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A 4sU injected control was added to the zebrafish samples to identify high turnover genes", "parent bioproject:PRJNA837863", "pubmed:36573354", null, "larvae  injected", "GSM6135029", null, "tissue:whole larvae  48hpf|cell type:whole larvae  48hpf|genotype:WT|treatment:30 min 4sU injected|age:48hpf", "larvae  injected", "Demultiplexing bcl2fastq v2.19  CELseq barcodes demultiplexed using custom code https://github.com/anikaneuschulz/CELseq3 demultiplex mapping using STAR v2.7.3a aggregation of mutation information and calculation of labeling rates per gene / in bulk using custom code https://github.com/anikaneuschulz/MTglob pipeline genome build/assembly: dr11.95 processed data files format and content: Tab delimited mutation rates per gene", "whole larvae  48hpf", null, "10 larvae injected with 5nl 4sU 100mM in 10 mM Tris\u2022HCl pH 7.4  Carl Roth with Dextran fluorescein Thermo Fisher Scientific #7136 were incubated at 28\u00b0C for 30 minutes  anesthesized on ice and homogenized in TRIzol RNA was extracted using TRIzol Chloroform Isopropanol according to the manufacturer\u2019s instructions. Iodiacetamide Treatment was carried out on extracted RNA in 50% DMSO as described in Herzog et. Al 2017 https://doi.org/10.1038/nmeth.4435. libraries for RNA seq were prepared following a CELseq protocol as published in Holler & Junker 2019 https://doi.org/10.1007/978 1 4939 9009 2 9. Sequencing adapters were switched \u2013 Insert is in read 1!", null, "cell type:whole larvae  48hpf|genotype:WT|treatment:30 min 4sU injected|age:48hpf", "GSM6135029", "GSM6135029: larvae  injected; Danio rerio; RNA Seq", "GSM6135029 r1", "GSM6135029", "1", "10 larvae injected with 5nl 4sU 100mM in 10 mM Tris\u2022HCl pH 7.4  Carl Roth with Dextran fluorescein Thermo Fisher Scientific #7136 were incubated at 28\u00b0C for 30 minutes  anesthesized on ice and homogenized in TRIzol RNA was extracted using TRIzol Chloroform Isopropanol according to the manufacturer's instructions. Iodiacetamide Treatment was carried out on extracted RNA in 50% DMSO as described in Herzog et. Al 2017 https://doi.org/10.1038/nmeth.4435. libraries for RNA seq were prepared following a CELseq protocol as published in Holler & Junker 2019 https://doi.org/10.1007/978 1 4939 9009 2 9. 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