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A 4sU injected control was added to the zebrafish samples to identify high turnover genes", "parent bioproject:PRJNA837863", "pubmed:36573354", null, "larvae  cold dissociated  rep 3", "GSM6735818", null, "tissue:whole larvae  48hpf|cell type:whole larvae  48hpf|genotype:WT|treatment:30 min 4sU during dissociation|time:48hpf", "larvae  cold dissociated  rep 3", "Demultiplexing bcl2fastq v2.19  CELseq barcodes demultiplexed using custom code https://github.com/anikaneuschulz/CELseq3 demultiplex mapping using STAR v2.7.3a aggregation of mutation information and calculation of labeling rates per gene / in bulk using custom code https://github.com/anikaneuschulz/MTglob pipeline genome build/assembly: dr11.95 processed data files format and content: Tab delimited mutation rates per gene", "whole larvae  48hpf", null, "10  anesthetized larvae were washed twice in ice cold HBSS Life Technologies GmbH #14175095 and resuspended in 200\u00b5l psychrophilic protease mix 1x Native Bacillus licheniformis Protease Creative Enzymes #NATE 0633 in HBSS  10 mM 4sU and incubated on ice for 30 min. Dissociation was aided by pipetting up and down with a 200 \u00b5l tip 25 times every 5 minutes. Enzymes were inactivated by adding 15 \u00b5l 10% BSA  Sigma Aldrich; #A4503 before spinning down and resuspending the cells in TRIzol RNA was extracted using TRIzol Chloroform Isopropanol according to the manufacturer\u2019s instructions. Iodiacetamide Treatment was carried out on extracted RNA in 50% DMSO as described in Herzog et. Al 2017 https://doi.org/10.1038/nmeth.4435. libraries for RNA seq were prepared following a CELseq protocol as published in Holler & Junker 2019 https://doi.org/10.1007/978 1 4939 9009 2 9. 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Al 2017 https://doi.org/10.1038/nmeth.4435. libraries for RNA seq were prepared following a CELseq protocol as published in Holler & Junker 2019 https://doi.org/10.1007/978 1 4939 9009 2 9. 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