{"database": "metadata", "table": "run_metadata", "rows": [[62511, "SRR13234627", "SRX9666699", "SRS7865635", "SRP297464", "PRJNA683902", "Ythdf m6A readers function redundantly during zebrafish development", "PRJNA683902", "Other", "During the maternal to zygotic transition MZT  multiple mechanisms precisely control massive decay of maternal mRNAs. N6 methyladenosine m6A is known to regulate mRNA decay  yet how this modification promotes maternal transcript degradation remains unclear. Here  we find that m6A promotes maternal mRNA deadenylation. Yet  genetic loss of m6A readers Ythdf2 and Ythdf3 did not impact global maternal mRNA clearance  zygotic genome activation  or the onset of gastrulation  challenging the view that Ythdf2 alone is critical to developmental timing. We reveal that Ythdf proteins function redundantly during zebrafish oogenesis and development  as double Ythdf2 and Ythdf3 deletion prevented female gonad formation  and triple Ythdf mutants were lethal. Finally  we show that the microRNA miR 430 functions additively with methylation to promote degradation of common transcript targets. Together these findings reveal that m6A facilitates maternal mRNA deadenylation  and that multiple pathways and readers act in concert to mediate these effects of methylation on RNA stability.", null, null, null, "WT  MZdrosha  MZdicer mRNA 6  24  48 hpf  pA & R0   wild type 48hpf polyA", "WT  MZdrosha  MZdicer mRNA 6  24  48 hpf  pA & R0   WT 48h pA AGN000152", null, "strain:TU/AB|age:48.0|sex:pooled male and female|tissue:embryo|strain maternal:wild type|strain paternal:wild type|molecule:RNA|selection:pA|sample ref:AGS000140|replicate ref:AGN000152|replicate order:1|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "WT  MZdrosha  MZdicer mRNA 6  24  48 hpf  pA & R0   wild type 48hpf polyA", "AGR000206", "AGR000206", "RNA", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "unspecified", "SINGLE", "ILLUMINA", "Illumina HiSeq 2000", null, "SRP297464", null, null, "AGR000206_R1.fastq.gz", "fastq", 2629388112.0, 34597212.0, "AGR000206 R1.fastq.gz", "0:76 1:0", "A:705900102;C:587010373;G:584434557;T:751862507;N:180573", 76, 0, null, null, 705900102, 587010373, 584434557, 751862507, 180573, "SRX9666699", "SRS7865635", "SRA1169659", "Yale_Giraldez|Genetics", "Yale_Giraldez_Group", 1, 0.93679, null, 0.13092, null, 0.68521, null, 0.44617, null, 76, null, "B", null, "usable mapping rate", "illumina", "hiseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "United States", "2020-12-10", "Hatching", "Embryo", "Embryo Imprecise", "All anatomical structures"]], "columns": ["rowid", "run.accession", "experiment.accession", "sample.accession", "study.accession", "bioproject", "study.title", "study.alias", "study.type", "study.abstract", "study.attributes", "study.PMIDs", "sample.description", "sample.title", "sample.alias", "sample.centername", "sample.attributes", "GEOsample.title", "GEOsample.dataprocessing", "GEOsample.source", "GEOsample.treatmentprotocol", "GEOsample.extractprotocol", "GEOsample.growthprotocol", "GEOsample.characteristics", "GEOsample.accession", "experiment.title", "experiment.alias", "experiment.library_name", "experiment.design_description", "experiment.library_construction_protocol", "experiment.attributes", "experiment.library_strategy", "experiment.library_source", "experiment.library_selection", "experiment.library_layout", "experiment.platform", "experiment.instrument_model", "experiment.spot_descriptor", "experiment.study_ref", "run.title", "run.attributes", "run.filename", "run.semantic_name", "run.total_bases", "run.total_spots", "run.alias", "run.read_lengths", "run.base_counts", "run.r1_length", "run.r2_length", "run.r3_length", "run.r4_length", "run.Acount", "run.Ccount", "run.Gcount", "run.Tcount", "run.Ncount", "run.experiment", "run.pool_member", "submission.accession", "submission.srasource", "submission.bioprojectsource", "seqdetective.n_mates", "seqdetective.mapping_rate.mate1", "seqdetective.mapping_rate.mate2", "seqdetective.nofeature_rate.mate1", "seqdetective.nofeature_rate.mate2", "seqdetective.sparsity.mate1", "seqdetective.sparsity.mate2", "seqdetective.pos_strand_rate.mate1", "seqdetective.pos_strand_rate.mate2", "seqdetective.readlen.mate1", "seqdetective.readlen.mate2", "seqdetective.judgement.mate1", "seqdetective.judgement.mate2", "seqdetective.judgement.reason", "platform_family", "instrument_generation", "read_bias", "selection_class", "prep_kit", "sc_or_bulk", "tech_class", "technology", "tech_variant", "submission.bioprojectsource.country", "earliest_date", "devstage_curation", "devstage_curation_coarse", "tissue_curation", "tissue_curation_coarse"], "primary_keys": ["rowid"], "primary_key_values": ["62511"], "units": {}, "query_ms": 9.397234010975808}