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run_metadata

115 rows where devstage_curation = "Undetermined" and tissue_curation = "Cell Line"

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experiment.library_selection 4

experiment.library_strategy 3

technology 2

experiment.library_layout 2

devstage_curation_coarse 2

tissue_curation_coarse 1

tissue_curation 1

  • Cell Line · 115 ✖

experiment.platform 1

experiment.library_source 1

devstage_curation 1

  • Undetermined · 115 ✖
Link rowid ▼ run.accession experiment.accession sample.accession study.accession bioproject study.title study.alias study.type study.abstract study.attributes study.PMIDs sample.description sample.title sample.alias sample.centername sample.attributes GEOsample.title GEOsample.dataprocessing GEOsample.source GEOsample.treatmentprotocol GEOsample.extractprotocol GEOsample.growthprotocol GEOsample.characteristics GEOsample.accession experiment.title experiment.alias experiment.library_name experiment.design_description experiment.library_construction_protocol experiment.attributes experiment.library_strategy experiment.library_source experiment.library_selection experiment.library_layout experiment.platform experiment.instrument_model experiment.spot_descriptor experiment.study_ref run.title run.attributes run.filename run.semantic_name run.total_bases run.total_spots run.alias run.read_lengths run.base_counts run.r1_length run.r2_length run.r3_length run.r4_length run.Acount run.Ccount run.Gcount run.Tcount run.Ncount run.experiment run.pool_member submission.accession submission.srasource submission.bioprojectsource seqdetective.n_mates seqdetective.mapping_rate.mate1 seqdetective.mapping_rate.mate2 seqdetective.nofeature_rate.mate1 seqdetective.nofeature_rate.mate2 seqdetective.sparsity.mate1 seqdetective.sparsity.mate2 seqdetective.pos_strand_rate.mate1 seqdetective.pos_strand_rate.mate2 seqdetective.readlen.mate1 seqdetective.readlen.mate2 seqdetective.judgement.mate1 seqdetective.judgement.mate2 seqdetective.judgement.reason platform_family instrument_generation read_bias selection_class prep_kit sc_or_bulk tech_class technology tech_variant submission.bioprojectsource.country earliest_date devstage_curation devstage_curation_coarse tissue_curation tissue_curation_coarse
28458 28458 SRR26265233 SRX21974767 SRS19050630 SRP464312 PRJNA1022576 Danio rerio Raw sequence reads PRJNA1022576 Whole Genome Sequencing In teleost type I IFNs are categorized into 2 subgroups containing one or two pairs of disulphide bond. However their functional differences have not been fully unveiled. It has been shown that IFN1 can be induced by viruses and trigger strong antiviral response in inducing hundreds of IFN stimulated genes conferring cell resistance to viruses. However the antiviral functions of IFN4 have been debated. To investigate the genes modulated by IFN1 and IFN4 ZF4 cells were stimulated with recombinant IFN1 and IFN4 and were performed transcriptome analysis.         IFN4 2   strain:not collected|age:not collected|collection date:2023 08 20|geo loc name:not collected|sex:not collected|tissue:not collected|cell line:ZF4|replicate:biological replicate 10|BioSampleModel:Model organism or animal                 RNAseq of zebrafish IFND2 IFND2 normal RNA seq of zebrafish     RNA-Seq TRANSCRIPTOMIC Oligo-dT PAIRED ILLUMINA Illumina NovaSeq 6000   SRP464312     IFN4-2.R2.fq.gz IFN4-2.R1.fq.gz fastq fastq 6934783900.0 23533465.0 IFN4 2.R1.fq.gz 0:147.35 1:147.33 A:1868083790;C:1590902416;G:1598661179;T:1876555558;N:580957 147 147     1868083790 1590902416 1598661179 1876555558 580957 SRX21974767 SRS19050630 SRA1725060 Shanghai Ocean University|College of Fisheries and Life Science Shanghai Ocean University 2 0.95226 0.95256 0.09237 0.09199 0.73403 0.73472 0.50112 0.4946 150 150 B B biological fallback assumption illumina novaseq_era unknown poly_a unknown bulk unknown unknown   China 2023-10-03 Undetermined Undetermined Cell Line Cell Line
28459 28459 SRR26265234 SRX21974766 SRS19050629 SRP464312 PRJNA1022576 Danio rerio Raw sequence reads PRJNA1022576 Whole Genome Sequencing In teleost type I IFNs are categorized into 2 subgroups containing one or two pairs of disulphide bond. However their functional differences have not been fully unveiled. It has been shown that IFN1 can be induced by viruses and trigger strong antiviral response in inducing hundreds of IFN stimulated genes conferring cell resistance to viruses. However the antiviral functions of IFN4 have been debated. To investigate the genes modulated by IFN1 and IFN4 ZF4 cells were stimulated with recombinant IFN1 and IFN4 and were performed transcriptome analysis.         IFN4 1   strain:not collected|age:not collected|collection date:2023 08 20|geo loc name:not collected|sex:not collected|tissue:not collected|cell line:ZF4|replicate:biological replicate 9|BioSampleModel:Model organism or animal                 RNAseq of zebrafish IFND1 IFND1 normal RNA seq of zebrafish     RNA-Seq TRANSCRIPTOMIC Oligo-dT PAIRED ILLUMINA Illumina NovaSeq 6000   SRP464312     IFN4-1.R2.fq.gz IFN4-1.R1.fq.gz fastq fastq 6955503146.0 23557276.0 IFN4 1.R1.fq.gz 0:147.65 1:147.61 A:1873984869;C:1595727817;G:1603714470;T:1881209728;N:866262 147 147     1873984869 1595727817 1603714470 1881209728 866262 SRX21974766 SRS19050629 SRA1725060 Shanghai Ocean University|College of Fisheries and Life Science Shanghai Ocean University 2 0.95329 0.95472 0.09371 0.09311 0.73535 0.7362 0.49542 0.49872 150 150 B B biological fallback assumption illumina novaseq_era unknown poly_a unknown bulk unknown unknown   China 2023-10-03 Undetermined Undetermined Cell Line Cell Line
28460 28460 SRR26265235 SRX21974765 SRS19050628 SRP464312 PRJNA1022576 Danio rerio Raw sequence reads PRJNA1022576 Whole Genome Sequencing In teleost type I IFNs are categorized into 2 subgroups containing one or two pairs of disulphide bond. However their functional differences have not been fully unveiled. It has been shown that IFN1 can be induced by viruses and trigger strong antiviral response in inducing hundreds of IFN stimulated genes conferring cell resistance to viruses. However the antiviral functions of IFN4 have been debated. To investigate the genes modulated by IFN1 and IFN4 ZF4 cells were stimulated with recombinant IFN1 and IFN4 and were performed transcriptome analysis.         IFN1 4   strain:not collected|age:not collected|collection date:2023 08 20|geo loc name:not collected|sex:not collected|tissue:not collected|cell line:ZF4|replicate:biological replicate 8|BioSampleModel:Model organism or animal                 RNAseq of zebrafish IFNA4 IFNA4 normal RNA seq of zebrafish     RNA-Seq TRANSCRIPTOMIC Oligo-dT PAIRED ILLUMINA Illumina NovaSeq 6000   SRP464312     IFN1-4.R1.fq.gz IFN1-4.R2.fq.gz fastq fastq 6497442039.0 22025966.0 IFN1 4.R1.fq.gz 0:147.50 1:147.49 A:1752434033;C:1488528327;G:1495225910;T:1760699235;N:554534 147 147     1752434033 1488528327 1495225910 1760699235 554534 SRX21974765 SRS19050628 SRA1725060 Shanghai Ocean University|College of Fisheries and Life Science Shanghai Ocean University 2 0.95168 0.95288 0.09073 0.09058 0.73691 0.73697 0.49659 0.49706 150 150 B B biological fallback assumption illumina novaseq_era unknown poly_a unknown bulk unknown unknown   China 2023-10-03 Undetermined Undetermined Cell Line Cell Line
28461 28461 SRR26265236 SRX21974764 SRS19050627 SRP464312 PRJNA1022576 Danio rerio Raw sequence reads PRJNA1022576 Whole Genome Sequencing In teleost type I IFNs are categorized into 2 subgroups containing one or two pairs of disulphide bond. However their functional differences have not been fully unveiled. It has been shown that IFN1 can be induced by viruses and trigger strong antiviral response in inducing hundreds of IFN stimulated genes conferring cell resistance to viruses. However the antiviral functions of IFN4 have been debated. To investigate the genes modulated by IFN1 and IFN4 ZF4 cells were stimulated with recombinant IFN1 and IFN4 and were performed transcriptome analysis.         IFN1 3   strain:not collected|age:not collected|collection date:2023 08 20|geo loc name:not collected|sex:not collected|tissue:not collected|cell line:ZF4|replicate:biological replicate 7|BioSampleModel:Model organism or animal                 RNAseq of zebrafish IFNA3 IFNA3 normal RNA seq of zebrafish     RNA-Seq TRANSCRIPTOMIC Oligo-dT PAIRED ILLUMINA Illumina NovaSeq 6000   SRP464312     IFN1-3.R1.fq.gz IFN1-3.R2.fq.gz fastq fastq 6258791362.0 21164255.0 IFN1 3.R1.fq.gz 0:147.88 1:147.85 A:1687323085;C:1434335716;G:1441365307;T:1695236268;N:530986 147 147     1687323085 1434335716 1441365307 1695236268 530986 SRX21974764 SRS19050627 SRA1725060 Shanghai Ocean University|College of Fisheries and Life Science Shanghai Ocean University 2 0.95206 0.95262 0.09255 0.09194 0.73549 0.73503 0.49685 0.50433 150 150 B B biological fallback assumption illumina novaseq_era unknown poly_a unknown bulk unknown unknown   China 2023-10-03 Undetermined Undetermined Cell Line Cell Line
28462 28462 SRR26265237 SRX21974763 SRS19050626 SRP464312 PRJNA1022576 Danio rerio Raw sequence reads PRJNA1022576 Whole Genome Sequencing In teleost type I IFNs are categorized into 2 subgroups containing one or two pairs of disulphide bond. However their functional differences have not been fully unveiled. It has been shown that IFN1 can be induced by viruses and trigger strong antiviral response in inducing hundreds of IFN stimulated genes conferring cell resistance to viruses. However the antiviral functions of IFN4 have been debated. To investigate the genes modulated by IFN1 and IFN4 ZF4 cells were stimulated with recombinant IFN1 and IFN4 and were performed transcriptome analysis.         IFN1 2   strain:not collected|age:not collected|collection date:2023 08 20|geo loc name:not collected|sex:not collected|tissue:not collected|cell line:ZF4|replicate:biological replicate 6|BioSampleModel:Model organism or animal                 RNAseq of zebrafish IFNA2 IFNA2 normal RNA seq of zebrafish     RNA-Seq TRANSCRIPTOMIC Oligo-dT PAIRED ILLUMINA Illumina NovaSeq 6000   SRP464312     IFN1-2.R1.fq.gz IFN1-2.R2.fq.gz fastq fastq 5566110483.0 18875664.0 IFN1 2.R1.fq.gz 0:147.46 1:147.43 A:1498457518;C:1278011392;G:1284254482;T:1504942516;N:444575 147 147     1498457518 1278011392 1284254482 1504942516 444575 SRX21974763 SRS19050626 SRA1725060 Shanghai Ocean University|College of Fisheries and Life Science Shanghai Ocean University 2 0.95197 0.9524 0.09035 0.08962 0.73606 0.73634 0.50146 0.49994 150 150 B B biological fallback assumption illumina novaseq_era unknown poly_a unknown bulk unknown unknown   China 2023-10-03 Undetermined Undetermined Cell Line Cell Line
28463 28463 SRR26265238 SRX21974762 SRS19050625 SRP464312 PRJNA1022576 Danio rerio Raw sequence reads PRJNA1022576 Whole Genome Sequencing In teleost type I IFNs are categorized into 2 subgroups containing one or two pairs of disulphide bond. However their functional differences have not been fully unveiled. It has been shown that IFN1 can be induced by viruses and trigger strong antiviral response in inducing hundreds of IFN stimulated genes conferring cell resistance to viruses. However the antiviral functions of IFN4 have been debated. To investigate the genes modulated by IFN1 and IFN4 ZF4 cells were stimulated with recombinant IFN1 and IFN4 and were performed transcriptome analysis.         IFN1 1   strain:not collected|age:not collected|collection date:2023 08 20|geo loc name:not collected|sex:not collected|tissue:not collected|cell line:ZF4|replicate:biological replicate 5|BioSampleModel:Model organism or animal                 RNAseq of zebrafish IFNA1 IFNA1 normal RNA seq of zebrafish     RNA-Seq TRANSCRIPTOMIC Oligo-dT PAIRED ILLUMINA Illumina NovaSeq 6000   SRP464312     IFN1-1.R1.fq.gz IFN1-1.R2.fq.gz fastq fastq 6919610192.0 23425305.0 IFN1 1.R1.fq.gz 0:147.71 1:147.68 A:1863998722;C:1587286271;G:1595557648;T:1872190552;N:576999 147 147     1863998722 1587286271 1595557648 1872190552 576999 SRX21974762 SRS19050625 SRA1725060 Shanghai Ocean University|College of Fisheries and Life Science Shanghai Ocean University 2 0.95297 0.95392 0.08926 0.0886 0.73744 0.73716 0.48737 0.49536 150 150 B B biological fallback assumption illumina novaseq_era unknown poly_a unknown bulk unknown unknown   China 2023-10-03 Undetermined Undetermined Cell Line Cell Line
28464 28464 SRR26265239 SRX21974761 SRS19050624 SRP464312 PRJNA1022576 Danio rerio Raw sequence reads PRJNA1022576 Whole Genome Sequencing In teleost type I IFNs are categorized into 2 subgroups containing one or two pairs of disulphide bond. However their functional differences have not been fully unveiled. It has been shown that IFN1 can be induced by viruses and trigger strong antiviral response in inducing hundreds of IFN stimulated genes conferring cell resistance to viruses. However the antiviral functions of IFN4 have been debated. To investigate the genes modulated by IFN1 and IFN4 ZF4 cells were stimulated with recombinant IFN1 and IFN4 and were performed transcriptome analysis.         Control 4   strain:not collected|age:not collected|collection date:2023 08 20|geo loc name:not collected|sex:not collected|tissue:not collected|cell line:ZF4|replicate:biological replicate 4|BioSampleModel:Model organism or animal                 RNAseq of zebrafish C4 C4 normal RNA seq of zebrafish     RNA-Seq TRANSCRIPTOMIC Oligo-dT PAIRED ILLUMINA Illumina NovaSeq 6000   SRP464312     Control-4.R1.fq.gz Control-4.R2.fq.gz fastq fastq 7048754711.0 23843338.0 Control 4.R1.fq.gz 0:147.83 1:147.80 A:1886695953;C:1629137936;G:1637672845;T:1894536437;N:711540 147 147     1886695953 1629137936 1637672845 1894536437 711540 SRX21974761 SRS19050624 SRA1725060 Shanghai Ocean University|College of Fisheries and Life Science Shanghai Ocean University 2 0.9556 0.95723 0.09014 0.08984 0.73533 0.73643 0.50146 0.50134 150 150 B B biological fallback assumption illumina novaseq_era unknown poly_a unknown bulk unknown unknown   China 2023-10-03 Undetermined Undetermined Cell Line Cell Line
28465 28465 SRR26265240 SRX21974760 SRS19050623 SRP464312 PRJNA1022576 Danio rerio Raw sequence reads PRJNA1022576 Whole Genome Sequencing In teleost type I IFNs are categorized into 2 subgroups containing one or two pairs of disulphide bond. However their functional differences have not been fully unveiled. It has been shown that IFN1 can be induced by viruses and trigger strong antiviral response in inducing hundreds of IFN stimulated genes conferring cell resistance to viruses. However the antiviral functions of IFN4 have been debated. To investigate the genes modulated by IFN1 and IFN4 ZF4 cells were stimulated with recombinant IFN1 and IFN4 and were performed transcriptome analysis.         Control 3   strain:not collected|age:not collected|collection date:2023 08 20|geo loc name:not collected|sex:not collected|tissue:not collected|cell line:ZF4|replicate:biological replicate 3|BioSampleModel:Model organism or animal                 RNAseq of zebrafish C3 C3 normal RNA seq of zebrafish     RNA-Seq TRANSCRIPTOMIC Oligo-dT PAIRED ILLUMINA Illumina NovaSeq 6000   SRP464312     Control-3.R1.fq.gz Control-3.R2.fq.gz fastq fastq 6936430397.0 23495179.0 Control 3.R1.fq.gz 0:147.63 1:147.60 A:1861679698;C:1598594022;G:1606747561;T:1868699697;N:709419 147 147     1861679698 1598594022 1606747561 1868699697 709419 SRX21974760 SRS19050623 SRA1725060 Shanghai Ocean University|College of Fisheries and Life Science Shanghai Ocean University 2 0.95496 0.95472 0.09092 0.08958 0.73505 0.73586 0.50541 0.50256 150 150 B B biological fallback assumption illumina novaseq_era unknown poly_a unknown bulk unknown unknown   China 2023-10-03 Undetermined Undetermined Cell Line Cell Line
28466 28466 SRR26265241 SRX21974759 SRS19050622 SRP464312 PRJNA1022576 Danio rerio Raw sequence reads PRJNA1022576 Whole Genome Sequencing In teleost type I IFNs are categorized into 2 subgroups containing one or two pairs of disulphide bond. However their functional differences have not been fully unveiled. It has been shown that IFN1 can be induced by viruses and trigger strong antiviral response in inducing hundreds of IFN stimulated genes conferring cell resistance to viruses. However the antiviral functions of IFN4 have been debated. To investigate the genes modulated by IFN1 and IFN4 ZF4 cells were stimulated with recombinant IFN1 and IFN4 and were performed transcriptome analysis.         IFN4 4   strain:not collected|age:not collected|collection date:2023 08 20|geo loc name:not collected|sex:not collected|tissue:not collected|cell line:ZF4|replicate:biological replicate 12|BioSampleModel:Model organism or animal                 RNAseq of zebrafish IFND4 IFND4 normal RNA seq of zebrafish     RNA-Seq TRANSCRIPTOMIC Oligo-dT PAIRED ILLUMINA Illumina NovaSeq 6000   SRP464312     IFN4-4.R1.fq.gz IFN4-4.R2.fq.gz fastq fastq 6904025687.0 23391394.0 IFN4 4.R1.fq.gz 0:147.59 1:147.56 A:1858866141;C:1585020535;G:1592940944;T:1866550686;N:647381 147 147     1858866141 1585020535 1592940944 1866550686 647381 SRX21974759 SRS19050622 SRA1725060 Shanghai Ocean University|College of Fisheries and Life Science Shanghai Ocean University 2 0.95345 0.95394 0.08901 0.08834 0.73537 0.73596 0.50012 0.49674 150 150 B B biological fallback assumption illumina novaseq_era unknown poly_a unknown bulk unknown unknown   China 2023-10-03 Undetermined Undetermined Cell Line Cell Line
28467 28467 SRR26265242 SRX21974758 SRS19050621 SRP464312 PRJNA1022576 Danio rerio Raw sequence reads PRJNA1022576 Whole Genome Sequencing In teleost type I IFNs are categorized into 2 subgroups containing one or two pairs of disulphide bond. However their functional differences have not been fully unveiled. It has been shown that IFN1 can be induced by viruses and trigger strong antiviral response in inducing hundreds of IFN stimulated genes conferring cell resistance to viruses. However the antiviral functions of IFN4 have been debated. To investigate the genes modulated by IFN1 and IFN4 ZF4 cells were stimulated with recombinant IFN1 and IFN4 and were performed transcriptome analysis.         IFN4 3   strain:not collected|age:not collected|collection date:2023 08 20|geo loc name:not collected|sex:not collected|tissue:not collected|cell line:ZF4|replicate:biological replicate 11|BioSampleModel:Model organism or animal                 RNAseq of zebrafish IFND3 IFND3 normal RNA seq of zebrafish     RNA-Seq TRANSCRIPTOMIC Oligo-dT PAIRED ILLUMINA Illumina NovaSeq 6000   SRP464312     IFN4-3.R1.fq.gz IFN4-3.R2.fq.gz fastq fastq 6983243474.0 23627912.0 IFN4 3.R1.fq.gz 0:147.80 1:147.75 A:1880838380;C:1603492441;G:1611871993;T:1885974103;N:1066557 147 147     1880838380 1603492441 1611871993 1885974103 1066557 SRX21974758 SRS19050621 SRA1725060 Shanghai Ocean University|College of Fisheries and Life Science Shanghai Ocean University 2 0.95262 0.95399 0.09442 0.09269 0.73586 0.73519 0.50113 0.49912 150 150 B B biological fallback assumption illumina novaseq_era unknown poly_a unknown bulk unknown unknown   China 2023-10-03 Undetermined Undetermined Cell Line Cell Line
28468 28468 SRR26265243 SRX21974757 SRS19050620 SRP464312 PRJNA1022576 Danio rerio Raw sequence reads PRJNA1022576 Whole Genome Sequencing In teleost type I IFNs are categorized into 2 subgroups containing one or two pairs of disulphide bond. However their functional differences have not been fully unveiled. It has been shown that IFN1 can be induced by viruses and trigger strong antiviral response in inducing hundreds of IFN stimulated genes conferring cell resistance to viruses. However the antiviral functions of IFN4 have been debated. To investigate the genes modulated by IFN1 and IFN4 ZF4 cells were stimulated with recombinant IFN1 and IFN4 and were performed transcriptome analysis.         Control 2   strain:not collected|age:not collected|collection date:2023 08 20|geo loc name:not collected|sex:not collected|tissue:not collected|cell line:ZF4|replicate:biological replicate 2|BioSampleModel:Model organism or animal                 RNAseq of zebrafish C2 C2 normal RNA seq of zebrafish     RNA-Seq TRANSCRIPTOMIC Oligo-dT PAIRED ILLUMINA Illumina NovaSeq 6000   SRP464312     Control-2.R1.fq.gz Control-2.R2.fq.gz fastq fastq 7064416471.0 23901145.0 Control 2.R1.fq.gz 0:147.80 1:147.77 A:1896430007;C:1626943513;G:1635235356;T:1905174563;N:633032 147 147     1896430007 1626943513 1635235356 1905174563 633032 SRX21974757 SRS19050620 SRA1725060 Shanghai Ocean University|College of Fisheries and Life Science Shanghai Ocean University 2 0.95546 0.95678 0.08984 0.08994 0.73535 0.73565 0.49891 0.49705 150 150 B B biological fallback assumption illumina novaseq_era unknown poly_a unknown bulk unknown unknown   China 2023-10-03 Undetermined Undetermined Cell Line Cell Line
28469 28469 SRR26265244 SRX21974756 SRS19050619 SRP464312 PRJNA1022576 Danio rerio Raw sequence reads PRJNA1022576 Whole Genome Sequencing In teleost type I IFNs are categorized into 2 subgroups containing one or two pairs of disulphide bond. However their functional differences have not been fully unveiled. It has been shown that IFN1 can be induced by viruses and trigger strong antiviral response in inducing hundreds of IFN stimulated genes conferring cell resistance to viruses. However the antiviral functions of IFN4 have been debated. To investigate the genes modulated by IFN1 and IFN4 ZF4 cells were stimulated with recombinant IFN1 and IFN4 and were performed transcriptome analysis.         Control 1   strain:not collected|age:not collected|collection date:2023 08 20|geo loc name:not collected|sex:not collected|tissue:not collected|cell line:ZF4|replicate:biological replicate 1|BioSampleModel:Model organism or animal                 RNAseq of zebrafish C1 C1 normal RNA seq of zebrafish     RNA-Seq TRANSCRIPTOMIC Oligo-dT PAIRED ILLUMINA Illumina NovaSeq 6000   SRP464312     Control-1.R1.fq.gz Control-1.R2.fq.gz fastq fastq 7005585889.0 23733330.0 Control 1.R1.fq.gz 0:147.61 1:147.57 A:1874251473;C:1620035540;G:1628125464;T:1882607735;N:565677 147 147     1874251473 1620035540 1628125464 1882607735 565677 SRX21974756 SRS19050619 SRA1725060 Shanghai Ocean University|College of Fisheries and Life Science Shanghai Ocean University 2 0.95539 0.95612 0.08699 0.08663 0.73777 0.73833 0.49693 0.49816 150 150 B B biological fallback assumption illumina novaseq_era unknown poly_a unknown bulk unknown unknown   China 2023-10-03 Undetermined Undetermined Cell Line Cell Line
28470 28470 SRR26253203 SRX21963295 SRS19039869 SRP464138 PRJNA1022587 Danio rerio Raw sequence reads PRJNA1022587 Whole Genome Sequencing In teleost type I IFNs are categorized into 2 subgroups containing one or two pairs of disulphide bond. However their functional differences have not been fully unveiled. It has been shown that IFN1 can be induced by viruses and trigger strong antiviral response in inducing hundreds of IFN stimulated genes conferring cell resistance to viruses. However the antiviral functions of IFN4 have been debated. To investigate the genes modulated by IFN1 and IFN4 ZF4 cells were stimulated with recombinant IFN1 and IFN4 and were performed transcriptome analysis of the small RNA.         Small RNA IFN4 2   strain:not collected|age:not collected|collection date:2023 08 20|geo loc name:not collected|sex:not collected|tissue:not collected|cell line:ZF4|replicate:biological replicate 22|BioSampleModel:Model organism or animal                 Small RNAseq of zebrafish Small RNA IFND2 Small RNA IFND2 small RNA seq of zebrafish     miRNA-Seq TRANSCRIPTOMIC PCR SINGLE ILLUMINA Illumina NovaSeq 6000   SRP464138     IFN4-2.deadaptor.fq.gz fastq 278362309.0 11056139.0 IFN4 2.deadaptor.fq.gz 0:25.18 A:71944968;C:54562141;G:73333701;T:78511075;N:10424 25       71944968 54562141 73333701 78511075 10424 SRX21963295 SRS19039869 SRA1724093 Shanghai Ocean University|College of Fisheries and Life Science Shanghai Ocean University 1 0.93133   0.07401   0.96181   0.74558   31   B   usable mapping rate illumina novaseq_era unknown random_priming unknown bulk unknown unknown   China 2023-10-02 Undetermined Undetermined Cell Line Cell Line
28471 28471 SRR26253204 SRX21963294 SRS19039868 SRP464138 PRJNA1022587 Danio rerio Raw sequence reads PRJNA1022587 Whole Genome Sequencing In teleost type I IFNs are categorized into 2 subgroups containing one or two pairs of disulphide bond. However their functional differences have not been fully unveiled. It has been shown that IFN1 can be induced by viruses and trigger strong antiviral response in inducing hundreds of IFN stimulated genes conferring cell resistance to viruses. However the antiviral functions of IFN4 have been debated. To investigate the genes modulated by IFN1 and IFN4 ZF4 cells were stimulated with recombinant IFN1 and IFN4 and were performed transcriptome analysis of the small RNA.         Small RNA IFN4 1   strain:not collected|age:not collected|collection date:2023 08 20|geo loc name:not collected|sex:not collected|tissue:not collected|cell line:ZF4|replicate:biological replicate 21|BioSampleModel:Model organism or animal                 Small RNAseq of zebrafish Small RNA IFND1 Small RNA IFND1 small RNA seq of zebrafish     miRNA-Seq TRANSCRIPTOMIC PCR SINGLE ILLUMINA Illumina NovaSeq 6000   SRP464138     IFN4-1.deadaptor.fq.gz fastq 289362334.0 11048590.0 IFN4 1.deadaptor.fq.gz 0:26.19 A:73933649;C:59386039;G:76847534;T:79184583;N:10529 26       73933649 59386039 76847534 79184583 10529 SRX21963294 SRS19039868 SRA1724093 Shanghai Ocean University|College of Fisheries and Life Science Shanghai Ocean University 1 0.91683   0.07099   0.96132   0.64882   25   B   usable mapping rate illumina novaseq_era unknown random_priming unknown bulk unknown unknown   China 2023-10-02 Undetermined Undetermined Cell Line Cell Line
28472 28472 SRR26253205 SRX21963293 SRS19039867 SRP464138 PRJNA1022587 Danio rerio Raw sequence reads PRJNA1022587 Whole Genome Sequencing In teleost type I IFNs are categorized into 2 subgroups containing one or two pairs of disulphide bond. However their functional differences have not been fully unveiled. It has been shown that IFN1 can be induced by viruses and trigger strong antiviral response in inducing hundreds of IFN stimulated genes conferring cell resistance to viruses. However the antiviral functions of IFN4 have been debated. To investigate the genes modulated by IFN1 and IFN4 ZF4 cells were stimulated with recombinant IFN1 and IFN4 and were performed transcriptome analysis of the small RNA.         Small RNA IFN1 4   strain:not collected|age:not collected|collection date:2023 08 20|geo loc name:not collected|sex:not collected|tissue:not collected|cell line:ZF4|replicate:biological replicate 20|BioSampleModel:Model organism or animal                 Small RNAseq of zebrafish Small RNA IFNA4 Small RNA IFNA4 small RNA seq of zebrafish     miRNA-Seq TRANSCRIPTOMIC PCR SINGLE ILLUMINA Illumina NovaSeq 6000   SRP464138     IFN1-4.deadaptor.fq.gz fastq 270529395.0 11070360.0 IFN1 4.deadaptor.fq.gz 0:24.44 A:69759888;C:54924667;G:71358091;T:74470720;N:16029 24       69759888 54924667 71358091 74470720 16029 SRX21963293 SRS19039867 SRA1724093 Shanghai Ocean University|College of Fisheries and Life Science Shanghai Ocean University 1 0.88469   0.06696   0.96664   0.74853   22   B   usable mapping rate illumina novaseq_era unknown random_priming unknown bulk unknown unknown   China 2023-10-02 Undetermined Undetermined Cell Line Cell Line
28473 28473 SRR26253206 SRX21963292 SRS19039866 SRP464138 PRJNA1022587 Danio rerio Raw sequence reads PRJNA1022587 Whole Genome Sequencing In teleost type I IFNs are categorized into 2 subgroups containing one or two pairs of disulphide bond. However their functional differences have not been fully unveiled. It has been shown that IFN1 can be induced by viruses and trigger strong antiviral response in inducing hundreds of IFN stimulated genes conferring cell resistance to viruses. However the antiviral functions of IFN4 have been debated. To investigate the genes modulated by IFN1 and IFN4 ZF4 cells were stimulated with recombinant IFN1 and IFN4 and were performed transcriptome analysis of the small RNA.         Small RNA IFN1 3   strain:not collected|age:not collected|collection date:2023 08 20|geo loc name:not collected|sex:not collected|tissue:not collected|cell line:ZF4|replicate:biological replicate 19|BioSampleModel:Model organism or animal                 Small RNAseq of zebrafish Small RNA IFNA3 Small RNA IFNA3 small RNA seq of zebrafish     miRNA-Seq TRANSCRIPTOMIC PCR SINGLE ILLUMINA Illumina NovaSeq 6000   SRP464138     IFN1-3.deadaptor.fq.gz fastq 353291957.0 11740560.0 IFN1 3.deadaptor.fq.gz 0:30.09 A:89026549;C:76490337;G:94719420;T:93036955;N:18696 30       89026549 76490337 94719420 93036955 18696 SRX21963292 SRS19039866 SRA1724093 Shanghai Ocean University|College of Fisheries and Life Science Shanghai Ocean University 1 0.83151   0.07199   0.96471   0.71641   22   B   usable mapping rate illumina novaseq_era unknown random_priming unknown bulk unknown unknown   China 2023-10-02 Undetermined Undetermined Cell Line Cell Line
28474 28474 SRR26253207 SRX21963291 SRS19039865 SRP464138 PRJNA1022587 Danio rerio Raw sequence reads PRJNA1022587 Whole Genome Sequencing In teleost type I IFNs are categorized into 2 subgroups containing one or two pairs of disulphide bond. However their functional differences have not been fully unveiled. It has been shown that IFN1 can be induced by viruses and trigger strong antiviral response in inducing hundreds of IFN stimulated genes conferring cell resistance to viruses. However the antiviral functions of IFN4 have been debated. To investigate the genes modulated by IFN1 and IFN4 ZF4 cells were stimulated with recombinant IFN1 and IFN4 and were performed transcriptome analysis of the small RNA.         Small RNA IFN1 2   strain:not collected|age:not collected|collection date:2023 08 20|geo loc name:not collected|sex:not collected|tissue:not collected|cell line:ZF4|replicate:biological replicate 18|BioSampleModel:Model organism or animal                 Small RNAseq of zebrafish Small RNA IFNA2 Small RNA IFNA2 small RNA seq of zebrafish     miRNA-Seq TRANSCRIPTOMIC PCR SINGLE ILLUMINA Illumina NovaSeq 6000   SRP464138     IFN1-2.deadaptor.fq.gz fastq 257949577.0 11213774.0 IFN1 2.deadaptor.fq.gz 0:23.00 A:67371681;C:51128621;G:67605590;T:71826657;N:17028 23       67371681 51128621 67605590 71826657 17028 SRX21963291 SRS19039865 SRA1724093 Shanghai Ocean University|College of Fisheries and Life Science Shanghai Ocean University 1 0.91396   0.06536   0.97197   0.76269   23   B   usable mapping rate illumina novaseq_era unknown random_priming unknown bulk unknown unknown   China 2023-10-02 Undetermined Undetermined Cell Line Cell Line
28475 28475 SRR26253208 SRX21963290 SRS19039864 SRP464138 PRJNA1022587 Danio rerio Raw sequence reads PRJNA1022587 Whole Genome Sequencing In teleost type I IFNs are categorized into 2 subgroups containing one or two pairs of disulphide bond. However their functional differences have not been fully unveiled. It has been shown that IFN1 can be induced by viruses and trigger strong antiviral response in inducing hundreds of IFN stimulated genes conferring cell resistance to viruses. However the antiviral functions of IFN4 have been debated. To investigate the genes modulated by IFN1 and IFN4 ZF4 cells were stimulated with recombinant IFN1 and IFN4 and were performed transcriptome analysis of the small RNA.         Small RNA IFN1 1   strain:not collected|age:not collected|collection date:2023 08 20|geo loc name:not collected|sex:not collected|tissue:not collected|cell line:ZF4|replicate:biological replicate 17|BioSampleModel:Model organism or animal                 Small RNAseq of zebrafish Small RNA IFNA1 Small RNA IFNA1 small RNA seq of zebrafish     miRNA-Seq TRANSCRIPTOMIC PCR SINGLE ILLUMINA Illumina NovaSeq 6000   SRP464138     IFN1-1.deadaptor.fq.gz fastq 329358383.0 11799176.0 IFN1 1.deadaptor.fq.gz 0:27.91 A:84093681;C:68961271;G:86502739;T:89779605;N:21087 27       84093681 68961271 86502739 89779605 21087 SRX21963290 SRS19039864 SRA1724093 Shanghai Ocean University|College of Fisheries and Life Science Shanghai Ocean University 1 0.92116   0.07483   0.96796   0.75179   22   B   usable mapping rate illumina novaseq_era unknown random_priming unknown bulk unknown unknown   China 2023-10-02 Undetermined Undetermined Cell Line Cell Line
28476 28476 SRR26253209 SRX21963289 SRS19039863 SRP464138 PRJNA1022587 Danio rerio Raw sequence reads PRJNA1022587 Whole Genome Sequencing In teleost type I IFNs are categorized into 2 subgroups containing one or two pairs of disulphide bond. However their functional differences have not been fully unveiled. It has been shown that IFN1 can be induced by viruses and trigger strong antiviral response in inducing hundreds of IFN stimulated genes conferring cell resistance to viruses. However the antiviral functions of IFN4 have been debated. To investigate the genes modulated by IFN1 and IFN4 ZF4 cells were stimulated with recombinant IFN1 and IFN4 and were performed transcriptome analysis of the small RNA.         Small RNA Control 4   strain:not collected|age:not collected|collection date:2023 08 20|geo loc name:not collected|sex:not collected|tissue:not collected|cell line:ZF4|replicate:biological replicate 16|BioSampleModel:Model organism or animal                 Small RNAseq of zebrafish Small RNA C4 Small RNA C4 small RNA seq of zebrafish     miRNA-Seq TRANSCRIPTOMIC PCR SINGLE ILLUMINA Illumina NovaSeq 6000   SRP464138     Control-4.deadaptor.fq.gz fastq 251577736.0 10951659.0 Control 4.deadaptor.fq.gz 0:22.97 A:65382449;C:50041232;G:66038625;T:70106464;N:8966 22       65382449 50041232 66038625 70106464 8966 SRX21963289 SRS19039863 SRA1724093 Shanghai Ocean University|College of Fisheries and Life Science Shanghai Ocean University 1 0.89744   0.06821   0.96607   0.63829   22   B   usable mapping rate illumina novaseq_era unknown random_priming unknown bulk unknown unknown   China 2023-10-02 Undetermined Undetermined Cell Line Cell Line
28477 28477 SRR26253210 SRX21963288 SRS19039862 SRP464138 PRJNA1022587 Danio rerio Raw sequence reads PRJNA1022587 Whole Genome Sequencing In teleost type I IFNs are categorized into 2 subgroups containing one or two pairs of disulphide bond. However their functional differences have not been fully unveiled. It has been shown that IFN1 can be induced by viruses and trigger strong antiviral response in inducing hundreds of IFN stimulated genes conferring cell resistance to viruses. However the antiviral functions of IFN4 have been debated. To investigate the genes modulated by IFN1 and IFN4 ZF4 cells were stimulated with recombinant IFN1 and IFN4 and were performed transcriptome analysis of the small RNA.         Small RNA Control 3   strain:not collected|age:not collected|collection date:2023 08 20|geo loc name:not collected|sex:not collected|tissue:not collected|cell line:ZF4|replicate:biological replicate 15|BioSampleModel:Model organism or animal                 Small RNAseq of zebrafish Small RNA C3 Small RNA C3 small RNA seq of zebrafish     miRNA-Seq TRANSCRIPTOMIC PCR SINGLE ILLUMINA Illumina NovaSeq 6000   SRP464138     Control-3.deadaptor.fq.gz fastq 252164979.0 11004167.0 Control 3.deadaptor.fq.gz 0:22.92 A:65850899;C:48466665;G:66708038;T:71130786;N:8591 22       65850899 48466665 66708038 71130786 8591 SRX21963288 SRS19039862 SRA1724093 Shanghai Ocean University|College of Fisheries and Life Science Shanghai Ocean University 1 0.91567   0.06804   0.96278   0.75579   22   B   usable mapping rate illumina novaseq_era unknown random_priming unknown bulk unknown unknown   China 2023-10-02 Undetermined Undetermined Cell Line Cell Line
28478 28478 SRR26253211 SRX21963287 SRS19039861 SRP464138 PRJNA1022587 Danio rerio Raw sequence reads PRJNA1022587 Whole Genome Sequencing In teleost type I IFNs are categorized into 2 subgroups containing one or two pairs of disulphide bond. However their functional differences have not been fully unveiled. It has been shown that IFN1 can be induced by viruses and trigger strong antiviral response in inducing hundreds of IFN stimulated genes conferring cell resistance to viruses. However the antiviral functions of IFN4 have been debated. To investigate the genes modulated by IFN1 and IFN4 ZF4 cells were stimulated with recombinant IFN1 and IFN4 and were performed transcriptome analysis of the small RNA.         Small RNA IFN4 4   strain:not collected|age:not collected|collection date:2023 08 20|geo loc name:not collected|sex:not collected|tissue:not collected|cell line:ZF4|replicate:biological replicate 24|BioSampleModel:Model organism or animal                 Small RNAseq of zebrafish Small RNA IFND4 Small RNA IFND4 small RNA seq of zebrafish     miRNA-Seq TRANSCRIPTOMIC PCR SINGLE ILLUMINA Illumina NovaSeq 6000   SRP464138     IFN4-4.deadaptor.fq.gz fastq 291708053.0 11503883.0 IFN4 4.deadaptor.fq.gz 0:25.36 A:74079231;C:61368459;G:76898572;T:79351225;N:10566 25       74079231 61368459 76898572 79351225 10566 SRX21963287 SRS19039861 SRA1724093 Shanghai Ocean University|College of Fisheries and Life Science Shanghai Ocean University 1 0.88888   0.0633   0.97305   0.74862   23   B   usable mapping rate illumina novaseq_era unknown random_priming unknown bulk unknown unknown   China 2023-10-02 Undetermined Undetermined Cell Line Cell Line
28479 28479 SRR26253212 SRX21963286 SRS19039860 SRP464138 PRJNA1022587 Danio rerio Raw sequence reads PRJNA1022587 Whole Genome Sequencing In teleost type I IFNs are categorized into 2 subgroups containing one or two pairs of disulphide bond. However their functional differences have not been fully unveiled. It has been shown that IFN1 can be induced by viruses and trigger strong antiviral response in inducing hundreds of IFN stimulated genes conferring cell resistance to viruses. However the antiviral functions of IFN4 have been debated. To investigate the genes modulated by IFN1 and IFN4 ZF4 cells were stimulated with recombinant IFN1 and IFN4 and were performed transcriptome analysis of the small RNA.         Small RNA IFN4 3   strain:not collected|age:not collected|collection date:2023 08 20|geo loc name:not collected|sex:not collected|tissue:not collected|cell line:ZF4|replicate:biological replicate 23|BioSampleModel:Model organism or animal                 Small RNAseq of zebrafish Small RNA IFND3 Small RNA IFND3 small RNA seq of zebrafish     miRNA-Seq TRANSCRIPTOMIC PCR SINGLE ILLUMINA Illumina NovaSeq 6000   SRP464138     IFN4-3.deadaptor.fq.gz fastq 349995495.0 11885662.0 IFN4 3.deadaptor.fq.gz 0:29.45 A:87443709;C:76373108;G:92741296;T:93425866;N:11516 29       87443709 76373108 92741296 93425866 11516 SRX21963286 SRS19039860 SRA1724093 Shanghai Ocean University|College of Fisheries and Life Science Shanghai Ocean University 1 0.92619   0.06843   0.9709   0.74977   73   B   usable mapping rate illumina novaseq_era unknown random_priming unknown bulk unknown unknown   China 2023-10-02 Undetermined Undetermined Cell Line Cell Line
28480 28480 SRR26253213 SRX21963285 SRS19039859 SRP464138 PRJNA1022587 Danio rerio Raw sequence reads PRJNA1022587 Whole Genome Sequencing In teleost type I IFNs are categorized into 2 subgroups containing one or two pairs of disulphide bond. However their functional differences have not been fully unveiled. It has been shown that IFN1 can be induced by viruses and trigger strong antiviral response in inducing hundreds of IFN stimulated genes conferring cell resistance to viruses. However the antiviral functions of IFN4 have been debated. To investigate the genes modulated by IFN1 and IFN4 ZF4 cells were stimulated with recombinant IFN1 and IFN4 and were performed transcriptome analysis of the small RNA.         Small RNA Control 2   strain:not collected|age:not collected|collection date:2023 08 20|geo loc name:not collected|sex:not collected|tissue:not collected|cell line:ZF4|replicate:biological replicate 14|BioSampleModel:Model organism or animal                 Small RNAseq of zebrafish Small RNA C2 Small RNA C2 small RNA seq of zebrafish     miRNA-Seq TRANSCRIPTOMIC PCR SINGLE ILLUMINA Illumina NovaSeq 6000   SRP464138     Control-2.deadaptor.fq.gz fastq 254163676.0 10744164.0 Control 2.deadaptor.fq.gz 0:23.66 A:66003417;C:49309846;G:67463940;T:71377644;N:8829 23       66003417 49309846 67463940 71377644 8829 SRX21963285 SRS19039859 SRA1724093 Shanghai Ocean University|College of Fisheries and Life Science Shanghai Ocean University 1 0.93068   0.06626   0.96441   0.7492   22   B   usable mapping rate illumina novaseq_era unknown random_priming unknown bulk unknown unknown   China 2023-10-02 Undetermined Undetermined Cell Line Cell Line
28481 28481 SRR26253214 SRX21963284 SRS19039858 SRP464138 PRJNA1022587 Danio rerio Raw sequence reads PRJNA1022587 Whole Genome Sequencing In teleost type I IFNs are categorized into 2 subgroups containing one or two pairs of disulphide bond. However their functional differences have not been fully unveiled. It has been shown that IFN1 can be induced by viruses and trigger strong antiviral response in inducing hundreds of IFN stimulated genes conferring cell resistance to viruses. However the antiviral functions of IFN4 have been debated. To investigate the genes modulated by IFN1 and IFN4 ZF4 cells were stimulated with recombinant IFN1 and IFN4 and were performed transcriptome analysis of the small RNA.         Small RNA Control 1   strain:not collected|age:not collected|collection date:2023 08 20|geo loc name:not collected|sex:not collected|tissue:not collected|cell line:ZF4|replicate:biological replicate 13|BioSampleModel:Model organism or animal                 Small RNAseq of zebrafish Small RNA C1 Small RNA C1 small RNA seq of zebrafish     miRNA-Seq TRANSCRIPTOMIC PCR SINGLE ILLUMINA Illumina NovaSeq 6000   SRP464138     Control-1.deadaptor.fq.gz fastq 267090429.0 11027241.0 Control 1.deadaptor.fq.gz 0:24.22 A:70162511;C:50705437;G:69872659;T:76339682;N:10140 24       70162511 50705437 69872659 76339682 10140 SRX21963284 SRS19039858 SRA1724093 Shanghai Ocean University|College of Fisheries and Life Science Shanghai Ocean University 1 0.93267   0.07189   0.96735   0.75447   21   B   usable mapping rate illumina novaseq_era unknown random_priming unknown bulk unknown unknown   China 2023-10-02 Undetermined Undetermined Cell Line Cell Line
28985 28985 SRR26936267 SRX22630108 SRS19628590 SRP473892 PRJNA1044439 Social stress in fathers affects sperm small RNA and offspring transcriptome profiles GSE248535 Transcriptome Analysis Environmental changes may affect paternal condition and following generations but the underlying mechanisms are poorly understood. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and development in their offspring. Here we investigated the role of sperm mediated small RNAs in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes and transposable elements TEs at this key developmental stage. We sequenced micro mi and Piwi interacting piRNAs in sperm and the full transcriptome in the embryos and ran differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from high to low competition environments. We identified 612 differentially expressed genes in the embryos. These results confirm that the social environment does not only affect males but also the molecular ecology of their sperm and the gene expression in their offspring suggesting a putative role of sRNAs. Overall design: This data deposit includes the small RNA seq data for this research. Male zebrafish were subject to high and low social stress environments with sperm samples collected RNA extracted and sent for small RNA sequencing.   pubmed:40121340   La 6 GSM7916507   tissue:sperm|cell line:sperm|genotype:WT|treatment:Low2|geo loc name:missing|collection date:missing La 6 Reads were trimmed aligned with PatMan and then count matrices assembled before DESeq2 analyses Assembly: GRCz10 Supplementary files format and content: piRNA raw counts.csv raw counts of a subset of 24 out of the 40 samples that were analysed. These 24 were chosen due to noise observed in PCA clustering. Supplementary files format and content: mirna raw counts.csv raw counts for 39/40 samples as one sample failed to progress through our pipeline to the same high quality as the other 39 so this sample was omitted from analyses. sperm   Total RNA extraction New England BioLabs kit NEBNext®Multiplex Small RNA Library Prep Set for Illumina® Set 1 and 2 NEB #E7300 and NEB #E7580 small RNA library prep for Illumina   cell line:sperm|genotype:WT|treatment:Low2 GSM7916507 GSM7916507: La 6; Danio rerio; ncRNA Seq GSM7916507 r1 GSM7916507 1 Total RNA extraction New England BioLabs kit NEBNext®Multiplex Small RNA Library Prep Set for Illumina® Set 1 and 2 NEB #E7300 and NEB #E7580 small RNA library prep for Illumina   ncRNA-Seq TRANSCRIPTOMIC size fractionation SINGLE ILLUMINA Illumina HiSeq 2500   SRP473892     12235X32_160303_D00294_0224_AC95NJANXX_7.cutadapt.se.19_100.fastq.gz fastq 810705967.0 27544418.0 GSM7916507 r1 0:29.43 A:141437993;C:173890211;G:258408531;T:236922660;N:46572 29       141437993 173890211 258408531 236922660 46572 SRX22630108 SRS19628590 SRA1756943 University of East Anglia University of East Anglia 1 0.86832   0.1884   0.84735   0.64657   21   B   usable mapping rate illumina hiseq_era unknown size_fractionation nebnext bulk unknown unknown   United Kingdom 2023-11-23 Undetermined Embryo Cell Line Cell Line
28986 28986 SRR26936231 SRX22630107 SRS19628589 SRP473892 PRJNA1044439 Social stress in fathers affects sperm small RNA and offspring transcriptome profiles GSE248535 Transcriptome Analysis Environmental changes may affect paternal condition and following generations but the underlying mechanisms are poorly understood. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and development in their offspring. Here we investigated the role of sperm mediated small RNAs in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes and transposable elements TEs at this key developmental stage. We sequenced micro mi and Piwi interacting piRNAs in sperm and the full transcriptome in the embryos and ran differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from high to low competition environments. We identified 612 differentially expressed genes in the embryos. These results confirm that the social environment does not only affect males but also the molecular ecology of their sperm and the gene expression in their offspring suggesting a putative role of sRNAs. Overall design: This data deposit includes the small RNA seq data for this research. Male zebrafish were subject to high and low social stress environments with sperm samples collected RNA extracted and sent for small RNA sequencing.   pubmed:40121340   La 5 GSM7916506   tissue:sperm|cell line:sperm|genotype:WT|treatment:Low1|geo loc name:missing|collection date:missing La 5 Reads were trimmed aligned with PatMan and then count matrices assembled before DESeq2 analyses Assembly: GRCz10 Supplementary files format and content: piRNA raw counts.csv raw counts of a subset of 24 out of the 40 samples that were analysed. These 24 were chosen due to noise observed in PCA clustering. Supplementary files format and content: mirna raw counts.csv raw counts for 39/40 samples as one sample failed to progress through our pipeline to the same high quality as the other 39 so this sample was omitted from analyses. sperm   Total RNA extraction New England BioLabs kit NEBNext®Multiplex Small RNA Library Prep Set for Illumina® Set 1 and 2 NEB #E7300 and NEB #E7580 small RNA library prep for Illumina   cell line:sperm|genotype:WT|treatment:Low1 GSM7916506 GSM7916506: La 5; Danio rerio; ncRNA Seq GSM7916506 r1 GSM7916506 1 Total RNA extraction New England BioLabs kit NEBNext®Multiplex Small RNA Library Prep Set for Illumina® Set 1 and 2 NEB #E7300 and NEB #E7580 small RNA library prep for Illumina   ncRNA-Seq TRANSCRIPTOMIC size fractionation SINGLE ILLUMINA Illumina HiSeq 2500   SRP473892     12235X31_160303_D00294_0224_AC95NJANXX_7.cutadapt.se.19_100.fastq.gz fastq 1237906972.0 45149037.0 GSM7916506 r1 0:27.42 A:273044213;C:252818121;G:368519115;T:343444331;N:81192 27       273044213 252818121 368519115 343444331 81192 SRX22630107 SRS19628589 SRA1756943 University of East Anglia University of East Anglia 1 0.86015   0.25463   0.83798   0.67166   25   B   usable mapping rate illumina hiseq_era unknown size_fractionation nebnext bulk unknown unknown   United Kingdom 2023-11-23 Undetermined Embryo Cell Line Cell Line
28987 28987 SRR26936232 SRX22630106 SRS19628588 SRP473892 PRJNA1044439 Social stress in fathers affects sperm small RNA and offspring transcriptome profiles GSE248535 Transcriptome Analysis Environmental changes may affect paternal condition and following generations but the underlying mechanisms are poorly understood. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and development in their offspring. Here we investigated the role of sperm mediated small RNAs in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes and transposable elements TEs at this key developmental stage. We sequenced micro mi and Piwi interacting piRNAs in sperm and the full transcriptome in the embryos and ran differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from high to low competition environments. We identified 612 differentially expressed genes in the embryos. These results confirm that the social environment does not only affect males but also the molecular ecology of their sperm and the gene expression in their offspring suggesting a putative role of sRNAs. Overall design: This data deposit includes the small RNA seq data for this research. Male zebrafish were subject to high and low social stress environments with sperm samples collected RNA extracted and sent for small RNA sequencing.   pubmed:40121340   Hb 2 GSM7916505   tissue:sperm|cell line:sperm|genotype:WT|treatment:High2|geo loc name:missing|collection date:missing Hb 2 Reads were trimmed aligned with PatMan and then count matrices assembled before DESeq2 analyses Assembly: GRCz10 Supplementary files format and content: piRNA raw counts.csv raw counts of a subset of 24 out of the 40 samples that were analysed. These 24 were chosen due to noise observed in PCA clustering. Supplementary files format and content: mirna raw counts.csv raw counts for 39/40 samples as one sample failed to progress through our pipeline to the same high quality as the other 39 so this sample was omitted from analyses. sperm   Total RNA extraction New England BioLabs kit NEBNext®Multiplex Small RNA Library Prep Set for Illumina® Set 1 and 2 NEB #E7300 and NEB #E7580 small RNA library prep for Illumina   cell line:sperm|genotype:WT|treatment:High2 GSM7916505 GSM7916505: Hb 2; Danio rerio; ncRNA Seq GSM7916505 r1 GSM7916505 1 Total RNA extraction New England BioLabs kit NEBNext®Multiplex Small RNA Library Prep Set for Illumina® Set 1 and 2 NEB #E7300 and NEB #E7580 small RNA library prep for Illumina   ncRNA-Seq TRANSCRIPTOMIC size fractionation SINGLE ILLUMINA Illumina HiSeq 2500   SRP473892     12235X30_160303_D00294_0224_AC95NJANXX_7.cutadapt.se.19_100.fastq.gz fastq 214730387.0 7217086.0 GSM7916505 r1 0:29.75 A:42819722;C:48080032;G:67677978;T:56140049;N:12606 29       42819722 48080032 67677978 56140049 12606 SRX22630106 SRS19628588 SRA1756943 University of East Anglia University of East Anglia 1 0.85212   0.1617   0.86856   0.69163   50   B   usable mapping rate illumina hiseq_era unknown size_fractionation nebnext bulk unknown unknown   United Kingdom 2023-11-23 Undetermined Embryo Cell Line Cell Line
28988 28988 SRR26936233 SRX22630105 SRS19628587 SRP473892 PRJNA1044439 Social stress in fathers affects sperm small RNA and offspring transcriptome profiles GSE248535 Transcriptome Analysis Environmental changes may affect paternal condition and following generations but the underlying mechanisms are poorly understood. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and development in their offspring. Here we investigated the role of sperm mediated small RNAs in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes and transposable elements TEs at this key developmental stage. We sequenced micro mi and Piwi interacting piRNAs in sperm and the full transcriptome in the embryos and ran differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from high to low competition environments. We identified 612 differentially expressed genes in the embryos. These results confirm that the social environment does not only affect males but also the molecular ecology of their sperm and the gene expression in their offspring suggesting a putative role of sRNAs. Overall design: This data deposit includes the small RNA seq data for this research. Male zebrafish were subject to high and low social stress environments with sperm samples collected RNA extracted and sent for small RNA sequencing.   pubmed:40121340   Hb 1 GSM7916504   tissue:sperm|cell line:sperm|genotype:WT|treatment:High2|geo loc name:missing|collection date:missing Hb 1 Reads were trimmed aligned with PatMan and then count matrices assembled before DESeq2 analyses Assembly: GRCz10 Supplementary files format and content: piRNA raw counts.csv raw counts of a subset of 24 out of the 40 samples that were analysed. These 24 were chosen due to noise observed in PCA clustering. Supplementary files format and content: mirna raw counts.csv raw counts for 39/40 samples as one sample failed to progress through our pipeline to the same high quality as the other 39 so this sample was omitted from analyses. sperm   Total RNA extraction New England BioLabs kit NEBNext®Multiplex Small RNA Library Prep Set for Illumina® Set 1 and 2 NEB #E7300 and NEB #E7580 small RNA library prep for Illumina   cell line:sperm|genotype:WT|treatment:High2 GSM7916504 GSM7916504: Hb 1; Danio rerio; ncRNA Seq GSM7916504 r1 GSM7916504 1 Total RNA extraction New England BioLabs kit NEBNext®Multiplex Small RNA Library Prep Set for Illumina® Set 1 and 2 NEB #E7300 and NEB #E7580 small RNA library prep for Illumina   ncRNA-Seq TRANSCRIPTOMIC size fractionation SINGLE ILLUMINA Illumina HiSeq 2500   SRP473892     12235X29_160303_D00294_0224_AC95NJANXX_7.cutadapt.se.19_100.fastq.gz fastq 595989272.0 21399963.0 GSM7916504 r1 0:27.85 A:128259525;C:125823294;G:181746588;T:160121380;N:38485 27       128259525 125823294 181746588 160121380 38485 SRX22630105 SRS19628587 SRA1756943 University of East Anglia University of East Anglia 1 0.85218   0.22941   0.83595   0.6151   30   B   usable mapping rate illumina hiseq_era unknown size_fractionation nebnext bulk unknown unknown   United Kingdom 2023-11-23 Undetermined Embryo Cell Line Cell Line
28989 28989 SRR26936234 SRX22630104 SRS19628586 SRP473892 PRJNA1044439 Social stress in fathers affects sperm small RNA and offspring transcriptome profiles GSE248535 Transcriptome Analysis Environmental changes may affect paternal condition and following generations but the underlying mechanisms are poorly understood. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and development in their offspring. Here we investigated the role of sperm mediated small RNAs in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes and transposable elements TEs at this key developmental stage. We sequenced micro mi and Piwi interacting piRNAs in sperm and the full transcriptome in the embryos and ran differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from high to low competition environments. We identified 612 differentially expressed genes in the embryos. These results confirm that the social environment does not only affect males but also the molecular ecology of their sperm and the gene expression in their offspring suggesting a putative role of sRNAs. Overall design: This data deposit includes the small RNA seq data for this research. Male zebrafish were subject to high and low social stress environments with sperm samples collected RNA extracted and sent for small RNA sequencing.   pubmed:40121340   Lb 6 GSM7916503   tissue:sperm|cell line:sperm|genotype:WT|treatment:Low2|geo loc name:missing|collection date:missing Lb 6 Reads were trimmed aligned with PatMan and then count matrices assembled before DESeq2 analyses Assembly: GRCz10 Supplementary files format and content: piRNA raw counts.csv raw counts of a subset of 24 out of the 40 samples that were analysed. These 24 were chosen due to noise observed in PCA clustering. Supplementary files format and content: mirna raw counts.csv raw counts for 39/40 samples as one sample failed to progress through our pipeline to the same high quality as the other 39 so this sample was omitted from analyses. sperm   Total RNA extraction New England BioLabs kit NEBNext®Multiplex Small RNA Library Prep Set for Illumina® Set 1 and 2 NEB #E7300 and NEB #E7580 small RNA library prep for Illumina   cell line:sperm|genotype:WT|treatment:Low2 GSM7916503 GSM7916503: Lb 6; Danio rerio; ncRNA Seq GSM7916503 r1 GSM7916503 1 Total RNA extraction New England BioLabs kit NEBNext®Multiplex Small RNA Library Prep Set for Illumina® Set 1 and 2 NEB #E7300 and NEB #E7580 small RNA library prep for Illumina   ncRNA-Seq TRANSCRIPTOMIC size fractionation SINGLE ILLUMINA Illumina HiSeq 2500   SRP473892     12235X28_160303_D00294_0224_AC95NJANXX_7.cutadapt.se.19_100.fastq.gz fastq 969307398.0 35511411.0 GSM7916503 r1 0:27.30 A:205174189;C:203840471;G:285952255;T:274278193;N:62290 27       205174189 203840471 285952255 274278193 62290 SRX22630104 SRS19628586 SRA1756943 University of East Anglia University of East Anglia 1 0.85414   0.22608   0.8505   0.62202   33   B   usable mapping rate illumina hiseq_era unknown size_fractionation nebnext bulk unknown unknown   United Kingdom 2023-11-23 Undetermined Embryo Cell Line Cell Line
28990 28990 SRR26936235 SRX22630103 SRS19628585 SRP473892 PRJNA1044439 Social stress in fathers affects sperm small RNA and offspring transcriptome profiles GSE248535 Transcriptome Analysis Environmental changes may affect paternal condition and following generations but the underlying mechanisms are poorly understood. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and development in their offspring. Here we investigated the role of sperm mediated small RNAs in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes and transposable elements TEs at this key developmental stage. We sequenced micro mi and Piwi interacting piRNAs in sperm and the full transcriptome in the embryos and ran differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from high to low competition environments. We identified 612 differentially expressed genes in the embryos. These results confirm that the social environment does not only affect males but also the molecular ecology of their sperm and the gene expression in their offspring suggesting a putative role of sRNAs. Overall design: This data deposit includes the small RNA seq data for this research. Male zebrafish were subject to high and low social stress environments with sperm samples collected RNA extracted and sent for small RNA sequencing.   pubmed:40121340   Ha 6 GSM7916502   tissue:sperm|cell line:sperm|genotype:WT|treatment:High1|geo loc name:missing|collection date:missing Ha 6 Reads were trimmed aligned with PatMan and then count matrices assembled before DESeq2 analyses Assembly: GRCz10 Supplementary files format and content: piRNA raw counts.csv raw counts of a subset of 24 out of the 40 samples that were analysed. These 24 were chosen due to noise observed in PCA clustering. Supplementary files format and content: mirna raw counts.csv raw counts for 39/40 samples as one sample failed to progress through our pipeline to the same high quality as the other 39 so this sample was omitted from analyses. sperm   Total RNA extraction New England BioLabs kit NEBNext®Multiplex Small RNA Library Prep Set for Illumina® Set 1 and 2 NEB #E7300 and NEB #E7580 small RNA library prep for Illumina   cell line:sperm|genotype:WT|treatment:High1 GSM7916502 GSM7916502: Ha 6; Danio rerio; ncRNA Seq GSM7916502 r1 GSM7916502 1 Total RNA extraction New England BioLabs kit NEBNext®Multiplex Small RNA Library Prep Set for Illumina® Set 1 and 2 NEB #E7300 and NEB #E7580 small RNA library prep for Illumina   ncRNA-Seq TRANSCRIPTOMIC size fractionation SINGLE ILLUMINA Illumina HiSeq 2500   SRP473892     12235X27_160303_D00294_0224_AC95NJANXX_7.cutadapt.se.19_100.fastq.gz fastq 1326612630.0 47094729.0 GSM7916502 r1 0:28.17 A:303455697;C:273680466;G:382978796;T:366416311;N:81360 28       303455697 273680466 382978796 366416311 81360 SRX22630103 SRS19628585 SRA1756943 University of East Anglia University of East Anglia 1 0.84786   0.24696   0.82946   0.68975   37   B   usable mapping rate illumina hiseq_era unknown size_fractionation nebnext bulk unknown unknown   United Kingdom 2023-11-23 Undetermined Embryo Cell Line Cell Line
28991 28991 SRR26936236 SRX22630102 SRS19628584 SRP473892 PRJNA1044439 Social stress in fathers affects sperm small RNA and offspring transcriptome profiles GSE248535 Transcriptome Analysis Environmental changes may affect paternal condition and following generations but the underlying mechanisms are poorly understood. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and development in their offspring. Here we investigated the role of sperm mediated small RNAs in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes and transposable elements TEs at this key developmental stage. We sequenced micro mi and Piwi interacting piRNAs in sperm and the full transcriptome in the embryos and ran differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from high to low competition environments. We identified 612 differentially expressed genes in the embryos. These results confirm that the social environment does not only affect males but also the molecular ecology of their sperm and the gene expression in their offspring suggesting a putative role of sRNAs. Overall design: This data deposit includes the small RNA seq data for this research. Male zebrafish were subject to high and low social stress environments with sperm samples collected RNA extracted and sent for small RNA sequencing.   pubmed:40121340   Ha 5 GSM7916501   tissue:sperm|cell line:sperm|genotype:WT|treatment:High1|geo loc name:missing|collection date:missing Ha 5 Reads were trimmed aligned with PatMan and then count matrices assembled before DESeq2 analyses Assembly: GRCz10 Supplementary files format and content: piRNA raw counts.csv raw counts of a subset of 24 out of the 40 samples that were analysed. These 24 were chosen due to noise observed in PCA clustering. Supplementary files format and content: mirna raw counts.csv raw counts for 39/40 samples as one sample failed to progress through our pipeline to the same high quality as the other 39 so this sample was omitted from analyses. sperm   Total RNA extraction New England BioLabs kit NEBNext®Multiplex Small RNA Library Prep Set for Illumina® Set 1 and 2 NEB #E7300 and NEB #E7580 small RNA library prep for Illumina   cell line:sperm|genotype:WT|treatment:High1 GSM7916501 GSM7916501: Ha 5; Danio rerio; ncRNA Seq GSM7916501 r1 GSM7916501 1 Total RNA extraction New England BioLabs kit NEBNext®Multiplex Small RNA Library Prep Set for Illumina® Set 1 and 2 NEB #E7300 and NEB #E7580 small RNA library prep for Illumina   ncRNA-Seq TRANSCRIPTOMIC size fractionation SINGLE ILLUMINA Illumina HiSeq 2500   SRP473892     12235X26_160303_D00294_0224_AC95NJANXX_7.cutadapt.se.19_100.fastq.gz fastq 471823428.0 16704799.0 GSM7916501 r1 0:28.24 A:103178860;C:100561292;G:155557190;T:112495106;N:30980 28       103178860 100561292 155557190 112495106 30980 SRX22630102 SRS19628584 SRA1756943 University of East Anglia University of East Anglia 1 0.61628   0.14433   0.91275   0.6543   28   B   usable mapping rate illumina hiseq_era unknown size_fractionation nebnext bulk unknown unknown   United Kingdom 2023-11-23 Undetermined Embryo Cell Line Cell Line
28992 28992 SRR26936237 SRX22630101 SRS19628583 SRP473892 PRJNA1044439 Social stress in fathers affects sperm small RNA and offspring transcriptome profiles GSE248535 Transcriptome Analysis Environmental changes may affect paternal condition and following generations but the underlying mechanisms are poorly understood. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and development in their offspring. Here we investigated the role of sperm mediated small RNAs in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes and transposable elements TEs at this key developmental stage. We sequenced micro mi and Piwi interacting piRNAs in sperm and the full transcriptome in the embryos and ran differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from high to low competition environments. We identified 612 differentially expressed genes in the embryos. These results confirm that the social environment does not only affect males but also the molecular ecology of their sperm and the gene expression in their offspring suggesting a putative role of sRNAs. Overall design: This data deposit includes the small RNA seq data for this research. Male zebrafish were subject to high and low social stress environments with sperm samples collected RNA extracted and sent for small RNA sequencing.   pubmed:40121340   La 4 GSM7916500   tissue:sperm|cell line:sperm|genotype:WT|treatment:Low1|geo loc name:missing|collection date:missing La 4 Reads were trimmed aligned with PatMan and then count matrices assembled before DESeq2 analyses Assembly: GRCz10 Supplementary files format and content: piRNA raw counts.csv raw counts of a subset of 24 out of the 40 samples that were analysed. These 24 were chosen due to noise observed in PCA clustering. Supplementary files format and content: mirna raw counts.csv raw counts for 39/40 samples as one sample failed to progress through our pipeline to the same high quality as the other 39 so this sample was omitted from analyses. sperm   Total RNA extraction New England BioLabs kit NEBNext®Multiplex Small RNA Library Prep Set for Illumina® Set 1 and 2 NEB #E7300 and NEB #E7580 small RNA library prep for Illumina   cell line:sperm|genotype:WT|treatment:Low1 GSM7916500 GSM7916500: La 4; Danio rerio; ncRNA Seq GSM7916500 r1 GSM7916500 1 Total RNA extraction New England BioLabs kit NEBNext®Multiplex Small RNA Library Prep Set for Illumina® Set 1 and 2 NEB #E7300 and NEB #E7580 small RNA library prep for Illumina   ncRNA-Seq TRANSCRIPTOMIC size fractionation SINGLE ILLUMINA Illumina HiSeq 2500   SRP473892     12235X25_160303_D00294_0224_AC95NJANXX_7.cutadapt.se.19_100.fastq.gz fastq 713371286.0 25212566.0 GSM7916500 r1 0:28.29 A:155201946;C:151864186;G:207971547;T:198288220;N:45387 28       155201946 151864186 207971547 198288220 45387 SRX22630101 SRS19628583 SRA1756943 University of East Anglia University of East Anglia 1 0.8507   0.2078   0.83031   0.62739   22   B   usable mapping rate illumina hiseq_era unknown size_fractionation nebnext bulk unknown unknown   United Kingdom 2023-11-23 Undetermined Embryo Cell Line Cell Line
28993 28993 SRR26936238 SRX22630100 SRS19628582 SRP473892 PRJNA1044439 Social stress in fathers affects sperm small RNA and offspring transcriptome profiles GSE248535 Transcriptome Analysis Environmental changes may affect paternal condition and following generations but the underlying mechanisms are poorly understood. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and development in their offspring. Here we investigated the role of sperm mediated small RNAs in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes and transposable elements TEs at this key developmental stage. We sequenced micro mi and Piwi interacting piRNAs in sperm and the full transcriptome in the embryos and ran differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from high to low competition environments. We identified 612 differentially expressed genes in the embryos. These results confirm that the social environment does not only affect males but also the molecular ecology of their sperm and the gene expression in their offspring suggesting a putative role of sRNAs. Overall design: This data deposit includes the small RNA seq data for this research. Male zebrafish were subject to high and low social stress environments with sperm samples collected RNA extracted and sent for small RNA sequencing.   pubmed:40121340   Hb 5 GSM7916499   tissue:sperm|cell line:sperm|genotype:WT|treatment:High2|geo loc name:missing|collection date:missing Hb 5 Reads were trimmed aligned with PatMan and then count matrices assembled before DESeq2 analyses Assembly: GRCz10 Supplementary files format and content: piRNA raw counts.csv raw counts of a subset of 24 out of the 40 samples that were analysed. These 24 were chosen due to noise observed in PCA clustering. Supplementary files format and content: mirna raw counts.csv raw counts for 39/40 samples as one sample failed to progress through our pipeline to the same high quality as the other 39 so this sample was omitted from analyses. sperm   Total RNA extraction New England BioLabs kit NEBNext®Multiplex Small RNA Library Prep Set for Illumina® Set 1 and 2 NEB #E7300 and NEB #E7580 small RNA library prep for Illumina   cell line:sperm|genotype:WT|treatment:High2 GSM7916499 GSM7916499: Hb 5; Danio rerio; ncRNA Seq GSM7916499 r1 GSM7916499 1 Total RNA extraction New England BioLabs kit NEBNext®Multiplex Small RNA Library Prep Set for Illumina® Set 1 and 2 NEB #E7300 and NEB #E7580 small RNA library prep for Illumina   ncRNA-Seq TRANSCRIPTOMIC size fractionation SINGLE ILLUMINA Illumina HiSeq 2500   SRP473892     12235X24_160303_D00294_0224_AC95NJANXX_6.cutadapt.se.19_100.fastq.gz fastq 1043060550.0 35761504.0 GSM7916499 r1 0:29.17 A:217942105;C:216398395;G:316456102;T:292217159;N:46789 29       217942105 216398395 316456102 292217159 46789 SRX22630100 SRS19628582 SRA1756943 University of East Anglia University of East Anglia 1 0.82114   0.20881   0.85622   0.68486   19   B   usable mapping rate illumina hiseq_era unknown size_fractionation nebnext bulk unknown unknown   United Kingdom 2023-11-23 Undetermined Embryo Cell Line Cell Line
28994 28994 SRR26936239 SRX22630099 SRS19628581 SRP473892 PRJNA1044439 Social stress in fathers affects sperm small RNA and offspring transcriptome profiles GSE248535 Transcriptome Analysis Environmental changes may affect paternal condition and following generations but the underlying mechanisms are poorly understood. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and development in their offspring. Here we investigated the role of sperm mediated small RNAs in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes and transposable elements TEs at this key developmental stage. We sequenced micro mi and Piwi interacting piRNAs in sperm and the full transcriptome in the embryos and ran differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from high to low competition environments. We identified 612 differentially expressed genes in the embryos. These results confirm that the social environment does not only affect males but also the molecular ecology of their sperm and the gene expression in their offspring suggesting a putative role of sRNAs. Overall design: This data deposit includes the small RNA seq data for this research. Male zebrafish were subject to high and low social stress environments with sperm samples collected RNA extracted and sent for small RNA sequencing.   pubmed:40121340   Hb 4 GSM7916498   tissue:sperm|cell line:sperm|genotype:WT|treatment:High2|geo loc name:missing|collection date:missing Hb 4 Reads were trimmed aligned with PatMan and then count matrices assembled before DESeq2 analyses Assembly: GRCz10 Supplementary files format and content: piRNA raw counts.csv raw counts of a subset of 24 out of the 40 samples that were analysed. These 24 were chosen due to noise observed in PCA clustering. Supplementary files format and content: mirna raw counts.csv raw counts for 39/40 samples as one sample failed to progress through our pipeline to the same high quality as the other 39 so this sample was omitted from analyses. sperm   Total RNA extraction New England BioLabs kit NEBNext®Multiplex Small RNA Library Prep Set for Illumina® Set 1 and 2 NEB #E7300 and NEB #E7580 small RNA library prep for Illumina   cell line:sperm|genotype:WT|treatment:High2 GSM7916498 GSM7916498: Hb 4; Danio rerio; ncRNA Seq GSM7916498 r1 GSM7916498 1 Total RNA extraction New England BioLabs kit NEBNext®Multiplex Small RNA Library Prep Set for Illumina® Set 1 and 2 NEB #E7300 and NEB #E7580 small RNA library prep for Illumina   ncRNA-Seq TRANSCRIPTOMIC size fractionation SINGLE ILLUMINA Illumina HiSeq 2500   SRP473892     12235X23_160303_D00294_0224_AC95NJANXX_6.cutadapt.se.19_100.fastq.gz fastq 1357956641.0 47869361.0 GSM7916498 r1 0:28.37 A:293740473;C:276333777;G:404639503;T:383181225;N:61663 28       293740473 276333777 404639503 383181225 61663 SRX22630099 SRS19628581 SRA1756943 University of East Anglia University of East Anglia 1 0.79885   0.22306   0.8425   0.6656   26   B   usable mapping rate illumina hiseq_era unknown size_fractionation nebnext bulk unknown unknown   United Kingdom 2023-11-23 Undetermined Embryo Cell Line Cell Line
28995 28995 SRR26936240 SRX22630098 SRS19628580 SRP473892 PRJNA1044439 Social stress in fathers affects sperm small RNA and offspring transcriptome profiles GSE248535 Transcriptome Analysis Environmental changes may affect paternal condition and following generations but the underlying mechanisms are poorly understood. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and development in their offspring. Here we investigated the role of sperm mediated small RNAs in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes and transposable elements TEs at this key developmental stage. We sequenced micro mi and Piwi interacting piRNAs in sperm and the full transcriptome in the embryos and ran differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from high to low competition environments. We identified 612 differentially expressed genes in the embryos. These results confirm that the social environment does not only affect males but also the molecular ecology of their sperm and the gene expression in their offspring suggesting a putative role of sRNAs. Overall design: This data deposit includes the small RNA seq data for this research. Male zebrafish were subject to high and low social stress environments with sperm samples collected RNA extracted and sent for small RNA sequencing.   pubmed:40121340   Lb 5 GSM7916497   tissue:sperm|cell line:sperm|genotype:WT|treatment:Low2|geo loc name:missing|collection date:missing Lb 5 Reads were trimmed aligned with PatMan and then count matrices assembled before DESeq2 analyses Assembly: GRCz10 Supplementary files format and content: piRNA raw counts.csv raw counts of a subset of 24 out of the 40 samples that were analysed. These 24 were chosen due to noise observed in PCA clustering. Supplementary files format and content: mirna raw counts.csv raw counts for 39/40 samples as one sample failed to progress through our pipeline to the same high quality as the other 39 so this sample was omitted from analyses. sperm   Total RNA extraction New England BioLabs kit NEBNext®Multiplex Small RNA Library Prep Set for Illumina® Set 1 and 2 NEB #E7300 and NEB #E7580 small RNA library prep for Illumina   cell line:sperm|genotype:WT|treatment:Low2 GSM7916497 GSM7916497: Lb 5; Danio rerio; ncRNA Seq GSM7916497 r1 GSM7916497 1 Total RNA extraction New England BioLabs kit NEBNext®Multiplex Small RNA Library Prep Set for Illumina® Set 1 and 2 NEB #E7300 and NEB #E7580 small RNA library prep for Illumina   ncRNA-Seq TRANSCRIPTOMIC size fractionation SINGLE ILLUMINA Illumina HiSeq 2500   SRP473892     12235X22_160303_D00294_0224_AC95NJANXX_6.cutadapt.se.19_100.fastq.gz fastq 669771811.0 23848259.0 GSM7916497 r1 0:28.08 A:132916986;C:135594797;G:198730347;T:202498953;N:30728 28       132916986 135594797 198730347 202498953 30728 SRX22630098 SRS19628580 SRA1756943 University of East Anglia University of East Anglia 1 0.80999   0.24188   0.84658   0.68339   20   B   usable mapping rate illumina hiseq_era unknown size_fractionation nebnext bulk unknown unknown   United Kingdom 2023-11-23 Undetermined Embryo Cell Line Cell Line
28996 28996 SRR26936241 SRX22630097 SRS19628579 SRP473892 PRJNA1044439 Social stress in fathers affects sperm small RNA and offspring transcriptome profiles GSE248535 Transcriptome Analysis Environmental changes may affect paternal condition and following generations but the underlying mechanisms are poorly understood. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and development in their offspring. Here we investigated the role of sperm mediated small RNAs in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes and transposable elements TEs at this key developmental stage. We sequenced micro mi and Piwi interacting piRNAs in sperm and the full transcriptome in the embryos and ran differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from high to low competition environments. We identified 612 differentially expressed genes in the embryos. These results confirm that the social environment does not only affect males but also the molecular ecology of their sperm and the gene expression in their offspring suggesting a putative role of sRNAs. Overall design: This data deposit includes the small RNA seq data for this research. Male zebrafish were subject to high and low social stress environments with sperm samples collected RNA extracted and sent for small RNA sequencing.   pubmed:40121340   Ha 4 GSM7916496   tissue:sperm|cell line:sperm|genotype:WT|treatment:High1|geo loc name:missing|collection date:missing Ha 4 Reads were trimmed aligned with PatMan and then count matrices assembled before DESeq2 analyses Assembly: GRCz10 Supplementary files format and content: piRNA raw counts.csv raw counts of a subset of 24 out of the 40 samples that were analysed. These 24 were chosen due to noise observed in PCA clustering. Supplementary files format and content: mirna raw counts.csv raw counts for 39/40 samples as one sample failed to progress through our pipeline to the same high quality as the other 39 so this sample was omitted from analyses. sperm   Total RNA extraction New England BioLabs kit NEBNext®Multiplex Small RNA Library Prep Set for Illumina® Set 1 and 2 NEB #E7300 and NEB #E7580 small RNA library prep for Illumina   cell line:sperm|genotype:WT|treatment:High1 GSM7916496 GSM7916496: Ha 4; Danio rerio; ncRNA Seq GSM7916496 r1 GSM7916496 1 Total RNA extraction New England BioLabs kit NEBNext®Multiplex Small RNA Library Prep Set for Illumina® Set 1 and 2 NEB #E7300 and NEB #E7580 small RNA library prep for Illumina   ncRNA-Seq TRANSCRIPTOMIC size fractionation SINGLE ILLUMINA Illumina HiSeq 2500   SRP473892     12235X21_160303_D00294_0224_AC95NJANXX_6.cutadapt.se.19_100.fastq.gz fastq 853803123.0 28538725.0 GSM7916496 r1 0:29.92 A:158220512;C:186146914;G:264519321;T:244878221;N:38155 29       158220512 186146914 264519321 244878221 38155 SRX22630097 SRS19628579 SRA1756943 University of East Anglia University of East Anglia 1 0.828   0.18458   0.85851   0.6483   32   B   usable mapping rate illumina hiseq_era unknown size_fractionation nebnext bulk unknown unknown   United Kingdom 2023-11-23 Undetermined Embryo Cell Line Cell Line
28997 28997 SRR26936242 SRX22630096 SRS19628578 SRP473892 PRJNA1044439 Social stress in fathers affects sperm small RNA and offspring transcriptome profiles GSE248535 Transcriptome Analysis Environmental changes may affect paternal condition and following generations but the underlying mechanisms are poorly understood. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and development in their offspring. Here we investigated the role of sperm mediated small RNAs in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes and transposable elements TEs at this key developmental stage. We sequenced micro mi and Piwi interacting piRNAs in sperm and the full transcriptome in the embryos and ran differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from high to low competition environments. We identified 612 differentially expressed genes in the embryos. These results confirm that the social environment does not only affect males but also the molecular ecology of their sperm and the gene expression in their offspring suggesting a putative role of sRNAs. Overall design: This data deposit includes the small RNA seq data for this research. Male zebrafish were subject to high and low social stress environments with sperm samples collected RNA extracted and sent for small RNA sequencing.   pubmed:40121340   Ha 3 GSM7916495   tissue:sperm|cell line:sperm|genotype:WT|treatment:High1|geo loc name:missing|collection date:missing Ha 3 Reads were trimmed aligned with PatMan and then count matrices assembled before DESeq2 analyses Assembly: GRCz10 Supplementary files format and content: piRNA raw counts.csv raw counts of a subset of 24 out of the 40 samples that were analysed. These 24 were chosen due to noise observed in PCA clustering. Supplementary files format and content: mirna raw counts.csv raw counts for 39/40 samples as one sample failed to progress through our pipeline to the same high quality as the other 39 so this sample was omitted from analyses. sperm   Total RNA extraction New England BioLabs kit NEBNext®Multiplex Small RNA Library Prep Set for Illumina® Set 1 and 2 NEB #E7300 and NEB #E7580 small RNA library prep for Illumina   cell line:sperm|genotype:WT|treatment:High1 GSM7916495 GSM7916495: Ha 3; Danio rerio; ncRNA Seq GSM7916495 r1 GSM7916495 1 Total RNA extraction New England BioLabs kit NEBNext®Multiplex Small RNA Library Prep Set for Illumina® Set 1 and 2 NEB #E7300 and NEB #E7580 small RNA library prep for Illumina   ncRNA-Seq TRANSCRIPTOMIC size fractionation SINGLE ILLUMINA Illumina HiSeq 2500   SRP473892     12235X20_160303_D00294_0224_AC95NJANXX_6.cutadapt.se.19_100.fastq.gz fastq 629106097.0 21383957.0 GSM7916495 r1 0:29.42 A:117216944;C:131994022;G:192623524;T:187243523;N:28084 29       117216944 131994022 192623524 187243523 28084 SRX22630096 SRS19628578 SRA1756943 University of East Anglia University of East Anglia 1 0.8166   0.20457   0.84112   0.65357   22   B   usable mapping rate illumina hiseq_era unknown size_fractionation nebnext bulk unknown unknown   United Kingdom 2023-11-23 Undetermined Embryo Cell Line Cell Line
28998 28998 SRR26936243 SRX22630095 SRS19628577 SRP473892 PRJNA1044439 Social stress in fathers affects sperm small RNA and offspring transcriptome profiles GSE248535 Transcriptome Analysis Environmental changes may affect paternal condition and following generations but the underlying mechanisms are poorly understood. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and development in their offspring. Here we investigated the role of sperm mediated small RNAs in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes and transposable elements TEs at this key developmental stage. We sequenced micro mi and Piwi interacting piRNAs in sperm and the full transcriptome in the embryos and ran differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from high to low competition environments. We identified 612 differentially expressed genes in the embryos. These results confirm that the social environment does not only affect males but also the molecular ecology of their sperm and the gene expression in their offspring suggesting a putative role of sRNAs. Overall design: This data deposit includes the small RNA seq data for this research. Male zebrafish were subject to high and low social stress environments with sperm samples collected RNA extracted and sent for small RNA sequencing.   pubmed:40121340   La 1 GSM7916494   tissue:sperm|cell line:sperm|genotype:WT|treatment:Low1|geo loc name:missing|collection date:missing La 1 Reads were trimmed aligned with PatMan and then count matrices assembled before DESeq2 analyses Assembly: GRCz10 Supplementary files format and content: piRNA raw counts.csv raw counts of a subset of 24 out of the 40 samples that were analysed. These 24 were chosen due to noise observed in PCA clustering. Supplementary files format and content: mirna raw counts.csv raw counts for 39/40 samples as one sample failed to progress through our pipeline to the same high quality as the other 39 so this sample was omitted from analyses. sperm   Total RNA extraction New England BioLabs kit NEBNext®Multiplex Small RNA Library Prep Set for Illumina® Set 1 and 2 NEB #E7300 and NEB #E7580 small RNA library prep for Illumina   cell line:sperm|genotype:WT|treatment:Low1 GSM7916494 GSM7916494: La 1; Danio rerio; ncRNA Seq GSM7916494 r1 GSM7916494 1 Total RNA extraction New England BioLabs kit NEBNext®Multiplex Small RNA Library Prep Set for Illumina® Set 1 and 2 NEB #E7300 and NEB #E7580 small RNA library prep for Illumina   ncRNA-Seq TRANSCRIPTOMIC size fractionation SINGLE ILLUMINA Illumina HiSeq 2500   SRP473892     12235X19_160303_D00294_0224_AC95NJANXX_6.cutadapt.se.19_100.fastq.gz fastq 21178378.0 739280.0 GSM7916494 r1 0:28.65 A:4323144;C:4478932;G:6303136;T:6072190;N:976 28       4323144 4478932 6303136 6072190 976 SRX22630095 SRS19628577 SRA1756943 University of East Anglia University of East Anglia 1 0.85277   0.21158   0.87434   0.62601   26   B   usable mapping rate illumina hiseq_era unknown size_fractionation nebnext bulk unknown unknown   United Kingdom 2023-11-23 Undetermined Embryo Cell Line Cell Line
28999 28999 SRR26936244 SRX22630094 SRS19628576 SRP473892 PRJNA1044439 Social stress in fathers affects sperm small RNA and offspring transcriptome profiles GSE248535 Transcriptome Analysis Environmental changes may affect paternal condition and following generations but the underlying mechanisms are poorly understood. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and development in their offspring. Here we investigated the role of sperm mediated small RNAs in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes and transposable elements TEs at this key developmental stage. We sequenced micro mi and Piwi interacting piRNAs in sperm and the full transcriptome in the embryos and ran differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from high to low competition environments. We identified 612 differentially expressed genes in the embryos. These results confirm that the social environment does not only affect males but also the molecular ecology of their sperm and the gene expression in their offspring suggesting a putative role of sRNAs. Overall design: This data deposit includes the small RNA seq data for this research. Male zebrafish were subject to high and low social stress environments with sperm samples collected RNA extracted and sent for small RNA sequencing.   pubmed:40121340   Lb 4 GSM7916493   tissue:sperm|cell line:sperm|genotype:WT|treatment:Low2|geo loc name:missing|collection date:missing Lb 4 Reads were trimmed aligned with PatMan and then count matrices assembled before DESeq2 analyses Assembly: GRCz10 Supplementary files format and content: piRNA raw counts.csv raw counts of a subset of 24 out of the 40 samples that were analysed. These 24 were chosen due to noise observed in PCA clustering. Supplementary files format and content: mirna raw counts.csv raw counts for 39/40 samples as one sample failed to progress through our pipeline to the same high quality as the other 39 so this sample was omitted from analyses. sperm   Total RNA extraction New England BioLabs kit NEBNext®Multiplex Small RNA Library Prep Set for Illumina® Set 1 and 2 NEB #E7300 and NEB #E7580 small RNA library prep for Illumina   cell line:sperm|genotype:WT|treatment:Low2 GSM7916493 GSM7916493: Lb 4; Danio rerio; ncRNA Seq GSM7916493 r1 GSM7916493 1 Total RNA extraction New England BioLabs kit NEBNext®Multiplex Small RNA Library Prep Set for Illumina® Set 1 and 2 NEB #E7300 and NEB #E7580 small RNA library prep for Illumina   ncRNA-Seq TRANSCRIPTOMIC size fractionation SINGLE ILLUMINA Illumina HiSeq 2500   SRP473892     12235X18_160303_D00294_0224_AC95NJANXX_6.cutadapt.se.19_100.fastq.gz fastq 959247745.0 33043430.0 GSM7916493 r1 0:29.03 A:225208425;C:212535680;G:284062577;T:237397072;N:43991 29       225208425 212535680 284062577 237397072 43991 SRX22630094 SRS19628576 SRA1756943 University of East Anglia University of East Anglia 1 0.82232   0.22592   0.87073   0.6135   22   B   usable mapping rate illumina hiseq_era unknown size_fractionation nebnext bulk unknown unknown   United Kingdom 2023-11-23 Undetermined Embryo Cell Line Cell Line
29000 29000 SRR26936245 SRX22630093 SRS19628575 SRP473892 PRJNA1044439 Social stress in fathers affects sperm small RNA and offspring transcriptome profiles GSE248535 Transcriptome Analysis Environmental changes may affect paternal condition and following generations but the underlying mechanisms are poorly understood. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and development in their offspring. Here we investigated the role of sperm mediated small RNAs in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes and transposable elements TEs at this key developmental stage. We sequenced micro mi and Piwi interacting piRNAs in sperm and the full transcriptome in the embryos and ran differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from high to low competition environments. We identified 612 differentially expressed genes in the embryos. These results confirm that the social environment does not only affect males but also the molecular ecology of their sperm and the gene expression in their offspring suggesting a putative role of sRNAs. Overall design: This data deposit includes the small RNA seq data for this research. Male zebrafish were subject to high and low social stress environments with sperm samples collected RNA extracted and sent for small RNA sequencing.   pubmed:40121340   Lb 3 GSM7916492   tissue:sperm|cell line:sperm|genotype:WT|treatment:Low1|geo loc name:missing|collection date:missing Lb 3 Reads were trimmed aligned with PatMan and then count matrices assembled before DESeq2 analyses Assembly: GRCz10 Supplementary files format and content: piRNA raw counts.csv raw counts of a subset of 24 out of the 40 samples that were analysed. These 24 were chosen due to noise observed in PCA clustering. Supplementary files format and content: mirna raw counts.csv raw counts for 39/40 samples as one sample failed to progress through our pipeline to the same high quality as the other 39 so this sample was omitted from analyses. sperm   Total RNA extraction New England BioLabs kit NEBNext®Multiplex Small RNA Library Prep Set for Illumina® Set 1 and 2 NEB #E7300 and NEB #E7580 small RNA library prep for Illumina   cell line:sperm|genotype:WT|treatment:Low1 GSM7916492 GSM7916492: Lb 3; Danio rerio; ncRNA Seq GSM7916492 r1 GSM7916492 1 Total RNA extraction New England BioLabs kit NEBNext®Multiplex Small RNA Library Prep Set for Illumina® Set 1 and 2 NEB #E7300 and NEB #E7580 small RNA library prep for Illumina   ncRNA-Seq TRANSCRIPTOMIC size fractionation SINGLE ILLUMINA Illumina HiSeq 2500   SRP473892     12235X17_160303_D00294_0224_AC95NJANXX_6.cutadapt.se.19_100.fastq.gz fastq 900287704.0 31947371.0 GSM7916492 r1 0:28.18 A:224093089;C:188919051;G:262824615;T:224407166;N:43783 28       224093089 188919051 262824615 224407166 43783 SRX22630093 SRS19628575 SRA1756943 University of East Anglia University of East Anglia 1 0.67003   0.17024   0.87095   0.6869   19   B   usable mapping rate illumina hiseq_era unknown size_fractionation nebnext bulk unknown unknown   United Kingdom 2023-11-23 Undetermined Embryo Cell Line Cell Line
29001 29001 SRR26936246 SRX22630092 SRS19628574 SRP473892 PRJNA1044439 Social stress in fathers affects sperm small RNA and offspring transcriptome profiles GSE248535 Transcriptome Analysis Environmental changes may affect paternal condition and following generations but the underlying mechanisms are poorly understood. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and development in their offspring. Here we investigated the role of sperm mediated small RNAs in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes and transposable elements TEs at this key developmental stage. We sequenced micro mi and Piwi interacting piRNAs in sperm and the full transcriptome in the embryos and ran differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from high to low competition environments. We identified 612 differentially expressed genes in the embryos. These results confirm that the social environment does not only affect males but also the molecular ecology of their sperm and the gene expression in their offspring suggesting a putative role of sRNAs. Overall design: This data deposit includes the small RNA seq data for this research. Male zebrafish were subject to high and low social stress environments with sperm samples collected RNA extracted and sent for small RNA sequencing.   pubmed:40121340   Hb 3 GSM7916491   tissue:sperm|cell line:sperm|genotype:WT|treatment:Low2|geo loc name:missing|collection date:missing Hb 3 Reads were trimmed aligned with PatMan and then count matrices assembled before DESeq2 analyses Assembly: GRCz10 Supplementary files format and content: piRNA raw counts.csv raw counts of a subset of 24 out of the 40 samples that were analysed. These 24 were chosen due to noise observed in PCA clustering. Supplementary files format and content: mirna raw counts.csv raw counts for 39/40 samples as one sample failed to progress through our pipeline to the same high quality as the other 39 so this sample was omitted from analyses. sperm   Total RNA extraction New England BioLabs kit NEBNext®Multiplex Small RNA Library Prep Set for Illumina® Set 1 and 2 NEB #E7300 and NEB #E7580 small RNA library prep for Illumina   cell line:sperm|genotype:WT|treatment:Low2 GSM7916491 GSM7916491: Hb 3; Danio rerio; ncRNA Seq GSM7916491 r1 GSM7916491 1 Total RNA extraction New England BioLabs kit NEBNext®Multiplex Small RNA Library Prep Set for Illumina® Set 1 and 2 NEB #E7300 and NEB #E7580 small RNA library prep for Illumina   ncRNA-Seq TRANSCRIPTOMIC size fractionation SINGLE ILLUMINA Illumina HiSeq 2500   SRP473892     12235X16_160303_D00294_0224_AC95NJANXX_5.cutadapt.se.19_100.fastq.gz fastq 80986783.0 3427704.0 GSM7916491 r1 0:23.63 A:18164838;C:19014879;G:24153410;T:19641723;N:11933 23       18164838 19014879 24153410 19641723 11933 SRX22630092 SRS19628574 SRA1756943 University of East Anglia University of East Anglia 1 0.83312   0.24402   0.88749   0.63345   50   B   usable mapping rate illumina hiseq_era unknown size_fractionation nebnext bulk unknown unknown   United Kingdom 2023-11-23 Undetermined Embryo Cell Line Cell Line
29002 29002 SRR26936247 SRX22630091 SRS19628573 SRP473892 PRJNA1044439 Social stress in fathers affects sperm small RNA and offspring transcriptome profiles GSE248535 Transcriptome Analysis Environmental changes may affect paternal condition and following generations but the underlying mechanisms are poorly understood. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and development in their offspring. Here we investigated the role of sperm mediated small RNAs in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes and transposable elements TEs at this key developmental stage. We sequenced micro mi and Piwi interacting piRNAs in sperm and the full transcriptome in the embryos and ran differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from high to low competition environments. We identified 612 differentially expressed genes in the embryos. These results confirm that the social environment does not only affect males but also the molecular ecology of their sperm and the gene expression in their offspring suggesting a putative role of sRNAs. Overall design: This data deposit includes the small RNA seq data for this research. Male zebrafish were subject to high and low social stress environments with sperm samples collected RNA extracted and sent for small RNA sequencing.   pubmed:40121340   La 3 GSM7916490   tissue:sperm|cell line:sperm|genotype:WT|treatment:High2|geo loc name:missing|collection date:missing La 3 Reads were trimmed aligned with PatMan and then count matrices assembled before DESeq2 analyses Assembly: GRCz10 Supplementary files format and content: piRNA raw counts.csv raw counts of a subset of 24 out of the 40 samples that were analysed. These 24 were chosen due to noise observed in PCA clustering. Supplementary files format and content: mirna raw counts.csv raw counts for 39/40 samples as one sample failed to progress through our pipeline to the same high quality as the other 39 so this sample was omitted from analyses. sperm   Total RNA extraction New England BioLabs kit NEBNext®Multiplex Small RNA Library Prep Set for Illumina® Set 1 and 2 NEB #E7300 and NEB #E7580 small RNA library prep for Illumina   cell line:sperm|genotype:WT|treatment:High2 GSM7916490 GSM7916490: La 3; Danio rerio; ncRNA Seq GSM7916490 r1 GSM7916490 1 Total RNA extraction New England BioLabs kit NEBNext®Multiplex Small RNA Library Prep Set for Illumina® Set 1 and 2 NEB #E7300 and NEB #E7580 small RNA library prep for Illumina   ncRNA-Seq TRANSCRIPTOMIC size fractionation SINGLE ILLUMINA Illumina HiSeq 2500   SRP473892     12235X15_160303_D00294_0224_AC95NJANXX_5.cutadapt.se.19_100.fastq.gz fastq 148933985.0 6314805.0 GSM7916490 r1 0:23.58 A:39058546;C:30363738;G:44242466;T:35240575;N:28660 23       39058546 30363738 44242466 35240575 28660 SRX22630091 SRS19628573 SRA1756943 University of East Anglia University of East Anglia 1 0.41302   0.13369   0.89802   0.57553   22   B   usable mapping rate illumina hiseq_era unknown size_fractionation nebnext bulk unknown unknown   United Kingdom 2023-11-23 Undetermined Embryo Cell Line Cell Line
29003 29003 SRR26936248 SRX22630090 SRS19628572 SRP473892 PRJNA1044439 Social stress in fathers affects sperm small RNA and offspring transcriptome profiles GSE248535 Transcriptome Analysis Environmental changes may affect paternal condition and following generations but the underlying mechanisms are poorly understood. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and development in their offspring. Here we investigated the role of sperm mediated small RNAs in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes and transposable elements TEs at this key developmental stage. We sequenced micro mi and Piwi interacting piRNAs in sperm and the full transcriptome in the embryos and ran differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from high to low competition environments. We identified 612 differentially expressed genes in the embryos. These results confirm that the social environment does not only affect males but also the molecular ecology of their sperm and the gene expression in their offspring suggesting a putative role of sRNAs. Overall design: This data deposit includes the small RNA seq data for this research. Male zebrafish were subject to high and low social stress environments with sperm samples collected RNA extracted and sent for small RNA sequencing.   pubmed:40121340   La 2 GSM7916489   tissue:sperm|cell line:sperm|genotype:WT|treatment:Low1|geo loc name:missing|collection date:missing La 2 Reads were trimmed aligned with PatMan and then count matrices assembled before DESeq2 analyses Assembly: GRCz10 Supplementary files format and content: piRNA raw counts.csv raw counts of a subset of 24 out of the 40 samples that were analysed. These 24 were chosen due to noise observed in PCA clustering. Supplementary files format and content: mirna raw counts.csv raw counts for 39/40 samples as one sample failed to progress through our pipeline to the same high quality as the other 39 so this sample was omitted from analyses. sperm   Total RNA extraction New England BioLabs kit NEBNext®Multiplex Small RNA Library Prep Set for Illumina® Set 1 and 2 NEB #E7300 and NEB #E7580 small RNA library prep for Illumina   cell line:sperm|genotype:WT|treatment:Low1 GSM7916489 GSM7916489: La 2; Danio rerio; ncRNA Seq GSM7916489 r1 GSM7916489 1 Total RNA extraction New England BioLabs kit NEBNext®Multiplex Small RNA Library Prep Set for Illumina® Set 1 and 2 NEB #E7300 and NEB #E7580 small RNA library prep for Illumina   ncRNA-Seq TRANSCRIPTOMIC size fractionation SINGLE ILLUMINA Illumina HiSeq 2500   SRP473892     12235X14_160303_D00294_0224_AC95NJANXX_5.cutadapt.se.19_100.fastq.gz fastq 538382421.0 23079847.0 GSM7916489 r1 0:23.33 A:119659971;C:112896468;G:162693210;T:143063074;N:69698 23       119659971 112896468 162693210 143063074 69698 SRX22630090 SRS19628572 SRA1756943 University of East Anglia University of East Anglia 1 0.81532   0.23945   0.8758   0.62425   50   B   usable mapping rate illumina hiseq_era unknown size_fractionation nebnext bulk unknown unknown   United Kingdom 2023-11-23 Undetermined Embryo Cell Line Cell Line
29004 29004 SRR26936249 SRX22630089 SRS19628571 SRP473892 PRJNA1044439 Social stress in fathers affects sperm small RNA and offspring transcriptome profiles GSE248535 Transcriptome Analysis Environmental changes may affect paternal condition and following generations but the underlying mechanisms are poorly understood. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and development in their offspring. Here we investigated the role of sperm mediated small RNAs in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes and transposable elements TEs at this key developmental stage. We sequenced micro mi and Piwi interacting piRNAs in sperm and the full transcriptome in the embryos and ran differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from high to low competition environments. We identified 612 differentially expressed genes in the embryos. These results confirm that the social environment does not only affect males but also the molecular ecology of their sperm and the gene expression in their offspring suggesting a putative role of sRNAs. Overall design: This data deposit includes the small RNA seq data for this research. Male zebrafish were subject to high and low social stress environments with sperm samples collected RNA extracted and sent for small RNA sequencing.   pubmed:40121340   Ha 1 GSM7916488   tissue:sperm|cell line:sperm|genotype:WT|treatment:High1|geo loc name:missing|collection date:missing Ha 1 Reads were trimmed aligned with PatMan and then count matrices assembled before DESeq2 analyses Assembly: GRCz10 Supplementary files format and content: piRNA raw counts.csv raw counts of a subset of 24 out of the 40 samples that were analysed. These 24 were chosen due to noise observed in PCA clustering. Supplementary files format and content: mirna raw counts.csv raw counts for 39/40 samples as one sample failed to progress through our pipeline to the same high quality as the other 39 so this sample was omitted from analyses. sperm   Total RNA extraction New England BioLabs kit NEBNext®Multiplex Small RNA Library Prep Set for Illumina® Set 1 and 2 NEB #E7300 and NEB #E7580 small RNA library prep for Illumina   cell line:sperm|genotype:WT|treatment:High1 GSM7916488 GSM7916488: Ha 1; Danio rerio; ncRNA Seq GSM7916488 r1 GSM7916488 1 Total RNA extraction New England BioLabs kit NEBNext®Multiplex Small RNA Library Prep Set for Illumina® Set 1 and 2 NEB #E7300 and NEB #E7580 small RNA library prep for Illumina   ncRNA-Seq TRANSCRIPTOMIC size fractionation SINGLE ILLUMINA Illumina HiSeq 2500   SRP473892     12235X13_160303_D00294_0224_AC95NJANXX_5.cutadapt.se.19_100.fastq.gz fastq 761541737.0 32310434.0 GSM7916488 r1 0:23.57 A:182485471;C:173949932;G:214306916;T:190683160;N:116258 23       182485471 173949932 214306916 190683160 116258 SRX22630089 SRS19628571 SRA1756943 University of East Anglia University of East Anglia 1 0.82936   0.23022   0.85098   0.59571   19   B   usable mapping rate illumina hiseq_era unknown size_fractionation nebnext bulk unknown unknown   United Kingdom 2023-11-23 Undetermined Embryo Cell Line Cell Line
29005 29005 SRR26936250 SRX22630088 SRS19628570 SRP473892 PRJNA1044439 Social stress in fathers affects sperm small RNA and offspring transcriptome profiles GSE248535 Transcriptome Analysis Environmental changes may affect paternal condition and following generations but the underlying mechanisms are poorly understood. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and development in their offspring. Here we investigated the role of sperm mediated small RNAs in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes and transposable elements TEs at this key developmental stage. We sequenced micro mi and Piwi interacting piRNAs in sperm and the full transcriptome in the embryos and ran differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from high to low competition environments. We identified 612 differentially expressed genes in the embryos. These results confirm that the social environment does not only affect males but also the molecular ecology of their sperm and the gene expression in their offspring suggesting a putative role of sRNAs. Overall design: This data deposit includes the small RNA seq data for this research. Male zebrafish were subject to high and low social stress environments with sperm samples collected RNA extracted and sent for small RNA sequencing.   pubmed:40121340   Lb 2 GSM7916487   tissue:sperm|cell line:sperm|genotype:WT|treatment:Low2|geo loc name:missing|collection date:missing Lb 2 Reads were trimmed aligned with PatMan and then count matrices assembled before DESeq2 analyses Assembly: GRCz10 Supplementary files format and content: piRNA raw counts.csv raw counts of a subset of 24 out of the 40 samples that were analysed. These 24 were chosen due to noise observed in PCA clustering. Supplementary files format and content: mirna raw counts.csv raw counts for 39/40 samples as one sample failed to progress through our pipeline to the same high quality as the other 39 so this sample was omitted from analyses. sperm   Total RNA extraction New England BioLabs kit NEBNext®Multiplex Small RNA Library Prep Set for Illumina® Set 1 and 2 NEB #E7300 and NEB #E7580 small RNA library prep for Illumina   cell line:sperm|genotype:WT|treatment:Low2 GSM7916487 GSM7916487: Lb 2; Danio rerio; ncRNA Seq GSM7916487 r1 GSM7916487 1 Total RNA extraction New England BioLabs kit NEBNext®Multiplex Small RNA Library Prep Set for Illumina® Set 1 and 2 NEB #E7300 and NEB #E7580 small RNA library prep for Illumina   ncRNA-Seq TRANSCRIPTOMIC size fractionation SINGLE ILLUMINA Illumina HiSeq 2500   SRP473892     12235X12_160303_D00294_0224_AC95NJANXX_5.cutadapt.se.19_100.fastq.gz fastq 559417322.0 23757934.0 GSM7916487 r1 0:23.55 A:126286771;C:106333997;G:170445137;T:156287131;N:64286 23       126286771 106333997 170445137 156287131 64286 SRX22630088 SRS19628570 SRA1756943 University of East Anglia University of East Anglia 1 0.85098   0.26224   0.8608   0.59344   22   B   usable mapping rate illumina hiseq_era unknown size_fractionation nebnext bulk unknown unknown   United Kingdom 2023-11-23 Undetermined Embryo Cell Line Cell Line
29006 29006 SRR26936251 SRX22630087 SRS19628569 SRP473892 PRJNA1044439 Social stress in fathers affects sperm small RNA and offspring transcriptome profiles GSE248535 Transcriptome Analysis Environmental changes may affect paternal condition and following generations but the underlying mechanisms are poorly understood. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and development in their offspring. Here we investigated the role of sperm mediated small RNAs in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes and transposable elements TEs at this key developmental stage. We sequenced micro mi and Piwi interacting piRNAs in sperm and the full transcriptome in the embryos and ran differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from high to low competition environments. We identified 612 differentially expressed genes in the embryos. These results confirm that the social environment does not only affect males but also the molecular ecology of their sperm and the gene expression in their offspring suggesting a putative role of sRNAs. Overall design: This data deposit includes the small RNA seq data for this research. Male zebrafish were subject to high and low social stress environments with sperm samples collected RNA extracted and sent for small RNA sequencing.   pubmed:40121340   Lb 1 GSM7916486   tissue:sperm|cell line:sperm|genotype:WT|treatment:High2|geo loc name:missing|collection date:missing Lb 1 Reads were trimmed aligned with PatMan and then count matrices assembled before DESeq2 analyses Assembly: GRCz10 Supplementary files format and content: piRNA raw counts.csv raw counts of a subset of 24 out of the 40 samples that were analysed. These 24 were chosen due to noise observed in PCA clustering. Supplementary files format and content: mirna raw counts.csv raw counts for 39/40 samples as one sample failed to progress through our pipeline to the same high quality as the other 39 so this sample was omitted from analyses. sperm   Total RNA extraction New England BioLabs kit NEBNext®Multiplex Small RNA Library Prep Set for Illumina® Set 1 and 2 NEB #E7300 and NEB #E7580 small RNA library prep for Illumina   cell line:sperm|genotype:WT|treatment:High2 GSM7916486 GSM7916486: Lb 1; Danio rerio; ncRNA Seq GSM7916486 r1 GSM7916486 1 Total RNA extraction New England BioLabs kit NEBNext®Multiplex Small RNA Library Prep Set for Illumina® Set 1 and 2 NEB #E7300 and NEB #E7580 small RNA library prep for Illumina   ncRNA-Seq TRANSCRIPTOMIC size fractionation SINGLE ILLUMINA Illumina HiSeq 2500   SRP473892     12235X11_160303_D00294_0224_AC95NJANXX_5.cutadapt.se.19_100.fastq.gz fastq 498695572.0 21269833.0 GSM7916486 r1 0:23.45 A:122716879;C:84997892;G:144626268;T:146292630;N:61903 23       122716879 84997892 144626268 146292630 61903 SRX22630087 SRS19628569 SRA1756943 University of East Anglia University of East Anglia 1 0.84251   0.1958   0.8591   0.61008   24   B   usable mapping rate illumina hiseq_era unknown size_fractionation nebnext bulk unknown unknown   United Kingdom 2023-11-23 Undetermined Embryo Cell Line Cell Line
29007 29007 SRR26936252 SRX22630086 SRS19628568 SRP473892 PRJNA1044439 Social stress in fathers affects sperm small RNA and offspring transcriptome profiles GSE248535 Transcriptome Analysis Environmental changes may affect paternal condition and following generations but the underlying mechanisms are poorly understood. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and development in their offspring. Here we investigated the role of sperm mediated small RNAs in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes and transposable elements TEs at this key developmental stage. We sequenced micro mi and Piwi interacting piRNAs in sperm and the full transcriptome in the embryos and ran differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from high to low competition environments. We identified 612 differentially expressed genes in the embryos. These results confirm that the social environment does not only affect males but also the molecular ecology of their sperm and the gene expression in their offspring suggesting a putative role of sRNAs. Overall design: This data deposit includes the small RNA seq data for this research. Male zebrafish were subject to high and low social stress environments with sperm samples collected RNA extracted and sent for small RNA sequencing.   pubmed:40121340   Hb 10 GSM7916485   tissue:sperm|cell line:sperm|genotype:WT|treatment:Low1|geo loc name:missing|collection date:missing Hb 10 Reads were trimmed aligned with PatMan and then count matrices assembled before DESeq2 analyses Assembly: GRCz10 Supplementary files format and content: piRNA raw counts.csv raw counts of a subset of 24 out of the 40 samples that were analysed. These 24 were chosen due to noise observed in PCA clustering. Supplementary files format and content: mirna raw counts.csv raw counts for 39/40 samples as one sample failed to progress through our pipeline to the same high quality as the other 39 so this sample was omitted from analyses. sperm   Total RNA extraction New England BioLabs kit NEBNext®Multiplex Small RNA Library Prep Set for Illumina® Set 1 and 2 NEB #E7300 and NEB #E7580 small RNA library prep for Illumina   cell line:sperm|genotype:WT|treatment:Low1 GSM7916485 GSM7916485: Hb 10; Danio rerio; ncRNA Seq GSM7916485 r1 GSM7916485 1 Total RNA extraction New England BioLabs kit NEBNext®Multiplex Small RNA Library Prep Set for Illumina® Set 1 and 2 NEB #E7300 and NEB #E7580 small RNA library prep for Illumina   ncRNA-Seq TRANSCRIPTOMIC size fractionation SINGLE ILLUMINA Illumina HiSeq 2500   SRP473892     12235X10_160303_D00294_0224_AC95NJANXX_5.cutadapt.se.19_100.fastq.gz fastq 686865211.0 29320389.0 GSM7916485 r1 0:23.43 A:156131568;C:132162427;G:201526230;T:196961742;N:83244 23       156131568 132162427 201526230 196961742 83244 SRX22630086 SRS19628568 SRA1756943 University of East Anglia University of East Anglia 1 0.85707   0.27856   0.85648   0.59717   28   B   usable mapping rate illumina hiseq_era unknown size_fractionation nebnext bulk unknown unknown   United Kingdom 2023-11-23 Undetermined Embryo Cell Line Cell Line
29008 29008 SRR26936253 SRX22630085 SRS19628567 SRP473892 PRJNA1044439 Social stress in fathers affects sperm small RNA and offspring transcriptome profiles GSE248535 Transcriptome Analysis Environmental changes may affect paternal condition and following generations but the underlying mechanisms are poorly understood. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and development in their offspring. Here we investigated the role of sperm mediated small RNAs in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes and transposable elements TEs at this key developmental stage. We sequenced micro mi and Piwi interacting piRNAs in sperm and the full transcriptome in the embryos and ran differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from high to low competition environments. We identified 612 differentially expressed genes in the embryos. These results confirm that the social environment does not only affect males but also the molecular ecology of their sperm and the gene expression in their offspring suggesting a putative role of sRNAs. Overall design: This data deposit includes the small RNA seq data for this research. Male zebrafish were subject to high and low social stress environments with sperm samples collected RNA extracted and sent for small RNA sequencing.   pubmed:40121340   La 9 GSM7916484   tissue:sperm|cell line:sperm|genotype:WT|treatment:High1|geo loc name:missing|collection date:missing La 9 Reads were trimmed aligned with PatMan and then count matrices assembled before DESeq2 analyses Assembly: GRCz10 Supplementary files format and content: piRNA raw counts.csv raw counts of a subset of 24 out of the 40 samples that were analysed. These 24 were chosen due to noise observed in PCA clustering. Supplementary files format and content: mirna raw counts.csv raw counts for 39/40 samples as one sample failed to progress through our pipeline to the same high quality as the other 39 so this sample was omitted from analyses. sperm   Total RNA extraction New England BioLabs kit NEBNext®Multiplex Small RNA Library Prep Set for Illumina® Set 1 and 2 NEB #E7300 and NEB #E7580 small RNA library prep for Illumina   cell line:sperm|genotype:WT|treatment:High1 GSM7916484 GSM7916484: La 9; Danio rerio; ncRNA Seq GSM7916484 r1 GSM7916484 1 Total RNA extraction New England BioLabs kit NEBNext®Multiplex Small RNA Library Prep Set for Illumina® Set 1 and 2 NEB #E7300 and NEB #E7580 small RNA library prep for Illumina   ncRNA-Seq TRANSCRIPTOMIC size fractionation SINGLE ILLUMINA Illumina HiSeq 2500   SRP473892     12235X9_160303_D00294_0224_AC95NJANXX_5.cutadapt.se.19_100.fastq.gz fastq 561289514.0 23860643.0 GSM7916484 r1 0:23.52 A:140848879;C:98838555;G:161520871;T:160013845;N:67364 23       140848879 98838555 161520871 160013845 67364 SRX22630085 SRS19628567 SRA1756943 University of East Anglia University of East Anglia 1 0.85507   0.17768   0.86969   0.64792   22   B   usable mapping rate illumina hiseq_era unknown size_fractionation nebnext bulk unknown unknown   United Kingdom 2023-11-23 Undetermined Embryo Cell Line Cell Line
29009 29009 SRR26936254 SRX22630084 SRS19628566 SRP473892 PRJNA1044439 Social stress in fathers affects sperm small RNA and offspring transcriptome profiles GSE248535 Transcriptome Analysis Environmental changes may affect paternal condition and following generations but the underlying mechanisms are poorly understood. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and development in their offspring. Here we investigated the role of sperm mediated small RNAs in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes and transposable elements TEs at this key developmental stage. We sequenced micro mi and Piwi interacting piRNAs in sperm and the full transcriptome in the embryos and ran differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from high to low competition environments. We identified 612 differentially expressed genes in the embryos. These results confirm that the social environment does not only affect males but also the molecular ecology of their sperm and the gene expression in their offspring suggesting a putative role of sRNAs. Overall design: This data deposit includes the small RNA seq data for this research. Male zebrafish were subject to high and low social stress environments with sperm samples collected RNA extracted and sent for small RNA sequencing.   pubmed:40121340   La 8 GSM7916483   tissue:sperm|cell line:sperm|genotype:WT|treatment:Low2|geo loc name:missing|collection date:missing La 8 Reads were trimmed aligned with PatMan and then count matrices assembled before DESeq2 analyses Assembly: GRCz10 Supplementary files format and content: piRNA raw counts.csv raw counts of a subset of 24 out of the 40 samples that were analysed. These 24 were chosen due to noise observed in PCA clustering. Supplementary files format and content: mirna raw counts.csv raw counts for 39/40 samples as one sample failed to progress through our pipeline to the same high quality as the other 39 so this sample was omitted from analyses. sperm   Total RNA extraction New England BioLabs kit NEBNext®Multiplex Small RNA Library Prep Set for Illumina® Set 1 and 2 NEB #E7300 and NEB #E7580 small RNA library prep for Illumina   cell line:sperm|genotype:WT|treatment:Low2 GSM7916483 GSM7916483: La 8; Danio rerio; ncRNA Seq GSM7916483 r1 GSM7916483 1 Total RNA extraction New England BioLabs kit NEBNext®Multiplex Small RNA Library Prep Set for Illumina® Set 1 and 2 NEB #E7300 and NEB #E7580 small RNA library prep for Illumina   ncRNA-Seq TRANSCRIPTOMIC size fractionation SINGLE ILLUMINA Illumina HiSeq 2500   SRP473892     12235X8_160303_D00294_0224_AC95NJANXX_4.cutadapt.se.19_100.fastq.gz fastq 458491681.0 16224249.0 GSM7916483 r1 0:28.26 A:99631677;C:90884289;G:139467457;T:128470855;N:37403 28       99631677 90884289 139467457 128470855 37403 SRX22630084 SRS19628566 SRA1756943 University of East Anglia University of East Anglia 1 0.81704   0.21909   0.84762   0.6293   50   B   usable mapping rate illumina hiseq_era unknown size_fractionation nebnext bulk unknown unknown   United Kingdom 2023-11-23 Undetermined Embryo Cell Line Cell Line
29010 29010 SRR26936255 SRX22630083 SRS19628565 SRP473892 PRJNA1044439 Social stress in fathers affects sperm small RNA and offspring transcriptome profiles GSE248535 Transcriptome Analysis Environmental changes may affect paternal condition and following generations but the underlying mechanisms are poorly understood. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and development in their offspring. Here we investigated the role of sperm mediated small RNAs in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes and transposable elements TEs at this key developmental stage. We sequenced micro mi and Piwi interacting piRNAs in sperm and the full transcriptome in the embryos and ran differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from high to low competition environments. We identified 612 differentially expressed genes in the embryos. These results confirm that the social environment does not only affect males but also the molecular ecology of their sperm and the gene expression in their offspring suggesting a putative role of sRNAs. Overall design: This data deposit includes the small RNA seq data for this research. Male zebrafish were subject to high and low social stress environments with sperm samples collected RNA extracted and sent for small RNA sequencing.   pubmed:40121340   Ha 9 GSM7916482   tissue:sperm|cell line:sperm|genotype:WT|treatment:Low1|geo loc name:missing|collection date:missing Ha 9 Reads were trimmed aligned with PatMan and then count matrices assembled before DESeq2 analyses Assembly: GRCz10 Supplementary files format and content: piRNA raw counts.csv raw counts of a subset of 24 out of the 40 samples that were analysed. These 24 were chosen due to noise observed in PCA clustering. Supplementary files format and content: mirna raw counts.csv raw counts for 39/40 samples as one sample failed to progress through our pipeline to the same high quality as the other 39 so this sample was omitted from analyses. sperm   Total RNA extraction New England BioLabs kit NEBNext®Multiplex Small RNA Library Prep Set for Illumina® Set 1 and 2 NEB #E7300 and NEB #E7580 small RNA library prep for Illumina   cell line:sperm|genotype:WT|treatment:Low1 GSM7916482 GSM7916482: Ha 9; Danio rerio; ncRNA Seq GSM7916482 r1 GSM7916482 1 Total RNA extraction New England BioLabs kit NEBNext®Multiplex Small RNA Library Prep Set for Illumina® Set 1 and 2 NEB #E7300 and NEB #E7580 small RNA library prep for Illumina   ncRNA-Seq TRANSCRIPTOMIC size fractionation SINGLE ILLUMINA Illumina HiSeq 2500   SRP473892     12235X7_160303_D00294_0224_AC95NJANXX_4.cutadapt.se.19_100.fastq.gz fastq 574511047.0 20295283.0 GSM7916482 r1 0:28.31 A:134481373;C:122719422;G:169313406;T:147944946;N:51900 28       134481373 122719422 169313406 147944946 51900 SRX22630083 SRS19628565 SRA1756943 University of East Anglia University of East Anglia 1 0.81932   0.19984   0.85987   0.58268   23   B   usable mapping rate illumina hiseq_era unknown size_fractionation nebnext bulk unknown unknown   United Kingdom 2023-11-23 Undetermined Embryo Cell Line Cell Line
29011 29011 SRR26936256 SRX22630082 SRS19628564 SRP473892 PRJNA1044439 Social stress in fathers affects sperm small RNA and offspring transcriptome profiles GSE248535 Transcriptome Analysis Environmental changes may affect paternal condition and following generations but the underlying mechanisms are poorly understood. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and development in their offspring. Here we investigated the role of sperm mediated small RNAs in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes and transposable elements TEs at this key developmental stage. We sequenced micro mi and Piwi interacting piRNAs in sperm and the full transcriptome in the embryos and ran differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from high to low competition environments. We identified 612 differentially expressed genes in the embryos. These results confirm that the social environment does not only affect males but also the molecular ecology of their sperm and the gene expression in their offspring suggesting a putative role of sRNAs. Overall design: This data deposit includes the small RNA seq data for this research. Male zebrafish were subject to high and low social stress environments with sperm samples collected RNA extracted and sent for small RNA sequencing.   pubmed:40121340   Lb 8 GSM7916481   tissue:sperm|cell line:sperm|genotype:WT|treatment:Low2|geo loc name:missing|collection date:missing Lb 8 Reads were trimmed aligned with PatMan and then count matrices assembled before DESeq2 analyses Assembly: GRCz10 Supplementary files format and content: piRNA raw counts.csv raw counts of a subset of 24 out of the 40 samples that were analysed. These 24 were chosen due to noise observed in PCA clustering. Supplementary files format and content: mirna raw counts.csv raw counts for 39/40 samples as one sample failed to progress through our pipeline to the same high quality as the other 39 so this sample was omitted from analyses. sperm   Total RNA extraction New England BioLabs kit NEBNext®Multiplex Small RNA Library Prep Set for Illumina® Set 1 and 2 NEB #E7300 and NEB #E7580 small RNA library prep for Illumina   cell line:sperm|genotype:WT|treatment:Low2 GSM7916481 GSM7916481: Lb 8; Danio rerio; ncRNA Seq GSM7916481 r1 GSM7916481 1 Total RNA extraction New England BioLabs kit NEBNext®Multiplex Small RNA Library Prep Set for Illumina® Set 1 and 2 NEB #E7300 and NEB #E7580 small RNA library prep for Illumina   ncRNA-Seq TRANSCRIPTOMIC size fractionation SINGLE ILLUMINA Illumina HiSeq 2500   SRP473892     12235X6_160303_D00294_0224_AC95NJANXX_4.cutadapt.se.19_100.fastq.gz fastq 1086899780.0 37486748.0 GSM7916481 r1 0:28.99 A:219996636;C:232899819;G:335644980;T:298269921;N:88424 28       219996636 232899819 335644980 298269921 88424 SRX22630082 SRS19628564 SRA1756943 University of East Anglia University of East Anglia 1 0.86943   0.2105   0.84461   0.67703   44   B   usable mapping rate illumina hiseq_era unknown size_fractionation nebnext bulk unknown unknown   United Kingdom 2023-11-23 Undetermined Embryo Cell Line Cell Line
29012 29012 SRR26936257 SRX22630081 SRS19628563 SRP473892 PRJNA1044439 Social stress in fathers affects sperm small RNA and offspring transcriptome profiles GSE248535 Transcriptome Analysis Environmental changes may affect paternal condition and following generations but the underlying mechanisms are poorly understood. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and development in their offspring. Here we investigated the role of sperm mediated small RNAs in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes and transposable elements TEs at this key developmental stage. We sequenced micro mi and Piwi interacting piRNAs in sperm and the full transcriptome in the embryos and ran differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from high to low competition environments. We identified 612 differentially expressed genes in the embryos. These results confirm that the social environment does not only affect males but also the molecular ecology of their sperm and the gene expression in their offspring suggesting a putative role of sRNAs. Overall design: This data deposit includes the small RNA seq data for this research. Male zebrafish were subject to high and low social stress environments with sperm samples collected RNA extracted and sent for small RNA sequencing.   pubmed:40121340   Hb 9 GSM7916480   tissue:sperm|cell line:sperm|genotype:WT|treatment:High2|geo loc name:missing|collection date:missing Hb 9 Reads were trimmed aligned with PatMan and then count matrices assembled before DESeq2 analyses Assembly: GRCz10 Supplementary files format and content: piRNA raw counts.csv raw counts of a subset of 24 out of the 40 samples that were analysed. These 24 were chosen due to noise observed in PCA clustering. Supplementary files format and content: mirna raw counts.csv raw counts for 39/40 samples as one sample failed to progress through our pipeline to the same high quality as the other 39 so this sample was omitted from analyses. sperm   Total RNA extraction New England BioLabs kit NEBNext®Multiplex Small RNA Library Prep Set for Illumina® Set 1 and 2 NEB #E7300 and NEB #E7580 small RNA library prep for Illumina   cell line:sperm|genotype:WT|treatment:High2 GSM7916480 GSM7916480: Hb 9; Danio rerio; ncRNA Seq GSM7916480 r1 GSM7916480 1 Total RNA extraction New England BioLabs kit NEBNext®Multiplex Small RNA Library Prep Set for Illumina® Set 1 and 2 NEB #E7300 and NEB #E7580 small RNA library prep for Illumina   ncRNA-Seq TRANSCRIPTOMIC size fractionation SINGLE ILLUMINA Illumina HiSeq 2500   SRP473892     12235X5_160303_D00294_0224_AC95NJANXX_4.cutadapt.se.19_100.fastq.gz fastq 1144093618.0 39048750.0 GSM7916480 r1 0:29.30 A:223769668;C:238953593;G:358148504;T:323130325;N:91528 29       223769668 238953593 358148504 323130325 91528 SRX22630081 SRS19628563 SRA1756943 University of East Anglia University of East Anglia 1 0.87445   0.1906   0.87237   0.70919   50   B   usable mapping rate illumina hiseq_era unknown size_fractionation nebnext bulk unknown unknown   United Kingdom 2023-11-23 Undetermined Embryo Cell Line Cell Line
29013 29013 SRR26936258 SRX22630080 SRS19628562 SRP473892 PRJNA1044439 Social stress in fathers affects sperm small RNA and offspring transcriptome profiles GSE248535 Transcriptome Analysis Environmental changes may affect paternal condition and following generations but the underlying mechanisms are poorly understood. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and development in their offspring. Here we investigated the role of sperm mediated small RNAs in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes and transposable elements TEs at this key developmental stage. We sequenced micro mi and Piwi interacting piRNAs in sperm and the full transcriptome in the embryos and ran differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from high to low competition environments. We identified 612 differentially expressed genes in the embryos. These results confirm that the social environment does not only affect males but also the molecular ecology of their sperm and the gene expression in their offspring suggesting a putative role of sRNAs. Overall design: This data deposit includes the small RNA seq data for this research. Male zebrafish were subject to high and low social stress environments with sperm samples collected RNA extracted and sent for small RNA sequencing.   pubmed:40121340   Hb 7 GSM7916515   tissue:sperm|cell line:sperm|genotype:WT|treatment:High2|geo loc name:missing|collection date:missing Hb 7 Reads were trimmed aligned with PatMan and then count matrices assembled before DESeq2 analyses Assembly: GRCz10 Supplementary files format and content: piRNA raw counts.csv raw counts of a subset of 24 out of the 40 samples that were analysed. These 24 were chosen due to noise observed in PCA clustering. Supplementary files format and content: mirna raw counts.csv raw counts for 39/40 samples as one sample failed to progress through our pipeline to the same high quality as the other 39 so this sample was omitted from analyses. sperm   Total RNA extraction New England BioLabs kit NEBNext®Multiplex Small RNA Library Prep Set for Illumina® Set 1 and 2 NEB #E7300 and NEB #E7580 small RNA library prep for Illumina   cell line:sperm|genotype:WT|treatment:High2 GSM7916515 GSM7916515: Hb 7; Danio rerio; ncRNA Seq GSM7916515 r1 GSM7916515 1 Total RNA extraction New England BioLabs kit NEBNext®Multiplex Small RNA Library Prep Set for Illumina® Set 1 and 2 NEB #E7300 and NEB #E7580 small RNA library prep for Illumina   ncRNA-Seq TRANSCRIPTOMIC size fractionation SINGLE ILLUMINA Illumina HiSeq 2500   SRP473892     12235X40_160303_D00294_0224_AC95NJANXX_8.cutadapt.se.19_100.fastq.gz fastq 263782568.0 9802454.0 GSM7916515 r1 0:26.91 A:59378855;C:53589034;G:83219251;T:67574216;N:21212 26       59378855 53589034 83219251 67574216 21212 SRX22630080 SRS19628562 SRA1756943 University of East Anglia University of East Anglia 1 0.80146   0.31524   0.81008   0.47305   30   B   usable mapping rate illumina hiseq_era unknown size_fractionation nebnext bulk unknown unknown   United Kingdom 2023-11-23 Undetermined Embryo Cell Line Cell Line
29014 29014 SRR26936260 SRX22630079 SRS19628561 SRP473892 PRJNA1044439 Social stress in fathers affects sperm small RNA and offspring transcriptome profiles GSE248535 Transcriptome Analysis Environmental changes may affect paternal condition and following generations but the underlying mechanisms are poorly understood. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and development in their offspring. Here we investigated the role of sperm mediated small RNAs in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes and transposable elements TEs at this key developmental stage. We sequenced micro mi and Piwi interacting piRNAs in sperm and the full transcriptome in the embryos and ran differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from high to low competition environments. We identified 612 differentially expressed genes in the embryos. These results confirm that the social environment does not only affect males but also the molecular ecology of their sperm and the gene expression in their offspring suggesting a putative role of sRNAs. Overall design: This data deposit includes the small RNA seq data for this research. Male zebrafish were subject to high and low social stress environments with sperm samples collected RNA extracted and sent for small RNA sequencing.   pubmed:40121340   Ha 8 GSM7916514   tissue:sperm|cell line:sperm|genotype:WT|treatment:High1|geo loc name:missing|collection date:missing Ha 8 Reads were trimmed aligned with PatMan and then count matrices assembled before DESeq2 analyses Assembly: GRCz10 Supplementary files format and content: piRNA raw counts.csv raw counts of a subset of 24 out of the 40 samples that were analysed. These 24 were chosen due to noise observed in PCA clustering. Supplementary files format and content: mirna raw counts.csv raw counts for 39/40 samples as one sample failed to progress through our pipeline to the same high quality as the other 39 so this sample was omitted from analyses. sperm   Total RNA extraction New England BioLabs kit NEBNext®Multiplex Small RNA Library Prep Set for Illumina® Set 1 and 2 NEB #E7300 and NEB #E7580 small RNA library prep for Illumina   cell line:sperm|genotype:WT|treatment:High1 GSM7916514 GSM7916514: Ha 8; Danio rerio; ncRNA Seq GSM7916514 r1 GSM7916514 1 Total RNA extraction New England BioLabs kit NEBNext®Multiplex Small RNA Library Prep Set for Illumina® Set 1 and 2 NEB #E7300 and NEB #E7580 small RNA library prep for Illumina   ncRNA-Seq TRANSCRIPTOMIC size fractionation SINGLE ILLUMINA Illumina HiSeq 2500   SRP473892     12235X39_160303_D00294_0224_AC95NJANXX_8.cutadapt.se.19_100.fastq.gz fastq 252119227.0 9309447.0 GSM7916514 r1 0:27.08 A:53293803;C:51577689;G:79148986;T:68078043;N:20706 27       53293803 51577689 79148986 68078043 20706 SRX22630079 SRS19628561 SRA1756943 University of East Anglia University of East Anglia 1 0.75593   0.29042   0.83412   0.59657   21   B   usable mapping rate illumina hiseq_era unknown size_fractionation nebnext bulk unknown unknown   United Kingdom 2023-11-23 Undetermined Embryo Cell Line Cell Line
29015 29015 SRR26936261 SRX22630078 SRS19628560 SRP473892 PRJNA1044439 Social stress in fathers affects sperm small RNA and offspring transcriptome profiles GSE248535 Transcriptome Analysis Environmental changes may affect paternal condition and following generations but the underlying mechanisms are poorly understood. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and development in their offspring. Here we investigated the role of sperm mediated small RNAs in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes and transposable elements TEs at this key developmental stage. We sequenced micro mi and Piwi interacting piRNAs in sperm and the full transcriptome in the embryos and ran differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from high to low competition environments. We identified 612 differentially expressed genes in the embryos. These results confirm that the social environment does not only affect males but also the molecular ecology of their sperm and the gene expression in their offspring suggesting a putative role of sRNAs. Overall design: This data deposit includes the small RNA seq data for this research. Male zebrafish were subject to high and low social stress environments with sperm samples collected RNA extracted and sent for small RNA sequencing.   pubmed:40121340   Hb 6 GSM7916513   tissue:sperm|cell line:sperm|genotype:WT|treatment:High2|geo loc name:missing|collection date:missing Hb 6 Reads were trimmed aligned with PatMan and then count matrices assembled before DESeq2 analyses Assembly: GRCz10 Supplementary files format and content: piRNA raw counts.csv raw counts of a subset of 24 out of the 40 samples that were analysed. These 24 were chosen due to noise observed in PCA clustering. Supplementary files format and content: mirna raw counts.csv raw counts for 39/40 samples as one sample failed to progress through our pipeline to the same high quality as the other 39 so this sample was omitted from analyses. sperm   Total RNA extraction New England BioLabs kit NEBNext®Multiplex Small RNA Library Prep Set for Illumina® Set 1 and 2 NEB #E7300 and NEB #E7580 small RNA library prep for Illumina   cell line:sperm|genotype:WT|treatment:High2 GSM7916513 GSM7916513: Hb 6; Danio rerio; ncRNA Seq GSM7916513 r1 GSM7916513 1 Total RNA extraction New England BioLabs kit NEBNext®Multiplex Small RNA Library Prep Set for Illumina® Set 1 and 2 NEB #E7300 and NEB #E7580 small RNA library prep for Illumina   ncRNA-Seq TRANSCRIPTOMIC size fractionation SINGLE ILLUMINA Illumina HiSeq 2500   SRP473892     12235X38_160303_D00294_0224_AC95NJANXX_8.cutadapt.se.19_100.fastq.gz fastq 516408240.0 19719085.0 GSM7916513 r1 0:26.19 A:112791146;C:102652986;G:160354602;T:140566375;N:43131 26       112791146 102652986 160354602 140566375 43131 SRX22630078 SRS19628560 SRA1756943 University of East Anglia University of East Anglia 1 0.80401   0.31982   0.83765   0.57755   22   B   usable mapping rate illumina hiseq_era unknown size_fractionation nebnext bulk unknown unknown   United Kingdom 2023-11-23 Undetermined Embryo Cell Line Cell Line
29016 29016 SRR26936262 SRX22630077 SRS19628559 SRP473892 PRJNA1044439 Social stress in fathers affects sperm small RNA and offspring transcriptome profiles GSE248535 Transcriptome Analysis Environmental changes may affect paternal condition and following generations but the underlying mechanisms are poorly understood. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and development in their offspring. Here we investigated the role of sperm mediated small RNAs in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes and transposable elements TEs at this key developmental stage. We sequenced micro mi and Piwi interacting piRNAs in sperm and the full transcriptome in the embryos and ran differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from high to low competition environments. We identified 612 differentially expressed genes in the embryos. These results confirm that the social environment does not only affect males but also the molecular ecology of their sperm and the gene expression in their offspring suggesting a putative role of sRNAs. Overall design: This data deposit includes the small RNA seq data for this research. Male zebrafish were subject to high and low social stress environments with sperm samples collected RNA extracted and sent for small RNA sequencing.   pubmed:40121340   Ha 7 GSM7916512   tissue:sperm|cell line:sperm|genotype:WT|treatment:High1|geo loc name:missing|collection date:missing Ha 7 Reads were trimmed aligned with PatMan and then count matrices assembled before DESeq2 analyses Assembly: GRCz10 Supplementary files format and content: piRNA raw counts.csv raw counts of a subset of 24 out of the 40 samples that were analysed. These 24 were chosen due to noise observed in PCA clustering. Supplementary files format and content: mirna raw counts.csv raw counts for 39/40 samples as one sample failed to progress through our pipeline to the same high quality as the other 39 so this sample was omitted from analyses. sperm   Total RNA extraction New England BioLabs kit NEBNext®Multiplex Small RNA Library Prep Set for Illumina® Set 1 and 2 NEB #E7300 and NEB #E7580 small RNA library prep for Illumina   cell line:sperm|genotype:WT|treatment:High1 GSM7916512 GSM7916512: Ha 7; Danio rerio; ncRNA Seq GSM7916512 r1 GSM7916512 1 Total RNA extraction New England BioLabs kit NEBNext®Multiplex Small RNA Library Prep Set for Illumina® Set 1 and 2 NEB #E7300 and NEB #E7580 small RNA library prep for Illumina   ncRNA-Seq TRANSCRIPTOMIC size fractionation SINGLE ILLUMINA Illumina HiSeq 2500   SRP473892     12235X37_160303_D00294_0224_AC95NJANXX_8.cutadapt.se.19_100.fastq.gz fastq 554171558.0 20779285.0 GSM7916512 r1 0:26.67 A:125256371;C:111354628;G:172197000;T:145317427;N:46132 26       125256371 111354628 172197000 145317427 46132 SRX22630077 SRS19628559 SRA1756943 University of East Anglia University of East Anglia 1 0.81035   0.28326   0.82933   0.62525   35   B   usable mapping rate illumina hiseq_era unknown size_fractionation nebnext bulk unknown unknown   United Kingdom 2023-11-23 Undetermined Embryo Cell Line Cell Line
29017 29017 SRR26936263 SRX22630076 SRS19628558 SRP473892 PRJNA1044439 Social stress in fathers affects sperm small RNA and offspring transcriptome profiles GSE248535 Transcriptome Analysis Environmental changes may affect paternal condition and following generations but the underlying mechanisms are poorly understood. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and development in their offspring. Here we investigated the role of sperm mediated small RNAs in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes and transposable elements TEs at this key developmental stage. We sequenced micro mi and Piwi interacting piRNAs in sperm and the full transcriptome in the embryos and ran differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from high to low competition environments. We identified 612 differentially expressed genes in the embryos. These results confirm that the social environment does not only affect males but also the molecular ecology of their sperm and the gene expression in their offspring suggesting a putative role of sRNAs. Overall design: This data deposit includes the small RNA seq data for this research. Male zebrafish were subject to high and low social stress environments with sperm samples collected RNA extracted and sent for small RNA sequencing.   pubmed:40121340   Lb 10 GSM7916511   tissue:sperm|cell line:sperm|genotype:WT|treatment:Low2|geo loc name:missing|collection date:missing Lb 10 Reads were trimmed aligned with PatMan and then count matrices assembled before DESeq2 analyses Assembly: GRCz10 Supplementary files format and content: piRNA raw counts.csv raw counts of a subset of 24 out of the 40 samples that were analysed. These 24 were chosen due to noise observed in PCA clustering. Supplementary files format and content: mirna raw counts.csv raw counts for 39/40 samples as one sample failed to progress through our pipeline to the same high quality as the other 39 so this sample was omitted from analyses. sperm   Total RNA extraction New England BioLabs kit NEBNext®Multiplex Small RNA Library Prep Set for Illumina® Set 1 and 2 NEB #E7300 and NEB #E7580 small RNA library prep for Illumina   cell line:sperm|genotype:WT|treatment:Low2 GSM7916511 GSM7916511: Lb 10; Danio rerio; ncRNA Seq GSM7916511 r1 GSM7916511 1 Total RNA extraction New England BioLabs kit NEBNext®Multiplex Small RNA Library Prep Set for Illumina® Set 1 and 2 NEB #E7300 and NEB #E7580 small RNA library prep for Illumina   ncRNA-Seq TRANSCRIPTOMIC size fractionation SINGLE ILLUMINA Illumina HiSeq 2500   SRP473892     12235X36_160303_D00294_0224_AC95NJANXX_8.cutadapt.se.19_100.fastq.gz fastq 983846643.0 32513493.0 GSM7916511 r1 0:30.26 A:181840038;C:211101778;G:320659770;T:270174775;N:70282 30       181840038 211101778 320659770 270174775 70282 SRX22630076 SRS19628558 SRA1756943 University of East Anglia University of East Anglia 1 0.88133   0.15829   0.86324   0.67761   37   B   usable mapping rate illumina hiseq_era unknown size_fractionation nebnext bulk unknown unknown   United Kingdom 2023-11-23 Undetermined Embryo Cell Line Cell Line
29018 29018 SRR26936264 SRX22630075 SRS19628557 SRP473892 PRJNA1044439 Social stress in fathers affects sperm small RNA and offspring transcriptome profiles GSE248535 Transcriptome Analysis Environmental changes may affect paternal condition and following generations but the underlying mechanisms are poorly understood. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and development in their offspring. Here we investigated the role of sperm mediated small RNAs in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes and transposable elements TEs at this key developmental stage. We sequenced micro mi and Piwi interacting piRNAs in sperm and the full transcriptome in the embryos and ran differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from high to low competition environments. We identified 612 differentially expressed genes in the embryos. These results confirm that the social environment does not only affect males but also the molecular ecology of their sperm and the gene expression in their offspring suggesting a putative role of sRNAs. Overall design: This data deposit includes the small RNA seq data for this research. Male zebrafish were subject to high and low social stress environments with sperm samples collected RNA extracted and sent for small RNA sequencing.   pubmed:40121340   Lb 9 GSM7916510   tissue:sperm|cell line:sperm|genotype:WT|treatment:Low2|geo loc name:missing|collection date:missing Lb 9 Reads were trimmed aligned with PatMan and then count matrices assembled before DESeq2 analyses Assembly: GRCz10 Supplementary files format and content: piRNA raw counts.csv raw counts of a subset of 24 out of the 40 samples that were analysed. These 24 were chosen due to noise observed in PCA clustering. Supplementary files format and content: mirna raw counts.csv raw counts for 39/40 samples as one sample failed to progress through our pipeline to the same high quality as the other 39 so this sample was omitted from analyses. sperm   Total RNA extraction New England BioLabs kit NEBNext®Multiplex Small RNA Library Prep Set for Illumina® Set 1 and 2 NEB #E7300 and NEB #E7580 small RNA library prep for Illumina   cell line:sperm|genotype:WT|treatment:Low2 GSM7916510 GSM7916510: Lb 9; Danio rerio; ncRNA Seq GSM7916510 r1 GSM7916510 1 Total RNA extraction New England BioLabs kit NEBNext®Multiplex Small RNA Library Prep Set for Illumina® Set 1 and 2 NEB #E7300 and NEB #E7580 small RNA library prep for Illumina   ncRNA-Seq TRANSCRIPTOMIC size fractionation SINGLE ILLUMINA Illumina HiSeq 2500   SRP473892     12235X35_160303_D00294_0224_AC95NJANXX_8.cutadapt.se.19_100.fastq.gz fastq 1077131964.0 37869598.0 GSM7916510 r1 0:28.44 A:210508796;C:220304812;G:327237157;T:319001223;N:79976 28       210508796 220304812 327237157 319001223 79976 SRX22630075 SRS19628557 SRA1756943 University of East Anglia University of East Anglia 1 0.84871   0.22867   0.83968   0.66027   35   B   usable mapping rate illumina hiseq_era unknown size_fractionation nebnext bulk unknown unknown   United Kingdom 2023-11-23 Undetermined Embryo Cell Line Cell Line
29019 29019 SRR26936265 SRX22630074 SRS19628556 SRP473892 PRJNA1044439 Social stress in fathers affects sperm small RNA and offspring transcriptome profiles GSE248535 Transcriptome Analysis Environmental changes may affect paternal condition and following generations but the underlying mechanisms are poorly understood. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and development in their offspring. Here we investigated the role of sperm mediated small RNAs in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes and transposable elements TEs at this key developmental stage. We sequenced micro mi and Piwi interacting piRNAs in sperm and the full transcriptome in the embryos and ran differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from high to low competition environments. We identified 612 differentially expressed genes in the embryos. These results confirm that the social environment does not only affect males but also the molecular ecology of their sperm and the gene expression in their offspring suggesting a putative role of sRNAs. Overall design: This data deposit includes the small RNA seq data for this research. Male zebrafish were subject to high and low social stress environments with sperm samples collected RNA extracted and sent for small RNA sequencing.   pubmed:40121340   Lb 7 GSM7916509   tissue:sperm|cell line:sperm|genotype:WT|treatment:Low1|geo loc name:missing|collection date:missing Lb 7 Reads were trimmed aligned with PatMan and then count matrices assembled before DESeq2 analyses Assembly: GRCz10 Supplementary files format and content: piRNA raw counts.csv raw counts of a subset of 24 out of the 40 samples that were analysed. These 24 were chosen due to noise observed in PCA clustering. Supplementary files format and content: mirna raw counts.csv raw counts for 39/40 samples as one sample failed to progress through our pipeline to the same high quality as the other 39 so this sample was omitted from analyses. sperm   Total RNA extraction New England BioLabs kit NEBNext®Multiplex Small RNA Library Prep Set for Illumina® Set 1 and 2 NEB #E7300 and NEB #E7580 small RNA library prep for Illumina   cell line:sperm|genotype:WT|treatment:Low1 GSM7916509 GSM7916509: Lb 7; Danio rerio; ncRNA Seq GSM7916509 r1 GSM7916509 1 Total RNA extraction New England BioLabs kit NEBNext®Multiplex Small RNA Library Prep Set for Illumina® Set 1 and 2 NEB #E7300 and NEB #E7580 small RNA library prep for Illumina   ncRNA-Seq TRANSCRIPTOMIC size fractionation SINGLE ILLUMINA Illumina HiSeq 2500   SRP473892     12235X34_160303_D00294_0224_AC95NJANXX_8.cutadapt.se.19_100.fastq.gz fastq 1203153474.0 42565421.0 GSM7916509 r1 0:28.27 A:269760359;C:263088083;G:348288367;T:321910116;N:106549 28       269760359 263088083 348288367 321910116 106549 SRX22630074 SRS19628556 SRA1756943 University of East Anglia University of East Anglia 1 0.82319   0.21661   0.82432   0.65954   33   B   usable mapping rate illumina hiseq_era unknown size_fractionation nebnext bulk unknown unknown   United Kingdom 2023-11-23 Undetermined Embryo Cell Line Cell Line
29020 29020 SRR26936266 SRX22630073 SRS19628555 SRP473892 PRJNA1044439 Social stress in fathers affects sperm small RNA and offspring transcriptome profiles GSE248535 Transcriptome Analysis Environmental changes may affect paternal condition and following generations but the underlying mechanisms are poorly understood. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and development in their offspring. Here we investigated the role of sperm mediated small RNAs in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes and transposable elements TEs at this key developmental stage. We sequenced micro mi and Piwi interacting piRNAs in sperm and the full transcriptome in the embryos and ran differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from high to low competition environments. We identified 612 differentially expressed genes in the embryos. These results confirm that the social environment does not only affect males but also the molecular ecology of their sperm and the gene expression in their offspring suggesting a putative role of sRNAs. Overall design: This data deposit includes the small RNA seq data for this research. Male zebrafish were subject to high and low social stress environments with sperm samples collected RNA extracted and sent for small RNA sequencing.   pubmed:40121340   La 7 GSM7916508   tissue:sperm|cell line:sperm|genotype:WT|treatment:Low1|geo loc name:missing|collection date:missing La 7 Reads were trimmed aligned with PatMan and then count matrices assembled before DESeq2 analyses Assembly: GRCz10 Supplementary files format and content: piRNA raw counts.csv raw counts of a subset of 24 out of the 40 samples that were analysed. These 24 were chosen due to noise observed in PCA clustering. Supplementary files format and content: mirna raw counts.csv raw counts for 39/40 samples as one sample failed to progress through our pipeline to the same high quality as the other 39 so this sample was omitted from analyses. sperm   Total RNA extraction New England BioLabs kit NEBNext®Multiplex Small RNA Library Prep Set for Illumina® Set 1 and 2 NEB #E7300 and NEB #E7580 small RNA library prep for Illumina   cell line:sperm|genotype:WT|treatment:Low1 GSM7916508 GSM7916508: La 7; Danio rerio; ncRNA Seq GSM7916508 r1 GSM7916508 1 Total RNA extraction New England BioLabs kit NEBNext®Multiplex Small RNA Library Prep Set for Illumina® Set 1 and 2 NEB #E7300 and NEB #E7580 small RNA library prep for Illumina   ncRNA-Seq TRANSCRIPTOMIC size fractionation SINGLE ILLUMINA Illumina HiSeq 2500   SRP473892     12235X33_160303_D00294_0224_AC95NJANXX_8.cutadapt.se.19_100.fastq.gz fastq 1137464666.0 42434385.0 GSM7916508 r1 0:26.81 A:240256314;C:218420241;G:336730784;T:341968066;N:89261 26       240256314 218420241 336730784 341968066 89261 SRX22630073 SRS19628555 SRA1756943 University of East Anglia University of East Anglia 1 0.84647   0.26357   0.85313   0.67665   22   B   usable mapping rate illumina hiseq_era unknown size_fractionation nebnext bulk unknown unknown   United Kingdom 2023-11-23 Undetermined Embryo Cell Line Cell Line
29021 29021 SRR26936259 SRX22630072 SRS19628554 SRP473892 PRJNA1044439 Social stress in fathers affects sperm small RNA and offspring transcriptome profiles GSE248535 Transcriptome Analysis Environmental changes may affect paternal condition and following generations but the underlying mechanisms are poorly understood. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and development in their offspring. Here we investigated the role of sperm mediated small RNAs in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes and transposable elements TEs at this key developmental stage. We sequenced micro mi and Piwi interacting piRNAs in sperm and the full transcriptome in the embryos and ran differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from high to low competition environments. We identified 612 differentially expressed genes in the embryos. These results confirm that the social environment does not only affect males but also the molecular ecology of their sperm and the gene expression in their offspring suggesting a putative role of sRNAs. Overall design: This data deposit includes the small RNA seq data for this research. Male zebrafish were subject to high and low social stress environments with sperm samples collected RNA extracted and sent for small RNA sequencing.   pubmed:40121340   Hb 8 GSM7916479   tissue:sperm|cell line:sperm|genotype:WT|treatment:High2|geo loc name:missing|collection date:missing Hb 8 Reads were trimmed aligned with PatMan and then count matrices assembled before DESeq2 analyses Assembly: GRCz10 Supplementary files format and content: piRNA raw counts.csv raw counts of a subset of 24 out of the 40 samples that were analysed. These 24 were chosen due to noise observed in PCA clustering. Supplementary files format and content: mirna raw counts.csv raw counts for 39/40 samples as one sample failed to progress through our pipeline to the same high quality as the other 39 so this sample was omitted from analyses. sperm   Total RNA extraction New England BioLabs kit NEBNext®Multiplex Small RNA Library Prep Set for Illumina® Set 1 and 2 NEB #E7300 and NEB #E7580 small RNA library prep for Illumina   cell line:sperm|genotype:WT|treatment:High2 GSM7916479 GSM7916479: Hb 8; Danio rerio; ncRNA Seq GSM7916479 r1 GSM7916479 1 Total RNA extraction New England BioLabs kit NEBNext®Multiplex Small RNA Library Prep Set for Illumina® Set 1 and 2 NEB #E7300 and NEB #E7580 small RNA library prep for Illumina   ncRNA-Seq TRANSCRIPTOMIC size fractionation SINGLE ILLUMINA Illumina HiSeq 2500   SRP473892     12235X4_160303_D00294_0224_AC95NJANXX_4.cutadapt.se.19_100.fastq.gz fastq 1014850001.0 36540733.0 GSM7916479 r1 0:27.77 A:205870762;C:199482442;G:294628769;T:314783528;N:84500 27       205870762 199482442 294628769 314783528 84500 SRX22630072 SRS19628554 SRA1756943 University of East Anglia University of East Anglia 1 0.84303   0.28884   0.81692   0.66059   26   B   usable mapping rate illumina hiseq_era unknown size_fractionation nebnext bulk unknown unknown   United Kingdom 2023-11-23 Undetermined Embryo Cell Line Cell Line
29022 29022 SRR26936268 SRX22630071 SRS19628553 SRP473892 PRJNA1044439 Social stress in fathers affects sperm small RNA and offspring transcriptome profiles GSE248535 Transcriptome Analysis Environmental changes may affect paternal condition and following generations but the underlying mechanisms are poorly understood. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and development in their offspring. Here we investigated the role of sperm mediated small RNAs in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes and transposable elements TEs at this key developmental stage. We sequenced micro mi and Piwi interacting piRNAs in sperm and the full transcriptome in the embryos and ran differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from high to low competition environments. We identified 612 differentially expressed genes in the embryos. These results confirm that the social environment does not only affect males but also the molecular ecology of their sperm and the gene expression in their offspring suggesting a putative role of sRNAs. Overall design: This data deposit includes the small RNA seq data for this research. Male zebrafish were subject to high and low social stress environments with sperm samples collected RNA extracted and sent for small RNA sequencing.   pubmed:40121340   La 10 GSM7916478   tissue:sperm|cell line:sperm|genotype:WT|treatment:Low1|geo loc name:missing|collection date:missing La 10 Reads were trimmed aligned with PatMan and then count matrices assembled before DESeq2 analyses Assembly: GRCz10 Supplementary files format and content: piRNA raw counts.csv raw counts of a subset of 24 out of the 40 samples that were analysed. These 24 were chosen due to noise observed in PCA clustering. Supplementary files format and content: mirna raw counts.csv raw counts for 39/40 samples as one sample failed to progress through our pipeline to the same high quality as the other 39 so this sample was omitted from analyses. sperm   Total RNA extraction New England BioLabs kit NEBNext®Multiplex Small RNA Library Prep Set for Illumina® Set 1 and 2 NEB #E7300 and NEB #E7580 small RNA library prep for Illumina   cell line:sperm|genotype:WT|treatment:Low1 GSM7916478 GSM7916478: La 10; Danio rerio; ncRNA Seq GSM7916478 r1 GSM7916478 1 Total RNA extraction New England BioLabs kit NEBNext®Multiplex Small RNA Library Prep Set for Illumina® Set 1 and 2 NEB #E7300 and NEB #E7580 small RNA library prep for Illumina   ncRNA-Seq TRANSCRIPTOMIC size fractionation SINGLE ILLUMINA Illumina HiSeq 2500   SRP473892     12235X3_160303_D00294_0224_AC95NJANXX_4.cutadapt.se.19_100.fastq.gz fastq 916186556.0 32659639.0 GSM7916478 r1 0:28.05 A:216314501;C:191651870;G:267047552;T:241092719;N:79914 28       216314501 191651870 267047552 241092719 79914 SRX22630071 SRS19628553 SRA1756943 University of East Anglia University of East Anglia 1 0.84312   0.23836   0.83132   0.57754   33   B   usable mapping rate illumina hiseq_era unknown size_fractionation nebnext bulk unknown unknown   United Kingdom 2023-11-23 Undetermined Embryo Cell Line Cell Line
29023 29023 SRR26936269 SRX22630070 SRS19628552 SRP473892 PRJNA1044439 Social stress in fathers affects sperm small RNA and offspring transcriptome profiles GSE248535 Transcriptome Analysis Environmental changes may affect paternal condition and following generations but the underlying mechanisms are poorly understood. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and development in their offspring. Here we investigated the role of sperm mediated small RNAs in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes and transposable elements TEs at this key developmental stage. We sequenced micro mi and Piwi interacting piRNAs in sperm and the full transcriptome in the embryos and ran differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from high to low competition environments. We identified 612 differentially expressed genes in the embryos. These results confirm that the social environment does not only affect males but also the molecular ecology of their sperm and the gene expression in their offspring suggesting a putative role of sRNAs. Overall design: This data deposit includes the small RNA seq data for this research. Male zebrafish were subject to high and low social stress environments with sperm samples collected RNA extracted and sent for small RNA sequencing.   pubmed:40121340   Ha 10 GSM7916477   tissue:sperm|cell line:sperm|genotype:WT|treatment:High1|geo loc name:missing|collection date:missing Ha 10 Reads were trimmed aligned with PatMan and then count matrices assembled before DESeq2 analyses Assembly: GRCz10 Supplementary files format and content: piRNA raw counts.csv raw counts of a subset of 24 out of the 40 samples that were analysed. These 24 were chosen due to noise observed in PCA clustering. Supplementary files format and content: mirna raw counts.csv raw counts for 39/40 samples as one sample failed to progress through our pipeline to the same high quality as the other 39 so this sample was omitted from analyses. sperm   Total RNA extraction New England BioLabs kit NEBNext®Multiplex Small RNA Library Prep Set for Illumina® Set 1 and 2 NEB #E7300 and NEB #E7580 small RNA library prep for Illumina   cell line:sperm|genotype:WT|treatment:High1 GSM7916477 GSM7916477: Ha 10; Danio rerio; ncRNA Seq GSM7916477 r1 GSM7916477 1 Total RNA extraction New England BioLabs kit NEBNext®Multiplex Small RNA Library Prep Set for Illumina® Set 1 and 2 NEB #E7300 and NEB #E7580 small RNA library prep for Illumina   ncRNA-Seq TRANSCRIPTOMIC size fractionation SINGLE ILLUMINA Illumina HiSeq 2500   SRP473892     12235X2_160303_D00294_0224_AC95NJANXX_4.cutadapt.se.19_100.fastq.gz fastq 858990742.0 30463457.0 GSM7916477 r1 0:28.20 A:182799146;C:179417249;G:265490509;T:231201694;N:82144 28       182799146 179417249 265490509 231201694 82144 SRX22630070 SRS19628552 SRA1756943 University of East Anglia University of East Anglia 1 0.67221   0.19137   0.87986   0.67831   33   B   usable mapping rate illumina hiseq_era unknown size_fractionation nebnext bulk unknown unknown   United Kingdom 2023-11-23 Undetermined Embryo Cell Line Cell Line
29024 29024 SRR26936270 SRX22630069 SRS19628551 SRP473892 PRJNA1044439 Social stress in fathers affects sperm small RNA and offspring transcriptome profiles GSE248535 Transcriptome Analysis Environmental changes may affect paternal condition and following generations but the underlying mechanisms are poorly understood. Male male competition induces a physiological stress response and affects male hormone levels ejaculate traits and development in their offspring. Here we investigated the role of sperm mediated small RNAs in the transmission of male condition to the next generation. We exposed male zebrafish Danio rerio to high and low male male competition environments for two weeks and collected sperm samples at the end. We also performed IVFs using a split clutch design to distinguish between paternal and maternal effects and collected embryos at 24 hours to test for differentially expressed genes and transposable elements TEs at this key developmental stage. We sequenced micro mi and Piwi interacting piRNAs in sperm and the full transcriptome in the embryos and ran differential expression analyses. We identified differentially expressed sperm mi and piRNAs with the strongest effects observed in sperm of males switching from high to low competition environments. We identified 612 differentially expressed genes in the embryos. These results confirm that the social environment does not only affect males but also the molecular ecology of their sperm and the gene expression in their offspring suggesting a putative role of sRNAs. Overall design: This data deposit includes the small RNA seq data for this research. Male zebrafish were subject to high and low social stress environments with sperm samples collected RNA extracted and sent for small RNA sequencing.   pubmed:40121340   Ha 2 GSM7916476   tissue:sperm|cell line:sperm|genotype:WT|treatment:High1|geo loc name:missing|collection date:missing Ha 2 Reads were trimmed aligned with PatMan and then count matrices assembled before DESeq2 analyses Assembly: GRCz10 Supplementary files format and content: piRNA raw counts.csv raw counts of a subset of 24 out of the 40 samples that were analysed. These 24 were chosen due to noise observed in PCA clustering. Supplementary files format and content: mirna raw counts.csv raw counts for 39/40 samples as one sample failed to progress through our pipeline to the same high quality as the other 39 so this sample was omitted from analyses. sperm   Total RNA extraction New England BioLabs kit NEBNext®Multiplex Small RNA Library Prep Set for Illumina® Set 1 and 2 NEB #E7300 and NEB #E7580 small RNA library prep for Illumina   cell line:sperm|genotype:WT|treatment:High1 GSM7916476 GSM7916476: Ha 2; Danio rerio; ncRNA Seq GSM7916476 r1 GSM7916476 1 Total RNA extraction New England BioLabs kit NEBNext®Multiplex Small RNA Library Prep Set for Illumina® Set 1 and 2 NEB #E7300 and NEB #E7580 small RNA library prep for Illumina   ncRNA-Seq TRANSCRIPTOMIC size fractionation SINGLE ILLUMINA Illumina HiSeq 2500   SRP473892     12235X1_160303_D00294_0224_AC95NJANXX_4.cutadapt.se.19_100.fastq.gz fastq 262855265.0 9317037.0 GSM7916476 r1 0:28.21 A:59555006;C:56655912;G:79385169;T:67236972;N:22206 28       59555006 56655912 79385169 67236972 22206 SRX22630069 SRS19628551 SRA1756943 University of East Anglia University of East Anglia 1 0.85088   0.21543   0.84102   0.63415   28   B   usable mapping rate illumina hiseq_era unknown size_fractionation nebnext bulk unknown unknown   United Kingdom 2023-11-23 Undetermined Embryo Cell Line Cell Line
44943 44943 SRR6324387 SRX3424140 SRS2715772 SRP125677 PRJNA419909 Characterization of lncRNAs involved in cold acclimation of zebrafish ZF4 cells GSE107369 Transcriptome Analysis Long non coding RNAs lncRNAs are increasingly regarded as a key role in regulating diverse biological processes in various tissues and species. So far no data is available on screening and functional prediction of lncRNAs in cold acclimation in fishes. Here we compared expression profiles of lncRNAs in zebrafish embryonic fibroblast cells ZF4 cultured at 28°C as control with that of cold acclimated cells cultured at 18°C for 30 days by high throughput sequencing. There are 8363 novel lncRNAs were identified. Including known and novel lncRNAs there are 347 lncRNAs up regulated and 342 lncRNAs down regulated in cold acclimated cells compared with that of control cells at 28°C. Among differentially expressed lncRNAs 74 and 61 were found only in control cells or in cold acclimated cells respectively. The Gene Ontology GO and Kyoto Encyclopaedia of Genes and Genomes KEGG enrichment analyses of genes adjacent to differentially expressed lncRNAs showed that enriched genes were involved in electron transport cell adhesion oxidation reduction process and so on. We predicted target genes of differentially expressed lncRNAs by looking for interactions between lncRNAs and mRNA and constructed a complex interaction network. In summary our genome wide systematic identification and function prediction of lncRNAs in zebrafish cells lays the foundation for future studies to elucidate crucial roles of lncRNAs in cold acclimation in fishes. Overall design: ZF4 cells were cultured at 28 °C as control and at 18 °C for 30 days seperately. Each condition has three biological replica.   pubmed:29634734   ZF4 18 30d rep3 GSM2865571   tissue:ZF4 18 30d|cell line:ZF4|cell type:embryonic fibroblast cell line|cultured at:18°C for 30 days|sample group:cold treatment for 30 days ZF4 18 30d rep3 Illumina Casava1.8 software used for basecalling. Clean RNA seq reads for each sample were aligned by HISAT2 2.1.0 with default setting to the zebrafish genome assembly GRCz10 Transcripts were assembled by StringTie 1.3.3 with parameter “ G Danio rerio.GRCz10.88.gtf” all assemblies were merged together utilizing StringTie’s “merge” function CPC Coding Potential Calculator CNCI Coding Non Coding Index and Pfam were used to delete transcripts with coding potential The genes expression levels were estimated by FPKM fragments per kilobase of exon per million fragments mapped and assessed using StringTie Genome build: GRCz10 Supplementary files format and content: tab delimited text files include FPKM values for novel lncRNAs ZF4 18 30d For cold treatment cells were grown at 18 °C 5% CO2 in the same medium for up to 30 days. Total RNA was extracted using miRNeasy Mini Kit 217004 Qiagen and purified by RNAClean XP Kit A63987 Beckman Coulter and RNase Free DNase Set 79254 Qiagen. Libraries were constructed using the TruSeq Stranded Total RNA LT Sample Prep Kit with Ribo Zero RS 122 2301/ RS 122 2302 Illumina. Libraries were pooled and sequenced using the Illumina HiSeq machine as 150 bp paired end sequencing reads. The zebrafish embryonic fibroblast cell line ZF4 was purchased from the American Type Culture Collection ATCC CRL 2050; Driever and Rangini 1993. The cells were grown at 28 °C 5% CO2 in Dulbecco's modified Eagle's medium/F12 nutrient mix DMEM/F12 supplemented with 10% FBS 1% l glutamine 100 u/ml penicillin 100 μg/ml streptomycin. For cold treatment cells were grown at 18 °C 5% CO2 in the same medium for up to 30 days. All cell culture medium components were purchased from Gibco BRL Life Technologies Ltd USA. cell line:ZF4|cell type:embryonic fibroblast cell line|cultured at:18°C for 30 days|sample group:cold treatment for 30 days GSM2865571 GSM2865571: ZF4 18 30d rep3; Danio rerio; RNA Seq GSM2865571   1 Total RNA was extracted using miRNeasy Mini Kit 217004 Qiagen and purified by RNAClean XP Kit A63987 Beckman Coulter and RNase Free DNase Set 79254 Qiagen. Libraries were constructed using the TruSeq Stranded Total RNA LT Sample Prep Kit with Ribo Zero RS 122 2301/ RS 122 2302 Illumina. Libraries were pooled and sequenced using the Illumina HiSeq machine as 150 bp paired end sequencing reads. GEO Accession:GSM2865571 RNA-Seq TRANSCRIPTOMIC cDNA PAIRED ILLUMINA HiSeq X Ten   SRP125677     6_1.fq.gz 6_2.fq.gz fastq fastq 8502006300.0 28340021.0 GSM2865571 r1 0:150 1:150 A:2222321266;C:2022701531;G:2022825678;T:2234014077;N:143748 150 150     2222321266 2022701531 2022825678 2234014077 143748 SRX3424140 SRS2715772 SRA634103 GEO Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, College of Fisheries and Life Science, Shanghai Ocean University 2 0.92868 0.92895 0.15866 0.15599 0.74146 0.74505 0.49577 0.49894 150 150 B B biological fallback assumption illumina hiseq_era unknown rrna_depletion ribozero bulk unknown unknown   China 2017-11-27 Undetermined Embryo Cell Line Cell Line
44944 44944 SRR6324386 SRX3424139 SRS2715771 SRP125677 PRJNA419909 Characterization of lncRNAs involved in cold acclimation of zebrafish ZF4 cells GSE107369 Transcriptome Analysis Long non coding RNAs lncRNAs are increasingly regarded as a key role in regulating diverse biological processes in various tissues and species. So far no data is available on screening and functional prediction of lncRNAs in cold acclimation in fishes. Here we compared expression profiles of lncRNAs in zebrafish embryonic fibroblast cells ZF4 cultured at 28°C as control with that of cold acclimated cells cultured at 18°C for 30 days by high throughput sequencing. There are 8363 novel lncRNAs were identified. Including known and novel lncRNAs there are 347 lncRNAs up regulated and 342 lncRNAs down regulated in cold acclimated cells compared with that of control cells at 28°C. Among differentially expressed lncRNAs 74 and 61 were found only in control cells or in cold acclimated cells respectively. The Gene Ontology GO and Kyoto Encyclopaedia of Genes and Genomes KEGG enrichment analyses of genes adjacent to differentially expressed lncRNAs showed that enriched genes were involved in electron transport cell adhesion oxidation reduction process and so on. We predicted target genes of differentially expressed lncRNAs by looking for interactions between lncRNAs and mRNA and constructed a complex interaction network. In summary our genome wide systematic identification and function prediction of lncRNAs in zebrafish cells lays the foundation for future studies to elucidate crucial roles of lncRNAs in cold acclimation in fishes. Overall design: ZF4 cells were cultured at 28 °C as control and at 18 °C for 30 days seperately. Each condition has three biological replica.   pubmed:29634734   ZF4 18 30d rep2 GSM2865570   tissue:ZF4 18 30d|cell line:ZF4|cell type:embryonic fibroblast cell line|cultured at:18°C for 30 days|sample group:cold treatment for 30 days ZF4 18 30d rep2 Illumina Casava1.8 software used for basecalling. Clean RNA seq reads for each sample were aligned by HISAT2 2.1.0 with default setting to the zebrafish genome assembly GRCz10 Transcripts were assembled by StringTie 1.3.3 with parameter “ G Danio rerio.GRCz10.88.gtf” all assemblies were merged together utilizing StringTie’s “merge” function CPC Coding Potential Calculator CNCI Coding Non Coding Index and Pfam were used to delete transcripts with coding potential The genes expression levels were estimated by FPKM fragments per kilobase of exon per million fragments mapped and assessed using StringTie Genome build: GRCz10 Supplementary files format and content: tab delimited text files include FPKM values for novel lncRNAs ZF4 18 30d For cold treatment cells were grown at 18 °C 5% CO2 in the same medium for up to 30 days. Total RNA was extracted using miRNeasy Mini Kit 217004 Qiagen and purified by RNAClean XP Kit A63987 Beckman Coulter and RNase Free DNase Set 79254 Qiagen. Libraries were constructed using the TruSeq Stranded Total RNA LT Sample Prep Kit with Ribo Zero RS 122 2301/ RS 122 2302 Illumina. Libraries were pooled and sequenced using the Illumina HiSeq machine as 150 bp paired end sequencing reads. The zebrafish embryonic fibroblast cell line ZF4 was purchased from the American Type Culture Collection ATCC CRL 2050; Driever and Rangini 1993. The cells were grown at 28 °C 5% CO2 in Dulbecco's modified Eagle's medium/F12 nutrient mix DMEM/F12 supplemented with 10% FBS 1% l glutamine 100 u/ml penicillin 100 μg/ml streptomycin. For cold treatment cells were grown at 18 °C 5% CO2 in the same medium for up to 30 days. All cell culture medium components were purchased from Gibco BRL Life Technologies Ltd USA. cell line:ZF4|cell type:embryonic fibroblast cell line|cultured at:18°C for 30 days|sample group:cold treatment for 30 days GSM2865570 GSM2865570: ZF4 18 30d rep2; Danio rerio; RNA Seq GSM2865570   1 Total RNA was extracted using miRNeasy Mini Kit 217004 Qiagen and purified by RNAClean XP Kit A63987 Beckman Coulter and RNase Free DNase Set 79254 Qiagen. Libraries were constructed using the TruSeq Stranded Total RNA LT Sample Prep Kit with Ribo Zero RS 122 2301/ RS 122 2302 Illumina. Libraries were pooled and sequenced using the Illumina HiSeq machine as 150 bp paired end sequencing reads. GEO Accession:GSM2865570 RNA-Seq TRANSCRIPTOMIC cDNA PAIRED ILLUMINA HiSeq X Ten   SRP125677     4_1.fq.gz 4_2.fq.gz fastq fastq 8571202200.0 28570674.0 GSM2865570 r1 0:150 1:150 A:2268978542;C:2005726985;G:2030919273;T:2265431269;N:146131 150 150     2268978542 2005726985 2030919273 2265431269 146131 SRX3424139 SRS2715771 SRA634103 GEO Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, College of Fisheries and Life Science, Shanghai Ocean University 2 0.92516 0.92417 0.18269 0.17989 0.73352 0.73813 0.51554 0.50598 150 150 B B biological fallback assumption illumina hiseq_era unknown rrna_depletion ribozero bulk unknown unknown   China 2017-11-27 Undetermined Embryo Cell Line Cell Line
44945 44945 SRR6324385 SRX3424138 SRS2715770 SRP125677 PRJNA419909 Characterization of lncRNAs involved in cold acclimation of zebrafish ZF4 cells GSE107369 Transcriptome Analysis Long non coding RNAs lncRNAs are increasingly regarded as a key role in regulating diverse biological processes in various tissues and species. So far no data is available on screening and functional prediction of lncRNAs in cold acclimation in fishes. Here we compared expression profiles of lncRNAs in zebrafish embryonic fibroblast cells ZF4 cultured at 28°C as control with that of cold acclimated cells cultured at 18°C for 30 days by high throughput sequencing. There are 8363 novel lncRNAs were identified. Including known and novel lncRNAs there are 347 lncRNAs up regulated and 342 lncRNAs down regulated in cold acclimated cells compared with that of control cells at 28°C. Among differentially expressed lncRNAs 74 and 61 were found only in control cells or in cold acclimated cells respectively. The Gene Ontology GO and Kyoto Encyclopaedia of Genes and Genomes KEGG enrichment analyses of genes adjacent to differentially expressed lncRNAs showed that enriched genes were involved in electron transport cell adhesion oxidation reduction process and so on. We predicted target genes of differentially expressed lncRNAs by looking for interactions between lncRNAs and mRNA and constructed a complex interaction network. In summary our genome wide systematic identification and function prediction of lncRNAs in zebrafish cells lays the foundation for future studies to elucidate crucial roles of lncRNAs in cold acclimation in fishes. Overall design: ZF4 cells were cultured at 28 °C as control and at 18 °C for 30 days seperately. Each condition has three biological replica.   pubmed:29634734   ZF4 18 30d rep1 GSM2865569   tissue:ZF4 18 30d|cell line:ZF4|cell type:embryonic fibroblast cell line|cultured at:18°C for 30 days|sample group:cold treatment for 30 days ZF4 18 30d rep1 Illumina Casava1.8 software used for basecalling. Clean RNA seq reads for each sample were aligned by HISAT2 2.1.0 with default setting to the zebrafish genome assembly GRCz10 Transcripts were assembled by StringTie 1.3.3 with parameter “ G Danio rerio.GRCz10.88.gtf” all assemblies were merged together utilizing StringTie’s “merge” function CPC Coding Potential Calculator CNCI Coding Non Coding Index and Pfam were used to delete transcripts with coding potential The genes expression levels were estimated by FPKM fragments per kilobase of exon per million fragments mapped and assessed using StringTie Genome build: GRCz10 Supplementary files format and content: tab delimited text files include FPKM values for novel lncRNAs ZF4 18 30d For cold treatment cells were grown at 18 °C 5% CO2 in the same medium for up to 30 days. Total RNA was extracted using miRNeasy Mini Kit 217004 Qiagen and purified by RNAClean XP Kit A63987 Beckman Coulter and RNase Free DNase Set 79254 Qiagen. Libraries were constructed using the TruSeq Stranded Total RNA LT Sample Prep Kit with Ribo Zero RS 122 2301/ RS 122 2302 Illumina. Libraries were pooled and sequenced using the Illumina HiSeq machine as 150 bp paired end sequencing reads. The zebrafish embryonic fibroblast cell line ZF4 was purchased from the American Type Culture Collection ATCC CRL 2050; Driever and Rangini 1993. The cells were grown at 28 °C 5% CO2 in Dulbecco's modified Eagle's medium/F12 nutrient mix DMEM/F12 supplemented with 10% FBS 1% l glutamine 100 u/ml penicillin 100 μg/ml streptomycin. For cold treatment cells were grown at 18 °C 5% CO2 in the same medium for up to 30 days. All cell culture medium components were purchased from Gibco BRL Life Technologies Ltd USA. cell line:ZF4|cell type:embryonic fibroblast cell line|cultured at:18°C for 30 days|sample group:cold treatment for 30 days GSM2865569 GSM2865569: ZF4 18 30d rep1; Danio rerio; RNA Seq GSM2865569   1 Total RNA was extracted using miRNeasy Mini Kit 217004 Qiagen and purified by RNAClean XP Kit A63987 Beckman Coulter and RNase Free DNase Set 79254 Qiagen. Libraries were constructed using the TruSeq Stranded Total RNA LT Sample Prep Kit with Ribo Zero RS 122 2301/ RS 122 2302 Illumina. Libraries were pooled and sequenced using the Illumina HiSeq machine as 150 bp paired end sequencing reads. GEO Accession:GSM2865569 RNA-Seq TRANSCRIPTOMIC cDNA PAIRED ILLUMINA HiSeq X Ten   SRP125677     2_1.fq.gz 2_2.fq.gz fastq fastq 9855530100.0 32851767.0 GSM2865569 r1 0:150 1:150 A:2584345651;C:2339107553;G:2359863664;T:2572047288;N:165944 150 150     2584345651 2339107553 2359863664 2572047288 165944 SRX3424138 SRS2715770 SRA634103 GEO Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, College of Fisheries and Life Science, Shanghai Ocean University 2 0.92395 0.92354 0.18307 0.17869 0.73667 0.74199 0.52681 0.53689 150 150 B B biological fallback assumption illumina hiseq_era unknown rrna_depletion ribozero bulk unknown unknown   China 2017-11-27 Undetermined Embryo Cell Line Cell Line
44946 44946 SRR6324384 SRX3424137 SRS2715768 SRP125677 PRJNA419909 Characterization of lncRNAs involved in cold acclimation of zebrafish ZF4 cells GSE107369 Transcriptome Analysis Long non coding RNAs lncRNAs are increasingly regarded as a key role in regulating diverse biological processes in various tissues and species. So far no data is available on screening and functional prediction of lncRNAs in cold acclimation in fishes. Here we compared expression profiles of lncRNAs in zebrafish embryonic fibroblast cells ZF4 cultured at 28°C as control with that of cold acclimated cells cultured at 18°C for 30 days by high throughput sequencing. There are 8363 novel lncRNAs were identified. Including known and novel lncRNAs there are 347 lncRNAs up regulated and 342 lncRNAs down regulated in cold acclimated cells compared with that of control cells at 28°C. Among differentially expressed lncRNAs 74 and 61 were found only in control cells or in cold acclimated cells respectively. The Gene Ontology GO and Kyoto Encyclopaedia of Genes and Genomes KEGG enrichment analyses of genes adjacent to differentially expressed lncRNAs showed that enriched genes were involved in electron transport cell adhesion oxidation reduction process and so on. We predicted target genes of differentially expressed lncRNAs by looking for interactions between lncRNAs and mRNA and constructed a complex interaction network. In summary our genome wide systematic identification and function prediction of lncRNAs in zebrafish cells lays the foundation for future studies to elucidate crucial roles of lncRNAs in cold acclimation in fishes. Overall design: ZF4 cells were cultured at 28 °C as control and at 18 °C for 30 days seperately. Each condition has three biological replica.   pubmed:29634734   ZF4 28 rep3 GSM2865568   tissue:ZF4 28|cell line:ZF4|cell type:embryonic fibroblast cell line|cultured at:normal condition 28°C|sample group:control ZF4 28 rep3 Illumina Casava1.8 software used for basecalling. Clean RNA seq reads for each sample were aligned by HISAT2 2.1.0 with default setting to the zebrafish genome assembly GRCz10 Transcripts were assembled by StringTie 1.3.3 with parameter “ G Danio rerio.GRCz10.88.gtf” all assemblies were merged together utilizing StringTie’s “merge” function CPC Coding Potential Calculator CNCI Coding Non Coding Index and Pfam were used to delete transcripts with coding potential The genes expression levels were estimated by FPKM fragments per kilobase of exon per million fragments mapped and assessed using StringTie Genome build: GRCz10 Supplementary files format and content: tab delimited text files include FPKM values for novel lncRNAs ZF4 28 For cold treatment cells were grown at 18 °C 5% CO2 in the same medium for up to 30 days. Total RNA was extracted using miRNeasy Mini Kit 217004 Qiagen and purified by RNAClean XP Kit A63987 Beckman Coulter and RNase Free DNase Set 79254 Qiagen. Libraries were constructed using the TruSeq Stranded Total RNA LT Sample Prep Kit with Ribo Zero RS 122 2301/ RS 122 2302 Illumina. Libraries were pooled and sequenced using the Illumina HiSeq machine as 150 bp paired end sequencing reads. The zebrafish embryonic fibroblast cell line ZF4 was purchased from the American Type Culture Collection ATCC CRL 2050; Driever and Rangini 1993. The cells were grown at 28 °C 5% CO2 in Dulbecco's modified Eagle's medium/F12 nutrient mix DMEM/F12 supplemented with 10% FBS 1% l glutamine 100 u/ml penicillin 100 μg/ml streptomycin. For cold treatment cells were grown at 18 °C 5% CO2 in the same medium for up to 30 days. All cell culture medium components were purchased from Gibco BRL Life Technologies Ltd USA. cell line:ZF4|cell type:embryonic fibroblast cell line|cultured at:normal condition 28°C|sample group:control GSM2865568 GSM2865568: ZF4 28 rep3; Danio rerio; RNA Seq GSM2865568   1 Total RNA was extracted using miRNeasy Mini Kit 217004 Qiagen and purified by RNAClean XP Kit A63987 Beckman Coulter and RNase Free DNase Set 79254 Qiagen. Libraries were constructed using the TruSeq Stranded Total RNA LT Sample Prep Kit with Ribo Zero RS 122 2301/ RS 122 2302 Illumina. Libraries were pooled and sequenced using the Illumina HiSeq machine as 150 bp paired end sequencing reads. GEO Accession:GSM2865568 RNA-Seq TRANSCRIPTOMIC cDNA PAIRED ILLUMINA HiSeq X Ten   SRP125677     5_1.fq.gz 5_2.fq.gz fastq fastq 6344338200.0 21147794.0 GSM2865568 r1 0:150 1:150 A:1726699573;C:1445357744;G:1438849187;T:1733324130;N:107566 150 150     1726699573 1445357744 1438849187 1733324130 107566 SRX3424137 SRS2715768 SRA634103 GEO Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, College of Fisheries and Life Science, Shanghai Ocean University 2 0.9124 0.91371 0.29012 0.28803 0.73525 0.74038 0.53934 0.55133 150 150 B B biological fallback assumption illumina hiseq_era unknown rrna_depletion ribozero bulk unknown unknown   China 2017-11-27 Undetermined Embryo Cell Line Cell Line
44947 44947 SRR6324383 SRX3424136 SRS2715767 SRP125677 PRJNA419909 Characterization of lncRNAs involved in cold acclimation of zebrafish ZF4 cells GSE107369 Transcriptome Analysis Long non coding RNAs lncRNAs are increasingly regarded as a key role in regulating diverse biological processes in various tissues and species. So far no data is available on screening and functional prediction of lncRNAs in cold acclimation in fishes. Here we compared expression profiles of lncRNAs in zebrafish embryonic fibroblast cells ZF4 cultured at 28°C as control with that of cold acclimated cells cultured at 18°C for 30 days by high throughput sequencing. There are 8363 novel lncRNAs were identified. Including known and novel lncRNAs there are 347 lncRNAs up regulated and 342 lncRNAs down regulated in cold acclimated cells compared with that of control cells at 28°C. Among differentially expressed lncRNAs 74 and 61 were found only in control cells or in cold acclimated cells respectively. The Gene Ontology GO and Kyoto Encyclopaedia of Genes and Genomes KEGG enrichment analyses of genes adjacent to differentially expressed lncRNAs showed that enriched genes were involved in electron transport cell adhesion oxidation reduction process and so on. We predicted target genes of differentially expressed lncRNAs by looking for interactions between lncRNAs and mRNA and constructed a complex interaction network. In summary our genome wide systematic identification and function prediction of lncRNAs in zebrafish cells lays the foundation for future studies to elucidate crucial roles of lncRNAs in cold acclimation in fishes. Overall design: ZF4 cells were cultured at 28 °C as control and at 18 °C for 30 days seperately. Each condition has three biological replica.   pubmed:29634734   ZF4 28 rep2 GSM2865567   tissue:ZF4 28|cell line:ZF4|cell type:embryonic fibroblast cell line|cultured at:normal condition 28°C|sample group:control ZF4 28 rep2 Illumina Casava1.8 software used for basecalling. Clean RNA seq reads for each sample were aligned by HISAT2 2.1.0 with default setting to the zebrafish genome assembly GRCz10 Transcripts were assembled by StringTie 1.3.3 with parameter “ G Danio rerio.GRCz10.88.gtf” all assemblies were merged together utilizing StringTie’s “merge” function CPC Coding Potential Calculator CNCI Coding Non Coding Index and Pfam were used to delete transcripts with coding potential The genes expression levels were estimated by FPKM fragments per kilobase of exon per million fragments mapped and assessed using StringTie Genome build: GRCz10 Supplementary files format and content: tab delimited text files include FPKM values for novel lncRNAs ZF4 28 For cold treatment cells were grown at 18 °C 5% CO2 in the same medium for up to 30 days. Total RNA was extracted using miRNeasy Mini Kit 217004 Qiagen and purified by RNAClean XP Kit A63987 Beckman Coulter and RNase Free DNase Set 79254 Qiagen. Libraries were constructed using the TruSeq Stranded Total RNA LT Sample Prep Kit with Ribo Zero RS 122 2301/ RS 122 2302 Illumina. Libraries were pooled and sequenced using the Illumina HiSeq machine as 150 bp paired end sequencing reads. The zebrafish embryonic fibroblast cell line ZF4 was purchased from the American Type Culture Collection ATCC CRL 2050; Driever and Rangini 1993. The cells were grown at 28 °C 5% CO2 in Dulbecco's modified Eagle's medium/F12 nutrient mix DMEM/F12 supplemented with 10% FBS 1% l glutamine 100 u/ml penicillin 100 μg/ml streptomycin. For cold treatment cells were grown at 18 °C 5% CO2 in the same medium for up to 30 days. All cell culture medium components were purchased from Gibco BRL Life Technologies Ltd USA. cell line:ZF4|cell type:embryonic fibroblast cell line|cultured at:normal condition 28°C|sample group:control GSM2865567 GSM2865567: ZF4 28 rep2; Danio rerio; RNA Seq GSM2865567   1 Total RNA was extracted using miRNeasy Mini Kit 217004 Qiagen and purified by RNAClean XP Kit A63987 Beckman Coulter and RNase Free DNase Set 79254 Qiagen. Libraries were constructed using the TruSeq Stranded Total RNA LT Sample Prep Kit with Ribo Zero RS 122 2301/ RS 122 2302 Illumina. Libraries were pooled and sequenced using the Illumina HiSeq machine as 150 bp paired end sequencing reads. GEO Accession:GSM2865567 RNA-Seq TRANSCRIPTOMIC cDNA PAIRED ILLUMINA HiSeq X Ten   SRP125677     3_1.fq.gz 3_2.fq.gz fastq fastq 7909395600.0 26364652.0 GSM2865567 r1 0:150 1:150 A:2096969069;C:1849259032;G:1867407953;T:2095652151;N:107395 150 150     2096969069 1849259032 1867407953 2095652151 107395 SRX3424136 SRS2715767 SRA634103 GEO Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, College of Fisheries and Life Science, Shanghai Ocean University 2 0.90044 0.89889 0.19795 0.1953 0.73898 0.74182 0.5238 0.52236 150 150 B B biological fallback assumption illumina hiseq_era unknown rrna_depletion ribozero bulk unknown unknown   China 2017-11-27 Undetermined Embryo Cell Line Cell Line
44948 44948 SRR6324382 SRX3424135 SRS2715769 SRP125677 PRJNA419909 Characterization of lncRNAs involved in cold acclimation of zebrafish ZF4 cells GSE107369 Transcriptome Analysis Long non coding RNAs lncRNAs are increasingly regarded as a key role in regulating diverse biological processes in various tissues and species. So far no data is available on screening and functional prediction of lncRNAs in cold acclimation in fishes. Here we compared expression profiles of lncRNAs in zebrafish embryonic fibroblast cells ZF4 cultured at 28°C as control with that of cold acclimated cells cultured at 18°C for 30 days by high throughput sequencing. There are 8363 novel lncRNAs were identified. Including known and novel lncRNAs there are 347 lncRNAs up regulated and 342 lncRNAs down regulated in cold acclimated cells compared with that of control cells at 28°C. Among differentially expressed lncRNAs 74 and 61 were found only in control cells or in cold acclimated cells respectively. The Gene Ontology GO and Kyoto Encyclopaedia of Genes and Genomes KEGG enrichment analyses of genes adjacent to differentially expressed lncRNAs showed that enriched genes were involved in electron transport cell adhesion oxidation reduction process and so on. We predicted target genes of differentially expressed lncRNAs by looking for interactions between lncRNAs and mRNA and constructed a complex interaction network. In summary our genome wide systematic identification and function prediction of lncRNAs in zebrafish cells lays the foundation for future studies to elucidate crucial roles of lncRNAs in cold acclimation in fishes. Overall design: ZF4 cells were cultured at 28 °C as control and at 18 °C for 30 days seperately. Each condition has three biological replica.   pubmed:29634734   ZF4 28 rep1 GSM2865566   tissue:ZF4 28|cell line:ZF4|cell type:embryonic fibroblast cell line|cultured at:normal condition 28°C|sample group:control ZF4 28 rep1 Illumina Casava1.8 software used for basecalling. Clean RNA seq reads for each sample were aligned by HISAT2 2.1.0 with default setting to the zebrafish genome assembly GRCz10 Transcripts were assembled by StringTie 1.3.3 with parameter “ G Danio rerio.GRCz10.88.gtf” all assemblies were merged together utilizing StringTie’s “merge” function CPC Coding Potential Calculator CNCI Coding Non Coding Index and Pfam were used to delete transcripts with coding potential The genes expression levels were estimated by FPKM fragments per kilobase of exon per million fragments mapped and assessed using StringTie Genome build: GRCz10 Supplementary files format and content: tab delimited text files include FPKM values for novel lncRNAs ZF4 28 For cold treatment cells were grown at 18 °C 5% CO2 in the same medium for up to 30 days. Total RNA was extracted using miRNeasy Mini Kit 217004 Qiagen and purified by RNAClean XP Kit A63987 Beckman Coulter and RNase Free DNase Set 79254 Qiagen. Libraries were constructed using the TruSeq Stranded Total RNA LT Sample Prep Kit with Ribo Zero RS 122 2301/ RS 122 2302 Illumina. Libraries were pooled and sequenced using the Illumina HiSeq machine as 150 bp paired end sequencing reads. The zebrafish embryonic fibroblast cell line ZF4 was purchased from the American Type Culture Collection ATCC CRL 2050; Driever and Rangini 1993. The cells were grown at 28 °C 5% CO2 in Dulbecco's modified Eagle's medium/F12 nutrient mix DMEM/F12 supplemented with 10% FBS 1% l glutamine 100 u/ml penicillin 100 μg/ml streptomycin. For cold treatment cells were grown at 18 °C 5% CO2 in the same medium for up to 30 days. All cell culture medium components were purchased from Gibco BRL Life Technologies Ltd USA. cell line:ZF4|cell type:embryonic fibroblast cell line|cultured at:normal condition 28°C|sample group:control GSM2865566 GSM2865566: ZF4 28 rep1; Danio rerio; RNA Seq GSM2865566   1 Total RNA was extracted using miRNeasy Mini Kit 217004 Qiagen and purified by RNAClean XP Kit A63987 Beckman Coulter and RNase Free DNase Set 79254 Qiagen. Libraries were constructed using the TruSeq Stranded Total RNA LT Sample Prep Kit with Ribo Zero RS 122 2301/ RS 122 2302 Illumina. Libraries were pooled and sequenced using the Illumina HiSeq machine as 150 bp paired end sequencing reads. GEO Accession:GSM2865566 RNA-Seq TRANSCRIPTOMIC cDNA PAIRED ILLUMINA HiSeq X Ten   SRP125677     1_2.fq.gz 1_1.fq.gz fastq fastq 9364351500.0 31214505.0 GSM2865566 r1 0:150 1:150 A:2483908747;C:2191875885;G:2204013829;T:2484392803;N:160236 150 150     2483908747 2191875885 2204013829 2484392803 160236 SRX3424135 SRS2715769 SRA634103 GEO Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, College of Fisheries and Life Science, Shanghai Ocean University 2 0.91474 0.91441 0.21056 0.20796 0.739 0.74477 0.52778 0.53276 150 150 B B biological fallback assumption illumina hiseq_era unknown rrna_depletion ribozero bulk unknown unknown   China 2017-11-27 Undetermined Embryo Cell Line Cell Line
49815 49815 SRR8987978 SRX5767074 SRS4701368 SRP166817 PRJNA498018 Danio rerio strain:ZFL cell line Genome sequencing PRJNA498018 Whole Genome Sequencing Transcriptional responses of ZFL exposed to microcystin LR       24h 6 sample exposed to microcystin for xxxh replicate #6   ecotype:not applicable|dev stage:not applicable|sex:not applicable|tissue:ZFL cell line|cell line:ZFL cell line|treatment:sample exposed to microcystin for xxxh replicate #6|BioSampleModel:Model organism or animal                 sample exposed to microcystin for xxxh replicate #6 24h 3 24h 3 Sequencing libraries were generated using the TruSeq RNA Sample Preparation Kit Illumina according to manufacturers instructions. Three micrograms of total RNA were used as initial material for library preparation. Briefly mRNA was purified using poly T oligo attached magnetic beads. Fragmentation of mRNA was conducted using divalent cations under elevated temperature in the Illumina proprietary fragmentation buffer. First strand cDNA was synthesized using random oligonucleotides and SuperScript II. Second strand cDNA was subsequently synthesized using DNA Polymerase I and RNase H. post adenylation of the 3 ends of DNA fragments Illumina PE adapter oligonucleotides were ligated and the library fragments were purified using the AMPure XP system Beckman Coulter. DNA fragments with ligated adaptors on both ends were selectively enriched using Illumina PCR Primer Cocktail in a 15 cycle PCR reaction. The products were purified AMPure XP system and quantified using the Agilent high sensitivity DNA assay on a Bioanalyzer 2100 system Agilent. The sequencing libraries were then sequenced at both ends for 150 bp using a Hiseq Xten platform Illumina by Shanghai Personal Biotechnology Cp. Ltd.     RNA-Seq TRANSCRIPTOMIC PCR PAIRED ILLUMINA HiSeq X Ten   SRP166817     24h3_clean_R2.fq.gz 24h3_clean_R1.fq.gz fastq fastq 6275458528.0 20779664.0 24h3 clean R1.fq.gz 0:151 1:151 A:1685303886;C:1437291030;G:1447147160;T:1702856463;N:2859989 151 151     1685303886 1437291030 1447147160 1702856463 2859989 SRX5767074 SRS4701368 SRA880742 Chinese Academy of Fishery Sciences|Yangtze River Fisheries Research Institute Chinese Academy of Fishery Sciences 2 0.92675 0.92679 0.13415 0.13431 0.73945 0.74213 0.50477 0.50726 151 151 B B biological fallback assumption illumina hiseq_era unknown random_priming trueseq bulk unknown unknown   China 2019-04-29 Undetermined Undetermined Cell Line Cell Line
49816 49816 SRR8987979 SRX5767073 SRS4701367 SRP166817 PRJNA498018 Danio rerio strain:ZFL cell line Genome sequencing PRJNA498018 Whole Genome Sequencing Transcriptional responses of ZFL exposed to microcystin LR       24h 4 sample exposed to microcystin for xxxh replicate #4   ecotype:not applicable|dev stage:not applicable|sex:not applicable|tissue:ZFL cell line|cell line:ZFL cell line|treatment:sample exposed to microcystin for xxxh replicate #4|BioSampleModel:Model organism or animal                 sample exposed to microcystin for xxxh replicate #4 24h 1 24h 1 Sequencing libraries were generated using the TruSeq RNA Sample Preparation Kit Illumina according to manufacturers instructions. Three micrograms of total RNA were used as initial material for library preparation. Briefly mRNA was purified using poly T oligo attached magnetic beads. Fragmentation of mRNA was conducted using divalent cations under elevated temperature in the Illumina proprietary fragmentation buffer. First strand cDNA was synthesized using random oligonucleotides and SuperScript II. Second strand cDNA was subsequently synthesized using DNA Polymerase I and RNase H. post adenylation of the 3 ends of DNA fragments Illumina PE adapter oligonucleotides were ligated and the library fragments were purified using the AMPure XP system Beckman Coulter. DNA fragments with ligated adaptors on both ends were selectively enriched using Illumina PCR Primer Cocktail in a 15 cycle PCR reaction. The products were purified AMPure XP system and quantified using the Agilent high sensitivity DNA assay on a Bioanalyzer 2100 system Agilent. The sequencing libraries were then sequenced at both ends for 150 bp using a Hiseq Xten platform Illumina by Shanghai Personal Biotechnology Cp. Ltd.     RNA-Seq TRANSCRIPTOMIC PCR PAIRED ILLUMINA HiSeq X Ten   SRP166817     24h1_clean_R1.fq.gz 24h1_clean_R2.fq.gz fastq fastq 6373552356.0 21104478.0 24h1 clean R1.fq.gz 0:151 1:151 A:1712827186;C:1458408871;G:1470508604;T:1728973011;N:2834684 151 151     1712827186 1458408871 1470508604 1728973011 2834684 SRX5767073 SRS4701367 SRA880742 Chinese Academy of Fishery Sciences|Yangtze River Fisheries Research Institute Chinese Academy of Fishery Sciences 2 0.9248 0.92582 0.13207 0.13239 0.73839 0.74038 0.49865 0.50119 151 151 B B biological fallback assumption illumina hiseq_era unknown random_priming trueseq bulk unknown unknown   China 2019-04-29 Undetermined Undetermined Cell Line Cell Line
49817 49817 SRR8987980 SRX5767072 SRS4701366 SRP166817 PRJNA498018 Danio rerio strain:ZFL cell line Genome sequencing PRJNA498018 Whole Genome Sequencing Transcriptional responses of ZFL exposed to microcystin LR       24h 5 sample exposed to microcystin for xxxh replicate #5   ecotype:not applicable|dev stage:not applicable|sex:not applicable|tissue:ZFL cell line|cell line:ZFL cell line|treatment:sample exposed to microcystin for xxxh replicate #5|BioSampleModel:Model organism or animal                 sample exposed to microcystin for xxxh replicate #5 24h 2 24h 2 Sequencing libraries were generated using the TruSeq RNA Sample Preparation Kit Illumina according to manufacturers instructions. Three micrograms of total RNA were used as initial material for library preparation. Briefly mRNA was purified using poly T oligo attached magnetic beads. Fragmentation of mRNA was conducted using divalent cations under elevated temperature in the Illumina proprietary fragmentation buffer. First strand cDNA was synthesized using random oligonucleotides and SuperScript II. Second strand cDNA was subsequently synthesized using DNA Polymerase I and RNase H. post adenylation of the 3 ends of DNA fragments Illumina PE adapter oligonucleotides were ligated and the library fragments were purified using the AMPure XP system Beckman Coulter. DNA fragments with ligated adaptors on both ends were selectively enriched using Illumina PCR Primer Cocktail in a 15 cycle PCR reaction. The products were purified AMPure XP system and quantified using the Agilent high sensitivity DNA assay on a Bioanalyzer 2100 system Agilent. The sequencing libraries were then sequenced at both ends for 150 bp using a Hiseq Xten platform Illumina by Shanghai Personal Biotechnology Cp. Ltd.     RNA-Seq TRANSCRIPTOMIC PCR PAIRED ILLUMINA HiSeq X Ten   SRP166817     24h2_clean_R2.fq.gz 24h2_clean_R1.fq.gz fastq fastq 6303822066.0 20873583.0 24h2 clean R1.fq.gz 0:151 1:151 A:1694366688;C:1441733171;G:1453717629;T:1711017538;N:2987040 151 151     1694366688 1441733171 1453717629 1711017538 2987040 SRX5767072 SRS4701366 SRA880742 Chinese Academy of Fishery Sciences|Yangtze River Fisheries Research Institute Chinese Academy of Fishery Sciences 2 0.9264 0.92591 0.13121 0.13204 0.7385 0.74227 0.50323 0.49357 151 151 B B biological fallback assumption illumina hiseq_era unknown random_priming trueseq bulk unknown unknown   China 2019-04-29 Undetermined Undetermined Cell Line Cell Line
49818 49818 SRR8983315 SRX5762615 SRS4697269 SRP166817 PRJNA498018 Danio rerio strain:ZFL cell line Genome sequencing PRJNA498018 Whole Genome Sequencing Transcriptional responses of ZFL exposed to microcystin LR       12h 5 sample exposed to microcystin for xxxh replicate #5   ecotype:not applicable|dev stage:not applicable|sex:not applicable|tissue:ZFL cell line|cell line:ZFL cell line|treatment:sample exposed to microcystin for xxxh replicate #5|BioSampleModel:Model organism or animal                 sample exposed to microcystin for xxxh replicate #5 12h 5 12h 5 Sequencing libraries were generated using the TruSeq RNA Sample Preparation Kit Illumina according to manufacturers instructions. Three micrograms of total RNA were used as initial material for library preparation. Briefly mRNA was purified using poly T oligo attached magnetic beads. Fragmentation of mRNA was conducted using divalent cations under elevated temperature in the Illumina proprietary fragmentation buffer. First strand cDNA was synthesized using random oligonucleotides and SuperScript II. Second strand cDNA was subsequently synthesized using DNA Polymerase I and RNase H. post adenylation of the 3 ends of DNA fragments Illumina PE adapter oligonucleotides were ligated and the library fragments were purified using the AMPure XP system Beckman Coulter. DNA fragments with ligated adaptors on both ends were selectively enriched using Illumina PCR Primer Cocktail in a 15 cycle PCR reaction. The products were purified AMPure XP system and quantified using the Agilent high sensitivity DNA assay on a Bioanalyzer 2100 system Agilent. The sequencing libraries were then sequenced at both ends for 150 bp using a Hiseq Xten platform Illumina by Shanghai Personal Biotechnology Cp. Ltd.     RNA-Seq TRANSCRIPTOMIC PCR PAIRED ILLUMINA HiSeq X Ten   SRP166817     12h2_clean_R1.fq.gz 12h2_clean_R2.fq.gz fastq fastq 5637564766.0 18667433.0 12h2 clean R1.fq.gz 0:151 1:151 A:1484457309;C:1319928521;G:1331480348;T:1498547286;N:3151302 151 151     1484457309 1319928521 1331480348 1498547286 3151302 SRX5762615 SRS4697269 SRA880316 Chinese Academy of Fishery Sciences|Yangtze River Fisheries Research Institute Chinese Academy of Fishery Sciences 2 0.93974 0.93913 0.09518 0.09588 0.74982 0.75331 0.49858 0.49712 151 151 B B biological fallback assumption illumina hiseq_era unknown random_priming trueseq bulk unknown unknown   China 2019-04-29 Undetermined Undetermined Cell Line Cell Line
49819 49819 SRR8983316 SRX5762614 SRS4697268 SRP166817 PRJNA498018 Danio rerio strain:ZFL cell line Genome sequencing PRJNA498018 Whole Genome Sequencing Transcriptional responses of ZFL exposed to microcystin LR       12h 4 sample exposed to microcystin for xxxh replicate #4   ecotype:not applicable|dev stage:not applicable|sex:not applicable|tissue:ZFL cell line|cell line:ZFL cell line|treatment:sample exposed to microcystin for xxxh replicate #4|BioSampleModel:Model organism or animal                 sample exposed to microcystin for xxxh replicate #4 12h 4 12h 4 Sequencing libraries were generated using the TruSeq RNA Sample Preparation Kit Illumina according to manufacturers instructions. Three micrograms of total RNA were used as initial material for library preparation. Briefly mRNA was purified using poly T oligo attached magnetic beads. Fragmentation of mRNA was conducted using divalent cations under elevated temperature in the Illumina proprietary fragmentation buffer. First strand cDNA was synthesized using random oligonucleotides and SuperScript II. Second strand cDNA was subsequently synthesized using DNA Polymerase I and RNase H. post adenylation of the 3 ends of DNA fragments Illumina PE adapter oligonucleotides were ligated and the library fragments were purified using the AMPure XP system Beckman Coulter. DNA fragments with ligated adaptors on both ends were selectively enriched using Illumina PCR Primer Cocktail in a 15 cycle PCR reaction. The products were purified AMPure XP system and quantified using the Agilent high sensitivity DNA assay on a Bioanalyzer 2100 system Agilent. The sequencing libraries were then sequenced at both ends for 150 bp using a Hiseq Xten platform Illumina by Shanghai Personal Biotechnology Cp. Ltd.     RNA-Seq TRANSCRIPTOMIC PCR PAIRED ILLUMINA HiSeq X Ten   SRP166817     12h1_clean_R2.fq.gz 12h1_clean_R1.fq.gz fastq fastq 5742037438.0 19013369.0 12h1 clean R1.fq.gz 0:151 1:151 A:1505967171;C:1350499061;G:1361100937;T:1521258216;N:3212053 151 151     1505967171 1350499061 1361100937 1521258216 3212053 SRX5762614 SRS4697268 SRA880316 Chinese Academy of Fishery Sciences|Yangtze River Fisheries Research Institute Chinese Academy of Fishery Sciences 2 0.9408 0.93974 0.09145 0.09155 0.75146 0.75501 0.49821 0.49267 151 151 B B biological fallback assumption illumina hiseq_era unknown random_priming trueseq bulk unknown unknown   China 2019-04-29 Undetermined Undetermined Cell Line Cell Line
49820 49820 SRR8983317 SRX5762613 SRS4697267 SRP166817 PRJNA498018 Danio rerio strain:ZFL cell line Genome sequencing PRJNA498018 Whole Genome Sequencing Transcriptional responses of ZFL exposed to microcystin LR       12h 6 sample exposed to microcystin for xxxh replicate #6   ecotype:not applicable|dev stage:not applicable|sex:not applicable|tissue:ZFL cell line|cell line:ZFL cell line|treatment:sample exposed to microcystin for xxxh replicate #6|BioSampleModel:Model organism or animal                 sample exposed to microcystin for xxxh replicate #6 12h 6 12h 6 Sequencing libraries were generated using the TruSeq RNA Sample Preparation Kit Illumina according to manufacturers instructions. Three micrograms of total RNA were used as initial material for library preparation. Briefly mRNA was purified using poly T oligo attached magnetic beads. Fragmentation of mRNA was conducted using divalent cations under elevated temperature in the Illumina proprietary fragmentation buffer. First strand cDNA was synthesized using random oligonucleotides and SuperScript II. Second strand cDNA was subsequently synthesized using DNA Polymerase I and RNase H. post adenylation of the 3 ends of DNA fragments Illumina PE adapter oligonucleotides were ligated and the library fragments were purified using the AMPure XP system Beckman Coulter. DNA fragments with ligated adaptors on both ends were selectively enriched using Illumina PCR Primer Cocktail in a 15 cycle PCR reaction. The products were purified AMPure XP system and quantified using the Agilent high sensitivity DNA assay on a Bioanalyzer 2100 system Agilent. The sequencing libraries were then sequenced at both ends for 150 bp using a Hiseq Xten platform Illumina by Shanghai Personal Biotechnology Cp. Ltd.     RNA-Seq TRANSCRIPTOMIC PCR PAIRED ILLUMINA HiSeq X Ten   SRP166817     12h3_clean_R2.fq.gz 12h3_clean_R1.fq.gz fastq fastq 5456135246.0 18066673.0 12h3 clean R1.fq.gz 0:151 1:151 A:1445780220;C:1268483624;G:1278423072;T:1460394475;N:3053855 151 151     1445780220 1268483624 1278423072 1460394475 3053855 SRX5762613 SRS4697267 SRA880316 Chinese Academy of Fishery Sciences|Yangtze River Fisheries Research Institute Chinese Academy of Fishery Sciences 2 0.93632 0.93624 0.10093 0.1015 0.7499 0.75367 0.50485 0.50164 151 151 B B biological fallback assumption illumina hiseq_era unknown random_priming trueseq bulk unknown unknown   China 2019-04-29 Undetermined Undetermined Cell Line Cell Line
49821 49821 SRR8982954 SRX5762254 SRS4696966 SRP166817 PRJNA498018 Danio rerio strain:ZFL cell line Genome sequencing PRJNA498018 Whole Genome Sequencing Transcriptional responses of ZFL exposed to microcystin LR       6h 6 sample exposed to microcystin for xxxh replicate #6   ecotype:not applicable|dev stage:not applicable|sex:not applicable|tissue:ZFL cell line|cell line:ZFL cell line|treatment:sample exposed to microcystin for xxxh replicate #6|BioSampleModel:Model organism or animal                 sample exposed to microcystin for xxxh replicate #6 6h 6 6h 6 Sequencing libraries were generated using the TruSeq RNA Sample Preparation Kit Illumina according to manufacturers instructions. Three micrograms of total RNA were used as initial material for library preparation. Briefly mRNA was purified using poly T oligo attached magnetic beads. Fragmentation of mRNA was conducted using divalent cations under elevated temperature in the Illumina proprietary fragmentation buffer. First strand cDNA was synthesized using random oligonucleotides and SuperScript II. Second strand cDNA was subsequently synthesized using DNA Polymerase I and RNase H. post adenylation of the 3 ends of DNA fragments Illumina PE adapter oligonucleotides were ligated and the library fragments were purified using the AMPure XP system Beckman Coulter. DNA fragments with ligated adaptors on both ends were selectively enriched using Illumina PCR Primer Cocktail in a 15 cycle PCR reaction. The products were purified AMPure XP system and quantified using the Agilent high sensitivity DNA assay on a Bioanalyzer 2100 system Agilent. The sequencing libraries were then sequenced at both ends for 150 bp using a Hiseq Xten platform Illumina by Shanghai Personal Biotechnology Cp. Ltd.     RNA-Seq TRANSCRIPTOMIC PCR PAIRED ILLUMINA HiSeq X Ten   SRP166817     6h3_clean_R1.fq.gz 6h3_clean_R2.fq.gz fastq fastq 7939056332.0 26288266.0 6h3 clean R1.fq.gz 0:151 1:151 A:2043932346;C:1908038526;G:1925388013;T:2058202469;N:3494978 151 151     2043932346 1908038526 1925388013 2058202469 3494978 SRX5762254 SRS4696966 SRA880287 Chinese Academy of Fishery Sciences|Yangtze River Fisheries Research Institute Chinese Academy of Fishery Sciences 2 0.94661 0.94661 0.07559 0.07638 0.76134 0.76394 0.49736 0.50336 151 151 B B biological fallback assumption illumina hiseq_era unknown random_priming trueseq bulk unknown unknown   China 2019-04-29 Undetermined Undetermined Cell Line Cell Line
49822 49822 SRR8982955 SRX5762253 SRS4696965 SRP166817 PRJNA498018 Danio rerio strain:ZFL cell line Genome sequencing PRJNA498018 Whole Genome Sequencing Transcriptional responses of ZFL exposed to microcystin LR       6h 5 sample exposed to microcystin for xxxh replicate #5   ecotype:not applicable|dev stage:not applicable|sex:not applicable|tissue:ZFL cell line|cell line:ZFL cell line|treatment:sample exposed to microcystin for xxxh replicate #5|BioSampleModel:Model organism or animal                 sample exposed to microcystin for xxxh replicate #5 6h 5 6h 5 Sequencing libraries were generated using the TruSeq RNA Sample Preparation Kit Illumina according to manufacturers instructions. Three micrograms of total RNA were used as initial material for library preparation. Briefly mRNA was purified using poly T oligo attached magnetic beads. Fragmentation of mRNA was conducted using divalent cations under elevated temperature in the Illumina proprietary fragmentation buffer. First strand cDNA was synthesized using random oligonucleotides and SuperScript II. Second strand cDNA was subsequently synthesized using DNA Polymerase I and RNase H. post adenylation of the 3 ends of DNA fragments Illumina PE adapter oligonucleotides were ligated and the library fragments were purified using the AMPure XP system Beckman Coulter. DNA fragments with ligated adaptors on both ends were selectively enriched using Illumina PCR Primer Cocktail in a 15 cycle PCR reaction. The products were purified AMPure XP system and quantified using the Agilent high sensitivity DNA assay on a Bioanalyzer 2100 system Agilent. The sequencing libraries were then sequenced at both ends for 150 bp using a Hiseq Xten platform Illumina by Shanghai Personal Biotechnology Cp. Ltd.     RNA-Seq TRANSCRIPTOMIC PCR PAIRED ILLUMINA HiSeq X Ten   SRP166817     6h2_clean_R1.fq.gz 6h2_clean_R2.fq.gz fastq fastq 5431283968.0 17984384.0 6h2 clean R1.fq.gz 0:151 1:151 A:1432960105;C:1269588003;G:1284040697;T:1441657813;N:3037350 151 151     1432960105 1269588003 1284040697 1441657813 3037350 SRX5762253 SRS4696965 SRA880287 Chinese Academy of Fishery Sciences|Yangtze River Fisheries Research Institute Chinese Academy of Fishery Sciences 2 0.9325 0.93223 0.11537 0.11461 0.7433 0.7457 0.49688 0.50558 151 151 B B biological fallback assumption illumina hiseq_era unknown random_priming trueseq bulk unknown unknown   China 2019-04-29 Undetermined Undetermined Cell Line Cell Line
49823 49823 SRR8982956 SRX5762252 SRS4696964 SRP166817 PRJNA498018 Danio rerio strain:ZFL cell line Genome sequencing PRJNA498018 Whole Genome Sequencing Transcriptional responses of ZFL exposed to microcystin LR       6h 4 sample exposed to microcystin for xxxh replicate #4   ecotype:not applicable|dev stage:not applicable|sex:not applicable|tissue:ZFL cell line|cell line:ZFL cell line|treatment:sample exposed to microcystin for xxxh replicate #4|BioSampleModel:Model organism or animal                 sample exposed to microcystin for xxxh replicate #4 6h 4 6h 4 Sequencing libraries were generated using the TruSeq RNA Sample Preparation Kit Illumina according to manufacturers instructions. Three micrograms of total RNA were used as initial material for library preparation. Briefly mRNA was purified using poly T oligo attached magnetic beads. Fragmentation of mRNA was conducted using divalent cations under elevated temperature in the Illumina proprietary fragmentation buffer. First strand cDNA was synthesized using random oligonucleotides and SuperScript II. Second strand cDNA was subsequently synthesized using DNA Polymerase I and RNase H. post adenylation of the 3 ends of DNA fragments Illumina PE adapter oligonucleotides were ligated and the library fragments were purified using the AMPure XP system Beckman Coulter. DNA fragments with ligated adaptors on both ends were selectively enriched using Illumina PCR Primer Cocktail in a 15 cycle PCR reaction. The products were purified AMPure XP system and quantified using the Agilent high sensitivity DNA assay on a Bioanalyzer 2100 system Agilent. The sequencing libraries were then sequenced at both ends for 150 bp using a Hiseq Xten platform Illumina by Shanghai Personal Biotechnology Cp. Ltd.     RNA-Seq TRANSCRIPTOMIC PCR PAIRED ILLUMINA HiSeq X Ten   SRP166817     6h1_clean_R1.fq.gz 6h1_clean_R2.fq.gz fastq fastq 5561923128.0 18416964.0 6h1 clean R1.fq.gz 0:151 1:151 A:1439698401;C:1327963901;G:1340151064;T:1450995064;N:3114698 151 151     1439698401 1327963901 1340151064 1450995064 3114698 SRX5762252 SRS4696964 SRA880287 Chinese Academy of Fishery Sciences|Yangtze River Fisheries Research Institute Chinese Academy of Fishery Sciences 2 0.9438 0.94314 0.08661 0.08624 0.75613 0.75858 0.49468 0.47945 151 151 B B biological fallback assumption illumina hiseq_era unknown random_priming trueseq bulk unknown unknown   China 2019-04-29 Undetermined Undetermined Cell Line Cell Line
49824 49824 SRR8981191 SRX5760491 SRS4695477 SRP166817 PRJNA498018 Danio rerio strain:ZFL cell line Genome sequencing PRJNA498018 Whole Genome Sequencing Transcriptional responses of ZFL exposed to microcystin LR       3h 5 sample exposed to microcystin for xxxh replicate #5   ecotype:not applicable|dev stage:not applicable|sex:not applicable|tissue:ZFL cell line|cell line:ZFL cell line|treatment:sample exposed to microcystin for xxxh replicate #5|BioSampleModel:Model organism or animal                 sample exposed to microcystin for xxxh replicate #5 3h 5 3h 5 Sequencing libraries were generated using the TruSeq RNA Sample Preparation Kit Illumina according to manufacturers instructions. Three micrograms of total RNA were used as initial material for library preparation. Briefly mRNA was purified using poly T oligo attached magnetic beads. Fragmentation of mRNA was conducted using divalent cations under elevated temperature in the Illumina proprietary fragmentation buffer. First strand cDNA was synthesized using random oligonucleotides and SuperScript II. Second strand cDNA was subsequently synthesized using DNA Polymerase I and RNase H. post adenylation of the 3 ends of DNA fragments Illumina PE adapter oligonucleotides were ligated and the library fragments were purified using the AMPure XP system Beckman Coulter. DNA fragments with ligated adaptors on both ends were selectively enriched using Illumina PCR Primer Cocktail in a 15 cycle PCR reaction. The products were purified AMPure XP system and quantified using the Agilent high sensitivity DNA assay on a Bioanalyzer 2100 system Agilent. The sequencing libraries were then sequenced at both ends for 150 bp using a Hiseq Xten platform Illumina by Shanghai Personal Biotechnology Cp. Ltd.     RNA-Seq TRANSCRIPTOMIC PCR PAIRED ILLUMINA HiSeq X Ten   SRP166817     3h2_clean_R1.fq.gz 3h2_clean_R2.fq.gz fastq fastq 5426694474.0 17969187.0 3h2 clean R1.fq.gz 0:151 1:151 A:1455679038;C:1243463586;G:1253806438;T:1470703342;N:3042070 151 151     1455679038 1243463586 1253806438 1470703342 3042070 SRX5760491 SRS4695477 SRA880231 Chinese Academy of Fishery Sciences|Yangtze River Fisheries Research Institute Chinese Academy of Fishery Sciences 2 0.92775 0.9265 0.13003 0.13039 0.74257 0.74679 0.48728 0.50236 151 151 B B biological fallback assumption illumina hiseq_era unknown random_priming trueseq bulk unknown unknown   China 2019-04-28 Undetermined Undetermined Cell Line Cell Line
49825 49825 SRR8981192 SRX5760490 SRS4695476 SRP166817 PRJNA498018 Danio rerio strain:ZFL cell line Genome sequencing PRJNA498018 Whole Genome Sequencing Transcriptional responses of ZFL exposed to microcystin LR       3h 4 sample exposed to microcystin for xxxh replicate #4   ecotype:not applicable|dev stage:not applicable|sex:not applicable|tissue:ZFL cell line|cell line:ZFL cell line|treatment:sample exposed to microcystin for xxxh replicate #4|BioSampleModel:Model organism or animal                 sample exposed to microcystin for xxxh replicate #4 3h 4 3h 4 Sequencing libraries were generated using the TruSeq RNA Sample Preparation Kit Illumina according to manufacturers instructions. Three micrograms of total RNA were used as initial material for library preparation. Briefly mRNA was purified using poly T oligo attached magnetic beads. Fragmentation of mRNA was conducted using divalent cations under elevated temperature in the Illumina proprietary fragmentation buffer. First strand cDNA was synthesized using random oligonucleotides and SuperScript II. Second strand cDNA was subsequently synthesized using DNA Polymerase I and RNase H. post adenylation of the 3 ends of DNA fragments Illumina PE adapter oligonucleotides were ligated and the library fragments were purified using the AMPure XP system Beckman Coulter. DNA fragments with ligated adaptors on both ends were selectively enriched using Illumina PCR Primer Cocktail in a 15 cycle PCR reaction. The products were purified AMPure XP system and quantified using the Agilent high sensitivity DNA assay on a Bioanalyzer 2100 system Agilent. The sequencing libraries were then sequenced at both ends for 150 bp using a Hiseq Xten platform Illumina by Shanghai Personal Biotechnology Cp. Ltd.     RNA-Seq TRANSCRIPTOMIC PCR PAIRED ILLUMINA HiSeq X Ten   SRP166817     3h1_clean_R1.fq.gz 3h1_clean_R2.fq.gz fastq fastq 6381408584.0 21130492.0 3h1 clean R1.fq.gz 0:151 1:151 A:1704493392;C:1471437290;G:1482728107;T:1720014953;N:2734842 151 151     1704493392 1471437290 1482728107 1720014953 2734842 SRX5760490 SRS4695476 SRA880231 Chinese Academy of Fishery Sciences|Yangtze River Fisheries Research Institute Chinese Academy of Fishery Sciences 2 0.93 0.93014 0.11977 0.12069 0.74434 0.74864 0.49627 0.50428 151 151 B B biological fallback assumption illumina hiseq_era unknown random_priming trueseq bulk unknown unknown   China 2019-04-28 Undetermined Undetermined Cell Line Cell Line
49826 49826 SRR8981193 SRX5760489 SRS4695475 SRP166817 PRJNA498018 Danio rerio strain:ZFL cell line Genome sequencing PRJNA498018 Whole Genome Sequencing Transcriptional responses of ZFL exposed to microcystin LR       3h 6 sample exposed to microcystin for xxxh replicate #6   ecotype:not applicable|dev stage:not applicable|sex:not applicable|tissue:ZFL cell line|cell line:ZFL cell line|treatment:sample exposed to microcystin for xxxh replicate #6|BioSampleModel:Model organism or animal                 sample exposed to microcystin for xxxh replicate #6 3h 6 3h 6 Sequencing libraries were generated using the TruSeq RNA Sample Preparation Kit Illumina according to manufacturers instructions. Three micrograms of total RNA were used as initial material for library preparation. Briefly mRNA was purified using poly T oligo attached magnetic beads. Fragmentation of mRNA was conducted using divalent cations under elevated temperature in the Illumina proprietary fragmentation buffer. First strand cDNA was synthesized using random oligonucleotides and SuperScript II. Second strand cDNA was subsequently synthesized using DNA Polymerase I and RNase H. post adenylation of the 3 ends of DNA fragments Illumina PE adapter oligonucleotides were ligated and the library fragments were purified using the AMPure XP system Beckman Coulter. DNA fragments with ligated adaptors on both ends were selectively enriched using Illumina PCR Primer Cocktail in a 15 cycle PCR reaction. The products were purified AMPure XP system and quantified using the Agilent high sensitivity DNA assay on a Bioanalyzer 2100 system Agilent. The sequencing libraries were then sequenced at both ends for 150 bp using a Hiseq Xten platform Illumina by Shanghai Personal Biotechnology Cp. Ltd.     RNA-Seq TRANSCRIPTOMIC PCR PAIRED ILLUMINA HiSeq X Ten   SRP166817     3h3_clean_R1.fq.gz 3h3_clean_R2.fq.gz fastq fastq 6058080740.0 20059870.0 3h3 clean R1.fq.gz 0:151 1:151 A:1623401423;C:1391994584;G:1400166489;T:1639132542;N:3385702 151 151     1623401423 1391994584 1400166489 1639132542 3385702 SRX5760489 SRS4695475 SRA880231 Chinese Academy of Fishery Sciences|Yangtze River Fisheries Research Institute Chinese Academy of Fishery Sciences 2 0.93298 0.93201 0.10907 0.10885 0.75059 0.75463 0.50656 0.49273 151 151 B B biological fallback assumption illumina hiseq_era unknown random_priming trueseq bulk unknown unknown   China 2019-04-28 Undetermined Undetermined Cell Line Cell Line
49827 49827 SRR8961086 SRX5740640 SRS4676161 SRP166817 PRJNA498018 Danio rerio strain:ZFL cell line Genome sequencing PRJNA498018 Whole Genome Sequencing Transcriptional responses of ZFL exposed to microcystin LR       1h 6 sample exposed to microcystin for xxxh replicate #6   ecotype:not applicable|dev stage:not applicable|sex:not applicable|tissue:ZFL cell line|cell line:ZFL cell line|treatment:sample exposed to microcystin for xxxh replicate #6|BioSampleModel:Model organism or animal                 sample exposed to microcystin for xxxh replicate #6 1h 6 1h 6 Sequencing libraries were generated using the TruSeq RNA Sample Preparation Kit Illumina according to manufacturers instructions. Three micrograms of total RNA were used as initial material for library preparation. Briefly mRNA was purified using poly T oligo attached magnetic beads. Fragmentation of mRNA was conducted using divalent cations under elevated temperature in the Illumina proprietary fragmentation buffer. First strand cDNA was synthesized using random oligonucleotides and SuperScript II. Second strand cDNA was subsequently synthesized using DNA Polymerase I and RNase H. post adenylation of the 3 ends of DNA fragments Illumina PE adapter oligonucleotides were ligated and the library fragments were purified using the AMPure XP system Beckman Coulter. DNA fragments with ligated adaptors on both ends were selectively enriched using Illumina PCR Primer Cocktail in a 15 cycle PCR reaction. The products were purified AMPure XP system and quantified using the Agilent high sensitivity DNA assay on a Bioanalyzer 2100 system Agilent. The sequencing libraries were then sequenced at both ends for 150 bp using a Hiseq Xten platform Illumina by Shanghai Personal Biotechnology Cp. Ltd.     RNA-Seq TRANSCRIPTOMIC PCR PAIRED ILLUMINA HiSeq X Ten   SRP166817     1h3_clean_R1.fq.gz 1h3_clean_R2.fq.gz fastq fastq 6472187368.0 21431084.0 1h3 clean R1.fq.gz 0:151 1:151 A:1780621052;C:1438221849;G:1453983421;T:1796459482;N:2901564 151 151     1780621052 1438221849 1453983421 1796459482 2901564 SRX5740640 SRS4676161 SRA879791 Chinese Academy of Fishery Sciences|Yangtze River Fisheries Research Institute Chinese Academy of Fishery Sciences 2 0.92158 0.91814 0.14503 0.14358 0.75775 0.75982 0.50094 0.49955 151 151 B B biological fallback assumption illumina hiseq_era unknown random_priming trueseq bulk unknown unknown   China 2019-04-26 Undetermined Undetermined Cell Line Cell Line
49828 49828 SRR8961087 SRX5740639 SRS4676160 SRP166817 PRJNA498018 Danio rerio strain:ZFL cell line Genome sequencing PRJNA498018 Whole Genome Sequencing Transcriptional responses of ZFL exposed to microcystin LR       1h 4 sample exposed to microcystin for xxxh replicate #4   ecotype:not applicable|dev stage:not applicable|sex:not applicable|tissue:ZFL cell line|cell line:ZFL cell line|treatment:sample exposed to microcystin for xxxh replicate #4|BioSampleModel:Model organism or animal                 sample exposed to microcystin for xxxh replicate #4 1h 4 1h 4 Sequencing libraries were generated using the TruSeq RNA Sample Preparation Kit Illumina according to manufacturers instructions. Three micrograms of total RNA were used as initial material for library preparation. Briefly mRNA was purified using poly T oligo attached magnetic beads. Fragmentation of mRNA was conducted using divalent cations under elevated temperature in the Illumina proprietary fragmentation buffer. First strand cDNA was synthesized using random oligonucleotides and SuperScript II. Second strand cDNA was subsequently synthesized using DNA Polymerase I and RNase H. post adenylation of the 3 ends of DNA fragments Illumina PE adapter oligonucleotides were ligated and the library fragments were purified using the AMPure XP system Beckman Coulter. DNA fragments with ligated adaptors on both ends were selectively enriched using Illumina PCR Primer Cocktail in a 15 cycle PCR reaction. The products were purified AMPure XP system and quantified using the Agilent high sensitivity DNA assay on a Bioanalyzer 2100 system Agilent. The sequencing libraries were then sequenced at both ends for 150 bp using a Hiseq Xten platform Illumina by Shanghai Personal Biotechnology Cp. Ltd.     RNA-Seq TRANSCRIPTOMIC PCR PAIRED ILLUMINA HiSeq X Ten   SRP166817     1h1_clean_R1.fq.gz 1h1_clean_R2.fq.gz fastq fastq 6177620796.0 20455698.0 1h1 clean R1.fq.gz 0:151 1:151 A:1646973061;C:1422549043;G:1441385824;T:1663912518;N:2800350 151 151     1646973061 1422549043 1441385824 1663912518 2800350 SRX5740639 SRS4676160 SRA879791 Chinese Academy of Fishery Sciences|Yangtze River Fisheries Research Institute Chinese Academy of Fishery Sciences 2 0.93085 0.92866 0.12633 0.12579 0.75812 0.76136 0.48992 0.50959 151 151 B B biological fallback assumption illumina hiseq_era unknown random_priming trueseq bulk unknown unknown   China 2019-04-26 Undetermined Undetermined Cell Line Cell Line
49829 49829 SRR8961088 SRX5740638 SRS4676159 SRP166817 PRJNA498018 Danio rerio strain:ZFL cell line Genome sequencing PRJNA498018 Whole Genome Sequencing Transcriptional responses of ZFL exposed to microcystin LR       1h 5 sample exposed to microcystin for xxxh replicate #5   ecotype:not applicable|dev stage:not applicable|sex:not applicable|tissue:ZFL cell line|cell line:ZFL cell line|treatment:sample exposed to microcystin for xxxh replicate #5|BioSampleModel:Model organism or animal                 sample exposed to microcystin for xxxh replicate #5 1h 5 1h 5 Sequencing libraries were generated using the TruSeq RNA Sample Preparation Kit Illumina according to manufacturers instructions. Three micrograms of total RNA were used as initial material for library preparation. Briefly mRNA was purified using poly T oligo attached magnetic beads. Fragmentation of mRNA was conducted using divalent cations under elevated temperature in the Illumina proprietary fragmentation buffer. First strand cDNA was synthesized using random oligonucleotides and SuperScript II. Second strand cDNA was subsequently synthesized using DNA Polymerase I and RNase H. post adenylation of the 3 ends of DNA fragments Illumina PE adapter oligonucleotides were ligated and the library fragments were purified using the AMPure XP system Beckman Coulter. DNA fragments with ligated adaptors on both ends were selectively enriched using Illumina PCR Primer Cocktail in a 15 cycle PCR reaction. The products were purified AMPure XP system and quantified using the Agilent high sensitivity DNA assay on a Bioanalyzer 2100 system Agilent. The sequencing libraries were then sequenced at both ends for 150 bp using a Hiseq Xten platform Illumina by Shanghai Personal Biotechnology Cp. Ltd.     RNA-Seq TRANSCRIPTOMIC PCR PAIRED ILLUMINA HiSeq X Ten   SRP166817     1h2_clean_R2.fq.gz 1h2_clean_R1.fq.gz fastq fastq 5616651266.0 18598183.0 1h2 clean R1.fq.gz 0:151 1:151 A:1492544948;C:1296049637;G:1317613246;T:1507292547;N:3150888 151 151     1492544948 1296049637 1317613246 1507292547 3150888 SRX5740638 SRS4676159 SRA879791 Chinese Academy of Fishery Sciences|Yangtze River Fisheries Research Institute Chinese Academy of Fishery Sciences 2 0.93168 0.93082 0.12771 0.12751 0.75653 0.75964 0.508 0.51051 151 151 B B biological fallback assumption illumina hiseq_era unknown random_priming trueseq bulk unknown unknown   China 2019-04-26 Undetermined Undetermined Cell Line Cell Line
49830 49830 SRR8959882 SRX5739436 SRS4675912 SRP166817 PRJNA498018 Danio rerio strain:ZFL cell line Genome sequencing PRJNA498018 Whole Genome Sequencing Transcriptional responses of ZFL exposed to microcystin LR       control replicate #6 ck 6   replicate:replicate=#6|ecotype:not applicable|dev stage:not applicable|sex:not applicable|tissue:ZFL cell line|BioSampleModel:Model organism or animal                 replicate 6 ck 6 ck 6 Sequencing libraries were generated using the TruSeq RNA Sample Preparation Kit Illumina according to manufacturers instructions. Three micrograms of total RNA were used as initial material for library preparation. Briefly mRNA was purified using poly T oligo attached magnetic beads. Fragmentation of mRNA was conducted using divalent cations under elevated temperature in the Illumina proprietary fragmentation buffer. First strand cDNA was synthesized using random oligonucleotides and SuperScript II. Second strand cDNA was subsequently synthesized using DNA Polymerase I and RNase H. post adenylation of the 3 ends of DNA fragments Illumina PE adapter oligonucleotides were ligated and the library fragments were purified using the AMPure XP system Beckman Coulter. DNA fragments with ligated adaptors on both ends were selectively enriched using Illumina PCR Primer Cocktail in a 15 cycle PCR reaction. The products were purified AMPure XP system and quantified using the Agilent high sensitivity DNA assay on a Bioanalyzer 2100 system Agilent. The sequencing libraries were then sequenced at both ends for 150 bp using a Hiseq Xten platform Illumina by Shanghai Personal Biotechnology Cp. Ltd.     RNA-Seq TRANSCRIPTOMIC PCR PAIRED ILLUMINA HiSeq X Ten   SRP166817     CK3_clean_R1.fq.gz CK3_clean_R2.fq.gz fastq fastq 6417346282.0 21249491.0 CK3 clean R1.fq.gz 0:151 1:151 A:1753073270;C:1445910840;G:1452693305;T:1762084157;N:3584710 151 151     1753073270 1445910840 1452693305 1762084157 3584710 SRX5739436 SRS4675912 SRA879767 Chinese Academy of Fishery Sciences|Yangtze River Fisheries Research Institute Chinese Academy of Fishery Sciences 2 0.92653 0.92662 0.11696 0.11682 0.75166 0.75379 0.50678 0.50594 151 151 B B biological fallback assumption illumina hiseq_era unknown random_priming trueseq bulk unknown unknown   China 2019-04-26 Undetermined Undetermined Cell Line Cell Line
49831 49831 SRR8959883 SRX5739435 SRS4675911 SRP166817 PRJNA498018 Danio rerio strain:ZFL cell line Genome sequencing PRJNA498018 Whole Genome Sequencing Transcriptional responses of ZFL exposed to microcystin LR       control replicate #4 ck 4   replicate:replicate=#4|ecotype:not applicable|dev stage:not applicable|sex:not applicable|tissue:ZFL cell line|BioSampleModel:Model organism or animal                 replicate 4 ck 4 ck 4 Sequencing libraries were generated using the TruSeq RNA Sample Preparation Kit Illumina according to manufacturers instructions. Three micrograms of total RNA were used as initial material for library preparation. Briefly mRNA was purified using poly T oligo attached magnetic beads. Fragmentation of mRNA was conducted using divalent cations under elevated temperature in the Illumina proprietary fragmentation buffer. First strand cDNA was synthesized using random oligonucleotides and SuperScript II. Second strand cDNA was subsequently synthesized using DNA Polymerase I and RNase H. post adenylation of the 3 ends of DNA fragments Illumina PE adapter oligonucleotides were ligated and the library fragments were purified using the AMPure XP system Beckman Coulter. DNA fragments with ligated adaptors on both ends were selectively enriched using Illumina PCR Primer Cocktail in a 15 cycle PCR reaction. The products were purified AMPure XP system and quantified using the Agilent high sensitivity DNA assay on a Bioanalyzer 2100 system Agilent. The sequencing libraries were then sequenced at both ends for 150 bp using a Hiseq Xten platform Illumina by Shanghai Personal Biotechnology Cp. Ltd.     RNA-Seq TRANSCRIPTOMIC PCR PAIRED ILLUMINA HiSeq X Ten   SRP166817     CK1_clean_R1.fq.gz CK1_clean_R2.fq.gz fastq fastq 5659831830.0 18741165.0 CK1 clean R1.fq.gz 0:151 1:151 A:1532952307;C:1289729700;G:1295220307;T:1538761250;N:3168266 151 151     1532952307 1289729700 1295220307 1538761250 3168266 SRX5739435 SRS4675911 SRA879767 Chinese Academy of Fishery Sciences|Yangtze River Fisheries Research Institute Chinese Academy of Fishery Sciences 2 0.92518 0.92535 0.11332 0.11378 0.74919 0.75235 0.50223 0.49803 151 151 B B biological fallback assumption illumina hiseq_era unknown random_priming trueseq bulk unknown unknown   China 2019-04-26 Undetermined Undetermined Cell Line Cell Line
49832 49832 SRR8959884 SRX5739434 SRS4675910 SRP166817 PRJNA498018 Danio rerio strain:ZFL cell line Genome sequencing PRJNA498018 Whole Genome Sequencing Transcriptional responses of ZFL exposed to microcystin LR       control replicate #5 ck 5   replicate:replicate=#5|ecotype:not applicable|dev stage:not applicable|sex:not applicable|tissue:ZFL cell line|BioSampleModel:Model organism or animal                 replicate 5 ck 5 ck 5 Sequencing libraries were generated using the TruSeq RNA Sample Preparation Kit Illumina according to manufacturers instructions. Three micrograms of total RNA were used as initial material for library preparation. Briefly mRNA was purified using poly T oligo attached magnetic beads. Fragmentation of mRNA was conducted using divalent cations under elevated temperature in the Illumina proprietary fragmentation buffer. First strand cDNA was synthesized using random oligonucleotides and SuperScript II. Second strand cDNA was subsequently synthesized using DNA Polymerase I and RNase H. post adenylation of the 3 ends of DNA fragments Illumina PE adapter oligonucleotides were ligated and the library fragments were purified using the AMPure XP system Beckman Coulter. DNA fragments with ligated adaptors on both ends were selectively enriched using Illumina PCR Primer Cocktail in a 15 cycle PCR reaction. The products were purified AMPure XP system and quantified using the Agilent high sensitivity DNA assay on a Bioanalyzer 2100 system Agilent. The sequencing libraries were then sequenced at both ends for 150 bp using a Hiseq Xten platform Illumina by Shanghai Personal Biotechnology Cp. Ltd.     RNA-Seq TRANSCRIPTOMIC PCR PAIRED ILLUMINA HiSeq X Ten   SRP166817     CK2_clean_R1.fq.gz CK2_clean_R2.fq.gz fastq fastq 5880727616.0 19472608.0 CK2 clean R1.fq.gz 0:151 1:151 A:1575999691;C:1352057289;G:1366253556;T:1583120335;N:3296745 151 151     1575999691 1352057289 1366253556 1583120335 3296745 SRX5739434 SRS4675910 SRA879767 Chinese Academy of Fishery Sciences|Yangtze River Fisheries Research Institute Chinese Academy of Fishery Sciences 2 0.93653 0.9349 0.09037 0.09026 0.76262 0.76572 0.51143 0.50376 151 151 B B biological fallback assumption illumina hiseq_era unknown random_priming trueseq bulk unknown unknown   China 2019-04-26 Undetermined Undetermined Cell Line Cell Line
49833 49833 SRR8133154 SRX4954244 SRS3995636 SRP166817 PRJNA498018 Danio rerio strain:ZFL cell line Genome sequencing PRJNA498018 Whole Genome Sequencing Transcriptional responses of ZFL exposed to microcystin LR       sample exposed to microcystin for xxx hour replicate #1 24h 1   isolate:ZFL cell line|dev stage:not applicable|sex:not applicable|tissue:cultured cell line|cell line:ZFL|cell type:epithelia|treatment:exposed to microcystin for xxx hour replicate #1|BioSampleModel:Model organism or animal                 sample exposed to microcystin for xxx hour replicate #1 24h 1 24h 1 Sequencing libraries were generated using the TruSeq RNA Sample Preparation Kit Illumina according to manufacturers instructions. Three micrograms of total RNA were used as initial material for library preparation. Briefly mRNA was purified using poly T oligo attached magnetic beads. Fragmentation of mRNA was conducted using divalent cations under elevated temperature in the Illumina proprietary fragmentation buffer. First strand cDNA was synthesized using random oligonucleotides and SuperScript II. Second strand cDNA was subsequently synthesized using DNA Polymerase I and RNase H. post adenylation of the 3 ends of DNA fragments Illumina PE adapter oligonucleotides were ligated and the library fragments were purified using the AMPure XP system Beckman Coulter. DNA fragments with ligated adaptors on both ends were selectively enriched using Illumina PCR Primer Cocktail in a 15 cycle PCR reaction. The products were purified AMPure XP system and quantified using the Agilent high sensitivity DNA assay on a Bioanalyzer 2100 system Agilent. The sequencing libraries were then sequenced at both ends for 150 bp using a Hiseq Xten platform Illumina by Shanghai Personal Biotechnology Cp. Ltd.     RNA-Seq TRANSCRIPTOMIC PCR PAIRED ILLUMINA HiSeq X Ten   SRP166817     24h-1_R1.fastq.gz 24h-1_R2.fastq.gz fastq fastq 6408674352.0 21220776.0 24h 1 R1.fastq.gz 0:151 1:151 A:1752104547;C:1447174976;G:1451968217;T:1757298923;N:127689 151 151     1752104547 1447174976 1451968217 1757298923 127689 SRX4954244 SRS3995636 SRA800477 Chinese Academy of Fishery Sciences|Yangtze River Fisheries Research Institute Chinese Academy of Fishery Sciences 2 0.9212 0.91969 0.14419 0.14446 0.73677 0.74363 0.49228 0.49725 151 151 B B biological fallback assumption illumina hiseq_era unknown random_priming trueseq bulk unknown unknown   China 2018-10-30 Undetermined Undetermined Cell Line Cell Line
49834 49834 SRR8133155 SRX4954243 SRS3995635 SRP166817 PRJNA498018 Danio rerio strain:ZFL cell line Genome sequencing PRJNA498018 Whole Genome Sequencing Transcriptional responses of ZFL exposed to microcystin LR       sample exposed to microcystin for xxx hour replicate #2 24h 2   isolate:ZFL cell line|dev stage:not applicable|sex:not applicable|tissue:cultured cell line|cell line:ZFL|cell type:epithelia|treatment:exposed to microcystin for xxx hour replicate #2|BioSampleModel:Model organism or animal                 sample exposed to microcystin for xxx hour replicate #2 24h 2 24h 2 Sequencing libraries were generated using the TruSeq RNA Sample Preparation Kit Illumina according to manufacturers instructions. Three micrograms of total RNA were used as initial material for library preparation. Briefly mRNA was purified using poly T oligo attached magnetic beads. Fragmentation of mRNA was conducted using divalent cations under elevated temperature in the Illumina proprietary fragmentation buffer. First strand cDNA was synthesized using random oligonucleotides and SuperScript II. Second strand cDNA was subsequently synthesized using DNA Polymerase I and RNase H. post adenylation of the 3 ends of DNA fragments Illumina PE adapter oligonucleotides were ligated and the library fragments were purified using the AMPure XP system Beckman Coulter. DNA fragments with ligated adaptors on both ends were selectively enriched using Illumina PCR Primer Cocktail in a 15 cycle PCR reaction. The products were purified AMPure XP system and quantified using the Agilent high sensitivity DNA assay on a Bioanalyzer 2100 system Agilent. The sequencing libraries were then sequenced at both ends for 150 bp using a Hiseq Xten platform Illumina by Shanghai Personal Biotechnology Cp. Ltd.     RNA-Seq TRANSCRIPTOMIC PCR PAIRED ILLUMINA HiSeq X Ten   SRP166817     24h-2_R1.fastq.gz 24h-2_R2.fastq.gz fastq fastq 6331528754.0 20965327.0 24h 2 R1.fastq.gz 0:151 1:151 A:1725083256;C:1436372645;G:1442486884;T:1727457912;N:128057 151 151     1725083256 1436372645 1442486884 1727457912 128057 SRX4954243 SRS3995635 SRA800477 Chinese Academy of Fishery Sciences|Yangtze River Fisheries Research Institute Chinese Academy of Fishery Sciences 2 0.92281 0.92303 0.13905 0.14045 0.73708 0.74401 0.50843 0.50295 151 151 B B biological fallback assumption illumina hiseq_era unknown random_priming trueseq bulk unknown unknown   China 2019-01-01 Undetermined Undetermined Cell Line Cell Line
49835 49835 SRR8133156 SRX4954242 SRS3995634 SRP166817 PRJNA498018 Danio rerio strain:ZFL cell line Genome sequencing PRJNA498018 Whole Genome Sequencing Transcriptional responses of ZFL exposed to microcystin LR       sample exposed to microcystin for xxx hour replicate #3 24h 3   isolate:ZFL cell line|dev stage:not applicable|sex:not applicable|tissue:cultured cell line|cell line:ZFL|cell type:epithelia|treatment:exposed to microcystin for xxx hour replicate #3|BioSampleModel:Model organism or animal                 sample exposed to microcystin for xxx hour replicate #3 24h 3 24h 3 Sequencing libraries were generated using the TruSeq RNA Sample Preparation Kit Illumina according to manufacturers instructions. Three micrograms of total RNA were used as initial material for library preparation. Briefly mRNA was purified using poly T oligo attached magnetic beads. Fragmentation of mRNA was conducted using divalent cations under elevated temperature in the Illumina proprietary fragmentation buffer. First strand cDNA was synthesized using random oligonucleotides and SuperScript II. Second strand cDNA was subsequently synthesized using DNA Polymerase I and RNase H. post adenylation of the 3 ends of DNA fragments Illumina PE adapter oligonucleotides were ligated and the library fragments were purified using the AMPure XP system Beckman Coulter. DNA fragments with ligated adaptors on both ends were selectively enriched using Illumina PCR Primer Cocktail in a 15 cycle PCR reaction. The products were purified AMPure XP system and quantified using the Agilent high sensitivity DNA assay on a Bioanalyzer 2100 system Agilent. The sequencing libraries were then sequenced at both ends for 150 bp using a Hiseq Xten platform Illumina by Shanghai Personal Biotechnology Cp. Ltd.     RNA-Seq TRANSCRIPTOMIC PCR PAIRED ILLUMINA HiSeq X Ten   SRP166817     24h-3_R1.fastq.gz 24h-3_R2.fastq.gz fastq fastq 6723114940.0 22261970.0 24h 3 R1.fastq.gz 0:151 1:151 A:1833689339;C:1522824611;G:1529534136;T:1836931192;N:135662 151 151     1833689339 1522824611 1529534136 1836931192 135662 SRX4954242 SRS3995634 SRA800477 Chinese Academy of Fishery Sciences|Yangtze River Fisheries Research Institute Chinese Academy of Fishery Sciences 2 0.91356 0.91347 0.13955 0.14059 0.73898 0.74399 0.49776 0.50087 151 151 B B biological fallback assumption illumina hiseq_era unknown random_priming trueseq bulk unknown unknown   China 2019-01-01 Undetermined Undetermined Cell Line Cell Line
49836 49836 SRR8133151 SRX4954241 SRS3995633 SRP166817 PRJNA498018 Danio rerio strain:ZFL cell line Genome sequencing PRJNA498018 Whole Genome Sequencing Transcriptional responses of ZFL exposed to microcystin LR       sample exposed to microcystin for xxx hour replicate #3 12h 3   isolate:ZFL cell line|dev stage:not applicable|sex:not applicable|tissue:cultured cell line|cell line:ZFL|cell type:epithelia|treatment:exposed to microcystin for xxx hour replicate #3|BioSampleModel:Model organism or animal                 sample exposed to microcystin for xxx hour replicate #3 12h 3 12h 3 Sequencing libraries were generated using the TruSeq RNA Sample Preparation Kit Illumina according to manufacturers instructions. Three micrograms of total RNA were used as initial material for library preparation. Briefly mRNA was purified using poly T oligo attached magnetic beads. Fragmentation of mRNA was conducted using divalent cations under elevated temperature in the Illumina proprietary fragmentation buffer. First strand cDNA was synthesized using random oligonucleotides and SuperScript II. Second strand cDNA was subsequently synthesized using DNA Polymerase I and RNase H. post adenylation of the 3 ends of DNA fragments Illumina PE adapter oligonucleotides were ligated and the library fragments were purified using the AMPure XP system Beckman Coulter. DNA fragments with ligated adaptors on both ends were selectively enriched using Illumina PCR Primer Cocktail in a 15 cycle PCR reaction. The products were purified AMPure XP system and quantified using the Agilent high sensitivity DNA assay on a Bioanalyzer 2100 system Agilent. The sequencing libraries were then sequenced at both ends for 150 bp using a Hiseq Xten platform Illumina by Shanghai Personal Biotechnology Cp. Ltd.     RNA-Seq TRANSCRIPTOMIC PCR PAIRED ILLUMINA HiSeq X Ten   SRP166817     12h-3_R1.fastq.gz 12h-3_R2.fastq.gz fastq fastq 6249709404.0 20694402.0 12h 3 R1.fastq.gz 0:151 1:151 A:1679876276;C:1439210018;G:1449953029;T:1680544537;N:125544 151 151     1679876276 1439210018 1449953029 1680544537 125544 SRX4954241 SRS3995633 SRA800476 Chinese Academy of Fishery Sciences|Yangtze River Fisheries Research Institute Chinese Academy of Fishery Sciences 2 0.9318 0.93185 0.10315 0.10397 0.7499 0.75513 0.49669 0.50516 151 151 B B biological fallback assumption illumina hiseq_era unknown random_priming trueseq bulk unknown unknown   China 2019-01-01 Undetermined Undetermined Cell Line Cell Line
49837 49837 SRR8133152 SRX4954240 SRS3995632 SRP166817 PRJNA498018 Danio rerio strain:ZFL cell line Genome sequencing PRJNA498018 Whole Genome Sequencing Transcriptional responses of ZFL exposed to microcystin LR       sample exposed to microcystin for xxx hour replicate #1 12h 1   isolate:ZFL cell line|dev stage:not applicable|sex:not applicable|tissue:cultured cell line|cell line:ZFL|cell type:epithelia|treatment:exposed to microcystin for xxx hour replicate #1|BioSampleModel:Model organism or animal                 sample exposed to microcystin for xxx hour replicate #1 12h 1 12h 1 Sequencing libraries were generated using the TruSeq RNA Sample Preparation Kit Illumina according to manufacturers instructions. Three micrograms of total RNA were used as initial material for library preparation. Briefly mRNA was purified using poly T oligo attached magnetic beads. Fragmentation of mRNA was conducted using divalent cations under elevated temperature in the Illumina proprietary fragmentation buffer. First strand cDNA was synthesized using random oligonucleotides and SuperScript II. Second strand cDNA was subsequently synthesized using DNA Polymerase I and RNase H. post adenylation of the 3 ends of DNA fragments Illumina PE adapter oligonucleotides were ligated and the library fragments were purified using the AMPure XP system Beckman Coulter. DNA fragments with ligated adaptors on both ends were selectively enriched using Illumina PCR Primer Cocktail in a 15 cycle PCR reaction. The products were purified AMPure XP system and quantified using the Agilent high sensitivity DNA assay on a Bioanalyzer 2100 system Agilent. The sequencing libraries were then sequenced at both ends for 150 bp using a Hiseq Xten platform Illumina by Shanghai Personal Biotechnology Cp. Ltd.     RNA-Seq TRANSCRIPTOMIC PCR PAIRED ILLUMINA HiSeq X Ten   SRP166817     12h-1_R1.fastq.gz 12h-1_R2.fastq.gz fastq fastq 6040713928.0 20002364.0 12h 1 R1.fastq.gz 0:151 1:151 A:1604304459;C:1407301760;G:1430569355;T:1597934265;N:604089 151 151     1604304459 1407301760 1430569355 1597934265 604089 SRX4954240 SRS3995632 SRA800476 Chinese Academy of Fishery Sciences|Yangtze River Fisheries Research Institute Chinese Academy of Fishery Sciences 2 0.93665 0.93821 0.09301 0.0957 0.75284 0.77169 0.49115 0.48606 151 151 B B biological fallback assumption illumina hiseq_era unknown random_priming trueseq bulk unknown unknown   China 2019-01-01 Undetermined Undetermined Cell Line Cell Line
49838 49838 SRR8133153 SRX4954239 SRS3995631 SRP166817 PRJNA498018 Danio rerio strain:ZFL cell line Genome sequencing PRJNA498018 Whole Genome Sequencing Transcriptional responses of ZFL exposed to microcystin LR       sample exposed to microcystin for xxx hour replicate #2 12h 2   isolate:ZFL cell line|dev stage:not applicable|sex:not applicable|tissue:cultured cell line|cell line:ZFL|cell type:epithelia|treatment:exposed to microcystin for xxx hour replicate #2|BioSampleModel:Model organism or animal                 sample exposed to microcystin for xxx hour replicate #2 12h 2 12h 2 Sequencing libraries were generated using the TruSeq RNA Sample Preparation Kit Illumina according to manufacturers instructions. Three micrograms of total RNA were used as initial material for library preparation. Briefly mRNA was purified using poly T oligo attached magnetic beads. Fragmentation of mRNA was conducted using divalent cations under elevated temperature in the Illumina proprietary fragmentation buffer. First strand cDNA was synthesized using random oligonucleotides and SuperScript II. Second strand cDNA was subsequently synthesized using DNA Polymerase I and RNase H. post adenylation of the 3 ends of DNA fragments Illumina PE adapter oligonucleotides were ligated and the library fragments were purified using the AMPure XP system Beckman Coulter. DNA fragments with ligated adaptors on both ends were selectively enriched using Illumina PCR Primer Cocktail in a 15 cycle PCR reaction. The products were purified AMPure XP system and quantified using the Agilent high sensitivity DNA assay on a Bioanalyzer 2100 system Agilent. The sequencing libraries were then sequenced at both ends for 150 bp using a Hiseq Xten platform Illumina by Shanghai Personal Biotechnology Cp. Ltd.     RNA-Seq TRANSCRIPTOMIC PCR PAIRED ILLUMINA HiSeq X Ten   SRP166817     12h-2_R1.fastq.gz 12h-2_R2.fastq.gz fastq fastq 6340973200.0 20996600.0 12h 2 R1.fastq.gz 0:151 1:151 A:1704338617;C:1461869913;G:1470728810;T:1703906206;N:129654 151 151     1704338617 1461869913 1470728810 1703906206 129654 SRX4954239 SRS3995631 SRA800476 Chinese Academy of Fishery Sciences|Yangtze River Fisheries Research Institute Chinese Academy of Fishery Sciences 2 0.93272 0.93343 0.10216 0.10338 0.74978 0.75513 0.50094 0.51045 151 151 B B biological fallback assumption illumina hiseq_era unknown random_priming trueseq bulk unknown unknown   China 2019-01-01 Undetermined Undetermined Cell Line Cell Line
49839 49839 SRR8132757 SRX4953863 SRS3995402 SRP166817 PRJNA498018 Danio rerio strain:ZFL cell line Genome sequencing PRJNA498018 Whole Genome Sequencing Transcriptional responses of ZFL exposed to microcystin LR       sample exposed to microcystin for xxx hour replicate #3 6h 3   isolate:ZFL cell line|dev stage:not applicable|sex:not applicable|tissue:cultured cell line|cell line:ZFL|cell type:epithelia|treatment:exposed to microcystin for xxx hour replicate #3|BioSampleModel:Model organism or animal                 sample exposed to microcystin for xxx hour replicate #3 6h 3 6h 3 Sequencing libraries were generated using the TruSeq RNA Sample Preparation Kit Illumina according to manufacturers instructions. Three micrograms of total RNA were used as initial material for library preparation. Briefly mRNA was purified using poly T oligo attached magnetic beads. Fragmentation of mRNA was conducted using divalent cations under elevated temperature in the Illumina proprietary fragmentation buffer. First strand cDNA was synthesized using random oligonucleotides and SuperScript II. Second strand cDNA was subsequently synthesized using DNA Polymerase I and RNase H. post adenylation of the 3 ends of DNA fragments Illumina PE adapter oligonucleotides were ligated and the library fragments were purified using the AMPure XP system Beckman Coulter. DNA fragments with ligated adaptors on both ends were selectively enriched using Illumina PCR Primer Cocktail in a 15 cycle PCR reaction. The products were purified AMPure XP system and quantified using the Agilent high sensitivity DNA assay on a Bioanalyzer 2100 system Agilent. The sequencing libraries were then sequenced at both ends for 150 bp using a Hiseq Xten platform Illumina by Shanghai Personal Biotechnology Cp. Ltd.     RNA-Seq TRANSCRIPTOMIC PCR PAIRED ILLUMINA HiSeq X Ten   SRP166817     6h-3_R1.fastq.gz 6h-3_R2.fastq.gz fastq fastq 6533368340.0 21633670.0 6h 3 R1.fastq.gz 0:151 1:151 A:1684379253;C:1575728468;G:1593737205;T:1678875387;N:648027 151 151     1684379253 1575728468 1593737205 1678875387 648027 SRX4953863 SRS3995402 SRA800451 Chinese Academy of Fishery Sciences|Yangtze River Fisheries Research Institute Chinese Academy of Fishery Sciences 2 0.94744 0.94959 0.06681 0.06774 0.76459 0.77711 0.4873 0.49779 151 151 B B biological fallback assumption illumina hiseq_era unknown random_priming trueseq bulk unknown unknown   China 2019-01-01 Undetermined Undetermined Cell Line Cell Line
49840 49840 SRR8132756 SRX4953862 SRS3995403 SRP166817 PRJNA498018 Danio rerio strain:ZFL cell line Genome sequencing PRJNA498018 Whole Genome Sequencing Transcriptional responses of ZFL exposed to microcystin LR       sample exposed to microcystin for xxx hour replicate #1 6h 1   isolate:ZFL cell line|dev stage:not applicable|sex:not applicable|tissue:cultured cell line|cell line:ZFL|cell type:epithelia|treatment:exposed to microcystin for xxx hour replicate #1|BioSampleModel:Model organism or animal                 sample exposed to microcystin for xxx hour replicate #1 6h 1 6h 1 Sequencing libraries were generated using the TruSeq RNA Sample Preparation Kit Illumina according to manufacturers instructions. Three micrograms of total RNA were used as initial material for library preparation. Briefly mRNA was purified using poly T oligo attached magnetic beads. Fragmentation of mRNA was conducted using divalent cations under elevated temperature in the Illumina proprietary fragmentation buffer. First strand cDNA was synthesized using random oligonucleotides and SuperScript II. Second strand cDNA was subsequently synthesized using DNA Polymerase I and RNase H. post adenylation of the 3 ends of DNA fragments Illumina PE adapter oligonucleotides were ligated and the library fragments were purified using the AMPure XP system Beckman Coulter. DNA fragments with ligated adaptors on both ends were selectively enriched using Illumina PCR Primer Cocktail in a 15 cycle PCR reaction. The products were purified AMPure XP system and quantified using the Agilent high sensitivity DNA assay on a Bioanalyzer 2100 system Agilent. The sequencing libraries were then sequenced at both ends for 150 bp using a Hiseq Xten platform Illumina by Shanghai Personal Biotechnology Cp. Ltd.     RNA-Seq TRANSCRIPTOMIC PCR PAIRED ILLUMINA HiSeq X Ten   SRP166817     6h-1_R1.fastq.gz 6h-1_R2.fastq.gz fastq fastq 6686156482.0 22139591.0 6h 1 R1.fastq.gz 0:151 1:151 A:1712757343;C:1622803789;G:1646819107;T:1703094369;N:681874 151 151     1712757343 1622803789 1646819107 1703094369 681874 SRX4953862 SRS3995403 SRA800449 Chinese Academy of Fishery Sciences|Yangtze River Fisheries Research Institute Chinese Academy of Fishery Sciences 2 0.95018 0.95234 0.0661 0.06774 0.77268 0.78652 0.49287 0.48663 151 151 B B biological fallback assumption illumina hiseq_era unknown random_priming trueseq bulk unknown unknown   China 2018-10-29 Undetermined Undetermined Cell Line Cell Line
49841 49841 SRR8132755 SRX4953861 SRS3995401 SRP166817 PRJNA498018 Danio rerio strain:ZFL cell line Genome sequencing PRJNA498018 Whole Genome Sequencing Transcriptional responses of ZFL exposed to microcystin LR       sample exposed to microcystin for xxx hour replicate #2 6h 2   isolate:ZFL cell line|dev stage:not applicable|sex:not applicable|tissue:cultured cell line|cell line:ZFL|cell type:epithelia|treatment:exposed to microcystin for xxx hour replicate #2|BioSampleModel:Model organism or animal                 sample exposed to microcystin for xxx hour replicate #2 6h 2 6h 2 Sequencing libraries were generated using the TruSeq RNA Sample Preparation Kit Illumina according to manufacturers instructions. Three micrograms of total RNA were used as initial material for library preparation. Briefly mRNA was purified using poly T oligo attached magnetic beads. Fragmentation of mRNA was conducted using divalent cations under elevated temperature in the Illumina proprietary fragmentation buffer. First strand cDNA was synthesized using random oligonucleotides and SuperScript II. Second strand cDNA was subsequently synthesized using DNA Polymerase I and RNase H. post adenylation of the 3 ends of DNA fragments Illumina PE adapter oligonucleotides were ligated and the library fragments were purified using the AMPure XP system Beckman Coulter. DNA fragments with ligated adaptors on both ends were selectively enriched using Illumina PCR Primer Cocktail in a 15 cycle PCR reaction. The products were purified AMPure XP system and quantified using the Agilent high sensitivity DNA assay on a Bioanalyzer 2100 system Agilent. The sequencing libraries were then sequenced at both ends for 150 bp using a Hiseq Xten platform Illumina by Shanghai Personal Biotechnology Cp. Ltd.     RNA-Seq TRANSCRIPTOMIC PCR PAIRED ILLUMINA HiSeq X Ten   SRP166817     6h-2_R1.fastq.gz 6h-2_R2.fastq.gz fastq fastq 5551304204.0 18381802.0 6h 2 R1.fastq.gz 0:151 1:151 A:1408740987;C:1361721973;G:1380328727;T:1399965481;N:547036 151 151     1408740987 1361721973 1380328727 1399965481 547036 SRX4953861 SRS3995401 SRA800450 Chinese Academy of Fishery Sciences|Yangtze River Fisheries Research Institute Chinese Academy of Fishery Sciences 2 0.9506 0.95313 0.07031 0.07155 0.7739 0.78591 0.50125 0.49851 151 151 B B biological fallback assumption illumina hiseq_era unknown random_priming trueseq bulk unknown unknown   China 2018-10-29 Undetermined Undetermined Cell Line Cell Line
49842 49842 SRR8119911 SRX4946208 SRS3988532 SRP166817 PRJNA498018 Danio rerio strain:ZFL cell line Genome sequencing PRJNA498018 Whole Genome Sequencing Transcriptional responses of ZFL exposed to microcystin LR       sample exposed to microcystin for xxx hour replicate #3 3h 3   isolate:ZFL cell line|dev stage:not applicable|sex:not applicable|tissue:cultured cell line|cell line:ZFL|cell type:epithelia|treatment:exposed to microcystin for xxx hour replicate #3|BioSampleModel:Model organism or animal                 sample exposed to microcystin for xxx hour replicate #3 3h 3 3h 3 Sequencing libraries were generated using the TruSeq RNA Sample Preparation Kit Illumina according to manufacturers instructions. Three micrograms of total RNA were used as initial material for library preparation. Briefly mRNA was purified using poly T oligo attached magnetic beads. Fragmentation of mRNA was conducted using divalent cations under elevated temperature in the Illumina proprietary fragmentation buffer. First strand cDNA was synthesized using random oligonucleotides and SuperScript II. Second strand cDNA was subsequently synthesized using DNA Polymerase I and RNase H. post adenylation of the 3 ends of DNA fragments Illumina PE adapter oligonucleotides were ligated and the library fragments were purified using the AMPure XP system Beckman Coulter. DNA fragments with ligated adaptors on both ends were selectively enriched using Illumina PCR Primer Cocktail in a 15 cycle PCR reaction. The products were purified AMPure XP system and quantified using the Agilent high sensitivity DNA assay on a Bioanalyzer 2100 system Agilent. The sequencing libraries were then sequenced at both ends for 150 bp using a Hiseq Xten platform Illumina by Shanghai Personal Biotechnology Cp. Ltd.     RNA-Seq TRANSCRIPTOMIC PCR PAIRED ILLUMINA HiSeq X Ten   SRP166817     3h-3_R1.fastq.gz 3h-3_R2.fastq.gz fastq fastq 6249422202.0 20693451.0 3h 3 R1.fastq.gz 0:151 1:151 A:1700610088;C:1416827975;G:1436930329;T:1694433965;N:619845 151 151     1700610088 1416827975 1436930329 1694433965 619845 SRX4946208 SRS3988532 SRA799983 Chinese Academy of Fishery Sciences|Yangtze River Fisheries Research Institute Chinese Academy of Fishery Sciences 2 0.924 0.92633 0.13412 0.13864 0.74158 0.76489 0.49551 0.49903 151 151 B B biological fallback assumption illumina hiseq_era unknown random_priming trueseq bulk unknown unknown   China 2018-10-29 Undetermined Undetermined Cell Line Cell Line
49843 49843 SRR8119910 SRX4946207 SRS3988531 SRP166817 PRJNA498018 Danio rerio strain:ZFL cell line Genome sequencing PRJNA498018 Whole Genome Sequencing Transcriptional responses of ZFL exposed to microcystin LR       sample exposed to microcystin for xxx hour replicate #2 3h 2   isolate:ZFL cell line|dev stage:not applicable|sex:not applicable|tissue:cultured cell line|cell line:ZFL|cell type:epithelia|treatment:exposed to microcystin for xxx hour replicate #2|BioSampleModel:Model organism or animal                 sample exposed to microcystin for xxx hour replicate #2 3h 2 3h 2 Sequencing libraries were generated using the TruSeq RNA Sample Preparation Kit Illumina according to manufacturers instructions. Three micrograms of total RNA were used as initial material for library preparation. Briefly mRNA was purified using poly T oligo attached magnetic beads. Fragmentation of mRNA was conducted using divalent cations under elevated temperature in the Illumina proprietary fragmentation buffer. First strand cDNA was synthesized using random oligonucleotides and SuperScript II. Second strand cDNA was subsequently synthesized using DNA Polymerase I and RNase H. post adenylation of the 3 ends of DNA fragments Illumina PE adapter oligonucleotides were ligated and the library fragments were purified using the AMPure XP system Beckman Coulter. DNA fragments with ligated adaptors on both ends were selectively enriched using Illumina PCR Primer Cocktail in a 15 cycle PCR reaction. The products were purified AMPure XP system and quantified using the Agilent high sensitivity DNA assay on a Bioanalyzer 2100 system Agilent. The sequencing libraries were then sequenced at both ends for 150 bp using a Hiseq Xten platform Illumina by Shanghai Personal Biotechnology Cp. Ltd.     RNA-Seq TRANSCRIPTOMIC PCR PAIRED ILLUMINA HiSeq X Ten   SRP166817     3h-2_R1.fastq.gz 3h-2_R2.fastq.gz fastq fastq 6691460508.0 22157154.0 3h 2 R2.fastq.gz 0:151 1:151 A:1817827404;C:1520902100;G:1534921644;T:1817122657;N:686703 151 151     1817827404 1520902100 1534921644 1817122657 686703 SRX4946207 SRS3988531 SRA799982 Chinese Academy of Fishery Sciences|Yangtze River Fisheries Research Institute Chinese Academy of Fishery Sciences 2 0.92541 0.92734 0.13003 0.1321 0.7443 0.76019 0.50143 0.4998 151 151 B B biological fallback assumption illumina hiseq_era unknown random_priming trueseq bulk unknown unknown   China 2019-01-01 Undetermined Undetermined Cell Line Cell Line
49844 49844 SRR8117643 SRX4943940 SRS3986328 SRP166817 PRJNA498018 Danio rerio strain:ZFL cell line Genome sequencing PRJNA498018 Whole Genome Sequencing Transcriptional responses of ZFL exposed to microcystin LR       sample exposed to microcystin for xxx hour replicate #1 3h 1   isolate:ZFL cell line|dev stage:not applicable|sex:not applicable|tissue:cultured cell line|cell line:ZFL|cell type:epithelia|treatment:exposed to microcystin for xxx hour replicate #1|BioSampleModel:Model organism or animal                 sample exposed to microcystin for xxx hour replicate #1 3h 1 3h 1 Sequencing libraries were generated using the TruSeq RNA Sample Preparation Kit Illumina according to manufacturers instructions. Three micrograms of total RNA were used as initial material for library preparation. Briefly mRNA was purified using poly T oligo attached magnetic beads. Fragmentation of mRNA was conducted using divalent cations under elevated temperature in the Illumina proprietary fragmentation buffer. First strand cDNA was synthesized using random oligonucleotides and SuperScript II. Second strand cDNA was subsequently synthesized using DNA Polymerase I and RNase H. post adenylation of the 3 ends of DNA fragments Illumina PE adapter oligonucleotides were ligated and the library fragments were purified using the AMPure XP system Beckman Coulter. DNA fragments with ligated adaptors on both ends were selectively enriched using Illumina PCR Primer Cocktail in a 15 cycle PCR reaction. The products were purified AMPure XP system and quantified using the Agilent high sensitivity DNA assay on a Bioanalyzer 2100 system Agilent. The sequencing libraries were then sequenced at both ends for 150 bp using a Hiseq Xten platform Illumina by Shanghai Personal Biotechnology Cp. Ltd.     RNA-Seq TRANSCRIPTOMIC PCR PAIRED ILLUMINA HiSeq X Ten   SRP166817     3h-1_R1.fastq.gz 3h-1_R2.fastq.gz fastq fastq 6151798890.0 20370195.0 3h 1 R1.fastq.gz 0:151 1:151 A:1649142984;C:1419068750;G:1435991843;T:1646989815;N:605498 151 151     1649142984 1419068750 1435991843 1646989815 605498 SRX4943940 SRS3986328 SRA799824 Chinese Academy of Fishery Sciences|Yangtze River Fisheries Research Institute Chinese Academy of Fishery Sciences 2 0.92965 0.93287 0.12221 0.12677 0.73397 0.75278 0.50535 0.50378 151 151 B B biological fallback assumption illumina hiseq_era unknown random_priming trueseq bulk unknown unknown   China 2018-10-28 Undetermined Undetermined Cell Line Cell Line

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CREATE TABLE run_metadata("run.accession" VARCHAR, "experiment.accession" VARCHAR, "sample.accession" VARCHAR, "study.accession" VARCHAR, bioproject VARCHAR, "study.title" VARCHAR, "study.alias" VARCHAR, "study.type" VARCHAR, "study.abstract" VARCHAR, "study.attributes" VARCHAR, "study.PMIDs" VARCHAR, "sample.description" VARCHAR, "sample.title" VARCHAR, "sample.alias" VARCHAR, "sample.centername" VARCHAR, "sample.attributes" VARCHAR, "GEOsample.title" VARCHAR, "GEOsample.dataprocessing" VARCHAR, "GEOsample.source" VARCHAR, "GEOsample.treatmentprotocol" VARCHAR, "GEOsample.extractprotocol" VARCHAR, "GEOsample.growthprotocol" VARCHAR, "GEOsample.characteristics" VARCHAR, "GEOsample.accession" VARCHAR, "experiment.title" VARCHAR, "experiment.alias" VARCHAR, "experiment.library_name" VARCHAR, "experiment.design_description" VARCHAR, "experiment.library_construction_protocol" VARCHAR, "experiment.attributes" VARCHAR, "experiment.library_strategy" VARCHAR, "experiment.library_source" VARCHAR, "experiment.library_selection" VARCHAR, "experiment.library_layout" VARCHAR, "experiment.platform" VARCHAR, "experiment.instrument_model" VARCHAR, "experiment.spot_descriptor" VARCHAR, "experiment.study_ref" VARCHAR, "run.title" VARCHAR, "run.attributes" VARCHAR, "run.filename" VARCHAR, "run.semantic_name" VARCHAR, "run.total_bases" DOUBLE, "run.total_spots" DOUBLE, "run.alias" VARCHAR, "run.read_lengths" VARCHAR, "run.base_counts" VARCHAR, "run.r1_length" BIGINT, "run.r2_length" BIGINT, "run.r3_length" BIGINT, "run.r4_length" BIGINT, "run.Acount" BIGINT, "run.Ccount" BIGINT, "run.Gcount" BIGINT, "run.Tcount" BIGINT, "run.Ncount" BIGINT, "run.experiment" VARCHAR, "run.pool_member" VARCHAR, "submission.accession" VARCHAR, "submission.srasource" VARCHAR, "submission.bioprojectsource" VARCHAR, "seqdetective.n_mates" BIGINT, "seqdetective.mapping_rate.mate1" DOUBLE, "seqdetective.mapping_rate.mate2" DOUBLE, "seqdetective.nofeature_rate.mate1" DOUBLE, "seqdetective.nofeature_rate.mate2" DOUBLE, "seqdetective.sparsity.mate1" DOUBLE, "seqdetective.sparsity.mate2" DOUBLE, "seqdetective.pos_strand_rate.mate1" DOUBLE, "seqdetective.pos_strand_rate.mate2" DOUBLE, "seqdetective.readlen.mate1" BIGINT, "seqdetective.readlen.mate2" BIGINT, "seqdetective.judgement.mate1" VARCHAR, "seqdetective.judgement.mate2" VARCHAR, "seqdetective.judgement.reason" VARCHAR, platform_family VARCHAR, instrument_generation VARCHAR, read_bias VARCHAR, selection_class VARCHAR, prep_kit VARCHAR, sc_or_bulk VARCHAR, tech_class VARCHAR, technology VARCHAR, tech_variant VARCHAR, "submission.bioprojectsource.country" VARCHAR, earliest_date DATE, devstage_curation VARCHAR, devstage_curation_coarse VARCHAR, tissue_curation VARCHAR, tissue_curation_coarse VARCHAR);;
CREATE INDEX idx_run_bioproject ON run_metadata(bioproject);;
CREATE INDEX idx_run_run_accession ON run_metadata("run.accession");;