{"database": "metadata", "table": "run_metadata", "rows": [[74708, "SRR24003733", "SRX19807135", "SRS17168877", "SRP429947", "PRJNA949785", "Sequencing of zebrafish bone tissues", "PRJNA949785", "Other", "Transcriptomics was used to investigate sensitive genes associated with exercise improved bone mass", null, null, null, null, "eight month zebrafish bone tissues", null, "breed:Not applicable|age:8 month|sex:male|tissue:b1|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of b1 tissue in zebrafish", "control2 3", "control2 3", "Qualified total RNA was purified by DNase I  and mRNA was enriched by adding OligodT  attached magnetic beads. Subsequently  fragment buffer was added to cleave the mRNA into small   fragments. Fragmented mRNA was used as a template for reverse transcription to generate the first  cDNA strand  and then the second cDNA strand was generated. End repair and adaptor ligation were  performed by adding A tailing Mix and RNA Index Adapters  and then PCR amplification was performed. Single strand circle DNA library was obtained by recovering and cycling the PCR products.  The final library was obtained by purifying the linear DNA molecules that were not cycled. The quality  of the final library was tested. The qualified library was replicated by rolling circle replication to form  DNA nanospheres DNBs. DNBs were added to the mesh holes of a nanochip using high density DNA nanochip technology. Subsequently  sequencing was performed on the BGIISEQ500 platform  BGI Shenzhen  Guangdong  China using combined probe anchored polymerisation.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "PCR", "PAIRED", "BGISEQ", "BGISEQ-500", null, "SRP429947", null, null, "control2_3_1.fq.gz control2_3_2.fq.gz", "fastq fastq", 6683569500.0, 22278565.0, "control2 3 1.fq.gz", "0:150 1:150", "A:1846755716;C:1494194697;G:1482717957;T:1859901130;N:0", 150, 150, null, null, 1846755716, 1494194697, 1482717957, 1859901130, 0, "SRX19807135", "SRS17168877", "SRA1612461", "Sun Yat-sen University|Department of Epidemiology, School of Public Healt", "Sun Yat-sen University", 2, 0.93255, 0.93106, 0.10717, 0.10731, 0.67665, 0.67791, 0.54876, 0.52297, 150, 150, "B", "B", "biological fallback assumption", "bgi", "bgi", "unknown", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2023-03-29", "Adult", "Adult", "Bone or Cartilage", "Skeletal Element"]], "columns": ["rowid", "run.accession", "experiment.accession", "sample.accession", "study.accession", "bioproject", "study.title", "study.alias", "study.type", "study.abstract", "study.attributes", "study.PMIDs", "sample.description", "sample.title", "sample.alias", "sample.centername", "sample.attributes", "GEOsample.title", "GEOsample.dataprocessing", "GEOsample.source", "GEOsample.treatmentprotocol", "GEOsample.extractprotocol", "GEOsample.growthprotocol", "GEOsample.characteristics", "GEOsample.accession", "experiment.title", "experiment.alias", "experiment.library_name", "experiment.design_description", "experiment.library_construction_protocol", "experiment.attributes", "experiment.library_strategy", "experiment.library_source", "experiment.library_selection", "experiment.library_layout", "experiment.platform", "experiment.instrument_model", "experiment.spot_descriptor", "experiment.study_ref", "run.title", "run.attributes", "run.filename", "run.semantic_name", "run.total_bases", "run.total_spots", "run.alias", "run.read_lengths", "run.base_counts", "run.r1_length", "run.r2_length", "run.r3_length", "run.r4_length", "run.Acount", "run.Ccount", "run.Gcount", "run.Tcount", "run.Ncount", "run.experiment", "run.pool_member", "submission.accession", "submission.srasource", "submission.bioprojectsource", "seqdetective.n_mates", "seqdetective.mapping_rate.mate1", "seqdetective.mapping_rate.mate2", "seqdetective.nofeature_rate.mate1", "seqdetective.nofeature_rate.mate2", "seqdetective.sparsity.mate1", "seqdetective.sparsity.mate2", "seqdetective.pos_strand_rate.mate1", "seqdetective.pos_strand_rate.mate2", "seqdetective.readlen.mate1", "seqdetective.readlen.mate2", "seqdetective.judgement.mate1", "seqdetective.judgement.mate2", "seqdetective.judgement.reason", "platform_family", "instrument_generation", "read_bias", "selection_class", "prep_kit", "sc_or_bulk", "tech_class", "technology", "tech_variant", "submission.bioprojectsource.country", "earliest_date", "devstage_curation", "devstage_curation_coarse", "tissue_curation", "tissue_curation_coarse"], "primary_keys": ["rowid"], "primary_key_values": ["74708"], "units": {}, "query_ms": 8.443428996542934}