZEBagiNCAARAAPEI-201_1.fq.gz LS1-F2-5_FCC4BKHACXX_L7_WHZEBagiNCABRAAPEI-202_1.fq.gz LS1-F2-8_FCC4BKHACXX_L7_WHZEBagiNCACRAAPEI-203_1.fq.gz | fastq fastq fastq fastq | 12496905120.0 | 255038880.0 | LS1 F2 10 FCC4CMHACXX L1 WHZEBagiNCADRAAPEI 205 1.fq.gz | 0:49 | A:3275093505;C:2981786839;G:2904373605;T:3334855308;N:795863 | 49 | 3275093505 | 2981786839 | 2904373605 | 3334855308 | 795863 | SRX2765568 | SRS2149771 | SRA557107 | University of Turku|Biology | University of Turku | 1 | 0.88577 | 0.04599 | 0.76469 | 0.48703 | 49 | B | usable mapping rate | illumina | hiseq_era | unknown | other | unknown | bulk | unknown | unknown | Finland | 2017-12-04 | Juvenile | Juvenile | Liver | Liver and Biliary System | |||||||||||||||||||||||||||
| 42182 | 42182 | SRR5482152 | SRX2765567 | SRS2149770 | SRP105243 | PRJNA383986 | Danio rerio Raw sequence reads | PRJNA383986 | Other | The goal of the experiment was to simulate the effect of size selective harvesting and captive rearing on gene expression in a controlled environment using zebrafish as a model species. | Danio rerio: Large harvested line biological replicate 2 F2 offspring originating from wild caught population | LS2 F2 | breed:missing|dev stage:juvenile|sex:female|tissue:liver|death date:2008|BioSampleModel:Model organism or animal | RNAseq of Danio rerio: juvenile female liver RNA of 4 biological replicates from a large harvested selection line F2 individuals | LS2 F2 | LS2 F2 | Total RNA from liver tissue of juvenile zebrafish was extracted according to the Trizol method with TRI reagent Sigma. The liver tissue was homogenized with a TissueLyser II homogenizer Qiagen. DNase treatment was performed to remove any genomic DNA from the samples. RNA was eluted in RNase free water and RNA concentrations and the integrity of RNA samples were determined using an Agilent 2100 Bioanalyzer. Total RNA was processed by using Low Input Library Preparation Kit to prepare the RNA Seq library. | RNA-Seq | TRANSCRIPTOMIC | RT-PCR | SINGLE | ILLUMINA | Illumina HiSeq 2000 | SRP105243 | LS2-F2-10_FCC4CMHACXX_L4_WHZEBagiNCALRAAPEI-213_1.fq.gz LS2-F2-11_FCC4CMHACXX_L4_WHZEBagiNCAMRAAPEI-214_1.fq.gz LS2-F2-14_FCC4C2AACXX_L1_WHZEBagiNCANRAAPEI-215_1.fq.gz LS2-F2-9_FCC4CMHACXX_L4_WHZEBagiNCAKRAAPEI-212_1.fq.gz | fastq fastq fastq fastq | 11108547205.0 | 226705045.0 | LS2 F2 9 FCC4CMHACXX L4 WHZEBagiNCAKRAAPEI 212 1.fq.gz | 0:49 | A:2930693084;C:2628276474;G:2564131315;T:2984423047;N:1023285 | 49 | 2930693084 | 2628276474 | 2564131315 | 2984423047 | 1023285 | SRX2765567 | SRS2149770 | SRA557107 | University of Turku|Biology | University of Turku | 1 | 0.90013 | 0.04677 | 0.81251 | 0.44762 | 49 | B | usable mapping rate | illumina | hiseq_era | unknown | other | unknown | bulk | unknown | unknown | Finland | 2017-12-04 | Juvenile | Juvenile | Liver | Liver and Biliary System | |||||||||||||||||||||||||||
| 42183 | 42183 | SRR5482151 | SRX2765565 | SRS2149769 | SRP105243 | PRJNA383986 | Danio rerio Raw sequence reads | PRJNA383986 | Other | The goal of the experiment was to simulate the effect of size selective harvesting and captive rearing on gene expression in a controlled environment using zebrafish as a model species. | Danio rerio: Random harvested line biological replicate 1 F2 offspring originating from wild caught population | RS1 F2 | breed:missing|dev stage:juvenile|sex:female|tissue:liver|death date:2008|BioSampleModel:Model organism or animal | RNAseq of Danio rerio: juvenile female liver RNA of 4 biological replicates from a random harvested selection line F2 individuals | RS1 F2 | RS1 F2 | Total RNA from liver tissue of juvenile zebrafish was extracted according to the Trizol method with TRI reagent Sigma. The liver tissue was homogenized with a TissueLyser II homogenizer Qiagen. DNase treatment was performed to remove any genomic DNA from the samples. RNA was eluted in RNase free water and RNA concentrations and the integrity of RNA samples were determined using an Agilent 2100 Bioanalyzer. Total RNA was processed by using Low Input Library Preparation Kit to prepare the RNA Seq library. | RNA-Seq | TRANSCRIPTOMIC | RT-PCR | SINGLE | ILLUMINA | Illumina HiSeq 2000 | SRP105243 | RS1-F2-14_FCC4C2AACXX_L2_WHZEBagiNCAURAAPEI-223_1.fq.gz RS1-F2-16_FCC4C2AACXX_L2_WHZEBagiNCAVRAAPEI-225_1.fq.gz RS1-F2-17_FCC4C2AACXX_L3_WHZEBagiNCAWRAAPEI-227_1.fq.gz RS1-F2-19_FCC4C2AACXX_L3_WHZEBagiNCAXRAAPEI-201_1.fq.gz | fastq fastq fastq fastq | 12074501894.0 | 246418406.0 | RS1 F2 16 FCC4C2AACXX L2 WHZEBagiNCAVRAAPEI 225 1.fq.gz | 0:49 | A:3199737416;C:2851335695;G:2767557430;T:3254746975;N:1124378 | 49 | 3199737416 | 2851335695 | 2767557430 | 3254746975 | 1124378 | SRX2765565 | SRS2149769 | SRA557107 | University of Turku|Biology | University of Turku | 1 | 0.87838 | 0.05071 | 0.81663 | 0.55417 | 49 | B | usable mapping rate | illumina | hiseq_era | unknown | other | unknown | bulk | unknown | unknown | Finland | 2017-12-04 | Juvenile | Juvenile | Liver | Liver and Biliary System | |||||||||||||||||||||||||||
| 42184 | 42184 | SRR5482150 | SRX2765564 | SRS2149768 | SRP105243 | PRJNA383986 | Danio rerio Raw sequence reads | PRJNA383986 | Other | The goal of the experiment was to simulate the effect of size selective harvesting and captive rearing on gene expression in a controlled environment using zebrafish as a model species. | Danio rerio: Random harvested line biological replicate 2 F2 offspring originating from wild caught population | RS2 F2 | breed:missing|dev stage:juvenile|sex:female|tissue:liver|death date:2008|BioSampleModel:Model organism or animal | RNAseq of Danio rerio: juvenile female liver RNA of 4 biological replicates from a random harvested selection line F2 individuals | RS2 F2 | RS2 F2 | Total RNA from liver tissue of juvenile zebrafish was extracted according to the Trizol method with TRI reagent Sigma. The liver tissue was homogenized with a TissueLyser II homogenizer Qiagen. DNase treatment was performed to remove any genomic DNA from the samples. RNA was eluted in RNase free water and RNA concentrations and the integrity of RNA samples were determined using an Agilent 2100 Bioanalyzer. Total RNA was processed by using Low Input Library Preparation Kit to prepare the RNA Seq library. | RNA-Seq | TRANSCRIPTOMIC | RT-PCR | SINGLE | ILLUMINA | Illumina HiSeq 2000 | SRP105243 | RS2-F2-3_FCC4CMHACXX_L2_WHZEBagiNCBFRAAPEI-210_1.fq.gz RS2-F2-6-2_FCC4CMHACXX_L2_WHZEBagiNCBGRAAPEI-211_1.fq.gz RS2-F2-8_FCC4CMHACXX_L2_WHZEBagiNCBHRABPEI-212_1.fq.gz RS2-F2-9_FCC4CMHACXX_L3_WHZEBagiNCBIRABPEI-213_1.fq.gz | fastq fastq fastq fastq | 11923597525.0 | 243338725.0 | RS2 F2 9 FCC4CMHACXX L3 WHZEBagiNCBIRABPEI 213 1.fq.gz | 0:49 | A:3132507165;C:2819390547;G:2776793842;T:3193588286;N:1317685 | 49 | 3132507165 | 2819390547 | 2776793842 | 3193588286 | 1317685 | SRX2765564 | SRS2149768 | SRA557107 | University of Turku|Biology | University of Turku | 1 | 0.90986 | 0.05269 | 0.814 | 0.36827 | 49 | B | usable mapping rate | illumina | hiseq_era | unknown | other | unknown | bulk | unknown | unknown | Finland | 2017-12-04 | Juvenile | Juvenile | Liver | Liver and Biliary System | |||||||||||||||||||||||||||
| 42185 | 42185 | SRR5482149 | SRX2765563 | SRS2149767 | SRP105243 | PRJNA383986 | Danio rerio Raw sequence reads | PRJNA383986 | Other | The goal of the experiment was to simulate the effect of size selective harvesting and captive rearing on gene expression in a controlled environment using zebrafish as a model species. | Danio rerio: Large harvested line biological replicate 1 F5 offspring originating from wild caught population | LS1 F5 | breed:missing|dev stage:juvenile|sex:female|tissue:liver|death date:2010|BioSampleModel:Model organism or animal | RNAseq of Danio rerio: juvenile female liver RNA of 4 biological replicates from a large harvested selection line F5 individuals | LS1 F5 | LS1 F5 | Total RNA from liver tissue of juvenile zebrafish was extracted according to the Trizol method with TRI reagent Sigma. The liver tissue was homogenized with a TissueLyser II homogenizer Qiagen. DNase treatment was performed to remove any genomic DNA from the samples. RNA was eluted in RNase free water and RNA concentrations and the integrity of RNA samples were determined using an Agilent 2100 Bioanalyzer. Total RNA was processed by using Low Input Library Preparation Kit to prepare the RNA Seq library. | RNA-Seq | TRANSCRIPTOMIC | RT-PCR | SINGLE | ILLUMINA | Illumina HiSeq 2000 | SRP105243 | LS1-15_FCC4C3HACXX_L1_WHZEBagiNBANRAAPEI-215_1.fq.gz LS1-2_FCC4BJ2ACXX_L5_WHZEBagiNBABRAAPEI-202_1.fq.gz LS1-6_FCC4BJ2ACXX_L6_WHZEBagiNBAFRAAPEI-207_1.fq.gz LS1-8_FCC4BJ2ACXX_L8_WHZEBagiNBAJRAAPEI-211_1.fq.gz | fastq fastq fastq fastq | 12776192331.0 | 260738619.0 | LS1 2 FCC4BJ2ACXX L5 WHZEBagiNBABRAAPEI 202 1.fq.gz | 0:49 | A:3371838433;C:3025006624;G:2934135898;T:3444117388;N:1093988 | 49 | 3371838433 | 3025006624 | 2934135898 | 3444117388 | 1093988 | SRX2765563 | SRS2149767 | SRA557107 | University of Turku|Biology | University of Turku | 1 | 0.88482 | 0.04932 | 0.813 | 0.51224 | 49 | B | usable mapping rate | illumina | hiseq_era | unknown | other | unknown | bulk | unknown | unknown | Finland | 2017-12-04 | Juvenile | Juvenile | Liver | Liver and Biliary System | |||||||||||||||||||||||||||
| 42186 | 42186 | SRR5482148 | SRX2765562 | SRS2149766 | SRP105243 | PRJNA383986 | Danio rerio Raw sequence reads | PRJNA383986 | Other | The goal of the experiment was to simulate the effect of size selective harvesting and captive rearing on gene expression in a controlled environment using zebrafish as a model species. | Danio rerio: Large harvested line biological replicate 2 F5 offspring originating from wild caught population | LS2 F5 | breed:missing|dev stage:juvenile|sex:female|tissue:liver|death date:2010|BioSampleModel:Model organism or animal | RNAseq of Danio rerio: juvenile female liver RNA of 4 biological replicates from a large harvested selection line F5 individuals | LS2 F5 | LS2 F5 | Total RNA from liver tissue of juvenile zebrafish was extracted according to the Trizol method with TRI reagent Sigma. The liver tissue was homogenized with a TissueLyser II homogenizer Qiagen. DNase treatment was performed to remove any genomic DNA from the samples. RNA was eluted in RNase free water and RNA concentrations and the integrity of RNA samples were determined using an Agilent 2100 Bioanalyzer. Total RNA was processed by using Low Input Library Preparation Kit to prepare the RNA Seq library. | RNA-Seq | TRANSCRIPTOMIC | RT-PCR | SINGLE | ILLUMINA | Illumina HiSeq 2000 | SRP105243 | LS2-10_FCC4BJ2ACXX_L8_WHZEBagiNBAKRAAPEI-212_1.fq.gz LS2-11_FCC4C3HACXX_L1_WHZEBagiNBAORAAPEI-216_1.fq.gz LS2-1_FCC4BJ2ACXX_L5_WHZEBagiNBACRAAPEI-203_1.fq.gz LS2-7_FCC4BVYACXX_L1_WHZEBagiNBAGRAAPEI-208_1.fq.gz | fastq fastq fastq fastq | 11941143935.0 | 243696815.0 | LS2 10 FCC4BJ2ACXX L8 WHZEBagiNBAKRAAPEI 212 1.fq.gz | 0:49 | A:3165540142;C:2817777586;G:2733117846;T:3223679054;N:1029307 | 49 | 3165540142 | 2817777586 | 2733117846 | 3223679054 | 1029307 | SRX2765562 | SRS2149766 | SRA557107 | University of Turku|Biology | University of Turku | 1 | 0.87119 | 0.04752 | 0.80251 | 0.55444 | 49 | B | usable mapping rate | illumina | hiseq_era | unknown | other | unknown | bulk | unknown | unknown | Finland | 2017-12-04 | Juvenile | Juvenile | Liver | Liver and Biliary System | |||||||||||||||||||||||||||
| 42187 | 42187 | SRR5482147 | SRX2765561 | SRS2149765 | SRP105243 | PRJNA383986 | Danio rerio Raw sequence reads | PRJNA383986 | Other | The goal of the experiment was to simulate the effect of size selective harvesting and captive rearing on gene expression in a controlled environment using zebrafish as a model species. | Danio rerio: Random harvested line biological replicate 1 F5 offspring originating from wild caught population | RS1 F5 | breed:missing|dev stage:juvenile|sex:female|tissue:liver|death date:2010|BioSampleModel:Model organism or animal | RNAseq of Danio rerio: juvenile female liver RNA of 4 biological replicates from a random harvested selection line F5 individuals | RS1 F5 | RS1 F5 | Total RNA from liver tissue of juvenile zebrafish was extracted according to the Trizol method with TRI reagent Sigma. The liver tissue was homogenized with a TissueLyser II homogenizer Qiagen. DNase treatment was performed to remove any genomic DNA from the samples. RNA was eluted in RNase free water and RNA concentrations and the integrity of RNA samples were determined using an Agilent 2100 Bioanalyzer. Total RNA was processed by using Low Input Library Preparation Kit to prepare the RNA Seq library. | RNA-Seq | TRANSCRIPTOMIC | RT-PCR | SINGLE | ILLUMINA | Illumina HiSeq 2000 | SRP105243 | RS1-10_FCC4BJ2ACXX_L8_WHZEBagiNBALRAAPEI-213_1.fq.gz RS1-2_FCC4Y2GACXX_L4_WHZEBagiNCBPRABPEI-221_1.fq.gz RS1-3_FCC4BJ2ACXX_L6_WHZEBagiNBADRAAPEI-205_1.fq.gz RS1-6_FCC4BV7ACXX_L1_WHZEBagiNBAHRAAPEI-209_1.fq.gz | fastq fastq fastq fastq | 12149652753.0 | 247952097.0 | RS1 6 FCC4BV7ACXX L1 WHZEBagiNBAHRAAPEI 209 1.fq.gz | 0:49 | A:3177681868;C:2907323525;G:2822484622;T:3241144133;N:1018605 | 49 | 3177681868 | 2907323525 | 2822484622 | 3241144133 | 1018605 | SRX2765561 | SRS2149765 | SRA557107 | University of Turku|Biology | University of Turku | 1 | 0.90495 | 0.04625 | 0.80028 | 0.56443 | 49 | B | usable mapping rate | illumina | hiseq_era | unknown | other | unknown | bulk | unknown | unknown | Finland | 2017-12-04 | Juvenile | Juvenile | Liver | Liver and Biliary System | |||||||||||||||||||||||||||
| 42188 | 42188 | SRR5482146 | SRX2765560 | SRS2149764 | SRP105243 | PRJNA383986 | Danio rerio Raw sequence reads | PRJNA383986 | Other | The goal of the experiment was to simulate the effect of size selective harvesting and captive rearing on gene expression in a controlled environment using zebrafish as a model species. | Danio rerio: Random harvested line biological replicate 2 F5 offspring originating from wild caught population | RS2 F5 | breed:missing|dev stage:juvenile|sex:female|tissue:liver|death date:2010|BioSampleModel:Model organism or animal | RNAseq of Danio rerio: juvenile female liver RNA of 4 biological replicates from a random harvested selection line F5 individuals | RS2 F5 | RS2 F5 | Total RNA from liver tissue of juvenile zebrafish was extracted according to the Trizol method with TRI reagent Sigma. The liver tissue was homogenized with a TissueLyser II homogenizer Qiagen. DNase treatment was performed to remove any genomic DNA from the samples. RNA was eluted in RNase free water and RNA concentrations and the integrity of RNA samples were determined using an Agilent 2100 Bioanalyzer. Total RNA was processed by using Low Input Library Preparation Kit to prepare the RNA Seq library. | RNA-Seq | TRANSCRIPTOMIC | RT-PCR | SINGLE | ILLUMINA | Illumina HiSeq 2000 | SRP105243 | RS2-10_FCC4BVYACXX_L1_WHZEBagiNBAIRAAPEI-210_1.fq.gz RS2-13_FCC4C3HACXX_L1_WHZEBagiNBAMRAAPEI-214_1.fq.gz RS2-2_FCC4BJ2ACXX_L5_WHZEBagiNBAARAAPEI-201_1.fq.gz RS2-3_FCC4C19ACXX_L5_WHZEBagiNBAERAAPEI-206_1.fq.gz | fastq fastq fastq fastq | 11262796951.0 | 229852999.0 | RS2 13 FCC4C3HACXX L1 WHZEBagiNBAMRAAPEI 214 1.fq.gz | 0:49 | A:2931626627;C:2710523895;G:2627211912;T:2992545637;N:888880 | 49 | 2931626627 | 2710523895 | 2627211912 | 2992545637 | 888880 | SRX2765560 | SRS2149764 | SRA557107 | University of Turku|Biology | University of Turku | 1 | 0.88588 | 0.04266 | 0.8112 | 0.52927 | 49 | B | usable mapping rate | illumina | hiseq_era | unknown | other | unknown | bulk | unknown | unknown | Finland | 2017-12-04 | Juvenile | Juvenile | Liver | Liver and Biliary System | |||||||||||||||||||||||||||
| 42189 | 42189 | SRR5482145 | SRX2765559 | SRS2149763 | SRP105243 | PRJNA383986 | Danio rerio Raw sequence reads | PRJNA383986 | Other | The goal of the experiment was to simulate the effect of size selective harvesting and captive rearing on gene expression in a controlled environment using zebrafish as a model species. | Danio rerio: Large harvested line biological replicate 1 F11 offspring originating from wild caught population | LS1 F11 | breed:missing|dev stage:juvenile|sex:female|tissue:liver|death date:2014|BioSampleModel:Model organism or animal | RNAseq of Danio rerio: juvenile female liver RNA of 4 biological replicates from a large harvested selection line F11 individuals | LS1 F11 | LS1 F11 | Total RNA from liver tissue of juvenile zebrafish was extracted according to the Trizol method with TRI reagent Sigma. The liver tissue was homogenized with a TissueLyser II homogenizer Qiagen. DNase treatment was performed to remove any genomic DNA from the samples. RNA was eluted in RNase free water and RNA concentrations and the integrity of RNA samples were determined using an Agilent 2100 Bioanalyzer. Total RNA was processed by using Low Input Library Preparation Kit to prepare the RNA Seq library. | RNA-Seq | TRANSCRIPTOMIC | RT-PCR | SINGLE | ILLUMINA | Illumina HiSeq 2000 | SRP105243 | LS1-F11B-11_FCC4CMHACXX_L1_WHZEBagiNCAERAAPEI-206_1.fq.gz LS1-F11B-13_FCC4CMHACXX_L1_WHZEBagiNCAFRAAPEI-207_1.fq.gz LS1-F11B-16_FCC4C19ACXX_L1_WHZEBagiNCAGRBAPEI-208_1.fq.gz LS1-F11B-17_FCC4BKHACXX_L8_WHZEBagiNCAHRAAPEI-209_1.fq.gz | fastq fastq fastq fastq | 11326213584.0 | 231147216.0 | LS1 F11B 16 FCC4C19ACXX L1 WHZEBagiNCAGRBAPEI 208 1.fq.gz | 0:49 | A:2982223592;C:2693407623;G:2622876703;T:3026833843;N:871823 | 49 | 2982223592 | 2693407623 | 2622876703 | 3026833843 | 871823 | SRX2765559 | SRS2149763 | SRA557107 | University of Turku|Biology | University of Turku | 1 | 0.90202 | 0.04273 | 0.78273 | 0.51238 | 49 | B | usable mapping rate | illumina | hiseq_era | unknown | other | unknown | bulk | unknown | unknown | Finland | 2017-12-04 | Juvenile | Juvenile | Liver | Liver and Biliary System | |||||||||||||||||||||||||||
| 42190 | 42190 | SRR5482144 | SRX2765558 | SRS2149762 | SRP105243 | PRJNA383986 | Danio rerio Raw sequence reads | PRJNA383986 | Other | The goal of the experiment was to simulate the effect of size selective harvesting and captive rearing on gene expression in a controlled environment using zebrafish as a model species. | Danio rerio: Large harvested line biological replicate 2 F11 offspring originating from wild caught population | LS2 F11 | breed:missing|dev stage:juvenile|sex:female|tissue:liver|death date:2014|BioSampleModel:Model organism or animal | RNAseq of Danio rerio: juvenile female liver RNA of 4 biological replicates from a large harvested selection line F11 individuals | LS2 F11 | LS2 F11 | Total RNA from liver tissue of juvenile zebrafish was extracted according to the Trizol method with TRI reagent Sigma. The liver tissue was homogenized with a TissueLyser II homogenizer Qiagen. DNase treatment was performed to remove any genomic DNA from the samples. RNA was eluted in RNase free water and RNA concentrations and the integrity of RNA samples were determined using an Agilent 2100 Bioanalyzer. Total RNA was processed by using Low Input Library Preparation Kit to prepare the RNA Seq library. | RNA-Seq | TRANSCRIPTOMIC | RT-PCR | SINGLE | ILLUMINA | Illumina HiSeq 2000 | SRP105243 | LS2-F11B-1_FCC4C2AACXX_L1_WHZEBagiNCAORAAPEI-216_1.fq.gz LS2-F11B-2_FCC4C2AACXX_L1_WHZEBagiNCAPRAAPEI-218_1.fq.gz LS2-F11B-5_FCC4CMHACXX_L5_WHZEBagiNCAQRAAPEI-219_1.fq.gz LS2-F11B-7_FCC4CMHACXX_L5_WHZEBagiNCARRAAPEI-220_1.fq.gz | fastq fastq fastq fastq | 12030571875.0 | 245521875.0 | LS2 F11B 7 FCC4CMHACXX L5 WHZEBagiNCARRAAPEI 220 1.fq.gz | 0:49 | A:3149792862;C:2871072895;G:2795019949;T:3213514581;N:1171588 | 49 | 3149792862 | 2871072895 | 2795019949 | 3213514581 | 1171588 | SRX2765558 | SRS2149762 | SRA557107 | University of Turku|Biology | University of Turku | 1 | 0.91893 | 0.036 | 0.77429 | 0.38962 | 49 | B | usable mapping rate | illumina | hiseq_era | unknown | other | unknown | bulk | unknown | unknown | Finland | 2017-12-04 | Juvenile | Juvenile | Liver | Liver and Biliary System | |||||||||||||||||||||||||||
| 42191 | 42191 | SRR5482143 | SRX2765557 | SRS2149760 | SRP105243 | PRJNA383986 | Danio rerio Raw sequence reads | PRJNA383986 | Other | The goal of the experiment was to simulate the effect of size selective harvesting and captive rearing on gene expression in a controlled environment using zebrafish as a model species. | Danio rerio: Random harvested line biological replicate 1 F11 offspring originating from wild caught population | RS1 F11 | breed:missing|dev stage:juvenile|sex:female|tissue:liver|death date:2014|BioSampleModel:Model organism or animal | RNAseq of Danio rerio: juvenile female liver RNA of 4 biological replicates from a random harvested selection line F11 individuals | RS1 F11 | RS1 F11 | Total RNA from liver tissue of juvenile zebrafish was extracted according to the Trizol method with TRI reagent Sigma. The liver tissue was homogenized with a TissueLyser II homogenizer Qiagen. DNase treatment was performed to remove any genomic DNA from the samples. RNA was eluted in RNase free water and RNA concentrations and the integrity of RNA samples were determined using an Agilent 2100 Bioanalyzer. Total RNA was processed by using Low Input Library Preparation Kit to prepare the RNA Seq library. | RNA-Seq | TRANSCRIPTOMIC | RT-PCR | SINGLE | ILLUMINA | Illumina HiSeq 2000 | SRP105243 | RS1-F11B-21_FCC4C2AACXX_L3_WHZEBagiNCAYRAAPEI-202_1.fq.gz RS1-F11B-23_FCC4C2AACXX_L4_WHZEBagiNCAZRAAPEI-203_1.fq.gz RS1-F11B-27_FCC4C2AACXX_L4_WHZEBagiNCBARAAPEI-205_1.fq.gz RS1-F11B-29_FCC4C2AACXX_L4_WHZEBagiNCBBRAAPEI-206_1.fq.gz | fastq fastq fastq fastq | 11947679555.0 | 243830195.0 | RS1 F11B 21 FCC4C2AACXX L3 WHZEBagiNCAYRAAPEI 202 1.fq.gz | 0:49 | A:3159477117;C:2827401119;G:2753495798;T:3206143470;N:1162051 | 49 | 3159477117 | 2827401119 | 2753495798 | 3206143470 | 1162051 | SRX2765557 | SRS2149760 | SRA557107 | University of Turku|Biology | University of Turku | 1 | 0.87877 | 0.04488 | 0.77445 | 0.50852 | 49 | B | usable mapping rate | illumina | hiseq_era | unknown | other | unknown | bulk | unknown | unknown | Finland | 2017-12-04 | Juvenile | Juvenile | Liver | Liver and Biliary System | |||||||||||||||||||||||||||
| 42192 | 42192 | SRR5482142 | SRX2765556 | SRS2149761 | SRP105243 | PRJNA383986 | Danio rerio Raw sequence reads | PRJNA383986 | Other | The goal of the experiment was to simulate the effect of size selective harvesting and captive rearing on gene expression in a controlled environment using zebrafish as a model species. | Danio rerio: Random harvested line biological replicate 2 F11 offspring originating from wild caught population | RS2 F11 | breed:missing|dev stage:juvenile|sex:female|tissue:liver|death date:2014|BioSampleModel:Model organism or animal | RNAseq of Danio rerio: juvenile female liver RNA of 4 biological replicates from a random harvested selection line F11 individuals | RS2 F11 | RS2 F11 | Total RNA from liver tissue of juvenile zebrafish was extracted according to the Trizol method with TRI reagent Sigma. The liver tissue was homogenized with a TissueLyser II homogenizer Qiagen. DNase treatment was performed to remove any genomic DNA from the samples. RNA was eluted in RNase free water and RNA concentrations and the integrity of RNA samples were determined using an Agilent 2100 Bioanalyzer. Total RNA was processed by using Low Input Library Preparation Kit to prepare the RNA Seq library. | RNA-Seq | TRANSCRIPTOMIC | RT-PCR | SINGLE | ILLUMINA | Illumina HiSeq 2000 | SRP105243 | RS2-F11B-34_FCC4CMHACXX_L3_WHZEBagiNCBJRABPEI-214_1.fq.gz RS2-F11B-36_FCC4CMHACXX_L3_WHZEBagiNCBKRABPEI-215_1.fq.gz RS2-F11B-38_FCC4BY1ACXX_L1_WHZEBagiNCBLRABPEI-216_1.fq.gz RS2-F11B-40_FCC4BY1ACXX_L1_WHZEBagiNCBMRABPEI-218_1.fq.gz | fastq fastq fastq fastq | 10619740698.0 | 216729402.0 | RS2 F11B 38 FCC4BY1ACXX L1 WHZEBagiNCBLRABPEI 216 1.fq.gz | 0:49 | A:2799661065;C:2501316593;G:2465115836;T:2852970598;N:676606 | 49 | 2799661065 | 2501316593 | 2465115836 | 2852970598 | 676606 | SRX2765556 | SRS2149761 | SRA557107 | University of Turku|Biology | University of Turku | 1 | 0.8851 | 0.04865 | 0.80407 | 0.54962 | 49 | B | usable mapping rate | illumina | hiseq_era | unknown | other | unknown | bulk | unknown | unknown | Finland | 2017-12-04 | Juvenile | Juvenile | Liver | Liver and Biliary System | |||||||||||||||||||||||||||
| 45031 | 45031 | SRR6466455 | SRX3556412 | SRS2829760 | SRP128941 | PRJNA428924 | Danio rerio breed:TU Transcriptome or Gene expression | PRJNA428924 | Transcriptome Analysis | In order to know about more basic knowledge of none/less gut adhesive strain L. casei BL23 induce on the developmental profile and immunity education in zebrafish | pooled of 15 intestine of juveniles at 35 dpf old. | Control 35 | breed:TU|age:35 dpf|sex:pooled male and female|tissue:intestine|treatment:Control|BioSampleModel:Model organism or animal | RNA seq of zebrafish larvae with or without xxx administration | Ctl 35 | Ctl 35 | Total RNA were isolated from pooled samples of larvae whole body or juvenile s intestines with TRIzol Tian Gen Beijing China following the manufacturer s specifications. The RNA was treated with amplification grade DNase I 1 U/ g RNA; Invitrogen USA. RNA concentration was measured using Qubit RNA Assay Kit in Qubit 2.0 Flurometer Life Technologies CA USA. RNA integrity was assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system Agilent Technologies CA USA. All the RNA RIN values were bigger than 9.5 as analyzed by Bioanalyzer 2100 system. For mRNAseq equal volume and amount 1 g of total RNA from 4 sample pools of each treatment group were mixed as a sequencing sample. | RNA-Seq | TRANSCRIPTOMIC | RT-PCR | PAIRED | ILLUMINA | Illumina HiSeq 2000 | SRP128941 | ZFGCK_1.fq.gz ZFGCK_2.fq.gz | fastq fastq | 4031784000.0 | 16127136.0 | ZFGCK 2.fq.gz | 0:125 1:125 | A:1069662566;C:940723613;G:944143624;T:1077192856;N:61341 | 125 | 125 | 1069662566 | 940723613 | 944143624 | 1077192856 | 61341 | SRX3556412 | SRS2829760 | SRA646164 | Chinese Academy of Agricultural Sciences|Key Laboratory for Feed Biotechnology of the Minis | Chinese Academy of Agricultural Sciences | 2 | 0.93591 | 0.9405 | 0.06779 | 0.06732 | 0.72157 | 0.72372 | 0.53789 | 0.52813 | 125 | 125 | B | B | biological fallback assumption | illumina | hiseq_era | unknown | other | unknown | bulk | unknown | unknown | China | 2018-01-12 | Juvenile | Juvenile | Gut | Digestive System | ||||||||||||||||||||
| 45032 | 45032 | SRR6466456 | SRX3556411 | SRS2829759 | SRP128941 | PRJNA428924 | Danio rerio breed:TU Transcriptome or Gene expression | PRJNA428924 | Transcriptome Analysis | In order to know about more basic knowledge of none/less gut adhesive strain L. casei BL23 induce on the developmental profile and immunity education in zebrafish | pooled of 15 intestine of juveniles at 35 dpf old with BL23 administration | BL23 35 | breed:TU|age:35 dpf|sex:pooled male and female|tissue:intestine|treatment:BL23 administration|BioSampleModel:Model organism or animal | RNA seq of zebrafish larvae with or without xxx administration | BL23 35 | BL23 35 | Total RNA were isolated from pooled samples of larvae whole body or juvenile s intestines with TRIzol Tian Gen Beijing China following the manufacturer s specifications. The RNA was treated with amplification grade DNase I 1 U/ g RNA; Invitrogen USA. RNA concentration was measured using Qubit RNA Assay Kit in Qubit 2.0 Flurometer Life Technologies CA USA. RNA integrity was assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system Agilent Technologies CA USA. All the RNA RIN values were bigger than 9.5 as analyzed by Bioanalyzer 2100 system. For mRNAseq equal volume and amount 1 g of total RNA from 4 sample pools of each treatment group were mixed as a sequencing sample. | RNA-Seq | TRANSCRIPTOMIC | RT-PCR | PAIRED | ILLUMINA | Illumina HiSeq 2000 | SRP128941 | ZFGBL23_1.fq.gz ZFGBL23_2.fq.gz | fastq fastq | 3545702250.0 | 14182809.0 | ZFGBL23 1.fq.gz | 0:125 1:125 | A:931928697;C:835347026;G:838503871;T:939869548;N:53108 | 125 | 125 | 931928697 | 835347026 | 838503871 | 939869548 | 53108 | SRX3556411 | SRS2829759 | SRA646164 | Chinese Academy of Agricultural Sciences|Key Laboratory for Feed Biotechnology of the Minis | Chinese Academy of Agricultural Sciences | 2 | 0.94166 | 0.94611 | 0.05678 | 0.05657 | 0.71652 | 0.71894 | 0.51987 | 0.53609 | 125 | 125 | B | B | biological fallback assumption | illumina | hiseq_era | unknown | other | unknown | bulk | unknown | unknown | China | 2018-01-12 | Juvenile | Juvenile | Gut | Digestive System | ||||||||||||||||||||
| 60599 | 60599 | SRR12432918 | SRX8928709 | SRS7184991 | SRP277140 | PRJNA656674 | EGFR mutant Danio rerio ovary raw sequence reads | PRJNA656674 | Whole Genome Sequencing | The ovaries of 45 dpf zebrafish were separated. Transcriptome analysis between EGFR mutant and wildtype zebrafish was conducted in this project. | The zebrafish ovaries isolated from 45 dpf female were analyzed. | Ovary of 45 dpf Danio rerio | zebrafish | strain:AB|age:45 dpf|dev stage:puberty|sex:female|tissue:ovary|BioSampleModel:Model organism or animal | WT2 | 5 | 5 | common method | RNA-Seq | TRANSCRIPTOMIC | RT-PCR | PAIRED | ILLUMINA | Illumina iSeq 100 | SRP277140 | egfraWT2_1.fq.gz egfraWT2_2.fq.gz | fastq fastq | 4505046300.0 | 15016821.0 | egfraWT2 1.fq.gz | 0:150 1:150 | A:1178064156;C:1082694104;G:1073742824;T:1169756132;N:789084 | 150 | 150 | 1178064156 | 1082694104 | 1073742824 | 1169756132 | 789084 | SRX8928709 | SRS7184991 | SRA1111906 | Faculty of Health Sciences, University of Macau|Centre of Reproduction, Development and Aging | Faculty of Health Sciences, University of Macau | 2 | 0.89561 | 0.8985 | 0.02356 | 0.02385 | 0.74383 | 0.74953 | 0.47901 | 0.47332 | 150 | 150 | B | B | biological fallback assumption | illumina | miseq | unknown | other | unknown | bulk | unknown | unknown | China | 2020-08-12 | Juvenile | Juvenile | Gonad | Reproductive System | |||||||||||||||||||
| 60600 | 60600 | SRR12432919 | SRX8928708 | SRS7184991 | SRP277140 | PRJNA656674 | EGFR mutant Danio rerio ovary raw sequence reads | PRJNA656674 | Whole Genome Sequencing | The ovaries of 45 dpf zebrafish were separated. Transcriptome analysis between EGFR mutant and wildtype zebrafish was conducted in this project. | The zebrafish ovaries isolated from 45 dpf female were analyzed. | Ovary of 45 dpf Danio rerio | zebrafish | strain:AB|age:45 dpf|dev stage:puberty|sex:female|tissue:ovary|BioSampleModel:Model organism or animal | WT1 | 4 | 4 | common method | RNA-Seq | TRANSCRIPTOMIC | RT-PCR | PAIRED | ILLUMINA | Illumina iSeq 100 | SRP277140 | egfraWT1_2.fq.gz egfraWT1_1.fq.gz | fastq fastq | 4315818300.0 | 14386061.0 | egfraWT1 1.fq.gz | 0:150 1:150 | A:1130385340;C:1035711280;G:1025882601;T:1123083064;N:756015 | 150 | 150 | 1130385340 | 1035711280 | 1025882601 | 1123083064 | 756015 | SRX8928708 | SRS7184991 | SRA1111906 | Faculty of Health Sciences, University of Macau|Centre of Reproduction, Development and Aging | Faculty of Health Sciences, University of Macau | 2 | 0.90084 | 0.90364 | 0.02361 | 0.0238 | 0.73941 | 0.74452 | 0.4717 | 0.47805 | 150 | 150 | B | B | biological fallback assumption | illumina | miseq | unknown | other | unknown | bulk | unknown | unknown | China | 2020-08-12 | Juvenile | Juvenile | Gonad | Reproductive System | |||||||||||||||||||
| 60601 | 60601 | SRR12432920 | SRX8928707 | SRS7184991 | SRP277140 | PRJNA656674 | EGFR mutant Danio rerio ovary raw sequence reads | PRJNA656674 | Whole Genome Sequencing | The ovaries of 45 dpf zebrafish were separated. Transcriptome analysis between EGFR mutant and wildtype zebrafish was conducted in this project. | The zebrafish ovaries isolated from 45 dpf female were analyzed. | Ovary of 45 dpf Danio rerio | zebrafish | strain:AB|age:45 dpf|dev stage:puberty|sex:female|tissue:ovary|BioSampleModel:Model organism or animal | KO3 | 3 | 3 | common method | RNA-Seq | TRANSCRIPTOMIC | RT-PCR | PAIRED | ILLUMINA | Illumina iSeq 100 | SRP277140 | egfraKO3_2.fq.gz egfraKO3_1.fq.gz | fastq fastq | 8905879200.0 | 29686264.0 | egfraKO3 1.fq.gz | 0:150 1:150 | A:2348479134;C:2123503765;G:2100165882;T:2332167190;N:1563229 | 150 | 150 | 2348479134 | 2123503765 | 2100165882 | 2332167190 | 1563229 | SRX8928707 | SRS7184991 | SRA1111906 | Faculty of Health Sciences, University of Macau|Centre of Reproduction, Development and Aging | Faculty of Health Sciences, University of Macau | 2 | 0.89938 | 0.90015 | 0.02459 | 0.02443 | 0.73959 | 0.74619 | 0.47427 | 0.48032 | 150 | 150 | B | B | biological fallback assumption | illumina | miseq | unknown | other | unknown | bulk | unknown | unknown | China | 2020-08-12 | Juvenile | Juvenile | Gonad | Reproductive System | |||||||||||||||||||
| 60602 | 60602 | SRR12432921 | SRX8928706 | SRS7184991 | SRP277140 | PRJNA656674 | EGFR mutant Danio rerio ovary raw sequence reads | PRJNA656674 | Whole Genome Sequencing | The ovaries of 45 dpf zebrafish were separated. Transcriptome analysis between EGFR mutant and wildtype zebrafish was conducted in this project. | The zebrafish ovaries isolated from 45 dpf female were analyzed. | Ovary of 45 dpf Danio rerio | zebrafish | strain:AB|age:45 dpf|dev stage:puberty|sex:female|tissue:ovary|BioSampleModel:Model organism or animal | KO2 | 2 | 2 | common method | RNA-Seq | TRANSCRIPTOMIC | RT-PCR | PAIRED | ILLUMINA | Illumina iSeq 100 | SRP277140 | egfraKO2_1.fq.gz egfraKO2_2.fq.gz | fastq fastq | 8150314500.0 | 27167715.0 | egfraKO2 1.fq.gz | 0:150 1:150 | A:2142556905;C:1947068596;G:1929502017;T:2129761480;N:1425502 | 150 | 150 | 2142556905 | 1947068596 | 1929502017 | 2129761480 | 1425502 | SRX8928706 | SRS7184991 | SRA1111906 | Faculty of Health Sciences, University of Macau|Centre of Reproduction, Development and Aging | Faculty of Health Sciences, University of Macau | 2 | 0.89813 | 0.89911 | 0.0236 | 0.0243 | 0.74562 | 0.75097 | 0.47766 | 0.48014 | 150 | 150 | B | B | biological fallback assumption | illumina | miseq | unknown | other | unknown | bulk | unknown | unknown | China | 2020-08-12 | Juvenile | Juvenile | Gonad | Reproductive System | |||||||||||||||||||
| 60603 | 60603 | SRR12432922 | SRX8928705 | SRS7184991 | SRP277140 | PRJNA656674 | EGFR mutant Danio rerio ovary raw sequence reads | PRJNA656674 | Whole Genome Sequencing | The ovaries of 45 dpf zebrafish were separated. Transcriptome analysis between EGFR mutant and wildtype zebrafish was conducted in this project. | The zebrafish ovaries isolated from 45 dpf female were analyzed. | Ovary of 45 dpf Danio rerio | zebrafish | strain:AB|age:45 dpf|dev stage:puberty|sex:female|tissue:ovary|BioSampleModel:Model organism or animal | KO1 | 1 | 1 | common method | RNA-Seq | TRANSCRIPTOMIC | RT-PCR | PAIRED | ILLUMINA | Illumina iSeq 100 | SRP277140 | egfraKO1_1.fq.gz egfraKO1_2.fq.gz | fastq fastq | 8737229100.0 | 29124097.0 | egfraKO1 1.fq.gz | 0:150 1:150 | A:2303932995;C:2082372983;G:2062052221;T:2287339032;N:1531869 | 150 | 150 | 2303932995 | 2082372983 | 2062052221 | 2287339032 | 1531869 | SRX8928705 | SRS7184991 | SRA1111906 | Faculty of Health Sciences, University of Macau|Centre of Reproduction, Development and Aging | Faculty of Health Sciences, University of Macau | 2 | 0.89994 | 0.9013 | 0.02483 | 0.02509 | 0.74497 | 0.7511 | 0.47316 | 0.4729 | 150 | 150 | B | B | biological fallback assumption | illumina | miseq | unknown | other | unknown | bulk | unknown | unknown | China | 2020-08-12 | Juvenile | Juvenile | Gonad | Reproductive System | |||||||||||||||||||
| 60604 | 60604 | SRR12432923 | SRX8928704 | SRS7184991 | SRP277140 | PRJNA656674 | EGFR mutant Danio rerio ovary raw sequence reads | PRJNA656674 | Whole Genome Sequencing | The ovaries of 45 dpf zebrafish were separated. Transcriptome analysis between EGFR mutant and wildtype zebrafish was conducted in this project. | The zebrafish ovaries isolated from 45 dpf female were analyzed. | Ovary of 45 dpf Danio rerio | zebrafish | strain:AB|age:45 dpf|dev stage:puberty|sex:female|tissue:ovary|BioSampleModel:Model organism or animal | WT3 | 6 | 6 | common method | RNA-Seq | TRANSCRIPTOMIC | RT-PCR | PAIRED | ILLUMINA | Illumina iSeq 100 | SRP277140 | egfraWT3_1.fq.gz egfraWT3_2.fq.gz | fastq fastq | 4200074700.0 | 14000249.0 | egfraWT3 1.fq.gz | 0:150 1:150 | A:1106327217;C:1001998393;G:992359377;T:1098651320;N:738393 | 150 | 150 | 1106327217 | 1001998393 | 992359377 | 1098651320 | 738393 | SRX8928704 | SRS7184991 | SRA1111906 | Faculty of Health Sciences, University of Macau|Centre of Reproduction, Development and Aging | Faculty of Health Sciences, University of Macau | 2 | 0.89695 | 0.90079 | 0.02495 | 0.0254 | 0.74304 | 0.74795 | 0.47396 | 0.48144 | 150 | 150 | B | B | biological fallback assumption | illumina | miseq | unknown | other | unknown | bulk | unknown | unknown | China | 2020-08-12 | Juvenile | Juvenile | Gonad | Reproductive System | |||||||||||||||||||
| 66710 | 66710 | SRR16378894 | SRX12656337 | SRS10609555 | SRP341786 | PRJNA772011 | Spatial transcriptome of zebrafish trunk section | PRJNA772011 | Other | Spatial transcriptome sequencing data from sections of adult and larvae zebrafish trunk. Aiming to delineate the distribution of fast and slow muscle fibers in zebrafish by transcriptomic patterns and the regulation of muscle growth. | zebrafish larvae | strain:Riken wide type|age:1 month|sex:not applicable|tissue:muscle|birth location:Tokyo Japan|collection date:2020 08 20|BioSampleModel:Model organism or animal | RNA seq of Danio rerio: larvae muscle | zebrafish larva | zebrafish larva | Visium spatial transcriptomics | RNA-Seq | TRANSCRIPTOMIC | RT-PCR | PAIRED | ILLUMINA | HiSeq X Ten | SRP341786 | zebra_young_S1_L004_R1_001.fastq zebra_young_S1_L004_R2_001.fastq | fastq fastq | 2755287020.0 | 23349890.0 | zebra young S1 L004 R1 001.fastq | 0:28 1:90 | A:775062912;C:621131964;G:654720744;T:704362634;N:8766 | 28 | 90 | 775062912 | 621131964 | 654720744 | 704362634 | 8766 | SRX12656337 | SRS10609555 | SRA1312274 | The University of Tokyo|Graduate School of Agriculture and Life Science | The University of Tokyo | 2 | 0.00167 | 0.91629 | 0.0005 | 0.02744 | 0.99707 | 0.85989 | 0.57777 | 0.51307 | 28 | 90 | T | B | sc-like readlen | illumina | hiseq_era | unknown | other | unknown | bulk | unknown | unknown | Japan | 2021-10-17 | Juvenile | Juvenile | Muscle | Muscular System | |||||||||||||||||||||
| 66831 | 66831 | SRR16674680 | SRX12875367 | SRS10818487 | SRP343978 | PRJNA776712 | RNA seq analysis of nde1 mutant zebrafish | PRJNA776712 | Other | We perform RNA sequencing to detect defferential expressed genes of nde1 deficiency mutant zebrafish brains compared to wild type. | brain nde1KO | strain:nde1KO|dev stage:2mpf|sex:male|tissue:brain|BioSampleModel:Model organism or animal | RNA seq of nde1 ko Danio rerio brain | B8 | B8 | RNA seq transcriptome librariy was prepared following TruSeqTM RNA sample preparation Kit from Illumina San Diego CA using 5g of total RNA. Shortly messenger RNA was isolated according to polyA selection method by oligodT beads and then fragmented by fragmentation buffer firstly. Secondly double stranded cDNA was synthesized using a SuperScript double stranded cDNA synthesis kit Invitrogen CA with random hexamer primers Illumina. Then the synthesized cDNA was subjected to end repair phosphorylation and A base addition according to Illuminas library construction protocol. Libraries were size selected for cDNA target fragments of 200300 bp on 2% Low Range Ultra Agarose followed by PCR amplified using Phusion DNA polymerase NEB for 15 PCR cycles. post quantified by TBS380 paired end RNA seq sequencing library was sequenced with the Illumina HiSeq 4000 2 150bp read length. | RNA-Seq | TRANSCRIPTOMIC | RT-PCR | PAIRED | ILLUMINA | Illumina HiSeq 4000 | SRP343978 | B8.R1.fastq.gz B8.R2.fastq.gz | fastq fastq | 6730442668.0 | 22286234.0 | B8.R1.fastq.gz | 0:151 1:151 | A:1861252434;C:1503025278;G:1520846977;T:1845259260;N:58719 | 151 | 151 | 1861252434 | 1503025278 | 1520846977 | 1845259260 | 58719 | SRX12875367 | SRS10818487 | Children's Hospital of Fudan University | 2 | 0.94017 | 0.93241 | 0.13042 | 0.12914 | 0.68958 | 0.69132 | 0.48508 | 0.48284 | 151 | 151 | B | B | biological fallback assumption | illumina | hiseq_era | unknown | poly_a | trueseq | bulk | unknown | unknown | China | 2021-11-01 | Juvenile | Juvenile | Brain | Nervous System | |||||||||||||||||||||||
| 66832 | 66832 | SRR16674681 | SRX12875366 | SRS10818487 | SRP343978 | PRJNA776712 | RNA seq analysis of nde1 mutant zebrafish | PRJNA776712 | Other | We perform RNA sequencing to detect defferential expressed genes of nde1 deficiency mutant zebrafish brains compared to wild type. | brain nde1KO | strain:nde1KO|dev stage:2mpf|sex:male|tissue:brain|BioSampleModel:Model organism or animal | RNA seq of nde1 ko Danio rerio brain | B5 | B5 | RNA seq transcriptome librariy was prepared following TruSeqTM RNA sample preparation Kit from Illumina San Diego CA using 5g of total RNA. Shortly messenger RNA was isolated according to polyA selection method by oligodT beads and then fragmented by fragmentation buffer firstly. Secondly double stranded cDNA was synthesized using a SuperScript double stranded cDNA synthesis kit Invitrogen CA with random hexamer primers Illumina. Then the synthesized cDNA was subjected to end repair phosphorylation and A base addition according to Illuminas library construction protocol. Libraries were size selected for cDNA target fragments of 200300 bp on 2% Low Range Ultra Agarose followed by PCR amplified using Phusion DNA polymerase NEB for 15 PCR cycles. post quantified by TBS380 paired end RNA seq sequencing library was sequenced with the Illumina HiSeq 4000 2 150bp read length. | RNA-Seq | TRANSCRIPTOMIC | RT-PCR | PAIRED | ILLUMINA | Illumina HiSeq 4000 | SRP343978 | B5.R1.fastq.gz B5.R2.fastq.gz | fastq fastq | 7110747040.0 | 23545520.0 | B5.R1.fastq.gz | 0:151 1:151 | A:1977355863;C:1580608625;G:1601936422;T:1950785340;N:60790 | 151 | 151 | 1977355863 | 1580608625 | 1601936422 | 1950785340 | 60790 | SRX12875366 | SRS10818487 | Children's Hospital of Fudan University | 2 | 0.93839 | 0.92891 | 0.1351 | 0.13386 | 0.69175 | 0.69424 | 0.49143 | 0.49249 | 151 | 151 | B | B | biological fallback assumption | illumina | hiseq_era | unknown | poly_a | trueseq | bulk | unknown | unknown | China | 2021-11-01 | Juvenile | Juvenile | Brain | Nervous System | |||||||||||||||||||||||
| 66833 | 66833 | SRR16674682 | SRX12875365 | SRS10818487 | SRP343978 | PRJNA776712 | RNA seq analysis of nde1 mutant zebrafish | PRJNA776712 | Other | We perform RNA sequencing to detect defferential expressed genes of nde1 deficiency mutant zebrafish brains compared to wild type. | brain nde1KO | strain:nde1KO|dev stage:2mpf|sex:male|tissue:brain|BioSampleModel:Model organism or animal | RNA seq of nde1 ko Danio rerio brain | B2 | B2 | RNA seq transcriptome librariy was prepared following TruSeqTM RNA sample preparation Kit from Illumina San Diego CA using 5g of total RNA. Shortly messenger RNA was isolated according to polyA selection method by oligodT beads and then fragmented by fragmentation buffer firstly. Secondly double stranded cDNA was synthesized using a SuperScript double stranded cDNA synthesis kit Invitrogen CA with random hexamer primers Illumina. Then the synthesized cDNA was subjected to end repair phosphorylation and A base addition according to Illuminas library construction protocol. Libraries were size selected for cDNA target fragments of 200300 bp on 2% Low Range Ultra Agarose followed by PCR amplified using Phusion DNA polymerase NEB for 15 PCR cycles. post quantified by TBS380 paired end RNA seq sequencing library was sequenced with the Illumina HiSeq 4000 2 150bp read length. | RNA-Seq | TRANSCRIPTOMIC | RT-PCR | PAIRED | ILLUMINA | Illumina HiSeq 4000 | SRP343978 | B2.R1.fastq.gz B2.R2.fastq.gz | fastq fastq | 8547028538.0 | 28301419.0 | B2.R1.fastq.gz | 0:151 1:151 | A:2378598350;C:1891330158;G:1921569124;T:2355455648;N:75258 | 151 | 151 | 2378598350 | 1891330158 | 1921569124 | 2355455648 | 75258 | SRX12875365 | SRS10818487 | Children's Hospital of Fudan University | 2 | 0.94029 | 0.92534 | 0.13616 | 0.13373 | 0.68949 | 0.69219 | 0.47948 | 0.48398 | 151 | 151 | B | B | biological fallback assumption | illumina | hiseq_era | unknown | poly_a | trueseq | bulk | unknown | unknown | China | 2021-11-01 | Juvenile | Juvenile | Brain | Nervous System | |||||||||||||||||||||||
| 66834 | 66834 | SRR16674683 | SRX12875364 | SRS10818488 | SRP343978 | PRJNA776712 | RNA seq analysis of nde1 mutant zebrafish | PRJNA776712 | Other | We perform RNA sequencing to detect defferential expressed genes of nde1 deficiency mutant zebrafish brains compared to wild type. | brain WT | strain:TU|dev stage:2mpf|sex:male|tissue:brain|BioSampleModel:Model organism or animal | RNA seq of Danio rerio brain | B7 | B7 | RNA seq transcriptome librariy was prepared following TruSeqTM RNA sample preparation Kit from Illumina San Diego CA using 5g of total RNA. Shortly messenger RNA was isolated according to polyA selection method by oligodT beads and then fragmented by fragmentation buffer firstly. Secondly double stranded cDNA was synthesized using a SuperScript double stranded cDNA synthesis kit Invitrogen CA with random hexamer primers Illumina. Then the synthesized cDNA was subjected to end repair phosphorylation and A base addition according to Illuminas library construction protocol. Libraries were size selected for cDNA target fragments of 200300 bp on 2% Low Range Ultra Agarose followed by PCR amplified using Phusion DNA polymerase NEB for 15 PCR cycles. post quantified by TBS380 paired end RNA seq sequencing library was sequenced with the Illumina HiSeq 4000 2 150bp read length. | RNA-Seq | TRANSCRIPTOMIC | RT-PCR | PAIRED | ILLUMINA | Illumina HiSeq 4000 | SRP343978 | B7.R1.fastq.gz B7.R2.fastq.gz | fastq fastq | 6685388496.0 | 22137048.0 | B7.R1.fastq.gz | 0:151 1:151 | A:1860264075;C:1483831512;G:1503216220;T:1838014556;N:62133 | 151 | 151 | 1860264075 | 1483831512 | 1503216220 | 1838014556 | 62133 | SRX12875364 | SRS10818488 | Children's Hospital of Fudan University | 2 | 0.94254 | 0.93341 | 0.13834 | 0.13689 | 0.69355 | 0.69755 | 0.49201 | 0.49217 | 151 | 151 | B | B | biological fallback assumption | illumina | hiseq_era | unknown | poly_a | trueseq | bulk | unknown | unknown | China | 2021-11-01 | Juvenile | Juvenile | Brain | Nervous System | |||||||||||||||||||||||
| 66835 | 66835 | SRR16674684 | SRX12875363 | SRS10818488 | SRP343978 | PRJNA776712 | RNA seq analysis of nde1 mutant zebrafish | PRJNA776712 | Other | We perform RNA sequencing to detect defferential expressed genes of nde1 deficiency mutant zebrafish brains compared to wild type. | brain WT | strain:TU|dev stage:2mpf|sex:male|tissue:brain|BioSampleModel:Model organism or animal | RNA seq of Danio rerio brain | B4 | B4 | RNA seq transcriptome librariy was prepared following TruSeqTM RNA sample preparation Kit from Illumina San Diego CA using 5g of total RNA. Shortly messenger RNA was isolated according to polyA selection method by oligodT beads and then fragmented by fragmentation buffer firstly. Secondly double stranded cDNA was synthesized using a SuperScript double stranded cDNA synthesis kit Invitrogen CA with random hexamer primers Illumina. Then the synthesized cDNA was subjected to end repair phosphorylation and A base addition according to Illuminas library construction protocol. Libraries were size selected for cDNA target fragments of 200300 bp on 2% Low Range Ultra Agarose followed by PCR amplified using Phusion DNA polymerase NEB for 15 PCR cycles. post quantified by TBS380 paired end RNA seq sequencing library was sequenced with the Illumina HiSeq 4000 2 150bp read length. | RNA-Seq | TRANSCRIPTOMIC | RT-PCR | PAIRED | ILLUMINA | Illumina HiSeq 4000 | SRP343978 | B4.R1.fastq.gz B4.R2.fastq.gz | fastq fastq | 7546711220.0 | 24989110.0 | B4.R1.fastq.gz | 0:151 1:151 | A:2099092660;C:1670910951;G:1699960602;T:2076680479;N:66528 | 151 | 151 | 2099092660 | 1670910951 | 1699960602 | 2076680479 | 66528 | SRX12875363 | SRS10818488 | Children's Hospital of Fudan University | 2 | 0.94254 | 0.92745 | 0.13347 | 0.13114 | 0.69225 | 0.69424 | 0.48413 | 0.49124 | 151 | 151 | B | B | biological fallback assumption | illumina | hiseq_era | unknown | poly_a | trueseq | bulk | unknown | unknown | China | 2021-11-01 | Juvenile | Juvenile | Brain | Nervous System | |||||||||||||||||||||||
| 66836 | 66836 | SRR16674685 | SRX12875362 | SRS10818488 | SRP343978 | PRJNA776712 | RNA seq analysis of nde1 mutant zebrafish | PRJNA776712 | Other | We perform RNA sequencing to detect defferential expressed genes of nde1 deficiency mutant zebrafish brains compared to wild type. | brain WT | strain:TU|dev stage:2mpf|sex:male|tissue:brain|BioSampleModel:Model organism or animal | RNA seq of Danio rerio brain | B1 | B1 | RNA seq transcriptome librariy was prepared following TruSeqTM RNA sample preparation Kit from Illumina San Diego CA using 5g of total RNA. Shortly messenger RNA was isolated according to polyA selection method by oligodT beads and then fragmented by fragmentation buffer firstly. Secondly double stranded cDNA was synthesized using a SuperScript double stranded cDNA synthesis kit Invitrogen CA with random hexamer primers Illumina. Then the synthesized cDNA was subjected to end repair phosphorylation and A base addition according to Illuminas library construction protocol. Libraries were size selected for cDNA target fragments of 200300 bp on 2% Low Range Ultra Agarose followed by PCR amplified using Phusion DNA polymerase NEB for 15 PCR cycles. post quantified by TBS380 paired end RNA seq sequencing library was sequenced with the Illumina HiSeq 4000 2 150bp read length. | RNA-Seq | TRANSCRIPTOMIC | RT-PCR | PAIRED | ILLUMINA | Illumina HiSeq 4000 | SRP343978 | B1.R1.fastq.gz B1.R2.fastq.gz | fastq fastq | 6758464342.0 | 22379021.0 | B1.R1.fastq.gz | 0:151 1:151 | A:1881676275;C:1495234309;G:1519420774;T:1862074223;N:58761 | 151 | 151 | 1881676275 | 1495234309 | 1519420774 | 1862074223 | 58761 | SRX12875362 | SRS10818488 | Children's Hospital of Fudan University | 2 | 0.94263 | 0.92421 | 0.13676 | 0.13324 | 0.69505 | 0.697 | 0.48389 | 0.48239 | 151 | 151 | B | B | biological fallback assumption | illumina | hiseq_era | unknown | poly_a | trueseq | bulk | unknown | unknown | China | 2021-11-02 | Juvenile | Juvenile | Brain | Nervous System | |||||||||||||||||||||||
| 68554 | 68554 | SRR18010263 | SRX14164590 | SRS11988750 | SRP359660 | PRJNA806676 | Transcriptome analysis of nomo1 homologous deficiency zebrafish | PRJNA806676 | Other | This analysis revealed transcriptome level changes of brain of nomo1 homologous deficiency zebrafish. | nomo1 | strain:nomo / |dev stage:2mpf|sex:male|tissue:brain|BioSampleModel:Model organism or animal | RNA seq of Danio rario: nomo1 | nomo | nomo | RNA seq transcriptome librariy was prepared following TruSeqTM RNA sample preparation Kit from Illumina San Diego CA using 5g of total RNA. Shortly messenger RNA was isolated according to polyA selection method by oligodT beads and then fragmented by fragmentation buffer firstly. Secondly double stranded cDNA was synthesized using a SuperScript double stranded cDNA synthesis kit Invitrogen CA with random hexamer primers Illumina. Then the synthesized cDNA was subjected to end repair phosphorylation and A base addition according to Illuminas library construction protocol. Libraries were size selected for cDNA target fragments of 200300 bp on 2% Low Range Ultra Agarose followed by PCR amplified using Phusion DNA polymerase NEB for 15 PCR cycles. post quantified by TBS380 paired end RNA seq sequencing library was sequenced with the Illumina HiSeq 4000 2 150bp read length. | RNA-Seq | TRANSCRIPTOMIC | RT-PCR | PAIRED | ILLUMINA | Illumina HiSeq 4000 | SRP359660 | NOMO_1.fq.gz NOMO_2.fq.gz | fastq fastq | 7423945800.0 | 24746486.0 | NOMO 1.fq.gz | 0:150 1:150 | A:2079187833;C:1643658691;G:1652867282;T:2048199003;N:32991 | 150 | 150 | 2079187833 | 1643658691 | 1652867282 | 2048199003 | 32991 | SRX14164590 | SRS11988750 | Children's Hospital of Fudan University | 2 | 0.93796 | 0.93625 | 0.16379 | 0.1632 | 0.69643 | 0.69842 | 0.48865 | 0.48976 | 150 | 150 | B | B | biological fallback assumption | illumina | hiseq_era | unknown | poly_a | trueseq | bulk | unknown | unknown | China | 2022-02-13 | Juvenile | Juvenile | Brain | Nervous System | |||||||||||||||||||||||
| 68555 | 68555 | SRR18010264 | SRX14164589 | SRS11988749 | SRP359660 | PRJNA806676 | Transcriptome analysis of nomo1 homologous deficiency zebrafish | PRJNA806676 | Other | This analysis revealed transcriptome level changes of brain of nomo1 homologous deficiency zebrafish. | WT | strain:tu|dev stage:2mpf|sex:male|tissue:brain|BioSampleModel:Model organism or animal | RNA seq of Danio rario: TU | TU | TU | RNA seq transcriptome librariy was prepared following TruSeqTM RNA sample preparation Kit from Illumina San Diego CA using 5g of total RNA. Shortly messenger RNA was isolated according to polyA selection method by oligodT beads and then fragmented by fragmentation buffer firstly. Secondly double stranded cDNA was synthesized using a SuperScript double stranded cDNA synthesis kit Invitrogen CA with random hexamer primers Illumina. Then the synthesized cDNA was subjected to end repair phosphorylation and A base addition according to Illuminas library construction protocol. Libraries were size selected for cDNA target fragments of 200300 bp on 2% Low Range Ultra Agarose followed by PCR amplified using Phusion DNA polymerase NEB for 15 PCR cycles. post quantified by TBS380 paired end RNA seq sequencing library was sequenced with the Illumina HiSeq 4000 2 150bp read length. | RNA-Seq | TRANSCRIPTOMIC | RT-PCR | PAIRED | ILLUMINA | Illumina HiSeq 4000 | SRP359660 | TU_1.fq.gz TU_2.fq.gz | fastq fastq | 7981027200.0 | 26603424.0 | TU 1.fq.gz | 0:150 1:150 | A:2246332345;C:1756444706;G:1767372264;T:2210842856;N:35029 | 150 | 150 | 2246332345 | 1756444706 | 1767372264 | 2210842856 | 35029 | SRX14164589 | SRS11988749 | Children's Hospital of Fudan University | 2 | 0.93375 | 0.93291 | 0.17081 | 0.17028 | 0.69755 | 0.69875 | 0.48942 | 0.49405 | 150 | 150 | B | B | biological fallback assumption | illumina | hiseq_era | unknown | poly_a | trueseq | bulk | unknown | unknown | China | 2022-02-13 | Juvenile | Juvenile | Brain | Nervous System |
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CREATE TABLE run_metadata("run.accession" VARCHAR, "experiment.accession" VARCHAR, "sample.accession" VARCHAR, "study.accession" VARCHAR, bioproject VARCHAR, "study.title" VARCHAR, "study.alias" VARCHAR, "study.type" VARCHAR, "study.abstract" VARCHAR, "study.attributes" VARCHAR, "study.PMIDs" VARCHAR, "sample.description" VARCHAR, "sample.title" VARCHAR, "sample.alias" VARCHAR, "sample.centername" VARCHAR, "sample.attributes" VARCHAR, "GEOsample.title" VARCHAR, "GEOsample.dataprocessing" VARCHAR, "GEOsample.source" VARCHAR, "GEOsample.treatmentprotocol" VARCHAR, "GEOsample.extractprotocol" VARCHAR, "GEOsample.growthprotocol" VARCHAR, "GEOsample.characteristics" VARCHAR, "GEOsample.accession" VARCHAR, "experiment.title" VARCHAR, "experiment.alias" VARCHAR, "experiment.library_name" VARCHAR, "experiment.design_description" VARCHAR, "experiment.library_construction_protocol" VARCHAR, "experiment.attributes" VARCHAR, "experiment.library_strategy" VARCHAR, "experiment.library_source" VARCHAR, "experiment.library_selection" VARCHAR, "experiment.library_layout" VARCHAR, "experiment.platform" VARCHAR, "experiment.instrument_model" VARCHAR, "experiment.spot_descriptor" VARCHAR, "experiment.study_ref" VARCHAR, "run.title" VARCHAR, "run.attributes" VARCHAR, "run.filename" VARCHAR, "run.semantic_name" VARCHAR, "run.total_bases" DOUBLE, "run.total_spots" DOUBLE, "run.alias" VARCHAR, "run.read_lengths" VARCHAR, "run.base_counts" VARCHAR, "run.r1_length" BIGINT, "run.r2_length" BIGINT, "run.r3_length" BIGINT, "run.r4_length" BIGINT, "run.Acount" BIGINT, "run.Ccount" BIGINT, "run.Gcount" BIGINT, "run.Tcount" BIGINT, "run.Ncount" BIGINT, "run.experiment" VARCHAR, "run.pool_member" VARCHAR, "submission.accession" VARCHAR, "submission.srasource" VARCHAR, "submission.bioprojectsource" VARCHAR, "seqdetective.n_mates" BIGINT, "seqdetective.mapping_rate.mate1" DOUBLE, "seqdetective.mapping_rate.mate2" DOUBLE, "seqdetective.nofeature_rate.mate1" DOUBLE, "seqdetective.nofeature_rate.mate2" DOUBLE, "seqdetective.sparsity.mate1" DOUBLE, "seqdetective.sparsity.mate2" DOUBLE, "seqdetective.pos_strand_rate.mate1" DOUBLE, "seqdetective.pos_strand_rate.mate2" DOUBLE, "seqdetective.readlen.mate1" BIGINT, "seqdetective.readlen.mate2" BIGINT, "seqdetective.judgement.mate1" VARCHAR, "seqdetective.judgement.mate2" VARCHAR, "seqdetective.judgement.reason" VARCHAR, platform_family VARCHAR, instrument_generation VARCHAR, read_bias VARCHAR, selection_class VARCHAR, prep_kit VARCHAR, sc_or_bulk VARCHAR, tech_class VARCHAR, technology VARCHAR, tech_variant VARCHAR, "submission.bioprojectsource.country" VARCHAR, earliest_date DATE, devstage_curation VARCHAR, devstage_curation_coarse VARCHAR, tissue_curation VARCHAR, tissue_curation_coarse VARCHAR);;
CREATE INDEX idx_run_bioproject ON run_metadata(bioproject);;
CREATE INDEX idx_run_run_accession ON run_metadata("run.accession");;