run_metadata
144 rows where devstage_curation = "Pharyngula" and experiment.library_selection = "Oligo-dT"
This data as json, CSV (advanced)
| Link | rowid ▼ | run.accession | experiment.accession | sample.accession | study.accession | bioproject | study.title | study.alias | study.type | study.abstract | study.attributes | study.PMIDs | sample.description | sample.title | sample.alias | sample.centername | sample.attributes | GEOsample.title | GEOsample.dataprocessing | GEOsample.source | GEOsample.treatmentprotocol | GEOsample.extractprotocol | GEOsample.growthprotocol | GEOsample.characteristics | GEOsample.accession | experiment.title | experiment.alias | experiment.library_name | experiment.design_description | experiment.library_construction_protocol | experiment.attributes | experiment.library_strategy | experiment.library_source | experiment.library_selection | experiment.library_layout | experiment.platform | experiment.instrument_model | experiment.spot_descriptor | experiment.study_ref | run.title | run.attributes | run.filename | run.semantic_name | run.total_bases | run.total_spots | run.alias | run.read_lengths | run.base_counts | run.r1_length | run.r2_length | run.r3_length | run.r4_length | run.Acount | run.Ccount | run.Gcount | run.Tcount | run.Ncount | run.experiment | run.pool_member | submission.accession | submission.srasource | submission.bioprojectsource | seqdetective.n_mates | seqdetective.mapping_rate.mate1 | seqdetective.mapping_rate.mate2 | seqdetective.nofeature_rate.mate1 | seqdetective.nofeature_rate.mate2 | seqdetective.sparsity.mate1 | seqdetective.sparsity.mate2 | seqdetective.pos_strand_rate.mate1 | seqdetective.pos_strand_rate.mate2 | seqdetective.readlen.mate1 | seqdetective.readlen.mate2 | seqdetective.judgement.mate1 | seqdetective.judgement.mate2 | seqdetective.judgement.reason | platform_family | instrument_generation | read_bias | selection_class | prep_kit | sc_or_bulk | tech_class | technology | tech_variant | submission.bioprojectsource.country | earliest_date | devstage_curation | devstage_curation_coarse | tissue_curation | tissue_curation_coarse |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 11753 | 11753 | ERR11758614 | ERX11157721 | ERS16172939 | ERP149744 | PRJEB64563 | Single cell RNA seq 10x Chromium of FACS sorted drl:H2B Dendra2 etv2:Kaede fli1:DsRed and tp1:eGFP transgenic zebrafish embryos | E-MTAB-13196 | Transcriptome Analysis | Development of the dorsal aorta is a key step in the establishment of the adult blood forming system since hematopoietic stem and progenitor cells HSPCs arise from ventral aortic endothelium in all vertebrate animals studied. Work in zebrafish has demonstrated that arterial and venous endothelial precursors arise from distinct subsets of lateral plate mesoderm. Here we profile the transcriptome of the earliest detectable endothelial cells ECs during zebrafish embryogenesis to demonstrate that tissue specific EC programs initiate much earlier than previously appreciated by the end of gastrulation. Classic studies in the chick embryo showed that paraxial mesoderm generates a subset of somite derived endothelial cells SDECs that incorporate into the dorsal aorta to replace HSPCs as they exit the aorta and enter circulation. We describe a conserved program in the zebrafish where a rare population of endothelial precursors delaminates from the dermomyotome to incorporate exclusively into the developing dorsal aorta. Whereas SDECs lack hematopoietic potential they act as a local niche to support the emergence of HSPCs from neighboring hemogenic endothelium. Thus at least three subsets of ECs contribute to the developing dorsal aorta: vascular ECs hemogenic ECs and SDECs. Taken together our findings indicate that the distinct spatial origins of endothelial precursors dictate different cellular potentials within the developing dorsal aorta. | ENA FIRST PUBLIC:2023 08 01|ENA LAST UPDATE:2023 08 01 | Protocols: Dechorionated embryos were collected at defined stages dissociated by pipetting centrifugation at 300g for 5 minutes washed once with PBS and sorted by FACS. Total cell concentration and viability were determined using a TC20 Automated Cell Counter Bio Rad. Samples were then re suspended in 1x PBS with 0.05% BSA at 800 3000 cells per ml. Samples were loaded on the 10x Chromium system with a target of 4 000 cells. Chromium Single Cell 3ʹ GEM Library & Gel Bead Kit v2 or v3 was used for library preparation. Samples were loaded on the 10x Chromium system with a target of 4 000 cells. Chromium Single Cell 3ʹ GEM Library & Gel Bead Kit v2 or v3 was used for library preparation. | fli1tp1 24hpf | SAMEA114192246 | Institute of Molecular Genetics of the Czech Academy of Sciences | ENA FIRST PUBLIC:2023 08 01T00:17:16Z|ENA LAST UPDATE:2023 08 01T00:17:16Z|External Id:SAMEA114192246|INSDC center alias:Institute of Molecular Genetics of the Czech Academy of Sciences|INSDC center name:Institute of Molecular Genetics of the Czech Academy of Sciences|INSDC first public:2023 08 01T00:17:16Z|INSDC last update:2023 08 01T00:17:16Z|INSDC status:public|Submitter Id:E MTAB 13196:fli1tp1 24hpf|age:24|broker name:ArrayExpress|cell type:endothelial cell|collection date:not collected|common name:zebrafish|developmental stage:24 hpf|genotype:fli1:DsRed tp1:eGFP|geographic location country and/or sea:not collected|immunophenotype:fli1:DsRed+ tp1:eGFP+|isolate:not applicable|organism part:embryo|sample name:E MTAB 13196:fli1tp1 24hpf|scientific name:Danio rerio|strain:fli1:DsRed tp1:eGFP | Illumina HiSeq 2500 paired end sequencing; Single cell RNA seq 10x Chromium of FACS sorted drl:H2B Dendra2 etv2:Kaede fli1:DsRed and tp1:eGFP transgenic zebrafish embryos | E MTAB 13196:fli1tp1 24hpf p | fli1tp1 24hpf p | Single cell RNA seq 10x Chromium of FACS sorted drl:H2B Dendra2 etv2:Kaede fli1:DsRed and tp1:eGFP transgenic zebrafish embryos | Dechorionated embryos were collected at defined stages dissociated by pipetting centrifugation at 300g for 5 minutes washed once with PBS and sorted by FACS. Total cell concentration and viability were determined using a TC20 Automated Cell Counter Bio Rad. Samples were then re suspended in 1x PBS with 0.05% BSA at 800 3000 cells per ml. Samples were loaded on the 10x Chromium system with a target of 4 000 cells. Chromium Single Cell 3สน GEM Library & Gel Bead Kit v2 or v3 was used for library preparation. Samples were loaded on the 10x Chromium system with a target of 4 000 cells. Chromium Single Cell 3สน GEM Library & Gel Bead Kit v2 or v3 was used for library preparation. | RNA-Seq | TRANSCRIPTOMIC SINGLE CELL | Oligo-dT | PAIRED | ILLUMINA | Illumina HiSeq 2500 | ERP149744 | Illumina HiSeq 2500 paired end sequencing; Single cell RNA seq 10x Chromium of FACS sorted drl:H2B Dendra2 etv2:Kaede fli1:DsRed and tp1:eGFP transgenic zebrafish embryos | ENA FIRST PUBLIC:2023 08 01|ENA LAST UPDATE:2023 08 01 | FliTp124hpf_S25_L001_R1_001.fastq.gz FliTp124hpf_S25_L001_R2_001.fastq.gz | fastq fastq | 226833768.0 | 1800268.0 | E MTAB 13196:FliTp124hpf S25 L001 | 0:28 1:98 | A:65319978;C:50920193;G:49999849;T:60474280;N:119468 | 28 | 98 | 65319978 | 50920193 | 49999849 | 60474280 | 119468 | ERX11157721 | ERS16172939 | ERA25628220 | Institute of Molecular Genetics of the Czech Academy of Sciences|European Nucleotide Archive | Institute of Molecular Genetics of the Czech Academy of Sciences|European Nucleotide Archive | 2 | 0.00122 | 0.88236 | 0.00056 | 0.09684 | 0.9978 | 0.89309 | 0.424 | 0.44186 | 28 | 98 | T | B | sc-like readlen | illumina | hiseq_era | unknown | poly_a | unknown | sc | single_cell_droplet | 10x | Czech Republic | 2023-08-01 | Pharyngula | Embryo | Embryo Imprecise | All anatomical structures | ||||||||||||||
| 11754 | 11754 | ERR11758619 | ERX11157721 | ERS16172939 | ERP149744 | PRJEB64563 | Single cell RNA seq 10x Chromium of FACS sorted drl:H2B Dendra2 etv2:Kaede fli1:DsRed and tp1:eGFP transgenic zebrafish embryos | E-MTAB-13196 | Transcriptome Analysis | Development of the dorsal aorta is a key step in the establishment of the adult blood forming system since hematopoietic stem and progenitor cells HSPCs arise from ventral aortic endothelium in all vertebrate animals studied. Work in zebrafish has demonstrated that arterial and venous endothelial precursors arise from distinct subsets of lateral plate mesoderm. Here we profile the transcriptome of the earliest detectable endothelial cells ECs during zebrafish embryogenesis to demonstrate that tissue specific EC programs initiate much earlier than previously appreciated by the end of gastrulation. Classic studies in the chick embryo showed that paraxial mesoderm generates a subset of somite derived endothelial cells SDECs that incorporate into the dorsal aorta to replace HSPCs as they exit the aorta and enter circulation. We describe a conserved program in the zebrafish where a rare population of endothelial precursors delaminates from the dermomyotome to incorporate exclusively into the developing dorsal aorta. Whereas SDECs lack hematopoietic potential they act as a local niche to support the emergence of HSPCs from neighboring hemogenic endothelium. Thus at least three subsets of ECs contribute to the developing dorsal aorta: vascular ECs hemogenic ECs and SDECs. Taken together our findings indicate that the distinct spatial origins of endothelial precursors dictate different cellular potentials within the developing dorsal aorta. | ENA FIRST PUBLIC:2023 08 01|ENA LAST UPDATE:2023 08 01 | Protocols: Dechorionated embryos were collected at defined stages dissociated by pipetting centrifugation at 300g for 5 minutes washed once with PBS and sorted by FACS. Total cell concentration and viability were determined using a TC20 Automated Cell Counter Bio Rad. Samples were then re suspended in 1x PBS with 0.05% BSA at 800 3000 cells per ml. Samples were loaded on the 10x Chromium system with a target of 4 000 cells. Chromium Single Cell 3ʹ GEM Library & Gel Bead Kit v2 or v3 was used for library preparation. Samples were loaded on the 10x Chromium system with a target of 4 000 cells. Chromium Single Cell 3ʹ GEM Library & Gel Bead Kit v2 or v3 was used for library preparation. | fli1tp1 24hpf | SAMEA114192246 | Institute of Molecular Genetics of the Czech Academy of Sciences | ENA FIRST PUBLIC:2023 08 01T00:17:16Z|ENA LAST UPDATE:2023 08 01T00:17:16Z|External Id:SAMEA114192246|INSDC center alias:Institute of Molecular Genetics of the Czech Academy of Sciences|INSDC center name:Institute of Molecular Genetics of the Czech Academy of Sciences|INSDC first public:2023 08 01T00:17:16Z|INSDC last update:2023 08 01T00:17:16Z|INSDC status:public|Submitter Id:E MTAB 13196:fli1tp1 24hpf|age:24|broker name:ArrayExpress|cell type:endothelial cell|collection date:not collected|common name:zebrafish|developmental stage:24 hpf|genotype:fli1:DsRed tp1:eGFP|geographic location country and/or sea:not collected|immunophenotype:fli1:DsRed+ tp1:eGFP+|isolate:not applicable|organism part:embryo|sample name:E MTAB 13196:fli1tp1 24hpf|scientific name:Danio rerio|strain:fli1:DsRed tp1:eGFP | Illumina HiSeq 2500 paired end sequencing; Single cell RNA seq 10x Chromium of FACS sorted drl:H2B Dendra2 etv2:Kaede fli1:DsRed and tp1:eGFP transgenic zebrafish embryos | E MTAB 13196:fli1tp1 24hpf p | fli1tp1 24hpf p | Single cell RNA seq 10x Chromium of FACS sorted drl:H2B Dendra2 etv2:Kaede fli1:DsRed and tp1:eGFP transgenic zebrafish embryos | Dechorionated embryos were collected at defined stages dissociated by pipetting centrifugation at 300g for 5 minutes washed once with PBS and sorted by FACS. Total cell concentration and viability were determined using a TC20 Automated Cell Counter Bio Rad. Samples were then re suspended in 1x PBS with 0.05% BSA at 800 3000 cells per ml. Samples were loaded on the 10x Chromium system with a target of 4 000 cells. Chromium Single Cell 3สน GEM Library & Gel Bead Kit v2 or v3 was used for library preparation. Samples were loaded on the 10x Chromium system with a target of 4 000 cells. Chromium Single Cell 3สน GEM Library & Gel Bead Kit v2 or v3 was used for library preparation. | RNA-Seq | TRANSCRIPTOMIC SINGLE CELL | Oligo-dT | PAIRED | ILLUMINA | Illumina HiSeq 2500 | ERP149744 | Illumina HiSeq 2500 paired end sequencing; Single cell RNA seq 10x Chromium of FACS sorted drl:H2B Dendra2 etv2:Kaede fli1:DsRed and tp1:eGFP transgenic zebrafish embryos | ENA FIRST PUBLIC:2023 08 01|ENA LAST UPDATE:2023 08 01 | FliTp124hpf_S27_L001_R1_001.fastq.gz FliTp124hpf_S27_L001_R2_001.fastq.gz | fastq fastq | 261098334.0 | 2072209.0 | E MTAB 13196:FliTp124hpf S27 L001 | 0:28 1:98 | A:76098436;C:58031830;G:57099955;T:69729759;N:138354 | 28 | 98 | 76098436 | 58031830 | 57099955 | 69729759 | 138354 | ERX11157721 | ERS16172939 | ERA25628220 | Institute of Molecular Genetics of the Czech Academy of Sciences|European Nucleotide Archive | Institute of Molecular Genetics of the Czech Academy of Sciences|European Nucleotide Archive | 2 | 0.00109 | 0.87641 | 0.00062 | 0.0976 | 0.99837 | 0.90065 | 0.45555 | 0.48712 | 28 | 98 | T | B | sc-like readlen | illumina | hiseq_era | unknown | poly_a | unknown | sc | single_cell_droplet | 10x | Czech Republic | 2023-08-01 | Pharyngula | Embryo | Embryo Imprecise | All anatomical structures | ||||||||||||||
| 11755 | 11755 | ERR11758589 | ERX11157721 | ERS16172939 | ERP149744 | PRJEB64563 | Single cell RNA seq 10x Chromium of FACS sorted drl:H2B Dendra2 etv2:Kaede fli1:DsRed and tp1:eGFP transgenic zebrafish embryos | E-MTAB-13196 | Transcriptome Analysis | Development of the dorsal aorta is a key step in the establishment of the adult blood forming system since hematopoietic stem and progenitor cells HSPCs arise from ventral aortic endothelium in all vertebrate animals studied. Work in zebrafish has demonstrated that arterial and venous endothelial precursors arise from distinct subsets of lateral plate mesoderm. Here we profile the transcriptome of the earliest detectable endothelial cells ECs during zebrafish embryogenesis to demonstrate that tissue specific EC programs initiate much earlier than previously appreciated by the end of gastrulation. Classic studies in the chick embryo showed that paraxial mesoderm generates a subset of somite derived endothelial cells SDECs that incorporate into the dorsal aorta to replace HSPCs as they exit the aorta and enter circulation. We describe a conserved program in the zebrafish where a rare population of endothelial precursors delaminates from the dermomyotome to incorporate exclusively into the developing dorsal aorta. Whereas SDECs lack hematopoietic potential they act as a local niche to support the emergence of HSPCs from neighboring hemogenic endothelium. Thus at least three subsets of ECs contribute to the developing dorsal aorta: vascular ECs hemogenic ECs and SDECs. Taken together our findings indicate that the distinct spatial origins of endothelial precursors dictate different cellular potentials within the developing dorsal aorta. | ENA FIRST PUBLIC:2023 08 01|ENA LAST UPDATE:2023 08 01 | Protocols: Dechorionated embryos were collected at defined stages dissociated by pipetting centrifugation at 300g for 5 minutes washed once with PBS and sorted by FACS. Total cell concentration and viability were determined using a TC20 Automated Cell Counter Bio Rad. Samples were then re suspended in 1x PBS with 0.05% BSA at 800 3000 cells per ml. Samples were loaded on the 10x Chromium system with a target of 4 000 cells. Chromium Single Cell 3ʹ GEM Library & Gel Bead Kit v2 or v3 was used for library preparation. Samples were loaded on the 10x Chromium system with a target of 4 000 cells. Chromium Single Cell 3ʹ GEM Library & Gel Bead Kit v2 or v3 was used for library preparation. | fli1tp1 24hpf | SAMEA114192246 | Institute of Molecular Genetics of the Czech Academy of Sciences | ENA FIRST PUBLIC:2023 08 01T00:17:16Z|ENA LAST UPDATE:2023 08 01T00:17:16Z|External Id:SAMEA114192246|INSDC center alias:Institute of Molecular Genetics of the Czech Academy of Sciences|INSDC center name:Institute of Molecular Genetics of the Czech Academy of Sciences|INSDC first public:2023 08 01T00:17:16Z|INSDC last update:2023 08 01T00:17:16Z|INSDC status:public|Submitter Id:E MTAB 13196:fli1tp1 24hpf|age:24|broker name:ArrayExpress|cell type:endothelial cell|collection date:not collected|common name:zebrafish|developmental stage:24 hpf|genotype:fli1:DsRed tp1:eGFP|geographic location country and/or sea:not collected|immunophenotype:fli1:DsRed+ tp1:eGFP+|isolate:not applicable|organism part:embryo|sample name:E MTAB 13196:fli1tp1 24hpf|scientific name:Danio rerio|strain:fli1:DsRed tp1:eGFP | Illumina HiSeq 2500 paired end sequencing; Single cell RNA seq 10x Chromium of FACS sorted drl:H2B Dendra2 etv2:Kaede fli1:DsRed and tp1:eGFP transgenic zebrafish embryos | E MTAB 13196:fli1tp1 24hpf p | fli1tp1 24hpf p | Single cell RNA seq 10x Chromium of FACS sorted drl:H2B Dendra2 etv2:Kaede fli1:DsRed and tp1:eGFP transgenic zebrafish embryos | Dechorionated embryos were collected at defined stages dissociated by pipetting centrifugation at 300g for 5 minutes washed once with PBS and sorted by FACS. Total cell concentration and viability were determined using a TC20 Automated Cell Counter Bio Rad. Samples were then re suspended in 1x PBS with 0.05% BSA at 800 3000 cells per ml. Samples were loaded on the 10x Chromium system with a target of 4 000 cells. Chromium Single Cell 3สน GEM Library & Gel Bead Kit v2 or v3 was used for library preparation. Samples were loaded on the 10x Chromium system with a target of 4 000 cells. Chromium Single Cell 3สน GEM Library & Gel Bead Kit v2 or v3 was used for library preparation. | RNA-Seq | TRANSCRIPTOMIC SINGLE CELL | Oligo-dT | PAIRED | ILLUMINA | Illumina HiSeq 2500 | ERP149744 | Illumina HiSeq 2500 paired end sequencing; Single cell RNA seq 10x Chromium of FACS sorted drl:H2B Dendra2 etv2:Kaede fli1:DsRed and tp1:eGFP transgenic zebrafish embryos | ENA FIRST PUBLIC:2023 08 01|ENA LAST UPDATE:2023 08 01 | FliTp124hpf_S16_L005_R1_001.fastq.gz FliTp124hpf_S16_L005_R2_001.fastq.gz | fastq fastq | 1168367130.0 | 9272755.0 | E MTAB 13196:FliTp124hpf S16 L005 | 0:28 1:98 | A:323651598;C:269884286;G:268030176;T:306695030;N:106040 | 28 | 98 | 323651598 | 269884286 | 268030176 | 306695030 | 106040 | ERX11157721 | ERS16172939 | ERA25628220 | Institute of Molecular Genetics of the Czech Academy of Sciences|European Nucleotide Archive | Institute of Molecular Genetics of the Czech Academy of Sciences|European Nucleotide Archive | 2 | 0.00491 | 0.90853 | 0.00131 | 0.07851 | 0.99019 | 0.84331 | 0.41536 | 0.48076 | 28 | 98 | T | B | sc-like readlen | illumina | hiseq_era | unknown | poly_a | unknown | sc | single_cell_droplet | 10x | Czech Republic | 2023-08-01 | Pharyngula | Embryo | Embryo Imprecise | All anatomical structures | ||||||||||||||
| 11756 | 11756 | ERR11758638 | ERX11157721 | ERS16172939 | ERP149744 | PRJEB64563 | Single cell RNA seq 10x Chromium of FACS sorted drl:H2B Dendra2 etv2:Kaede fli1:DsRed and tp1:eGFP transgenic zebrafish embryos | E-MTAB-13196 | Transcriptome Analysis | Development of the dorsal aorta is a key step in the establishment of the adult blood forming system since hematopoietic stem and progenitor cells HSPCs arise from ventral aortic endothelium in all vertebrate animals studied. Work in zebrafish has demonstrated that arterial and venous endothelial precursors arise from distinct subsets of lateral plate mesoderm. Here we profile the transcriptome of the earliest detectable endothelial cells ECs during zebrafish embryogenesis to demonstrate that tissue specific EC programs initiate much earlier than previously appreciated by the end of gastrulation. Classic studies in the chick embryo showed that paraxial mesoderm generates a subset of somite derived endothelial cells SDECs that incorporate into the dorsal aorta to replace HSPCs as they exit the aorta and enter circulation. We describe a conserved program in the zebrafish where a rare population of endothelial precursors delaminates from the dermomyotome to incorporate exclusively into the developing dorsal aorta. Whereas SDECs lack hematopoietic potential they act as a local niche to support the emergence of HSPCs from neighboring hemogenic endothelium. Thus at least three subsets of ECs contribute to the developing dorsal aorta: vascular ECs hemogenic ECs and SDECs. Taken together our findings indicate that the distinct spatial origins of endothelial precursors dictate different cellular potentials within the developing dorsal aorta. | ENA FIRST PUBLIC:2023 08 01|ENA LAST UPDATE:2023 08 01 | Protocols: Dechorionated embryos were collected at defined stages dissociated by pipetting centrifugation at 300g for 5 minutes washed once with PBS and sorted by FACS. Total cell concentration and viability were determined using a TC20 Automated Cell Counter Bio Rad. Samples were then re suspended in 1x PBS with 0.05% BSA at 800 3000 cells per ml. Samples were loaded on the 10x Chromium system with a target of 4 000 cells. Chromium Single Cell 3ʹ GEM Library & Gel Bead Kit v2 or v3 was used for library preparation. Samples were loaded on the 10x Chromium system with a target of 4 000 cells. Chromium Single Cell 3ʹ GEM Library & Gel Bead Kit v2 or v3 was used for library preparation. | fli1tp1 24hpf | SAMEA114192246 | Institute of Molecular Genetics of the Czech Academy of Sciences | ENA FIRST PUBLIC:2023 08 01T00:17:16Z|ENA LAST UPDATE:2023 08 01T00:17:16Z|External Id:SAMEA114192246|INSDC center alias:Institute of Molecular Genetics of the Czech Academy of Sciences|INSDC center name:Institute of Molecular Genetics of the Czech Academy of Sciences|INSDC first public:2023 08 01T00:17:16Z|INSDC last update:2023 08 01T00:17:16Z|INSDC status:public|Submitter Id:E MTAB 13196:fli1tp1 24hpf|age:24|broker name:ArrayExpress|cell type:endothelial cell|collection date:not collected|common name:zebrafish|developmental stage:24 hpf|genotype:fli1:DsRed tp1:eGFP|geographic location country and/or sea:not collected|immunophenotype:fli1:DsRed+ tp1:eGFP+|isolate:not applicable|organism part:embryo|sample name:E MTAB 13196:fli1tp1 24hpf|scientific name:Danio rerio|strain:fli1:DsRed tp1:eGFP | Illumina HiSeq 2500 paired end sequencing; Single cell RNA seq 10x Chromium of FACS sorted drl:H2B Dendra2 etv2:Kaede fli1:DsRed and tp1:eGFP transgenic zebrafish embryos | E MTAB 13196:fli1tp1 24hpf p | fli1tp1 24hpf p | Single cell RNA seq 10x Chromium of FACS sorted drl:H2B Dendra2 etv2:Kaede fli1:DsRed and tp1:eGFP transgenic zebrafish embryos | Dechorionated embryos were collected at defined stages dissociated by pipetting centrifugation at 300g for 5 minutes washed once with PBS and sorted by FACS. Total cell concentration and viability were determined using a TC20 Automated Cell Counter Bio Rad. Samples were then re suspended in 1x PBS with 0.05% BSA at 800 3000 cells per ml. Samples were loaded on the 10x Chromium system with a target of 4 000 cells. Chromium Single Cell 3สน GEM Library & Gel Bead Kit v2 or v3 was used for library preparation. Samples were loaded on the 10x Chromium system with a target of 4 000 cells. Chromium Single Cell 3สน GEM Library & Gel Bead Kit v2 or v3 was used for library preparation. | RNA-Seq | TRANSCRIPTOMIC SINGLE CELL | Oligo-dT | PAIRED | ILLUMINA | Illumina HiSeq 2500 | ERP149744 | Illumina HiSeq 2500 paired end sequencing; Single cell RNA seq 10x Chromium of FACS sorted drl:H2B Dendra2 etv2:Kaede fli1:DsRed and tp1:eGFP transgenic zebrafish embryos | ENA FIRST PUBLIC:2023 08 01|ENA LAST UPDATE:2023 08 01 | FliTp124hpf_S22_L001_R1_001.fastq.gz FliTp124hpf_S22_L001_R2_001.fastq.gz | fastq fastq | 864786636.0 | 6863386.0 | E MTAB 13196:FliTp124hpf S22 L001 | 0:28 1:98 | A:241767760;C:198370882;G:198049093;T:226508751;N:90150 | 28 | 98 | 241767760 | 198370882 | 198049093 | 226508751 | 90150 | ERX11157721 | ERS16172939 | ERA25628220 | Institute of Molecular Genetics of the Czech Academy of Sciences|European Nucleotide Archive | Institute of Molecular Genetics of the Czech Academy of Sciences|European Nucleotide Archive | 2 | 0.00375 | 0.89002 | 0.00114 | 0.0769 | 0.99237 | 0.84563 | 0.41796 | 0.48995 | 28 | 98 | T | B | sc-like readlen | illumina | hiseq_era | unknown | poly_a | unknown | sc | single_cell_droplet | 10x | Czech Republic | 2023-08-01 | Pharyngula | Embryo | Embryo Imprecise | All anatomical structures | ||||||||||||||
| 11757 | 11757 | ERR11758599 | ERX11157721 | ERS16172939 | ERP149744 | PRJEB64563 | Single cell RNA seq 10x Chromium of FACS sorted drl:H2B Dendra2 etv2:Kaede fli1:DsRed and tp1:eGFP transgenic zebrafish embryos | E-MTAB-13196 | Transcriptome Analysis | Development of the dorsal aorta is a key step in the establishment of the adult blood forming system since hematopoietic stem and progenitor cells HSPCs arise from ventral aortic endothelium in all vertebrate animals studied. Work in zebrafish has demonstrated that arterial and venous endothelial precursors arise from distinct subsets of lateral plate mesoderm. Here we profile the transcriptome of the earliest detectable endothelial cells ECs during zebrafish embryogenesis to demonstrate that tissue specific EC programs initiate much earlier than previously appreciated by the end of gastrulation. Classic studies in the chick embryo showed that paraxial mesoderm generates a subset of somite derived endothelial cells SDECs that incorporate into the dorsal aorta to replace HSPCs as they exit the aorta and enter circulation. We describe a conserved program in the zebrafish where a rare population of endothelial precursors delaminates from the dermomyotome to incorporate exclusively into the developing dorsal aorta. Whereas SDECs lack hematopoietic potential they act as a local niche to support the emergence of HSPCs from neighboring hemogenic endothelium. Thus at least three subsets of ECs contribute to the developing dorsal aorta: vascular ECs hemogenic ECs and SDECs. Taken together our findings indicate that the distinct spatial origins of endothelial precursors dictate different cellular potentials within the developing dorsal aorta. | ENA FIRST PUBLIC:2023 08 01|ENA LAST UPDATE:2023 08 01 | Protocols: Dechorionated embryos were collected at defined stages dissociated by pipetting centrifugation at 300g for 5 minutes washed once with PBS and sorted by FACS. Total cell concentration and viability were determined using a TC20 Automated Cell Counter Bio Rad. Samples were then re suspended in 1x PBS with 0.05% BSA at 800 3000 cells per ml. Samples were loaded on the 10x Chromium system with a target of 4 000 cells. Chromium Single Cell 3ʹ GEM Library & Gel Bead Kit v2 or v3 was used for library preparation. Samples were loaded on the 10x Chromium system with a target of 4 000 cells. Chromium Single Cell 3ʹ GEM Library & Gel Bead Kit v2 or v3 was used for library preparation. | fli1tp1 24hpf | SAMEA114192246 | Institute of Molecular Genetics of the Czech Academy of Sciences | ENA FIRST PUBLIC:2023 08 01T00:17:16Z|ENA LAST UPDATE:2023 08 01T00:17:16Z|External Id:SAMEA114192246|INSDC center alias:Institute of Molecular Genetics of the Czech Academy of Sciences|INSDC center name:Institute of Molecular Genetics of the Czech Academy of Sciences|INSDC first public:2023 08 01T00:17:16Z|INSDC last update:2023 08 01T00:17:16Z|INSDC status:public|Submitter Id:E MTAB 13196:fli1tp1 24hpf|age:24|broker name:ArrayExpress|cell type:endothelial cell|collection date:not collected|common name:zebrafish|developmental stage:24 hpf|genotype:fli1:DsRed tp1:eGFP|geographic location country and/or sea:not collected|immunophenotype:fli1:DsRed+ tp1:eGFP+|isolate:not applicable|organism part:embryo|sample name:E MTAB 13196:fli1tp1 24hpf|scientific name:Danio rerio|strain:fli1:DsRed tp1:eGFP | Illumina HiSeq 2500 paired end sequencing; Single cell RNA seq 10x Chromium of FACS sorted drl:H2B Dendra2 etv2:Kaede fli1:DsRed and tp1:eGFP transgenic zebrafish embryos | E MTAB 13196:fli1tp1 24hpf p | fli1tp1 24hpf p | Single cell RNA seq 10x Chromium of FACS sorted drl:H2B Dendra2 etv2:Kaede fli1:DsRed and tp1:eGFP transgenic zebrafish embryos | Dechorionated embryos were collected at defined stages dissociated by pipetting centrifugation at 300g for 5 minutes washed once with PBS and sorted by FACS. Total cell concentration and viability were determined using a TC20 Automated Cell Counter Bio Rad. Samples were then re suspended in 1x PBS with 0.05% BSA at 800 3000 cells per ml. Samples were loaded on the 10x Chromium system with a target of 4 000 cells. Chromium Single Cell 3สน GEM Library & Gel Bead Kit v2 or v3 was used for library preparation. Samples were loaded on the 10x Chromium system with a target of 4 000 cells. Chromium Single Cell 3สน GEM Library & Gel Bead Kit v2 or v3 was used for library preparation. | RNA-Seq | TRANSCRIPTOMIC SINGLE CELL | Oligo-dT | PAIRED | ILLUMINA | Illumina HiSeq 2500 | ERP149744 | Illumina HiSeq 2500 paired end sequencing; Single cell RNA seq 10x Chromium of FACS sorted drl:H2B Dendra2 etv2:Kaede fli1:DsRed and tp1:eGFP transgenic zebrafish embryos | ENA FIRST PUBLIC:2023 08 01|ENA LAST UPDATE:2023 08 01 | FliTp124hpf_S15_L005_R1_001.fastq.gz FliTp124hpf_S15_L005_R2_001.fastq.gz | fastq fastq | 1795799502.0 | 14252377.0 | E MTAB 13196:FliTp124hpf S15 L005 | 0:28 1:98 | A:500590460;C:413239086;G:410401125;T:471402890;N:165941 | 28 | 98 | 500590460 | 413239086 | 410401125 | 471402890 | 165941 | ERX11157721 | ERS16172939 | ERA25628220 | Institute of Molecular Genetics of the Czech Academy of Sciences|European Nucleotide Archive | Institute of Molecular Genetics of the Czech Academy of Sciences|European Nucleotide Archive | 2 | 0.00492 | 0.91242 | 0.0012 | 0.08044 | 0.99007 | 0.84668 | 0.42778 | 0.49291 | 28 | 98 | T | B | sc-like readlen | illumina | hiseq_era | unknown | poly_a | unknown | sc | single_cell_droplet | 10x | Czech Republic | 2023-08-01 | Pharyngula | Embryo | Embryo Imprecise | All anatomical structures | ||||||||||||||
| 11758 | 11758 | ERR11758601 | ERX11157721 | ERS16172939 | ERP149744 | PRJEB64563 | Single cell RNA seq 10x Chromium of FACS sorted drl:H2B Dendra2 etv2:Kaede fli1:DsRed and tp1:eGFP transgenic zebrafish embryos | E-MTAB-13196 | Transcriptome Analysis | Development of the dorsal aorta is a key step in the establishment of the adult blood forming system since hematopoietic stem and progenitor cells HSPCs arise from ventral aortic endothelium in all vertebrate animals studied. Work in zebrafish has demonstrated that arterial and venous endothelial precursors arise from distinct subsets of lateral plate mesoderm. Here we profile the transcriptome of the earliest detectable endothelial cells ECs during zebrafish embryogenesis to demonstrate that tissue specific EC programs initiate much earlier than previously appreciated by the end of gastrulation. Classic studies in the chick embryo showed that paraxial mesoderm generates a subset of somite derived endothelial cells SDECs that incorporate into the dorsal aorta to replace HSPCs as they exit the aorta and enter circulation. We describe a conserved program in the zebrafish where a rare population of endothelial precursors delaminates from the dermomyotome to incorporate exclusively into the developing dorsal aorta. Whereas SDECs lack hematopoietic potential they act as a local niche to support the emergence of HSPCs from neighboring hemogenic endothelium. Thus at least three subsets of ECs contribute to the developing dorsal aorta: vascular ECs hemogenic ECs and SDECs. Taken together our findings indicate that the distinct spatial origins of endothelial precursors dictate different cellular potentials within the developing dorsal aorta. | ENA FIRST PUBLIC:2023 08 01|ENA LAST UPDATE:2023 08 01 | Protocols: Dechorionated embryos were collected at defined stages dissociated by pipetting centrifugation at 300g for 5 minutes washed once with PBS and sorted by FACS. Total cell concentration and viability were determined using a TC20 Automated Cell Counter Bio Rad. Samples were then re suspended in 1x PBS with 0.05% BSA at 800 3000 cells per ml. Samples were loaded on the 10x Chromium system with a target of 4 000 cells. Chromium Single Cell 3ʹ GEM Library & Gel Bead Kit v2 or v3 was used for library preparation. Samples were loaded on the 10x Chromium system with a target of 4 000 cells. Chromium Single Cell 3ʹ GEM Library & Gel Bead Kit v2 or v3 was used for library preparation. | fli1tp1 24hpf | SAMEA114192246 | Institute of Molecular Genetics of the Czech Academy of Sciences | ENA FIRST PUBLIC:2023 08 01T00:17:16Z|ENA LAST UPDATE:2023 08 01T00:17:16Z|External Id:SAMEA114192246|INSDC center alias:Institute of Molecular Genetics of the Czech Academy of Sciences|INSDC center name:Institute of Molecular Genetics of the Czech Academy of Sciences|INSDC first public:2023 08 01T00:17:16Z|INSDC last update:2023 08 01T00:17:16Z|INSDC status:public|Submitter Id:E MTAB 13196:fli1tp1 24hpf|age:24|broker name:ArrayExpress|cell type:endothelial cell|collection date:not collected|common name:zebrafish|developmental stage:24 hpf|genotype:fli1:DsRed tp1:eGFP|geographic location country and/or sea:not collected|immunophenotype:fli1:DsRed+ tp1:eGFP+|isolate:not applicable|organism part:embryo|sample name:E MTAB 13196:fli1tp1 24hpf|scientific name:Danio rerio|strain:fli1:DsRed tp1:eGFP | Illumina HiSeq 2500 paired end sequencing; Single cell RNA seq 10x Chromium of FACS sorted drl:H2B Dendra2 etv2:Kaede fli1:DsRed and tp1:eGFP transgenic zebrafish embryos | E MTAB 13196:fli1tp1 24hpf p | fli1tp1 24hpf p | Single cell RNA seq 10x Chromium of FACS sorted drl:H2B Dendra2 etv2:Kaede fli1:DsRed and tp1:eGFP transgenic zebrafish embryos | Dechorionated embryos were collected at defined stages dissociated by pipetting centrifugation at 300g for 5 minutes washed once with PBS and sorted by FACS. Total cell concentration and viability were determined using a TC20 Automated Cell Counter Bio Rad. Samples were then re suspended in 1x PBS with 0.05% BSA at 800 3000 cells per ml. Samples were loaded on the 10x Chromium system with a target of 4 000 cells. Chromium Single Cell 3สน GEM Library & Gel Bead Kit v2 or v3 was used for library preparation. Samples were loaded on the 10x Chromium system with a target of 4 000 cells. Chromium Single Cell 3สน GEM Library & Gel Bead Kit v2 or v3 was used for library preparation. | RNA-Seq | TRANSCRIPTOMIC SINGLE CELL | Oligo-dT | PAIRED | ILLUMINA | Illumina HiSeq 2500 | ERP149744 | Illumina HiSeq 2500 paired end sequencing; Single cell RNA seq 10x Chromium of FACS sorted drl:H2B Dendra2 etv2:Kaede fli1:DsRed and tp1:eGFP transgenic zebrafish embryos | ENA FIRST PUBLIC:2023 08 01|ENA LAST UPDATE:2023 08 01 | FliTp124hpf_S26_L001_R1_001.fastq.gz FliTp124hpf_S26_L001_R2_001.fastq.gz | fastq fastq | 266141358.0 | 2112233.0 | E MTAB 13196:FliTp124hpf S26 L001 | 0:28 1:98 | A:76996100;C:59592346;G:58963461;T:70457732;N:131719 | 28 | 98 | 76996100 | 59592346 | 58963461 | 70457732 | 131719 | ERX11157721 | ERS16172939 | ERA25628220 | Institute of Molecular Genetics of the Czech Academy of Sciences|European Nucleotide Archive | Institute of Molecular Genetics of the Czech Academy of Sciences|European Nucleotide Archive | 2 | 0.00106 | 0.88422 | 0.00046 | 0.09358 | 0.99782 | 0.89449 | 0.47826 | 0.48948 | 28 | 98 | T | B | sc-like readlen | illumina | hiseq_era | unknown | poly_a | unknown | sc | single_cell_droplet | 10x | Czech Republic | 2023-08-01 | Pharyngula | Embryo | Embryo Imprecise | All anatomical structures | ||||||||||||||
| 11759 | 11759 | ERR11758630 | ERX11157721 | ERS16172939 | ERP149744 | PRJEB64563 | Single cell RNA seq 10x Chromium of FACS sorted drl:H2B Dendra2 etv2:Kaede fli1:DsRed and tp1:eGFP transgenic zebrafish embryos | E-MTAB-13196 | Transcriptome Analysis | Development of the dorsal aorta is a key step in the establishment of the adult blood forming system since hematopoietic stem and progenitor cells HSPCs arise from ventral aortic endothelium in all vertebrate animals studied. Work in zebrafish has demonstrated that arterial and venous endothelial precursors arise from distinct subsets of lateral plate mesoderm. Here we profile the transcriptome of the earliest detectable endothelial cells ECs during zebrafish embryogenesis to demonstrate that tissue specific EC programs initiate much earlier than previously appreciated by the end of gastrulation. Classic studies in the chick embryo showed that paraxial mesoderm generates a subset of somite derived endothelial cells SDECs that incorporate into the dorsal aorta to replace HSPCs as they exit the aorta and enter circulation. We describe a conserved program in the zebrafish where a rare population of endothelial precursors delaminates from the dermomyotome to incorporate exclusively into the developing dorsal aorta. Whereas SDECs lack hematopoietic potential they act as a local niche to support the emergence of HSPCs from neighboring hemogenic endothelium. Thus at least three subsets of ECs contribute to the developing dorsal aorta: vascular ECs hemogenic ECs and SDECs. Taken together our findings indicate that the distinct spatial origins of endothelial precursors dictate different cellular potentials within the developing dorsal aorta. | ENA FIRST PUBLIC:2023 08 01|ENA LAST UPDATE:2023 08 01 | Protocols: Dechorionated embryos were collected at defined stages dissociated by pipetting centrifugation at 300g for 5 minutes washed once with PBS and sorted by FACS. Total cell concentration and viability were determined using a TC20 Automated Cell Counter Bio Rad. Samples were then re suspended in 1x PBS with 0.05% BSA at 800 3000 cells per ml. Samples were loaded on the 10x Chromium system with a target of 4 000 cells. Chromium Single Cell 3ʹ GEM Library & Gel Bead Kit v2 or v3 was used for library preparation. Samples were loaded on the 10x Chromium system with a target of 4 000 cells. Chromium Single Cell 3ʹ GEM Library & Gel Bead Kit v2 or v3 was used for library preparation. | fli1tp1 24hpf | SAMEA114192246 | Institute of Molecular Genetics of the Czech Academy of Sciences | ENA FIRST PUBLIC:2023 08 01T00:17:16Z|ENA LAST UPDATE:2023 08 01T00:17:16Z|External Id:SAMEA114192246|INSDC center alias:Institute of Molecular Genetics of the Czech Academy of Sciences|INSDC center name:Institute of Molecular Genetics of the Czech Academy of Sciences|INSDC first public:2023 08 01T00:17:16Z|INSDC last update:2023 08 01T00:17:16Z|INSDC status:public|Submitter Id:E MTAB 13196:fli1tp1 24hpf|age:24|broker name:ArrayExpress|cell type:endothelial cell|collection date:not collected|common name:zebrafish|developmental stage:24 hpf|genotype:fli1:DsRed tp1:eGFP|geographic location country and/or sea:not collected|immunophenotype:fli1:DsRed+ tp1:eGFP+|isolate:not applicable|organism part:embryo|sample name:E MTAB 13196:fli1tp1 24hpf|scientific name:Danio rerio|strain:fli1:DsRed tp1:eGFP | Illumina HiSeq 2500 paired end sequencing; Single cell RNA seq 10x Chromium of FACS sorted drl:H2B Dendra2 etv2:Kaede fli1:DsRed and tp1:eGFP transgenic zebrafish embryos | E MTAB 13196:fli1tp1 24hpf p | fli1tp1 24hpf p | Single cell RNA seq 10x Chromium of FACS sorted drl:H2B Dendra2 etv2:Kaede fli1:DsRed and tp1:eGFP transgenic zebrafish embryos | Dechorionated embryos were collected at defined stages dissociated by pipetting centrifugation at 300g for 5 minutes washed once with PBS and sorted by FACS. Total cell concentration and viability were determined using a TC20 Automated Cell Counter Bio Rad. Samples were then re suspended in 1x PBS with 0.05% BSA at 800 3000 cells per ml. Samples were loaded on the 10x Chromium system with a target of 4 000 cells. Chromium Single Cell 3สน GEM Library & Gel Bead Kit v2 or v3 was used for library preparation. Samples were loaded on the 10x Chromium system with a target of 4 000 cells. Chromium Single Cell 3สน GEM Library & Gel Bead Kit v2 or v3 was used for library preparation. | RNA-Seq | TRANSCRIPTOMIC SINGLE CELL | Oligo-dT | PAIRED | ILLUMINA | Illumina HiSeq 2500 | ERP149744 | Illumina HiSeq 2500 paired end sequencing; Single cell RNA seq 10x Chromium of FACS sorted drl:H2B Dendra2 etv2:Kaede fli1:DsRed and tp1:eGFP transgenic zebrafish embryos | ENA FIRST PUBLIC:2023 08 01|ENA LAST UPDATE:2023 08 01 | FliTp124hpf_S14_L005_R1_001.fastq.gz FliTp124hpf_S14_L005_R2_001.fastq.gz | fastq fastq | 1812847428.0 | 14387678.0 | E MTAB 13196:FliTp124hpf S14 L005 | 0:28 1:98 | A:502956890;C:417865079;G:416595454;T:475266679;N:163326 | 28 | 98 | 502956890 | 417865079 | 416595454 | 475266679 | 163326 | ERX11157721 | ERS16172939 | ERA25628220 | Institute of Molecular Genetics of the Czech Academy of Sciences|European Nucleotide Archive | Institute of Molecular Genetics of the Czech Academy of Sciences|European Nucleotide Archive | 2 | 0.00483 | 0.91317 | 0.00123 | 0.07918 | 0.99036 | 0.84143 | 0.40704 | 0.4819 | 28 | 98 | T | B | sc-like readlen | illumina | hiseq_era | unknown | poly_a | unknown | sc | single_cell_droplet | 10x | Czech Republic | 2023-08-01 | Pharyngula | Embryo | Embryo Imprecise | All anatomical structures | ||||||||||||||
| 11760 | 11760 | ERR11758603 | ERX11157721 | ERS16172939 | ERP149744 | PRJEB64563 | Single cell RNA seq 10x Chromium of FACS sorted drl:H2B Dendra2 etv2:Kaede fli1:DsRed and tp1:eGFP transgenic zebrafish embryos | E-MTAB-13196 | Transcriptome Analysis | Development of the dorsal aorta is a key step in the establishment of the adult blood forming system since hematopoietic stem and progenitor cells HSPCs arise from ventral aortic endothelium in all vertebrate animals studied. Work in zebrafish has demonstrated that arterial and venous endothelial precursors arise from distinct subsets of lateral plate mesoderm. Here we profile the transcriptome of the earliest detectable endothelial cells ECs during zebrafish embryogenesis to demonstrate that tissue specific EC programs initiate much earlier than previously appreciated by the end of gastrulation. Classic studies in the chick embryo showed that paraxial mesoderm generates a subset of somite derived endothelial cells SDECs that incorporate into the dorsal aorta to replace HSPCs as they exit the aorta and enter circulation. We describe a conserved program in the zebrafish where a rare population of endothelial precursors delaminates from the dermomyotome to incorporate exclusively into the developing dorsal aorta. Whereas SDECs lack hematopoietic potential they act as a local niche to support the emergence of HSPCs from neighboring hemogenic endothelium. Thus at least three subsets of ECs contribute to the developing dorsal aorta: vascular ECs hemogenic ECs and SDECs. Taken together our findings indicate that the distinct spatial origins of endothelial precursors dictate different cellular potentials within the developing dorsal aorta. | ENA FIRST PUBLIC:2023 08 01|ENA LAST UPDATE:2023 08 01 | Protocols: Dechorionated embryos were collected at defined stages dissociated by pipetting centrifugation at 300g for 5 minutes washed once with PBS and sorted by FACS. Total cell concentration and viability were determined using a TC20 Automated Cell Counter Bio Rad. Samples were then re suspended in 1x PBS with 0.05% BSA at 800 3000 cells per ml. Samples were loaded on the 10x Chromium system with a target of 4 000 cells. Chromium Single Cell 3ʹ GEM Library & Gel Bead Kit v2 or v3 was used for library preparation. Samples were loaded on the 10x Chromium system with a target of 4 000 cells. Chromium Single Cell 3ʹ GEM Library & Gel Bead Kit v2 or v3 was used for library preparation. | fli1tp1 24hpf | SAMEA114192246 | Institute of Molecular Genetics of the Czech Academy of Sciences | ENA FIRST PUBLIC:2023 08 01T00:17:16Z|ENA LAST UPDATE:2023 08 01T00:17:16Z|External Id:SAMEA114192246|INSDC center alias:Institute of Molecular Genetics of the Czech Academy of Sciences|INSDC center name:Institute of Molecular Genetics of the Czech Academy of Sciences|INSDC first public:2023 08 01T00:17:16Z|INSDC last update:2023 08 01T00:17:16Z|INSDC status:public|Submitter Id:E MTAB 13196:fli1tp1 24hpf|age:24|broker name:ArrayExpress|cell type:endothelial cell|collection date:not collected|common name:zebrafish|developmental stage:24 hpf|genotype:fli1:DsRed tp1:eGFP|geographic location country and/or sea:not collected|immunophenotype:fli1:DsRed+ tp1:eGFP+|isolate:not applicable|organism part:embryo|sample name:E MTAB 13196:fli1tp1 24hpf|scientific name:Danio rerio|strain:fli1:DsRed tp1:eGFP | Illumina HiSeq 2500 paired end sequencing; Single cell RNA seq 10x Chromium of FACS sorted drl:H2B Dendra2 etv2:Kaede fli1:DsRed and tp1:eGFP transgenic zebrafish embryos | E MTAB 13196:fli1tp1 24hpf p | fli1tp1 24hpf p | Single cell RNA seq 10x Chromium of FACS sorted drl:H2B Dendra2 etv2:Kaede fli1:DsRed and tp1:eGFP transgenic zebrafish embryos | Dechorionated embryos were collected at defined stages dissociated by pipetting centrifugation at 300g for 5 minutes washed once with PBS and sorted by FACS. Total cell concentration and viability were determined using a TC20 Automated Cell Counter Bio Rad. Samples were then re suspended in 1x PBS with 0.05% BSA at 800 3000 cells per ml. Samples were loaded on the 10x Chromium system with a target of 4 000 cells. Chromium Single Cell 3สน GEM Library & Gel Bead Kit v2 or v3 was used for library preparation. Samples were loaded on the 10x Chromium system with a target of 4 000 cells. Chromium Single Cell 3สน GEM Library & Gel Bead Kit v2 or v3 was used for library preparation. | RNA-Seq | TRANSCRIPTOMIC SINGLE CELL | Oligo-dT | PAIRED | ILLUMINA | Illumina HiSeq 2500 | ERP149744 | Illumina HiSeq 2500 paired end sequencing; Single cell RNA seq 10x Chromium of FACS sorted drl:H2B Dendra2 etv2:Kaede fli1:DsRed and tp1:eGFP transgenic zebrafish embryos | ENA FIRST PUBLIC:2023 08 01|ENA LAST UPDATE:2023 08 01 | FliTp124hpf_S13_L005_R1_001.fastq.gz FliTp124hpf_S13_L005_R2_001.fastq.gz | fastq fastq | 1544281830.0 | 12256205.0 | E MTAB 13196:FliTp124hpf S13 L005 | 0:28 1:98 | A:428008915;C:356252081;G:354333187;T:405545992;N:141655 | 28 | 98 | 428008915 | 356252081 | 354333187 | 405545992 | 141655 | ERX11157721 | ERS16172939 | ERA25628220 | Institute of Molecular Genetics of the Czech Academy of Sciences|European Nucleotide Archive | Institute of Molecular Genetics of the Czech Academy of Sciences|European Nucleotide Archive | 2 | 0.00507 | 0.91025 | 0.00138 | 0.07858 | 0.98987 | 0.84295 | 0.41793 | 0.4891 | 28 | 98 | T | B | sc-like readlen | illumina | hiseq_era | unknown | poly_a | unknown | sc | single_cell_droplet | 10x | Czech Republic | 2023-08-01 | Pharyngula | Embryo | Embryo Imprecise | All anatomical structures | ||||||||||||||
| 11761 | 11761 | ERR11758613 | ERX11157721 | ERS16172939 | ERP149744 | PRJEB64563 | Single cell RNA seq 10x Chromium of FACS sorted drl:H2B Dendra2 etv2:Kaede fli1:DsRed and tp1:eGFP transgenic zebrafish embryos | E-MTAB-13196 | Transcriptome Analysis | Development of the dorsal aorta is a key step in the establishment of the adult blood forming system since hematopoietic stem and progenitor cells HSPCs arise from ventral aortic endothelium in all vertebrate animals studied. Work in zebrafish has demonstrated that arterial and venous endothelial precursors arise from distinct subsets of lateral plate mesoderm. Here we profile the transcriptome of the earliest detectable endothelial cells ECs during zebrafish embryogenesis to demonstrate that tissue specific EC programs initiate much earlier than previously appreciated by the end of gastrulation. Classic studies in the chick embryo showed that paraxial mesoderm generates a subset of somite derived endothelial cells SDECs that incorporate into the dorsal aorta to replace HSPCs as they exit the aorta and enter circulation. We describe a conserved program in the zebrafish where a rare population of endothelial precursors delaminates from the dermomyotome to incorporate exclusively into the developing dorsal aorta. Whereas SDECs lack hematopoietic potential they act as a local niche to support the emergence of HSPCs from neighboring hemogenic endothelium. Thus at least three subsets of ECs contribute to the developing dorsal aorta: vascular ECs hemogenic ECs and SDECs. Taken together our findings indicate that the distinct spatial origins of endothelial precursors dictate different cellular potentials within the developing dorsal aorta. | ENA FIRST PUBLIC:2023 08 01|ENA LAST UPDATE:2023 08 01 | Protocols: Dechorionated embryos were collected at defined stages dissociated by pipetting centrifugation at 300g for 5 minutes washed once with PBS and sorted by FACS. Total cell concentration and viability were determined using a TC20 Automated Cell Counter Bio Rad. Samples were then re suspended in 1x PBS with 0.05% BSA at 800 3000 cells per ml. Samples were loaded on the 10x Chromium system with a target of 4 000 cells. Chromium Single Cell 3ʹ GEM Library & Gel Bead Kit v2 or v3 was used for library preparation. Samples were loaded on the 10x Chromium system with a target of 4 000 cells. Chromium Single Cell 3ʹ GEM Library & Gel Bead Kit v2 or v3 was used for library preparation. | fli1tp1 24hpf | SAMEA114192246 | Institute of Molecular Genetics of the Czech Academy of Sciences | ENA FIRST PUBLIC:2023 08 01T00:17:16Z|ENA LAST UPDATE:2023 08 01T00:17:16Z|External Id:SAMEA114192246|INSDC center alias:Institute of Molecular Genetics of the Czech Academy of Sciences|INSDC center name:Institute of Molecular Genetics of the Czech Academy of Sciences|INSDC first public:2023 08 01T00:17:16Z|INSDC last update:2023 08 01T00:17:16Z|INSDC status:public|Submitter Id:E MTAB 13196:fli1tp1 24hpf|age:24|broker name:ArrayExpress|cell type:endothelial cell|collection date:not collected|common name:zebrafish|developmental stage:24 hpf|genotype:fli1:DsRed tp1:eGFP|geographic location country and/or sea:not collected|immunophenotype:fli1:DsRed+ tp1:eGFP+|isolate:not applicable|organism part:embryo|sample name:E MTAB 13196:fli1tp1 24hpf|scientific name:Danio rerio|strain:fli1:DsRed tp1:eGFP | Illumina HiSeq 2500 paired end sequencing; Single cell RNA seq 10x Chromium of FACS sorted drl:H2B Dendra2 etv2:Kaede fli1:DsRed and tp1:eGFP transgenic zebrafish embryos | E MTAB 13196:fli1tp1 24hpf p | fli1tp1 24hpf p | Single cell RNA seq 10x Chromium of FACS sorted drl:H2B Dendra2 etv2:Kaede fli1:DsRed and tp1:eGFP transgenic zebrafish embryos | Dechorionated embryos were collected at defined stages dissociated by pipetting centrifugation at 300g for 5 minutes washed once with PBS and sorted by FACS. Total cell concentration and viability were determined using a TC20 Automated Cell Counter Bio Rad. Samples were then re suspended in 1x PBS with 0.05% BSA at 800 3000 cells per ml. Samples were loaded on the 10x Chromium system with a target of 4 000 cells. Chromium Single Cell 3สน GEM Library & Gel Bead Kit v2 or v3 was used for library preparation. Samples were loaded on the 10x Chromium system with a target of 4 000 cells. Chromium Single Cell 3สน GEM Library & Gel Bead Kit v2 or v3 was used for library preparation. | RNA-Seq | TRANSCRIPTOMIC SINGLE CELL | Oligo-dT | PAIRED | ILLUMINA | Illumina HiSeq 2500 | ERP149744 | Illumina HiSeq 2500 paired end sequencing; Single cell RNA seq 10x Chromium of FACS sorted drl:H2B Dendra2 etv2:Kaede fli1:DsRed and tp1:eGFP transgenic zebrafish embryos | ENA FIRST PUBLIC:2023 08 01|ENA LAST UPDATE:2023 08 01 | FliTp124hpf_S21_L001_R1_001.fastq.gz FliTp124hpf_S21_L001_R2_001.fastq.gz | fastq fastq | 761441436.0 | 6043186.0 | E MTAB 13196:FliTp124hpf S21 L001 | 0:28 1:98 | A:212876877;C:174700212;G:174244892;T:199537401;N:82054 | 28 | 98 | 212876877 | 174700212 | 174244892 | 199537401 | 82054 | ERX11157721 | ERS16172939 | ERA25628220 | Institute of Molecular Genetics of the Czech Academy of Sciences|European Nucleotide Archive | Institute of Molecular Genetics of the Czech Academy of Sciences|European Nucleotide Archive | 2 | 0.00334 | 0.88899 | 0.00106 | 0.07762 | 0.99322 | 0.84476 | 0.42792 | 0.48783 | 28 | 98 | T | B | sc-like readlen | illumina | hiseq_era | unknown | poly_a | unknown | sc | single_cell_droplet | 10x | Czech Republic | 2023-08-01 | Pharyngula | Embryo | Embryo Imprecise | All anatomical structures | ||||||||||||||
| 11762 | 11762 | ERR11758645 | ERX11157721 | ERS16172939 | ERP149744 | PRJEB64563 | Single cell RNA seq 10x Chromium of FACS sorted drl:H2B Dendra2 etv2:Kaede fli1:DsRed and tp1:eGFP transgenic zebrafish embryos | E-MTAB-13196 | Transcriptome Analysis | Development of the dorsal aorta is a key step in the establishment of the adult blood forming system since hematopoietic stem and progenitor cells HSPCs arise from ventral aortic endothelium in all vertebrate animals studied. Work in zebrafish has demonstrated that arterial and venous endothelial precursors arise from distinct subsets of lateral plate mesoderm. Here we profile the transcriptome of the earliest detectable endothelial cells ECs during zebrafish embryogenesis to demonstrate that tissue specific EC programs initiate much earlier than previously appreciated by the end of gastrulation. Classic studies in the chick embryo showed that paraxial mesoderm generates a subset of somite derived endothelial cells SDECs that incorporate into the dorsal aorta to replace HSPCs as they exit the aorta and enter circulation. We describe a conserved program in the zebrafish where a rare population of endothelial precursors delaminates from the dermomyotome to incorporate exclusively into the developing dorsal aorta. Whereas SDECs lack hematopoietic potential they act as a local niche to support the emergence of HSPCs from neighboring hemogenic endothelium. Thus at least three subsets of ECs contribute to the developing dorsal aorta: vascular ECs hemogenic ECs and SDECs. Taken together our findings indicate that the distinct spatial origins of endothelial precursors dictate different cellular potentials within the developing dorsal aorta. | ENA FIRST PUBLIC:2023 08 01|ENA LAST UPDATE:2023 08 01 | Protocols: Dechorionated embryos were collected at defined stages dissociated by pipetting centrifugation at 300g for 5 minutes washed once with PBS and sorted by FACS. Total cell concentration and viability were determined using a TC20 Automated Cell Counter Bio Rad. Samples were then re suspended in 1x PBS with 0.05% BSA at 800 3000 cells per ml. Samples were loaded on the 10x Chromium system with a target of 4 000 cells. Chromium Single Cell 3ʹ GEM Library & Gel Bead Kit v2 or v3 was used for library preparation. Samples were loaded on the 10x Chromium system with a target of 4 000 cells. Chromium Single Cell 3ʹ GEM Library & Gel Bead Kit v2 or v3 was used for library preparation. | fli1tp1 24hpf | SAMEA114192246 | Institute of Molecular Genetics of the Czech Academy of Sciences | ENA FIRST PUBLIC:2023 08 01T00:17:16Z|ENA LAST UPDATE:2023 08 01T00:17:16Z|External Id:SAMEA114192246|INSDC center alias:Institute of Molecular Genetics of the Czech Academy of Sciences|INSDC center name:Institute of Molecular Genetics of the Czech Academy of Sciences|INSDC first public:2023 08 01T00:17:16Z|INSDC last update:2023 08 01T00:17:16Z|INSDC status:public|Submitter Id:E MTAB 13196:fli1tp1 24hpf|age:24|broker name:ArrayExpress|cell type:endothelial cell|collection date:not collected|common name:zebrafish|developmental stage:24 hpf|genotype:fli1:DsRed tp1:eGFP|geographic location country and/or sea:not collected|immunophenotype:fli1:DsRed+ tp1:eGFP+|isolate:not applicable|organism part:embryo|sample name:E MTAB 13196:fli1tp1 24hpf|scientific name:Danio rerio|strain:fli1:DsRed tp1:eGFP | Illumina HiSeq 2500 paired end sequencing; Single cell RNA seq 10x Chromium of FACS sorted drl:H2B Dendra2 etv2:Kaede fli1:DsRed and tp1:eGFP transgenic zebrafish embryos | E MTAB 13196:fli1tp1 24hpf p | fli1tp1 24hpf p | Single cell RNA seq 10x Chromium of FACS sorted drl:H2B Dendra2 etv2:Kaede fli1:DsRed and tp1:eGFP transgenic zebrafish embryos | Dechorionated embryos were collected at defined stages dissociated by pipetting centrifugation at 300g for 5 minutes washed once with PBS and sorted by FACS. Total cell concentration and viability were determined using a TC20 Automated Cell Counter Bio Rad. Samples were then re suspended in 1x PBS with 0.05% BSA at 800 3000 cells per ml. Samples were loaded on the 10x Chromium system with a target of 4 000 cells. Chromium Single Cell 3สน GEM Library & Gel Bead Kit v2 or v3 was used for library preparation. Samples were loaded on the 10x Chromium system with a target of 4 000 cells. Chromium Single Cell 3สน GEM Library & Gel Bead Kit v2 or v3 was used for library preparation. | RNA-Seq | TRANSCRIPTOMIC SINGLE CELL | Oligo-dT | PAIRED | ILLUMINA | Illumina HiSeq 2500 | ERP149744 | Illumina HiSeq 2500 paired end sequencing; Single cell RNA seq 10x Chromium of FACS sorted drl:H2B Dendra2 etv2:Kaede fli1:DsRed and tp1:eGFP transgenic zebrafish embryos | ENA FIRST PUBLIC:2023 08 01|ENA LAST UPDATE:2023 08 01 | FliTp124hpf_S23_L001_R1_001.fastq.gz FliTp124hpf_S23_L001_R2_001.fastq.gz | fastq fastq | 873938772.0 | 6936022.0 | E MTAB 13196:FliTp124hpf S23 L001 | 0:28 1:98 | A:245075115;C:199879929;G:199312177;T:229576663;N:94888 | 28 | 98 | 245075115 | 199879929 | 199312177 | 229576663 | 94888 | ERX11157721 | ERS16172939 | ERA25628220 | Institute of Molecular Genetics of the Czech Academy of Sciences|European Nucleotide Archive | Institute of Molecular Genetics of the Czech Academy of Sciences|European Nucleotide Archive | 2 | 0.00361 | 0.88671 | 0.00104 | 0.07855 | 0.99255 | 0.84723 | 0.41141 | 0.4885 | 28 | 98 | T | B | sc-like readlen | illumina | hiseq_era | unknown | poly_a | unknown | sc | single_cell_droplet | 10x | Czech Republic | 2023-08-01 | Pharyngula | Embryo | Embryo Imprecise | All anatomical structures | ||||||||||||||
| 11763 | 11763 | ERR11758598 | ERX11157721 | ERS16172939 | ERP149744 | PRJEB64563 | Single cell RNA seq 10x Chromium of FACS sorted drl:H2B Dendra2 etv2:Kaede fli1:DsRed and tp1:eGFP transgenic zebrafish embryos | E-MTAB-13196 | Transcriptome Analysis | Development of the dorsal aorta is a key step in the establishment of the adult blood forming system since hematopoietic stem and progenitor cells HSPCs arise from ventral aortic endothelium in all vertebrate animals studied. Work in zebrafish has demonstrated that arterial and venous endothelial precursors arise from distinct subsets of lateral plate mesoderm. Here we profile the transcriptome of the earliest detectable endothelial cells ECs during zebrafish embryogenesis to demonstrate that tissue specific EC programs initiate much earlier than previously appreciated by the end of gastrulation. Classic studies in the chick embryo showed that paraxial mesoderm generates a subset of somite derived endothelial cells SDECs that incorporate into the dorsal aorta to replace HSPCs as they exit the aorta and enter circulation. We describe a conserved program in the zebrafish where a rare population of endothelial precursors delaminates from the dermomyotome to incorporate exclusively into the developing dorsal aorta. Whereas SDECs lack hematopoietic potential they act as a local niche to support the emergence of HSPCs from neighboring hemogenic endothelium. Thus at least three subsets of ECs contribute to the developing dorsal aorta: vascular ECs hemogenic ECs and SDECs. Taken together our findings indicate that the distinct spatial origins of endothelial precursors dictate different cellular potentials within the developing dorsal aorta. | ENA FIRST PUBLIC:2023 08 01|ENA LAST UPDATE:2023 08 01 | Protocols: Dechorionated embryos were collected at defined stages dissociated by pipetting centrifugation at 300g for 5 minutes washed once with PBS and sorted by FACS. Total cell concentration and viability were determined using a TC20 Automated Cell Counter Bio Rad. Samples were then re suspended in 1x PBS with 0.05% BSA at 800 3000 cells per ml. Samples were loaded on the 10x Chromium system with a target of 4 000 cells. Chromium Single Cell 3ʹ GEM Library & Gel Bead Kit v2 or v3 was used for library preparation. Samples were loaded on the 10x Chromium system with a target of 4 000 cells. Chromium Single Cell 3ʹ GEM Library & Gel Bead Kit v2 or v3 was used for library preparation. | fli1tp1 24hpf | SAMEA114192246 | Institute of Molecular Genetics of the Czech Academy of Sciences | ENA FIRST PUBLIC:2023 08 01T00:17:16Z|ENA LAST UPDATE:2023 08 01T00:17:16Z|External Id:SAMEA114192246|INSDC center alias:Institute of Molecular Genetics of the Czech Academy of Sciences|INSDC center name:Institute of Molecular Genetics of the Czech Academy of Sciences|INSDC first public:2023 08 01T00:17:16Z|INSDC last update:2023 08 01T00:17:16Z|INSDC status:public|Submitter Id:E MTAB 13196:fli1tp1 24hpf|age:24|broker name:ArrayExpress|cell type:endothelial cell|collection date:not collected|common name:zebrafish|developmental stage:24 hpf|genotype:fli1:DsRed tp1:eGFP|geographic location country and/or sea:not collected|immunophenotype:fli1:DsRed+ tp1:eGFP+|isolate:not applicable|organism part:embryo|sample name:E MTAB 13196:fli1tp1 24hpf|scientific name:Danio rerio|strain:fli1:DsRed tp1:eGFP | Illumina HiSeq 2500 paired end sequencing; Single cell RNA seq 10x Chromium of FACS sorted drl:H2B Dendra2 etv2:Kaede fli1:DsRed and tp1:eGFP transgenic zebrafish embryos | E MTAB 13196:fli1tp1 24hpf p | fli1tp1 24hpf p | Single cell RNA seq 10x Chromium of FACS sorted drl:H2B Dendra2 etv2:Kaede fli1:DsRed and tp1:eGFP transgenic zebrafish embryos | Dechorionated embryos were collected at defined stages dissociated by pipetting centrifugation at 300g for 5 minutes washed once with PBS and sorted by FACS. Total cell concentration and viability were determined using a TC20 Automated Cell Counter Bio Rad. Samples were then re suspended in 1x PBS with 0.05% BSA at 800 3000 cells per ml. Samples were loaded on the 10x Chromium system with a target of 4 000 cells. Chromium Single Cell 3สน GEM Library & Gel Bead Kit v2 or v3 was used for library preparation. Samples were loaded on the 10x Chromium system with a target of 4 000 cells. Chromium Single Cell 3สน GEM Library & Gel Bead Kit v2 or v3 was used for library preparation. | RNA-Seq | TRANSCRIPTOMIC SINGLE CELL | Oligo-dT | PAIRED | ILLUMINA | Illumina HiSeq 2500 | ERP149744 | Illumina HiSeq 2500 paired end sequencing; Single cell RNA seq 10x Chromium of FACS sorted drl:H2B Dendra2 etv2:Kaede fli1:DsRed and tp1:eGFP transgenic zebrafish embryos | ENA FIRST PUBLIC:2023 08 01|ENA LAST UPDATE:2023 08 01 | FliTp124hpf_S28_L001_R1_001.fastq.gz FliTp124hpf_S28_L001_R2_001.fastq.gz | fastq fastq | 164465532.0 | 1305282.0 | E MTAB 13196:FliTp124hpf S28 L001 | 0:28 1:98 | A:47319366;C:37063279;G:36394680;T:43602586;N:85621 | 28 | 98 | 47319366 | 37063279 | 36394680 | 43602586 | 85621 | ERX11157721 | ERS16172939 | ERA25628220 | Institute of Molecular Genetics of the Czech Academy of Sciences|European Nucleotide Archive | Institute of Molecular Genetics of the Czech Academy of Sciences|European Nucleotide Archive | 2 | 0.00117 | 0.88222 | 0.00058 | 0.09358 | 0.99799 | 0.89217 | 0.54867 | 0.48545 | 28 | 98 | T | B | sc-like readlen | illumina | hiseq_era | unknown | poly_a | unknown | sc | single_cell_droplet | 10x | Czech Republic | 2023-08-01 | Pharyngula | Embryo | Embryo Imprecise | All anatomical structures | ||||||||||||||
| 11764 | 11764 | ERR11758593 | ERX11157721 | ERS16172939 | ERP149744 | PRJEB64563 | Single cell RNA seq 10x Chromium of FACS sorted drl:H2B Dendra2 etv2:Kaede fli1:DsRed and tp1:eGFP transgenic zebrafish embryos | E-MTAB-13196 | Transcriptome Analysis | Development of the dorsal aorta is a key step in the establishment of the adult blood forming system since hematopoietic stem and progenitor cells HSPCs arise from ventral aortic endothelium in all vertebrate animals studied. Work in zebrafish has demonstrated that arterial and venous endothelial precursors arise from distinct subsets of lateral plate mesoderm. Here we profile the transcriptome of the earliest detectable endothelial cells ECs during zebrafish embryogenesis to demonstrate that tissue specific EC programs initiate much earlier than previously appreciated by the end of gastrulation. Classic studies in the chick embryo showed that paraxial mesoderm generates a subset of somite derived endothelial cells SDECs that incorporate into the dorsal aorta to replace HSPCs as they exit the aorta and enter circulation. We describe a conserved program in the zebrafish where a rare population of endothelial precursors delaminates from the dermomyotome to incorporate exclusively into the developing dorsal aorta. Whereas SDECs lack hematopoietic potential they act as a local niche to support the emergence of HSPCs from neighboring hemogenic endothelium. Thus at least three subsets of ECs contribute to the developing dorsal aorta: vascular ECs hemogenic ECs and SDECs. Taken together our findings indicate that the distinct spatial origins of endothelial precursors dictate different cellular potentials within the developing dorsal aorta. | ENA FIRST PUBLIC:2023 08 01|ENA LAST UPDATE:2023 08 01 | Protocols: Dechorionated embryos were collected at defined stages dissociated by pipetting centrifugation at 300g for 5 minutes washed once with PBS and sorted by FACS. Total cell concentration and viability were determined using a TC20 Automated Cell Counter Bio Rad. Samples were then re suspended in 1x PBS with 0.05% BSA at 800 3000 cells per ml. Samples were loaded on the 10x Chromium system with a target of 4 000 cells. Chromium Single Cell 3ʹ GEM Library & Gel Bead Kit v2 or v3 was used for library preparation. Samples were loaded on the 10x Chromium system with a target of 4 000 cells. Chromium Single Cell 3ʹ GEM Library & Gel Bead Kit v2 or v3 was used for library preparation. | fli1tp1 24hpf | SAMEA114192246 | Institute of Molecular Genetics of the Czech Academy of Sciences | ENA FIRST PUBLIC:2023 08 01T00:17:16Z|ENA LAST UPDATE:2023 08 01T00:17:16Z|External Id:SAMEA114192246|INSDC center alias:Institute of Molecular Genetics of the Czech Academy of Sciences|INSDC center name:Institute of Molecular Genetics of the Czech Academy of Sciences|INSDC first public:2023 08 01T00:17:16Z|INSDC last update:2023 08 01T00:17:16Z|INSDC status:public|Submitter Id:E MTAB 13196:fli1tp1 24hpf|age:24|broker name:ArrayExpress|cell type:endothelial cell|collection date:not collected|common name:zebrafish|developmental stage:24 hpf|genotype:fli1:DsRed tp1:eGFP|geographic location country and/or sea:not collected|immunophenotype:fli1:DsRed+ tp1:eGFP+|isolate:not applicable|organism part:embryo|sample name:E MTAB 13196:fli1tp1 24hpf|scientific name:Danio rerio|strain:fli1:DsRed tp1:eGFP | Illumina HiSeq 2500 paired end sequencing; Single cell RNA seq 10x Chromium of FACS sorted drl:H2B Dendra2 etv2:Kaede fli1:DsRed and tp1:eGFP transgenic zebrafish embryos | E MTAB 13196:fli1tp1 24hpf p | fli1tp1 24hpf p | Single cell RNA seq 10x Chromium of FACS sorted drl:H2B Dendra2 etv2:Kaede fli1:DsRed and tp1:eGFP transgenic zebrafish embryos | Dechorionated embryos were collected at defined stages dissociated by pipetting centrifugation at 300g for 5 minutes washed once with PBS and sorted by FACS. Total cell concentration and viability were determined using a TC20 Automated Cell Counter Bio Rad. Samples were then re suspended in 1x PBS with 0.05% BSA at 800 3000 cells per ml. Samples were loaded on the 10x Chromium system with a target of 4 000 cells. Chromium Single Cell 3สน GEM Library & Gel Bead Kit v2 or v3 was used for library preparation. Samples were loaded on the 10x Chromium system with a target of 4 000 cells. Chromium Single Cell 3สน GEM Library & Gel Bead Kit v2 or v3 was used for library preparation. | RNA-Seq | TRANSCRIPTOMIC SINGLE CELL | Oligo-dT | PAIRED | ILLUMINA | Illumina HiSeq 2500 | ERP149744 | Illumina HiSeq 2500 paired end sequencing; Single cell RNA seq 10x Chromium of FACS sorted drl:H2B Dendra2 etv2:Kaede fli1:DsRed and tp1:eGFP transgenic zebrafish embryos | ENA FIRST PUBLIC:2023 08 01|ENA LAST UPDATE:2023 08 01 | FliTp124hpf_S24_L001_R1_001.fastq.gz FliTp124hpf_S24_L001_R2_001.fastq.gz | fastq fastq | 558365976.0 | 4431476.0 | E MTAB 13196:FliTp124hpf S24 L001 | 0:28 1:98 | A:156054630;C:128109738;G:127885708;T:146256146;N:59754 | 28 | 98 | 156054630 | 128109738 | 127885708 | 146256146 | 59754 | ERX11157721 | ERS16172939 | ERA25628220 | Institute of Molecular Genetics of the Czech Academy of Sciences|European Nucleotide Archive | Institute of Molecular Genetics of the Czech Academy of Sciences|European Nucleotide Archive | 2 | 0.00345 | 0.8879 | 0.00104 | 0.07718 | 0.99322 | 0.84364 | 0.38266 | 0.49193 | 28 | 98 | T | B | sc-like readlen | illumina | hiseq_era | unknown | poly_a | unknown | sc | single_cell_droplet | 10x | Czech Republic | 2023-08-01 | Pharyngula | Embryo | Embryo Imprecise | All anatomical structures | ||||||||||||||
| 11765 | 11765 | ERR11758635 | ERX11157720 | ERS16172938 | ERP149744 | PRJEB64563 | Single cell RNA seq 10x Chromium of FACS sorted drl:H2B Dendra2 etv2:Kaede fli1:DsRed and tp1:eGFP transgenic zebrafish embryos | E-MTAB-13196 | Transcriptome Analysis | Development of the dorsal aorta is a key step in the establishment of the adult blood forming system since hematopoietic stem and progenitor cells HSPCs arise from ventral aortic endothelium in all vertebrate animals studied. Work in zebrafish has demonstrated that arterial and venous endothelial precursors arise from distinct subsets of lateral plate mesoderm. Here we profile the transcriptome of the earliest detectable endothelial cells ECs during zebrafish embryogenesis to demonstrate that tissue specific EC programs initiate much earlier than previously appreciated by the end of gastrulation. Classic studies in the chick embryo showed that paraxial mesoderm generates a subset of somite derived endothelial cells SDECs that incorporate into the dorsal aorta to replace HSPCs as they exit the aorta and enter circulation. We describe a conserved program in the zebrafish where a rare population of endothelial precursors delaminates from the dermomyotome to incorporate exclusively into the developing dorsal aorta. Whereas SDECs lack hematopoietic potential they act as a local niche to support the emergence of HSPCs from neighboring hemogenic endothelium. Thus at least three subsets of ECs contribute to the developing dorsal aorta: vascular ECs hemogenic ECs and SDECs. Taken together our findings indicate that the distinct spatial origins of endothelial precursors dictate different cellular potentials within the developing dorsal aorta. | ENA FIRST PUBLIC:2023 08 01|ENA LAST UPDATE:2023 08 01 | Protocols: Dechorionated embryos were collected at defined stages dissociated by pipetting centrifugation at 300g for 5 minutes washed once with PBS and sorted by FACS. Total cell concentration and viability were determined using a TC20 Automated Cell Counter Bio Rad. Samples were then re suspended in 1x PBS with 0.05% BSA at 800 3000 cells per ml. Samples were loaded on the 10x Chromium system with a target of 4 000 cells. Chromium Single Cell 3ʹ GEM Library & Gel Bead Kit v2 or v3 was used for library preparation. Samples were loaded on the 10x Chromium system with a target of 4 000 cells. Chromium Single Cell 3ʹ GEM Library & Gel Bead Kit v2 or v3 was used for library preparation. | etv2 wt 22hpf | SAMEA114192245 | Institute of Molecular Genetics of the Czech Academy of Sciences | ENA FIRST PUBLIC:2023 08 01T00:17:16Z|ENA LAST UPDATE:2023 08 01T00:17:16Z|External Id:SAMEA114192245|INSDC center alias:Institute of Molecular Genetics of the Czech Academy of Sciences|INSDC center name:Institute of Molecular Genetics of the Czech Academy of Sciences|INSDC first public:2023 08 01T00:17:16Z|INSDC last update:2023 08 01T00:17:16Z|INSDC status:public|Submitter Id:E MTAB 13196:etv2 wt 22hpf|age:24|broker name:ArrayExpress|cell type:endothelial cell|collection date:not collected|common name:zebrafish|developmental stage:24 hpf|genotype:etv2:Kaede|geographic location country and/or sea:not collected|immunophenotype:etv2:Kaede+|isolate:not applicable|organism part:embryo|sample name:E MTAB 13196:etv2 wt 22hpf|scientific name:Danio rerio|strain:etv2:Kaede | Illumina HiSeq 2500 paired end sequencing; Single cell RNA seq 10x Chromium of FACS sorted drl:H2B Dendra2 etv2:Kaede fli1:DsRed and tp1:eGFP transgenic zebrafish embryos | E MTAB 13196:etv2 wt 22hpf p | etv2 wt 22hpf p | Single cell RNA seq 10x Chromium of FACS sorted drl:H2B Dendra2 etv2:Kaede fli1:DsRed and tp1:eGFP transgenic zebrafish embryos | Dechorionated embryos were collected at defined stages dissociated by pipetting centrifugation at 300g for 5 minutes washed once with PBS and sorted by FACS. Total cell concentration and viability were determined using a TC20 Automated Cell Counter Bio Rad. Samples were then re suspended in 1x PBS with 0.05% BSA at 800 3000 cells per ml. Samples were loaded on the 10x Chromium system with a target of 4 000 cells. Chromium Single Cell 3สน GEM Library & Gel Bead Kit v2 or v3 was used for library preparation. Samples were loaded on the 10x Chromium system with a target of 4 000 cells. Chromium Single Cell 3สน GEM Library & Gel Bead Kit v2 or v3 was used for library preparation. | RNA-Seq | TRANSCRIPTOMIC SINGLE CELL | Oligo-dT | PAIRED | ILLUMINA | Illumina HiSeq 2500 | ERP149744 | Illumina HiSeq 2500 paired end sequencing; Single cell RNA seq 10x Chromium of FACS sorted drl:H2B Dendra2 etv2:Kaede fli1:DsRed and tp1:eGFP transgenic zebrafish embryos | ENA FIRST PUBLIC:2023 08 01|ENA LAST UPDATE:2023 08 01 | Etv2Kaede_WT_S6_L001_R1_001.fastq.gz Etv2Kaede_WT_S6_L001_R2_001.fastq.gz | fastq fastq | 1144014102.0 | 9079477.0 | E MTAB 13196:Etv2Kaede WT S6 L001 | 0:28 1:98 | A:316366230;C:260322813;G:259447166;T:307680623;N:197270 | 28 | 98 | 316366230 | 260322813 | 259447166 | 307680623 | 197270 | ERX11157720 | ERS16172938 | ERA25628220 | Institute of Molecular Genetics of the Czech Academy of Sciences|European Nucleotide Archive | Institute of Molecular Genetics of the Czech Academy of Sciences|European Nucleotide Archive | 2 | 0.00336 | 0.89267 | 0.00137 | 0.13734 | 0.99545 | 0.81957 | 0.3734 | 0.53458 | 28 | 98 | T | B | sc-like readlen | illumina | hiseq_era | unknown | poly_a | unknown | sc | single_cell_droplet | 10x | Czech Republic | 2023-08-01 | Pharyngula | Embryo | Embryo Imprecise | All anatomical structures | ||||||||||||||
| 11766 | 11766 | ERR11758622 | ERX11157720 | ERS16172938 | ERP149744 | PRJEB64563 | Single cell RNA seq 10x Chromium of FACS sorted drl:H2B Dendra2 etv2:Kaede fli1:DsRed and tp1:eGFP transgenic zebrafish embryos | E-MTAB-13196 | Transcriptome Analysis | Development of the dorsal aorta is a key step in the establishment of the adult blood forming system since hematopoietic stem and progenitor cells HSPCs arise from ventral aortic endothelium in all vertebrate animals studied. Work in zebrafish has demonstrated that arterial and venous endothelial precursors arise from distinct subsets of lateral plate mesoderm. Here we profile the transcriptome of the earliest detectable endothelial cells ECs during zebrafish embryogenesis to demonstrate that tissue specific EC programs initiate much earlier than previously appreciated by the end of gastrulation. Classic studies in the chick embryo showed that paraxial mesoderm generates a subset of somite derived endothelial cells SDECs that incorporate into the dorsal aorta to replace HSPCs as they exit the aorta and enter circulation. We describe a conserved program in the zebrafish where a rare population of endothelial precursors delaminates from the dermomyotome to incorporate exclusively into the developing dorsal aorta. Whereas SDECs lack hematopoietic potential they act as a local niche to support the emergence of HSPCs from neighboring hemogenic endothelium. Thus at least three subsets of ECs contribute to the developing dorsal aorta: vascular ECs hemogenic ECs and SDECs. Taken together our findings indicate that the distinct spatial origins of endothelial precursors dictate different cellular potentials within the developing dorsal aorta. | ENA FIRST PUBLIC:2023 08 01|ENA LAST UPDATE:2023 08 01 | Protocols: Dechorionated embryos were collected at defined stages dissociated by pipetting centrifugation at 300g for 5 minutes washed once with PBS and sorted by FACS. Total cell concentration and viability were determined using a TC20 Automated Cell Counter Bio Rad. Samples were then re suspended in 1x PBS with 0.05% BSA at 800 3000 cells per ml. Samples were loaded on the 10x Chromium system with a target of 4 000 cells. Chromium Single Cell 3ʹ GEM Library & Gel Bead Kit v2 or v3 was used for library preparation. Samples were loaded on the 10x Chromium system with a target of 4 000 cells. Chromium Single Cell 3ʹ GEM Library & Gel Bead Kit v2 or v3 was used for library preparation. | etv2 wt 22hpf | SAMEA114192245 | Institute of Molecular Genetics of the Czech Academy of Sciences | ENA FIRST PUBLIC:2023 08 01T00:17:16Z|ENA LAST UPDATE:2023 08 01T00:17:16Z|External Id:SAMEA114192245|INSDC center alias:Institute of Molecular Genetics of the Czech Academy of Sciences|INSDC center name:Institute of Molecular Genetics of the Czech Academy of Sciences|INSDC first public:2023 08 01T00:17:16Z|INSDC last update:2023 08 01T00:17:16Z|INSDC status:public|Submitter Id:E MTAB 13196:etv2 wt 22hpf|age:24|broker name:ArrayExpress|cell type:endothelial cell|collection date:not collected|common name:zebrafish|developmental stage:24 hpf|genotype:etv2:Kaede|geographic location country and/or sea:not collected|immunophenotype:etv2:Kaede+|isolate:not applicable|organism part:embryo|sample name:E MTAB 13196:etv2 wt 22hpf|scientific name:Danio rerio|strain:etv2:Kaede | Illumina HiSeq 2500 paired end sequencing; Single cell RNA seq 10x Chromium of FACS sorted drl:H2B Dendra2 etv2:Kaede fli1:DsRed and tp1:eGFP transgenic zebrafish embryos | E MTAB 13196:etv2 wt 22hpf p | etv2 wt 22hpf p | Single cell RNA seq 10x Chromium of FACS sorted drl:H2B Dendra2 etv2:Kaede fli1:DsRed and tp1:eGFP transgenic zebrafish embryos | Dechorionated embryos were collected at defined stages dissociated by pipetting centrifugation at 300g for 5 minutes washed once with PBS and sorted by FACS. Total cell concentration and viability were determined using a TC20 Automated Cell Counter Bio Rad. Samples were then re suspended in 1x PBS with 0.05% BSA at 800 3000 cells per ml. Samples were loaded on the 10x Chromium system with a target of 4 000 cells. Chromium Single Cell 3สน GEM Library & Gel Bead Kit v2 or v3 was used for library preparation. Samples were loaded on the 10x Chromium system with a target of 4 000 cells. Chromium Single Cell 3สน GEM Library & Gel Bead Kit v2 or v3 was used for library preparation. | RNA-Seq | TRANSCRIPTOMIC SINGLE CELL | Oligo-dT | PAIRED | ILLUMINA | Illumina HiSeq 2500 | ERP149744 | Illumina HiSeq 2500 paired end sequencing; Single cell RNA seq 10x Chromium of FACS sorted drl:H2B Dendra2 etv2:Kaede fli1:DsRed and tp1:eGFP transgenic zebrafish embryos | ENA FIRST PUBLIC:2023 08 01|ENA LAST UPDATE:2023 08 01 | Etv2Kaede_WT_S8_L001_R2_001.fastq.gz Etv2Kaede_WT_S8_L001_R1_001.fastq.gz | fastq fastq | 919270926.0 | 7295801.0 | E MTAB 13196:Etv2Kaede WT S8 L001 | 0:28 1:98 | A:259787542;C:207541571;G:206711907;T:245072713;N:157193 | 28 | 98 | 259787542 | 207541571 | 206711907 | 245072713 | 157193 | ERX11157720 | ERS16172938 | ERA25628220 | Institute of Molecular Genetics of the Czech Academy of Sciences|European Nucleotide Archive | Institute of Molecular Genetics of the Czech Academy of Sciences|European Nucleotide Archive | 2 | 0.00358 | 0.88757 | 0.00137 | 0.15368 | 0.99551 | 0.83378 | 0.36405 | 0.53733 | 28 | 98 | T | B | sc-like readlen | illumina | hiseq_era | unknown | poly_a | unknown | sc | single_cell_droplet | 10x | Czech Republic | 2023-08-01 | Pharyngula | Embryo | Embryo Imprecise | All anatomical structures | ||||||||||||||
| 11767 | 11767 | ERR11758644 | ERX11157720 | ERS16172938 | ERP149744 | PRJEB64563 | Single cell RNA seq 10x Chromium of FACS sorted drl:H2B Dendra2 etv2:Kaede fli1:DsRed and tp1:eGFP transgenic zebrafish embryos | E-MTAB-13196 | Transcriptome Analysis | Development of the dorsal aorta is a key step in the establishment of the adult blood forming system since hematopoietic stem and progenitor cells HSPCs arise from ventral aortic endothelium in all vertebrate animals studied. Work in zebrafish has demonstrated that arterial and venous endothelial precursors arise from distinct subsets of lateral plate mesoderm. Here we profile the transcriptome of the earliest detectable endothelial cells ECs during zebrafish embryogenesis to demonstrate that tissue specific EC programs initiate much earlier than previously appreciated by the end of gastrulation. Classic studies in the chick embryo showed that paraxial mesoderm generates a subset of somite derived endothelial cells SDECs that incorporate into the dorsal aorta to replace HSPCs as they exit the aorta and enter circulation. We describe a conserved program in the zebrafish where a rare population of endothelial precursors delaminates from the dermomyotome to incorporate exclusively into the developing dorsal aorta. Whereas SDECs lack hematopoietic potential they act as a local niche to support the emergence of HSPCs from neighboring hemogenic endothelium. Thus at least three subsets of ECs contribute to the developing dorsal aorta: vascular ECs hemogenic ECs and SDECs. Taken together our findings indicate that the distinct spatial origins of endothelial precursors dictate different cellular potentials within the developing dorsal aorta. | ENA FIRST PUBLIC:2023 08 01|ENA LAST UPDATE:2023 08 01 | Protocols: Dechorionated embryos were collected at defined stages dissociated by pipetting centrifugation at 300g for 5 minutes washed once with PBS and sorted by FACS. Total cell concentration and viability were determined using a TC20 Automated Cell Counter Bio Rad. Samples were then re suspended in 1x PBS with 0.05% BSA at 800 3000 cells per ml. Samples were loaded on the 10x Chromium system with a target of 4 000 cells. Chromium Single Cell 3ʹ GEM Library & Gel Bead Kit v2 or v3 was used for library preparation. Samples were loaded on the 10x Chromium system with a target of 4 000 cells. Chromium Single Cell 3ʹ GEM Library & Gel Bead Kit v2 or v3 was used for library preparation. | etv2 wt 22hpf | SAMEA114192245 | Institute of Molecular Genetics of the Czech Academy of Sciences | ENA FIRST PUBLIC:2023 08 01T00:17:16Z|ENA LAST UPDATE:2023 08 01T00:17:16Z|External Id:SAMEA114192245|INSDC center alias:Institute of Molecular Genetics of the Czech Academy of Sciences|INSDC center name:Institute of Molecular Genetics of the Czech Academy of Sciences|INSDC first public:2023 08 01T00:17:16Z|INSDC last update:2023 08 01T00:17:16Z|INSDC status:public|Submitter Id:E MTAB 13196:etv2 wt 22hpf|age:24|broker name:ArrayExpress|cell type:endothelial cell|collection date:not collected|common name:zebrafish|developmental stage:24 hpf|genotype:etv2:Kaede|geographic location country and/or sea:not collected|immunophenotype:etv2:Kaede+|isolate:not applicable|organism part:embryo|sample name:E MTAB 13196:etv2 wt 22hpf|scientific name:Danio rerio|strain:etv2:Kaede | Illumina HiSeq 2500 paired end sequencing; Single cell RNA seq 10x Chromium of FACS sorted drl:H2B Dendra2 etv2:Kaede fli1:DsRed and tp1:eGFP transgenic zebrafish embryos | E MTAB 13196:etv2 wt 22hpf p | etv2 wt 22hpf p | Single cell RNA seq 10x Chromium of FACS sorted drl:H2B Dendra2 etv2:Kaede fli1:DsRed and tp1:eGFP transgenic zebrafish embryos | Dechorionated embryos were collected at defined stages dissociated by pipetting centrifugation at 300g for 5 minutes washed once with PBS and sorted by FACS. Total cell concentration and viability were determined using a TC20 Automated Cell Counter Bio Rad. Samples were then re suspended in 1x PBS with 0.05% BSA at 800 3000 cells per ml. Samples were loaded on the 10x Chromium system with a target of 4 000 cells. Chromium Single Cell 3สน GEM Library & Gel Bead Kit v2 or v3 was used for library preparation. Samples were loaded on the 10x Chromium system with a target of 4 000 cells. Chromium Single Cell 3สน GEM Library & Gel Bead Kit v2 or v3 was used for library preparation. | RNA-Seq | TRANSCRIPTOMIC SINGLE CELL | Oligo-dT | PAIRED | ILLUMINA | Illumina HiSeq 2500 | ERP149744 | Illumina HiSeq 2500 paired end sequencing; Single cell RNA seq 10x Chromium of FACS sorted drl:H2B Dendra2 etv2:Kaede fli1:DsRed and tp1:eGFP transgenic zebrafish embryos | ENA FIRST PUBLIC:2023 08 01|ENA LAST UPDATE:2023 08 01 | Etv2Kaede_WT_S5_L001_R2_001.fastq.gz Etv2Kaede_WT_S5_L001_R1_001.fastq.gz | fastq fastq | 877658166.0 | 6965541.0 | E MTAB 13196:Etv2Kaede WT S5 L001 | 0:28 1:98 | A:243057461;C:199464116;G:198654377;T:236322700;N:159512 | 28 | 98 | 243057461 | 199464116 | 198654377 | 236322700 | 159512 | ERX11157720 | ERS16172938 | ERA25628220 | Institute of Molecular Genetics of the Czech Academy of Sciences|European Nucleotide Archive | Institute of Molecular Genetics of the Czech Academy of Sciences|European Nucleotide Archive | 2 | 0.00328 | 0.88916 | 0.00127 | 0.13782 | 0.99559 | 0.81925 | 0.38227 | 0.53623 | 28 | 98 | T | B | sc-like readlen | illumina | hiseq_era | unknown | poly_a | unknown | sc | single_cell_droplet | 10x | Czech Republic | 2023-08-01 | Pharyngula | Embryo | Embryo Imprecise | All anatomical structures | ||||||||||||||
| 11768 | 11768 | ERR11758628 | ERX11157720 | ERS16172938 | ERP149744 | PRJEB64563 | Single cell RNA seq 10x Chromium of FACS sorted drl:H2B Dendra2 etv2:Kaede fli1:DsRed and tp1:eGFP transgenic zebrafish embryos | E-MTAB-13196 | Transcriptome Analysis | Development of the dorsal aorta is a key step in the establishment of the adult blood forming system since hematopoietic stem and progenitor cells HSPCs arise from ventral aortic endothelium in all vertebrate animals studied. Work in zebrafish has demonstrated that arterial and venous endothelial precursors arise from distinct subsets of lateral plate mesoderm. Here we profile the transcriptome of the earliest detectable endothelial cells ECs during zebrafish embryogenesis to demonstrate that tissue specific EC programs initiate much earlier than previously appreciated by the end of gastrulation. Classic studies in the chick embryo showed that paraxial mesoderm generates a subset of somite derived endothelial cells SDECs that incorporate into the dorsal aorta to replace HSPCs as they exit the aorta and enter circulation. We describe a conserved program in the zebrafish where a rare population of endothelial precursors delaminates from the dermomyotome to incorporate exclusively into the developing dorsal aorta. Whereas SDECs lack hematopoietic potential they act as a local niche to support the emergence of HSPCs from neighboring hemogenic endothelium. Thus at least three subsets of ECs contribute to the developing dorsal aorta: vascular ECs hemogenic ECs and SDECs. Taken together our findings indicate that the distinct spatial origins of endothelial precursors dictate different cellular potentials within the developing dorsal aorta. | ENA FIRST PUBLIC:2023 08 01|ENA LAST UPDATE:2023 08 01 | Protocols: Dechorionated embryos were collected at defined stages dissociated by pipetting centrifugation at 300g for 5 minutes washed once with PBS and sorted by FACS. Total cell concentration and viability were determined using a TC20 Automated Cell Counter Bio Rad. Samples were then re suspended in 1x PBS with 0.05% BSA at 800 3000 cells per ml. Samples were loaded on the 10x Chromium system with a target of 4 000 cells. Chromium Single Cell 3ʹ GEM Library & Gel Bead Kit v2 or v3 was used for library preparation. Samples were loaded on the 10x Chromium system with a target of 4 000 cells. Chromium Single Cell 3ʹ GEM Library & Gel Bead Kit v2 or v3 was used for library preparation. | etv2 wt 22hpf | SAMEA114192245 | Institute of Molecular Genetics of the Czech Academy of Sciences | ENA FIRST PUBLIC:2023 08 01T00:17:16Z|ENA LAST UPDATE:2023 08 01T00:17:16Z|External Id:SAMEA114192245|INSDC center alias:Institute of Molecular Genetics of the Czech Academy of Sciences|INSDC center name:Institute of Molecular Genetics of the Czech Academy of Sciences|INSDC first public:2023 08 01T00:17:16Z|INSDC last update:2023 08 01T00:17:16Z|INSDC status:public|Submitter Id:E MTAB 13196:etv2 wt 22hpf|age:24|broker name:ArrayExpress|cell type:endothelial cell|collection date:not collected|common name:zebrafish|developmental stage:24 hpf|genotype:etv2:Kaede|geographic location country and/or sea:not collected|immunophenotype:etv2:Kaede+|isolate:not applicable|organism part:embryo|sample name:E MTAB 13196:etv2 wt 22hpf|scientific name:Danio rerio|strain:etv2:Kaede | Illumina HiSeq 2500 paired end sequencing; Single cell RNA seq 10x Chromium of FACS sorted drl:H2B Dendra2 etv2:Kaede fli1:DsRed and tp1:eGFP transgenic zebrafish embryos | E MTAB 13196:etv2 wt 22hpf p | etv2 wt 22hpf p | Single cell RNA seq 10x Chromium of FACS sorted drl:H2B Dendra2 etv2:Kaede fli1:DsRed and tp1:eGFP transgenic zebrafish embryos | Dechorionated embryos were collected at defined stages dissociated by pipetting centrifugation at 300g for 5 minutes washed once with PBS and sorted by FACS. Total cell concentration and viability were determined using a TC20 Automated Cell Counter Bio Rad. Samples were then re suspended in 1x PBS with 0.05% BSA at 800 3000 cells per ml. Samples were loaded on the 10x Chromium system with a target of 4 000 cells. Chromium Single Cell 3สน GEM Library & Gel Bead Kit v2 or v3 was used for library preparation. Samples were loaded on the 10x Chromium system with a target of 4 000 cells. Chromium Single Cell 3สน GEM Library & Gel Bead Kit v2 or v3 was used for library preparation. | RNA-Seq | TRANSCRIPTOMIC SINGLE CELL | Oligo-dT | PAIRED | ILLUMINA | Illumina HiSeq 2500 | ERP149744 | Illumina HiSeq 2500 paired end sequencing; Single cell RNA seq 10x Chromium of FACS sorted drl:H2B Dendra2 etv2:Kaede fli1:DsRed and tp1:eGFP transgenic zebrafish embryos | ENA FIRST PUBLIC:2023 08 01|ENA LAST UPDATE:2023 08 01 | Etv2Kaede_WT_S7_L001_R1_001.fastq.gz Etv2Kaede_WT_S7_L001_R2_001.fastq.gz | fastq fastq | 930443598.0 | 7384473.0 | E MTAB 13196:Etv2Kaede WT S7 L001 | 0:28 1:98 | A:258539610;C:211017108;G:209074684;T:251641766;N:170430 | 28 | 98 | 258539610 | 211017108 | 209074684 | 251641766 | 170430 | ERX11157720 | ERS16172938 | ERA25628220 | Institute of Molecular Genetics of the Czech Academy of Sciences|European Nucleotide Archive | Institute of Molecular Genetics of the Czech Academy of Sciences|European Nucleotide Archive | 2 | 0.00355 | 0.88938 | 0.00148 | 0.14297 | 0.99569 | 0.83358 | 0.35626 | 0.53314 | 28 | 98 | T | B | sc-like readlen | illumina | hiseq_era | unknown | poly_a | unknown | sc | single_cell_droplet | 10x | Czech Republic | 2023-08-01 | Pharyngula | Embryo | Embryo Imprecise | All anatomical structures | ||||||||||||||
| 29721 | 29721 | SRR27485666 | SRX23156883 | SRS20107304 | SRP482074 | PRJNA1061456 | tRAM seq: tRNA abundance and modification analysis during zebrafish embryo development | PRJNA1061456 | Other | 24h R2 | strain:TLAB fish|isolate:NA|breed:cross of zebrafish AB and the natural variant TL Tupfel Longfin|cultivar:NA|ecotype:NA|dev stage:24 hpf|collection date:2022|geo loc name:Austria|sex:mixed|tissue:whole embryo|replicate:2|BioSampleModel:Model organism or animal | mRNA seq of zebrafish: embryo 24 hpf rep4 | EV06007 | EV06007 | RNA was extracted with Trizol and processed with QuantSeq three prime mRNA Seq Library Prep Kit FWD for Illumina Lexogen. 500 ng total RNA per sample. Single indexed QuantSeq libraries were QC checked on a Bioanalyzer 2100 Agilent using a High Sensitivity DNA Kit for correct insert size and quantified using Qubit dsDNA HS Assay Invitrogen. Pooled libraries were sequenced on a NextSeq500 instrument Illumina in 1x75bp single end sequencing mode | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | NextSeq 500 | SRP482074 | EV06007.R1.fastq.gz | fastq | 758984715.0 | 10062029.0 | EV06007.R1.fastq.gz | 0:75.43 | A:221215464;C:148997667;G:167225079;T:221520231;N:26274 | 75 | 221215464 | 148997667 | 167225079 | 221520231 | 26274 | SRX23156883 | SRS20107304 | SRA1783314 | Medical University of Vienna|Cell and Developmental Biology | Medical University of Vienna | 1 | 0.90914 | 0.18457 | 0.77782 | 0.38818 | 75 | B | usable mapping rate | illumina | nextseq | 3prime | poly_a | lexogen | bulk | unknown | unknown | Austria | 2024-01-11 | Pharyngula | Embryo | Whole Organism | All anatomical structures | |||||||||||||||||||||||||||||
| 29726 | 29726 | SRR27485671 | SRX23156878 | SRS20107299 | SRP482074 | PRJNA1061456 | tRAM seq: tRNA abundance and modification analysis during zebrafish embryo development | PRJNA1061456 | Other | 24h R3 | strain:TLAB fish|isolate:NA|breed:cross of zebrafish AB and the natural variant TL Tupfel Longfin|cultivar:NA|ecotype:NA|dev stage:24 hpf|collection date:2022|geo loc name:Austria|sex:mixed|tissue:whole embryo|replicate:3|BioSampleModel:Model organism or animal | mRNA seq of zebrafish: embryo 24 hpf rep4 | EV06014 | EV06014 | RNA was extracted with Trizol and processed with QuantSeq three prime mRNA Seq Library Prep Kit FWD for Illumina Lexogen. 500 ng total RNA per sample. Single indexed QuantSeq libraries were QC checked on a Bioanalyzer 2100 Agilent using a High Sensitivity DNA Kit for correct insert size and quantified using Qubit dsDNA HS Assay Invitrogen. Pooled libraries were sequenced on a NextSeq500 instrument Illumina in 1x75bp single end sequencing mode | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | NextSeq 500 | SRP482074 | EV06014.R1.fastq.gz | fastq | 708984998.0 | 9425445.0 | EV06014.R1.fastq.gz | 0:75.22 | A:219626080;C:141270902;G:161722995;T:186246245;N:118776 | 75 | 219626080 | 141270902 | 161722995 | 186246245 | 118776 | SRX23156878 | SRS20107299 | SRA1783314 | Medical University of Vienna|Cell and Developmental Biology | Medical University of Vienna | 1 | 0.84881 | 0.17975 | 0.79693 | 0.44149 | 76 | B | usable mapping rate | illumina | nextseq | 3prime | poly_a | lexogen | bulk | unknown | unknown | Austria | 2024-01-11 | Pharyngula | Embryo | Whole Organism | All anatomical structures | |||||||||||||||||||||||||||||
| 29732 | 29732 | SRR27477292 | SRX23148655 | SRS20099371 | SRP482074 | PRJNA1061456 | tRAM seq: tRNA abundance and modification analysis during zebrafish embryo development | PRJNA1061456 | Other | 24h R4 | strain:TLAB fish|isolate:NA|breed:cross of zebrafish AB and the natural variant TL Tupfel Longfin|cultivar:NA|ecotype:NA|dev stage:24 hpf|collection date:2022|geo loc name:Austria|sex:mixed|tissue:whole embryo|replicate:4|BioSampleModel:Model organism or animal | mRNA seq of zebrafish: embryo 24 hpf rep4 | EV09007 | EV09007 | RNA was extracted with Trizol and processed with QuantSeq three prime mRNA Seq Library Prep Kit FWD for Illumina Lexogen. 500 ng total RNA per sample. Single indexed QuantSeq libraries were QC checked on a Bioanalyzer 2100 Agilent using a High Sensitivity DNA Kit for correct insert size and quantified using Qubit dsDNA HS Assay Invitrogen. Pooled libraries were sequenced on a NextSeq500 instrument Illumina in 1x75bp single end sequencing mode | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | NextSeq 500 | SRP482074 | EV09007.R1.fastq.gz | fastq | 693872355.0 | 9217545.0 | EV09007.R1.fastq.gz | 0:75.28 | A:207207066;C:136288214;G:150585299;T:199742228;N:49548 | 75 | 207207066 | 136288214 | 150585299 | 199742228 | 49548 | SRX23148655 | SRS20099371 | SRA1782413 | Medical University of Vienna|Cell and Developmental Biology | Medical University of Vienna | 1 | 0.89376 | 0.11471 | 0.79192 | 0.60125 | 76 | B | usable mapping rate | illumina | nextseq | 3prime | poly_a | lexogen | bulk | unknown | unknown | Austria | 2024-01-10 | Pharyngula | Embryo | Whole Organism | All anatomical structures | |||||||||||||||||||||||||||||
| 34345 | 34345 | SRR31677713 | SRX27040110 | SRS23503219 | SRP550762 | PRJNA1196411 | Danio rerio Raw sequence reads | PRJNA1196411 | Other | RNA seq of gata6 mutants and siblings | mut3 30hpf | isolate:not collected|dev stage:30hpf|collection date:2024 02 20|geo loc name:China: chongqing|sex:not applicable|tissue:mut3 30hpf whole embryo|BioSampleModel:Model organism or animal | 30hpf mut3 | sample6 | sample6 | 30hpf whole embryo | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | PAIRED | ILLUMINA | Illumina NovaSeq X Plus | SRP550762 | 30hpf mut3_1.fq.gz 30hpf mut3_2.fq.gz | fastq fastq | 6943287600.0 | 23144292.0 | 30hpf mut3 1.fq.gz | 0:150 1:150 | A:1880946993;C:1612371130;G:1588213310;T:1861395696;N:360471 | 150 | 150 | 1880946993 | 1612371130 | 1588213310 | 1861395696 | 360471 | SRX27040110 | SRS23503219 | SRA2031942 | Chongqing Institute of Green and Intelligent Technology , Chinese Academy of Sciences|Research Center of Stem Cells and Ageing | Chongqing Institute of Green and Intelligent Technology , Chinese Academy of Sciences | B | B | biological fallback assumption | illumina | novaseq_era | unknown | poly_a | unknown | bulk | unknown | unknown | China | 2024-12-11 | Pharyngula | Embryo | Whole Organism | All anatomical structures | ||||||||||||||||||||||||||||||||
| 34346 | 34346 | SRR31677714 | SRX27040109 | SRS23503218 | SRP550762 | PRJNA1196411 | Danio rerio Raw sequence reads | PRJNA1196411 | Other | RNA seq of gata6 mutants and siblings | mut2 30hpf | isolate:not collected|dev stage:30hpf|collection date:2024 02 20|geo loc name:China: chongqing|sex:not applicable|tissue:mut2 30hpf whole embryo|BioSampleModel:Model organism or animal | 30hpf mut2 | sample5 | sample5 | 30hpf whole embryo | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | PAIRED | ILLUMINA | Illumina NovaSeq X Plus | SRP550762 | 30hpf mut2_1.fq.gz 30hpf mut2_2.fq.gz | fastq fastq | 7235344200.0 | 24117814.0 | 30hpf mut2 1.fq.gz | 0:150 1:150 | A:2039516350;C:1610256460;G:1577775149;T:2007409757;N:386484 | 150 | 150 | 2039516350 | 1610256460 | 1577775149 | 2007409757 | 386484 | SRX27040109 | SRS23503218 | SRA2031942 | Chongqing Institute of Green and Intelligent Technology , Chinese Academy of Sciences|Research Center of Stem Cells and Ageing | Chongqing Institute of Green and Intelligent Technology , Chinese Academy of Sciences | B | B | biological fallback assumption | illumina | novaseq_era | unknown | poly_a | unknown | bulk | unknown | unknown | China | 2024-12-11 | Pharyngula | Embryo | Whole Organism | All anatomical structures | ||||||||||||||||||||||||||||||||
| 34347 | 34347 | SRR31677715 | SRX27040108 | SRS23503217 | SRP550762 | PRJNA1196411 | Danio rerio Raw sequence reads | PRJNA1196411 | Other | RNA seq of gata6 mutants and siblings | mut1 30hpf | isolate:not collected|dev stage:30hpf|collection date:2024 02 20|geo loc name:China: chongqing|sex:not applicable|tissue:mut1 30hpf whole embryo|BioSampleModel:Model organism or animal | 30hpf mut1 | sample4 | sample4 | 30hpf whole embryo | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | PAIRED | ILLUMINA | Illumina NovaSeq X Plus | SRP550762 | 30hpf mut1_1.fq.gz 30hpf mut1_2.fq.gz | fastq fastq | 6625908300.0 | 22086361.0 | 30hpf mut1 1.fq.gz | 0:150 1:150 | A:1815071826;C:1519954980;G:1499263331;T:1791264958;N:353205 | 150 | 150 | 1815071826 | 1519954980 | 1499263331 | 1791264958 | 353205 | SRX27040108 | SRS23503217 | SRA2031942 | Chongqing Institute of Green and Intelligent Technology , Chinese Academy of Sciences|Research Center of Stem Cells and Ageing | Chongqing Institute of Green and Intelligent Technology , Chinese Academy of Sciences | B | B | biological fallback assumption | illumina | novaseq_era | unknown | poly_a | unknown | bulk | unknown | unknown | China | 2024-12-11 | Pharyngula | Embryo | Whole Organism | All anatomical structures | ||||||||||||||||||||||||||||||||
| 34348 | 34348 | SRR31677716 | SRX27040107 | SRS23503216 | SRP550762 | PRJNA1196411 | Danio rerio Raw sequence reads | PRJNA1196411 | Other | RNA seq of gata6 mutants and siblings | sib3 30hpf | isolate:not collected|dev stage:30hpf|collection date:2024 02 20|geo loc name:China: chongqing|sex:not applicable|tissue:sib3 30hpf whole embryo|BioSampleModel:Model organism or animal | 30hpf sib3 | sample3 | sample3 | 30hpf whole embryo | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | PAIRED | ILLUMINA | Illumina NovaSeq X Plus | SRP550762 | 30hpf sib3_1.fq.gz 30hpf sib3_2.fq.gz | fastq fastq | 6774228000.0 | 22580760.0 | 30hpf sib3 1.fq.gz | 0:150 1:150 | A:1887196046;C:1525955378;G:1501944328;T:1858775815;N:356433 | 150 | 150 | 1887196046 | 1525955378 | 1501944328 | 1858775815 | 356433 | SRX27040107 | SRS23503216 | SRA2031942 | Chongqing Institute of Green and Intelligent Technology , Chinese Academy of Sciences|Research Center of Stem Cells and Ageing | Chongqing Institute of Green and Intelligent Technology , Chinese Academy of Sciences | B | B | biological fallback assumption | illumina | novaseq_era | unknown | poly_a | unknown | bulk | unknown | unknown | China | 2024-12-11 | Pharyngula | Embryo | Whole Organism | All anatomical structures | ||||||||||||||||||||||||||||||||
| 34351 | 34351 | SRR31677719 | SRX27040104 | SRS23503213 | SRP550762 | PRJNA1196411 | Danio rerio Raw sequence reads | PRJNA1196411 | Other | RNA seq of gata6 mutants and siblings | sib2 30hpf | isolate:not collected|dev stage:30hpf|collection date:2024 02 20|geo loc name:China: chongqing|sex:not applicable|tissue:sib2 30hpf whole embryo|BioSampleModel:Model organism or animal | 30hpf sib2 | sample2 | sample2 | 30hpf whole embryo | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | PAIRED | ILLUMINA | Illumina NovaSeq X Plus | SRP550762 | 30hpf sib2_1.fq.gz 30hpf sib2_2.fq.gz | fastq fastq | 6508345200.0 | 21694484.0 | 30hpf sib2 1.fq.gz | 0:150 1:150 | A:1766203721;C:1507956441;G:1489359529;T:1744483985;N:341524 | 150 | 150 | 1766203721 | 1507956441 | 1489359529 | 1744483985 | 341524 | SRX27040104 | SRS23503213 | SRA2031942 | Chongqing Institute of Green and Intelligent Technology , Chinese Academy of Sciences|Research Center of Stem Cells and Ageing | Chongqing Institute of Green and Intelligent Technology , Chinese Academy of Sciences | B | B | biological fallback assumption | illumina | novaseq_era | unknown | poly_a | unknown | bulk | unknown | unknown | China | 2024-12-11 | Pharyngula | Embryo | Whole Organism | All anatomical structures | ||||||||||||||||||||||||||||||||
| 34352 | 34352 | SRR31677720 | SRX27040103 | SRS23503212 | SRP550762 | PRJNA1196411 | Danio rerio Raw sequence reads | PRJNA1196411 | Other | RNA seq of gata6 mutants and siblings | sib1 30hpf | isolate:not collected|dev stage:30hpf|collection date:2024 02 20|geo loc name:China: chongqing|sex:not applicable|tissue:sib1 30hpf whole embryo|BioSampleModel:Model organism or animal | 30hpf sib1 | sample1 | sample1 | 30hpf whole embryo | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | PAIRED | ILLUMINA | Illumina NovaSeq X Plus | SRP550762 | 30hpf sib1_1.fq.gz 30hpf sib1_2.fq.gz | fastq fastq | 6460412400.0 | 21534708.0 | 30hpf sib1 1.fq.gz | 0:150 1:150 | A:1743002257;C:1503750658;G:1488945374;T:1724378165;N:335946 | 150 | 150 | 1743002257 | 1503750658 | 1488945374 | 1724378165 | 335946 | SRX27040103 | SRS23503212 | SRA2031942 | Chongqing Institute of Green and Intelligent Technology , Chinese Academy of Sciences|Research Center of Stem Cells and Ageing | Chongqing Institute of Green and Intelligent Technology , Chinese Academy of Sciences | B | B | biological fallback assumption | illumina | novaseq_era | unknown | poly_a | unknown | bulk | unknown | unknown | China | 2024-12-11 | Pharyngula | Embryo | Whole Organism | All anatomical structures | ||||||||||||||||||||||||||||||||
| 35850 | 35850 | SRR33076649 | SRX28340997 | SRS24670562 | SRP577801 | PRJNA1249431 | DNACas | PRJNA1249431 | Other | Optimization of UV irradiation and PC&PS oligos for gene editing in zebrafish. | DNACas | strain:TAB5|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|age:not applicable|dev stage:24 hpf|collection date:2025 03 12|geo loc name:China|sex:pooled male and female|tissue:whole embryos|BioSampleModel:Model organism or animal | RNA seq of injected ABEUmax ex1 only for fig4 and figsupp10 | RNP&PCPSdark1 | RNP&PCPSdark1 | adapter | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | PAIRED | ILLUMINA | HiSeq X Ten | SRP577801 | RNP&PCPSdark1_R1.fastq.gz RNP&PCPSdark1_R2.fastq.gz | fastq fastq | 8848973400.0 | 29496578.0 | RNP&PCPSdark1 R1.fastq.gz | 0:150 1:150 | A:2354113378;C:2077073461;G:2087235148;T:2329940088;N:611325 | 150 | 150 | 2354113378 | 2077073461 | 2087235148 | 2329940088 | 611325 | SRX28340997 | SRS24670562 | SRA2110225 | South China Normal University|School of Life Sciences | South China Normal University | B | B | biological fallback assumption | illumina | hiseq_era | unknown | poly_a | unknown | bulk | unknown | unknown | China | 2025-04-11 | Pharyngula | Embryo | Whole Organism | All anatomical structures | ||||||||||||||||||||||||||||||||
| 35851 | 35851 | SRR33076650 | SRX28340996 | SRS24670562 | SRP577801 | PRJNA1249431 | DNACas | PRJNA1249431 | Other | Optimization of UV irradiation and PC&PS oligos for gene editing in zebrafish. | DNACas | strain:TAB5|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|age:not applicable|dev stage:24 hpf|collection date:2025 03 12|geo loc name:China|sex:pooled male and female|tissue:whole embryos|BioSampleModel:Model organism or animal | RNA seq of injected ABEUmax only for fig4 and figsupp10 | RNP3 | RNP3 | adapter | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | PAIRED | ILLUMINA | HiSeq X Ten | SRP577801 | RNP3_R1.fastq.gz RNP3_R2.fastq.gz | fastq fastq | 7794868800.0 | 25982896.0 | RNP3 R1.fastq.gz | 0:150 1:150 | A:2087730416;C:1817769012;G:1824246947;T:2064559805;N:562620 | 150 | 150 | 2087730416 | 1817769012 | 1824246947 | 2064559805 | 562620 | SRX28340996 | SRS24670562 | SRA2110225 | South China Normal University|School of Life Sciences | South China Normal University | B | B | biological fallback assumption | illumina | hiseq_era | unknown | poly_a | unknown | bulk | unknown | unknown | China | 2025-04-11 | Pharyngula | Embryo | Whole Organism | All anatomical structures | ||||||||||||||||||||||||||||||||
| 35852 | 35852 | SRR33076651 | SRX28340995 | SRS24670562 | SRP577801 | PRJNA1249431 | DNACas | PRJNA1249431 | Other | Optimization of UV irradiation and PC&PS oligos for gene editing in zebrafish. | DNACas | strain:TAB5|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|age:not applicable|dev stage:24 hpf|collection date:2025 03 12|geo loc name:China|sex:pooled male and female|tissue:whole embryos|BioSampleModel:Model organism or animal | RNA seq of injected ABEUmax only for fig4 and figsupp10 | RNP2 | RNP2 | adapter | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | PAIRED | ILLUMINA | HiSeq X Ten | SRP577801 | RNP2_R1.fastq.gz RNP2_R2.fastq.gz | fastq fastq | 9825604500.0 | 32752015.0 | RNP2 R1.fastq.gz | 0:150 1:150 | A:2635309510;C:2288423751;G:2294106654;T:2607073110;N:691475 | 150 | 150 | 2635309510 | 2288423751 | 2294106654 | 2607073110 | 691475 | SRX28340995 | SRS24670562 | SRA2110225 | South China Normal University|School of Life Sciences | South China Normal University | B | B | biological fallback assumption | illumina | hiseq_era | unknown | poly_a | unknown | bulk | unknown | unknown | China | 2025-04-11 | Pharyngula | Embryo | Whole Organism | All anatomical structures | ||||||||||||||||||||||||||||||||
| 35853 | 35853 | SRR33076652 | SRX28340994 | SRS24670562 | SRP577801 | PRJNA1249431 | DNACas | PRJNA1249431 | Other | Optimization of UV irradiation and PC&PS oligos for gene editing in zebrafish. | DNACas | strain:TAB5|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|age:not applicable|dev stage:24 hpf|collection date:2025 03 12|geo loc name:China|sex:pooled male and female|tissue:whole embryos|BioSampleModel:Model organism or animal | RNA seq of injected ABEUmax only for fig4 and figsupp10 | RNP1 | RNP1 | adapter | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | PAIRED | ILLUMINA | HiSeq X Ten | SRP577801 | RNP1_R1.fastq.gz RNP1_R2.fastq.gz | fastq fastq | 7515618000.0 | 25052060.0 | RNP1 R1.fastq.gz | 0:150 1:150 | A:2010030462;C:1755973960;G:1760594465;T:1988493578;N:525535 | 150 | 150 | 2010030462 | 1755973960 | 1760594465 | 1988493578 | 525535 | SRX28340994 | SRS24670562 | SRA2110225 | South China Normal University|School of Life Sciences | South China Normal University | B | B | biological fallback assumption | illumina | hiseq_era | unknown | poly_a | unknown | bulk | unknown | unknown | China | 2025-04-11 | Pharyngula | Embryo | Whole Organism | All anatomical structures | ||||||||||||||||||||||||||||||||
| 35854 | 35854 | SRR33076653 | SRX28340993 | SRS24670562 | SRP577801 | PRJNA1249431 | DNACas | PRJNA1249431 | Other | Optimization of UV irradiation and PC&PS oligos for gene editing in zebrafish. | DNACas | strain:TAB5|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|age:not applicable|dev stage:24 hpf|collection date:2025 03 12|geo loc name:China|sex:pooled male and female|tissue:whole embryos|BioSampleModel:Model organism or animal | RNA seq of injected ABE8e only for fig4 and figsupp10 | UV60s3 | UV60s3 | adapter | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | PAIRED | ILLUMINA | HiSeq X Ten | SRP577801 | UV60s3_R1.fastq.gz UV60s3_R2.fastq.gz | fastq fastq | 7232854500.0 | 24109515.0 | UV60s3 R1.fastq.gz | 0:150 1:150 | A:1926683661;C:1696217715;G:1707652822;T:1902280305;N:19997 | 150 | 150 | 1926683661 | 1696217715 | 1707652822 | 1902280305 | 19997 | SRX28340993 | SRS24670562 | SRA2110225 | South China Normal University|School of Life Sciences | South China Normal University | B | B | biological fallback assumption | illumina | hiseq_era | unknown | poly_a | unknown | bulk | unknown | unknown | China | 2025-04-11 | Pharyngula | Embryo | Whole Organism | All anatomical structures | ||||||||||||||||||||||||||||||||
| 35855 | 35855 | SRR33076654 | SRX28340992 | SRS24670562 | SRP577801 | PRJNA1249431 | DNACas | PRJNA1249431 | Other | Optimization of UV irradiation and PC&PS oligos for gene editing in zebrafish. | DNACas | strain:TAB5|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|age:not applicable|dev stage:24 hpf|collection date:2025 03 12|geo loc name:China|sex:pooled male and female|tissue:whole embryos|BioSampleModel:Model organism or animal | RNA seq of injected ABE8e only for fig4 and figsupp10 | UV60s2 | UV60s2 | adapter | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | PAIRED | ILLUMINA | HiSeq X Ten | SRP577801 | UV60s2_R1.fastq.gz UV60s2_R2.fastq.gz | fastq fastq | 7204532400.0 | 24015108.0 | UV60s2 R1.fastq.gz | 0:150 1:150 | A:1892484387;C:1715527707;G:1726571310;T:1869928245;N:20751 | 150 | 150 | 1892484387 | 1715527707 | 1726571310 | 1869928245 | 20751 | SRX28340992 | SRS24670562 | SRA2110225 | South China Normal University|School of Life Sciences | South China Normal University | B | B | biological fallback assumption | illumina | hiseq_era | unknown | poly_a | unknown | bulk | unknown | unknown | China | 2025-04-11 | Pharyngula | Embryo | Whole Organism | All anatomical structures | ||||||||||||||||||||||||||||||||
| 35856 | 35856 | SRR33076655 | SRX28340991 | SRS24670562 | SRP577801 | PRJNA1249431 | DNACas | PRJNA1249431 | Other | Optimization of UV irradiation and PC&PS oligos for gene editing in zebrafish. | DNACas | strain:TAB5|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|age:not applicable|dev stage:24 hpf|collection date:2025 03 12|geo loc name:China|sex:pooled male and female|tissue:whole embryos|BioSampleModel:Model organism or animal | RNA seq of injected ABE8e only for fig4 and figsupp10 | UV60s1 | UV60s1 | adapter | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | PAIRED | ILLUMINA | HiSeq X Ten | SRP577801 | UV60s1_R1.fastq.gz UV60s1_R2.fastq.gz | fastq fastq | 6373299600.0 | 21244332.0 | UV60s1 R1.fastq.gz | 0:150 1:150 | A:1706355288;C:1485803278;G:1494740583;T:1686382476;N:17975 | 150 | 150 | 1706355288 | 1485803278 | 1494740583 | 1686382476 | 17975 | SRX28340991 | SRS24670562 | SRA2110225 | South China Normal University|School of Life Sciences | South China Normal University | B | B | biological fallback assumption | illumina | hiseq_era | unknown | poly_a | unknown | bulk | unknown | unknown | China | 2025-04-11 | Pharyngula | Embryo | Whole Organism | All anatomical structures | ||||||||||||||||||||||||||||||||
| 35857 | 35857 | SRR33076656 | SRX28340990 | SRS24670562 | SRP577801 | PRJNA1249431 | DNACas | PRJNA1249431 | Other | Optimization of UV irradiation and PC&PS oligos for gene editing in zebrafish. | DNACas | strain:TAB5|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|age:not applicable|dev stage:24 hpf|collection date:2025 03 12|geo loc name:China|sex:pooled male and female|tissue:whole embryos|BioSampleModel:Model organism or animal | RNA seq of un injected control for fig4 and figsupp10 | Dark3 | Dark3 | adapter | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | PAIRED | ILLUMINA | HiSeq X Ten | SRP577801 | Dark3_R1.fastq.gz Dark3_R2.fastq.gz | fastq fastq | 7177651200.0 | 23925504.0 | Dark3 R1.fastq.gz | 0:150 1:150 | A:1934487156;C:1661154151;G:1670367458;T:1911625892;N:16543 | 150 | 150 | 1934487156 | 1661154151 | 1670367458 | 1911625892 | 16543 | SRX28340990 | SRS24670562 | SRA2110225 | South China Normal University|School of Life Sciences | South China Normal University | B | B | biological fallback assumption | illumina | hiseq_era | unknown | poly_a | unknown | bulk | unknown | unknown | China | 2025-04-11 | Pharyngula | Embryo | Whole Organism | All anatomical structures | ||||||||||||||||||||||||||||||||
| 35858 | 35858 | SRR33076657 | SRX28340989 | SRS24670562 | SRP577801 | PRJNA1249431 | DNACas | PRJNA1249431 | Other | Optimization of UV irradiation and PC&PS oligos for gene editing in zebrafish. | DNACas | strain:TAB5|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|age:not applicable|dev stage:24 hpf|collection date:2025 03 12|geo loc name:China|sex:pooled male and female|tissue:whole embryos|BioSampleModel:Model organism or animal | RNA seq of injected ABEUmax ex2 only for fig4 and figsupp10 | RNP&PCPSUV3 | RNP&PCPSUV3 | adapter | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | PAIRED | ILLUMINA | HiSeq X Ten | SRP577801 | RNP&PCPSUV3_R1.fastq.gz RNP&PCPSUV3_R2.fastq.gz | fastq fastq | 9183109200.0 | 30610364.0 | RNP&PCPSUV3 R1.fastq.gz | 0:150 1:150 | A:2427935909;C:2170838695;G:2180476802;T:2403191776;N:666018 | 150 | 150 | 2427935909 | 2170838695 | 2180476802 | 2403191776 | 666018 | SRX28340989 | SRS24670562 | SRA2110225 | South China Normal University|School of Life Sciences | South China Normal University | B | B | biological fallback assumption | illumina | hiseq_era | unknown | poly_a | unknown | bulk | unknown | unknown | China | 2025-04-11 | Pharyngula | Embryo | Whole Organism | All anatomical structures | ||||||||||||||||||||||||||||||||
| 35859 | 35859 | SRR33076658 | SRX28340988 | SRS24670562 | SRP577801 | PRJNA1249431 | DNACas | PRJNA1249431 | Other | Optimization of UV irradiation and PC&PS oligos for gene editing in zebrafish. | DNACas | strain:TAB5|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|age:not applicable|dev stage:24 hpf|collection date:2025 03 12|geo loc name:China|sex:pooled male and female|tissue:whole embryos|BioSampleModel:Model organism or animal | RNA seq of injected ABEUmax ex2 only for fig4 and figsupp10 | RNP&PCPSUV2 | RNP&PCPSUV2 | adapter | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | PAIRED | ILLUMINA | HiSeq X Ten | SRP577801 | RNP&PCPSUV2_R1.fastq.gz RNP&PCPSUV2_R2.fastq.gz | fastq fastq | 8256027900.0 | 27520093.0 | RNP&PCPSUV2 R1.fastq.gz | 0:150 1:150 | A:2206058533;C:1930339773;G:1937127996;T:2181906664;N:594934 | 150 | 150 | 2206058533 | 1930339773 | 1937127996 | 2181906664 | 594934 | SRX28340988 | SRS24670562 | SRA2110225 | South China Normal University|School of Life Sciences | South China Normal University | B | B | biological fallback assumption | illumina | hiseq_era | unknown | poly_a | unknown | bulk | unknown | unknown | China | 2025-04-11 | Pharyngula | Embryo | Whole Organism | All anatomical structures | ||||||||||||||||||||||||||||||||
| 35860 | 35860 | SRR33076659 | SRX28340987 | SRS24670562 | SRP577801 | PRJNA1249431 | DNACas | PRJNA1249431 | Other | Optimization of UV irradiation and PC&PS oligos for gene editing in zebrafish. | DNACas | strain:TAB5|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|age:not applicable|dev stage:24 hpf|collection date:2025 03 12|geo loc name:China|sex:pooled male and female|tissue:whole embryos|BioSampleModel:Model organism or animal | RNA seq of injected ABEUmax ex2 only for fig4 and figsupp10 | RNP&PCPSUV1 | RNP&PCPSUV1 | adapter | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | PAIRED | ILLUMINA | HiSeq X Ten | SRP577801 | RNP&PCPSUV1_R1.fastq.gz RNP&PCPSUV1_R2.fastq.gz | fastq fastq | 9632672100.0 | 32108907.0 | RNP&PCPSUV1 R1.fastq.gz | 0:150 1:150 | A:2576906321;C:2249405382;G:2259699546;T:2545985362;N:675489 | 150 | 150 | 2576906321 | 2249405382 | 2259699546 | 2545985362 | 675489 | SRX28340987 | SRS24670562 | SRA2110225 | South China Normal University|School of Life Sciences | South China Normal University | B | B | biological fallback assumption | illumina | hiseq_era | unknown | poly_a | unknown | bulk | unknown | unknown | China | 2025-04-11 | Pharyngula | Embryo | Whole Organism | All anatomical structures | ||||||||||||||||||||||||||||||||
| 35861 | 35861 | SRR33076660 | SRX28340986 | SRS24670562 | SRP577801 | PRJNA1249431 | DNACas | PRJNA1249431 | Other | Optimization of UV irradiation and PC&PS oligos for gene editing in zebrafish. | DNACas | strain:TAB5|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|age:not applicable|dev stage:24 hpf|collection date:2025 03 12|geo loc name:China|sex:pooled male and female|tissue:whole embryos|BioSampleModel:Model organism or animal | RNA seq of injected ABEUmax ex1 only for fig4 and figsupp10 | RNP&PCPSdark3 | RNP&PCPSdark3 | adapter | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | PAIRED | ILLUMINA | HiSeq X Ten | SRP577801 | RNP&PCPSdark3_R1.fastq.gz RNP&PCPSdark3_R2.fastq.gz | fastq fastq | 8660039700.0 | 28866799.0 | RNP&PCPSdark3 R1.fastq.gz | 0:150 1:150 | A:2299279682;C:2038230549;G:2048904251;T:2273007920;N:617298 | 150 | 150 | 2299279682 | 2038230549 | 2048904251 | 2273007920 | 617298 | SRX28340986 | SRS24670562 | SRA2110225 | South China Normal University|School of Life Sciences | South China Normal University | B | B | biological fallback assumption | illumina | hiseq_era | unknown | poly_a | unknown | bulk | unknown | unknown | China | 2025-04-11 | Pharyngula | Embryo | Whole Organism | All anatomical structures | ||||||||||||||||||||||||||||||||
| 35862 | 35862 | SRR33076661 | SRX28340985 | SRS24670562 | SRP577801 | PRJNA1249431 | DNACas | PRJNA1249431 | Other | Optimization of UV irradiation and PC&PS oligos for gene editing in zebrafish. | DNACas | strain:TAB5|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|age:not applicable|dev stage:24 hpf|collection date:2025 03 12|geo loc name:China|sex:pooled male and female|tissue:whole embryos|BioSampleModel:Model organism or animal | RNA seq of injected ABEUmax ex1 only for fig4 and figsupp10 | RNP&PCPSdark2 | RNP&PCPSdark2 | adapter | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | PAIRED | ILLUMINA | HiSeq X Ten | SRP577801 | RNP&PCPSdark2_R1.fastq.gz RNP&PCPSdark2_R2.fastq.gz | fastq fastq | 7700900100.0 | 25669667.0 | RNP&PCPSdark2 R1.fastq.gz | 0:150 1:150 | A:2032573331;C:1824438093;G:1835289521;T:2008057350;N:541805 | 150 | 150 | 2032573331 | 1824438093 | 1835289521 | 2008057350 | 541805 | SRX28340985 | SRS24670562 | SRA2110225 | South China Normal University|School of Life Sciences | South China Normal University | B | B | biological fallback assumption | illumina | hiseq_era | unknown | poly_a | unknown | bulk | unknown | unknown | China | 2025-04-11 | Pharyngula | Embryo | Whole Organism | All anatomical structures | ||||||||||||||||||||||||||||||||
| 35863 | 35863 | SRR33076662 | SRX28340984 | SRS24670562 | SRP577801 | PRJNA1249431 | DNACas | PRJNA1249431 | Other | Optimization of UV irradiation and PC&PS oligos for gene editing in zebrafish. | DNACas | strain:TAB5|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|age:not applicable|dev stage:24 hpf|collection date:2025 03 12|geo loc name:China|sex:pooled male and female|tissue:whole embryos|BioSampleModel:Model organism or animal | RNA seq of un injected control for fig4 and figsupp10 | Dark2 | Dark2 | adapter | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | PAIRED | ILLUMINA | HiSeq X Ten | SRP577801 | Dark2_R1.fastq.gz Dark2_R2.fastq.gz | fastq fastq | 6655275600.0 | 22184252.0 | Dark2 R1.fastq.gz | 0:150 1:150 | A:1780712124;C:1553476639;G:1562597740;T:1758469986;N:19111 | 150 | 150 | 1780712124 | 1553476639 | 1562597740 | 1758469986 | 19111 | SRX28340984 | SRS24670562 | SRA2110225 | South China Normal University|School of Life Sciences | South China Normal University | B | B | biological fallback assumption | illumina | hiseq_era | unknown | poly_a | unknown | bulk | unknown | unknown | China | 2025-04-11 | Pharyngula | Embryo | Whole Organism | All anatomical structures | ||||||||||||||||||||||||||||||||
| 35864 | 35864 | SRR33076663 | SRX28340983 | SRS24670562 | SRP577801 | PRJNA1249431 | DNACas | PRJNA1249431 | Other | Optimization of UV irradiation and PC&PS oligos for gene editing in zebrafish. | DNACas | strain:TAB5|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|age:not applicable|dev stage:24 hpf|collection date:2025 03 12|geo loc name:China|sex:pooled male and female|tissue:whole embryos|BioSampleModel:Model organism or animal | RNA seq of un injected control for fig4 and figsupp10 | Dark1 | Dark1 | adapter | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | PAIRED | ILLUMINA | HiSeq X Ten | SRP577801 | Dark1_R1.fastq.gz Dark1_R2.fastq.gz | fastq fastq | 6612663000.0 | 22042210.0 | Dark1 R1.fastq.gz | 0:150 1:150 | A:1769599358;C:1542330464;G:1552982402;T:1747730351;N:20425 | 150 | 150 | 1769599358 | 1542330464 | 1552982402 | 1747730351 | 20425 | SRX28340983 | SRS24670562 | SRA2110225 | South China Normal University|School of Life Sciences | South China Normal University | B | B | biological fallback assumption | illumina | hiseq_era | unknown | poly_a | unknown | bulk | unknown | unknown | China | 2025-04-11 | Pharyngula | Embryo | Whole Organism | All anatomical structures | ||||||||||||||||||||||||||||||||
| 48734 | 48734 | SRR7789588 | SRX4644415 | SRS3742485 | SRP159670 | PRJNA475635 | Tris1 3 dichloro 2 propyl phosphate Exposure During Early Blastula Alters the Normal Trajectory of Zebrafish Embryogenesis | PRJNA475635 | Other | The objective of this project was to rely on mRNA sequencing and amplicon sequencing to test the hypothesis that tris1 3 dichloro 2 propyl phosphate TDCIPP interferes with pathways involved in early zebrafish embryogenesis. | TDCIPP 312uM 24hpf 1 S7 | strain:5D|isolate:88|dev stage:24 hpf|sex:not applicable|tissue:embryo|BioSampleModel:Model organism or animal | TDCIPP 312uM 24hpf 1 S7 | TDCIPP 312uM 24hpf 1 S7 | TDCIPP 312uM 24hpf 1 S7 | QuantSeq three prime mRNA Seq Library Prep Kit FWD | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina MiniSeq | SRP159670 | TDCIPP-312uM-24hpf-1_S7_L001_R1_001.fastq.gz | fastq | 184588031.0 | 2462646.0 | TDCIPP 312uM 24hpf 1 S7 L001 R1 001.fastq.gz | 0:74.96 1:0 | A:59836053;C:33139428;G:43400445;T:48078483;N:133622 | 74 | 0 | 59836053 | 33139428 | 43400445 | 48078483 | 133622 | SRX4644415 | SRS3742485 | SRA767209 | University of California, Riverside|Environmental Sciences | University of California, Riverside | 1 | 0.79726 | 0.1642 | 0.7933 | 0.5999 | 76 | B | usable mapping rate | illumina | miseq | 3prime | poly_a | lexogen | bulk | unknown | unknown | United States | 2018-09-05 | Pharyngula | Embryo | Embryo Imprecise | All anatomical structures | |||||||||||||||||||||||||||
| 48735 | 48735 | SRR7789589 | SRX4644414 | SRS3742484 | SRP159670 | PRJNA475635 | Tris1 3 dichloro 2 propyl phosphate Exposure During Early Blastula Alters the Normal Trajectory of Zebrafish Embryogenesis | PRJNA475635 | Other | The objective of this project was to rely on mRNA sequencing and amplicon sequencing to test the hypothesis that tris1 3 dichloro 2 propyl phosphate TDCIPP interferes with pathways involved in early zebrafish embryogenesis. | TDCIPP 156uM 24hpf 3 S9 | strain:5D|isolate:87|dev stage:24 hpf|sex:not applicable|tissue:embryo|BioSampleModel:Model organism or animal | TDCIPP 156uM 24hpf 3 S9 | TDCIPP 156uM 24hpf 3 S9 | TDCIPP 156uM 24hpf 3 S9 | QuantSeq three prime mRNA Seq Library Prep Kit FWD | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina MiniSeq | SRP159670 | TDCIPP-156uM-24hpf-3_S9_L001_R1_001.fastq.gz | fastq | 184876248.0 | 2453513.0 | TDCIPP 156uM 24hpf 3 S9 L001 R1 001.fastq.gz | 0:75.35 1:0 | A:53410957;C:33148221;G:44687015;T:53588493;N:41562 | 75 | 0 | 53410957 | 33148221 | 44687015 | 53588493 | 41562 | SRX4644414 | SRS3742484 | SRA767209 | University of California, Riverside|Environmental Sciences | University of California, Riverside | 1 | 0.87915 | 0.16605 | 0.77244 | 0.5773 | 76 | B | usable mapping rate | illumina | miseq | 3prime | poly_a | lexogen | bulk | unknown | unknown | United States | 2018-09-05 | Pharyngula | Embryo | Embryo Imprecise | All anatomical structures | |||||||||||||||||||||||||||
| 48736 | 48736 | SRR7789590 | SRX4644413 | SRS3742483 | SRP159670 | PRJNA475635 | Tris1 3 dichloro 2 propyl phosphate Exposure During Early Blastula Alters the Normal Trajectory of Zebrafish Embryogenesis | PRJNA475635 | Other | The objective of this project was to rely on mRNA sequencing and amplicon sequencing to test the hypothesis that tris1 3 dichloro 2 propyl phosphate TDCIPP interferes with pathways involved in early zebrafish embryogenesis. | TDCIPP 156uM 24hpf 2 S8 | strain:5D|isolate:86|dev stage:24 hpf|sex:not applicable|tissue:embryo|BioSampleModel:Model organism or animal | TDCIPP 156uM 24hpf 2 S8 | TDCIPP 156uM 24hpf 2 S8 | TDCIPP 156uM 24hpf 2 S8 | QuantSeq three prime mRNA Seq Library Prep Kit FWD | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina MiniSeq | SRP159670 | TDCIPP-156uM-24hpf-2_S8_L001_R1_001.fastq.gz | fastq | 140080067.0 | 1862101.0 | TDCIPP 156uM 24hpf 2 S8 L001 R1 001.fastq.gz | 0:75.23 1:0 | A:42438761;C:24980897;G:33224272;T:39385900;N:50237 | 75 | 0 | 42438761 | 24980897 | 33224272 | 39385900 | 50237 | SRX4644413 | SRS3742483 | SRA767209 | University of California, Riverside|Environmental Sciences | University of California, Riverside | 1 | 0.85826 | 0.14847 | 0.77916 | 0.57916 | 73 | B | usable mapping rate | illumina | miseq | 3prime | poly_a | lexogen | bulk | unknown | unknown | United States | 2018-09-05 | Pharyngula | Embryo | Embryo Imprecise | All anatomical structures | |||||||||||||||||||||||||||
| 48737 | 48737 | SRR7789591 | SRX4644412 | SRS3742482 | SRP159670 | PRJNA475635 | Tris1 3 dichloro 2 propyl phosphate Exposure During Early Blastula Alters the Normal Trajectory of Zebrafish Embryogenesis | PRJNA475635 | Other | The objective of this project was to rely on mRNA sequencing and amplicon sequencing to test the hypothesis that tris1 3 dichloro 2 propyl phosphate TDCIPP interferes with pathways involved in early zebrafish embryogenesis. | TDCIPP 156uM 24hpf 1 S12 | strain:5D|isolate:85|dev stage:24 hpf|sex:not applicable|tissue:embryo|BioSampleModel:Model organism or animal | TDCIPP 156uM 24hpf 1 S12 | TDCIPP 156uM 24hpf 1 S12 | TDCIPP 156uM 24hpf 1 S12 | QuantSeq three prime mRNA Seq Library Prep Kit FWD | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina MiniSeq | SRP159670 | TDCIPP-156uM-24hpf-1_S12_L001_R1_001.fastq.gz | fastq | 218911092.0 | 2910723.0 | TDCIPP 156uM 24hpf 1 S12 L001 R1 001.fastq.gz | 0:75.21 1:0 | A:66938762;C:38751166;G:50479676;T:62654864;N:86624 | 75 | 0 | 66938762 | 38751166 | 50479676 | 62654864 | 86624 | SRX4644412 | SRS3742482 | SRA767209 | University of California, Riverside|Environmental Sciences | University of California, Riverside | 1 | 0.84868 | 0.15475 | 0.77715 | 0.57656 | 76 | B | usable mapping rate | illumina | miseq | 3prime | poly_a | lexogen | bulk | unknown | unknown | United States | 2018-09-05 | Pharyngula | Embryo | Embryo Imprecise | All anatomical structures | |||||||||||||||||||||||||||
| 48738 | 48738 | SRR7789592 | SRX4644411 | SRS3742481 | SRP159670 | PRJNA475635 | Tris1 3 dichloro 2 propyl phosphate Exposure During Early Blastula Alters the Normal Trajectory of Zebrafish Embryogenesis | PRJNA475635 | Other | The objective of this project was to rely on mRNA sequencing and amplicon sequencing to test the hypothesis that tris1 3 dichloro 2 propyl phosphate TDCIPP interferes with pathways involved in early zebrafish embryogenesis. | DorsoM 24hpf 3 S6 | strain:5D|isolate:84|dev stage:24 hpf|sex:not applicable|tissue:embryo|BioSampleModel:Model organism or animal | DorsoM 24hpf 3 S6 | DorsoM 24hpf 3 S6 | DorsoM 24hpf 3 S6 | QuantSeq three prime mRNA Seq Library Prep Kit FWD | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina MiniSeq | SRP159670 | DorsoM-24hpf-3_S6_L001_R1_001.fastq.gz | fastq | 226197307.0 | 3007869.0 | DorsoM 24hpf 3 S6 L001 R1 001.fastq.gz | 0:75.20 1:0 | A:69014123;C:40615780;G:53395820;T:63087228;N:84356 | 75 | 0 | 69014123 | 40615780 | 53395820 | 63087228 | 84356 | SRX4644411 | SRS3742481 | SRA767209 | University of California, Riverside|Environmental Sciences | University of California, Riverside | 1 | 0.84411 | 0.16582 | 0.78409 | 0.58746 | 75 | B | usable mapping rate | illumina | miseq | 3prime | poly_a | lexogen | bulk | unknown | unknown | United States | 2018-09-05 | Pharyngula | Embryo | Embryo Imprecise | All anatomical structures | |||||||||||||||||||||||||||
| 48739 | 48739 | SRR7789593 | SRX4644410 | SRS3742480 | SRP159670 | PRJNA475635 | Tris1 3 dichloro 2 propyl phosphate Exposure During Early Blastula Alters the Normal Trajectory of Zebrafish Embryogenesis | PRJNA475635 | Other | The objective of this project was to rely on mRNA sequencing and amplicon sequencing to test the hypothesis that tris1 3 dichloro 2 propyl phosphate TDCIPP interferes with pathways involved in early zebrafish embryogenesis. | DorsoM 24hpf 2 S4 | strain:5D|isolate:83|dev stage:24 hpf|sex:not applicable|tissue:embryo|BioSampleModel:Model organism or animal | DorsoM 24hpf 2 S4 | DorsoM 24hpf 2 S4 | DorsoM 24hpf 2 S4 | QuantSeq three prime mRNA Seq Library Prep Kit FWD | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina MiniSeq | SRP159670 | DorsoM-24hpf-2_S4_L001_R1_001.fastq.gz | fastq | 200787104.0 | 2671998.0 | DorsoM 24hpf 2 S4 L001 R1 001.fastq.gz | 0:75.14 1:0 | A:62684374;C:36326611;G:48099771;T:53588669;N:87679 | 75 | 0 | 62684374 | 36326611 | 48099771 | 53588669 | 87679 | SRX4644410 | SRS3742480 | SRA767209 | University of California, Riverside|Environmental Sciences | University of California, Riverside | 1 | 0.83445 | 0.17548 | 0.78975 | 0.59538 | 76 | B | usable mapping rate | illumina | miseq | 3prime | poly_a | lexogen | bulk | unknown | unknown | United States | 2018-09-05 | Pharyngula | Embryo | Embryo Imprecise | All anatomical structures | |||||||||||||||||||||||||||
| 48740 | 48740 | SRR7789594 | SRX4644409 | SRS3742479 | SRP159670 | PRJNA475635 | Tris1 3 dichloro 2 propyl phosphate Exposure During Early Blastula Alters the Normal Trajectory of Zebrafish Embryogenesis | PRJNA475635 | Other | The objective of this project was to rely on mRNA sequencing and amplicon sequencing to test the hypothesis that tris1 3 dichloro 2 propyl phosphate TDCIPP interferes with pathways involved in early zebrafish embryogenesis. | DorsoM 24hpf 1 S5 | strain:5D|isolate:82|dev stage:24 hpf|sex:not applicable|tissue:embryo|BioSampleModel:Model organism or animal | DorsoM 24hpf 1 S5 | DorsoM 24hpf 1 S5 | DorsoM 24hpf 1 S5 | QuantSeq three prime mRNA Seq Library Prep Kit FWD | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina MiniSeq | SRP159670 | DorsoM-24hpf-1_S5_L001_R1_001.fastq.gz | fastq | 206040580.0 | 2740232.0 | DorsoM 24hpf 1 S5 L001 R1 001.fastq.gz | 0:75.19 1:0 | A:63205154;C:36811449;G:49166561;T:56767117;N:90299 | 75 | 0 | 63205154 | 36811449 | 49166561 | 56767117 | 90299 | SRX4644409 | SRS3742479 | SRA767209 | University of California, Riverside|Environmental Sciences | University of California, Riverside | 1 | 0.8485 | 0.16417 | 0.78447 | 0.591 | 74 | B | usable mapping rate | illumina | miseq | 3prime | poly_a | lexogen | bulk | unknown | unknown | United States | 2018-09-05 | Pharyngula | Embryo | Embryo Imprecise | All anatomical structures | |||||||||||||||||||||||||||
| 48741 | 48741 | SRR7789595 | SRX4644408 | SRS3742478 | SRP159670 | PRJNA475635 | Tris1 3 dichloro 2 propyl phosphate Exposure During Early Blastula Alters the Normal Trajectory of Zebrafish Embryogenesis | PRJNA475635 | Other | The objective of this project was to rely on mRNA sequencing and amplicon sequencing to test the hypothesis that tris1 3 dichloro 2 propyl phosphate TDCIPP interferes with pathways involved in early zebrafish embryogenesis. | DMSO 24hpf 3 S1 | strain:5D|isolate:81|dev stage:24 hpf|sex:not applicable|tissue:embryo|BioSampleModel:Model organism or animal | DMSO 24hpf 3 S1 | DMSO 24hpf 3 S1 | DMSO 24hpf 3 S1 | QuantSeq three prime mRNA Seq Library Prep Kit FWD | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina MiniSeq | SRP159670 | DMSO-24hpf-3_S1_L001_R1_001.fastq.gz | fastq | 200487551.0 | 2662001.0 | DMSO 24hpf 3 S1 L001 R1 001.fastq.gz | 0:75.31 1:0 | A:58320856;C:36521443;G:49072076;T:56528109;N:45067 | 75 | 0 | 58320856 | 36521443 | 49072076 | 56528109 | 45067 | SRX4644408 | SRS3742478 | SRA767209 | University of California, Riverside|Environmental Sciences | University of California, Riverside | 1 | 0.87328 | 0.15746 | 0.78165 | 0.57581 | 76 | B | usable mapping rate | illumina | miseq | 3prime | poly_a | lexogen | bulk | unknown | unknown | United States | 2018-09-05 | Pharyngula | Embryo | Embryo Imprecise | All anatomical structures | |||||||||||||||||||||||||||
| 48750 | 48750 | SRR7789604 | SRX4644399 | SRS3742469 | SRP159670 | PRJNA475635 | Tris1 3 dichloro 2 propyl phosphate Exposure During Early Blastula Alters the Normal Trajectory of Zebrafish Embryogenesis | PRJNA475635 | Other | The objective of this project was to rely on mRNA sequencing and amplicon sequencing to test the hypothesis that tris1 3 dichloro 2 propyl phosphate TDCIPP interferes with pathways involved in early zebrafish embryogenesis. | DMSO 24hpf 1 S3 | strain:5D|isolate:79|dev stage:24 hpf|sex:not applicable|tissue:embryo|BioSampleModel:Model organism or animal | DMSO 24hpf 1 S3 | DMSO 24hpf 1 S3 | DMSO 24hpf 1 S3 | QuantSeq three prime mRNA Seq Library Prep Kit FWD | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina MiniSeq | SRP159670 | DMSO-24hpf-1_S3_L001_R1_001.fastq.gz | fastq | 195559158.0 | 2597033.0 | DMSO 24hpf 1 S3 L001 R1 001.fastq.gz | 0:75.30 1:0 | A:57417392;C:35134927;G:47296304;T:55662008;N:48527 | 75 | 0 | 57417392 | 35134927 | 47296304 | 55662008 | 48527 | SRX4644399 | SRS3742469 | SRA767209 | University of California, Riverside|Environmental Sciences | University of California, Riverside | 1 | 0.86713 | 0.17324 | 0.77926 | 0.57305 | 76 | B | usable mapping rate | illumina | miseq | 3prime | poly_a | lexogen | bulk | unknown | unknown | United States | 2018-09-05 | Pharyngula | Embryo | Embryo Imprecise | All anatomical structures | |||||||||||||||||||||||||||
| 48751 | 48751 | SRR7789605 | SRX4644398 | SRS3742468 | SRP159670 | PRJNA475635 | Tris1 3 dichloro 2 propyl phosphate Exposure During Early Blastula Alters the Normal Trajectory of Zebrafish Embryogenesis | PRJNA475635 | Other | The objective of this project was to rely on mRNA sequencing and amplicon sequencing to test the hypothesis that tris1 3 dichloro 2 propyl phosphate TDCIPP interferes with pathways involved in early zebrafish embryogenesis. | DMSO 24hpf 2 S2 | strain:5D|isolate:80|dev stage:24 hpf|sex:not applicable|tissue:embryo|BioSampleModel:Model organism or animal | DMSO 24hpf 2 S2 | DMSO 24hpf 2 S2 | DMSO 24hpf 2 S2 | QuantSeq three prime mRNA Seq Library Prep Kit FWD | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina MiniSeq | SRP159670 | DMSO-24hpf-2_S2_L001_R1_001.fastq.gz | fastq | 166572474.0 | 2212698.0 | DMSO 24hpf 2 S2 L001 R1 001.fastq.gz | 0:75.28 1:0 | A:48640696;C:30601449;G:40273907;T:47002888;N:53534 | 75 | 0 | 48640696 | 30601449 | 40273907 | 47002888 | 53534 | SRX4644398 | SRS3742468 | SRA767209 | University of California, Riverside|Environmental Sciences | University of California, Riverside | 1 | 0.87345 | 0.17124 | 0.77942 | 0.56446 | 76 | B | usable mapping rate | illumina | miseq | 3prime | poly_a | lexogen | bulk | unknown | unknown | United States | 2018-09-05 | Pharyngula | Embryo | Embryo Imprecise | All anatomical structures | |||||||||||||||||||||||||||
| 48762 | 48762 | SRR7789616 | SRX4644387 | SRS3742457 | SRP159670 | PRJNA475635 | Tris1 3 dichloro 2 propyl phosphate Exposure During Early Blastula Alters the Normal Trajectory of Zebrafish Embryogenesis | PRJNA475635 | Other | The objective of this project was to rely on mRNA sequencing and amplicon sequencing to test the hypothesis that tris1 3 dichloro 2 propyl phosphate TDCIPP interferes with pathways involved in early zebrafish embryogenesis. | TDCIPP 312uM 24hpf 3 S10 | strain:5D|isolate:90|dev stage:24 hpf|sex:not applicable|tissue:embryo|BioSampleModel:Model organism or animal | TDCIPP 312uM 24hpf 3 S10 | TDCIPP 312uM 24hpf 3 S10 | TDCIPP 312uM 24hpf 3 S10 | QuantSeq three prime mRNA Seq Library Prep Kit FWD | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina MiniSeq | SRP159670 | TDCIPP-312uM-24hpf-3_S10_L001_R1_001.fastq.gz | fastq | 189680978.0 | 2521266.0 | TDCIPP 312uM 24hpf 3 S10 L001 R1 001.fastq.gz | 0:75.23 1:0 | A:57371176;C:34308154;G:45128853;T:52806417;N:66378 | 75 | 0 | 57371176 | 34308154 | 45128853 | 52806417 | 66378 | SRX4644387 | SRS3742457 | SRA767209 | University of California, Riverside|Environmental Sciences | University of California, Riverside | 1 | 0.8537 | 0.15223 | 0.7875 | 0.60037 | 76 | B | usable mapping rate | illumina | miseq | 3prime | poly_a | lexogen | bulk | unknown | unknown | United States | 2018-09-05 | Pharyngula | Embryo | Embryo Imprecise | All anatomical structures | |||||||||||||||||||||||||||
| 48763 | 48763 | SRR7789617 | SRX4644386 | SRS3742456 | SRP159670 | PRJNA475635 | Tris1 3 dichloro 2 propyl phosphate Exposure During Early Blastula Alters the Normal Trajectory of Zebrafish Embryogenesis | PRJNA475635 | Other | The objective of this project was to rely on mRNA sequencing and amplicon sequencing to test the hypothesis that tris1 3 dichloro 2 propyl phosphate TDCIPP interferes with pathways involved in early zebrafish embryogenesis. | TDCIPP 312uM 24hpf 2 S11 | strain:5D|isolate:89|dev stage:24 hpf|sex:not applicable|tissue:embryo|BioSampleModel:Model organism or animal | TDCIPP 312uM 24hpf 2 S11 | TDCIPP 312uM 24hpf 2 S11 | TDCIPP 312uM 24hpf 2 S11 | QuantSeq three prime mRNA Seq Library Prep Kit FWD | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina MiniSeq | SRP159670 | TDCIPP-312uM-24hpf-2_S11_L001_R1_001.fastq.gz | fastq | 187372726.0 | 2491862.0 | TDCIPP 312uM 24hpf 2 S11 L001 R1 001.fastq.gz | 0:75.19 1:0 | A:56398578;C:34043199;G:44675648;T:52187191;N:68110 | 75 | 0 | 56398578 | 34043199 | 44675648 | 52187191 | 68110 | SRX4644386 | SRS3742456 | SRA767209 | University of California, Riverside|Environmental Sciences | University of California, Riverside | 1 | 0.8514 | 0.1782 | 0.79166 | 0.59381 | 73 | B | usable mapping rate | illumina | miseq | 3prime | poly_a | lexogen | bulk | unknown | unknown | United States | 2018-09-05 | Pharyngula | Embryo | Embryo Imprecise | All anatomical structures | |||||||||||||||||||||||||||
| 50887 | 50887 | SRR8784481 | SRX5574334 | SRS4536892 | SRP189394 | PRJNA511154 | Diphenyl Phosphate Induced Toxicity During Embryonic Development | PRJNA511154 | Other | The objective of this project was to rely on mRNA sequencing to determine the potential impacts of diphenyl phosphate DPHP on the embryonic transcriptome in zebrafish. | DPHP D 30 1 2 S10 | strain:5D|isolate:2|dev stage:30 hpf|sex:not applicable|tissue:embryo|BioSampleModel:Model organism or animal | DPHP D 30 1 2 S10 | DPHP D 30 1 2 S10 | DPHP D 30 1 2 S10 | QuantSeq three prime mRNA Seq Library Prep Kit FWD | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina MiniSeq | SRP189394 | D-30-1-2_S10_L001_R1_001.fastq.gz | fastq | 238461832.0 | 3201052.0 | D 30 1 2 S10 L001 R1 001.fastq.gz | 0:74.49 1:0 | A:79390298;C:43261018;G:59486784;T:54551984;N:1771748 | 74 | 0 | 79390298 | 43261018 | 59486784 | 54551984 | 1771748 | SRX5574334 | SRS4536892 | SRA865872 | University of California, Riverside|Environmental Sciences | University of California, Riverside | 1 | 0.69702 | 0.19078 | 0.81546 | 0.53635 | 76 | B | usable mapping rate | illumina | miseq | 3prime | poly_a | lexogen | bulk | unknown | unknown | United States | 2019-03-25 | Pharyngula | Embryo | Embryo Imprecise | All anatomical structures | |||||||||||||||||||||||||||
| 50888 | 50888 | SRR8784482 | SRX5574333 | SRS4536891 | SRP189394 | PRJNA511154 | Diphenyl Phosphate Induced Toxicity During Embryonic Development | PRJNA511154 | Other | The objective of this project was to rely on mRNA sequencing to determine the potential impacts of diphenyl phosphate DPHP on the embryonic transcriptome in zebrafish. | DPHP D 30 1 S12 | strain:5D|isolate:1|dev stage:30 hpf|sex:not applicable|tissue:embryo|BioSampleModel:Model organism or animal | DPHP D 30 1 S12 | DPHP D 30 1 S12 | DPHP D 30 1 S12 | QuantSeq three prime mRNA Seq Library Prep Kit FWD | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina MiniSeq | SRP189394 | D-30-1_S12_L001_R1_001.fastq.gz | fastq | 127484541.0 | 1705353.0 | D 30 1 S12 L001 R1 001.fastq.gz | 0:74.76 1:0 | A:41547496;C:23209432;G:33043143;T:28929047;N:755423 | 74 | 0 | 41547496 | 23209432 | 33043143 | 28929047 | 755423 | SRX5574333 | SRS4536891 | SRA865872 | University of California, Riverside|Environmental Sciences | University of California, Riverside | 1 | 0.70027 | 0.2065 | 0.81326 | 0.52147 | 76 | B | usable mapping rate | illumina | miseq | 3prime | poly_a | lexogen | bulk | unknown | unknown | United States | 2019-03-25 | Pharyngula | Embryo | Embryo Imprecise | All anatomical structures | |||||||||||||||||||||||||||
| 50889 | 50889 | SRR8784483 | SRX5574332 | SRS4536890 | SRP189394 | PRJNA511154 | Diphenyl Phosphate Induced Toxicity During Embryonic Development | PRJNA511154 | Other | The objective of this project was to rely on mRNA sequencing to determine the potential impacts of diphenyl phosphate DPHP on the embryonic transcriptome in zebrafish. | DPHP D 30 2 2 S12 | strain:5D|isolate:4|dev stage:30 hpf|sex:not applicable|tissue:embryo|BioSampleModel:Model organism or animal | DPHP D 30 2 2 S12 | DPHP D 30 2 2 S12 | DPHP D 30 2 2 S12 | QuantSeq three prime mRNA Seq Library Prep Kit FWD | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina MiniSeq | SRP189394 | D-30-2-2_S12_L001_R1_001.fastq.gz | fastq | 158402653.0 | 2124088.0 | D 30 2 2 S12 L001 R1 001.fastq.gz | 0:74.57 1:0 | A:52165100;C:29584526;G:39654761;T:35999190;N:999076 | 74 | 0 | 52165100 | 29584526 | 39654761 | 35999190 | 999076 | SRX5574332 | SRS4536890 | SRA865872 | University of California, Riverside|Environmental Sciences | University of California, Riverside | 1 | 0.70228 | 0.19977 | 0.81505 | 0.52556 | 76 | B | usable mapping rate | illumina | miseq | 3prime | poly_a | lexogen | bulk | unknown | unknown | United States | 2019-03-25 | Pharyngula | Embryo | Embryo Imprecise | All anatomical structures | |||||||||||||||||||||||||||
| 50890 | 50890 | SRR8784484 | SRX5574331 | SRS4536889 | SRP189394 | PRJNA511154 | Diphenyl Phosphate Induced Toxicity During Embryonic Development | PRJNA511154 | Other | The objective of this project was to rely on mRNA sequencing to determine the potential impacts of diphenyl phosphate DPHP on the embryonic transcriptome in zebrafish. | DPHP D 30 2 S11 | strain:5D|isolate:3|dev stage:30 hpf|sex:not applicable|tissue:embryo|BioSampleModel:Model organism or animal | DPHP D 30 2 S11 | DPHP D 30 2 S11 | DPHP D 30 2 S11 | QuantSeq three prime mRNA Seq Library Prep Kit FWD | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina MiniSeq | SRP189394 | D-30-2_S11_L001_R1_001.fastq.gz | fastq | 181133063.0 | 2424959.0 | D 30 2 S11 L001 R1 001.fastq.gz | 0:74.70 1:0 | A:59538982;C:31950249;G:46981647;T:41411578;N:1250607 | 74 | 0 | 59538982 | 31950249 | 46981647 | 41411578 | 1250607 | SRX5574331 | SRS4536889 | SRA865872 | University of California, Riverside|Environmental Sciences | University of California, Riverside | 1 | 0.69274 | 0.19587 | 0.81199 | 0.53253 | 76 | B | usable mapping rate | illumina | miseq | 3prime | poly_a | lexogen | bulk | unknown | unknown | United States | 2019-03-25 | Pharyngula | Embryo | Embryo Imprecise | All anatomical structures | |||||||||||||||||||||||||||
| 50891 | 50891 | SRR8784485 | SRX5574330 | SRS4536888 | SRP189394 | PRJNA511154 | Diphenyl Phosphate Induced Toxicity During Embryonic Development | PRJNA511154 | Other | The objective of this project was to rely on mRNA sequencing to determine the potential impacts of diphenyl phosphate DPHP on the embryonic transcriptome in zebrafish. | DPHP D 30 3 2 S8 | strain:5D|isolate:6|dev stage:30 hpf|sex:not applicable|tissue:embryo|BioSampleModel:Model organism or animal | DPHP D 30 3 2 S8 | DPHP D 30 3 2 S8 | DPHP D 30 3 2 S8 | QuantSeq three prime mRNA Seq Library Prep Kit FWD | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina MiniSeq | SRP189394 | D-30-3-2_S8_L001_R1_001.fastq.gz | fastq | 144659077.0 | 1979750.0 | D 30 3 2 S8 L001 R1 001.fastq.gz | 0:73.07 1:0 | A:45008994;C:26259119;G:34182870;T:35418284;N:3789810 | 73 | 0 | 45008994 | 26259119 | 34182870 | 35418284 | 3789810 | SRX5574330 | SRS4536888 | SRA865872 | University of California, Riverside|Environmental Sciences | University of California, Riverside | 1 | 0.76343 | 0.19294 | 0.79981 | 0.52722 | 73 | B | usable mapping rate | illumina | miseq | 3prime | poly_a | lexogen | bulk | unknown | unknown | United States | 2019-03-25 | Pharyngula | Embryo | Embryo Imprecise | All anatomical structures | |||||||||||||||||||||||||||
| 50892 | 50892 | SRR8784486 | SRX5574329 | SRS4536887 | SRP189394 | PRJNA511154 | Diphenyl Phosphate Induced Toxicity During Embryonic Development | PRJNA511154 | Other | The objective of this project was to rely on mRNA sequencing to determine the potential impacts of diphenyl phosphate DPHP on the embryonic transcriptome in zebrafish. | DPHP D 30 3 S10 | strain:5D|isolate:5|dev stage:30 hpf|sex:not applicable|tissue:embryo|BioSampleModel:Model organism or animal | DPHP D 30 3 S10 | DPHP D 30 3 S10 | DPHP D 30 3 S10 | QuantSeq three prime mRNA Seq Library Prep Kit FWD | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina MiniSeq | SRP189394 | D-30-3_S10_L001_R1_001.fastq.gz | fastq | 208642460.0 | 2849099.0 | D 30 3 S10 L001 R1 001.fastq.gz | 0:73.23 1:0 | A:64082607;C:37314617;G:50698008;T:51281410;N:5265818 | 73 | 0 | 64082607 | 37314617 | 50698008 | 51281410 | 5265818 | SRX5574329 | SRS4536887 | SRA865872 | University of California, Riverside|Environmental Sciences | University of California, Riverside | 1 | 0.76279 | 0.20019 | 0.79703 | 0.53182 | 76 | B | usable mapping rate | illumina | miseq | 3prime | poly_a | lexogen | bulk | unknown | unknown | United States | 2019-03-25 | Pharyngula | Embryo | Embryo Imprecise | All anatomical structures | |||||||||||||||||||||||||||
| 50899 | 50899 | SRR8784493 | SRX5574322 | SRS4536880 | SRP189394 | PRJNA511154 | Diphenyl Phosphate Induced Toxicity During Embryonic Development | PRJNA511154 | Other | The objective of this project was to rely on mRNA sequencing to determine the potential impacts of diphenyl phosphate DPHP on the embryonic transcriptome in zebrafish. | Control F 30 1 2 S5 | strain:5D|isolate:14|dev stage:30 hpf|sex:not applicable|tissue:embryo|BioSampleModel:Model organism or animal | Control F 30 1 2 S5 | Control F 30 1 2 S5 | Control F 30 1 2 S5 | QuantSeq three prime mRNA Seq Library Prep Kit FWD | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina MiniSeq | SRP189394 | F-30-1-2_S5_L001_R1_001.fastq.gz | fastq | 184025156.0 | 2471206.0 | F 30 1 2 S5 L001 R1 001.fastq.gz | 0:74.47 1:0 | A:59460329;C:34704834;G:46210350;T:42162468;N:1487175 | 74 | 0 | 59460329 | 34704834 | 46210350 | 42162468 | 1487175 | SRX5574322 | SRS4536880 | SRA865872 | University of California, Riverside|Environmental Sciences | University of California, Riverside | 1 | 0.71982 | 0.20558 | 0.81422 | 0.54265 | 76 | B | usable mapping rate | illumina | miseq | 3prime | poly_a | lexogen | bulk | unknown | unknown | United States | 2019-03-25 | Pharyngula | Embryo | Embryo Imprecise | All anatomical structures | |||||||||||||||||||||||||||
| 50900 | 50900 | SRR8784494 | SRX5574321 | SRS4536879 | SRP189394 | PRJNA511154 | Diphenyl Phosphate Induced Toxicity During Embryonic Development | PRJNA511154 | Other | The objective of this project was to rely on mRNA sequencing to determine the potential impacts of diphenyl phosphate DPHP on the embryonic transcriptome in zebrafish. | Control F 30 1 S6 | strain:5D|isolate:13|dev stage:30 hpf|sex:not applicable|tissue:embryo|BioSampleModel:Model organism or animal | Control F 30 1 S6 | Control F 30 1 S6 | Control F 30 1 S6 | QuantSeq three prime mRNA Seq Library Prep Kit FWD | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina MiniSeq | SRP189394 | F-30-1_S6_L001_R1_001.fastq.gz | fastq | 167461374.0 | 2244011.0 | F 30 1 S6 L001 R1 001.fastq.gz | 0:74.63 1:0 | A:53497635;C:30881557;G:43359231;T:38396711;N:1326240 | 74 | 0 | 53497635 | 30881557 | 43359231 | 38396711 | 1326240 | SRX5574321 | SRS4536879 | SRA865872 | University of California, Riverside|Environmental Sciences | University of California, Riverside | 1 | 0.71657 | 0.21056 | 0.81347 | 0.53534 | 75 | B | usable mapping rate | illumina | miseq | 3prime | poly_a | lexogen | bulk | unknown | unknown | United States | 2019-03-25 | Pharyngula | Embryo | Embryo Imprecise | All anatomical structures | |||||||||||||||||||||||||||
| 50903 | 50903 | SRR8784497 | SRX5574318 | SRS4536876 | SRP189394 | PRJNA511154 | Diphenyl Phosphate Induced Toxicity During Embryonic Development | PRJNA511154 | Other | The objective of this project was to rely on mRNA sequencing to determine the potential impacts of diphenyl phosphate DPHP on the embryonic transcriptome in zebrafish. | Control F 30 3 2 S1 | strain:5D|isolate:18|dev stage:30 hpf|sex:not applicable|tissue:embryo|BioSampleModel:Model organism or animal | Control F 30 3 2 S1 | Control F 30 3 2 S1 | Control F 30 3 2 S1 | QuantSeq three prime mRNA Seq Library Prep Kit FWD | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina MiniSeq | SRP189394 | F-30-3-2_S1_L001_R1_001.fastq.gz | fastq | 150754933.0 | 2005707.0 | F 30 3 2 S1 L001 R1 001.fastq.gz | 0:75.16 1:0 | A:48638635;C:27543427;G:36117234;T:38241792;N:213845 | 75 | 0 | 48638635 | 27543427 | 36117234 | 38241792 | 213845 | SRX5574318 | SRS4536876 | SRA865872 | University of California, Riverside|Environmental Sciences | University of California, Riverside | 1 | 0.76888 | 0.19109 | 0.79914 | 0.55487 | 76 | B | usable mapping rate | illumina | miseq | 3prime | poly_a | lexogen | bulk | unknown | unknown | United States | 2019-03-25 | Pharyngula | Embryo | Embryo Imprecise | All anatomical structures | |||||||||||||||||||||||||||
| 50904 | 50904 | SRR8784498 | SRX5574317 | SRS4536875 | SRP189394 | PRJNA511154 | Diphenyl Phosphate Induced Toxicity During Embryonic Development | PRJNA511154 | Other | The objective of this project was to rely on mRNA sequencing to determine the potential impacts of diphenyl phosphate DPHP on the embryonic transcriptome in zebrafish. | Control F 30 3 S4 | strain:5D|isolate:17|dev stage:30 hpf|sex:not applicable|tissue:embryo|BioSampleModel:Model organism or animal | Control F 30 3 S4 | Control F 30 3 S4 | Control F 30 3 S4 | QuantSeq three prime mRNA Seq Library Prep Kit FWD | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina MiniSeq | SRP189394 | F-30-3_S4_L001_R1_001.fastq.gz | fastq | 136108968.0 | 1808392.0 | F 30 3 S4 L001 R1 001.fastq.gz | 0:75.27 1:0 | A:43464242;C:24432302;G:33489525;T:34557393;N:165506 | 75 | 0 | 43464242 | 24432302 | 33489525 | 34557393 | 165506 | SRX5574317 | SRS4536875 | SRA865872 | University of California, Riverside|Environmental Sciences | University of California, Riverside | 1 | 0.76602 | 0.19243 | 0.79437 | 0.55662 | 75 | B | usable mapping rate | illumina | miseq | 3prime | poly_a | lexogen | bulk | unknown | unknown | United States | 2019-03-25 | Pharyngula | Embryo | Embryo Imprecise | All anatomical structures | |||||||||||||||||||||||||||
| 50905 | 50905 | SRR8784499 | SRX5574316 | SRS4536874 | SRP189394 | PRJNA511154 | Diphenyl Phosphate Induced Toxicity During Embryonic Development | PRJNA511154 | Other | The objective of this project was to rely on mRNA sequencing to determine the potential impacts of diphenyl phosphate DPHP on the embryonic transcriptome in zebrafish. | Control F 30 2 2 S2 | strain:5D|isolate:16|dev stage:30 hpf|sex:not applicable|tissue:embryo|BioSampleModel:Model organism or animal | Control F 30 2 2 S2 | Control F 30 2 2 S2 | Control F 30 2 2 S2 | QuantSeq three prime mRNA Seq Library Prep Kit FWD | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina MiniSeq | SRP189394 | F-30-2-2_S2_L001_R1_001.fastq.gz | fastq | 136980240.0 | 1822208.0 | F 30 2 2 S2 L001 R1 001.fastq.gz | 0:75.17 1:0 | A:43159141;C:25293742;G:34189453;T:34195924;N:141980 | 75 | 0 | 43159141 | 25293742 | 34189453 | 34195924 | 141980 | SRX5574316 | SRS4536874 | SRA865872 | University of California, Riverside|Environmental Sciences | University of California, Riverside | 1 | 0.77138 | 0.23812 | 0.80028 | 0.4318 | 73 | B | usable mapping rate | illumina | miseq | 3prime | poly_a | lexogen | bulk | unknown | unknown | United States | 2019-03-25 | Pharyngula | Embryo | Embryo Imprecise | All anatomical structures | |||||||||||||||||||||||||||
| 50906 | 50906 | SRR8784500 | SRX5574315 | SRS4536873 | SRP189394 | PRJNA511154 | Diphenyl Phosphate Induced Toxicity During Embryonic Development | PRJNA511154 | Other | The objective of this project was to rely on mRNA sequencing to determine the potential impacts of diphenyl phosphate DPHP on the embryonic transcriptome in zebrafish. | Control F 30 2 S5 | strain:5D|isolate:15|dev stage:30 hpf|sex:not applicable|tissue:embryo|BioSampleModel:Model organism or animal | Control F 30 2 S5 | Control F 30 2 S5 | Control F 30 2 S5 | QuantSeq three prime mRNA Seq Library Prep Kit FWD | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina MiniSeq | SRP189394 | F-30-2_S5_L001_R1_001.fastq.gz | fastq | 147514404.0 | 1959720.0 | F 30 2 S5 L001 R1 001.fastq.gz | 0:75.27 1:0 | A:45938492;C:26838492;G:37757031;T:36850878;N:129511 | 75 | 0 | 45938492 | 26838492 | 37757031 | 36850878 | 129511 | SRX5574315 | SRS4536873 | SRA865872 | University of California, Riverside|Environmental Sciences | University of California, Riverside | 1 | 0.7722 | 0.24196 | 0.79699 | 0.4319 | 76 | B | usable mapping rate | illumina | miseq | 3prime | poly_a | lexogen | bulk | unknown | unknown | United States | 2019-03-25 | Pharyngula | Embryo | Embryo Imprecise | All anatomical structures | |||||||||||||||||||||||||||
| 51775 | 51775 | SRR9850655 | SRX6605278 | SRS5170107 | SRP216607 | PRJNA529921 | mRNA Sequencing Identifies Liver as a Potential Target Organ for Triphenyl Phosphate in Embryonic Zebrafish | PRJNA529921 | Other | The objectives of this study were to 1 rely on mRNA sequencing to identify pathways before and post cardiac looping 30 hpf and 48 hpf respectively that may be impacted following exposure to 10 µM TPHP from 24 hpf 48 hpf and 2 determine whether pre treatment with 2 µM fenretinide from 24 hpf 30 hpf mitigates cardiotoxicity related pathways within embryos exposed to TPHP from xxx 48 hpf. | VC 30H 1 S12 rep2 | strain:5D|isolate:13|dev stage:30 hpf|sex:not applicable|tissue:embryo|BioSampleModel:Model organism or animal | VC 30H 1 S12 rep2 | VC 30H 1 S12 rep2 | VC 30H 1 S12 rep2 | QuantSeq three prime mRNA Seq Library Prep Kit FWD | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina MiniSeq | SRP216607 | VC-30H-1_S12_L001_R1_001-rep2.fastq.gz | fastq | 214451690.0 | 2857310.0 | VC 30H 1 S12 L001 R1 001 rep2.fastq.gz | 0:75.05 1:0 | A:70373156;C:40335646;G:56583371;T:46774752;N:384765 | 75 | 0 | 70373156 | 40335646 | 56583371 | 46774752 | 384765 | SRX6605278 | SRS5170107 | SRA928015 | University of California, Riverside|Environmental Sciences | University of California, Riverside | 1 | 0.79135 | 0.18986 | 0.81109 | 0.5745 | 76 | B | usable mapping rate | illumina | miseq | 3prime | poly_a | lexogen | bulk | unknown | unknown | United States | 2019-07-28 | Pharyngula | Embryo | Embryo Imprecise | All anatomical structures | |||||||||||||||||||||||||||
| 51776 | 51776 | SRR9850656 | SRX6605277 | SRS5170106 | SRP216607 | PRJNA529921 | mRNA Sequencing Identifies Liver as a Potential Target Organ for Triphenyl Phosphate in Embryonic Zebrafish | PRJNA529921 | Other | The objectives of this study were to 1 rely on mRNA sequencing to identify pathways before and post cardiac looping 30 hpf and 48 hpf respectively that may be impacted following exposure to 10 µM TPHP from 24 hpf 48 hpf and 2 determine whether pre treatment with 2 µM fenretinide from 24 hpf 30 hpf mitigates cardiotoxicity related pathways within embryos exposed to TPHP from xxx 48 hpf. | VC 30H 2 S5 rep2 | strain:5D|isolate:14|dev stage:30 hpf|sex:not applicable|tissue:embryo|BioSampleModel:Model organism or animal | VC 30H 2 S5 rep2 | VC 30H 2 S5 rep2 | VC 30H 2 S5 rep2 | QuantSeq three prime mRNA Seq Library Prep Kit FWD | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina MiniSeq | SRP216607 | VC-30H-2_S5_L001_R1_001-rep2.fastq.gz | fastq | 225831816.0 | 3013697.0 | VC 30H 2 S5 L001 R1 001 rep2.fastq.gz | 0:74.94 1:0 | A:74884869;C:41493176;G:59418803;T:49476791;N:558177 | 74 | 0 | 74884869 | 41493176 | 59418803 | 49476791 | 558177 | SRX6605277 | SRS5170106 | SRA928015 | University of California, Riverside|Environmental Sciences | University of California, Riverside | 1 | 0.79148 | 0.17245 | 0.81826 | 0.57312 | 75 | B | usable mapping rate | illumina | miseq | 3prime | poly_a | lexogen | bulk | unknown | unknown | United States | 2019-07-28 | Pharyngula | Embryo | Embryo Imprecise | All anatomical structures | |||||||||||||||||||||||||||
| 51779 | 51779 | SRR9850659 | SRX6605274 | SRS5170103 | SRP216607 | PRJNA529921 | mRNA Sequencing Identifies Liver as a Potential Target Organ for Triphenyl Phosphate in Embryonic Zebrafish | PRJNA529921 | Other | The objectives of this study were to 1 rely on mRNA sequencing to identify pathways before and post cardiac looping 30 hpf and 48 hpf respectively that may be impacted following exposure to 10 µM TPHP from 24 hpf 48 hpf and 2 determine whether pre treatment with 2 µM fenretinide from 24 hpf 30 hpf mitigates cardiotoxicity related pathways within embryos exposed to TPHP from xxx 48 hpf. | TPHP 30H 2 S4 rep2 | strain:5D|isolate:17|dev stage:30 hpf|sex:not applicable|tissue:embryo|BioSampleModel:Model organism or animal | TPHP 30H 2 S4 rep2 | TPHP 30H 2 S4 rep2 | TPHP 30H 2 S4 rep2 | QuantSeq three prime mRNA Seq Library Prep Kit FWD | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina MiniSeq | SRP216607 | TPHP-30H-2_S4_L001_R1_001-rep2.fastq.gz | fastq | 190443475.0 | 2534194.0 | TPHP 30H 2 S4 L001 R1 001 rep2.fastq.gz | 0:75.15 1:0 | A:61762406;C:35761803;G:46746491;T:45955520;N:217255 | 75 | 0 | 61762406 | 35761803 | 46746491 | 45955520 | 217255 | SRX6605274 | SRS5170103 | SRA928015 | University of California, Riverside|Environmental Sciences | University of California, Riverside | 1 | 0.82189 | 0.17354 | 0.80286 | 0.58945 | 76 | B | usable mapping rate | illumina | miseq | 3prime | poly_a | lexogen | bulk | unknown | unknown | United States | 2019-07-28 | Pharyngula | Embryo | Embryo Imprecise | All anatomical structures | |||||||||||||||||||||||||||
| 51780 | 51780 | SRR9850660 | SRX6605273 | SRS5170102 | SRP216607 | PRJNA529921 | mRNA Sequencing Identifies Liver as a Potential Target Organ for Triphenyl Phosphate in Embryonic Zebrafish | PRJNA529921 | Other | The objectives of this study were to 1 rely on mRNA sequencing to identify pathways before and post cardiac looping 30 hpf and 48 hpf respectively that may be impacted following exposure to 10 µM TPHP from 24 hpf 48 hpf and 2 determine whether pre treatment with 2 µM fenretinide from 24 hpf 30 hpf mitigates cardiotoxicity related pathways within embryos exposed to TPHP from xxx 48 hpf. | TPHP 30H 3 S3 rep2 | strain:5D|isolate:18|dev stage:30 hpf|sex:not applicable|tissue:embryo|BioSampleModel:Model organism or animal | TPHP 30H 3 S3 rep2 | TPHP 30H 3 S3 rep2 | TPHP 30H 3 S3 rep2 | QuantSeq three prime mRNA Seq Library Prep Kit FWD | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina MiniSeq | SRP216607 | TPHP-30H-3_S3_L001_R1_001-rep2.fastq.gz | fastq | 203756306.0 | 2710328.0 | TPHP 30H 3 S3 L001 R1 001 rep2.fastq.gz | 0:75.18 1:0 | A:66084783;C:37796994;G:51553200;T:48175069;N:146260 | 75 | 0 | 66084783 | 37796994 | 51553200 | 48175069 | 146260 | SRX6605273 | SRS5170102 | SRA928015 | University of California, Riverside|Environmental Sciences | University of California, Riverside | 1 | 0.81366 | 0.16393 | 0.80172 | 0.56399 | 75 | B | usable mapping rate | illumina | miseq | 3prime | poly_a | lexogen | bulk | unknown | unknown | United States | 2019-07-28 | Pharyngula | Embryo | Embryo Imprecise | All anatomical structures | |||||||||||||||||||||||||||
| 51781 | 51781 | SRR9850661 | SRX6605272 | SRS5170101 | SRP216607 | PRJNA529921 | mRNA Sequencing Identifies Liver as a Potential Target Organ for Triphenyl Phosphate in Embryonic Zebrafish | PRJNA529921 | Other | The objectives of this study were to 1 rely on mRNA sequencing to identify pathways before and post cardiac looping 30 hpf and 48 hpf respectively that may be impacted following exposure to 10 µM TPHP from 24 hpf 48 hpf and 2 determine whether pre treatment with 2 µM fenretinide from 24 hpf 30 hpf mitigates cardiotoxicity related pathways within embryos exposed to TPHP from xxx 48 hpf. | VC 30H 3 S11 rep2 | strain:5D|isolate:15|dev stage:30 hpf|sex:not applicable|tissue:embryo|BioSampleModel:Model organism or animal | VC 30H 3 S11 rep2 | VC 30H 3 S11 rep2 | VC 30H 3 S11 rep2 | QuantSeq three prime mRNA Seq Library Prep Kit FWD | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina MiniSeq | SRP216607 | VC-30H-3_S11_L001_R1_001-rep2.fastq.gz | fastq | 246195570.0 | 3284041.0 | VC 30H 3 S11 L001 R1 001 rep2.fastq.gz | 0:74.97 1:0 | A:81577760;C:45730136;G:69244229;T:49099097;N:544348 | 74 | 0 | 81577760 | 45730136 | 69244229 | 49099097 | 544348 | SRX6605272 | SRS5170101 | SRA928015 | University of California, Riverside|Environmental Sciences | University of California, Riverside | 1 | 0.76927 | 0.23225 | 0.83167 | 0.60203 | 75 | B | usable mapping rate | illumina | miseq | 3prime | poly_a | lexogen | bulk | unknown | unknown | United States | 2019-07-28 | Pharyngula | Embryo | Embryo Imprecise | All anatomical structures | |||||||||||||||||||||||||||
| 51782 | 51782 | SRR9850662 | SRX6605271 | SRS5170100 | SRP216607 | PRJNA529921 | mRNA Sequencing Identifies Liver as a Potential Target Organ for Triphenyl Phosphate in Embryonic Zebrafish | PRJNA529921 | Other | The objectives of this study were to 1 rely on mRNA sequencing to identify pathways before and post cardiac looping 30 hpf and 48 hpf respectively that may be impacted following exposure to 10 µM TPHP from 24 hpf 48 hpf and 2 determine whether pre treatment with 2 µM fenretinide from 24 hpf 30 hpf mitigates cardiotoxicity related pathways within embryos exposed to TPHP from xxx 48 hpf. | TPHP 30H 1 S10 rep2 | strain:5D|isolate:16|dev stage:30 hpf|sex:not applicable|tissue:embryo|BioSampleModel:Model organism or animal | TPHP 30H 1 S10 rep2 | TPHP 30H 1 S10 rep2 | TPHP 30H 1 S10 rep2 | QuantSeq three prime mRNA Seq Library Prep Kit FWD | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina MiniSeq | SRP216607 | TPHP-30H-1_S10_L001_R1_001-rep2.fastq.gz | fastq | 108576130.0 | 1445012.0 | TPHP 30H 1 S10 L001 R1 001 rep2.fastq.gz | 0:75.14 1:0 | A:34532346;C:20830575;G:28514938;T:24567029;N:131242 | 75 | 0 | 34532346 | 20830575 | 28514938 | 24567029 | 131242 | SRX6605271 | SRS5170100 | SRA928015 | University of California, Riverside|Environmental Sciences | University of California, Riverside | 1 | 0.81422 | 0.19725 | 0.81602 | 0.59356 | 75 | B | usable mapping rate | illumina | miseq | 3prime | poly_a | lexogen | bulk | unknown | unknown | United States | 2019-07-28 | Pharyngula | Embryo | Embryo Imprecise | All anatomical structures | |||||||||||||||||||||||||||
| 51787 | 51787 | SRR9850667 | SRX6605266 | SRS5170095 | SRP216607 | PRJNA529921 | mRNA Sequencing Identifies Liver as a Potential Target Organ for Triphenyl Phosphate in Embryonic Zebrafish | PRJNA529921 | Other | The objectives of this study were to 1 rely on mRNA sequencing to identify pathways before and post cardiac looping 30 hpf and 48 hpf respectively that may be impacted following exposure to 10 µM TPHP from 24 hpf 48 hpf and 2 determine whether pre treatment with 2 µM fenretinide from 24 hpf 30 hpf mitigates cardiotoxicity related pathways within embryos exposed to TPHP from xxx 48 hpf. | VC 30H 3 S9 rep1 | strain:5D|isolate:3|dev stage:30 hpf|sex:not applicable|tissue:embryo|BioSampleModel:Model organism or animal | VC 30H 3 S9 rep1 | VC 30H 3 S9 rep1 | VC 30H 3 S9 rep1 | QuantSeq three prime mRNA Seq Library Prep Kit FWD | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina MiniSeq | SRP216607 | VC-30H-3_S9_L001_R1_001-rep1.fastq.gz | fastq | 179751806.0 | 2398199.0 | VC 30H 3 S9 L001 R1 001 rep1.fastq.gz | 0:74.95 1:0 | A:59567786;C:33434792;G:50404279;T:35931343;N:413606 | 74 | 0 | 59567786 | 33434792 | 50404279 | 35931343 | 413606 | SRX6605266 | SRS5170095 | SRA928015 | University of California, Riverside|Environmental Sciences | University of California, Riverside | 1 | 0.7688 | 0.23182 | 0.83272 | 0.5965 | 76 | B | usable mapping rate | illumina | miseq | 3prime | poly_a | lexogen | bulk | unknown | unknown | United States | 2019-07-28 | Pharyngula | Embryo | Embryo Imprecise | All anatomical structures | |||||||||||||||||||||||||||
| 51788 | 51788 | SRR9850668 | SRX6605265 | SRS5170094 | SRP216607 | PRJNA529921 | mRNA Sequencing Identifies Liver as a Potential Target Organ for Triphenyl Phosphate in Embryonic Zebrafish | PRJNA529921 | Other | The objectives of this study were to 1 rely on mRNA sequencing to identify pathways before and post cardiac looping 30 hpf and 48 hpf respectively that may be impacted following exposure to 10 µM TPHP from 24 hpf 48 hpf and 2 determine whether pre treatment with 2 µM fenretinide from 24 hpf 30 hpf mitigates cardiotoxicity related pathways within embryos exposed to TPHP from xxx 48 hpf. | TPHP 30H 1 S7 rep1 | strain:5D|isolate:4|dev stage:30 hpf|sex:not applicable|tissue:embryo|BioSampleModel:Model organism or animal | TPHP 30H 1 S7 rep1 | TPHP 30H 1 S7 rep1 | TPHP 30H 1 S7 rep1 | QuantSeq three prime mRNA Seq Library Prep Kit FWD | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina MiniSeq | SRP216607 | TPHP-30H-1_S7_L001_R1_001-rep1.fastq.gz | fastq | 152844797.0 | 2032948.0 | TPHP 30H 1 S7 L001 R1 001 rep1.fastq.gz | 0:75.18 1:0 | A:48638640;C:29022484;G:38893945;T:36117325;N:172403 | 75 | 0 | 48638640 | 29022484 | 38893945 | 36117325 | 172403 | SRX6605265 | SRS5170094 | SRA928015 | University of California, Riverside|Environmental Sciences | University of California, Riverside | 1 | 0.8224 | 0.18948 | 0.80643 | 0.60363 | 75 | B | usable mapping rate | illumina | miseq | 3prime | poly_a | lexogen | bulk | unknown | unknown | United States | 2019-07-28 | Pharyngula | Embryo | Embryo Imprecise | All anatomical structures | |||||||||||||||||||||||||||
| 51789 | 51789 | SRR9850669 | SRX6605264 | SRS5170093 | SRP216607 | PRJNA529921 | mRNA Sequencing Identifies Liver as a Potential Target Organ for Triphenyl Phosphate in Embryonic Zebrafish | PRJNA529921 | Other | The objectives of this study were to 1 rely on mRNA sequencing to identify pathways before and post cardiac looping 30 hpf and 48 hpf respectively that may be impacted following exposure to 10 µM TPHP from 24 hpf 48 hpf and 2 determine whether pre treatment with 2 µM fenretinide from 24 hpf 30 hpf mitigates cardiotoxicity related pathways within embryos exposed to TPHP from xxx 48 hpf. | VC 30H 1 S12 rep1 | strain:5D|isolate:1|dev stage:30 hpf|sex:not applicable|tissue:embryo|BioSampleModel:Model organism or animal | VC 30H 1 S12 rep1 | VC 30H 1 S12 rep1 | VC 30H 1 S12 rep1 | QuantSeq three prime mRNA Seq Library Prep Kit FWD | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina MiniSeq | SRP216607 | VC-30H-1_S12_L001_R1_001-rep1.fastq.gz | fastq | 188024539.0 | 2505350.0 | VC 30H 1 S12 L001 R1 001 rep1.fastq.gz | 0:75.05 1:0 | A:61707151;C:35389050;G:49592916;T:40988051;N:347371 | 75 | 0 | 61707151 | 35389050 | 49592916 | 40988051 | 347371 | SRX6605264 | SRS5170093 | SRA928015 | University of California, Riverside|Environmental Sciences | University of California, Riverside | 1 | 0.78842 | 0.18566 | 0.81221 | 0.58599 | 76 | B | usable mapping rate | illumina | miseq | 3prime | poly_a | lexogen | bulk | unknown | unknown | United States | 2019-07-28 | Pharyngula | Embryo | Embryo Imprecise | All anatomical structures | |||||||||||||||||||||||||||
| 51790 | 51790 | SRR9850670 | SRX6605263 | SRS5170092 | SRP216607 | PRJNA529921 | mRNA Sequencing Identifies Liver as a Potential Target Organ for Triphenyl Phosphate in Embryonic Zebrafish | PRJNA529921 | Other | The objectives of this study were to 1 rely on mRNA sequencing to identify pathways before and post cardiac looping 30 hpf and 48 hpf respectively that may be impacted following exposure to 10 µM TPHP from 24 hpf 48 hpf and 2 determine whether pre treatment with 2 µM fenretinide from 24 hpf 30 hpf mitigates cardiotoxicity related pathways within embryos exposed to TPHP from xxx 48 hpf. | VC 30H 2 S10 rep1 | strain:5D|isolate:2|dev stage:30 hpf|sex:not applicable|tissue:embryo|BioSampleModel:Model organism or animal | VC 30H 2 S10 rep1 | VC 30H 2 S10 rep1 | VC 30H 2 S10 rep1 | QuantSeq three prime mRNA Seq Library Prep Kit FWD | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina MiniSeq | SRP216607 | VC-30H-2_S10_L001_R1_001-rep1.fastq.gz | fastq | 208974583.0 | 2789057.0 | VC 30H 2 S10 L001 R1 001 rep1.fastq.gz | 0:74.93 1:0 | A:69392953;C:38416462;G:54829339;T:45809878;N:525951 | 74 | 0 | 69392953 | 38416462 | 54829339 | 45809878 | 525951 | SRX6605263 | SRS5170092 | SRA928015 | University of California, Riverside|Environmental Sciences | University of California, Riverside | 1 | 0.78872 | 0.17214 | 0.81661 | 0.56755 | 76 | B | usable mapping rate | illumina | miseq | 3prime | poly_a | lexogen | bulk | unknown | unknown | United States | 2019-07-28 | Pharyngula | Embryo | Embryo Imprecise | All anatomical structures | |||||||||||||||||||||||||||
| 51793 | 51793 | SRR9850673 | SRX6605260 | SRS5170089 | SRP216607 | PRJNA529921 | mRNA Sequencing Identifies Liver as a Potential Target Organ for Triphenyl Phosphate in Embryonic Zebrafish | PRJNA529921 | Other | The objectives of this study were to 1 rely on mRNA sequencing to identify pathways before and post cardiac looping 30 hpf and 48 hpf respectively that may be impacted following exposure to 10 µM TPHP from 24 hpf 48 hpf and 2 determine whether pre treatment with 2 µM fenretinide from 24 hpf 30 hpf mitigates cardiotoxicity related pathways within embryos exposed to TPHP from xxx 48 hpf. | TPHP 30H 2 S6 rep1 | strain:5D|isolate:5|dev stage:30 hpf|sex:not applicable|tissue:embryo|BioSampleModel:Model organism or animal | TPHP 30H 2 S6 rep1 | TPHP 30H 2 S6 rep1 | TPHP 30H 2 S6 rep1 | QuantSeq three prime mRNA Seq Library Prep Kit FWD | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina MiniSeq | SRP216607 | TPHP-30H-2_S6_L001_R1_001-rep1.fastq.gz | fastq | 194841362.0 | 2592817.0 | TPHP 30H 2 S6 L001 R1 001 rep1.fastq.gz | 0:75.15 1:0 | A:63263665;C:36573739;G:47683114;T:47088583;N:232261 | 75 | 0 | 63263665 | 36573739 | 47683114 | 47088583 | 232261 | SRX6605260 | SRS5170089 | SRA928015 | University of California, Riverside|Environmental Sciences | University of California, Riverside | 1 | 0.82011 | 0.1723 | 0.80338 | 0.58394 | 76 | B | usable mapping rate | illumina | miseq | 3prime | poly_a | lexogen | bulk | unknown | unknown | United States | 2019-07-28 | Pharyngula | Embryo | Embryo Imprecise | All anatomical structures | |||||||||||||||||||||||||||
| 51794 | 51794 | SRR9850674 | SRX6605259 | SRS5170088 | SRP216607 | PRJNA529921 | mRNA Sequencing Identifies Liver as a Potential Target Organ for Triphenyl Phosphate in Embryonic Zebrafish | PRJNA529921 | Other | The objectives of this study were to 1 rely on mRNA sequencing to identify pathways before and post cardiac looping 30 hpf and 48 hpf respectively that may be impacted following exposure to 10 µM TPHP from 24 hpf 48 hpf and 2 determine whether pre treatment with 2 µM fenretinide from 24 hpf 30 hpf mitigates cardiotoxicity related pathways within embryos exposed to TPHP from xxx 48 hpf. | TPHP 30H 3 S4 rep1 | strain:5D|isolate:6|dev stage:30 hpf|sex:not applicable|tissue:embryo|BioSampleModel:Model organism or animal | TPHP 30H 3 S4 rep1 | TPHP 30H 3 S4 rep1 | TPHP 30H 3 S4 rep1 | QuantSeq three prime mRNA Seq Library Prep Kit FWD | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina MiniSeq | SRP216607 | TPHP-30H-3_S4_L001_R1_001-rep1.fastq.gz | fastq | 168122597.0 | 2236679.0 | TPHP 30H 3 S4 L001 R1 001 rep1.fastq.gz | 0:75.17 1:0 | A:54706169;C:31138406;G:42526510;T:39600704;N:150808 | 75 | 0 | 54706169 | 31138406 | 42526510 | 39600704 | 150808 | SRX6605259 | SRS5170088 | SRA928015 | University of California, Riverside|Environmental Sciences | University of California, Riverside | 1 | 0.81103 | 0.16383 | 0.80223 | 0.568 | 75 | B | usable mapping rate | illumina | miseq | 3prime | poly_a | lexogen | bulk | unknown | unknown | United States | 2019-07-28 | Pharyngula | Embryo | Embryo Imprecise | All anatomical structures | |||||||||||||||||||||||||||
| 52310 | 52310 | SRR10490653 | SRX7179784 | SRS5686601 | SRP230531 | PRJNA544341 | PPAR? agonist induced disruption of dorsoventral patterning in zebrafish | PRJNA544341 | Other | The objectives of this study were to rely on mRNA sequencing to identify pathways that may be impacted following exposure to ciglitazone in the presence or absence of dorsomorphin within the first 24 h of development. | DMP 24hpf 2 S1 | strain:5D|isolate:10|dev stage:24 hpf|sex:not applicable|tissue:embryo|BioSampleModel:Model organism or animal | DMP 24hpf 2 S1 | DMP 24hpf 2 S1 | DMP 24hpf 2 S1 | QuantSeq three prime mRNA Seq Library Prep Kit FWD | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina MiniSeq | SRP230531 | DMP-24hpf-2_S1_L001_R1_001.fastq.gz | fastq | 154522234.0 | 2051336.0 | DMP 24hpf 2 S1 L001 R1 001.fastq.gz | 0:75.33 1:0 | A:53760130;C:29133871;G:35537868;T:35993942;N:96423 | 75 | 0 | 53760130 | 29133871 | 35537868 | 35993942 | 96423 | SRX7179784 | SRS5686601 | SRA998591 | University of California, Riverside|Environmental Sciences | University of California, Riverside | 1 | 0.7423 | 0.19917 | 0.81264 | 0.62122 | 76 | B | usable mapping rate | illumina | miseq | 3prime | poly_a | lexogen | bulk | unknown | unknown | United States | 2019-11-19 | Pharyngula | Embryo | Embryo Imprecise | All anatomical structures | |||||||||||||||||||||||||||
| 52311 | 52311 | SRR10490654 | SRX7179783 | SRS5686600 | SRP230531 | PRJNA544341 | PPAR? agonist induced disruption of dorsoventral patterning in zebrafish | PRJNA544341 | Other | The objectives of this study were to rely on mRNA sequencing to identify pathways that may be impacted following exposure to ciglitazone in the presence or absence of dorsomorphin within the first 24 h of development. | DMP 24hpf 1 S11 | strain:5D|isolate:9|dev stage:24 hpf|sex:not applicable|tissue:embryo|BioSampleModel:Model organism or animal | DMP 24hpf 1 S11 | DMP 24hpf 1 S11 | DMP 24hpf 1 S11 | QuantSeq three prime mRNA Seq Library Prep Kit FWD | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina MiniSeq | SRP230531 | DMP-24hpf-1_S11_L001_R1_001.fastq.gz | fastq | 164424909.0 | 2182222.0 | DMP 24hpf 1 S11 L001 R1 001.fastq.gz | 0:75.35 1:0 | A:57308208;C:30191855;G:37128149;T:39702440;N:94257 | 75 | 0 | 57308208 | 30191855 | 37128149 | 39702440 | 94257 | SRX7179783 | SRS5686600 | SRA998591 | University of California, Riverside|Environmental Sciences | University of California, Riverside | 1 | 0.75187 | 0.17722 | 0.80424 | 0.59348 | 75 | B | usable mapping rate | illumina | miseq | 3prime | poly_a | lexogen | bulk | unknown | unknown | United States | 2019-11-19 | Pharyngula | Embryo | Embryo Imprecise | All anatomical structures | |||||||||||||||||||||||||||
| 52312 | 52312 | SRR10490655 | SRX7179782 | SRS5686599 | SRP230531 | PRJNA544341 | PPAR? agonist induced disruption of dorsoventral patterning in zebrafish | PRJNA544341 | Other | The objectives of this study were to rely on mRNA sequencing to identify pathways that may be impacted following exposure to ciglitazone in the presence or absence of dorsomorphin within the first 24 h of development. | CigDMP 24hpf 4 S3 | strain:5D|isolate:8|dev stage:24 hpf|sex:not applicable|tissue:embryo|BioSampleModel:Model organism or animal | CigDMP 24hpf 4 S3 | CigDMP 24hpf 4 S3 | CigDMP 24hpf 4 S3 | QuantSeq three prime mRNA Seq Library Prep Kit FWD | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina MiniSeq | SRP230531 | CigDMP-24hpf-4_S3_L001_R1_001.fastq.gz | fastq | 168508233.0 | 2237284.0 | CigDMP 24hpf 4 S3 L001 R1 001.fastq.gz | 0:75.32 1:0 | A:61448456;C:29365573;G:37720412;T:39833702;N:140090 | 75 | 0 | 61448456 | 29365573 | 37720412 | 39833702 | 140090 | SRX7179782 | SRS5686599 | SRA998591 | University of California, Riverside|Environmental Sciences | University of California, Riverside | 1 | 0.72054 | 0.12417 | 0.82718 | 0.6432 | 76 | B | usable mapping rate | illumina | miseq | 3prime | poly_a | lexogen | bulk | unknown | unknown | United States | 2019-11-19 | Pharyngula | Embryo | Embryo Imprecise | All anatomical structures | |||||||||||||||||||||||||||
| 52313 | 52313 | SRR10490656 | SRX7179781 | SRS5686598 | SRP230531 | PRJNA544341 | PPAR? agonist induced disruption of dorsoventral patterning in zebrafish | PRJNA544341 | Other | The objectives of this study were to rely on mRNA sequencing to identify pathways that may be impacted following exposure to ciglitazone in the presence or absence of dorsomorphin within the first 24 h of development. | CigDMP 24hpf 3 S12 | strain:5D|isolate:7|dev stage:24 hpf|sex:not applicable|tissue:embryo|BioSampleModel:Model organism or animal | CigDMP 24hpf 3 S12 | CigDMP 24hpf 3 S12 | CigDMP 24hpf 3 S12 | QuantSeq three prime mRNA Seq Library Prep Kit FWD | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina MiniSeq | SRP230531 | CigDMP-24hpf-3_S12_L001_R1_001.fastq.gz | fastq | 145084817.0 | 1924713.0 | CigDMP 24hpf 3 S12 L001 R1 001.fastq.gz | 0:75.38 1:0 | A:46287612;C:27449291;G:32774875;T:38473634;N:99405 | 75 | 0 | 46287612 | 27449291 | 32774875 | 38473634 | 99405 | SRX7179781 | SRS5686598 | SRA998591 | University of California, Riverside|Environmental Sciences | University of California, Riverside | 1 | 0.83194 | 0.14768 | 0.8116 | 0.60811 | 76 | B | usable mapping rate | illumina | miseq | 3prime | poly_a | lexogen | bulk | unknown | unknown | United States | 2019-11-19 | Pharyngula | Embryo | Embryo Imprecise | All anatomical structures | |||||||||||||||||||||||||||
| 52314 | 52314 | SRR10490657 | SRX7179780 | SRS5686597 | SRP230531 | PRJNA544341 | PPAR? agonist induced disruption of dorsoventral patterning in zebrafish | PRJNA544341 | Other | The objectives of this study were to rely on mRNA sequencing to identify pathways that may be impacted following exposure to ciglitazone in the presence or absence of dorsomorphin within the first 24 h of development. | CigDMP 24hpf 2 S13 | strain:5D|isolate:6|dev stage:24 hpf|sex:not applicable|tissue:embryo|BioSampleModel:Model organism or animal | CigDMP 24hpf 2 S13 | CigDMP 24hpf 2 S13 | CigDMP 24hpf 2 S13 | QuantSeq three prime mRNA Seq Library Prep Kit FWD | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina MiniSeq | SRP230531 | CigDMP-24hpf-2_S13_L001_R1_001.fastq.gz | fastq | 282221813.0 | 3749290.0 | CigDMP 24hpf 2 S13 L001 R1 001.fastq.gz | 0:75.27 1:0 | A:96636505;C:53828518;G:69820577;T:61607663;N:328550 | 75 | 0 | 96636505 | 53828518 | 69820577 | 61607663 | 328550 | SRX7179780 | SRS5686597 | SRA998591 | University of California, Riverside|Environmental Sciences | University of California, Riverside | 1 | 0.70551 | 0.17608 | 0.83842 | 0.52731 | 76 | B | usable mapping rate | illumina | miseq | 3prime | poly_a | lexogen | bulk | unknown | unknown | United States | 2019-11-19 | Pharyngula | Embryo | Embryo Imprecise | All anatomical structures | |||||||||||||||||||||||||||
| 52315 | 52315 | SRR10490658 | SRX7179779 | SRS5686596 | SRP230531 | PRJNA544341 | PPAR? agonist induced disruption of dorsoventral patterning in zebrafish | PRJNA544341 | Other | The objectives of this study were to rely on mRNA sequencing to identify pathways that may be impacted following exposure to ciglitazone in the presence or absence of dorsomorphin within the first 24 h of development. | CigDMP 24hpf 1 S10 | strain:5D|isolate:5|dev stage:24 hpf|sex:not applicable|tissue:embryo|BioSampleModel:Model organism or animal | CigDMP 24hpf 1 S10 | CigDMP 24hpf 1 S10 | CigDMP 24hpf 1 S10 | QuantSeq three prime mRNA Seq Library Prep Kit FWD | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina MiniSeq | SRP230531 | CigDMP-24hpf-1_S10_L001_R1_001.fastq.gz | fastq | 166244445.0 | 2206862.0 | CigDMP 24hpf 1 S10 L001 R1 001.fastq.gz | 0:75.33 1:0 | A:56629344;C:30735682;G:37080320;T:41653531;N:145568 | 75 | 0 | 56629344 | 30735682 | 37080320 | 41653531 | 145568 | SRX7179779 | SRS5686596 | SRA998591 | University of California, Riverside|Environmental Sciences | University of California, Riverside | 1 | 0.79315 | 0.14174 | 0.81199 | 0.59223 | 75 | B | usable mapping rate | illumina | miseq | 3prime | poly_a | lexogen | bulk | unknown | unknown | United States | 2019-11-19 | Pharyngula | Embryo | Embryo Imprecise | All anatomical structures | |||||||||||||||||||||||||||
| 52316 | 52316 | SRR10490659 | SRX7179778 | SRS5686595 | SRP230531 | PRJNA544341 | PPAR? agonist induced disruption of dorsoventral patterning in zebrafish | PRJNA544341 | Other | The objectives of this study were to rely on mRNA sequencing to identify pathways that may be impacted following exposure to ciglitazone in the presence or absence of dorsomorphin within the first 24 h of development. | Cig 24hpf 4 S2 | strain:5D|isolate:4|dev stage:24 hpf|sex:not applicable|tissue:embryo|BioSampleModel:Model organism or animal | Cig 24hpf 4 S2 | Cig 24hpf 4 S2 | Cig 24hpf 4 S2 | QuantSeq three prime mRNA Seq Library Prep Kit FWD | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina MiniSeq | SRP230531 | Cig-24hpf-4_S2_L001_R1_001.fastq.gz | fastq | 365700453.0 | 4854033.0 | Cig 24hpf 4 S2 L001 R1 001.fastq.gz | 0:75.34 1:0 | A:127185124;C:68098631;G:85365420;T:84769467;N:281811 | 75 | 0 | 127185124 | 68098631 | 85365420 | 84769467 | 281811 | SRX7179778 | SRS5686595 | SRA998591 | University of California, Riverside|Environmental Sciences | University of California, Riverside | 1 | 0.75787 | 0.167 | 0.82024 | 0.65882 | 75 | B | usable mapping rate | illumina | miseq | 3prime | poly_a | lexogen | bulk | unknown | unknown | United States | 2019-11-19 | Pharyngula | Embryo | Embryo Imprecise | All anatomical structures | |||||||||||||||||||||||||||
| 52317 | 52317 | SRR10490660 | SRX7179777 | SRS5686594 | SRP230531 | PRJNA544341 | PPAR? agonist induced disruption of dorsoventral patterning in zebrafish | PRJNA544341 | Other | The objectives of this study were to rely on mRNA sequencing to identify pathways that may be impacted following exposure to ciglitazone in the presence or absence of dorsomorphin within the first 24 h of development. | Cig 24hpf 3 S7 | strain:5D|isolate:3|dev stage:24 hpf|sex:not applicable|tissue:embryo|BioSampleModel:Model organism or animal | Cig 24hpf 3 S7 | Cig 24hpf 3 S7 | Cig 24hpf 3 S7 | QuantSeq three prime mRNA Seq Library Prep Kit FWD | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina MiniSeq | SRP230531 | Cig-24hpf-3_S7_L001_R1_001.fastq.gz | fastq | 161187457.0 | 2138808.0 | Cig 24hpf 3 S7 L001 R1 001.fastq.gz | 0:75.36 1:0 | A:56647234;C:30651392;G:36617181;T:37188808;N:82842 | 75 | 0 | 56647234 | 30651392 | 36617181 | 37188808 | 82842 | SRX7179777 | SRS5686594 | SRA998591 | University of California, Riverside|Environmental Sciences | University of California, Riverside | 1 | 0.76327 | 0.17071 | 0.81688 | 0.66245 | 76 | B | usable mapping rate | illumina | miseq | 3prime | poly_a | lexogen | bulk | unknown | unknown | United States | 2019-11-19 | Pharyngula | Embryo | Embryo Imprecise | All anatomical structures | |||||||||||||||||||||||||||
| 52318 | 52318 | SRR10490661 | SRX7179776 | SRS5686593 | SRP230531 | PRJNA544341 | PPAR? agonist induced disruption of dorsoventral patterning in zebrafish | PRJNA544341 | Other | The objectives of this study were to rely on mRNA sequencing to identify pathways that may be impacted following exposure to ciglitazone in the presence or absence of dorsomorphin within the first 24 h of development. | DMSO 24hpf 4 S9 | strain:5D|isolate:16|dev stage:24 hpf|sex:not applicable|tissue:embryo|BioSampleModel:Model organism or animal | DMSO 24hpf 4 S9 | DMSO 24hpf 4 S9 | DMSO 24hpf 4 S9 | QuantSeq three prime mRNA Seq Library Prep Kit FWD | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina MiniSeq | SRP230531 | DMSO-24hpf-4_S9_L001_R1_001.fastq.gz | fastq | 126406328.0 | 1678421.0 | DMSO 24hpf 4 S9 L001 R1 001.fastq.gz | 0:75.31 1:0 | A:45620840;C:24053460;G:28105201;T:28582915;N:43912 | 75 | 0 | 45620840 | 24053460 | 28105201 | 28582915 | 43912 | SRX7179776 | SRS5686593 | SRA998591 | University of California, Riverside|Environmental Sciences | University of California, Riverside | 1 | 0.7399 | 0.22979 | 0.82185 | 0.62477 | 75 | B | usable mapping rate | illumina | miseq | 3prime | poly_a | lexogen | bulk | unknown | unknown | United States | 2019-11-19 | Pharyngula | Embryo | Embryo Imprecise | All anatomical structures | |||||||||||||||||||||||||||
| 52319 | 52319 | SRR10490662 | SRX7179775 | SRS5686592 | SRP230531 | PRJNA544341 | PPAR? agonist induced disruption of dorsoventral patterning in zebrafish | PRJNA544341 | Other | The objectives of this study were to rely on mRNA sequencing to identify pathways that may be impacted following exposure to ciglitazone in the presence or absence of dorsomorphin within the first 24 h of development. | DMSO 24hpf 3 S15 | strain:5D|isolate:15|dev stage:24 hpf|sex:not applicable|tissue:embryo|BioSampleModel:Model organism or animal | DMSO 24hpf 3 S15 | DMSO 24hpf 3 S15 | DMSO 24hpf 3 S15 | QuantSeq three prime mRNA Seq Library Prep Kit FWD | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina MiniSeq | SRP230531 | DMSO-24hpf-3_S15_L001_R1_001.fastq.gz | fastq | 123251397.0 | 1635854.0 | DMSO 24hpf 3 S15 L001 R1 001.fastq.gz | 0:75.34 1:0 | A:43811855;C:23533043;G:27236165;T:28625055;N:45279 | 75 | 0 | 43811855 | 23533043 | 27236165 | 28625055 | 45279 | SRX7179775 | SRS5686592 | SRA998591 | University of California, Riverside|Environmental Sciences | University of California, Riverside | 1 | 0.75956 | 0.23348 | 0.81302 | 0.62477 | 76 | B | usable mapping rate | illumina | miseq | 3prime | poly_a | lexogen | bulk | unknown | unknown | United States | 2019-11-19 | Pharyngula | Embryo | Embryo Imprecise | All anatomical structures | |||||||||||||||||||||||||||
| 52320 | 52320 | SRR10490663 | SRX7179774 | SRS5686591 | SRP230531 | PRJNA544341 | PPAR? agonist induced disruption of dorsoventral patterning in zebrafish | PRJNA544341 | Other | The objectives of this study were to rely on mRNA sequencing to identify pathways that may be impacted following exposure to ciglitazone in the presence or absence of dorsomorphin within the first 24 h of development. | DMSO 24hpf 2 S16.1 | strain:5D|isolate:14|dev stage:24 hpf|sex:not applicable|tissue:embryo|BioSampleModel:Model organism or animal | DMSO 24hpf 2 S16.1 | DMSO 24hpf 2 S16.1 | DMSO 24hpf 2 S16.1 | QuantSeq three prime mRNA Seq Library Prep Kit FWD | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina MiniSeq | SRP230531 | DMSO-24hpf-2_S16_L001_R1_001.fastq.gz | fastq | 132048224.0 | 1754609.0 | DMSO 24hpf 2 S16 L001 R1 001.fastq.gz | 0:75.26 1:0 | A:48638287;C:24900943;G:30060914;T:28333971;N:114109 | 75 | 0 | 48638287 | 24900943 | 30060914 | 28333971 | 114109 | SRX7179774 | SRS5686591 | SRA998591 | University of California, Riverside|Environmental Sciences | University of California, Riverside | 1 | 0.70403 | 0.21158 | 0.82944 | 0.6256 | 76 | B | usable mapping rate | illumina | miseq | 3prime | poly_a | lexogen | bulk | unknown | unknown | United States | 2019-11-19 | Pharyngula | Embryo | Embryo Imprecise | All anatomical structures | |||||||||||||||||||||||||||
| 52321 | 52321 | SRR10490664 | SRX7179773 | SRS5686590 | SRP230531 | PRJNA544341 | PPAR? agonist induced disruption of dorsoventral patterning in zebrafish | PRJNA544341 | Other | The objectives of this study were to rely on mRNA sequencing to identify pathways that may be impacted following exposure to ciglitazone in the presence or absence of dorsomorphin within the first 24 h of development. | DMSO 24hpf 1 S14 | strain:5D|isolate:13|dev stage:24 hpf|sex:not applicable|tissue:embryo|BioSampleModel:Model organism or animal | DMSO 24hpf 1 S14 | DMSO 24hpf 1 S14 | DMSO 24hpf 1 S14 | QuantSeq three prime mRNA Seq Library Prep Kit FWD | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina MiniSeq | SRP230531 | DMSO-24hpf-1_S14_L001_R1_001.fastq.gz | fastq | 139193171.0 | 1848793.0 | DMSO 24hpf 1 S14 L001 R1 001.fastq.gz | 0:75.29 1:0 | A:52588588;C:25826387;G:32607820;T:28094765;N:75611 | 75 | 0 | 52588588 | 25826387 | 32607820 | 28094765 | 75611 | SRX7179773 | SRS5686590 | SRA998591 | University of California, Riverside|Environmental Sciences | University of California, Riverside | 1 | 0.66115 | 0.21183 | 0.83871 | 0.6129 | 76 | B | usable mapping rate | illumina | miseq | 3prime | poly_a | lexogen | bulk | unknown | unknown | United States | 2019-11-19 | Pharyngula | Embryo | Embryo Imprecise | All anatomical structures | |||||||||||||||||||||||||||
| 52322 | 52322 | SRR10490665 | SRX7179772 | SRS5686589 | SRP230531 | PRJNA544341 | PPAR? agonist induced disruption of dorsoventral patterning in zebrafish | PRJNA544341 | Other | The objectives of this study were to rely on mRNA sequencing to identify pathways that may be impacted following exposure to ciglitazone in the presence or absence of dorsomorphin within the first 24 h of development. | DMP 24hpf 4 S6 | strain:5D|isolate:12|dev stage:24 hpf|sex:not applicable|tissue:embryo|BioSampleModel:Model organism or animal | DMP 24hpf 4 S6 | DMP 24hpf 4 S6 | DMP 24hpf 4 S6 | QuantSeq three prime mRNA Seq Library Prep Kit FWD | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina MiniSeq | SRP230531 | DMP-24hpf-4_S6_L001_R1_001.fastq.gz | fastq | 226343412.0 | 3004979.0 | DMP 24hpf 4 S6 L001 R1 001.fastq.gz | 0:75.32 1:0 | A:78184324;C:41397790;G:50552220;T:56036286;N:172792 | 75 | 0 | 78184324 | 41397790 | 50552220 | 56036286 | 172792 | SRX7179772 | SRS5686589 | SRA998591 | University of California, Riverside|Environmental Sciences | University of California, Riverside | 1 | 0.76278 | 0.12992 | 0.81134 | 0.56376 | 73 | B | usable mapping rate | illumina | miseq | 3prime | poly_a | lexogen | bulk | unknown | unknown | United States | 2019-11-19 | Pharyngula | Embryo | Embryo Imprecise | All anatomical structures | |||||||||||||||||||||||||||
| 52323 | 52323 | SRR10490666 | SRX7179771 | SRS5686588 | SRP230531 | PRJNA544341 | PPAR? agonist induced disruption of dorsoventral patterning in zebrafish | PRJNA544341 | Other | The objectives of this study were to rely on mRNA sequencing to identify pathways that may be impacted following exposure to ciglitazone in the presence or absence of dorsomorphin within the first 24 h of development. | DMP 24hpf 3 S4 | strain:5D|isolate:11|dev stage:24 hpf|sex:not applicable|tissue:embryo|BioSampleModel:Model organism or animal | DMP 24hpf 3 S4 | DMP 24hpf 3 S4 | DMP 24hpf 3 S4 | QuantSeq three prime mRNA Seq Library Prep Kit FWD | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina MiniSeq | SRP230531 | DMP-24hpf-3_S4_L001_R1_001.fastq.gz | fastq | 175279417.0 | 2327885.0 | DMP 24hpf 3 S4 L001 R1 001.fastq.gz | 0:75.30 1:0 | A:60956678;C:31641832;G:39622230;T:42845005;N:213672 | 75 | 0 | 60956678 | 31641832 | 39622230 | 42845005 | 213672 | SRX7179771 | SRS5686588 | SRA998591 | University of California, Riverside|Environmental Sciences | University of California, Riverside | 1 | 0.75785 | 0.13198 | 0.81008 | 0.5685 | 72 | B | usable mapping rate | illumina | miseq | 3prime | poly_a | lexogen | bulk | unknown | unknown | United States | 2019-11-19 | Pharyngula | Embryo | Embryo Imprecise | All anatomical structures | |||||||||||||||||||||||||||
| 52324 | 52324 | SRR10490667 | SRX7179770 | SRS5686587 | SRP230531 | PRJNA544341 | PPAR? agonist induced disruption of dorsoventral patterning in zebrafish | PRJNA544341 | Other | The objectives of this study were to rely on mRNA sequencing to identify pathways that may be impacted following exposure to ciglitazone in the presence or absence of dorsomorphin within the first 24 h of development. | Cig 24hpf 2 S5 | strain:5D|isolate:2|dev stage:24 hpf|sex:not applicable|tissue:embryo|BioSampleModel:Model organism or animal | Cig 24hpf 2 S5 | Cig 24hpf 2 S5 | Cig 24hpf 2 S5 | QuantSeq three prime mRNA Seq Library Prep Kit FWD | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina MiniSeq | SRP230531 | Cig-24hpf-2_S5_L001_R1_001.fastq.gz | fastq | 135561364.0 | 1798064.0 | Cig 24hpf 2 S5 L001 R1 001.fastq.gz | 0:75.39 1:0 | A:48402460;C:24975846;G:31300454;T:30853581;N:29023 | 75 | 0 | 48402460 | 24975846 | 31300454 | 30853581 | 29023 | SRX7179770 | SRS5686587 | SRA998591 | University of California, Riverside|Environmental Sciences | University of California, Riverside | 1 | 0.72836 | 0.17494 | 0.82394 | 0.64987 | 75 | B | usable mapping rate | illumina | miseq | 3prime | poly_a | lexogen | bulk | unknown | unknown | United States | 2019-11-19 | Pharyngula | Embryo | Embryo Imprecise | All anatomical structures | |||||||||||||||||||||||||||
| 52325 | 52325 | SRR10490668 | SRX7179769 | SRS5686586 | SRP230531 | PRJNA544341 | PPAR? agonist induced disruption of dorsoventral patterning in zebrafish | PRJNA544341 | Other | The objectives of this study were to rely on mRNA sequencing to identify pathways that may be impacted following exposure to ciglitazone in the presence or absence of dorsomorphin within the first 24 h of development. | Cig 24hpf 1 S8 | strain:5D|isolate:1|dev stage:24 hpf|sex:not applicable|tissue:embryo|BioSampleModel:Model organism or animal | Cig 24hpf 1 S8 | Cig 24hpf 1 S8 | Cig 24hpf 1 S8 | QuantSeq three prime mRNA Seq Library Prep Kit FWD | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina MiniSeq | SRP230531 | Cig-24hpf-1_S8_L001_R1_001.fastq.gz | fastq | 166787278.0 | 2215546.0 | Cig 24hpf 1 S8 L001 R1 001.fastq.gz | 0:75.28 1:0 | A:61581342;C:31125964;G:37668681;T:36274594;N:136697 | 75 | 0 | 61581342 | 31125964 | 37668681 | 36274594 | 136697 | SRX7179769 | SRS5686586 | SRA998591 | University of California, Riverside|Environmental Sciences | University of California, Riverside | 1 | 0.72186 | 0.18406 | 0.83796 | 0.70527 | 75 | B | usable mapping rate | illumina | miseq | 3prime | poly_a | lexogen | bulk | unknown | unknown | United States | 2019-11-19 | Pharyngula | Embryo | Embryo Imprecise | All anatomical structures | |||||||||||||||||||||||||||
| 53838 | 53838 | SRR10393503 | SRX7093890 | SRS5606540 | SRP228416 | PRJNA563973 | High Content Screening in Zebrafish Identifies Perfluorooctanesulfonamide PFOSA as a Potent Developmental Toxicant | PRJNA563973 | Other | The objective of this study was to rely on mRNA sequencing to quantify whole transcriptome responses to perfluorooctanesulfonamide PFOSA exposure within the first 24 h of zebrafish development. | DMSO 24hpf 4 S2 | strain:5D|isolate:8|dev stage:24 hpf|sex:not applicable|tissue:embryo|BioSampleModel:Model organism or animal | DMSO 24hpf 4 S2 | DMSO 24hpf 4 S2 | DMSO 24hpf 4 S2 | QuantSeq three prime mRNA Seq Library Prep Kit FWD | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina MiniSeq | SRP228416 | DMSO-24hpf-4_S2_L001_R1_001.fastq.gz | fastq | 169544605.0 | 2252683.0 | DMSO 24hpf 4 S2 L001 R1 001.fastq.gz | 0:75.26 1:0 | A:61748050;C:32984735;G:39276677;T:35329305;N:205838 | 75 | 0 | 61748050 | 32984735 | 39276677 | 35329305 | 205838 | SRX7093890 | SRS5606540 | SRA989836 | University of California, Riverside|Environmental Sciences | University of California, Riverside | 1 | 0.69109 | 0.19681 | 0.82954 | 0.60982 | 76 | B | usable mapping rate | illumina | miseq | 3prime | poly_a | lexogen | bulk | unknown | unknown | United States | 2019-11-05 | Pharyngula | Embryo | Embryo Imprecise | All anatomical structures | |||||||||||||||||||||||||||
| 53839 | 53839 | SRR10393504 | SRX7093889 | SRS5606539 | SRP228416 | PRJNA563973 | High Content Screening in Zebrafish Identifies Perfluorooctanesulfonamide PFOSA as a Potent Developmental Toxicant | PRJNA563973 | Other | The objective of this study was to rely on mRNA sequencing to quantify whole transcriptome responses to perfluorooctanesulfonamide PFOSA exposure within the first 24 h of zebrafish development. | DMSO 24hpf 3 S1.1 | strain:5D|isolate:7|dev stage:24 hpf|sex:not applicable|tissue:embryo|BioSampleModel:Model organism or animal | DMSO 24hpf 3 S1.1 | DMSO 24hpf 3 S1.1 | DMSO 24hpf 3 S1.1 | QuantSeq three prime mRNA Seq Library Prep Kit FWD | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina MiniSeq | SRP228416 | DMSO-24hpf-3_S1_L001_R1_001.fastq.gz | fastq | 176226566.0 | 2341531.0 | DMSO 24hpf 3 S1 L001 R1 001.fastq.gz | 0:75.26 1:0 | A:62881279;C:35048395;G:42851474;T:35198274;N:247144 | 75 | 0 | 62881279 | 35048395 | 42851474 | 35198274 | 247144 | SRX7093889 | SRS5606539 | SRA989836 | University of California, Riverside|Environmental Sciences | University of California, Riverside | 1 | 0.68135 | 0.2003 | 0.83593 | 0.38242 | 76 | B | usable mapping rate | illumina | miseq | 3prime | poly_a | lexogen | bulk | unknown | unknown | United States | 2019-11-05 | Pharyngula | Embryo | Embryo Imprecise | All anatomical structures | |||||||||||||||||||||||||||
| 53840 | 53840 | SRR10393505 | SRX7093888 | SRS5606538 | SRP228416 | PRJNA563973 | High Content Screening in Zebrafish Identifies Perfluorooctanesulfonamide PFOSA as a Potent Developmental Toxicant | PRJNA563973 | Other | The objective of this study was to rely on mRNA sequencing to quantify whole transcriptome responses to perfluorooctanesulfonamide PFOSA exposure within the first 24 h of zebrafish development. | DMSO 24hpf 2 S16 | strain:5D|isolate:6|dev stage:24 hpf|sex:not applicable|tissue:embryo|BioSampleModel:Model organism or animal | DMSO 24hpf 2 S16 | DMSO 24hpf 2 S16 | DMSO 24hpf 2 S16 | QuantSeq three prime mRNA Seq Library Prep Kit FWD | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina MiniSeq | SRP228416 | DMSO-24hpf-2_S16_L001_R1_001.fastq.gz | fastq | 164476462.0 | 2183034.0 | DMSO 24hpf 2 S16 L001 R1 001.fastq.gz | 0:75.34 1:0 | A:53207677;C:34232653;G:40852867;T:36039010;N:144255 | 75 | 0 | 53207677 | 34232653 | 40852867 | 36039010 | 144255 | SRX7093888 | SRS5606538 | SRA989836 | University of California, Riverside|Environmental Sciences | University of California, Riverside | 1 | 0.76856 | 0.24847 | 0.83246 | 0.58545 | 76 | B | usable mapping rate | illumina | miseq | 3prime | poly_a | lexogen | bulk | unknown | unknown | United States | 2019-11-05 | Pharyngula | Embryo | Embryo Imprecise | All anatomical structures | |||||||||||||||||||||||||||
| 53841 | 53841 | SRR10393506 | SRX7093887 | SRS5606537 | SRP228416 | PRJNA563973 | High Content Screening in Zebrafish Identifies Perfluorooctanesulfonamide PFOSA as a Potent Developmental Toxicant | PRJNA563973 | Other | The objective of this study was to rely on mRNA sequencing to quantify whole transcriptome responses to perfluorooctanesulfonamide PFOSA exposure within the first 24 h of zebrafish development. | DMSO 24hpf 1 S15 | strain:5D|isolate:5|dev stage:24 hpf|sex:not applicable|tissue:embryo|BioSampleModel:Model organism or animal | DMSO 24hpf 1 S15 | DMSO 24hpf 1 S15 | DMSO 24hpf 1 S15 | QuantSeq three prime mRNA Seq Library Prep Kit FWD | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina MiniSeq | SRP228416 | DMSO-24hpf-1_S15_L001_R1_001.fastq.gz | fastq | 169336832.0 | 2248766.0 | DMSO 24hpf 1 S15 L001 R1 001.fastq.gz | 0:75.30 1:0 | A:59465104;C:33650141;G:42278679;T:33730782;N:212126 | 75 | 0 | 59465104 | 33650141 | 42278679 | 33730782 | 212126 | SRX7093887 | SRS5606537 | SRA989836 | University of California, Riverside|Environmental Sciences | University of California, Riverside | 1 | 0.6226 | 0.1924 | 0.84522 | 0.59451 | 74 | B | usable mapping rate | illumina | miseq | 3prime | poly_a | lexogen | bulk | unknown | unknown | United States | 2019-11-05 | Pharyngula | Embryo | Embryo Imprecise | All anatomical structures | |||||||||||||||||||||||||||
| 53844 | 53844 | SRR10393509 | SRX7093884 | SRS5606534 | SRP228416 | PRJNA563973 | High Content Screening in Zebrafish Identifies Perfluorooctanesulfonamide PFOSA as a Potent Developmental Toxicant | PRJNA563973 | Other | The objective of this study was to rely on mRNA sequencing to quantify whole transcriptome responses to perfluorooctanesulfonamide PFOSA exposure within the first 24 h of zebrafish development. | PFOSA 24hpf 4 S6 | strain:5D|isolate:16|dev stage:24 hpf|sex:not applicable|tissue:embryo|BioSampleModel:Model organism or animal | PFOSA 24hpf 4 S6 | PFOSA 24hpf 4 S6 | PFOSA 24hpf 4 S6 | QuantSeq three prime mRNA Seq Library Prep Kit FWD | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina MiniSeq | SRP228416 | PFOSA-24hpf-4_S6_L001_R1_001.fastq.gz | fastq | 159041121.0 | 2114174.0 | PFOSA 24hpf 4 S6 L001 R1 001.fastq.gz | 0:75.23 1:0 | A:55915704;C:30847758;G:41552886;T:30324805;N:399968 | 75 | 0 | 55915704 | 30847758 | 41552886 | 30324805 | 399968 | SRX7093884 | SRS5606534 | SRA989836 | University of California, Riverside|Environmental Sciences | University of California, Riverside | 1 | 0.69132 | 0.20998 | 0.86265 | 0.66863 | 75 | B | usable mapping rate | illumina | miseq | 3prime | poly_a | lexogen | bulk | unknown | unknown | United States | 2019-11-05 | Pharyngula | Embryo | Embryo Imprecise | All anatomical structures | |||||||||||||||||||||||||||
| 53845 | 53845 | SRR10393510 | SRX7093883 | SRS5606533 | SRP228416 | PRJNA563973 | High Content Screening in Zebrafish Identifies Perfluorooctanesulfonamide PFOSA as a Potent Developmental Toxicant | PRJNA563973 | Other | The objective of this study was to rely on mRNA sequencing to quantify whole transcriptome responses to perfluorooctanesulfonamide PFOSA exposure within the first 24 h of zebrafish development. | PFOSA 24hpf 3 S5 | strain:5D|isolate:15|dev stage:24 hpf|sex:not applicable|tissue:embryo|BioSampleModel:Model organism or animal | PFOSA 24hpf 3 S5 | PFOSA 24hpf 3 S5 | PFOSA 24hpf 3 S5 | QuantSeq three prime mRNA Seq Library Prep Kit FWD | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina MiniSeq | SRP228416 | PFOSA-24hpf-3_S5_L001_R1_001.fastq.gz | fastq | 176283118.0 | 2337643.0 | PFOSA 24hpf 3 S5 L001 R1 001.fastq.gz | 0:75.41 1:0 | A:60031957;C:36253921;G:44609655;T:35286711;N:100874 | 75 | 0 | 60031957 | 36253921 | 44609655 | 35286711 | 100874 | SRX7093883 | SRS5606533 | SRA989836 | University of California, Riverside|Environmental Sciences | University of California, Riverside | 1 | 0.70864 | 0.24706 | 0.85145 | 0.66729 | 75 | B | usable mapping rate | illumina | miseq | 3prime | poly_a | lexogen | bulk | unknown | unknown | United States | 2019-11-05 | Pharyngula | Embryo | Embryo Imprecise | All anatomical structures | |||||||||||||||||||||||||||
| 53846 | 53846 | SRR10393511 | SRX7093882 | SRS5606532 | SRP228416 | PRJNA563973 | High Content Screening in Zebrafish Identifies Perfluorooctanesulfonamide PFOSA as a Potent Developmental Toxicant | PRJNA563973 | Other | The objective of this study was to rely on mRNA sequencing to quantify whole transcriptome responses to perfluorooctanesulfonamide PFOSA exposure within the first 24 h of zebrafish development. | PFOSA 24hpf 2 S4 | strain:5D|isolate:14|dev stage:24 hpf|sex:not applicable|tissue:embryo|BioSampleModel:Model organism or animal | PFOSA 24hpf 2 S4 | PFOSA 24hpf 2 S4 | PFOSA 24hpf 2 S4 | QuantSeq three prime mRNA Seq Library Prep Kit FWD | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina MiniSeq | SRP228416 | PFOSA-24hpf-2_S4_L001_R1_001.fastq.gz | fastq | 147703215.0 | 1958310.0 | PFOSA 24hpf 2 S4 L001 R1 001.fastq.gz | 0:75.42 1:0 | A:47861793;C:31057983;G:38594553;T:30118087;N:70799 | 75 | 0 | 47861793 | 31057983 | 38594553 | 30118087 | 70799 | SRX7093882 | SRS5606532 | SRA989836 | University of California, Riverside|Environmental Sciences | University of California, Riverside | 1 | 0.74377 | 0.27293 | 0.85153 | 0.65156 | 76 | B | usable mapping rate | illumina | miseq | 3prime | poly_a | lexogen | bulk | unknown | unknown | United States | 2019-11-05 | Pharyngula | Embryo | Embryo Imprecise | All anatomical structures | |||||||||||||||||||||||||||
| 53847 | 53847 | SRR10393512 | SRX7093881 | SRS5606531 | SRP228416 | PRJNA563973 | High Content Screening in Zebrafish Identifies Perfluorooctanesulfonamide PFOSA as a Potent Developmental Toxicant | PRJNA563973 | Other | The objective of this study was to rely on mRNA sequencing to quantify whole transcriptome responses to perfluorooctanesulfonamide PFOSA exposure within the first 24 h of zebrafish development. | PFOSA 24hpf 1 S3 | strain:5D|isolate:13|dev stage:24 hpf|sex:not applicable|tissue:embryo|BioSampleModel:Model organism or animal | PFOSA 24hpf 1 S3 | PFOSA 24hpf 1 S3 | PFOSA 24hpf 1 S3 | QuantSeq three prime mRNA Seq Library Prep Kit FWD | RNA-Seq | TRANSCRIPTOMIC | Oligo-dT | SINGLE | ILLUMINA | Illumina MiniSeq | SRP228416 | PFOSA-24hpf-1_S3_L001_R1_001.fastq.gz | fastq | 161246238.0 | 2141247.0 | PFOSA 24hpf 1 S3 L001 R1 001.fastq.gz | 0:75.30 1:0 | A:55258914;C:33556086;G:42165900;T:30048319;N:217019 | 75 | 0 | 55258914 | 33556086 | 42165900 | 30048319 | 217019 | SRX7093881 | SRS5606531 | SRA989836 | University of California, Riverside|Environmental Sciences | University of California, Riverside | 1 | 0.66111 | 0.25796 | 0.8677 | 0.64541 | 76 | B | usable mapping rate | illumina | miseq | 3prime | poly_a | lexogen | bulk | unknown | unknown | United States | 2019-11-05 | Pharyngula | Embryo | Embryo Imprecise | All anatomical structures |
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CREATE TABLE run_metadata("run.accession" VARCHAR, "experiment.accession" VARCHAR, "sample.accession" VARCHAR, "study.accession" VARCHAR, bioproject VARCHAR, "study.title" VARCHAR, "study.alias" VARCHAR, "study.type" VARCHAR, "study.abstract" VARCHAR, "study.attributes" VARCHAR, "study.PMIDs" VARCHAR, "sample.description" VARCHAR, "sample.title" VARCHAR, "sample.alias" VARCHAR, "sample.centername" VARCHAR, "sample.attributes" VARCHAR, "GEOsample.title" VARCHAR, "GEOsample.dataprocessing" VARCHAR, "GEOsample.source" VARCHAR, "GEOsample.treatmentprotocol" VARCHAR, "GEOsample.extractprotocol" VARCHAR, "GEOsample.growthprotocol" VARCHAR, "GEOsample.characteristics" VARCHAR, "GEOsample.accession" VARCHAR, "experiment.title" VARCHAR, "experiment.alias" VARCHAR, "experiment.library_name" VARCHAR, "experiment.design_description" VARCHAR, "experiment.library_construction_protocol" VARCHAR, "experiment.attributes" VARCHAR, "experiment.library_strategy" VARCHAR, "experiment.library_source" VARCHAR, "experiment.library_selection" VARCHAR, "experiment.library_layout" VARCHAR, "experiment.platform" VARCHAR, "experiment.instrument_model" VARCHAR, "experiment.spot_descriptor" VARCHAR, "experiment.study_ref" VARCHAR, "run.title" VARCHAR, "run.attributes" VARCHAR, "run.filename" VARCHAR, "run.semantic_name" VARCHAR, "run.total_bases" DOUBLE, "run.total_spots" DOUBLE, "run.alias" VARCHAR, "run.read_lengths" VARCHAR, "run.base_counts" VARCHAR, "run.r1_length" BIGINT, "run.r2_length" BIGINT, "run.r3_length" BIGINT, "run.r4_length" BIGINT, "run.Acount" BIGINT, "run.Ccount" BIGINT, "run.Gcount" BIGINT, "run.Tcount" BIGINT, "run.Ncount" BIGINT, "run.experiment" VARCHAR, "run.pool_member" VARCHAR, "submission.accession" VARCHAR, "submission.srasource" VARCHAR, "submission.bioprojectsource" VARCHAR, "seqdetective.n_mates" BIGINT, "seqdetective.mapping_rate.mate1" DOUBLE, "seqdetective.mapping_rate.mate2" DOUBLE, "seqdetective.nofeature_rate.mate1" DOUBLE, "seqdetective.nofeature_rate.mate2" DOUBLE, "seqdetective.sparsity.mate1" DOUBLE, "seqdetective.sparsity.mate2" DOUBLE, "seqdetective.pos_strand_rate.mate1" DOUBLE, "seqdetective.pos_strand_rate.mate2" DOUBLE, "seqdetective.readlen.mate1" BIGINT, "seqdetective.readlen.mate2" BIGINT, "seqdetective.judgement.mate1" VARCHAR, "seqdetective.judgement.mate2" VARCHAR, "seqdetective.judgement.reason" VARCHAR, platform_family VARCHAR, instrument_generation VARCHAR, read_bias VARCHAR, selection_class VARCHAR, prep_kit VARCHAR, sc_or_bulk VARCHAR, tech_class VARCHAR, technology VARCHAR, tech_variant VARCHAR, "submission.bioprojectsource.country" VARCHAR, earliest_date DATE, devstage_curation VARCHAR, devstage_curation_coarse VARCHAR, tissue_curation VARCHAR, tissue_curation_coarse VARCHAR);;
CREATE INDEX idx_run_bioproject ON run_metadata(bioproject);;
CREATE INDEX idx_run_run_accession ON run_metadata("run.accession");;