{"database": "metadata", "table": "run_metadata", "rows": [[62492, "SRR13234608", "SRX9666718", "SRS7865655", "SRP297464", "PRJNA683902", "Ythdf m6A readers function redundantly during zebrafish development", "PRJNA683902", "Other", "During the maternal to zygotic transition MZT  multiple mechanisms precisely control massive decay of maternal mRNAs. N6 methyladenosine m6A is known to regulate mRNA decay  yet how this modification promotes maternal transcript degradation remains unclear. Here  we find that m6A promotes maternal mRNA deadenylation. Yet  genetic loss of m6A readers Ythdf2 and Ythdf3 did not impact global maternal mRNA clearance  zygotic genome activation  or the onset of gastrulation  challenging the view that Ythdf2 alone is critical to developmental timing. We reveal that Ythdf proteins function redundantly during zebrafish oogenesis and development  as double Ythdf2 and Ythdf3 deletion prevented female gonad formation  and triple Ythdf mutants were lethal. Finally  we show that the microRNA miR 430 functions additively with methylation to promote degradation of common transcript targets. Together these findings reveal that m6A facilitates maternal mRNA deadenylation  and that multiple pathways and readers act in concert to mediate these effects of methylation on RNA stability.", null, null, null, "MZythdf2 vs bkgd match WT mRNA pA & R0  rep1   MZythdf2 6hpf untreated ribo0", "MZythdf2 vs bkgd match WT mRNA pA & R0  rep1   MZythdf2 6h r0 AGN001793", null, "strain:TU/AB|age:6.0|dev stage:shield|sex:pooled male and female|tissue:embryo|genotype:MZythdf2  8/ 8|strain maternal:ythdf2  8/ 8|strain paternal:ythdf2  8/ 8|molecule:mRNA|selection:r0|sample ref:AGS001439|replicate ref:AGN001793|replicate order:1|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "MZythdf2 vs bkgd match WT mRNA pA & R0  rep1   MZythdf2 6hpf untreated ribo0", "AGR002453", "AGR002453", "mRNA", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "unspecified", "SINGLE", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP297464", null, null, "AGR002453_R1.fastq.gz", "fastq", 2027694820.0, 26680195.0, "AGR002453 R1.fastq.gz", "0:76 1:0", "A:419274134;C:549004017;G:566872772;T:492455226;N:88671", 76, 0, null, null, 419274134, 549004017, 566872772, 492455226, 88671, "SRX9666718", "SRS7865655", "SRA1169659", "Yale_Giraldez|Genetics", "Yale_Giraldez_Group", 1, 0.61548, null, 0.17157, null, 0.76986, null, 0.47549, null, 76, null, "B", null, "usable mapping rate", "illumina", "hiseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "United States", "2020-12-10", "Gastrula", "Embryo", "Embryo Imprecise", "All anatomical structures"]], "columns": ["rowid", "run.accession", "experiment.accession", "sample.accession", "study.accession", "bioproject", "study.title", "study.alias", "study.type", "study.abstract", "study.attributes", "study.PMIDs", "sample.description", "sample.title", "sample.alias", "sample.centername", "sample.attributes", "GEOsample.title", "GEOsample.dataprocessing", "GEOsample.source", "GEOsample.treatmentprotocol", "GEOsample.extractprotocol", "GEOsample.growthprotocol", "GEOsample.characteristics", "GEOsample.accession", "experiment.title", "experiment.alias", "experiment.library_name", "experiment.design_description", "experiment.library_construction_protocol", "experiment.attributes", "experiment.library_strategy", "experiment.library_source", "experiment.library_selection", "experiment.library_layout", "experiment.platform", "experiment.instrument_model", "experiment.spot_descriptor", "experiment.study_ref", "run.title", "run.attributes", "run.filename", "run.semantic_name", "run.total_bases", "run.total_spots", "run.alias", "run.read_lengths", "run.base_counts", "run.r1_length", "run.r2_length", "run.r3_length", "run.r4_length", "run.Acount", "run.Ccount", "run.Gcount", "run.Tcount", "run.Ncount", "run.experiment", "run.pool_member", "submission.accession", "submission.srasource", "submission.bioprojectsource", "seqdetective.n_mates", "seqdetective.mapping_rate.mate1", "seqdetective.mapping_rate.mate2", "seqdetective.nofeature_rate.mate1", "seqdetective.nofeature_rate.mate2", "seqdetective.sparsity.mate1", "seqdetective.sparsity.mate2", "seqdetective.pos_strand_rate.mate1", "seqdetective.pos_strand_rate.mate2", "seqdetective.readlen.mate1", "seqdetective.readlen.mate2", "seqdetective.judgement.mate1", "seqdetective.judgement.mate2", "seqdetective.judgement.reason", "platform_family", "instrument_generation", "read_bias", "selection_class", "prep_kit", "sc_or_bulk", "tech_class", "technology", "tech_variant", "submission.bioprojectsource.country", "earliest_date", "devstage_curation", "devstage_curation_coarse", "tissue_curation", "tissue_curation_coarse"], "primary_keys": ["rowid"], "primary_key_values": ["62492"], "units": {}, "query_ms": 7.96571999671869}