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1,914 rows where devstage_curation = "Pharyngula" and experiment.library_layout = "SINGLE"

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tissue_curation >10

experiment.library_selection 10

technology 9

experiment.library_strategy 7

experiment.platform 6

experiment.library_source 2

experiment.library_layout 1

  • SINGLE · 1,914 ✖

devstage_curation_coarse 1

devstage_curation 1

  • Pharyngula · 1,914 ✖
Link rowid ▼ run.accession experiment.accession sample.accession study.accession bioproject study.title study.alias study.type study.abstract study.attributes study.PMIDs sample.description sample.title sample.alias sample.centername sample.attributes GEOsample.title GEOsample.dataprocessing GEOsample.source GEOsample.treatmentprotocol GEOsample.extractprotocol GEOsample.growthprotocol GEOsample.characteristics GEOsample.accession experiment.title experiment.alias experiment.library_name experiment.design_description experiment.library_construction_protocol experiment.attributes experiment.library_strategy experiment.library_source experiment.library_selection experiment.library_layout experiment.platform experiment.instrument_model experiment.spot_descriptor experiment.study_ref run.title run.attributes run.filename run.semantic_name run.total_bases run.total_spots run.alias run.read_lengths run.base_counts run.r1_length run.r2_length run.r3_length run.r4_length run.Acount run.Ccount run.Gcount run.Tcount run.Ncount run.experiment run.pool_member submission.accession submission.srasource submission.bioprojectsource seqdetective.n_mates seqdetective.mapping_rate.mate1 seqdetective.mapping_rate.mate2 seqdetective.nofeature_rate.mate1 seqdetective.nofeature_rate.mate2 seqdetective.sparsity.mate1 seqdetective.sparsity.mate2 seqdetective.pos_strand_rate.mate1 seqdetective.pos_strand_rate.mate2 seqdetective.readlen.mate1 seqdetective.readlen.mate2 seqdetective.judgement.mate1 seqdetective.judgement.mate2 seqdetective.judgement.reason platform_family instrument_generation read_bias selection_class prep_kit sc_or_bulk tech_class technology tech_variant submission.bioprojectsource.country earliest_date devstage_curation devstage_curation_coarse tissue_curation tissue_curation_coarse
5795 5795 ERR1698352 ERX1767860 ERS1417534 ERP018188 PRJEB16335 RNA seq of the zebrafish danio rerio brain post developmental exposure to 17a ethinylestradiol E-MTAB-5173 Transcriptome Analysis 17a ethinylestradiol EE2 is a synthetic estrogen commonly used as an active substance in oral contraceptives. It is frequently found in waste water effluent and raise concern due to its persistent nature. EE2 binds to estrogen receptors with similar affinity to oestradiol and acts as one of the most potent hormone mimics found in the environment. Estrogen is involved in many aspects of the development of the neuroendocrine system influencing both brain structure and behavior. We and others have reported a significant effect on non reproductive behaviors in adult fish and in recent studies we found that developmental exposure to EE2 resulted in an anxiogenic phenotype as adults even post a long remediation period. In this study we aim to study possible mechanisms behind the behavior alterations of zebrafish developmentally exposed to EE2 by sequencing the whole brain transcriptome. Zebrafish embryos were exposed to 0 2.14 and 7.34 ng/L EE2 from 1 day to 80 dpf post the exposure period a remediation period of 120 days followed before the fish were sampled. 3 male brains from the control group 0 ng/L and the 2.14 ng/L group were sampled and 3 female brains from the control group 0 ng/L and 7.34 ng/L were sampled. ENA FIRST PUBLIC:2017 10 02|ENA LAST UPDATE:2016 10 19|ArrayExpress:E MTAB 5173   Protocols: RNA extraction Whole brains homogenized in TriReagent according to the manufacturer 0.8 ml/sample Sigma Aldrich Germany EE2 exposure. Fish exposed to 0 2.14 or 7.34 ng/L EE2 for 80 days starting 1 dpf Ion Whole transcriptome analysis kit on RiboZero eukaryote depleted total RNA IonXpressRNA 012 up 058 12 SAMEA4518355 School of Natural Science, Technology and Environmental Studies Sodertorn University ENA first public:2017 10 02|ENA last update:2016 10 19|External Id:SAMEA4518355|INSDC center alias:School of Natural Science Technology and Environmental Studies Sodertorn University|INSDC center name:School of Natural Science Technology and Environmental Studies Sodertorn University|INSDC first public:2017 10 02T17:05:16Z|INSDC last update:2016 10 19T13:53:59Z|INSDC status:public|Submitter Id:E MTAB 5173:IonXpressRNA 012 up 058 12|age:200|broker name:ArrayExpress|common name:zebrafish|organism part:brain|sample name:E MTAB 5173:IonXpressRNA 012 up 058 12|sex:male                 Ion Torrent Proton sequencing; RNA seq of the zebrafish danio rerio brain post developmental exposure to 17α ethinylestradiol E MTAB 5173:IonXpressRNA 012 up 058 12 s IonXpressRNA 012 up 058 12 s RNA seq of the zebrafish danio rerio brain post developmental exposure to 17α ethinylestradiol RNA extraction Whole brains homogenized in TriReagent according to the manufacturer 0.8 ml/sample Sigma Aldrich Germany EE2 exposure. Fish exposed to 0 2.14 or 7.34 ng/L EE2 for 80 days starting 1 dpf Ion Whole transcriptome analysis kit on RiboZero eukaryote depleted total RNA Experimental Factor: male:sex|Experimental Factor: 17 ethinylestradiol:compound|Experimental Factor: 2.14:dose RNA-Seq TRANSCRIPTOMIC RANDOM SINGLE ION_TORRENT Ion Torrent Proton   ERP018188 Ion Torrent Proton sequencing; RNA seq of the zebrafish danio rerio brain post developmental exposure to 17α ethinylestradiol ENA FIRST PUBLIC:2017 10 02|ENA LAST UPDATE:2018 11 16 IonXpressRNA_012_up_058_12.fastq.gz fastq 2535299006.0 33956077.0 E MTAB 5173:IonXpressRNA 012 up 058 12 0:74.66 A:719061985;C:581906269;G:579932234;T:654398518;N:0 74       719061985 581906269 579932234 654398518 0 ERX1767860 ERS1417534 ERA739176 School of Natural Science, Technology and Environmental Studies Sodertorn University|European Nucleotide Archive School of Natural Science, Technology and Environmental Studies Sodertorn University|European Nucleotide Archive 1 0.7343   0.34119   0.7359   0.48406   66   B   usable mapping rate ion_torrent ion_torrent full_length random_priming ribozero bulk unknown unknown   Sweden 2016-10-19 Pharyngula Embryo Brain Nervous System
5796 5796 ERR1698351 ERX1767859 ERS1417533 ERP018188 PRJEB16335 RNA seq of the zebrafish danio rerio brain post developmental exposure to 17a ethinylestradiol E-MTAB-5173 Transcriptome Analysis 17a ethinylestradiol EE2 is a synthetic estrogen commonly used as an active substance in oral contraceptives. It is frequently found in waste water effluent and raise concern due to its persistent nature. EE2 binds to estrogen receptors with similar affinity to oestradiol and acts as one of the most potent hormone mimics found in the environment. Estrogen is involved in many aspects of the development of the neuroendocrine system influencing both brain structure and behavior. We and others have reported a significant effect on non reproductive behaviors in adult fish and in recent studies we found that developmental exposure to EE2 resulted in an anxiogenic phenotype as adults even post a long remediation period. In this study we aim to study possible mechanisms behind the behavior alterations of zebrafish developmentally exposed to EE2 by sequencing the whole brain transcriptome. Zebrafish embryos were exposed to 0 2.14 and 7.34 ng/L EE2 from 1 day to 80 dpf post the exposure period a remediation period of 120 days followed before the fish were sampled. 3 male brains from the control group 0 ng/L and the 2.14 ng/L group were sampled and 3 female brains from the control group 0 ng/L and 7.34 ng/L were sampled. ENA FIRST PUBLIC:2017 10 02|ENA LAST UPDATE:2016 10 19|ArrayExpress:E MTAB 5173   Protocols: RNA extraction Whole brains homogenized in TriReagent according to the manufacturer 0.8 ml/sample Sigma Aldrich Germany EE2 exposure. Fish exposed to 0 2.14 or 7.34 ng/L EE2 for 80 days starting 1 dpf Ion Whole transcriptome analysis kit on RiboZero eukaryote depleted total RNA IonXpressRNA 011 up 058 11 SAMEA4518354 School of Natural Science, Technology and Environmental Studies Sodertorn University ENA first public:2017 10 02|ENA last update:2016 10 19|External Id:SAMEA4518354|INSDC center alias:School of Natural Science Technology and Environmental Studies Sodertorn University|INSDC center name:School of Natural Science Technology and Environmental Studies Sodertorn University|INSDC first public:2017 10 02T17:05:16Z|INSDC last update:2016 10 19T13:53:59Z|INSDC status:public|Submitter Id:E MTAB 5173:IonXpressRNA 011 up 058 11|age:200|broker name:ArrayExpress|common name:zebrafish|organism part:brain|sample name:E MTAB 5173:IonXpressRNA 011 up 058 11|sex:male                 Ion Torrent Proton sequencing; RNA seq of the zebrafish danio rerio brain post developmental exposure to 17α ethinylestradiol E MTAB 5173:IonXpressRNA 011 up 058 11 s IonXpressRNA 011 up 058 11 s RNA seq of the zebrafish danio rerio brain post developmental exposure to 17α ethinylestradiol RNA extraction Whole brains homogenized in TriReagent according to the manufacturer 0.8 ml/sample Sigma Aldrich Germany EE2 exposure. Fish exposed to 0 2.14 or 7.34 ng/L EE2 for 80 days starting 1 dpf Ion Whole transcriptome analysis kit on RiboZero eukaryote depleted total RNA Experimental Factor: male:sex|Experimental Factor: 17 ethinylestradiol:compound|Experimental Factor: 2.14:dose RNA-Seq TRANSCRIPTOMIC RANDOM SINGLE ION_TORRENT Ion Torrent Proton   ERP018188 Ion Torrent Proton sequencing; RNA seq of the zebrafish danio rerio brain post developmental exposure to 17α ethinylestradiol ENA FIRST PUBLIC:2017 10 02|ENA LAST UPDATE:2018 11 16 IonXpressRNA_011_up_058_11.fastq.gz fastq 3400774471.0 38709756.0 E MTAB 5173:IonXpressRNA 011 up 058 11 0:87.85 A:948991439;C:793389977;G:792539547;T:865853508;N:0 87       948991439 793389977 792539547 865853508 0 ERX1767859 ERS1417533 ERA739176 School of Natural Science, Technology and Environmental Studies Sodertorn University|European Nucleotide Archive School of Natural Science, Technology and Environmental Studies Sodertorn University|European Nucleotide Archive 1 0.63968   0.27033   0.75213   0.47966   139   B   usable mapping rate ion_torrent ion_torrent full_length random_priming ribozero bulk unknown unknown   Sweden 2016-10-19 Pharyngula Embryo Brain Nervous System
5797 5797 ERR1698350 ERX1767858 ERS1417532 ERP018188 PRJEB16335 RNA seq of the zebrafish danio rerio brain post developmental exposure to 17a ethinylestradiol E-MTAB-5173 Transcriptome Analysis 17a ethinylestradiol EE2 is a synthetic estrogen commonly used as an active substance in oral contraceptives. It is frequently found in waste water effluent and raise concern due to its persistent nature. EE2 binds to estrogen receptors with similar affinity to oestradiol and acts as one of the most potent hormone mimics found in the environment. Estrogen is involved in many aspects of the development of the neuroendocrine system influencing both brain structure and behavior. We and others have reported a significant effect on non reproductive behaviors in adult fish and in recent studies we found that developmental exposure to EE2 resulted in an anxiogenic phenotype as adults even post a long remediation period. In this study we aim to study possible mechanisms behind the behavior alterations of zebrafish developmentally exposed to EE2 by sequencing the whole brain transcriptome. Zebrafish embryos were exposed to 0 2.14 and 7.34 ng/L EE2 from 1 day to 80 dpf post the exposure period a remediation period of 120 days followed before the fish were sampled. 3 male brains from the control group 0 ng/L and the 2.14 ng/L group were sampled and 3 female brains from the control group 0 ng/L and 7.34 ng/L were sampled. ENA FIRST PUBLIC:2017 10 02|ENA LAST UPDATE:2016 10 19|ArrayExpress:E MTAB 5173   Protocols: RNA extraction Whole brains homogenized in TriReagent according to the manufacturer 0.8 ml/sample Sigma Aldrich Germany EE2 exposure. Fish exposed to 0 2.14 or 7.34 ng/L EE2 for 80 days starting 1 dpf Ion Whole transcriptome analysis kit on RiboZero eukaryote depleted total RNA IonXpressRNA 010 up 058 10 SAMEA4518353 School of Natural Science, Technology and Environmental Studies Sodertorn University ENA first public:2017 10 02|ENA last update:2016 10 19|External Id:SAMEA4518353|INSDC center alias:School of Natural Science Technology and Environmental Studies Sodertorn University|INSDC center name:School of Natural Science Technology and Environmental Studies Sodertorn University|INSDC first public:2017 10 02T17:05:16Z|INSDC last update:2016 10 19T13:53:59Z|INSDC status:public|Submitter Id:E MTAB 5173:IonXpressRNA 010 up 058 10|age:200|broker name:ArrayExpress|common name:zebrafish|organism part:brain|sample name:E MTAB 5173:IonXpressRNA 010 up 058 10|sex:male                 Ion Torrent Proton sequencing; RNA seq of the zebrafish danio rerio brain post developmental exposure to 17α ethinylestradiol E MTAB 5173:IonXpressRNA 010 up 058 10 s IonXpressRNA 010 up 058 10 s RNA seq of the zebrafish danio rerio brain post developmental exposure to 17α ethinylestradiol RNA extraction Whole brains homogenized in TriReagent according to the manufacturer 0.8 ml/sample Sigma Aldrich Germany EE2 exposure. Fish exposed to 0 2.14 or 7.34 ng/L EE2 for 80 days starting 1 dpf Ion Whole transcriptome analysis kit on RiboZero eukaryote depleted total RNA Experimental Factor: male:sex|Experimental Factor: 17 ethinylestradiol:compound|Experimental Factor: 2.14:dose RNA-Seq TRANSCRIPTOMIC RANDOM SINGLE ION_TORRENT Ion Torrent Proton   ERP018188 Ion Torrent Proton sequencing; RNA seq of the zebrafish danio rerio brain post developmental exposure to 17α ethinylestradiol ENA FIRST PUBLIC:2017 10 02|ENA LAST UPDATE:2018 11 16 IonXpressRNA_010_up_058_10.fastq.gz fastq 3502667998.0 39204781.0 E MTAB 5173:IonXpressRNA 010 up 058 10 0:89.34 A:976977303;C:803330255;G:808731995;T:913628445;N:0 89       976977303 803330255 808731995 913628445 0 ERX1767858 ERS1417532 ERA739176 School of Natural Science, Technology and Environmental Studies Sodertorn University|European Nucleotide Archive School of Natural Science, Technology and Environmental Studies Sodertorn University|European Nucleotide Archive 1 0.65846   0.2815   0.74028   0.47784   86   B   usable mapping rate ion_torrent ion_torrent full_length random_priming ribozero bulk unknown unknown   Sweden 2016-10-19 Pharyngula Embryo Brain Nervous System
5798 5798 ERR1698349 ERX1767857 ERS1417531 ERP018188 PRJEB16335 RNA seq of the zebrafish danio rerio brain post developmental exposure to 17a ethinylestradiol E-MTAB-5173 Transcriptome Analysis 17a ethinylestradiol EE2 is a synthetic estrogen commonly used as an active substance in oral contraceptives. It is frequently found in waste water effluent and raise concern due to its persistent nature. EE2 binds to estrogen receptors with similar affinity to oestradiol and acts as one of the most potent hormone mimics found in the environment. Estrogen is involved in many aspects of the development of the neuroendocrine system influencing both brain structure and behavior. We and others have reported a significant effect on non reproductive behaviors in adult fish and in recent studies we found that developmental exposure to EE2 resulted in an anxiogenic phenotype as adults even post a long remediation period. In this study we aim to study possible mechanisms behind the behavior alterations of zebrafish developmentally exposed to EE2 by sequencing the whole brain transcriptome. Zebrafish embryos were exposed to 0 2.14 and 7.34 ng/L EE2 from 1 day to 80 dpf post the exposure period a remediation period of 120 days followed before the fish were sampled. 3 male brains from the control group 0 ng/L and the 2.14 ng/L group were sampled and 3 female brains from the control group 0 ng/L and 7.34 ng/L were sampled. ENA FIRST PUBLIC:2017 10 02|ENA LAST UPDATE:2016 10 19|ArrayExpress:E MTAB 5173   Protocols: RNA extraction Whole brains homogenized in TriReagent according to the manufacturer 0.8 ml/sample Sigma Aldrich Germany EE2 exposure. Fish exposed to 0 2.14 or 7.34 ng/L EE2 for 80 days starting 1 dpf Ion Whole transcriptome analysis kit on RiboZero eukaryote depleted total RNA IonXpressRNA 009 up 058 9 SAMEA4518352 School of Natural Science, Technology and Environmental Studies Sodertorn University ENA first public:2017 10 02|ENA last update:2016 10 19|External Id:SAMEA4518352|INSDC center alias:School of Natural Science Technology and Environmental Studies Sodertorn University|INSDC center name:School of Natural Science Technology and Environmental Studies Sodertorn University|INSDC first public:2017 10 02T17:05:16Z|INSDC last update:2016 10 19T13:53:59Z|INSDC status:public|Submitter Id:E MTAB 5173:IonXpressRNA 009 up 058 9|age:200|broker name:ArrayExpress|common name:zebrafish|organism part:brain|sample name:E MTAB 5173:IonXpressRNA 009 up 058 9|sex:male                 Ion Torrent Proton sequencing; RNA seq of the zebrafish danio rerio brain post developmental exposure to 17α ethinylestradiol E MTAB 5173:IonXpressRNA 009 up 058 9 s IonXpressRNA 009 up 058 9 s RNA seq of the zebrafish danio rerio brain post developmental exposure to 17α ethinylestradiol RNA extraction Whole brains homogenized in TriReagent according to the manufacturer 0.8 ml/sample Sigma Aldrich Germany EE2 exposure. Fish exposed to 0 2.14 or 7.34 ng/L EE2 for 80 days starting 1 dpf Ion Whole transcriptome analysis kit on RiboZero eukaryote depleted total RNA Experimental Factor: male:sex|Experimental Factor: n1:compound RNA-Seq TRANSCRIPTOMIC RANDOM SINGLE ION_TORRENT Ion Torrent Proton   ERP018188 Ion Torrent Proton sequencing; RNA seq of the zebrafish danio rerio brain post developmental exposure to 17α ethinylestradiol ENA FIRST PUBLIC:2017 10 02|ENA LAST UPDATE:2018 11 16 IonXpressRNA_009_up_058_9.fastq.gz fastq 3575088264.0 39745481.0 E MTAB 5173:IonXpressRNA 009 up 058 9 0:89.95 A:1011474126;C:809896078;G:807471974;T:946246086;N:0 89       1011474126 809896078 807471974 946246086 0 ERX1767857 ERS1417531 ERA739176 School of Natural Science, Technology and Environmental Studies Sodertorn University|European Nucleotide Archive School of Natural Science, Technology and Environmental Studies Sodertorn University|European Nucleotide Archive 1 0.65266   0.30274   0.75286   0.48527   94   B   usable mapping rate ion_torrent ion_torrent full_length random_priming ribozero bulk unknown unknown   Sweden 2016-10-19 Pharyngula Embryo Brain Nervous System
5799 5799 ERR1698348 ERX1767856 ERS1417530 ERP018188 PRJEB16335 RNA seq of the zebrafish danio rerio brain post developmental exposure to 17a ethinylestradiol E-MTAB-5173 Transcriptome Analysis 17a ethinylestradiol EE2 is a synthetic estrogen commonly used as an active substance in oral contraceptives. It is frequently found in waste water effluent and raise concern due to its persistent nature. EE2 binds to estrogen receptors with similar affinity to oestradiol and acts as one of the most potent hormone mimics found in the environment. Estrogen is involved in many aspects of the development of the neuroendocrine system influencing both brain structure and behavior. We and others have reported a significant effect on non reproductive behaviors in adult fish and in recent studies we found that developmental exposure to EE2 resulted in an anxiogenic phenotype as adults even post a long remediation period. In this study we aim to study possible mechanisms behind the behavior alterations of zebrafish developmentally exposed to EE2 by sequencing the whole brain transcriptome. Zebrafish embryos were exposed to 0 2.14 and 7.34 ng/L EE2 from 1 day to 80 dpf post the exposure period a remediation period of 120 days followed before the fish were sampled. 3 male brains from the control group 0 ng/L and the 2.14 ng/L group were sampled and 3 female brains from the control group 0 ng/L and 7.34 ng/L were sampled. ENA FIRST PUBLIC:2017 10 02|ENA LAST UPDATE:2016 10 19|ArrayExpress:E MTAB 5173   Protocols: RNA extraction Whole brains homogenized in TriReagent according to the manufacturer 0.8 ml/sample Sigma Aldrich Germany EE2 exposure. Fish exposed to 0 2.14 or 7.34 ng/L EE2 for 80 days starting 1 dpf Ion Whole transcriptome analysis kit on RiboZero eukaryote depleted total RNA IonXpressRNA 008 up 058 8 SAMEA4518351 School of Natural Science, Technology and Environmental Studies Sodertorn University ENA first public:2017 10 02|ENA last update:2016 10 19|External Id:SAMEA4518351|INSDC center alias:School of Natural Science Technology and Environmental Studies Sodertorn University|INSDC center name:School of Natural Science Technology and Environmental Studies Sodertorn University|INSDC first public:2017 10 02T17:05:16Z|INSDC last update:2016 10 19T13:53:59Z|INSDC status:public|Submitter Id:E MTAB 5173:IonXpressRNA 008 up 058 8|age:200|broker name:ArrayExpress|common name:zebrafish|organism part:brain|sample name:E MTAB 5173:IonXpressRNA 008 up 058 8|sex:male                 Ion Torrent Proton sequencing; RNA seq of the zebrafish danio rerio brain post developmental exposure to 17α ethinylestradiol E MTAB 5173:IonXpressRNA 008 up 058 8 s IonXpressRNA 008 up 058 8 s RNA seq of the zebrafish danio rerio brain post developmental exposure to 17α ethinylestradiol RNA extraction Whole brains homogenized in TriReagent according to the manufacturer 0.8 ml/sample Sigma Aldrich Germany EE2 exposure. Fish exposed to 0 2.14 or 7.34 ng/L EE2 for 80 days starting 1 dpf Ion Whole transcriptome analysis kit on RiboZero eukaryote depleted total RNA Experimental Factor: male:sex|Experimental Factor: n1:compound RNA-Seq TRANSCRIPTOMIC RANDOM SINGLE ION_TORRENT Ion Torrent Proton   ERP018188 Ion Torrent Proton sequencing; RNA seq of the zebrafish danio rerio brain post developmental exposure to 17α ethinylestradiol ENA FIRST PUBLIC:2017 10 02|ENA LAST UPDATE:2018 11 16 IonXpressRNA_008_up_058_8.fastq.gz fastq 3259710114.0 38530802.0 E MTAB 5173:IonXpressRNA 008 up 058 8 0:84.60 A:916053004;C:755694888;G:751125332;T:836836890;N:0 84       916053004 755694888 751125332 836836890 0 ERX1767856 ERS1417530 ERA739176 School of Natural Science, Technology and Environmental Studies Sodertorn University|European Nucleotide Archive School of Natural Science, Technology and Environmental Studies Sodertorn University|European Nucleotide Archive 1 0.70995   0.31365   0.74781   0.48846   61   B   usable mapping rate ion_torrent ion_torrent full_length random_priming ribozero bulk unknown unknown   Sweden 2016-10-19 Pharyngula Embryo Brain Nervous System
5800 5800 ERR1698347 ERX1767855 ERS1417529 ERP018188 PRJEB16335 RNA seq of the zebrafish danio rerio brain post developmental exposure to 17a ethinylestradiol E-MTAB-5173 Transcriptome Analysis 17a ethinylestradiol EE2 is a synthetic estrogen commonly used as an active substance in oral contraceptives. It is frequently found in waste water effluent and raise concern due to its persistent nature. EE2 binds to estrogen receptors with similar affinity to oestradiol and acts as one of the most potent hormone mimics found in the environment. Estrogen is involved in many aspects of the development of the neuroendocrine system influencing both brain structure and behavior. We and others have reported a significant effect on non reproductive behaviors in adult fish and in recent studies we found that developmental exposure to EE2 resulted in an anxiogenic phenotype as adults even post a long remediation period. In this study we aim to study possible mechanisms behind the behavior alterations of zebrafish developmentally exposed to EE2 by sequencing the whole brain transcriptome. Zebrafish embryos were exposed to 0 2.14 and 7.34 ng/L EE2 from 1 day to 80 dpf post the exposure period a remediation period of 120 days followed before the fish were sampled. 3 male brains from the control group 0 ng/L and the 2.14 ng/L group were sampled and 3 female brains from the control group 0 ng/L and 7.34 ng/L were sampled. ENA FIRST PUBLIC:2017 10 02|ENA LAST UPDATE:2016 10 19|ArrayExpress:E MTAB 5173   Protocols: RNA extraction Whole brains homogenized in TriReagent according to the manufacturer 0.8 ml/sample Sigma Aldrich Germany EE2 exposure. Fish exposed to 0 2.14 or 7.34 ng/L EE2 for 80 days starting 1 dpf Ion Whole transcriptome analysis kit on RiboZero eukaryote depleted total RNA IonXpressRNA 007 up 058 7 SAMEA4518350 School of Natural Science, Technology and Environmental Studies Sodertorn University ENA first public:2017 10 02|ENA last update:2016 10 19|External Id:SAMEA4518350|INSDC center alias:School of Natural Science Technology and Environmental Studies Sodertorn University|INSDC center name:School of Natural Science Technology and Environmental Studies Sodertorn University|INSDC first public:2017 10 02T17:05:16Z|INSDC last update:2016 10 19T13:53:59Z|INSDC status:public|Submitter Id:E MTAB 5173:IonXpressRNA 007 up 058 7|age:200|broker name:ArrayExpress|common name:zebrafish|organism part:brain|sample name:E MTAB 5173:IonXpressRNA 007 up 058 7|sex:male                 Ion Torrent Proton sequencing; RNA seq of the zebrafish danio rerio brain post developmental exposure to 17α ethinylestradiol E MTAB 5173:IonXpressRNA 007 up 058 7 s IonXpressRNA 007 up 058 7 s RNA seq of the zebrafish danio rerio brain post developmental exposure to 17α ethinylestradiol RNA extraction Whole brains homogenized in TriReagent according to the manufacturer 0.8 ml/sample Sigma Aldrich Germany EE2 exposure. Fish exposed to 0 2.14 or 7.34 ng/L EE2 for 80 days starting 1 dpf Ion Whole transcriptome analysis kit on RiboZero eukaryote depleted total RNA Experimental Factor: male:sex|Experimental Factor: n1:compound RNA-Seq TRANSCRIPTOMIC RANDOM SINGLE ION_TORRENT Ion Torrent Proton   ERP018188 Ion Torrent Proton sequencing; RNA seq of the zebrafish danio rerio brain post developmental exposure to 17α ethinylestradiol ENA FIRST PUBLIC:2017 10 02|ENA LAST UPDATE:2018 11 16 IonXpressRNA_007_up_058_7.fastq.gz fastq 2307842164.0 28984666.0 E MTAB 5173:IonXpressRNA 007 up 058 7 0:79.62 A:634041732;C:548565089;G:545418985;T:579816358;N:0 79       634041732 548565089 545418985 579816358 0 ERX1767855 ERS1417529 ERA739176 School of Natural Science, Technology and Environmental Studies Sodertorn University|European Nucleotide Archive School of Natural Science, Technology and Environmental Studies Sodertorn University|European Nucleotide Archive 1 0.67542   0.2817   0.74168   0.47224   37   B   usable mapping rate ion_torrent ion_torrent full_length random_priming ribozero bulk unknown unknown   Sweden 2016-10-19 Pharyngula Embryo Brain Nervous System
5801 5801 ERR1698346 ERX1767854 ERS1417528 ERP018188 PRJEB16335 RNA seq of the zebrafish danio rerio brain post developmental exposure to 17a ethinylestradiol E-MTAB-5173 Transcriptome Analysis 17a ethinylestradiol EE2 is a synthetic estrogen commonly used as an active substance in oral contraceptives. It is frequently found in waste water effluent and raise concern due to its persistent nature. EE2 binds to estrogen receptors with similar affinity to oestradiol and acts as one of the most potent hormone mimics found in the environment. Estrogen is involved in many aspects of the development of the neuroendocrine system influencing both brain structure and behavior. We and others have reported a significant effect on non reproductive behaviors in adult fish and in recent studies we found that developmental exposure to EE2 resulted in an anxiogenic phenotype as adults even post a long remediation period. In this study we aim to study possible mechanisms behind the behavior alterations of zebrafish developmentally exposed to EE2 by sequencing the whole brain transcriptome. Zebrafish embryos were exposed to 0 2.14 and 7.34 ng/L EE2 from 1 day to 80 dpf post the exposure period a remediation period of 120 days followed before the fish were sampled. 3 male brains from the control group 0 ng/L and the 2.14 ng/L group were sampled and 3 female brains from the control group 0 ng/L and 7.34 ng/L were sampled. ENA FIRST PUBLIC:2017 10 02|ENA LAST UPDATE:2016 10 19|ArrayExpress:E MTAB 5173   Protocols: RNA extraction Whole brains homogenized in TriReagent according to the manufacturer 0.8 ml/sample Sigma Aldrich Germany EE2 exposure. Fish exposed to 0 2.14 or 7.34 ng/L EE2 for 80 days starting 1 dpf Ion Whole transcriptome analysis kit on RiboZero eukaryote depleted total RNA IonXpressRNA 006 up 058 6 SAMEA4518349 School of Natural Science, Technology and Environmental Studies Sodertorn University ENA first public:2017 10 02|ENA last update:2016 10 19|External Id:SAMEA4518349|INSDC center alias:School of Natural Science Technology and Environmental Studies Sodertorn University|INSDC center name:School of Natural Science Technology and Environmental Studies Sodertorn University|INSDC first public:2017 10 02T17:05:16Z|INSDC last update:2016 10 19T13:53:59Z|INSDC status:public|Submitter Id:E MTAB 5173:IonXpressRNA 006 up 058 6|age:200|broker name:ArrayExpress|common name:zebrafish|organism part:brain|sample name:E MTAB 5173:IonXpressRNA 006 up 058 6|sex:female                 Ion Torrent Proton sequencing; RNA seq of the zebrafish danio rerio brain post developmental exposure to 17α ethinylestradiol E MTAB 5173:IonXpressRNA 006 up 058 6 s IonXpressRNA 006 up 058 6 s RNA seq of the zebrafish danio rerio brain post developmental exposure to 17α ethinylestradiol RNA extraction Whole brains homogenized in TriReagent according to the manufacturer 0.8 ml/sample Sigma Aldrich Germany EE2 exposure. Fish exposed to 0 2.14 or 7.34 ng/L EE2 for 80 days starting 1 dpf Ion Whole transcriptome analysis kit on RiboZero eukaryote depleted total RNA Experimental Factor: female:sex|Experimental Factor: 17 ethinylestradiol:compound|Experimental Factor: 7.34:dose RNA-Seq TRANSCRIPTOMIC RANDOM SINGLE ION_TORRENT Ion Torrent Proton   ERP018188 Ion Torrent Proton sequencing; RNA seq of the zebrafish danio rerio brain post developmental exposure to 17α ethinylestradiol ENA FIRST PUBLIC:2017 10 02|ENA LAST UPDATE:2018 11 16 IonXpressRNA_006_up_058_6.fastq.gz fastq 2239484857.0 27021234.0 E MTAB 5173:IonXpressRNA 006 up 058 6 0:82.88 A:637326624;C:506830056;G:503508207;T:591819970;N:0 82       637326624 506830056 503508207 591819970 0 ERX1767854 ERS1417528 ERA739176 School of Natural Science, Technology and Environmental Studies Sodertorn University|European Nucleotide Archive School of Natural Science, Technology and Environmental Studies Sodertorn University|European Nucleotide Archive 1 0.66441   0.31904   0.74905   0.47154   122   B   usable mapping rate ion_torrent ion_torrent full_length random_priming ribozero bulk unknown unknown   Sweden 2016-10-19 Pharyngula Embryo Brain Nervous System
5802 5802 ERR1698345 ERX1767853 ERS1417527 ERP018188 PRJEB16335 RNA seq of the zebrafish danio rerio brain post developmental exposure to 17a ethinylestradiol E-MTAB-5173 Transcriptome Analysis 17a ethinylestradiol EE2 is a synthetic estrogen commonly used as an active substance in oral contraceptives. It is frequently found in waste water effluent and raise concern due to its persistent nature. EE2 binds to estrogen receptors with similar affinity to oestradiol and acts as one of the most potent hormone mimics found in the environment. Estrogen is involved in many aspects of the development of the neuroendocrine system influencing both brain structure and behavior. We and others have reported a significant effect on non reproductive behaviors in adult fish and in recent studies we found that developmental exposure to EE2 resulted in an anxiogenic phenotype as adults even post a long remediation period. In this study we aim to study possible mechanisms behind the behavior alterations of zebrafish developmentally exposed to EE2 by sequencing the whole brain transcriptome. Zebrafish embryos were exposed to 0 2.14 and 7.34 ng/L EE2 from 1 day to 80 dpf post the exposure period a remediation period of 120 days followed before the fish were sampled. 3 male brains from the control group 0 ng/L and the 2.14 ng/L group were sampled and 3 female brains from the control group 0 ng/L and 7.34 ng/L were sampled. ENA FIRST PUBLIC:2017 10 02|ENA LAST UPDATE:2016 10 19|ArrayExpress:E MTAB 5173   Protocols: RNA extraction Whole brains homogenized in TriReagent according to the manufacturer 0.8 ml/sample Sigma Aldrich Germany EE2 exposure. Fish exposed to 0 2.14 or 7.34 ng/L EE2 for 80 days starting 1 dpf Ion Whole transcriptome analysis kit on RiboZero eukaryote depleted total RNA IonXpressRNA 005 up 058 5 SAMEA4518348 School of Natural Science, Technology and Environmental Studies Sodertorn University ENA first public:2017 10 02|ENA last update:2016 10 19|External Id:SAMEA4518348|INSDC center alias:School of Natural Science Technology and Environmental Studies Sodertorn University|INSDC center name:School of Natural Science Technology and Environmental Studies Sodertorn University|INSDC first public:2017 10 02T17:05:16Z|INSDC last update:2016 10 19T13:53:59Z|INSDC status:public|Submitter Id:E MTAB 5173:IonXpressRNA 005 up 058 5|age:200|broker name:ArrayExpress|common name:zebrafish|organism part:brain|sample name:E MTAB 5173:IonXpressRNA 005 up 058 5|sex:female                 Ion Torrent Proton sequencing; RNA seq of the zebrafish danio rerio brain post developmental exposure to 17α ethinylestradiol E MTAB 5173:IonXpressRNA 005 up 058 5 s IonXpressRNA 005 up 058 5 s RNA seq of the zebrafish danio rerio brain post developmental exposure to 17α ethinylestradiol RNA extraction Whole brains homogenized in TriReagent according to the manufacturer 0.8 ml/sample Sigma Aldrich Germany EE2 exposure. Fish exposed to 0 2.14 or 7.34 ng/L EE2 for 80 days starting 1 dpf Ion Whole transcriptome analysis kit on RiboZero eukaryote depleted total RNA Experimental Factor: female:sex|Experimental Factor: 17 ethinylestradiol:compound|Experimental Factor: 7.34:dose RNA-Seq TRANSCRIPTOMIC RANDOM SINGLE ION_TORRENT Ion Torrent Proton   ERP018188 Ion Torrent Proton sequencing; RNA seq of the zebrafish danio rerio brain post developmental exposure to 17α ethinylestradiol ENA FIRST PUBLIC:2017 10 02|ENA LAST UPDATE:2018 11 16 IonXpressRNA_005_up_058_5.fastq.gz fastq 2920608487.0 39573075.0 E MTAB 5173:IonXpressRNA 005 up 058 5 0:73.80 A:814851327;C:678116994;G:691911192;T:735728974;N:0 73       814851327 678116994 691911192 735728974 0 ERX1767853 ERS1417527 ERA739176 School of Natural Science, Technology and Environmental Studies Sodertorn University|European Nucleotide Archive School of Natural Science, Technology and Environmental Studies Sodertorn University|European Nucleotide Archive 1 0.60005   0.25542   0.76481   0.4886   12   B   usable mapping rate ion_torrent ion_torrent full_length random_priming ribozero bulk unknown unknown   Sweden 2016-10-19 Pharyngula Embryo Brain Nervous System
5803 5803 ERR1698344 ERX1767852 ERS1417526 ERP018188 PRJEB16335 RNA seq of the zebrafish danio rerio brain post developmental exposure to 17a ethinylestradiol E-MTAB-5173 Transcriptome Analysis 17a ethinylestradiol EE2 is a synthetic estrogen commonly used as an active substance in oral contraceptives. It is frequently found in waste water effluent and raise concern due to its persistent nature. EE2 binds to estrogen receptors with similar affinity to oestradiol and acts as one of the most potent hormone mimics found in the environment. Estrogen is involved in many aspects of the development of the neuroendocrine system influencing both brain structure and behavior. We and others have reported a significant effect on non reproductive behaviors in adult fish and in recent studies we found that developmental exposure to EE2 resulted in an anxiogenic phenotype as adults even post a long remediation period. In this study we aim to study possible mechanisms behind the behavior alterations of zebrafish developmentally exposed to EE2 by sequencing the whole brain transcriptome. Zebrafish embryos were exposed to 0 2.14 and 7.34 ng/L EE2 from 1 day to 80 dpf post the exposure period a remediation period of 120 days followed before the fish were sampled. 3 male brains from the control group 0 ng/L and the 2.14 ng/L group were sampled and 3 female brains from the control group 0 ng/L and 7.34 ng/L were sampled. ENA FIRST PUBLIC:2017 10 02|ENA LAST UPDATE:2016 10 19|ArrayExpress:E MTAB 5173   Protocols: RNA extraction Whole brains homogenized in TriReagent according to the manufacturer 0.8 ml/sample Sigma Aldrich Germany EE2 exposure. Fish exposed to 0 2.14 or 7.34 ng/L EE2 for 80 days starting 1 dpf Ion Whole transcriptome analysis kit on RiboZero eukaryote depleted total RNA IonXpressRNA 004 up 058 4 SAMEA4518347 School of Natural Science, Technology and Environmental Studies Sodertorn University ENA first public:2017 10 02|ENA last update:2016 10 19|External Id:SAMEA4518347|INSDC center alias:School of Natural Science Technology and Environmental Studies Sodertorn University|INSDC center name:School of Natural Science Technology and Environmental Studies Sodertorn University|INSDC first public:2017 10 02T17:05:16Z|INSDC last update:2016 10 19T13:53:59Z|INSDC status:public|Submitter Id:E MTAB 5173:IonXpressRNA 004 up 058 4|age:200|broker name:ArrayExpress|common name:zebrafish|organism part:brain|sample name:E MTAB 5173:IonXpressRNA 004 up 058 4|sex:female                 Ion Torrent Proton sequencing; RNA seq of the zebrafish danio rerio brain post developmental exposure to 17α ethinylestradiol E MTAB 5173:IonXpressRNA 004 up 058 4 s IonXpressRNA 004 up 058 4 s RNA seq of the zebrafish danio rerio brain post developmental exposure to 17α ethinylestradiol RNA extraction Whole brains homogenized in TriReagent according to the manufacturer 0.8 ml/sample Sigma Aldrich Germany EE2 exposure. Fish exposed to 0 2.14 or 7.34 ng/L EE2 for 80 days starting 1 dpf Ion Whole transcriptome analysis kit on RiboZero eukaryote depleted total RNA Experimental Factor: female:sex|Experimental Factor: 17 ethinylestradiol:compound|Experimental Factor: 7.34:dose RNA-Seq TRANSCRIPTOMIC RANDOM SINGLE ION_TORRENT Ion Torrent Proton   ERP018188 Ion Torrent Proton sequencing; RNA seq of the zebrafish danio rerio brain post developmental exposure to 17α ethinylestradiol ENA FIRST PUBLIC:2017 10 02|ENA LAST UPDATE:2018 11 16 IonXpressRNA_004_up_058_4.fastq.gz fastq 2989727396.0 37953454.0 E MTAB 5173:IonXpressRNA 004 up 058 4 0:78.77 A:802963810;C:722434772;G:742850530;T:721478284;N:0 78       802963810 722434772 742850530 721478284 0 ERX1767852 ERS1417526 ERA739176 School of Natural Science, Technology and Environmental Studies Sodertorn University|European Nucleotide Archive School of Natural Science, Technology and Environmental Studies Sodertorn University|European Nucleotide Archive 1 0.76402   0.32503   0.74241   0.4815   128   B   usable mapping rate ion_torrent ion_torrent full_length random_priming ribozero bulk unknown unknown   Sweden 2016-10-19 Pharyngula Embryo Brain Nervous System
5804 5804 ERR1698343 ERX1767851 ERS1417525 ERP018188 PRJEB16335 RNA seq of the zebrafish danio rerio brain post developmental exposure to 17a ethinylestradiol E-MTAB-5173 Transcriptome Analysis 17a ethinylestradiol EE2 is a synthetic estrogen commonly used as an active substance in oral contraceptives. It is frequently found in waste water effluent and raise concern due to its persistent nature. EE2 binds to estrogen receptors with similar affinity to oestradiol and acts as one of the most potent hormone mimics found in the environment. Estrogen is involved in many aspects of the development of the neuroendocrine system influencing both brain structure and behavior. We and others have reported a significant effect on non reproductive behaviors in adult fish and in recent studies we found that developmental exposure to EE2 resulted in an anxiogenic phenotype as adults even post a long remediation period. In this study we aim to study possible mechanisms behind the behavior alterations of zebrafish developmentally exposed to EE2 by sequencing the whole brain transcriptome. Zebrafish embryos were exposed to 0 2.14 and 7.34 ng/L EE2 from 1 day to 80 dpf post the exposure period a remediation period of 120 days followed before the fish were sampled. 3 male brains from the control group 0 ng/L and the 2.14 ng/L group were sampled and 3 female brains from the control group 0 ng/L and 7.34 ng/L were sampled. ENA FIRST PUBLIC:2017 10 02|ENA LAST UPDATE:2016 10 19|ArrayExpress:E MTAB 5173   Protocols: RNA extraction Whole brains homogenized in TriReagent according to the manufacturer 0.8 ml/sample Sigma Aldrich Germany EE2 exposure. Fish exposed to 0 2.14 or 7.34 ng/L EE2 for 80 days starting 1 dpf Ion Whole transcriptome analysis kit on RiboZero eukaryote depleted total RNA IonXpressRNA 003 up 058 3 SAMEA4518346 School of Natural Science, Technology and Environmental Studies Sodertorn University ENA first public:2017 10 02|ENA last update:2016 10 19|External Id:SAMEA4518346|INSDC center alias:School of Natural Science Technology and Environmental Studies Sodertorn University|INSDC center name:School of Natural Science Technology and Environmental Studies Sodertorn University|INSDC first public:2017 10 02T17:05:16Z|INSDC last update:2016 10 19T13:53:59Z|INSDC status:public|Submitter Id:E MTAB 5173:IonXpressRNA 003 up 058 3|age:200|broker name:ArrayExpress|common name:zebrafish|organism part:brain|sample name:E MTAB 5173:IonXpressRNA 003 up 058 3|sex:female                 Ion Torrent Proton sequencing; RNA seq of the zebrafish danio rerio brain post developmental exposure to 17α ethinylestradiol E MTAB 5173:IonXpressRNA 003 up 058 3 s IonXpressRNA 003 up 058 3 s RNA seq of the zebrafish danio rerio brain post developmental exposure to 17α ethinylestradiol RNA extraction Whole brains homogenized in TriReagent according to the manufacturer 0.8 ml/sample Sigma Aldrich Germany EE2 exposure. Fish exposed to 0 2.14 or 7.34 ng/L EE2 for 80 days starting 1 dpf Ion Whole transcriptome analysis kit on RiboZero eukaryote depleted total RNA Experimental Factor: female:sex|Experimental Factor: n1:compound RNA-Seq TRANSCRIPTOMIC RANDOM SINGLE ION_TORRENT Ion Torrent Proton   ERP018188 Ion Torrent Proton sequencing; RNA seq of the zebrafish danio rerio brain post developmental exposure to 17α ethinylestradiol ENA FIRST PUBLIC:2017 10 02|ENA LAST UPDATE:2018 11 16 IonXpressRNA_003_up_058_3.fastq.gz fastq 4068383115.0 41996847.0 E MTAB 5173:IonXpressRNA 003 up 058 3 0:96.87 A:1133167706;C:944306379;G:948626944;T:1042282086;N:0 96       1133167706 944306379 948626944 1042282086 0 ERX1767851 ERS1417525 ERA739176 School of Natural Science, Technology and Environmental Studies Sodertorn University|European Nucleotide Archive School of Natural Science, Technology and Environmental Studies Sodertorn University|European Nucleotide Archive 1 0.54804   0.22334   0.76353   0.49163   93   B   usable mapping rate ion_torrent ion_torrent full_length random_priming ribozero bulk unknown unknown   Sweden 2016-10-19 Pharyngula Embryo Brain Nervous System
5805 5805 ERR1698342 ERX1767850 ERS1417524 ERP018188 PRJEB16335 RNA seq of the zebrafish danio rerio brain post developmental exposure to 17a ethinylestradiol E-MTAB-5173 Transcriptome Analysis 17a ethinylestradiol EE2 is a synthetic estrogen commonly used as an active substance in oral contraceptives. It is frequently found in waste water effluent and raise concern due to its persistent nature. EE2 binds to estrogen receptors with similar affinity to oestradiol and acts as one of the most potent hormone mimics found in the environment. Estrogen is involved in many aspects of the development of the neuroendocrine system influencing both brain structure and behavior. We and others have reported a significant effect on non reproductive behaviors in adult fish and in recent studies we found that developmental exposure to EE2 resulted in an anxiogenic phenotype as adults even post a long remediation period. In this study we aim to study possible mechanisms behind the behavior alterations of zebrafish developmentally exposed to EE2 by sequencing the whole brain transcriptome. Zebrafish embryos were exposed to 0 2.14 and 7.34 ng/L EE2 from 1 day to 80 dpf post the exposure period a remediation period of 120 days followed before the fish were sampled. 3 male brains from the control group 0 ng/L and the 2.14 ng/L group were sampled and 3 female brains from the control group 0 ng/L and 7.34 ng/L were sampled. ENA FIRST PUBLIC:2017 10 02|ENA LAST UPDATE:2016 10 19|ArrayExpress:E MTAB 5173   Protocols: RNA extraction Whole brains homogenized in TriReagent according to the manufacturer 0.8 ml/sample Sigma Aldrich Germany EE2 exposure. Fish exposed to 0 2.14 or 7.34 ng/L EE2 for 80 days starting 1 dpf Ion Whole transcriptome analysis kit on RiboZero eukaryote depleted total RNA IonXpressRNA 002 up 058 2 SAMEA4518345 School of Natural Science, Technology and Environmental Studies Sodertorn University ENA first public:2017 10 02|ENA last update:2016 10 19|External Id:SAMEA4518345|INSDC center alias:School of Natural Science Technology and Environmental Studies Sodertorn University|INSDC center name:School of Natural Science Technology and Environmental Studies Sodertorn University|INSDC first public:2017 10 02T17:05:16Z|INSDC last update:2016 10 19T13:53:59Z|INSDC status:public|Submitter Id:E MTAB 5173:IonXpressRNA 002 up 058 2|age:200|broker name:ArrayExpress|common name:zebrafish|organism part:brain|sample name:E MTAB 5173:IonXpressRNA 002 up 058 2|sex:female                 Ion Torrent Proton sequencing; RNA seq of the zebrafish danio rerio brain post developmental exposure to 17α ethinylestradiol E MTAB 5173:IonXpressRNA 002 up 058 2 s IonXpressRNA 002 up 058 2 s RNA seq of the zebrafish danio rerio brain post developmental exposure to 17α ethinylestradiol RNA extraction Whole brains homogenized in TriReagent according to the manufacturer 0.8 ml/sample Sigma Aldrich Germany EE2 exposure. Fish exposed to 0 2.14 or 7.34 ng/L EE2 for 80 days starting 1 dpf Ion Whole transcriptome analysis kit on RiboZero eukaryote depleted total RNA Experimental Factor: female:sex|Experimental Factor: n1:compound RNA-Seq TRANSCRIPTOMIC RANDOM SINGLE ION_TORRENT Ion Torrent Proton   ERP018188 Ion Torrent Proton sequencing; RNA seq of the zebrafish danio rerio brain post developmental exposure to 17α ethinylestradiol ENA FIRST PUBLIC:2017 10 02|ENA LAST UPDATE:2018 11 16 IonXpressRNA_002_up_058_2.fastq.gz fastq 2679963893.0 33552723.0 E MTAB 5173:IonXpressRNA 002 up 058 2 0:79.87 A:709092431;C:635329399;G:670478210;T:665063853;N:0 79       709092431 635329399 670478210 665063853 0 ERX1767850 ERS1417524 ERA739176 School of Natural Science, Technology and Environmental Studies Sodertorn University|European Nucleotide Archive School of Natural Science, Technology and Environmental Studies Sodertorn University|European Nucleotide Archive 1 0.43283   0.18649   0.81178   0.52885   116   B   usable mapping rate ion_torrent ion_torrent full_length random_priming ribozero bulk unknown unknown   Sweden 2016-10-19 Pharyngula Embryo Brain Nervous System
5806 5806 ERR1698341 ERX1767849 ERS1417523 ERP018188 PRJEB16335 RNA seq of the zebrafish danio rerio brain post developmental exposure to 17a ethinylestradiol E-MTAB-5173 Transcriptome Analysis 17a ethinylestradiol EE2 is a synthetic estrogen commonly used as an active substance in oral contraceptives. It is frequently found in waste water effluent and raise concern due to its persistent nature. EE2 binds to estrogen receptors with similar affinity to oestradiol and acts as one of the most potent hormone mimics found in the environment. Estrogen is involved in many aspects of the development of the neuroendocrine system influencing both brain structure and behavior. We and others have reported a significant effect on non reproductive behaviors in adult fish and in recent studies we found that developmental exposure to EE2 resulted in an anxiogenic phenotype as adults even post a long remediation period. In this study we aim to study possible mechanisms behind the behavior alterations of zebrafish developmentally exposed to EE2 by sequencing the whole brain transcriptome. Zebrafish embryos were exposed to 0 2.14 and 7.34 ng/L EE2 from 1 day to 80 dpf post the exposure period a remediation period of 120 days followed before the fish were sampled. 3 male brains from the control group 0 ng/L and the 2.14 ng/L group were sampled and 3 female brains from the control group 0 ng/L and 7.34 ng/L were sampled. ENA FIRST PUBLIC:2017 10 02|ENA LAST UPDATE:2016 10 19|ArrayExpress:E MTAB 5173   Protocols: RNA extraction Whole brains homogenized in TriReagent according to the manufacturer 0.8 ml/sample Sigma Aldrich Germany EE2 exposure. Fish exposed to 0 2.14 or 7.34 ng/L EE2 for 80 days starting 1 dpf Ion Whole transcriptome analysis kit on RiboZero eukaryote depleted total RNA IonXpressRNA 001 up 058 1 SAMEA4518344 School of Natural Science, Technology and Environmental Studies Sodertorn University ENA first public:2017 10 02|ENA last update:2016 10 19|External Id:SAMEA4518344|INSDC center alias:School of Natural Science Technology and Environmental Studies Sodertorn University|INSDC center name:School of Natural Science Technology and Environmental Studies Sodertorn University|INSDC first public:2017 10 02T17:05:16Z|INSDC last update:2016 10 19T13:53:59Z|INSDC status:public|Submitter Id:E MTAB 5173:IonXpressRNA 001 up 058 1|age:200|broker name:ArrayExpress|common name:zebrafish|organism part:brain|sample name:E MTAB 5173:IonXpressRNA 001 up 058 1|sex:female                 Ion Torrent Proton sequencing; RNA seq of the zebrafish danio rerio brain post developmental exposure to 17α ethinylestradiol E MTAB 5173:IonXpressRNA 001 up 058 1 s IonXpressRNA 001 up 058 1 s RNA seq of the zebrafish danio rerio brain post developmental exposure to 17α ethinylestradiol RNA extraction Whole brains homogenized in TriReagent according to the manufacturer 0.8 ml/sample Sigma Aldrich Germany EE2 exposure. Fish exposed to 0 2.14 or 7.34 ng/L EE2 for 80 days starting 1 dpf Ion Whole transcriptome analysis kit on RiboZero eukaryote depleted total RNA Experimental Factor: female:sex|Experimental Factor: n1:compound RNA-Seq TRANSCRIPTOMIC RANDOM SINGLE ION_TORRENT Ion Torrent Proton   ERP018188 Ion Torrent Proton sequencing; RNA seq of the zebrafish danio rerio brain post developmental exposure to 17α ethinylestradiol ENA FIRST PUBLIC:2017 10 02|ENA LAST UPDATE:2018 11 16 IonXpressRNA_001_up_058_1.fastq.gz fastq 3358902499.0 39857024.0 E MTAB 5173:IonXpressRNA 001 up 058 1 0:84.27 A:933405205;C:784111716;G:780404241;T:860981337;N:0 84       933405205 784111716 780404241 860981337 0 ERX1767849 ERS1417523 ERA739176 School of Natural Science, Technology and Environmental Studies Sodertorn University|European Nucleotide Archive School of Natural Science, Technology and Environmental Studies Sodertorn University|European Nucleotide Archive 1 0.71789   0.33159   0.76619   0.514   56   B   usable mapping rate ion_torrent ion_torrent full_length random_priming ribozero bulk unknown unknown   Sweden 2016-10-19 Pharyngula Embryo Brain Nervous System
10068 10068 ERR4844843 ERX4714625 ERS5338302 ERP125162 PRJEB41393 Depletion of Foxk transcription factors causes genome wide transcriptional misregulation and developmental arrest in zebrafish embryos E-MTAB-9777 Transcriptome Analysis Foxk proteins are transcriptional regulators implicated in key biological processes such as glycolysis autophagy and cell cycle regulation among others. Here we employ targeted morpholino knockdown to deplete Foxk1 Fokx2 and Foxk2 1 proteins in developing zebrafish embryos. We demonstrate that the loss of Foxk transcription factors causes genome wide transcriptional misregulation characterised by upregulation of autophagy related genes and downregulation of cell cycle regulators. The phenotype is embryonic lethal with the majority of embryos not surviving past 24hpf. ENA FIRST PUBLIC:2020 12 01|ENA LAST UPDATE:2020 11 17   Protocols: Embryos were collected 0 hpf and incubated in 1X E3 medium 0.03% NaCl 0.005% CaCl2 0.0013% KCl 99.9557% H2O 0.008% H14MgO11S for 24 hours at 28.5°C Adult wild type AB/Tubingen Danio rerio zebrafish were bred in a 1 male:1 female ratio. 1c embryos were injected with morpholino oligonucleotides targeting foxk1 foxk2 and foxk2 1 transcripts Total RNA was purified from xxx embryos using the RNeasy Mini Kit Qiagen Valencia CA USA according to the manufacturer's instructions. mRNA Seq libraries were generated from total RNA with polyA+ selection of mRNA using the TruSeq RNA Sample Prep Kit v2 Illumina San Diego CA. Strand specific libraries were constructed using a dUTP methodology as described previously [Zhong S. et al. High Throughput Illumina Strand Specific RNA Sequencing Library Preparation. Cold Spring Harb. Protoc. 2011 940 949 2011] wt rep2 SAMEA7579966 Garvan Institute of Medical Research ENA first public:2020 12 01|ENA last update:2020 11 17|External Id:SAMEA7579966|INSDC center alias:Garvan Institute of Medical Research|INSDC center name:Garvan Institute of Medical Research|INSDC first public:2020 12 01T04:12:08Z|INSDC last update:2020 11 17T14:25:39Z|INSDC status:public|Submitter Id:E MTAB 9777:wt rep2|age:24hpf|broker name:ArrayExpress|common name:zebrafish|developmental stage:embryo stage|organism part:whole organism|sample name:E MTAB 9777:wt rep2|strain:Ab / Tubingen                 Illumina HiSeq 1500 sequencing; Depletion of Foxk transcription factors causes genome wide transcriptional misregulation and developmental arrest in zebrafish embryos E MTAB 9777:wt rep2 s wt rep2 s Depletion of Foxk transcription factors causes genome wide transcriptional misregulation and developmental arrest in zebrafish embryos Embryos were collected 0 hpf and incubated in 1X E3 medium 0.03% NaCl 0.005% CaCl2 0.0013% KCl 99.9557% H2O 0.008% H14MgO11S for 24 hours at 28.5°C Adult wild type AB/Tubingen Danio rerio zebrafish were bred in a 1 male:1 female ratio. 1c embryos were injected with morpholino oligonucleotides targeting foxk1 foxk2 and foxk2 1 transcripts Total RNA was purified from xxx embryos using the RNeasy Mini Kit Qiagen Valencia CA USA according to the manufacturer's instructions. mRNA Seq libraries were generated from total RNA with polyA+ selection of mRNA using the TruSeq RNA Sample Prep Kit v2 Illumina San Diego CA. Strand specific libraries were constructed using a dUTP methodology as described previously [Zhong S. et al. High Throughput Illumina Strand Specific RNA Sequencing Library Preparation. Cold Spring Harb. Protoc. 2011 940 949 2011] Experimental Factor: compound:n1 RNA-Seq TRANSCRIPTOMIC PolyA SINGLE ILLUMINA Illumina HiSeq 1500   ERP125162 Illumina HiSeq 1500 sequencing; Depletion of Foxk transcription factors causes genome wide transcriptional misregulation and developmental arrest in zebrafish embryos ENA FIRST PUBLIC:2020 12 01|ENA LAST UPDATE:2020 11 17 wt_rep2.fastq.gz fastq 3456340392.0 34221192.0 E MTAB 9777:wt rep2 0:101 1:0 A:850781866;C:827613239;G:837337477;T:936245388;N:4362422 101 0     850781866 827613239 837337477 936245388 4362422 ERX4714625 ERS5338302 ERA3145932 European Nucleotide Archive European Nucleotide Archive 1 0.94198   0.05681   0.69613   0.46685   101   B   usable mapping rate illumina hiseq_era unknown poly_a trueseq bulk unknown unknown   Unknown 2020-11-17 Pharyngula Embryo Whole Organism All anatomical structures
10069 10069 ERR4844842 ERX4714624 ERS5338301 ERP125162 PRJEB41393 Depletion of Foxk transcription factors causes genome wide transcriptional misregulation and developmental arrest in zebrafish embryos E-MTAB-9777 Transcriptome Analysis Foxk proteins are transcriptional regulators implicated in key biological processes such as glycolysis autophagy and cell cycle regulation among others. Here we employ targeted morpholino knockdown to deplete Foxk1 Fokx2 and Foxk2 1 proteins in developing zebrafish embryos. We demonstrate that the loss of Foxk transcription factors causes genome wide transcriptional misregulation characterised by upregulation of autophagy related genes and downregulation of cell cycle regulators. The phenotype is embryonic lethal with the majority of embryos not surviving past 24hpf. ENA FIRST PUBLIC:2020 12 01|ENA LAST UPDATE:2020 11 17   Protocols: Embryos were collected 0 hpf and incubated in 1X E3 medium 0.03% NaCl 0.005% CaCl2 0.0013% KCl 99.9557% H2O 0.008% H14MgO11S for 24 hours at 28.5°C Adult wild type AB/Tubingen Danio rerio zebrafish were bred in a 1 male:1 female ratio. 1c embryos were injected with morpholino oligonucleotides targeting foxk1 foxk2 and foxk2 1 transcripts Total RNA was purified from xxx embryos using the RNeasy Mini Kit Qiagen Valencia CA USA according to the manufacturer's instructions. mRNA Seq libraries were generated from total RNA with polyA+ selection of mRNA using the TruSeq RNA Sample Prep Kit v2 Illumina San Diego CA. Strand specific libraries were constructed using a dUTP methodology as described previously [Zhong S. et al. High Throughput Illumina Strand Specific RNA Sequencing Library Preparation. Cold Spring Harb. Protoc. 2011 940 949 2011] wt rep1 SAMEA7579965 Garvan Institute of Medical Research ENA first public:2020 12 01|ENA last update:2020 11 17|External Id:SAMEA7579965|INSDC center alias:Garvan Institute of Medical Research|INSDC center name:Garvan Institute of Medical Research|INSDC first public:2020 12 01T04:12:08Z|INSDC last update:2020 11 17T14:25:39Z|INSDC status:public|Submitter Id:E MTAB 9777:wt rep1|age:24hpf|broker name:ArrayExpress|common name:zebrafish|developmental stage:embryo stage|organism part:whole organism|sample name:E MTAB 9777:wt rep1|strain:AB / Tubingen                 Illumina HiSeq 1500 sequencing; Depletion of Foxk transcription factors causes genome wide transcriptional misregulation and developmental arrest in zebrafish embryos E MTAB 9777:wt rep1 s wt rep1 s Depletion of Foxk transcription factors causes genome wide transcriptional misregulation and developmental arrest in zebrafish embryos Embryos were collected 0 hpf and incubated in 1X E3 medium 0.03% NaCl 0.005% CaCl2 0.0013% KCl 99.9557% H2O 0.008% H14MgO11S for 24 hours at 28.5°C Adult wild type AB/Tubingen Danio rerio zebrafish were bred in a 1 male:1 female ratio. 1c embryos were injected with morpholino oligonucleotides targeting foxk1 foxk2 and foxk2 1 transcripts Total RNA was purified from xxx embryos using the RNeasy Mini Kit Qiagen Valencia CA USA according to the manufacturer's instructions. mRNA Seq libraries were generated from total RNA with polyA+ selection of mRNA using the TruSeq RNA Sample Prep Kit v2 Illumina San Diego CA. Strand specific libraries were constructed using a dUTP methodology as described previously [Zhong S. et al. High Throughput Illumina Strand Specific RNA Sequencing Library Preparation. Cold Spring Harb. Protoc. 2011 940 949 2011] Experimental Factor: compound:n1 RNA-Seq TRANSCRIPTOMIC PolyA SINGLE ILLUMINA Illumina HiSeq 1500   ERP125162 Illumina HiSeq 1500 sequencing; Depletion of Foxk transcription factors causes genome wide transcriptional misregulation and developmental arrest in zebrafish embryos ENA FIRST PUBLIC:2020 12 01|ENA LAST UPDATE:2020 11 17 wt_rep1.fastq.gz fastq 5640822932.0 55849732.0 E MTAB 9777:wt rep1 0:101 1:0 A:1394470185;C:1346058952;G:1354472388;T:1538701573;N:7119834 101 0     1394470185 1346058952 1354472388 1538701573 7119834 ERX4714624 ERS5338301 ERA3145932 European Nucleotide Archive European Nucleotide Archive 1 0.94371   0.05678   0.69664   0.47233   101   B   usable mapping rate illumina hiseq_era unknown poly_a trueseq bulk unknown unknown   Unknown 2020-11-17 Pharyngula Embryo Whole Organism All anatomical structures
10070 10070 ERR4844841 ERX4714623 ERS5338300 ERP125162 PRJEB41393 Depletion of Foxk transcription factors causes genome wide transcriptional misregulation and developmental arrest in zebrafish embryos E-MTAB-9777 Transcriptome Analysis Foxk proteins are transcriptional regulators implicated in key biological processes such as glycolysis autophagy and cell cycle regulation among others. Here we employ targeted morpholino knockdown to deplete Foxk1 Fokx2 and Foxk2 1 proteins in developing zebrafish embryos. We demonstrate that the loss of Foxk transcription factors causes genome wide transcriptional misregulation characterised by upregulation of autophagy related genes and downregulation of cell cycle regulators. The phenotype is embryonic lethal with the majority of embryos not surviving past 24hpf. ENA FIRST PUBLIC:2020 12 01|ENA LAST UPDATE:2020 11 17   Protocols: Embryos were collected 0 hpf and incubated in 1X E3 medium 0.03% NaCl 0.005% CaCl2 0.0013% KCl 99.9557% H2O 0.008% H14MgO11S for 24 hours at 28.5°C Adult wild type AB/Tubingen Danio rerio zebrafish were bred in a 1 male:1 female ratio. 1c embryos were injected with morpholino oligonucleotides targeting foxk1 foxk2 and foxk2 1 transcripts Total RNA was purified from xxx embryos using the RNeasy Mini Kit Qiagen Valencia CA USA according to the manufacturer's instructions. mRNA Seq libraries were generated from total RNA with polyA+ selection of mRNA using the TruSeq RNA Sample Prep Kit v2 Illumina San Diego CA. Strand specific libraries were constructed using a dUTP methodology as described previously [Zhong S. et al. High Throughput Illumina Strand Specific RNA Sequencing Library Preparation. Cold Spring Harb. Protoc. 2011 940 949 2011] foxk MO rep2 SAMEA7579964 Garvan Institute of Medical Research ENA first public:2020 12 01|ENA last update:2020 11 17|External Id:SAMEA7579964|INSDC center alias:Garvan Institute of Medical Research|INSDC center name:Garvan Institute of Medical Research|INSDC first public:2020 12 01T04:12:08Z|INSDC last update:2020 11 17T14:25:39Z|INSDC status:public|Submitter Id:E MTAB 9777:foxk MO rep2|age:24hpf|broker name:ArrayExpress|common name:zebrafish|developmental stage:embryo stage|organism part:whole organism|sample name:E MTAB 9777:foxk MO rep2|strain:AB / Tubingen                 Illumina HiSeq 1500 sequencing; Depletion of Foxk transcription factors causes genome wide transcriptional misregulation and developmental arrest in zebrafish embryos E MTAB 9777:foxk MO rep2 s foxk MO rep2 s Depletion of Foxk transcription factors causes genome wide transcriptional misregulation and developmental arrest in zebrafish embryos Embryos were collected 0 hpf and incubated in 1X E3 medium 0.03% NaCl 0.005% CaCl2 0.0013% KCl 99.9557% H2O 0.008% H14MgO11S for 24 hours at 28.5°C Adult wild type AB/Tubingen Danio rerio zebrafish were bred in a 1 male:1 female ratio. 1c embryos were injected with morpholino oligonucleotides targeting foxk1 foxk2 and foxk2 1 transcripts Total RNA was purified from xxx embryos using the RNeasy Mini Kit Qiagen Valencia CA USA according to the manufacturer's instructions. mRNA Seq libraries were generated from total RNA with polyA+ selection of mRNA using the TruSeq RNA Sample Prep Kit v2 Illumina San Diego CA. Strand specific libraries were constructed using a dUTP methodology as described previously [Zhong S. et al. High Throughput Illumina Strand Specific RNA Sequencing Library Preparation. Cold Spring Harb. Protoc. 2011 940 949 2011] Experimental Factor: compound:morpholino against foxk1/foxk2/foxk2 1|Experimental Factor: dose:9 RNA-Seq TRANSCRIPTOMIC PolyA SINGLE ILLUMINA Illumina HiSeq 1500   ERP125162 Illumina HiSeq 1500 sequencing; Depletion of Foxk transcription factors causes genome wide transcriptional misregulation and developmental arrest in zebrafish embryos ENA FIRST PUBLIC:2020 12 01|ENA LAST UPDATE:2020 11 17 foxk1-2_rep2.fastq.gz fastq 3550070715.0 35149215.0 E MTAB 9777:foxk MO rep2 0:101 1:0 A:901160433;C:835963324;G:840839574;T:967604789;N:4502595 101 0     901160433 835963324 840839574 967604789 4502595 ERX4714623 ERS5338300 ERA3145932 European Nucleotide Archive European Nucleotide Archive 1 0.94006   0.07603   0.69455   0.46867   101   B   usable mapping rate illumina hiseq_era unknown poly_a trueseq bulk unknown unknown   Unknown 2020-11-17 Pharyngula Embryo Whole Organism All anatomical structures
10071 10071 ERR4844840 ERX4714622 ERS5338299 ERP125162 PRJEB41393 Depletion of Foxk transcription factors causes genome wide transcriptional misregulation and developmental arrest in zebrafish embryos E-MTAB-9777 Transcriptome Analysis Foxk proteins are transcriptional regulators implicated in key biological processes such as glycolysis autophagy and cell cycle regulation among others. Here we employ targeted morpholino knockdown to deplete Foxk1 Fokx2 and Foxk2 1 proteins in developing zebrafish embryos. We demonstrate that the loss of Foxk transcription factors causes genome wide transcriptional misregulation characterised by upregulation of autophagy related genes and downregulation of cell cycle regulators. The phenotype is embryonic lethal with the majority of embryos not surviving past 24hpf. ENA FIRST PUBLIC:2020 12 01|ENA LAST UPDATE:2020 11 17   Protocols: Embryos were collected 0 hpf and incubated in 1X E3 medium 0.03% NaCl 0.005% CaCl2 0.0013% KCl 99.9557% H2O 0.008% H14MgO11S for 24 hours at 28.5°C Adult wild type AB/Tubingen Danio rerio zebrafish were bred in a 1 male:1 female ratio. 1c embryos were injected with morpholino oligonucleotides targeting foxk1 foxk2 and foxk2 1 transcripts Total RNA was purified from xxx embryos using the RNeasy Mini Kit Qiagen Valencia CA USA according to the manufacturer's instructions. mRNA Seq libraries were generated from total RNA with polyA+ selection of mRNA using the TruSeq RNA Sample Prep Kit v2 Illumina San Diego CA. Strand specific libraries were constructed using a dUTP methodology as described previously [Zhong S. et al. High Throughput Illumina Strand Specific RNA Sequencing Library Preparation. Cold Spring Harb. Protoc. 2011 940 949 2011] foxk MO rep1 SAMEA7579963 Garvan Institute of Medical Research ENA first public:2020 12 01|ENA last update:2020 11 17|External Id:SAMEA7579963|INSDC center alias:Garvan Institute of Medical Research|INSDC center name:Garvan Institute of Medical Research|INSDC first public:2020 12 01T04:12:08Z|INSDC last update:2020 11 17T14:25:39Z|INSDC status:public|Submitter Id:E MTAB 9777:foxk MO rep1|age:24hpf|broker name:ArrayExpress|common name:zebrafish|developmental stage:embryo stage|organism part:whole organism|sample name:E MTAB 9777:foxk MO rep1|strain:AB / Tubingen                 Illumina HiSeq 1500 sequencing; Depletion of Foxk transcription factors causes genome wide transcriptional misregulation and developmental arrest in zebrafish embryos E MTAB 9777:foxk MO rep1 s foxk MO rep1 s Depletion of Foxk transcription factors causes genome wide transcriptional misregulation and developmental arrest in zebrafish embryos Embryos were collected 0 hpf and incubated in 1X E3 medium 0.03% NaCl 0.005% CaCl2 0.0013% KCl 99.9557% H2O 0.008% H14MgO11S for 24 hours at 28.5°C Adult wild type AB/Tubingen Danio rerio zebrafish were bred in a 1 male:1 female ratio. 1c embryos were injected with morpholino oligonucleotides targeting foxk1 foxk2 and foxk2 1 transcripts Total RNA was purified from xxx embryos using the RNeasy Mini Kit Qiagen Valencia CA USA according to the manufacturer's instructions. mRNA Seq libraries were generated from total RNA with polyA+ selection of mRNA using the TruSeq RNA Sample Prep Kit v2 Illumina San Diego CA. Strand specific libraries were constructed using a dUTP methodology as described previously [Zhong S. et al. High Throughput Illumina Strand Specific RNA Sequencing Library Preparation. Cold Spring Harb. Protoc. 2011 940 949 2011] Experimental Factor: compound:morpholino against foxk1/foxk2/foxk2 1|Experimental Factor: dose:9 RNA-Seq TRANSCRIPTOMIC PolyA SINGLE ILLUMINA Illumina HiSeq 1500   ERP125162 Illumina HiSeq 1500 sequencing; Depletion of Foxk transcription factors causes genome wide transcriptional misregulation and developmental arrest in zebrafish embryos ENA FIRST PUBLIC:2020 12 01|ENA LAST UPDATE:2020 11 17 foxk1-2_rep1.fastq.gz fastq 3601987139.0 35663239.0 E MTAB 9777:foxk MO rep1 0:101 1:0 A:910641017;C:846039018;G:853351642;T:987409698;N:4545764 101 0     910641017 846039018 853351642 987409698 4545764 ERX4714622 ERS5338299 ERA3145932 European Nucleotide Archive European Nucleotide Archive 1 0.93784   0.08061   0.69292   0.47364   101   B   usable mapping rate illumina hiseq_era unknown poly_a trueseq bulk unknown unknown   Unknown 2020-11-17 Pharyngula Embryo Whole Organism All anatomical structures
10454 10454 ERR9267558 ERX8809981 ERS10996110 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393619 University of Heidelberg Compound:2 Ebut|Concentration:340.0 µM|ENA first public:2024 03 15|Exposure time:24 hpf Id:SAMEA13393619|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:05Z|INSDC status:public|Submitter Id:S TOXR1835:S 2 Ebut EC20 24h run3|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S 2 Ebut EC20 24h run3|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S 2 Ebut EC20 24h run3 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_2-Ebut_EC20_24h_run3.fastq.gz fastq 77386650.0 1547733.0 S TOXR1835:S 2 Ebut EC20 24h run3 r 0:50 A:15900745;C:20491362;G:18821839;T:22172078;N:626 50       15900745 20491362 18821839 22172078 626 ERX8809981 ERS10996110 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Pharyngula Embryo Cell Line Cell Line
10463 10463 ERR9267552 ERX8809975 ERS10996104 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393613 University of Heidelberg Compound:VPA|Concentration:54.0 µM|ENA first public:2024 03 15|Exposure time:24 hpf Id:SAMEA13393613|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:05Z|INSDC status:public|Submitter Id:S TOXR1835:S VPA EC20 24h run3|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S VPA EC20 24h run3|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S VPA EC20 24h run3 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_VPA_EC20_24h_run3.fastq.gz fastq 56607950.0 1132159.0 S TOXR1835:S VPA EC20 24h run3 r 0:50 A:11573141;C:14902207;G:13664100;T:16468080;N:422 50       11573141 14902207 13664100 16468080 422 ERX8809975 ERS10996104 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Pharyngula Embryo Cell Line Cell Line
10464 10464 ERR9267596 ERX8810019 ERS10996148 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393657 University of Heidelberg Compound:VPA|Concentration:54.0 µM|ENA first public:2024 03 15|Exposure time:24 hpf Id:SAMEA13393657|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:06Z|INSDC status:public|Submitter Id:S TOXR1835:S VPA EC20 24h run5|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S VPA EC20 24h run5|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S VPA EC20 24h run5 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_VPA_EC20_24h_run5.fastq.gz fastq 74744000.0 1494880.0 S TOXR1835:S VPA EC20 24h run5 r 0:50 A:15271154;C:19689007;G:18318968;T:21464272;N:599 50       15271154 19689007 18318968 21464272 599 ERX8810019 ERS10996148 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Pharyngula Embryo Cell Line Cell Line
10469 10469 ERR9267544 ERX8809967 ERS10996096 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393605 University of Heidelberg Compound:2 Mhex|Concentration:110.5 µM|ENA first public:2024 03 15|Exposure time:24 hpf Id:SAMEA13393605|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:04Z|INSDC status:public|Submitter Id:S TOXR1835:S 2 Mhex 1 2EC10 24h run2|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S 2 Mhex 1 2EC10 24h run2|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S 2 Mhex 1 2EC10 24h run2 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_2-Mhex_1-2EC10_24h_run2.fastq.gz fastq 64605400.0 1292108.0 S TOXR1835:S 2 Mhex 1 2EC10 24h run2 r 0:50 A:13413850;C:17172814;G:15822945;T:18195301;N:490 50       13413850 17172814 15822945 18195301 490 ERX8809967 ERS10996096 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Pharyngula Embryo Cell Line Cell Line
10471 10471 ERR9267531 ERX8809954 ERS10996083 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393592 University of Heidelberg Compound:VPA|Concentration:43.0 µM|ENA first public:2024 03 15|Exposure time:24 hpf Id:SAMEA13393592|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:04Z|INSDC status:public|Submitter Id:S TOXR1835:S VPA EC10 24h run2|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S VPA EC10 24h run2|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S VPA EC10 24h run2 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_VPA_EC10_24h_run2.fastq.gz fastq 54622800.0 1092456.0 S TOXR1835:S VPA EC10 24h run2 r 0:50 A:11200509;C:14326079;G:13168753;T:15927009;N:450 50       11200509 14326079 13168753 15927009 450 ERX8809954 ERS10996083 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Pharyngula Embryo Cell Line Cell Line
10472 10472 ERR9267510 ERX8809933 ERS10996062 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393571 University of Heidelberg Compound:VPA|Concentration:21.5 µM|ENA first public:2024 03 15|Exposure time:24 hpf Id:SAMEA13393571|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:03Z|INSDC status:public|Submitter Id:S TOXR1835:S VPA 1 2EC10 24h run1|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S VPA 1 2EC10 24h run1|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S VPA 1 2EC10 24h run1 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_VPA_1-2EC10_24h_run1.fastq.gz fastq 47564150.0 951283.0 S TOXR1835:S VPA 1 2EC10 24h run1 r 0:50 A:9901250;C:12587465;G:11892253;T:13182819;N:363 50       9901250 12587465 11892253 13182819 363 ERX8809933 ERS10996062 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Pharyngula Embryo Cell Line Cell Line
10473 10473 ERR9267580 ERX8810003 ERS10996132 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393641 University of Heidelberg Compound:2 Ebut|Concentration:340.0 µM|ENA first public:2024 03 15|Exposure time:24 hpf Id:SAMEA13393641|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:05Z|INSDC status:public|Submitter Id:S TOXR1835:S 2 Ebut EC20 24h run4|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S 2 Ebut EC20 24h run4|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S 2 Ebut EC20 24h run4 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_2-Ebut_EC20_24h_run4.fastq.gz fastq 70860850.0 1417217.0 S TOXR1835:S 2 Ebut EC20 24h run4 r 0:50 A:14654955;C:18637290;G:17382725;T:20185304;N:576 50       14654955 18637290 17382725 20185304 576 ERX8810003 ERS10996132 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Pharyngula Embryo Cell Line Cell Line
10478 10478 ERR9267574 ERX8809997 ERS10996126 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393635 University of Heidelberg Compound:VPA|Concentration:54.0 µM|ENA first public:2024 03 15|Exposure time:24 hpf Id:SAMEA13393635|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:05Z|INSDC status:public|Submitter Id:S TOXR1835:S VPA EC20 24h run4|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S VPA EC20 24h run4|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S VPA EC20 24h run4 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_VPA_EC20_24h_run4.fastq.gz fastq 72740300.0 1454806.0 S TOXR1835:S VPA EC20 24h run4 r 0:50 A:14859387;C:19088133;G:17571423;T:21220741;N:616 50       14859387 19088133 17571423 21220741 616 ERX8809997 ERS10996126 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Pharyngula Embryo Cell Line Cell Line
10483 10483 ERR9267608 ERX8810031 ERS10996160 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393669 University of Heidelberg Compound:2 Mhex|Concentration:253.0 µM|ENA first public:2024 03 15|Exposure time:24 hpf Id:SAMEA13393669|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:06Z|INSDC status:public|Submitter Id:S TOXR1835:S 2 Mhex EC20 24h run5|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S 2 Mhex EC20 24h run5|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S 2 Mhex EC20 24h run5 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_2-Mhex_EC20_24h_run5.fastq.gz fastq 69796100.0 1395922.0 S TOXR1835:S 2 Mhex EC20 24h run5 r 0:50 A:14281016;C:18439376;G:16990861;T:20084293;N:554 50       14281016 18439376 16990861 20084293 554 ERX8810031 ERS10996160 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Pharyngula Embryo Cell Line Cell Line
10490 10490 ERR9267532 ERX8809955 ERS10996084 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393593 University of Heidelberg Compound:VPA|Concentration:21.5 µM|ENA first public:2024 03 15|Exposure time:24 hpf Id:SAMEA13393593|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:04Z|INSDC status:public|Submitter Id:S TOXR1835:S VPA 1 2EC10 24h run2|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S VPA 1 2EC10 24h run2|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S VPA 1 2EC10 24h run2 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_VPA_1-2EC10_24h_run2.fastq.gz fastq 39722850.0 794457.0 S TOXR1835:S VPA 1 2EC10 24h run2 r 0:50 A:8097198;C:10472115;G:9592079;T:11561151;N:307 50       8097198 10472115 9592079 11561151 307 ERX8809955 ERS10996084 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Pharyngula Embryo Cell Line Cell Line
10491 10491 ERR9267538 ERX8809961 ERS10996090 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393599 University of Heidelberg Compound:2 Ebut|Concentration:139.0 µM|ENA first public:2024 03 15|Exposure time:24 hpf Id:SAMEA13393599|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:04Z|INSDC status:public|Submitter Id:S TOXR1835:S 2 Ebut 1 2EC10 24h run2|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S 2 Ebut 1 2EC10 24h run2|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S 2 Ebut 1 2EC10 24h run2 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_2-Ebut_1-2EC10_24h_run2.fastq.gz fastq 73250500.0 1465010.0 S TOXR1835:S 2 Ebut 1 2EC10 24h run2 r 0:50 A:15222334;C:19351278;G:17951984;T:20724302;N:602 50       15222334 19351278 17951984 20724302 602 ERX8809961 ERS10996090 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Pharyngula Embryo Cell Line Cell Line
10492 10492 ERR9267542 ERX8809965 ERS10996094 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393603 University of Heidelberg Compound:2 Mhex|Concentration:253.0 µM|ENA first public:2024 03 15|Exposure time:24 hpf Id:SAMEA13393603|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:04Z|INSDC status:public|Submitter Id:S TOXR1835:S 2 Mhex EC20 24h run2|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S 2 Mhex EC20 24h run2|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S 2 Mhex EC20 24h run2 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_2-Mhex_EC20_24h_run2.fastq.gz fastq 65249250.0 1304985.0 S TOXR1835:S 2 Mhex EC20 24h run2 r 0:50 A:13711582;C:17369320;G:16163352;T:18004475;N:521 50       13711582 17369320 16163352 18004475 521 ERX8809965 ERS10996094 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Pharyngula Embryo Cell Line Cell Line
10499 10499 ERR9267506 ERX8809929 ERS10996058 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393567 University of Heidelberg Compound:DMSO|Concentration:0.1 %|ENA first public:2024 03 15|Exposure time:24 hpf Id:SAMEA13393567|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:03Z|INSDC status:public|Submitter Id:S TOXR1835:S DMSO 24h run1|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S DMSO 24h run1|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S DMSO 24h run1 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_DMSO_24h_run1.fastq.gz fastq 75220500.0 1504410.0 S TOXR1835:S DMSO 24h run1 r 0:50 A:15456591;C:20474658;G:18952417;T:20336227;N:607 50       15456591 20474658 18952417 20336227 607 ERX8809929 ERS10996058 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Pharyngula Embryo Cell Line Cell Line
10503 10503 ERR9267610 ERX8810033 ERS10996162 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393671 University of Heidelberg Compound:2 Mhex|Concentration:110.5 µM|ENA first public:2024 03 15|Exposure time:24 hpf Id:SAMEA13393671|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:06Z|INSDC status:public|Submitter Id:S TOXR1835:S 2 Mhex 1 2EC10 24h run5|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S 2 Mhex 1 2EC10 24h run5|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S 2 Mhex 1 2EC10 24h run5 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_2-Mhex_1-2EC10_24h_run5.fastq.gz fastq 80281550.0 1605631.0 S TOXR1835:S 2 Mhex 1 2EC10 24h run5 r 0:50 A:16421663;C:21326310;G:19725644;T:22807321;N:612 50       16421663 21326310 19725644 22807321 612 ERX8810033 ERS10996162 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Pharyngula Embryo Cell Line Cell Line
10504 10504 ERR9267515 ERX8809938 ERS10996067 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393576 University of Heidelberg Compound:2 Ebut|Concentration:278.0 µM|ENA first public:2024 03 15|Exposure time:24 hpf Id:SAMEA13393576|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:03Z|INSDC status:public|Submitter Id:S TOXR1835:S 2 Ebut EC10 24h run1|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S 2 Ebut EC10 24h run1|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S 2 Ebut EC10 24h run1 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_2-Ebut_EC10_24h_run1.fastq.gz fastq 72324950.0 1446499.0 S TOXR1835:S 2 Ebut EC10 24h run1 r 0:50 A:14697164;C:19178040;G:17733798;T:20715349;N:599 50       14697164 19178040 17733798 20715349 599 ERX8809938 ERS10996067 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Pharyngula Embryo Cell Line Cell Line
10505 10505 ERR9267543 ERX8809966 ERS10996095 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393604 University of Heidelberg Compound:2 Mhex|Concentration:221.0 µM|ENA first public:2024 03 15|Exposure time:24 hpf Id:SAMEA13393604|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:04Z|INSDC status:public|Submitter Id:S TOXR1835:S 2 Mhex EC10 24h run2|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S 2 Mhex EC10 24h run2|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S 2 Mhex EC10 24h run2 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_2-Mhex_EC10_24h_run2.fastq.gz fastq 67215400.0 1344308.0 S TOXR1835:S 2 Mhex EC10 24h run2 r 0:50 A:14021321;C:17770179;G:16477677;T:18945728;N:495 50       14021321 17770179 16477677 18945728 495 ERX8809966 ERS10996095 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Pharyngula Embryo Cell Line Cell Line
10507 10507 ERR9267586 ERX8810009 ERS10996138 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393647 University of Heidelberg Compound:2 Mhex|Concentration:253.0 µM|ENA first public:2024 03 15|Exposure time:24 hpf Id:SAMEA13393647|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:05Z|INSDC status:public|Submitter Id:S TOXR1835:S 2 Mhex EC20 24h run4|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S 2 Mhex EC20 24h run4|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S 2 Mhex EC20 24h run4 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_2-Mhex_EC20_24h_run4.fastq.gz fastq 79071250.0 1581425.0 S TOXR1835:S 2 Mhex EC20 24h run4 r 0:50 A:16211641;C:20891506;G:19409569;T:22557862;N:672 50       16211641 20891506 19409569 22557862 672 ERX8810009 ERS10996138 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Pharyngula Embryo Cell Line Cell Line
10508 10508 ERR9267522 ERX8809945 ERS10996074 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393583 University of Heidelberg Compound:2 Mhex|Concentration:110.5 µM|ENA first public:2024 03 15|Exposure time:24 hpf Id:SAMEA13393583|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:03Z|INSDC status:public|Submitter Id:S TOXR1835:S 2 Mhex 1 2EC10 24h run1|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S 2 Mhex 1 2EC10 24h run1|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S 2 Mhex 1 2EC10 24h run1 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_2-Mhex_1-2EC10_24h_run1.fastq.gz fastq 67836050.0 1356721.0 S TOXR1835:S 2 Mhex 1 2EC10 24h run1 r 0:50 A:13891435;C:18116855;G:16597990;T:19229224;N:546 50       13891435 18116855 16597990 19229224 546 ERX8809945 ERS10996074 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Pharyngula Embryo Cell Line Cell Line
10511 10511 ERR9267509 ERX8809932 ERS10996061 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393570 University of Heidelberg Compound:VPA|Concentration:43.0 µM|ENA first public:2024 03 15|Exposure time:24 hpf Id:SAMEA13393570|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:03Z|INSDC status:public|Submitter Id:S TOXR1835:S VPA EC10 24h run1|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S VPA EC10 24h run1|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S VPA EC10 24h run1 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_VPA_EC10_24h_run1.fastq.gz fastq 59116500.0 1182330.0 S TOXR1835:S VPA EC10 24h run1 r 0:50 A:12037391;C:15821153;G:14680141;T:16577330;N:485 50       12037391 15821153 14680141 16577330 485 ERX8809932 ERS10996061 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Pharyngula Embryo Cell Line Cell Line
10512 10512 ERR9267598 ERX8810021 ERS10996150 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393659 University of Heidelberg Compound:VPA|Concentration:21.5 µM|ENA first public:2024 03 15|Exposure time:24 hpf Id:SAMEA13393659|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:06Z|INSDC status:public|Submitter Id:S TOXR1835:S VPA 1 2EC10 24h run5|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S VPA 1 2EC10 24h run5|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S VPA 1 2EC10 24h run5 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_VPA_1-2EC10_24h_run5.fastq.gz fastq 77539400.0 1550788.0 S TOXR1835:S VPA 1 2EC10 24h run5 r 0:50 A:15842699;C:20382842;G:18634109;T:22679133;N:617 50       15842699 20382842 18634109 22679133 617 ERX8810021 ERS10996150 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Pharyngula Embryo Cell Line Cell Line
10515 10515 ERR9267565 ERX8809988 ERS10996117 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393626 University of Heidelberg Compound:2 Mhex|Concentration:221.0 µM|ENA first public:2024 03 15|Exposure time:24 hpf Id:SAMEA13393626|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:05Z|INSDC status:public|Submitter Id:S TOXR1835:S 2 Mhex EC10 24h run3|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S 2 Mhex EC10 24h run3|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S 2 Mhex EC10 24h run3 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_2-Mhex_EC10_24h_run3.fastq.gz fastq 63053150.0 1261063.0 S TOXR1835:S 2 Mhex EC10 24h run3 r 0:50 A:13188112;C:16366837;G:15221999;T:18275662;N:540 50       13188112 16366837 15221999 18275662 540 ERX8809988 ERS10996117 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Pharyngula Embryo Cell Line Cell Line
10516 10516 ERR9267582 ERX8810005 ERS10996134 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393643 University of Heidelberg Compound:2 Ebut|Concentration:139.0 µM|ENA first public:2024 03 15|Exposure time:24 hpf Id:SAMEA13393643|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:05Z|INSDC status:public|Submitter Id:S TOXR1835:S 2 Ebut 1 2EC10 24h run4|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S 2 Ebut 1 2EC10 24h run4|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S 2 Ebut 1 2EC10 24h run4 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_2-Ebut_1-2EC10_24h_run4.fastq.gz fastq 72429250.0 1448585.0 S TOXR1835:S 2 Ebut 1 2EC10 24h run4 r 0:50 A:14967715;C:19065966;G:17868293;T:20526650;N:626 50       14967715 19065966 17868293 20526650 626 ERX8810005 ERS10996134 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Pharyngula Embryo Cell Line Cell Line
10518 10518 ERR9267597 ERX8810020 ERS10996149 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393658 University of Heidelberg Compound:VPA|Concentration:43.0 µM|ENA first public:2024 03 15|Exposure time:24 hpf Id:SAMEA13393658|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:06Z|INSDC status:public|Submitter Id:S TOXR1835:S VPA EC10 24h run5|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S VPA EC10 24h run5|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S VPA EC10 24h run5 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_VPA_EC10_24h_run5.fastq.gz fastq 71525900.0 1430518.0 S TOXR1835:S VPA EC10 24h run5 r 0:50 A:14677327;C:18873904;G:17392831;T:20581262;N:576 50       14677327 18873904 17392831 20581262 576 ERX8810020 ERS10996149 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Pharyngula Embryo Cell Line Cell Line
10520 10520 ERR9267560 ERX8809983 ERS10996112 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393621 University of Heidelberg Compound:2 Ebut|Concentration:139.0 µM|ENA first public:2024 03 15|Exposure time:24 hpf Id:SAMEA13393621|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:05Z|INSDC status:public|Submitter Id:S TOXR1835:S 2 Ebut 1 2EC10 24h run3|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S 2 Ebut 1 2EC10 24h run3|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S 2 Ebut 1 2EC10 24h run3 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_2-Ebut_1-2EC10_24h_run3.fastq.gz fastq 61192750.0 1223855.0 S TOXR1835:S 2 Ebut 1 2EC10 24h run3 r 0:50 A:12498110;C:16073476;G:14884074;T:17736605;N:485 50       12498110 16073476 14884074 17736605 485 ERX8809983 ERS10996112 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Pharyngula Embryo Cell Line Cell Line
10524 10524 ERR9267602 ERX8810025 ERS10996154 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393663 University of Heidelberg Compound:2 Ebut|Concentration:340.0 µM|ENA first public:2024 03 15|Exposure time:24 hpf Id:SAMEA13393663|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:06Z|INSDC status:public|Submitter Id:S TOXR1835:S 2 Ebut EC20 24h run5|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S 2 Ebut EC20 24h run5|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S 2 Ebut EC20 24h run5 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_2-Ebut_EC20_24h_run5.fastq.gz fastq 61380050.0 1227601.0 S TOXR1835:S 2 Ebut EC20 24h run5 r 0:50 A:12688864;C:16106948;G:14989705;T:17594041;N:492 50       12688864 16106948 14989705 17594041 492 ERX8810025 ERS10996154 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Pharyngula Embryo Cell Line Cell Line
10530 10530 ERR9267536 ERX8809959 ERS10996088 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393597 University of Heidelberg Compound:2 Ebut|Concentration:340.0 µM|ENA first public:2024 03 15|Exposure time:24 hpf Id:SAMEA13393597|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:04Z|INSDC status:public|Submitter Id:S TOXR1835:S 2 Ebut EC20 24h run2|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S 2 Ebut EC20 24h run2|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S 2 Ebut EC20 24h run2 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_2-Ebut_EC20_24h_run2.fastq.gz fastq 68170400.0 1363408.0 S TOXR1835:S 2 Ebut EC20 24h run2 r 0:50 A:13907721;C:18061434;G:16501968;T:19698739;N:538 50       13907721 18061434 16501968 19698739 538 ERX8809959 ERS10996088 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Pharyngula Embryo Cell Line Cell Line
10533 10533 ERR9267528 ERX8809951 ERS10996080 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393589 University of Heidelberg Compound:DMSO|Concentration:0.1 %|ENA first public:2024 03 15|Exposure time:24 hpf Id:SAMEA13393589|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:04Z|INSDC status:public|Submitter Id:S TOXR1835:S DMSO 24h run2|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S DMSO 24h run2|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S DMSO 24h run2 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_DMSO_24h_run2.fastq.gz fastq 71563850.0 1431277.0 S TOXR1835:S DMSO 24h run2 r 0:50 A:14628305;C:19001539;G:17357226;T:20576231;N:549 50       14628305 19001539 17357226 20576231 549 ERX8809951 ERS10996080 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Pharyngula Embryo Cell Line Cell Line
10535 10535 ERR9267537 ERX8809960 ERS10996089 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393598 University of Heidelberg Compound:2 Ebut|Concentration:278.0 µM|ENA first public:2024 03 15|Exposure time:24 hpf Id:SAMEA13393598|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:04Z|INSDC status:public|Submitter Id:S TOXR1835:S 2 Ebut EC10 24h run2|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S 2 Ebut EC10 24h run2|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S 2 Ebut EC10 24h run2 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_2-Ebut_EC10_24h_run2.fastq.gz fastq 68120050.0 1362401.0 S TOXR1835:S 2 Ebut EC10 24h run2 r 0:50 A:13965221;C:17986207;G:16432958;T:19735096;N:568 50       13965221 17986207 16432958 19735096 568 ERX8809960 ERS10996089 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Pharyngula Embryo Cell Line Cell Line
10536 10536 ERR9267581 ERX8810004 ERS10996133 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393642 University of Heidelberg Compound:2 Ebut|Concentration:278.0 µM|ENA first public:2024 03 15|Exposure time:24 hpf Id:SAMEA13393642|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:05Z|INSDC status:public|Submitter Id:S TOXR1835:S 2 Ebut EC10 24h run4|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S 2 Ebut EC10 24h run4|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S 2 Ebut EC10 24h run4 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_2-Ebut_EC10_24h_run4.fastq.gz fastq 71613650.0 1432273.0 S TOXR1835:S 2 Ebut EC10 24h run4 r 0:50 A:14701741;C:18830050;G:17460399;T:20620882;N:578 50       14701741 18830050 17460399 20620882 578 ERX8810004 ERS10996133 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Pharyngula Embryo Cell Line Cell Line
10540 10540 ERR9267553 ERX8809976 ERS10996105 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393614 University of Heidelberg Compound:VPA|Concentration:43.0 µM|ENA first public:2024 03 15|Exposure time:24 hpf Id:SAMEA13393614|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:05Z|INSDC status:public|Submitter Id:S TOXR1835:S VPA EC10 24h run3|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S VPA EC10 24h run3|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S VPA EC10 24h run3 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_VPA_EC10_24h_run3.fastq.gz fastq 63203400.0 1264068.0 S TOXR1835:S VPA EC10 24h run3 r 0:50 A:12952959;C:16648229;G:15464704;T:18137006;N:502 50       12952959 16648229 15464704 18137006 502 ERX8809976 ERS10996105 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Pharyngula Embryo Cell Line Cell Line
10543 10543 ERR9267603 ERX8810026 ERS10996155 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393664 University of Heidelberg Compound:2 Ebut|Concentration:278.0 µM|ENA first public:2024 03 15|Exposure time:24 hpf Id:SAMEA13393664|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:06Z|INSDC status:public|Submitter Id:S TOXR1835:S 2 Ebut EC10 24h run5|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S 2 Ebut EC10 24h run5|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S 2 Ebut EC10 24h run5 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_2-Ebut_EC10_24h_run5.fastq.gz fastq 69144200.0 1382884.0 S TOXR1835:S 2 Ebut EC10 24h run5 r 0:50 A:14342643;C:18137360;G:16893603;T:19770019;N:575 50       14342643 18137360 16893603 19770019 575 ERX8810026 ERS10996155 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Pharyngula Embryo Cell Line Cell Line
10544 10544 ERR9267516 ERX8809939 ERS10996068 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393577 University of Heidelberg Compound:2 Ebut|Concentration:139.0 µM|ENA first public:2024 03 15|Exposure time:24 hpf Id:SAMEA13393577|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:03Z|INSDC status:public|Submitter Id:S TOXR1835:S 2 Ebut 1 2EC10 24h run1|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S 2 Ebut 1 2EC10 24h run1|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S 2 Ebut 1 2EC10 24h run1 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_2-Ebut_1-2EC10_24h_run1.fastq.gz fastq 61095100.0 1221902.0 S TOXR1835:S 2 Ebut 1 2EC10 24h run1 r 0:50 A:12598869;C:16199763;G:14812809;T:17483119;N:540 50       12598869 16199763 14812809 17483119 540 ERX8809939 ERS10996068 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Pharyngula Embryo Cell Line Cell Line
10545 10545 ERR9267575 ERX8809998 ERS10996127 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393636 University of Heidelberg Compound:VPA|Concentration:43.0 µM|ENA first public:2024 03 15|Exposure time:24 hpf Id:SAMEA13393636|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:05Z|INSDC status:public|Submitter Id:S TOXR1835:S VPA EC10 24h run4|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S VPA EC10 24h run4|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S VPA EC10 24h run4 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_VPA_EC10_24h_run4.fastq.gz fastq 80315850.0 1606317.0 S TOXR1835:S VPA EC10 24h run4 r 0:50 A:16334881;C:21260448;G:19619485;T:23100382;N:654 50       16334881 21260448 19619485 23100382 654 ERX8809998 ERS10996127 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Pharyngula Embryo Cell Line Cell Line
10547 10547 ERR9267521 ERX8809944 ERS10996073 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393582 University of Heidelberg Compound:2 Mhex|Concentration:221.0 µM|ENA first public:2024 03 15|Exposure time:24 hpf Id:SAMEA13393582|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:03Z|INSDC status:public|Submitter Id:S TOXR1835:S 2 Mhex EC10 24h run1|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S 2 Mhex EC10 24h run1|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S 2 Mhex EC10 24h run1 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_2-Mhex_EC10_24h_run1.fastq.gz fastq 70789400.0 1415788.0 S TOXR1835:S 2 Mhex EC10 24h run1 r 0:50 A:14498203;C:18896328;G:17366489;T:20027834;N:546 50       14498203 18896328 17366489 20027834 546 ERX8809944 ERS10996073 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Pharyngula Embryo Cell Line Cell Line
10549 10549 ERR9267576 ERX8809999 ERS10996128 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393637 University of Heidelberg Compound:VPA|Concentration:21.5 µM|ENA first public:2024 03 15|Exposure time:24 hpf Id:SAMEA13393637|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:05Z|INSDC status:public|Submitter Id:S TOXR1835:S VPA 1 2EC10 24h run4|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S VPA 1 2EC10 24h run4|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S VPA 1 2EC10 24h run4 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_VPA_1-2EC10_24h_run4.fastq.gz fastq 76651150.0 1533023.0 S TOXR1835:S VPA 1 2EC10 24h run4 r 0:50 A:15675078;C:20183650;G:18632192;T:22159617;N:613 50       15675078 20183650 18632192 22159617 613 ERX8809999 ERS10996128 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Pharyngula Embryo Cell Line Cell Line
10550 10550 ERR9267564 ERX8809987 ERS10996116 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393625 University of Heidelberg Compound:2 Mhex|Concentration:253.0 µM|ENA first public:2024 03 15|Exposure time:24 hpf Id:SAMEA13393625|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:05Z|INSDC status:public|Submitter Id:S TOXR1835:S 2 Mhex EC20 24h run3|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S 2 Mhex EC20 24h run3|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S 2 Mhex EC20 24h run3 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_2-Mhex_EC20_24h_run3.fastq.gz fastq 71554550.0 1431091.0 S TOXR1835:S 2 Mhex EC20 24h run3 r 0:50 A:14691978;C:18921635;G:17433619;T:20506704;N:614 50       14691978 18921635 17433619 20506704 614 ERX8809987 ERS10996116 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Pharyngula Embryo Cell Line Cell Line
10552 10552 ERR9267508 ERX8809931 ERS10996060 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393569 University of Heidelberg Compound:VPA|Concentration:54.0 µM|ENA first public:2024 03 15|Exposure time:24 hpf Id:SAMEA13393569|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:03Z|INSDC status:public|Submitter Id:S TOXR1835:S VPA EC20 24h run1|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S VPA EC20 24h run1|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S VPA EC20 24h run1 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_VPA_EC20_24h_run1.fastq.gz fastq 58109550.0 1162191.0 S TOXR1835:S VPA EC20 24h run1 r 0:50 A:11912533;C:15535929;G:14498424;T:16162192;N:472 50       11912533 15535929 14498424 16162192 472 ERX8809931 ERS10996060 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Pharyngula Embryo Cell Line Cell Line
10555 10555 ERR9267514 ERX8809937 ERS10996066 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393575 University of Heidelberg Compound:2 Ebut|Concentration:340.0 µM|ENA first public:2024 03 15|Exposure time:24 hpf Id:SAMEA13393575|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:03Z|INSDC status:public|Submitter Id:S TOXR1835:S 2 Ebut EC20 24h run1|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S 2 Ebut EC20 24h run1|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S 2 Ebut EC20 24h run1 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_2-Ebut_EC20_24h_run1.fastq.gz fastq 74971250.0 1499425.0 S TOXR1835:S 2 Ebut EC20 24h run1 r 0:50 A:15584309;C:19886247;G:18463203;T:21036851;N:640 50       15584309 19886247 18463203 21036851 640 ERX8809937 ERS10996066 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Pharyngula Embryo Cell Line Cell Line
10556 10556 ERR9267520 ERX8809943 ERS10996072 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393581 University of Heidelberg Compound:2 Mhex|Concentration:253.0 µM|ENA first public:2024 03 15|Exposure time:24 hpf Id:SAMEA13393581|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:03Z|INSDC status:public|Submitter Id:S TOXR1835:S 2 Mhex EC20 24h run1|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S 2 Mhex EC20 24h run1|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S 2 Mhex EC20 24h run1 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_2-Mhex_EC20_24h_run1.fastq.gz fastq 70012800.0 1400256.0 S TOXR1835:S 2 Mhex EC20 24h run1 r 0:50 A:14373300;C:18657161;G:17190667;T:19790999;N:673 50       14373300 18657161 17190667 19790999 673 ERX8809943 ERS10996072 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Pharyngula Embryo Cell Line Cell Line
10559 10559 ERR9267588 ERX8810011 ERS10996140 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393649 University of Heidelberg Compound:2 Mhex|Concentration:110.5 µM|ENA first public:2024 03 15|Exposure time:24 hpf Id:SAMEA13393649|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:06Z|INSDC status:public|Submitter Id:S TOXR1835:S 2 Mhex 1 2EC10 24h run4|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S 2 Mhex 1 2EC10 24h run4|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S 2 Mhex 1 2EC10 24h run4 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_2-Mhex_1-2EC10_24h_run4.fastq.gz fastq 75761200.0 1515224.0 S TOXR1835:S 2 Mhex 1 2EC10 24h run4 r 0:50 A:15474561;C:20162131;G:18671084;T:21452813;N:611 50       15474561 20162131 18671084 21452813 611 ERX8810011 ERS10996140 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Pharyngula Embryo Cell Line Cell Line
10563 10563 ERR9267550 ERX8809973 ERS10996102 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393611 University of Heidelberg Compound:DMSO|Concentration:0.1 %|ENA first public:2024 03 15|Exposure time:24 hpf Id:SAMEA13393611|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:04Z|INSDC status:public|Submitter Id:S TOXR1835:S DMSO 24h run3|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S DMSO 24h run3|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S DMSO 24h run3 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_DMSO_24h_run3.fastq.gz fastq 69322100.0 1386442.0 S TOXR1835:S DMSO 24h run3 r 0:50 A:14073222;C:18386696;G:16912301;T:19949313;N:568 50       14073222 18386696 16912301 19949313 568 ERX8809973 ERS10996102 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Pharyngula Embryo Cell Line Cell Line
10567 10567 ERR9267609 ERX8810032 ERS10996161 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393670 University of Heidelberg Compound:2 Mhex|Concentration:221.0 µM|ENA first public:2024 03 15|Exposure time:24 hpf Id:SAMEA13393670|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:06Z|INSDC status:public|Submitter Id:S TOXR1835:S 2 Mhex EC10 24h run5|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S 2 Mhex EC10 24h run5|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S 2 Mhex EC10 24h run5 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_2-Mhex_EC10_24h_run5.fastq.gz fastq 72040150.0 1440803.0 S TOXR1835:S 2 Mhex EC10 24h run5 r 0:50 A:14750518;C:19029981;G:17564059;T:20695002;N:590 50       14750518 19029981 17564059 20695002 590 ERX8810032 ERS10996161 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Pharyngula Embryo Cell Line Cell Line
10570 10570 ERR9267559 ERX8809982 ERS10996111 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393620 University of Heidelberg Compound:2 Ebut|Concentration:278.0 µM|ENA first public:2024 03 15|Exposure time:24 hpf Id:SAMEA13393620|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:05Z|INSDC status:public|Submitter Id:S TOXR1835:S 2 Ebut EC10 24h run3|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S 2 Ebut EC10 24h run3|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S 2 Ebut EC10 24h run3 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_2-Ebut_EC10_24h_run3.fastq.gz fastq 73941300.0 1478826.0 S TOXR1835:S 2 Ebut EC10 24h run3 r 0:50 A:15366120;C:19364001;G:18011340;T:21199239;N:600 50       15366120 19364001 18011340 21199239 600 ERX8809982 ERS10996111 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Pharyngula Embryo Cell Line Cell Line
10575 10575 ERR9267530 ERX8809953 ERS10996082 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393591 University of Heidelberg Compound:VPA|Concentration:54.0 µM|ENA first public:2024 03 15|Exposure time:24 hpf Id:SAMEA13393591|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:04Z|INSDC status:public|Submitter Id:S TOXR1835:S VPA EC20 24h run2|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S VPA EC20 24h run2|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S VPA EC20 24h run2 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_VPA_EC20_24h_run2.fastq.gz fastq 55846150.0 1116923.0 S TOXR1835:S VPA EC20 24h run2 r 0:50 A:11487115;C:14682007;G:13493343;T:16183215;N:470 50       11487115 14682007 13493343 16183215 470 ERX8809953 ERS10996082 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Pharyngula Embryo Cell Line Cell Line
10577 10577 ERR9267504 ERX8809927 ERS10996056 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393565 University of Heidelberg Compound:H2O|Concentration:nan nan|ENA first public:2024 03 15|Exposure time:24 hpf Id:SAMEA13393565|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:03Z|INSDC status:public|Submitter Id:S TOXR1835:S NK 24h run1|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S NK 24h run1|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S NK 24h run1 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_NK_24h_run1.fastq.gz fastq 72467700.0 1449354.0 S TOXR1835:S NK 24h run1 r 0:50 A:14870474;C:19586715;G:18148273;T:19861616;N:622 50       14870474 19586715 18148273 19861616 622 ERX8809927 ERS10996056 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Pharyngula Embryo Cell Line Cell Line
10584 10584 ERR9267548 ERX8809971 ERS10996100 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393609 University of Heidelberg Compound:H2O|Concentration:nan nan|ENA first public:2024 03 15|Exposure time:24 hpf Id:SAMEA13393609|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:04Z|INSDC status:public|Submitter Id:S TOXR1835:S NK 24h run3|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S NK 24h run3|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S NK 24h run3 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_NK_24h_run3.fastq.gz fastq 71899550.0 1437991.0 S TOXR1835:S NK 24h run3 r 0:50 A:14635153;C:19099594;G:17485499;T:20678684;N:620 50       14635153 19099594 17485499 20678684 620 ERX8809971 ERS10996100 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Pharyngula Embryo Cell Line Cell Line
10594 10594 ERR9267566 ERX8809989 ERS10996118 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393627 University of Heidelberg Compound:2 Mhex|Concentration:110.5 µM|ENA first public:2024 03 15|Exposure time:24 hpf Id:SAMEA13393627|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:05Z|INSDC status:public|Submitter Id:S TOXR1835:S 2 Mhex 1 2EC10 24h run3|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S 2 Mhex 1 2EC10 24h run3|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S 2 Mhex 1 2EC10 24h run3 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_2-Mhex_1-2EC10_24h_run3.fastq.gz fastq 70350050.0 1407001.0 S TOXR1835:S 2 Mhex 1 2EC10 24h run3 r 0:50 A:14279692;C:18591291;G:17172710;T:20305828;N:529 50       14279692 18591291 17172710 20305828 529 ERX8809989 ERS10996118 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Pharyngula Embryo Cell Line Cell Line
10597 10597 ERR9267526 ERX8809949 ERS10996078 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393587 University of Heidelberg Compound:H2O|Concentration:nan nan|ENA first public:2024 03 15|Exposure time:24 hpf Id:SAMEA13393587|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:03Z|INSDC status:public|Submitter Id:S TOXR1835:S NK 24h run2|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S NK 24h run2|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S NK 24h run2 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_NK_24h_run2.fastq.gz fastq 75912300.0 1518246.0 S TOXR1835:S NK 24h run2 r 0:50 A:15600935;C:20232557;G:18734139;T:21344046;N:623 50       15600935 20232557 18734139 21344046 623 ERX8809949 ERS10996078 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Pharyngula Embryo Cell Line Cell Line
10598 10598 ERR9267570 ERX8809993 ERS10996122 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393631 University of Heidelberg Compound:H2O|Concentration:nan nan|ENA first public:2024 03 15|Exposure time:24 hpf Id:SAMEA13393631|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:05Z|INSDC status:public|Submitter Id:S TOXR1835:S NK 24h run4|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S NK 24h run4|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S NK 24h run4 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_NK_24h_run4.fastq.gz fastq 71027750.0 1420555.0 S TOXR1835:S NK 24h run4 r 0:50 A:14611810;C:18688407;G:17316162;T:20410790;N:581 50       14611810 18688407 17316162 20410790 581 ERX8809993 ERS10996122 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Pharyngula Embryo Cell Line Cell Line
10601 10601 ERR9267604 ERX8810027 ERS10996156 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       S TOXR1835:S 2 Ebut 1 2EC10 24h run5   Treatment:repeated dose|Treatment scheme:daily|organism:Danio rerio|cell line:null|Compound:2 Ebut|scientific name:Danio rerio|Concentration:139.0 µM|Exposure time:24 hpf name:zebrafish|ENA LAST UPDATE:2022 03 15                 Illumina HiSeq 2500 sequencing S TOXR1835:S 2 Ebut 1 2EC10 24h run5 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_2-Ebut_1-2EC10_24h_run5.fastq.gz fastq 72204950.0 1444099.0 S TOXR1835:S 2 Ebut 1 2EC10 24h run5 r 0:50 A:14929489;C:18973892;G:17613744;T:20687217;N:608 50       14929489 18973892 17613744 20687217 608 ERX8810027 ERS10996156 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Pharyngula Embryo Cell Line Cell Line
10612 10612 ERR9267594 ERX8810017 ERS10996146 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       S TOXR1835:S DMSO 24h run5   Treatment:repeated dose|Treatment scheme:daily|organism:Danio rerio|cell line:null|Compound:DMSO|scientific name:Danio rerio|Concentration:0.1 %|Exposure time:24 hpf name:zebrafish|ENA LAST UPDATE:2022 03 15                 Illumina HiSeq 2500 sequencing S TOXR1835:S DMSO 24h run5 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_DMSO_24h_run5.fastq.gz fastq 723600.0 14472.0 S TOXR1835:S DMSO 24h run5 r 0:50 A:201971;C:183025;G:152695;T:185902;N:7 50       201971 183025 152695 185902 7 ERX8810017 ERS10996146 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Pharyngula Embryo Cell Line Cell Line
10614 10614 ERR9267554 ERX8809977 ERS10996106 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       S TOXR1835:S VPA 1 2EC10 24h run3   Treatment:repeated dose|Treatment scheme:daily|organism:Danio rerio|cell line:null|Compound:VPA|scientific name:Danio rerio|Concentration:21.5 µM|Exposure time:24 hpf name:zebrafish|ENA LAST UPDATE:2022 03 15                 Illumina HiSeq 2500 sequencing S TOXR1835:S VPA 1 2EC10 24h run3 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_VPA_1-2EC10_24h_run3.fastq.gz fastq 70791200.0 1415824.0 S TOXR1835:S VPA 1 2EC10 24h run3 r 0:50 A:14404772;C:18876486;G:17539965;T:19969386;N:591 50       14404772 18876486 17539965 19969386 591 ERX8809977 ERS10996106 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Pharyngula Embryo Cell Line Cell Line
10615 10615 ERR9267587 ERX8810010 ERS10996139 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       S TOXR1835:S 2 Mhex EC10 24h run4   Treatment:repeated dose|Treatment scheme:daily|organism:Danio rerio|cell line:null|Compound:2 Mhex|scientific name:Danio rerio|Concentration:221.0 µM|Exposure time:24 hpf name:zebrafish|ENA LAST UPDATE:2022 03 15                 Illumina HiSeq 2500 sequencing S TOXR1835:S 2 Mhex EC10 24h run4 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_2-Mhex_EC10_24h_run4.fastq.gz fastq 80041600.0 1600832.0 S TOXR1835:S 2 Mhex EC10 24h run4 r 0:50 A:16407828;C:21211478;G:19553748;T:22867924;N:622 50       16407828 21211478 19553748 22867924 622 ERX8810010 ERS10996139 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Pharyngula Embryo Cell Line Cell Line
10617 10617 ERR9267592 ERX8810015 ERS10996144 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393653 University of Heidelberg Compound:H2O|Concentration:nan nan|ENA first public:2024 03 15|Exposure time:24 hpf Id:SAMEA13393653|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:06Z|INSDC status:public|Submitter Id:S TOXR1835:S NK 24h run5|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S NK 24h run5|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S NK 24h run5 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_NK_24h_run5.fastq.gz fastq 71628950.0 1432579.0 S TOXR1835:S NK 24h run5 r 0:50 A:14738445;C:18905171;G:17412936;T:20571806;N:592 50       14738445 18905171 17412936 20571806 592 ERX8810015 ERS10996144 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Pharyngula Embryo Cell Line Cell Line
10623 10623 ERR9267572 ERX8809995 ERS10996124 ERP136287 PRJEB51640 CS2 UHEI DART 96 120h raw data EUT080 S-TOXR1835 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13393633 University of Heidelberg Compound:DMSO|Concentration:0.1 %|ENA first public:2024 03 15|Exposure time:24 hpf Id:SAMEA13393633|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:42:05Z|INSDC status:public|Submitter Id:S TOXR1835:S DMSO 24h run4|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1835:S DMSO 24h run4|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1835:S DMSO 24h run4 e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136287 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_DMSO_24h_run4.fastq.gz fastq 76571400.0 1531428.0 S TOXR1835:S DMSO 24h run4 r 0:50 A:15729570;C:20288415;G:18672527;T:21880297;N:591 50       15729570 20288415 18672527 21880297 591 ERX8809995 ERS10996124 ERA10091655 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Pharyngula Embryo Cell Line Cell Line
10624 10624 ERR9269919 ERX8812316 ERS10998382 ERP136288 PRJEB51641 CS2 UHEI DART 96 120h raw data EUT053 S-TOXR1832 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13395728 University of Heidelberg Compound:VPA|Concentration:2.0 µM|ENA first public:2024 03 15|Exposure time:24 hpf|External Id:SAMEA13395728|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:53:12Z|INSDC status:public|Submitter Id:S TOXR1832:S 24hpf VPA2 UHEI|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1832:S 24hpf VPA2 UHEI|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1832:S 24hpf VPA2 UHEI e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136288 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_24hpf_VPA2_UHEI.fastq.gz fastq 59344750.0 1186895.0 S TOXR1832:S 24hpf VPA2 UHEI r 0:50 A:12361540;C:15343560;G:14226695;T:17405034;N:7921 50       12361540 15343560 14226695 17405034 7921 ERX8812316 ERS10998382 ERA10091710 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Pharyngula Embryo Cell Line Cell Line
10625 10625 ERR9269913 ERX8812310 ERS10998376 ERP136288 PRJEB51641 CS2 UHEI DART 96 120h raw data EUT053 S-TOXR1832 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13395722 University of Heidelberg Compound:VPA|Concentration:50.0 µM|ENA first public:2024 03 15|Exposure time:24 hpf|External Id:SAMEA13395722|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:53:12Z|INSDC status:public|Submitter Id:S TOXR1832:S 24hpf VPA50 UHEI|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1832:S 24hpf VPA50 UHEI|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1832:S 24hpf VPA50 UHEI e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136288 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_24hpf_VPA50_UHEI.fastq.gz fastq 87495250.0 1749905.0 S TOXR1832:S 24hpf VPA50 UHEI r 0:50 A:18298773;C:22233747;G:20342014;T:26609135;N:11581 50       18298773 22233747 20342014 26609135 11581 ERX8812310 ERS10998376 ERA10091710 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Pharyngula Embryo Cell Line Cell Line
10628 10628 ERR9269907 ERX8812304 ERS10998369 ERP136288 PRJEB51641 CS2 UHEI DART 96 120h raw data EUT053 S-TOXR1832 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13395716 University of Heidelberg Compound:VPA|Concentration:200.0 µM|ENA first public:2024 03 15|Exposure time:24 hpf|External Id:SAMEA13395716|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:53:12Z|INSDC status:public|Submitter Id:S TOXR1832:S 24hpf VPA200 UHEI|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1832:S 24hpf VPA200 UHEI|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1832:S 24hpf VPA200 UHEI e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136288 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_24hpf_VPA200_UHEI.fastq.gz fastq 98702050.0 1974041.0 S TOXR1832:S 24hpf VPA200 UHEI r 0:50 A:20978758;C:25656186;G:24386413;T:27667317;N:13376 50       20978758 25656186 24386413 27667317 13376 ERX8812304 ERS10998369 ERA10091710 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Pharyngula Embryo Cell Line Cell Line
10630 10630 ERR9269904 ERX8812301 ERS10998366 ERP136288 PRJEB51641 CS2 UHEI DART 96 120h raw data EUT053 S-TOXR1832 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13395713 University of Heidelberg Compound:DMSO|Concentration:0.1 %|ENA first public:2024 03 15|Exposure time:24 hpf|External Id:SAMEA13395713|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:53:12Z|INSDC status:public|Submitter Id:S TOXR1832:S 24hpf DMSO UHEI|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1832:S 24hpf DMSO UHEI|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1832:S 24hpf DMSO UHEI e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136288 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_24hpf_DMSO_UHEI.fastq.gz fastq 73932600.0 1478652.0 S TOXR1832:S 24hpf DMSO UHEI r 0:50 A:15585622;C:19420814;G:18681864;T:20234365;N:9935 50       15585622 19420814 18681864 20234365 9935 ERX8812301 ERS10998366 ERA10091710 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Pharyngula Embryo Cell Line Cell Line
10634 10634 ERR9269901 ERX8812298 ERS10998363 ERP136288 PRJEB51641 CS2 UHEI DART 96 120h raw data EUT053 S-TOXR1832 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13395710 University of Heidelberg Compound:H2O|Concentration:nan nan|ENA first public:2024 03 15|Exposure time:24 hpf|External Id:SAMEA13395710|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:53:11Z|INSDC status:public|Submitter Id:S TOXR1832:S 24hpf NK UHEI|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1832:S 24hpf NK UHEI|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1832:S 24hpf NK UHEI e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136288 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_24hpf_NK_UHEI.fastq.gz fastq 67979350.0 1359587.0 S TOXR1832:S 24hpf NK UHEI r 0:50 A:14147053;C:17803617;G:16985508;T:19033861;N:9311 50       14147053 17803617 16985508 19033861 9311 ERX8812298 ERS10998363 ERA10091710 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Pharyngula Embryo Cell Line Cell Line
10639 10639 ERR9269916 ERX8812313 ERS10998379 ERP136288 PRJEB51641 CS2 UHEI DART 96 120h raw data EUT053 S-TOXR1832 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       SAMEA13395725 University of Heidelberg Compound:VPA|Concentration:10.0 µM|ENA first public:2024 03 15|Exposure time:24 hpf|External Id:SAMEA13395725|Gender:null|INSDC center name:University of Heidelberg|INSDC last update:2022 03 15T10:53:12Z|INSDC status:public|Submitter Id:S TOXR1832:S 24hpf VPA10 UHEI|Treatment:repeated dose|Treatment scheme:daily|broker name:EU ToxRisk DCC|cell line:null|common name:zebrafish|sample name:S TOXR1832:S 24hpf VPA10 UHEI|scientific name:Danio rerio                 Illumina HiSeq 2500 sequencing S TOXR1832:S 24hpf VPA10 UHEI e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136288 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_24hpf_VPA10_UHEI.fastq.gz fastq 81076400.0 1621528.0 S TOXR1832:S 24hpf VPA10 UHEI r 0:50 A:16717338;C:20603555;G:18834227;T:24910578;N:10702 50       16717338 20603555 18834227 24910578 10702 ERX8812313 ERS10998379 ERA10091710 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Pharyngula Embryo Cell Line Cell Line
10647 10647 ERR9269910 ERX8812307 ERS10998373 ERP136288 PRJEB51641 CS2 UHEI DART 96 120h raw data EUT053 S-TOXR1832 Transcriptome Analysis   ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15       S TOXR1832:S 24hpf VPA100 UHEI   Treatment:repeated dose|Treatment scheme:daily|organism:Danio rerio|cell line:null|Compound:VPA|scientific name:Danio rerio|Concentration:100.0 µM|Exposure time:24 hpf|Gender:null|common name:zebrafish|ENA LAST UPDATE:2022 03 15                 Illumina HiSeq 2500 sequencing S TOXR1832:S 24hpf VPA100 UHEI e   TempO Seq library     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA Illumina HiSeq 2500   ERP136288 Illumina HiSeq 2500 sequencing ENA FIRST PUBLIC:2024 03 15|ENA LAST UPDATE:2024 03 15 S_24hpf_VPA100_UHEI.fastq.gz fastq 91586100.0 1831722.0 S TOXR1832:S 24hpf VPA100 UHEI r 0:50 A:19049656;C:23685082;G:22248578;T:26590272;N:12512 50       19049656 23685082 22248578 26590272 12512 ERX8812307 ERS10998373 ERA10091710 EMBL EBI EMBL EBI                       B   usable mapping rate illumina hiseq_era unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2022-03-15 Pharyngula Embryo Cell Line Cell Line
15070 15070 ERR12476466 ERX11852287 ERS17743541 ERP156655 PRJEB71869 Effects of paternal starvation in the offspring development of zebrafish 33b9d14c-4211-4249-aede-f0f021d197e6 Other Dietary restriction in the form of fasting is a putative key to a healthier and longer life but these benefits may come at a trade off with reproductive fitness and may affect the following generations. The potential inter and transgenerational effects of long term fasting and starvation are particularly poorly understood in vertebrates when they originate from the paternal line. We utilised the externally fertilising zebrafish amenable to a split egg clutch design to explore the male specific effects of fasting/starvation on fertility and fitness of offspring independently of maternal contribution. Eighteen days of fasting resulted in reduced fertility in exposed males. While average offspring survival was not affected we detected increased larval growth rate in F1 offspring from starved males and more malformed embryos at 24 hpf in F2 offspring produced by F1 offspring from starved males. Comparing the transcriptomes of F1 embryos sired by starved and fed fathers revealed robust and reproducible increased expression of muscle composition genes but lower expression of lipid metabolism and lysosome genes in embryos from starved fathers. A large proportion of these genes showed enrichment in the yolk syncytial layer suggesting gene regulatory responses associated with metabolism of nutrients through paternal effects on extra embryonic tissues which are loaded with maternal factors. We compared the embryo transcriptomes to published adult transcriptome datasets and found comparable repressive effects of starvation on metabolism associated genes. These similarities suggest a physiologically relevant directed and potentially adaptive response transmitted by the father independently from the offspring's nutritional state which was defined by the mother. ENA FIRST PUBLIC:2024 01 15|ENA LAST UPDATE:2024 01 15   24 hpf embryo collected at 1219 from a cross with a starved father 1219 Starved 1219S   organism:Danio rerio|collection date:2018 02 02|scientific name:Danio rerio|common name:zebrafish|geographic location country and/or sea:Sweden                 NextSeq 500 sequencing ena EXPERIMENT TAB 15 01 2024 21:42:36:193 277029 1219S 1     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA NextSeq 500   ERP156655 NextSeq 500 sequencing ENA FIRST PUBLIC:2024 01 22|ENA LAST UPDATE:2024 01 22 1219S_S12_L003_R1_001.fastq.gz fastq 97592891.0 1298822.0 ena RUN TAB 15 01 2024 21:42:36:193 277030 0:75.14 A:34708924;C:19086989;G:21325291;T:22442527;N:29160 75       34708924 19086989 21325291 22442527 29160 ERX11852287 ERS17743541 ERA27788968 University of Birmingham|European Nucleotide Archive University of Birmingham                       B   usable mapping rate illumina nextseq unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2024-01-15 Pharyngula Embryo Embryo Imprecise All anatomical structures
15071 15071 ERR12476447 ERX11852268 ERS17743536 ERP156655 PRJEB71869 Effects of paternal starvation in the offspring development of zebrafish 33b9d14c-4211-4249-aede-f0f021d197e6 Other Dietary restriction in the form of fasting is a putative key to a healthier and longer life but these benefits may come at a trade off with reproductive fitness and may affect the following generations. The potential inter and transgenerational effects of long term fasting and starvation are particularly poorly understood in vertebrates when they originate from the paternal line. We utilised the externally fertilising zebrafish amenable to a split egg clutch design to explore the male specific effects of fasting/starvation on fertility and fitness of offspring independently of maternal contribution. Eighteen days of fasting resulted in reduced fertility in exposed males. While average offspring survival was not affected we detected increased larval growth rate in F1 offspring from starved males and more malformed embryos at 24 hpf in F2 offspring produced by F1 offspring from starved males. Comparing the transcriptomes of F1 embryos sired by starved and fed fathers revealed robust and reproducible increased expression of muscle composition genes but lower expression of lipid metabolism and lysosome genes in embryos from starved fathers. A large proportion of these genes showed enrichment in the yolk syncytial layer suggesting gene regulatory responses associated with metabolism of nutrients through paternal effects on extra embryonic tissues which are loaded with maternal factors. We compared the embryo transcriptomes to published adult transcriptome datasets and found comparable repressive effects of starvation on metabolism associated genes. These similarities suggest a physiologically relevant directed and potentially adaptive response transmitted by the father independently from the offspring's nutritional state which was defined by the mother. ENA FIRST PUBLIC:2024 01 15|ENA LAST UPDATE:2024 01 15   24 hpf embryo collected at 1203 from a cross with a fed father 1203 Fed 1203F   organism:Danio rerio|collection date:2018 02 02|scientific name:Danio rerio|common name:zebrafish|geographic location country and/or sea:Sweden                 NextSeq 500 sequencing ena EXPERIMENT TAB 15 01 2024 21:42:36:179 276991 1203F 1     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA NextSeq 500   ERP156655 NextSeq 500 sequencing ENA FIRST PUBLIC:2024 01 22|ENA LAST UPDATE:2024 01 22 1203F_S5_L004_R1_001.fastq.gz fastq 92134036.0 1226106.0 ena RUN TAB 15 01 2024 21:42:36:179 276992 0:75.14 A:33032252;C:17497320;G:19713134;T:21873197;N:18133 75       33032252 17497320 19713134 21873197 18133 ERX11852268 ERS17743536 ERA27788968 University of Birmingham|European Nucleotide Archive University of Birmingham                       B   usable mapping rate illumina nextseq unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2024-01-15 Pharyngula Embryo Embryo Imprecise All anatomical structures
15072 15072 ERR12476437 ERX11852258 ERS17743534 ERP156655 PRJEB71869 Effects of paternal starvation in the offspring development of zebrafish 33b9d14c-4211-4249-aede-f0f021d197e6 Other Dietary restriction in the form of fasting is a putative key to a healthier and longer life but these benefits may come at a trade off with reproductive fitness and may affect the following generations. The potential inter and transgenerational effects of long term fasting and starvation are particularly poorly understood in vertebrates when they originate from the paternal line. We utilised the externally fertilising zebrafish amenable to a split egg clutch design to explore the male specific effects of fasting/starvation on fertility and fitness of offspring independently of maternal contribution. Eighteen days of fasting resulted in reduced fertility in exposed males. While average offspring survival was not affected we detected increased larval growth rate in F1 offspring from starved males and more malformed embryos at 24 hpf in F2 offspring produced by F1 offspring from starved males. Comparing the transcriptomes of F1 embryos sired by starved and fed fathers revealed robust and reproducible increased expression of muscle composition genes but lower expression of lipid metabolism and lysosome genes in embryos from starved fathers. A large proportion of these genes showed enrichment in the yolk syncytial layer suggesting gene regulatory responses associated with metabolism of nutrients through paternal effects on extra embryonic tissues which are loaded with maternal factors. We compared the embryo transcriptomes to published adult transcriptome datasets and found comparable repressive effects of starvation on metabolism associated genes. These similarities suggest a physiologically relevant directed and potentially adaptive response transmitted by the father independently from the offspring's nutritional state which was defined by the mother. ENA FIRST PUBLIC:2024 01 15|ENA LAST UPDATE:2024 01 15   24 hpf embryo collected at 1118 from a cross with a fed father 1118 Fed 1118F   organism:Danio rerio|collection date:2018 02 02|scientific name:Danio rerio|common name:zebrafish|geographic location country and/or sea:Sweden                 NextSeq 500 sequencing ena EXPERIMENT TAB 15 01 2024 21:42:36:172 276971 1118F 1     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA NextSeq 500   ERP156655 NextSeq 500 sequencing ENA FIRST PUBLIC:2024 01 22|ENA LAST UPDATE:2024 01 22 1118F_S9_L002_R1_001.fastq.gz fastq 98606332.0 1311676.0 ena RUN TAB 15 01 2024 21:42:36:172 276972 0:75.18 A:35097475;C:19013865;G:21072825;T:23400976;N:21191 75       35097475 19013865 21072825 23400976 21191 ERX11852258 ERS17743534 ERA27788968 University of Birmingham|European Nucleotide Archive University of Birmingham                       B   usable mapping rate illumina nextseq unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2024-01-15 Pharyngula Embryo Embryo Imprecise All anatomical structures
15073 15073 ERR12476468 ERX11852289 ERS17743542 ERP156655 PRJEB71869 Effects of paternal starvation in the offspring development of zebrafish 33b9d14c-4211-4249-aede-f0f021d197e6 Other Dietary restriction in the form of fasting is a putative key to a healthier and longer life but these benefits may come at a trade off with reproductive fitness and may affect the following generations. The potential inter and transgenerational effects of long term fasting and starvation are particularly poorly understood in vertebrates when they originate from the paternal line. We utilised the externally fertilising zebrafish amenable to a split egg clutch design to explore the male specific effects of fasting/starvation on fertility and fitness of offspring independently of maternal contribution. Eighteen days of fasting resulted in reduced fertility in exposed males. While average offspring survival was not affected we detected increased larval growth rate in F1 offspring from starved males and more malformed embryos at 24 hpf in F2 offspring produced by F1 offspring from starved males. Comparing the transcriptomes of F1 embryos sired by starved and fed fathers revealed robust and reproducible increased expression of muscle composition genes but lower expression of lipid metabolism and lysosome genes in embryos from starved fathers. A large proportion of these genes showed enrichment in the yolk syncytial layer suggesting gene regulatory responses associated with metabolism of nutrients through paternal effects on extra embryonic tissues which are loaded with maternal factors. We compared the embryo transcriptomes to published adult transcriptome datasets and found comparable repressive effects of starvation on metabolism associated genes. These similarities suggest a physiologically relevant directed and potentially adaptive response transmitted by the father independently from the offspring's nutritional state which was defined by the mother. ENA FIRST PUBLIC:2024 01 15|ENA LAST UPDATE:2024 01 15   24 hpf embryo collected at 1242 from a cross with a fed father 1242D Fed 1242FD   organism:Danio rerio|collection date:2018 02 02|scientific name:Danio rerio|common name:zebrafish|geographic location country and/or sea:Sweden                 NextSeq 500 sequencing ena EXPERIMENT TAB 15 01 2024 21:42:36:194 277033 1242FD 1     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA NextSeq 500   ERP156655 NextSeq 500 sequencing ENA FIRST PUBLIC:2024 01 22|ENA LAST UPDATE:2024 01 22 1242FD_S13_L001_R1_001.fastq.gz fastq 89342488.0 1186885.0 ena RUN TAB 15 01 2024 21:42:36:194 277034 0:75.27 A:31174342;C:17129181;G:18704493;T:22323017;N:11455 75       31174342 17129181 18704493 22323017 11455 ERX11852289 ERS17743542 ERA27788968 University of Birmingham|European Nucleotide Archive University of Birmingham                       B   usable mapping rate illumina nextseq unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2024-01-15 Pharyngula Embryo Embryo Imprecise All anatomical structures
15074 15074 ERR12476438 ERX11852259 ERS17743534 ERP156655 PRJEB71869 Effects of paternal starvation in the offspring development of zebrafish 33b9d14c-4211-4249-aede-f0f021d197e6 Other Dietary restriction in the form of fasting is a putative key to a healthier and longer life but these benefits may come at a trade off with reproductive fitness and may affect the following generations. The potential inter and transgenerational effects of long term fasting and starvation are particularly poorly understood in vertebrates when they originate from the paternal line. We utilised the externally fertilising zebrafish amenable to a split egg clutch design to explore the male specific effects of fasting/starvation on fertility and fitness of offspring independently of maternal contribution. Eighteen days of fasting resulted in reduced fertility in exposed males. While average offspring survival was not affected we detected increased larval growth rate in F1 offspring from starved males and more malformed embryos at 24 hpf in F2 offspring produced by F1 offspring from starved males. Comparing the transcriptomes of F1 embryos sired by starved and fed fathers revealed robust and reproducible increased expression of muscle composition genes but lower expression of lipid metabolism and lysosome genes in embryos from starved fathers. A large proportion of these genes showed enrichment in the yolk syncytial layer suggesting gene regulatory responses associated with metabolism of nutrients through paternal effects on extra embryonic tissues which are loaded with maternal factors. We compared the embryo transcriptomes to published adult transcriptome datasets and found comparable repressive effects of starvation on metabolism associated genes. These similarities suggest a physiologically relevant directed and potentially adaptive response transmitted by the father independently from the offspring's nutritional state which was defined by the mother. ENA FIRST PUBLIC:2024 01 15|ENA LAST UPDATE:2024 01 15   24 hpf embryo collected at 1118 from a cross with a fed father 1118 Fed 1118F   organism:Danio rerio|collection date:2018 02 02|scientific name:Danio rerio|common name:zebrafish|geographic location country and/or sea:Sweden                 NextSeq 500 sequencing ena EXPERIMENT TAB 15 01 2024 21:42:36:173 276973 1118F 1     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA NextSeq 500   ERP156655 NextSeq 500 sequencing ENA FIRST PUBLIC:2024 01 22|ENA LAST UPDATE:2024 01 22 1118F_S9_L003_R1_001.fastq.gz fastq 98443412.0 1309747.0 ena RUN TAB 15 01 2024 21:42:36:173 276974 0:75.16 A:35171021;C:18991511;G:20974950;T:23280285;N:25645 75       35171021 18991511 20974950 23280285 25645 ERX11852259 ERS17743534 ERA27788968 University of Birmingham|European Nucleotide Archive University of Birmingham                       B   usable mapping rate illumina nextseq unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2024-01-15 Pharyngula Embryo Embryo Imprecise All anatomical structures
15075 15075 ERR12476442 ERX11852263 ERS17743535 ERP156655 PRJEB71869 Effects of paternal starvation in the offspring development of zebrafish 33b9d14c-4211-4249-aede-f0f021d197e6 Other Dietary restriction in the form of fasting is a putative key to a healthier and longer life but these benefits may come at a trade off with reproductive fitness and may affect the following generations. The potential inter and transgenerational effects of long term fasting and starvation are particularly poorly understood in vertebrates when they originate from the paternal line. We utilised the externally fertilising zebrafish amenable to a split egg clutch design to explore the male specific effects of fasting/starvation on fertility and fitness of offspring independently of maternal contribution. Eighteen days of fasting resulted in reduced fertility in exposed males. While average offspring survival was not affected we detected increased larval growth rate in F1 offspring from starved males and more malformed embryos at 24 hpf in F2 offspring produced by F1 offspring from starved males. Comparing the transcriptomes of F1 embryos sired by starved and fed fathers revealed robust and reproducible increased expression of muscle composition genes but lower expression of lipid metabolism and lysosome genes in embryos from starved fathers. A large proportion of these genes showed enrichment in the yolk syncytial layer suggesting gene regulatory responses associated with metabolism of nutrients through paternal effects on extra embryonic tissues which are loaded with maternal factors. We compared the embryo transcriptomes to published adult transcriptome datasets and found comparable repressive effects of starvation on metabolism associated genes. These similarities suggest a physiologically relevant directed and potentially adaptive response transmitted by the father independently from the offspring's nutritional state which was defined by the mother. ENA FIRST PUBLIC:2024 01 15|ENA LAST UPDATE:2024 01 15   24 hpf embryo collected at 1118 from a cross with a starved father 1118 Starved 1118S   organism:Danio rerio|collection date:2018 02 02|scientific name:Danio rerio|common name:zebrafish|geographic location country and/or sea:Sweden                 NextSeq 500 sequencing ena EXPERIMENT TAB 15 01 2024 21:42:36:176 276981 1118S 1     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA NextSeq 500   ERP156655 NextSeq 500 sequencing ENA FIRST PUBLIC:2024 01 22|ENA LAST UPDATE:2024 01 22 1118S_S10_L003_R1_001.fastq.gz fastq 98865255.0 1315357.0 ena RUN TAB 15 01 2024 21:42:36:176 276982 0:75.16 A:34981471;C:19126822;G:21304286;T:23427226;N:25450 75       34981471 19126822 21304286 23427226 25450 ERX11852263 ERS17743535 ERA27788968 University of Birmingham|European Nucleotide Archive University of Birmingham                       B   usable mapping rate illumina nextseq unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2024-01-15 Pharyngula Embryo Embryo Imprecise All anatomical structures
15076 15076 ERR12476467 ERX11852288 ERS17743541 ERP156655 PRJEB71869 Effects of paternal starvation in the offspring development of zebrafish 33b9d14c-4211-4249-aede-f0f021d197e6 Other Dietary restriction in the form of fasting is a putative key to a healthier and longer life but these benefits may come at a trade off with reproductive fitness and may affect the following generations. The potential inter and transgenerational effects of long term fasting and starvation are particularly poorly understood in vertebrates when they originate from the paternal line. We utilised the externally fertilising zebrafish amenable to a split egg clutch design to explore the male specific effects of fasting/starvation on fertility and fitness of offspring independently of maternal contribution. Eighteen days of fasting resulted in reduced fertility in exposed males. While average offspring survival was not affected we detected increased larval growth rate in F1 offspring from starved males and more malformed embryos at 24 hpf in F2 offspring produced by F1 offspring from starved males. Comparing the transcriptomes of F1 embryos sired by starved and fed fathers revealed robust and reproducible increased expression of muscle composition genes but lower expression of lipid metabolism and lysosome genes in embryos from starved fathers. A large proportion of these genes showed enrichment in the yolk syncytial layer suggesting gene regulatory responses associated with metabolism of nutrients through paternal effects on extra embryonic tissues which are loaded with maternal factors. We compared the embryo transcriptomes to published adult transcriptome datasets and found comparable repressive effects of starvation on metabolism associated genes. These similarities suggest a physiologically relevant directed and potentially adaptive response transmitted by the father independently from the offspring's nutritional state which was defined by the mother. ENA FIRST PUBLIC:2024 01 15|ENA LAST UPDATE:2024 01 15   24 hpf embryo collected at 1219 from a cross with a starved father 1219 Starved 1219S   organism:Danio rerio|collection date:2018 02 02|scientific name:Danio rerio|common name:zebrafish|geographic location country and/or sea:Sweden                 NextSeq 500 sequencing ena EXPERIMENT TAB 15 01 2024 21:42:36:193 277031 1219S 1     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA NextSeq 500   ERP156655 NextSeq 500 sequencing ENA FIRST PUBLIC:2024 01 22|ENA LAST UPDATE:2024 01 22 1219S_S12_L004_R1_001.fastq.gz fastq 88230016.0 1174256.0 ena RUN TAB 15 01 2024 21:42:36:194 277032 0:75.14 A:31409300;C:17203675;G:19284478;T:20309174;N:23389 75       31409300 17203675 19284478 20309174 23389 ERX11852288 ERS17743541 ERA27788968 University of Birmingham|European Nucleotide Archive University of Birmingham                       B   usable mapping rate illumina nextseq unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2024-01-15 Pharyngula Embryo Embryo Imprecise All anatomical structures
15077 15077 ERR12476470 ERX11852291 ERS17743542 ERP156655 PRJEB71869 Effects of paternal starvation in the offspring development of zebrafish 33b9d14c-4211-4249-aede-f0f021d197e6 Other Dietary restriction in the form of fasting is a putative key to a healthier and longer life but these benefits may come at a trade off with reproductive fitness and may affect the following generations. The potential inter and transgenerational effects of long term fasting and starvation are particularly poorly understood in vertebrates when they originate from the paternal line. We utilised the externally fertilising zebrafish amenable to a split egg clutch design to explore the male specific effects of fasting/starvation on fertility and fitness of offspring independently of maternal contribution. Eighteen days of fasting resulted in reduced fertility in exposed males. While average offspring survival was not affected we detected increased larval growth rate in F1 offspring from starved males and more malformed embryos at 24 hpf in F2 offspring produced by F1 offspring from starved males. Comparing the transcriptomes of F1 embryos sired by starved and fed fathers revealed robust and reproducible increased expression of muscle composition genes but lower expression of lipid metabolism and lysosome genes in embryos from starved fathers. A large proportion of these genes showed enrichment in the yolk syncytial layer suggesting gene regulatory responses associated with metabolism of nutrients through paternal effects on extra embryonic tissues which are loaded with maternal factors. We compared the embryo transcriptomes to published adult transcriptome datasets and found comparable repressive effects of starvation on metabolism associated genes. These similarities suggest a physiologically relevant directed and potentially adaptive response transmitted by the father independently from the offspring's nutritional state which was defined by the mother. ENA FIRST PUBLIC:2024 01 15|ENA LAST UPDATE:2024 01 15   24 hpf embryo collected at 1242 from a cross with a fed father 1242D Fed 1242FD   organism:Danio rerio|collection date:2018 02 02|scientific name:Danio rerio|common name:zebrafish|geographic location country and/or sea:Sweden                 NextSeq 500 sequencing ena EXPERIMENT TAB 15 01 2024 21:42:36:195 277037 1242FD 1     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA NextSeq 500   ERP156655 NextSeq 500 sequencing ENA FIRST PUBLIC:2024 01 22|ENA LAST UPDATE:2024 01 22 1242FD_S13_L003_R1_001.fastq.gz fastq 93375090.0 1240903.0 ena RUN TAB 15 01 2024 21:42:36:196 277038 0:75.25 A:32510114;C:17950920;G:19575617;T:23324862;N:13577 75       32510114 17950920 19575617 23324862 13577 ERX11852291 ERS17743542 ERA27788968 University of Birmingham|European Nucleotide Archive University of Birmingham                       B   usable mapping rate illumina nextseq unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2024-01-15 Pharyngula Embryo Embryo Imprecise All anatomical structures
15078 15078 ERR12476436 ERX11852257 ERS17743534 ERP156655 PRJEB71869 Effects of paternal starvation in the offspring development of zebrafish 33b9d14c-4211-4249-aede-f0f021d197e6 Other Dietary restriction in the form of fasting is a putative key to a healthier and longer life but these benefits may come at a trade off with reproductive fitness and may affect the following generations. The potential inter and transgenerational effects of long term fasting and starvation are particularly poorly understood in vertebrates when they originate from the paternal line. We utilised the externally fertilising zebrafish amenable to a split egg clutch design to explore the male specific effects of fasting/starvation on fertility and fitness of offspring independently of maternal contribution. Eighteen days of fasting resulted in reduced fertility in exposed males. While average offspring survival was not affected we detected increased larval growth rate in F1 offspring from starved males and more malformed embryos at 24 hpf in F2 offspring produced by F1 offspring from starved males. Comparing the transcriptomes of F1 embryos sired by starved and fed fathers revealed robust and reproducible increased expression of muscle composition genes but lower expression of lipid metabolism and lysosome genes in embryos from starved fathers. A large proportion of these genes showed enrichment in the yolk syncytial layer suggesting gene regulatory responses associated with metabolism of nutrients through paternal effects on extra embryonic tissues which are loaded with maternal factors. We compared the embryo transcriptomes to published adult transcriptome datasets and found comparable repressive effects of starvation on metabolism associated genes. These similarities suggest a physiologically relevant directed and potentially adaptive response transmitted by the father independently from the offspring's nutritional state which was defined by the mother. ENA FIRST PUBLIC:2024 01 15|ENA LAST UPDATE:2024 01 15   24 hpf embryo collected at 1118 from a cross with a fed father 1118 Fed 1118F   organism:Danio rerio|collection date:2018 02 02|scientific name:Danio rerio|common name:zebrafish|geographic location country and/or sea:Sweden                 NextSeq 500 sequencing ena EXPERIMENT TAB 15 01 2024 21:42:36:171 276969 1118F 1     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA NextSeq 500   ERP156655 NextSeq 500 sequencing ENA FIRST PUBLIC:2024 01 22|ENA LAST UPDATE:2024 01 22 1118F_S9_L001_R1_001.fastq.gz fastq 94258948.0 1253428.0 ena RUN TAB 15 01 2024 21:42:36:171 276970 0:75.20 A:33713614;C:18157124;G:20086205;T:22277451;N:24554 75       33713614 18157124 20086205 22277451 24554 ERX11852257 ERS17743534 ERA27788968 University of Birmingham|European Nucleotide Archive University of Birmingham                       B   usable mapping rate illumina nextseq unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2024-01-15 Pharyngula Embryo Embryo Imprecise All anatomical structures
15079 15079 ERR12476452 ERX11852273 ERS17743538 ERP156655 PRJEB71869 Effects of paternal starvation in the offspring development of zebrafish 33b9d14c-4211-4249-aede-f0f021d197e6 Other Dietary restriction in the form of fasting is a putative key to a healthier and longer life but these benefits may come at a trade off with reproductive fitness and may affect the following generations. The potential inter and transgenerational effects of long term fasting and starvation are particularly poorly understood in vertebrates when they originate from the paternal line. We utilised the externally fertilising zebrafish amenable to a split egg clutch design to explore the male specific effects of fasting/starvation on fertility and fitness of offspring independently of maternal contribution. Eighteen days of fasting resulted in reduced fertility in exposed males. While average offspring survival was not affected we detected increased larval growth rate in F1 offspring from starved males and more malformed embryos at 24 hpf in F2 offspring produced by F1 offspring from starved males. Comparing the transcriptomes of F1 embryos sired by starved and fed fathers revealed robust and reproducible increased expression of muscle composition genes but lower expression of lipid metabolism and lysosome genes in embryos from starved fathers. A large proportion of these genes showed enrichment in the yolk syncytial layer suggesting gene regulatory responses associated with metabolism of nutrients through paternal effects on extra embryonic tissues which are loaded with maternal factors. We compared the embryo transcriptomes to published adult transcriptome datasets and found comparable repressive effects of starvation on metabolism associated genes. These similarities suggest a physiologically relevant directed and potentially adaptive response transmitted by the father independently from the offspring's nutritional state which was defined by the mother. ENA FIRST PUBLIC:2024 01 15|ENA LAST UPDATE:2024 01 15   24 hpf embryo collected at 1210 from a cross with a fed father 1210 Fed 1210F   organism:Danio rerio|collection date:2018 02 02|scientific name:Danio rerio|common name:zebrafish|geographic location country and/or sea:Sweden                 NextSeq 500 sequencing ena EXPERIMENT TAB 15 01 2024 21:42:36:183 277001 1210F 1     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA NextSeq 500   ERP156655 NextSeq 500 sequencing ENA FIRST PUBLIC:2024 01 22|ENA LAST UPDATE:2024 01 22 1210F_S7_L001_R1_001.fastq.gz fastq 96414625.0 1283672.0 ena RUN TAB 15 01 2024 21:42:36:183 277002 0:75.11 A:35605799;C:18366729;G:20807908;T:21594044;N:40145 75       35605799 18366729 20807908 21594044 40145 ERX11852273 ERS17743538 ERA27788968 University of Birmingham|European Nucleotide Archive University of Birmingham                       B   usable mapping rate illumina nextseq unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2024-01-15 Pharyngula Embryo Embryo Imprecise All anatomical structures
15080 15080 ERR12476475 ERX11852296 ERS17743544 ERP156655 PRJEB71869 Effects of paternal starvation in the offspring development of zebrafish 33b9d14c-4211-4249-aede-f0f021d197e6 Other Dietary restriction in the form of fasting is a putative key to a healthier and longer life but these benefits may come at a trade off with reproductive fitness and may affect the following generations. The potential inter and transgenerational effects of long term fasting and starvation are particularly poorly understood in vertebrates when they originate from the paternal line. We utilised the externally fertilising zebrafish amenable to a split egg clutch design to explore the male specific effects of fasting/starvation on fertility and fitness of offspring independently of maternal contribution. Eighteen days of fasting resulted in reduced fertility in exposed males. While average offspring survival was not affected we detected increased larval growth rate in F1 offspring from starved males and more malformed embryos at 24 hpf in F2 offspring produced by F1 offspring from starved males. Comparing the transcriptomes of F1 embryos sired by starved and fed fathers revealed robust and reproducible increased expression of muscle composition genes but lower expression of lipid metabolism and lysosome genes in embryos from starved fathers. A large proportion of these genes showed enrichment in the yolk syncytial layer suggesting gene regulatory responses associated with metabolism of nutrients through paternal effects on extra embryonic tissues which are loaded with maternal factors. We compared the embryo transcriptomes to published adult transcriptome datasets and found comparable repressive effects of starvation on metabolism associated genes. These similarities suggest a physiologically relevant directed and potentially adaptive response transmitted by the father independently from the offspring's nutritional state which was defined by the mother. ENA FIRST PUBLIC:2024 01 15|ENA LAST UPDATE:2024 01 15   24 hpf embryo collected at 1242 from a cross with a fed father 1242E Fed 1242FE   organism:Danio rerio|collection date:2018 02 02|scientific name:Danio rerio|common name:zebrafish|geographic location country and/or sea:Sweden                 NextSeq 500 sequencing ena EXPERIMENT TAB 15 01 2024 21:42:36:199 277047 1242FE 1     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA NextSeq 500   ERP156655 NextSeq 500 sequencing ENA FIRST PUBLIC:2024 01 22|ENA LAST UPDATE:2024 01 22 1242FE_S15_L004_R1_001.fastq.gz fastq 99581325.0 1325945.0 ena RUN TAB 15 01 2024 21:42:36:199 277048 0:75.10 A:36802442;C:18868511;G:20958294;T:22926783;N:25295 75       36802442 18868511 20958294 22926783 25295 ERX11852296 ERS17743544 ERA27788968 University of Birmingham|European Nucleotide Archive University of Birmingham                       B   usable mapping rate illumina nextseq unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2024-01-15 Pharyngula Embryo Embryo Imprecise All anatomical structures
15081 15081 ERR12476478 ERX11852299 ERS17743543 ERP156655 PRJEB71869 Effects of paternal starvation in the offspring development of zebrafish 33b9d14c-4211-4249-aede-f0f021d197e6 Other Dietary restriction in the form of fasting is a putative key to a healthier and longer life but these benefits may come at a trade off with reproductive fitness and may affect the following generations. The potential inter and transgenerational effects of long term fasting and starvation are particularly poorly understood in vertebrates when they originate from the paternal line. We utilised the externally fertilising zebrafish amenable to a split egg clutch design to explore the male specific effects of fasting/starvation on fertility and fitness of offspring independently of maternal contribution. Eighteen days of fasting resulted in reduced fertility in exposed males. While average offspring survival was not affected we detected increased larval growth rate in F1 offspring from starved males and more malformed embryos at 24 hpf in F2 offspring produced by F1 offspring from starved males. Comparing the transcriptomes of F1 embryos sired by starved and fed fathers revealed robust and reproducible increased expression of muscle composition genes but lower expression of lipid metabolism and lysosome genes in embryos from starved fathers. A large proportion of these genes showed enrichment in the yolk syncytial layer suggesting gene regulatory responses associated with metabolism of nutrients through paternal effects on extra embryonic tissues which are loaded with maternal factors. We compared the embryo transcriptomes to published adult transcriptome datasets and found comparable repressive effects of starvation on metabolism associated genes. These similarities suggest a physiologically relevant directed and potentially adaptive response transmitted by the father independently from the offspring's nutritional state which was defined by the mother. ENA FIRST PUBLIC:2024 01 15|ENA LAST UPDATE:2024 01 15   24 hpf embryo collected at 1242 from a cross with a starved father 1242D Starved 1242SD   organism:Danio rerio|collection date:2018 02 02|scientific name:Danio rerio|common name:zebrafish|geographic location country and/or sea:Sweden                 NextSeq 500 sequencing ena EXPERIMENT TAB 15 01 2024 21:42:36:201 277053 1242SD 1     RNA-Seq TRANSCRIPTOMIC size fractionation SINGLE ILLUMINA NextSeq 500   ERP156655 NextSeq 500 sequencing ENA FIRST PUBLIC:2024 01 22|ENA LAST UPDATE:2024 01 22 1242SD_S14_L003_R1_001.fastq.gz fastq 95457398.0 1268859.0 ena RUN TAB 15 01 2024 21:42:36:202 277054 0:75.23 A:33339157;C:18598733;G:20350072;T:23148005;N:21431 75       33339157 18598733 20350072 23148005 21431 ERX11852299 ERS17743543 ERA27788968 University of Birmingham|European Nucleotide Archive University of Birmingham                       B   usable mapping rate illumina nextseq unknown size_fractionation unknown bulk unknown unknown   United Kingdom 2024-01-15 Pharyngula Embryo Embryo Imprecise All anatomical structures
15082 15082 ERR12476473 ERX11852294 ERS17743544 ERP156655 PRJEB71869 Effects of paternal starvation in the offspring development of zebrafish 33b9d14c-4211-4249-aede-f0f021d197e6 Other Dietary restriction in the form of fasting is a putative key to a healthier and longer life but these benefits may come at a trade off with reproductive fitness and may affect the following generations. The potential inter and transgenerational effects of long term fasting and starvation are particularly poorly understood in vertebrates when they originate from the paternal line. We utilised the externally fertilising zebrafish amenable to a split egg clutch design to explore the male specific effects of fasting/starvation on fertility and fitness of offspring independently of maternal contribution. Eighteen days of fasting resulted in reduced fertility in exposed males. While average offspring survival was not affected we detected increased larval growth rate in F1 offspring from starved males and more malformed embryos at 24 hpf in F2 offspring produced by F1 offspring from starved males. Comparing the transcriptomes of F1 embryos sired by starved and fed fathers revealed robust and reproducible increased expression of muscle composition genes but lower expression of lipid metabolism and lysosome genes in embryos from starved fathers. A large proportion of these genes showed enrichment in the yolk syncytial layer suggesting gene regulatory responses associated with metabolism of nutrients through paternal effects on extra embryonic tissues which are loaded with maternal factors. We compared the embryo transcriptomes to published adult transcriptome datasets and found comparable repressive effects of starvation on metabolism associated genes. These similarities suggest a physiologically relevant directed and potentially adaptive response transmitted by the father independently from the offspring's nutritional state which was defined by the mother. ENA FIRST PUBLIC:2024 01 15|ENA LAST UPDATE:2024 01 15   24 hpf embryo collected at 1242 from a cross with a fed father 1242E Fed 1242FE   organism:Danio rerio|collection date:2018 02 02|scientific name:Danio rerio|common name:zebrafish|geographic location country and/or sea:Sweden                 NextSeq 500 sequencing ena EXPERIMENT TAB 15 01 2024 21:42:36:198 277043 1242FE 1     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA NextSeq 500   ERP156655 NextSeq 500 sequencing ENA FIRST PUBLIC:2024 01 22|ENA LAST UPDATE:2024 01 22 1242FE_S15_L002_R1_001.fastq.gz fastq 111191327.0 1479982.0 ena RUN TAB 15 01 2024 21:42:36:198 277044 0:75.13 A:40873794;C:21118758;G:23508088;T:25663516;N:27171 75       40873794 21118758 23508088 25663516 27171 ERX11852294 ERS17743544 ERA27788968 University of Birmingham|European Nucleotide Archive University of Birmingham                       B   usable mapping rate illumina nextseq unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2024-01-15 Pharyngula Embryo Embryo Imprecise All anatomical structures
15083 15083 ERR12476472 ERX11852293 ERS17743544 ERP156655 PRJEB71869 Effects of paternal starvation in the offspring development of zebrafish 33b9d14c-4211-4249-aede-f0f021d197e6 Other Dietary restriction in the form of fasting is a putative key to a healthier and longer life but these benefits may come at a trade off with reproductive fitness and may affect the following generations. The potential inter and transgenerational effects of long term fasting and starvation are particularly poorly understood in vertebrates when they originate from the paternal line. We utilised the externally fertilising zebrafish amenable to a split egg clutch design to explore the male specific effects of fasting/starvation on fertility and fitness of offspring independently of maternal contribution. Eighteen days of fasting resulted in reduced fertility in exposed males. While average offspring survival was not affected we detected increased larval growth rate in F1 offspring from starved males and more malformed embryos at 24 hpf in F2 offspring produced by F1 offspring from starved males. Comparing the transcriptomes of F1 embryos sired by starved and fed fathers revealed robust and reproducible increased expression of muscle composition genes but lower expression of lipid metabolism and lysosome genes in embryos from starved fathers. A large proportion of these genes showed enrichment in the yolk syncytial layer suggesting gene regulatory responses associated with metabolism of nutrients through paternal effects on extra embryonic tissues which are loaded with maternal factors. We compared the embryo transcriptomes to published adult transcriptome datasets and found comparable repressive effects of starvation on metabolism associated genes. These similarities suggest a physiologically relevant directed and potentially adaptive response transmitted by the father independently from the offspring's nutritional state which was defined by the mother. ENA FIRST PUBLIC:2024 01 15|ENA LAST UPDATE:2024 01 15   24 hpf embryo collected at 1242 from a cross with a fed father 1242E Fed 1242FE   organism:Danio rerio|collection date:2018 02 02|scientific name:Danio rerio|common name:zebrafish|geographic location country and/or sea:Sweden                 NextSeq 500 sequencing ena EXPERIMENT TAB 15 01 2024 21:42:36:197 277041 1242FE 1     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA NextSeq 500   ERP156655 NextSeq 500 sequencing ENA FIRST PUBLIC:2024 01 22|ENA LAST UPDATE:2024 01 22 1242FE_S15_L001_R1_001.fastq.gz fastq 105936834.0 1409443.0 ena RUN TAB 15 01 2024 21:42:36:197 277042 0:75.16 A:39084749;C:20103850;G:22336806;T:24384711;N:26718 75       39084749 20103850 22336806 24384711 26718 ERX11852293 ERS17743544 ERA27788968 University of Birmingham|European Nucleotide Archive University of Birmingham                       B   usable mapping rate illumina nextseq unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2024-01-15 Pharyngula Embryo Embryo Imprecise All anatomical structures
15084 15084 ERR12476455 ERX11852276 ERS17743538 ERP156655 PRJEB71869 Effects of paternal starvation in the offspring development of zebrafish 33b9d14c-4211-4249-aede-f0f021d197e6 Other Dietary restriction in the form of fasting is a putative key to a healthier and longer life but these benefits may come at a trade off with reproductive fitness and may affect the following generations. The potential inter and transgenerational effects of long term fasting and starvation are particularly poorly understood in vertebrates when they originate from the paternal line. We utilised the externally fertilising zebrafish amenable to a split egg clutch design to explore the male specific effects of fasting/starvation on fertility and fitness of offspring independently of maternal contribution. Eighteen days of fasting resulted in reduced fertility in exposed males. While average offspring survival was not affected we detected increased larval growth rate in F1 offspring from starved males and more malformed embryos at 24 hpf in F2 offspring produced by F1 offspring from starved males. Comparing the transcriptomes of F1 embryos sired by starved and fed fathers revealed robust and reproducible increased expression of muscle composition genes but lower expression of lipid metabolism and lysosome genes in embryos from starved fathers. A large proportion of these genes showed enrichment in the yolk syncytial layer suggesting gene regulatory responses associated with metabolism of nutrients through paternal effects on extra embryonic tissues which are loaded with maternal factors. We compared the embryo transcriptomes to published adult transcriptome datasets and found comparable repressive effects of starvation on metabolism associated genes. These similarities suggest a physiologically relevant directed and potentially adaptive response transmitted by the father independently from the offspring's nutritional state which was defined by the mother. ENA FIRST PUBLIC:2024 01 15|ENA LAST UPDATE:2024 01 15   24 hpf embryo collected at 1210 from a cross with a fed father 1210 Fed 1210F   organism:Danio rerio|collection date:2018 02 02|scientific name:Danio rerio|common name:zebrafish|geographic location country and/or sea:Sweden                 NextSeq 500 sequencing ena EXPERIMENT TAB 15 01 2024 21:42:36:185 277007 1210F 1     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA NextSeq 500   ERP156655 NextSeq 500 sequencing ENA FIRST PUBLIC:2024 01 22|ENA LAST UPDATE:2024 01 22 1210F_S7_L004_R1_001.fastq.gz fastq 91003627.0 1212716.0 ena RUN TAB 15 01 2024 21:42:36:185 277008 0:75.04 A:33677348;C:17299066;G:19585548;T:20403064;N:38601 75       33677348 17299066 19585548 20403064 38601 ERX11852276 ERS17743538 ERA27788968 University of Birmingham|European Nucleotide Archive University of Birmingham                       B   usable mapping rate illumina nextseq unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2024-01-15 Pharyngula Embryo Embryo Imprecise All anatomical structures
15085 15085 ERR12476450 ERX11852271 ERS17743537 ERP156655 PRJEB71869 Effects of paternal starvation in the offspring development of zebrafish 33b9d14c-4211-4249-aede-f0f021d197e6 Other Dietary restriction in the form of fasting is a putative key to a healthier and longer life but these benefits may come at a trade off with reproductive fitness and may affect the following generations. The potential inter and transgenerational effects of long term fasting and starvation are particularly poorly understood in vertebrates when they originate from the paternal line. We utilised the externally fertilising zebrafish amenable to a split egg clutch design to explore the male specific effects of fasting/starvation on fertility and fitness of offspring independently of maternal contribution. Eighteen days of fasting resulted in reduced fertility in exposed males. While average offspring survival was not affected we detected increased larval growth rate in F1 offspring from starved males and more malformed embryos at 24 hpf in F2 offspring produced by F1 offspring from starved males. Comparing the transcriptomes of F1 embryos sired by starved and fed fathers revealed robust and reproducible increased expression of muscle composition genes but lower expression of lipid metabolism and lysosome genes in embryos from starved fathers. A large proportion of these genes showed enrichment in the yolk syncytial layer suggesting gene regulatory responses associated with metabolism of nutrients through paternal effects on extra embryonic tissues which are loaded with maternal factors. We compared the embryo transcriptomes to published adult transcriptome datasets and found comparable repressive effects of starvation on metabolism associated genes. These similarities suggest a physiologically relevant directed and potentially adaptive response transmitted by the father independently from the offspring's nutritional state which was defined by the mother. ENA FIRST PUBLIC:2024 01 15|ENA LAST UPDATE:2024 01 15   24 hpf embryo collected at 1203 from a cross with a starved father 1203 Starved 1203S   organism:Danio rerio|collection date:2018 02 02|scientific name:Danio rerio|common name:zebrafish|geographic location country and/or sea:Sweden                 NextSeq 500 sequencing ena EXPERIMENT TAB 15 01 2024 21:42:36:181 276997 1203S 1     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA NextSeq 500   ERP156655 NextSeq 500 sequencing ENA FIRST PUBLIC:2024 01 22|ENA LAST UPDATE:2024 01 22 1203S_S6_L003_R1_001.fastq.gz fastq 93998632.0 1251284.0 ena RUN TAB 15 01 2024 21:42:36:182 276998 0:75.12 A:33959433;C:18143368;G:20041026;T:21824242;N:30563 75       33959433 18143368 20041026 21824242 30563 ERX11852271 ERS17743537 ERA27788968 University of Birmingham|European Nucleotide Archive University of Birmingham                       B   usable mapping rate illumina nextseq unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2024-01-15 Pharyngula Embryo Embryo Imprecise All anatomical structures
15086 15086 ERR12476446 ERX11852267 ERS17743536 ERP156655 PRJEB71869 Effects of paternal starvation in the offspring development of zebrafish 33b9d14c-4211-4249-aede-f0f021d197e6 Other Dietary restriction in the form of fasting is a putative key to a healthier and longer life but these benefits may come at a trade off with reproductive fitness and may affect the following generations. The potential inter and transgenerational effects of long term fasting and starvation are particularly poorly understood in vertebrates when they originate from the paternal line. We utilised the externally fertilising zebrafish amenable to a split egg clutch design to explore the male specific effects of fasting/starvation on fertility and fitness of offspring independently of maternal contribution. Eighteen days of fasting resulted in reduced fertility in exposed males. While average offspring survival was not affected we detected increased larval growth rate in F1 offspring from starved males and more malformed embryos at 24 hpf in F2 offspring produced by F1 offspring from starved males. Comparing the transcriptomes of F1 embryos sired by starved and fed fathers revealed robust and reproducible increased expression of muscle composition genes but lower expression of lipid metabolism and lysosome genes in embryos from starved fathers. A large proportion of these genes showed enrichment in the yolk syncytial layer suggesting gene regulatory responses associated with metabolism of nutrients through paternal effects on extra embryonic tissues which are loaded with maternal factors. We compared the embryo transcriptomes to published adult transcriptome datasets and found comparable repressive effects of starvation on metabolism associated genes. These similarities suggest a physiologically relevant directed and potentially adaptive response transmitted by the father independently from the offspring's nutritional state which was defined by the mother. ENA FIRST PUBLIC:2024 01 15|ENA LAST UPDATE:2024 01 15   24 hpf embryo collected at 1203 from a cross with a fed father 1203 Fed 1203F   organism:Danio rerio|collection date:2018 02 02|scientific name:Danio rerio|common name:zebrafish|geographic location country and/or sea:Sweden                 NextSeq 500 sequencing ena EXPERIMENT TAB 15 01 2024 21:42:36:178 276989 1203F 1     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA NextSeq 500   ERP156655 NextSeq 500 sequencing ENA FIRST PUBLIC:2024 01 22|ENA LAST UPDATE:2024 01 22 1203F_S5_L003_R1_001.fastq.gz fastq 102209965.0 1360067.0 ena RUN TAB 15 01 2024 21:42:36:179 276990 0:75.15 A:36554629;C:19466103;G:21890238;T:24276196;N:22799 75       36554629 19466103 21890238 24276196 22799 ERX11852267 ERS17743536 ERA27788968 University of Birmingham|European Nucleotide Archive University of Birmingham                       B   usable mapping rate illumina nextseq unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2024-01-15 Pharyngula Embryo Embryo Imprecise All anatomical structures
15087 15087 ERR12476458 ERX11852279 ERS17743539 ERP156655 PRJEB71869 Effects of paternal starvation in the offspring development of zebrafish 33b9d14c-4211-4249-aede-f0f021d197e6 Other Dietary restriction in the form of fasting is a putative key to a healthier and longer life but these benefits may come at a trade off with reproductive fitness and may affect the following generations. The potential inter and transgenerational effects of long term fasting and starvation are particularly poorly understood in vertebrates when they originate from the paternal line. We utilised the externally fertilising zebrafish amenable to a split egg clutch design to explore the male specific effects of fasting/starvation on fertility and fitness of offspring independently of maternal contribution. Eighteen days of fasting resulted in reduced fertility in exposed males. While average offspring survival was not affected we detected increased larval growth rate in F1 offspring from starved males and more malformed embryos at 24 hpf in F2 offspring produced by F1 offspring from starved males. Comparing the transcriptomes of F1 embryos sired by starved and fed fathers revealed robust and reproducible increased expression of muscle composition genes but lower expression of lipid metabolism and lysosome genes in embryos from starved fathers. A large proportion of these genes showed enrichment in the yolk syncytial layer suggesting gene regulatory responses associated with metabolism of nutrients through paternal effects on extra embryonic tissues which are loaded with maternal factors. We compared the embryo transcriptomes to published adult transcriptome datasets and found comparable repressive effects of starvation on metabolism associated genes. These similarities suggest a physiologically relevant directed and potentially adaptive response transmitted by the father independently from the offspring's nutritional state which was defined by the mother. ENA FIRST PUBLIC:2024 01 15|ENA LAST UPDATE:2024 01 15   24 hpf embryo collected at 1210 from a cross with a starved father 1210 Starved 1210S   organism:Danio rerio|collection date:2018 02 02|scientific name:Danio rerio|common name:zebrafish|geographic location country and/or sea:Sweden                 NextSeq 500 sequencing ena EXPERIMENT TAB 15 01 2024 21:42:36:187 277013 1210S 1     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA NextSeq 500   ERP156655 NextSeq 500 sequencing ENA FIRST PUBLIC:2024 01 22|ENA LAST UPDATE:2024 01 22 1210S_S8_L003_R1_001.fastq.gz fastq 109602092.0 1466517.0 ena RUN TAB 15 01 2024 21:42:36:187 277014 0:74.74 A:42677564;C:20838229;G:24413347;T:21498031;N:174921 74       42677564 20838229 24413347 21498031 174921 ERX11852279 ERS17743539 ERA27788968 University of Birmingham|European Nucleotide Archive University of Birmingham                       B   usable mapping rate illumina nextseq unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2024-01-15 Pharyngula Embryo Embryo Imprecise All anatomical structures
15088 15088 ERR12476453 ERX11852274 ERS17743538 ERP156655 PRJEB71869 Effects of paternal starvation in the offspring development of zebrafish 33b9d14c-4211-4249-aede-f0f021d197e6 Other Dietary restriction in the form of fasting is a putative key to a healthier and longer life but these benefits may come at a trade off with reproductive fitness and may affect the following generations. The potential inter and transgenerational effects of long term fasting and starvation are particularly poorly understood in vertebrates when they originate from the paternal line. We utilised the externally fertilising zebrafish amenable to a split egg clutch design to explore the male specific effects of fasting/starvation on fertility and fitness of offspring independently of maternal contribution. Eighteen days of fasting resulted in reduced fertility in exposed males. While average offspring survival was not affected we detected increased larval growth rate in F1 offspring from starved males and more malformed embryos at 24 hpf in F2 offspring produced by F1 offspring from starved males. Comparing the transcriptomes of F1 embryos sired by starved and fed fathers revealed robust and reproducible increased expression of muscle composition genes but lower expression of lipid metabolism and lysosome genes in embryos from starved fathers. A large proportion of these genes showed enrichment in the yolk syncytial layer suggesting gene regulatory responses associated with metabolism of nutrients through paternal effects on extra embryonic tissues which are loaded with maternal factors. We compared the embryo transcriptomes to published adult transcriptome datasets and found comparable repressive effects of starvation on metabolism associated genes. These similarities suggest a physiologically relevant directed and potentially adaptive response transmitted by the father independently from the offspring's nutritional state which was defined by the mother. ENA FIRST PUBLIC:2024 01 15|ENA LAST UPDATE:2024 01 15   24 hpf embryo collected at 1210 from a cross with a fed father 1210 Fed 1210F   organism:Danio rerio|collection date:2018 02 02|scientific name:Danio rerio|common name:zebrafish|geographic location country and/or sea:Sweden                 NextSeq 500 sequencing ena EXPERIMENT TAB 15 01 2024 21:42:36:183 277003 1210F 1     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA NextSeq 500   ERP156655 NextSeq 500 sequencing ENA FIRST PUBLIC:2024 01 22|ENA LAST UPDATE:2024 01 22 1210F_S7_L002_R1_001.fastq.gz fastq 100538748.0 1339248.0 ena RUN TAB 15 01 2024 21:42:36:184 277004 0:75.07 A:36966184;C:19156780;G:21758079;T:22618911;N:38794 75       36966184 19156780 21758079 22618911 38794 ERX11852274 ERS17743538 ERA27788968 University of Birmingham|European Nucleotide Archive University of Birmingham                       B   usable mapping rate illumina nextseq unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2024-01-15 Pharyngula Embryo Embryo Imprecise All anatomical structures
15089 15089 ERR12476460 ERX11852281 ERS17743540 ERP156655 PRJEB71869 Effects of paternal starvation in the offspring development of zebrafish 33b9d14c-4211-4249-aede-f0f021d197e6 Other Dietary restriction in the form of fasting is a putative key to a healthier and longer life but these benefits may come at a trade off with reproductive fitness and may affect the following generations. The potential inter and transgenerational effects of long term fasting and starvation are particularly poorly understood in vertebrates when they originate from the paternal line. We utilised the externally fertilising zebrafish amenable to a split egg clutch design to explore the male specific effects of fasting/starvation on fertility and fitness of offspring independently of maternal contribution. Eighteen days of fasting resulted in reduced fertility in exposed males. While average offspring survival was not affected we detected increased larval growth rate in F1 offspring from starved males and more malformed embryos at 24 hpf in F2 offspring produced by F1 offspring from starved males. Comparing the transcriptomes of F1 embryos sired by starved and fed fathers revealed robust and reproducible increased expression of muscle composition genes but lower expression of lipid metabolism and lysosome genes in embryos from starved fathers. A large proportion of these genes showed enrichment in the yolk syncytial layer suggesting gene regulatory responses associated with metabolism of nutrients through paternal effects on extra embryonic tissues which are loaded with maternal factors. We compared the embryo transcriptomes to published adult transcriptome datasets and found comparable repressive effects of starvation on metabolism associated genes. These similarities suggest a physiologically relevant directed and potentially adaptive response transmitted by the father independently from the offspring's nutritional state which was defined by the mother. ENA FIRST PUBLIC:2024 01 15|ENA LAST UPDATE:2024 01 15   24 hpf embryo collected at 1219 from a cross with a fed father 1219 Fed 1219F   organism:Danio rerio|collection date:2018 02 02|scientific name:Danio rerio|common name:zebrafish|geographic location country and/or sea:Sweden                 NextSeq 500 sequencing ena EXPERIMENT TAB 15 01 2024 21:42:36:188 277017 1219F 1     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA NextSeq 500   ERP156655 NextSeq 500 sequencing ENA FIRST PUBLIC:2024 01 22|ENA LAST UPDATE:2024 01 22 1219F_S11_L001_R1_001.fastq.gz fastq 102219114.0 1358650.0 ena RUN TAB 15 01 2024 21:42:36:189 277018 0:75.24 A:35886099;C:19931420;G:22057068;T:24325918;N:18609 75       35886099 19931420 22057068 24325918 18609 ERX11852281 ERS17743540 ERA27788968 University of Birmingham|European Nucleotide Archive University of Birmingham                       B   usable mapping rate illumina nextseq unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2024-01-15 Pharyngula Embryo Embryo Imprecise All anatomical structures
15090 15090 ERR12476451 ERX11852272 ERS17743537 ERP156655 PRJEB71869 Effects of paternal starvation in the offspring development of zebrafish 33b9d14c-4211-4249-aede-f0f021d197e6 Other Dietary restriction in the form of fasting is a putative key to a healthier and longer life but these benefits may come at a trade off with reproductive fitness and may affect the following generations. The potential inter and transgenerational effects of long term fasting and starvation are particularly poorly understood in vertebrates when they originate from the paternal line. We utilised the externally fertilising zebrafish amenable to a split egg clutch design to explore the male specific effects of fasting/starvation on fertility and fitness of offspring independently of maternal contribution. Eighteen days of fasting resulted in reduced fertility in exposed males. While average offspring survival was not affected we detected increased larval growth rate in F1 offspring from starved males and more malformed embryos at 24 hpf in F2 offspring produced by F1 offspring from starved males. Comparing the transcriptomes of F1 embryos sired by starved and fed fathers revealed robust and reproducible increased expression of muscle composition genes but lower expression of lipid metabolism and lysosome genes in embryos from starved fathers. A large proportion of these genes showed enrichment in the yolk syncytial layer suggesting gene regulatory responses associated with metabolism of nutrients through paternal effects on extra embryonic tissues which are loaded with maternal factors. We compared the embryo transcriptomes to published adult transcriptome datasets and found comparable repressive effects of starvation on metabolism associated genes. These similarities suggest a physiologically relevant directed and potentially adaptive response transmitted by the father independently from the offspring's nutritional state which was defined by the mother. ENA FIRST PUBLIC:2024 01 15|ENA LAST UPDATE:2024 01 15   24 hpf embryo collected at 1203 from a cross with a starved father 1203 Starved 1203S   organism:Danio rerio|collection date:2018 02 02|scientific name:Danio rerio|common name:zebrafish|geographic location country and/or sea:Sweden                 NextSeq 500 sequencing ena EXPERIMENT TAB 15 01 2024 21:42:36:182 276999 1203S 1     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA NextSeq 500   ERP156655 NextSeq 500 sequencing ENA FIRST PUBLIC:2024 01 22|ENA LAST UPDATE:2024 01 22 1203S_S6_L004_R1_001.fastq.gz fastq 84880221.0 1129985.0 ena RUN TAB 15 01 2024 21:42:36:182 277000 0:75.12 A:30708531;C:16338743;G:18094345;T:19712906;N:25696 75       30708531 16338743 18094345 19712906 25696 ERX11852272 ERS17743537 ERA27788968 University of Birmingham|European Nucleotide Archive University of Birmingham                       B   usable mapping rate illumina nextseq unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2024-01-15 Pharyngula Embryo Embryo Imprecise All anatomical structures
15091 15091 ERR12476441 ERX11852262 ERS17743535 ERP156655 PRJEB71869 Effects of paternal starvation in the offspring development of zebrafish 33b9d14c-4211-4249-aede-f0f021d197e6 Other Dietary restriction in the form of fasting is a putative key to a healthier and longer life but these benefits may come at a trade off with reproductive fitness and may affect the following generations. The potential inter and transgenerational effects of long term fasting and starvation are particularly poorly understood in vertebrates when they originate from the paternal line. We utilised the externally fertilising zebrafish amenable to a split egg clutch design to explore the male specific effects of fasting/starvation on fertility and fitness of offspring independently of maternal contribution. Eighteen days of fasting resulted in reduced fertility in exposed males. While average offspring survival was not affected we detected increased larval growth rate in F1 offspring from starved males and more malformed embryos at 24 hpf in F2 offspring produced by F1 offspring from starved males. Comparing the transcriptomes of F1 embryos sired by starved and fed fathers revealed robust and reproducible increased expression of muscle composition genes but lower expression of lipid metabolism and lysosome genes in embryos from starved fathers. A large proportion of these genes showed enrichment in the yolk syncytial layer suggesting gene regulatory responses associated with metabolism of nutrients through paternal effects on extra embryonic tissues which are loaded with maternal factors. We compared the embryo transcriptomes to published adult transcriptome datasets and found comparable repressive effects of starvation on metabolism associated genes. These similarities suggest a physiologically relevant directed and potentially adaptive response transmitted by the father independently from the offspring's nutritional state which was defined by the mother. ENA FIRST PUBLIC:2024 01 15|ENA LAST UPDATE:2024 01 15   24 hpf embryo collected at 1118 from a cross with a starved father 1118 Starved 1118S   organism:Danio rerio|collection date:2018 02 02|scientific name:Danio rerio|common name:zebrafish|geographic location country and/or sea:Sweden                 NextSeq 500 sequencing ena EXPERIMENT TAB 15 01 2024 21:42:36:175 276979 1118S 1     RNA-Seq TRANSCRIPTOMIC cDNA SINGLE ILLUMINA NextSeq 500   ERP156655 NextSeq 500 sequencing ENA FIRST PUBLIC:2024 01 22|ENA LAST UPDATE:2024 01 22 1118S_S10_L002_R1_001.fastq.gz fastq 99340604.0 1321436.0 ena RUN TAB 15 01 2024 21:42:36:175 276980 0:75.18 A:34996267;C:19218610;G:21486623;T:23616635;N:22469 75       34996267 19218610 21486623 23616635 22469 ERX11852262 ERS17743535 ERA27788968 University of Birmingham|European Nucleotide Archive University of Birmingham                       B   usable mapping rate illumina nextseq unknown cdna_unspecified unknown bulk unknown unknown   United Kingdom 2024-01-15 Pharyngula Embryo Embryo Imprecise All anatomical structures

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CREATE TABLE run_metadata("run.accession" VARCHAR, "experiment.accession" VARCHAR, "sample.accession" VARCHAR, "study.accession" VARCHAR, bioproject VARCHAR, "study.title" VARCHAR, "study.alias" VARCHAR, "study.type" VARCHAR, "study.abstract" VARCHAR, "study.attributes" VARCHAR, "study.PMIDs" VARCHAR, "sample.description" VARCHAR, "sample.title" VARCHAR, "sample.alias" VARCHAR, "sample.centername" VARCHAR, "sample.attributes" VARCHAR, "GEOsample.title" VARCHAR, "GEOsample.dataprocessing" VARCHAR, "GEOsample.source" VARCHAR, "GEOsample.treatmentprotocol" VARCHAR, "GEOsample.extractprotocol" VARCHAR, "GEOsample.growthprotocol" VARCHAR, "GEOsample.characteristics" VARCHAR, "GEOsample.accession" VARCHAR, "experiment.title" VARCHAR, "experiment.alias" VARCHAR, "experiment.library_name" VARCHAR, "experiment.design_description" VARCHAR, "experiment.library_construction_protocol" VARCHAR, "experiment.attributes" VARCHAR, "experiment.library_strategy" VARCHAR, "experiment.library_source" VARCHAR, "experiment.library_selection" VARCHAR, "experiment.library_layout" VARCHAR, "experiment.platform" VARCHAR, "experiment.instrument_model" VARCHAR, "experiment.spot_descriptor" VARCHAR, "experiment.study_ref" VARCHAR, "run.title" VARCHAR, "run.attributes" VARCHAR, "run.filename" VARCHAR, "run.semantic_name" VARCHAR, "run.total_bases" DOUBLE, "run.total_spots" DOUBLE, "run.alias" VARCHAR, "run.read_lengths" VARCHAR, "run.base_counts" VARCHAR, "run.r1_length" BIGINT, "run.r2_length" BIGINT, "run.r3_length" BIGINT, "run.r4_length" BIGINT, "run.Acount" BIGINT, "run.Ccount" BIGINT, "run.Gcount" BIGINT, "run.Tcount" BIGINT, "run.Ncount" BIGINT, "run.experiment" VARCHAR, "run.pool_member" VARCHAR, "submission.accession" VARCHAR, "submission.srasource" VARCHAR, "submission.bioprojectsource" VARCHAR, "seqdetective.n_mates" BIGINT, "seqdetective.mapping_rate.mate1" DOUBLE, "seqdetective.mapping_rate.mate2" DOUBLE, "seqdetective.nofeature_rate.mate1" DOUBLE, "seqdetective.nofeature_rate.mate2" DOUBLE, "seqdetective.sparsity.mate1" DOUBLE, "seqdetective.sparsity.mate2" DOUBLE, "seqdetective.pos_strand_rate.mate1" DOUBLE, "seqdetective.pos_strand_rate.mate2" DOUBLE, "seqdetective.readlen.mate1" BIGINT, "seqdetective.readlen.mate2" BIGINT, "seqdetective.judgement.mate1" VARCHAR, "seqdetective.judgement.mate2" VARCHAR, "seqdetective.judgement.reason" VARCHAR, platform_family VARCHAR, instrument_generation VARCHAR, read_bias VARCHAR, selection_class VARCHAR, prep_kit VARCHAR, sc_or_bulk VARCHAR, tech_class VARCHAR, technology VARCHAR, tech_variant VARCHAR, "submission.bioprojectsource.country" VARCHAR, earliest_date DATE, devstage_curation VARCHAR, devstage_curation_coarse VARCHAR, tissue_curation VARCHAR, tissue_curation_coarse VARCHAR);;
CREATE INDEX idx_run_bioproject ON run_metadata(bioproject);;
CREATE INDEX idx_run_run_accession ON run_metadata("run.accession");;