{"database": "metadata", "table": "run_metadata", "rows": [[74987, "SRR24211926", "SRX20008465", "SRS17352368", "SRP433358", "PRJNA957280", "Single cell resolution of the adult zebrafish intestine under conventional conditions  and in response to an acute  natural infection", "GSE230044", "Transcriptome Analysis", "We used single cell RNA sequencing to analyze the diversity of intestinal cells under conventional conditions and the effect of an acute  natural Vibrio cholerae infection on the intestinal transcriptome. Overall design: Adult zebrafish were infected with Vibrio cholerae for 16h then intestines dissected and dissociated into a single cell suspension and processed for single cell RNA sequencing.", null, "pubmed:37948182", null, "Intestine  Vibrio cholerae V52", "GSM7184373", null, "source name:Intestine|tissue:Intestine|age:9 mpf type:dissociated intestine|genotype:TL|treatment:Vibrio cholerae V52|indices:SI GA B8 AAAGTGCT GCTACCTG TGCTGTAA CTGCAAGC|geo loc name:missing|collection date:missing", "Intestine  Vibrio cholerae V52", "Raw base call files were demultiplexed and reads aligned to the Zebrafish reference genome Ensemble GRCz11 using Cell Ranger v3.0.2 Supplementary files format and content: barcodes and features as TSV files Supplementary files format and content: feature matrix as MTX file", "Intestine", null, "Intestinal tissue was dissociated enzymatically and mechanically followed by removal of debris and dead cells using OptiPrep\u2122 Density Gradient Medium Sigma. single cell RNA libraries from freshly collected cells were prepared and barcoded with a 10X genomics Chromium Next GEM Single Cell three prime Reagents Kit v3.", null, "tissue:Intestine|age:9 mpf type:dissociated intestine|genotype:TL|treatment:Vibrio cholerae V52|indices:SI GA B8 AAAGTGCT GCTACCTG TGCTGTAA CTGCAAGC", "GSM7184373", "GSM7184373: Intestine  Vibrio cholerae V52; 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