{"database": "metadata", "table": "run_metadata", "rows": [[74570, "SRR23829128", "SRX19650808", "SRS17019460", "SRP426991", "PRJNA944150", "Absence of telomerase restricts melanoma development.", "PRJNA944150", "Other", "Most cancers reactivate telomerase to maintain telomere length to acquire immortality. The importance of this process is well illustrated by the fact that telomerase promoter mutations are found at a high frequency in many cancer types  including melanoma. However  it is unclear when and if telomerase is strictly required during tumorigenesis. Here we show that melanoma can occur in the absence of telomerase. Nevertheless  it is required to sustain later growth and avoid regression. Using telomerase mutant zebrafish tert /  combined with two established melanoma models  we found equal melanoma incidence and invasiveness as tumors became visible. Later  however  while WT fish develop increasing larger tumors  tert /  tumors stagnate growth and regress. Consistently  tert /  tumors showed lower cell proliferation  higher apoptosis and melanocyte differentiation. We also detected an immune response to tert /  tumors. Indeed  tert /  tumors resumed growth when transplanted to immunocompromised hosts. We propose that  telomerase is required for melanoma in zebrafish  albeit at later stages of progression  to sustain growth while avoiding immune rejection and regression. Thus  absence of telomerase restricts melanoma through tumor autonomous mechanisms cell cycle arrest  apoptosis and melanocyte differentiation and a non tumor autonomous mechanisms immune rejection.", null, null, null, "2104/1", "17B", null, "strain:AB|age:10 month|sex:male|tissue:Tumor|birth date:24.06.2020|birth location:PEMAV  IRCAN France|death date:21.04.2021|genotype:tert+/+ rep5|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio rerio : 10 month male tumor", "17B", "17B", "RNA Seq of Danio rerio : 10 month male tumor", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "Oligo-dT", "PAIRED", "BGISEQ", "BGISEQ-500", null, "SRP426991", null, null, "17B_1.fq.gz 17B_2.fq.gz", "fastq fastq", 8787148400.0, 43935742.0, "17B 1.fq.gz", "0:100 1:100", "A:2617726378;C:1773416208;G:1829194025;T:2566811789;N:0", 100, 100, null, null, 2617726378, 1773416208, 1829194025, 2566811789, 0, "SRX19650808", "SRS17019460", "SRA1603485", "Sun Yat-sen University Cancer Center|State Key Laboratory of Oncology in South China", "Sun Yat-sen University Cancer Center Miguel Godinho Ferreira", 2, 0.84629, 0.85164, 0.49471, 0.49585, 0.79545, 0.79275, 0.64785, 0.57659, 100, 100, "B", "B", "biological fallback assumption", "bgi", "bgi", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "China", "2023-03-14", "Adult", "Adult", "Cancer or Tumor", "Cancer or Tumor"]], "columns": ["rowid", "run.accession", "experiment.accession", "sample.accession", "study.accession", "bioproject", "study.title", "study.alias", "study.type", "study.abstract", "study.attributes", "study.PMIDs", "sample.description", "sample.title", "sample.alias", "sample.centername", "sample.attributes", "GEOsample.title", "GEOsample.dataprocessing", "GEOsample.source", "GEOsample.treatmentprotocol", "GEOsample.extractprotocol", "GEOsample.growthprotocol", "GEOsample.characteristics", "GEOsample.accession", "experiment.title", "experiment.alias", "experiment.library_name", "experiment.design_description", "experiment.library_construction_protocol", "experiment.attributes", "experiment.library_strategy", "experiment.library_source", "experiment.library_selection", "experiment.library_layout", "experiment.platform", "experiment.instrument_model", "experiment.spot_descriptor", "experiment.study_ref", "run.title", "run.attributes", "run.filename", "run.semantic_name", "run.total_bases", "run.total_spots", "run.alias", "run.read_lengths", "run.base_counts", "run.r1_length", "run.r2_length", "run.r3_length", "run.r4_length", "run.Acount", "run.Ccount", "run.Gcount", "run.Tcount", "run.Ncount", "run.experiment", "run.pool_member", "submission.accession", "submission.srasource", "submission.bioprojectsource", "seqdetective.n_mates", "seqdetective.mapping_rate.mate1", "seqdetective.mapping_rate.mate2", "seqdetective.nofeature_rate.mate1", "seqdetective.nofeature_rate.mate2", "seqdetective.sparsity.mate1", "seqdetective.sparsity.mate2", "seqdetective.pos_strand_rate.mate1", "seqdetective.pos_strand_rate.mate2", "seqdetective.readlen.mate1", "seqdetective.readlen.mate2", "seqdetective.judgement.mate1", "seqdetective.judgement.mate2", "seqdetective.judgement.reason", "platform_family", "instrument_generation", "read_bias", "selection_class", "prep_kit", "sc_or_bulk", "tech_class", "technology", "tech_variant", "submission.bioprojectsource.country", "earliest_date", "devstage_curation", "devstage_curation_coarse", "tissue_curation", "tissue_curation_coarse"], "primary_keys": ["rowid"], "primary_key_values": ["74570"], "units": {}, "query_ms": 16.814289992908016}