{"database": "metadata", "table": "run_metadata", "rows": [[74614, "SRR23868265", "SRX19680347", "SRS17049788", "SRP427394", "PRJNA944944", "Comparative transcriptome analysis of testes and ovaries reveals sex biased genes and pathways in zebrafish", "GSE227389", "Transcriptome Analysis", "The goals of this study are to compare the differentially expressed genes between testes and ovaries of zebrafish  based on RNA seq data and some of these genes were validated by qRT\u2013PCR.Further  the differentially expressed genes were devided into up regulated and down regulated genes for GO and KEGG analysis. Overall design: Testes and ovaries  mRNA profiles of adult  zebrafish were generated by deep sequencing. Every sample was compose of three  adult individuals.", null, "pubmed:38242380", null, "ovaries", "GSM7099752", null, "source name:ovary|tissue:ovary|genotype:WT|geo loc name:missing|collection date:missing", "ovaries", "Sequenced reads were trimmed for adaptor sequence  and masked for low complexity or low quality sequence  then mapped to GRCz10 whole genome using HISAT  mapped to genes using Bowtie2. fragments per kilo bases per million fragments FPKM were calculated using RSEM. Assembly: GRCz10 Supplementary files format and content: The text files include the Ensembl ID of genes and the FPKM values for each Sample.", "ovary", null, "Testes and ovaries were isolated  frozen on dry ice  and RNA was harvested using Trizol reagent. Agilent RNA 6000 nano Reagents Port 1 was used for RNA qualities analysis by Agilent 2100 Bioanalyzer. RNA libraries were prepared for sequencing using standard BGISEQ 500 protocols.", null, "tissue:ovary|genotype:WT", "GSM7099752", "GSM7099752: ovaries; Danio rerio; RNA Seq", "GSM7099752 r1", "GSM7099752", "1", "Testes and ovaries were isolated  frozen on dry ice  and RNA was harvested using Trizol reagent. Agilent RNA 6000 nano Reagents Port 1 was used for RNA qualities analysis by Agilent 2100 Bioanalyzer. RNA libraries were prepared for sequencing using standard BGISEQ 500 protocols.", null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "SINGLE", "BGISEQ", "BGISEQ-500", null, "SRP427394", null, null, "WT-ovary.fq.gz", "fastq", 1176463600.0, 23529272.0, "GSM7099752 r1", "0:50", "A:311869864;C:270650722;G:289863261;T:303489229;N:590524", 50, null, null, null, 311869864, 270650722, 289863261, 303489229, 590524, "SRX19680347", "SRS17049788", "SRA1687194", "Wuhan university", "Wuhan university", 1, 0.93488, null, 0.02755, null, 0.75601, null, 0.46986, null, 50, null, "B", null, "usable mapping rate", "bgi", "bgi", "unknown", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "China", "2023-03-15", "Undetermined", "Undetermined", "Gonad", "Reproductive System"]], "columns": ["rowid", "run.accession", "experiment.accession", "sample.accession", "study.accession", "bioproject", "study.title", "study.alias", "study.type", "study.abstract", "study.attributes", "study.PMIDs", "sample.description", "sample.title", "sample.alias", "sample.centername", "sample.attributes", "GEOsample.title", "GEOsample.dataprocessing", "GEOsample.source", "GEOsample.treatmentprotocol", "GEOsample.extractprotocol", "GEOsample.growthprotocol", "GEOsample.characteristics", "GEOsample.accession", "experiment.title", "experiment.alias", "experiment.library_name", "experiment.design_description", "experiment.library_construction_protocol", "experiment.attributes", "experiment.library_strategy", "experiment.library_source", "experiment.library_selection", "experiment.library_layout", "experiment.platform", "experiment.instrument_model", "experiment.spot_descriptor", "experiment.study_ref", "run.title", "run.attributes", "run.filename", "run.semantic_name", "run.total_bases", "run.total_spots", "run.alias", "run.read_lengths", "run.base_counts", "run.r1_length", "run.r2_length", "run.r3_length", "run.r4_length", "run.Acount", "run.Ccount", "run.Gcount", "run.Tcount", "run.Ncount", "run.experiment", "run.pool_member", "submission.accession", "submission.srasource", "submission.bioprojectsource", "seqdetective.n_mates", "seqdetective.mapping_rate.mate1", "seqdetective.mapping_rate.mate2", "seqdetective.nofeature_rate.mate1", "seqdetective.nofeature_rate.mate2", "seqdetective.sparsity.mate1", "seqdetective.sparsity.mate2", "seqdetective.pos_strand_rate.mate1", "seqdetective.pos_strand_rate.mate2", "seqdetective.readlen.mate1", "seqdetective.readlen.mate2", "seqdetective.judgement.mate1", "seqdetective.judgement.mate2", "seqdetective.judgement.reason", "platform_family", "instrument_generation", "read_bias", "selection_class", "prep_kit", "sc_or_bulk", "tech_class", "technology", "tech_variant", "submission.bioprojectsource.country", "earliest_date", "devstage_curation", "devstage_curation_coarse", "tissue_curation", "tissue_curation_coarse"], "primary_keys": ["rowid"], "primary_key_values": ["74614"], "units": {}, "query_ms": 12.605925992829725}