{"database": "metadata", "table": "run_metadata", "rows": [[69021, "SRR18305959", "SRX14443371", "SRS12250384", "SRP363665", "PRJNA815356", "Gene expression data from lsd1 mutant zebrafish embryos", "GSE198476", "Transcriptome Analysis", "Gene expression of zebrafish lsd1 mutants and wildtype sibling tails were analyzed by RNA seq. Overall design: RNA Seq performed on 6 zebrafish lsd1 mutant and 5 wildtype sibling tails pooled in groups of 5 tails each  in triplicate at 24hpf. The Illumina NovaSeq 150 x 150 bp Sequencing 100 M read pairs sequencing protocol was used.", null, "pubmed:36594016", null, "17656X10", "GSM5949313", null, "source name:tail/posterior to yolk sac|age:24 hpf|genotype:36433 wildtype|tissue:tail/posterior to yolk sac", "17656X10", "The Illumina adapters were trimmed using cutadapt version 1.16. Fastq files were aligned to the genome using STAR version 2.7.2c. Differentially expressed genes were found using DESeq2 version 1.22.2 and the hciR package on Github. Assembly: GRCz11 Supplementary files format and content: Tab delimited text file containing gene id  log2FC  padj  and raw counts", "tail/posterior to yolk sac", "Tails frozen at  80C in RNA stabilization solution QIAGEN. Tails rinsed in 1X PBS prior to homogenization and lysis.", "Homogenization and total RNA isolation preformed according to PureLink RNA Micro kit Invitrogen 12183016. Homogenization was performed with a 18.5 gauge needle 10 times before proceeding directly to RNA isolation. Carrier RNA not utilized. Samples were DNase treated. 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