{"database": "metadata", "table": "run_metadata", "rows": [[66725, "SRR16490791", "SRX12693894", "SRS10644776", "SRP342165", "PRJNA772761", "Single cell transcriptomics reveals critical molecular programming of progenitors for motor neurons and oligodendrocytes in zebrafish", "GSE186163", "Transcriptome Analysis", "pmn progenitors and their offspring in spinal cords are isolated for sc RNAseq Overall design: olig2+ cells were isolated from the trunks of Tgolig2:dsred by FACS and underwent scRNA seq", null, "pubmed:36063998", null, "olig2+ cells in zebrafish trunks   28hpf", "GSM5639643", null, "source name:Tgolig2:dsred trunks|Stage:28 hpf|genotype:Tg olig2:dsred|tissue:trunk", "olig2+ cells in zebrafish trunks   28hpf", "Alignment  filtering  barcode counting  and UMI counting were performed with cellranger count module to generate feature barcode matrix and determine clusters. Genes detectable in more than two cells were defined as \u201cexpressed\u201d. Cells with <1000 genes detected were removed. Counts were normalized to 10000 and transformed into logarithmic scales. A neighborhood graph was embedded using UMAP displaying the top 4000 highly variable genes across cells. Cells were clustered by the Louvain Algorithm. The trajectory interface of all cell clusters was done using the layout 'fa'. These analyses were performed by the Python based SCANPY package Genome build: GRCz11 Supplementary files format and content: Matrix table with raw gene counts for every gene and every sample", "Tgolig2:dsred trunks", null, "Tgolig2:dsred trunks were dissected and dissociated with 0.25% trypsin. Fluorescent cells were isolated with FACS. RNA was extracted with Trizol. cDNAs were amplified and libraries were generated with the Single Cell 3\u2019 Library and Gel Bead kit V3", null, "Stage:28 hpf|genotype:Tg olig2:dsred|tissue:trunk", "GSM5639643", "GSM5639643: olig2+ cells in zebrafish trunks   28hpf; Danio rerio; RNA Seq", "GSM5639643 r1", "GSM5639643", "1", "Tgolig2:dsred trunks were dissected and dissociated with 0.25% trypsin. Fluorescent cells were isolated with FACS. 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