{"database": "metadata", "table": "run_metadata", "rows": [[75388, "SRR24489457", "SRX20274763", "SRS17602458", "SRP436765", "PRJNA970658", "Transcriptome analysis of Cygb1 Knockout in Zebrafish Danio rerio", "PRJNA970658", "Other", "Cytoglobin Cygb is an evolutionary ancient heme protein with yet unclear physiological functions. Cygb might be involved in ROS detoxification  NO metabolism and lipid based signaling processes. Mammalian Cygb is ubiquitously expressed in all tissues. We used a CRISPR/Cas9 genome editing approach and generated a knockout of the penta coordinated cygb1 for in vivo analysis in zebrafish. Adult cygb1 knockouts develop phenotypic anomalies  including neoplasia of gonads  and weight loss. To identify the molecular mechanisms underlying the occurrence of these phenotypes and differentiate between function and effect of the knockout we compared the transcriptomes of cygb1 knockouts at different ages to age matched wild type WT zebrafish.", null, null, null, "Adult Brain F6", "F6 WT brain N R1 001", null, "strain:Tuebingen|isolate:WT Brain F6|age:12 mpf|dev stage:adult|sex:male|tissue:brain|genotype:wildtype|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNAseq Zebrafish 12mpf Brain WT", "F6 WT brain N", "F6 WT brain N", "Total RNA was extracted using the Crystal RNA Mini Kit following the instructions of the manufacturer. RNA samples were treated with DNaseI Qiagen  according to the manufacturers protocol. RNA concentration and quality for Illumina sequencing was measured by employing the TapeStation System 2200. A library for paired end sequencing was generated from 1 g total RNA.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina HiSeq 4000", null, "SRP436765", null, null, "F6-WT-brain-N_R1_001.fastq.gz F6-WT-brain-N_R2_001.fastq.gz", "fastq fastq", 5529906000.0, 18433020.0, "F6 WT brain N R1 001.fastq.gz", "0:150 1:150", "A:1484087685;C:1276380609;G:1344707858;T:1424369191;N:360657", 150, 150, null, null, 1484087685, 1276380609, 1344707858, 1424369191, 360657, "SRX20274763", "SRS17602458", "SRA1634682", "University of Hamburg|Institute of Animal Cell and Systems Biology (IZS)", "University of Hamburg", 2, 0.92807, 0.92821, 0.12062, 0.11965, 0.69126, 0.69702, 0.50832, 0.51172, 150, 150, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "Germany", "2023-05-10", "Adult", "Adult", "Brain", "Nervous System"]], "columns": ["rowid", "run.accession", "experiment.accession", "sample.accession", "study.accession", "bioproject", "study.title", "study.alias", "study.type", "study.abstract", "study.attributes", "study.PMIDs", "sample.description", "sample.title", "sample.alias", "sample.centername", "sample.attributes", "GEOsample.title", "GEOsample.dataprocessing", "GEOsample.source", "GEOsample.treatmentprotocol", "GEOsample.extractprotocol", "GEOsample.growthprotocol", "GEOsample.characteristics", "GEOsample.accession", "experiment.title", "experiment.alias", "experiment.library_name", "experiment.design_description", "experiment.library_construction_protocol", "experiment.attributes", "experiment.library_strategy", "experiment.library_source", "experiment.library_selection", "experiment.library_layout", "experiment.platform", "experiment.instrument_model", "experiment.spot_descriptor", "experiment.study_ref", "run.title", "run.attributes", "run.filename", "run.semantic_name", "run.total_bases", "run.total_spots", "run.alias", "run.read_lengths", "run.base_counts", "run.r1_length", "run.r2_length", "run.r3_length", "run.r4_length", "run.Acount", "run.Ccount", "run.Gcount", "run.Tcount", "run.Ncount", "run.experiment", "run.pool_member", "submission.accession", "submission.srasource", "submission.bioprojectsource", "seqdetective.n_mates", "seqdetective.mapping_rate.mate1", "seqdetective.mapping_rate.mate2", "seqdetective.nofeature_rate.mate1", "seqdetective.nofeature_rate.mate2", "seqdetective.sparsity.mate1", "seqdetective.sparsity.mate2", "seqdetective.pos_strand_rate.mate1", "seqdetective.pos_strand_rate.mate2", "seqdetective.readlen.mate1", "seqdetective.readlen.mate2", "seqdetective.judgement.mate1", "seqdetective.judgement.mate2", "seqdetective.judgement.reason", "platform_family", "instrument_generation", "read_bias", "selection_class", "prep_kit", "sc_or_bulk", "tech_class", "technology", "tech_variant", "submission.bioprojectsource.country", "earliest_date", "devstage_curation", "devstage_curation_coarse", "tissue_curation", "tissue_curation_coarse"], "primary_keys": ["rowid"], "primary_key_values": ["75388"], "units": {}, "query_ms": 12.628324999241158}