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Overall design: scRNAseq profiling of melanocyte stem cells isolated from WT adult zebrafish skin during melanocyte regeneration", null, "pubmed:37021774", null, "WT day x 1", "GSM5714327", null, "tissue:Tgmitfa:nlsEGFP FACS enriched cells|genotype:WT|day:0", "WT day x 1", "Base call files were generated from the Illumina NextSeq 500 Fastq files for each sample were generated from base call files using CellRanger's MkFastQ Alignment  annotation  and counting were performed using CellRanger Downstream QC  Dimenstion Reduction  UMAP generation  and DEG analysis were performed in Seuratv3 Genome build: Lawson lab zebrafish annotation V4.3.2 Supplementary files format and content: txt.gz fiiles saved from Seurat Objects", "Tgmitfa:nlsEGFP FACS enriched cells", "750nM Neocuproine applied for 24 hours between day x and Day 1 to ablate mature melanocytes", "Scales were removed  zebrafish skin cut and peeled off underlying musce  enzymatic and mechanical dissociation  and mitfa:nlsEGFP cells were enriched for during FACS  single cell barcoding using the 10X controller 10X three prime v3.1 scRNAseq library prep steps were followed Samples were sequenced on an Illumina NextSeq 500 using a 75 cycle PE kit", "Tgmitfa:nlsEGFP animals in either a WT or kitalf background were raised to 3 month 6 month of age", "genotype:WT|day:0", "GSM5714327", "GSM5714327: WT day x 1; Danio rerio; RNA Seq", "GSM5714327 r1", "GSM5714327", "1", "Scales were removed  zebrafish skin cut and peeled off underlying musce  enzymatic and mechanical dissociation  and mitfa:nlsEGFP cells were enriched for during FACS  single cell barcoding using the 10X controller 10X three prime v3.1 scRNAseq library prep steps were followed Samples were sequenced on an Illumina NextSeq 500 using a 75 cycle PE kit", null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "ILLUMINA", "NextSeq 500", null, "SRP349128", null, "assembly:Lawson lab zebrafish annotation V4.3.2|intentional duplicate", "WTD0A_possorted_genome_bam.bam", "10X Genomics bam file", 7628491300.0, 152569826.0, "GSM5714327 r1", "0:50", "A:2377995758;C:1521202402;G:1696947530;T:2030231260;N:2114350", 50, null, null, null, 2377995758, 1521202402, 1696947530, 2030231260, 2114350, "SRX13302159", "SRS11213384", "SRA1620868", "Craig Ceol, Molecular Medicine, UMass Medical School", "Craig Ceol, Molecular Medicine, UMass Medical School", 1, 0.90188, null, 0.1668, null, 0.82154, null, 0.599, null, 50, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "cdna_unspecified", "unknown", "sc", "single_cell_droplet", "10x", null, "United States", "2021-12-03", "Adult", "Adult", "Skin", "Surface Structure"]], "columns": ["rowid", "run.accession", "experiment.accession", "sample.accession", "study.accession", "bioproject", "study.title", "study.alias", "study.type", "study.abstract", "study.attributes", "study.PMIDs", "sample.description", "sample.title", "sample.alias", "sample.centername", "sample.attributes", "GEOsample.title", "GEOsample.dataprocessing", "GEOsample.source", "GEOsample.treatmentprotocol", "GEOsample.extractprotocol", "GEOsample.growthprotocol", "GEOsample.characteristics", "GEOsample.accession", "experiment.title", "experiment.alias", "experiment.library_name", "experiment.design_description", "experiment.library_construction_protocol", "experiment.attributes", "experiment.library_strategy", "experiment.library_source", "experiment.library_selection", "experiment.library_layout", "experiment.platform", "experiment.instrument_model", "experiment.spot_descriptor", "experiment.study_ref", "run.title", "run.attributes", "run.filename", "run.semantic_name", "run.total_bases", "run.total_spots", "run.alias", "run.read_lengths", "run.base_counts", "run.r1_length", "run.r2_length", "run.r3_length", "run.r4_length", "run.Acount", "run.Ccount", "run.Gcount", "run.Tcount", "run.Ncount", "run.experiment", "run.pool_member", "submission.accession", "submission.srasource", "submission.bioprojectsource", "seqdetective.n_mates", "seqdetective.mapping_rate.mate1", "seqdetective.mapping_rate.mate2", "seqdetective.nofeature_rate.mate1", "seqdetective.nofeature_rate.mate2", "seqdetective.sparsity.mate1", "seqdetective.sparsity.mate2", "seqdetective.pos_strand_rate.mate1", "seqdetective.pos_strand_rate.mate2", "seqdetective.readlen.mate1", "seqdetective.readlen.mate2", "seqdetective.judgement.mate1", "seqdetective.judgement.mate2", "seqdetective.judgement.reason", "platform_family", "instrument_generation", "read_bias", "selection_class", "prep_kit", "sc_or_bulk", "tech_class", "technology", "tech_variant", "submission.bioprojectsource.country", "earliest_date", "devstage_curation", "devstage_curation_coarse", "tissue_curation", "tissue_curation_coarse"], "primary_keys": ["rowid"], "primary_key_values": ["67334"], "units": {}, "query_ms": 6.911167001817375}