{"database": "metadata", "table": "run_metadata", "rows": [[61436, "SRR12764351", "SRX9235084", "SRS7468485", "SRP286165", "PRJNA667006", "Morphine alleviates pain post heart cryonjury in zebrafish without xxx regeneration", "GSE158919", "Transcriptome Analysis", "Nociceptive response belongs to a basic animal behavior facilitating adaptability and survival upon external or internal stimuli. Fish  similarly to higher vertebrates  also possess nociceptive machinery. Current protocols involving procedures performed on adult zebrafish including heart cryoinjury do not  however  take into account the adverse effects including pain that may potentially arise from these methodologies. Here  we assess the effect of two analgesics  lidocaine and morphine  followed post the heart cryoinjury in zebrafish. Monitoring swimming behaviour together with histology and gene expression analysis at the single cell level using scRNA sequencing and RNAscope fluorescent in situ hybridization technology  we show morphine  but not lidocaine  significantly improves animal welfare 6 hours post cryoinjury  without xxx heart regeneration process. Altogether  morphine should be considered as the analgesic of choice to reduce post surgical pain in adult zebrafish. Overall design: Single cell RNAseq of morphine treated and untreated zebrafish heart samples at 3  7 and 15 xxx post injury", null, "pubmed:35864193", null, "Injured heart Hr15", "GSM4815757", null, "source name:Zebrafish heart|tissue:heart|strain:AB|morphine treatment:yes", "Injured heart Hr15", "Demultiplexing of the illumina sequencing results using cellranger 3.0.2 Mapping using cellranger 3.0.2 Genome build: GRCz11 Supplementary files format and content: HDF5 Feature Barcode Matrix Format  Single cell transcript count table with list of cell barcodes and gene names", "Zebrafish heart", "Fish were either untreated or treated with 1.5mg/l morphine sulphate dissolved in system water. Fish were hold in tanks individually for the period of treatment of 6 hours.", "Single cell suspension were made using LiberaseTM in HBSS buffer Library were constructed following the manufacturer's protocols 10X Genomics", "Zebrafish were bred  raised  and maintained in accordance with the guidelines of the Max Delbr\u00fcck Center for Molecular Medicine and the local authority for animal protection Landesamt f\u00fcr Gesundheit und Soziales  Berlin  Germany for the use of laboratory animals  and followed the \u2018Principles of Laboratory Animal Care\u2019 NIH publication no. 86 23  revised 1985 as well as the current version of German Law on the Protection of Animals. Zebrafish strains\u00a0AB were used for cryoinjury procedure and behavioral analysis.", "tissue:heart|strain:AB|xxx post injury dpi:7|morphine treatment:yes", "GSM4815757", "GSM4815757: Injured heart Hr15; Danio rerio; RNA Seq", "GSM4815757", null, "1", "Single cell suspension were made using LiberaseTM in HBSS buffer Library were constructed following the manufacturer's protocols 10X Genomics", "GEO Accession:GSM4815757", "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "ILLUMINA", "Illumina HiSeq 4000", null, "SRP286165", null, null, "Hr15_7dpi_R1.fastq.gz Hr15_7dpi_R2.fastq.gz", "fastq fastq", 23195422020.0, 187059855.0, "GSM4815757 r1", "0:26 1:98", "A:6444667358;C:5404326039;G:5039988304;T:6295302014;N:11138305", 26, 98, null, null, 6444667358, 5404326039, 5039988304, 6295302014, 11138305, "SRX9235084", "SRS7468485", "SRA1136952", "GEO", "Junker lab, Berlin Institute for Medical Systems Biology, Max-Delbueck-Center Berlin", 2, 0.00189, 0.94386, 0.00063, 0.08495, 0.99705, 0.86468, 0.46558, 0.61998, 26, 98, "T", "B", "sc-like readlen", "illumina", "hiseq_era", "unknown", "cdna_unspecified", "unknown", "sc", "single_cell_droplet", "10x", null, "Germany", "2020-10-02", "Undetermined", "Adult", "Heart", "Cardiovascular System"]], "columns": ["rowid", "run.accession", "experiment.accession", "sample.accession", "study.accession", "bioproject", "study.title", "study.alias", "study.type", "study.abstract", "study.attributes", "study.PMIDs", "sample.description", "sample.title", "sample.alias", "sample.centername", "sample.attributes", "GEOsample.title", "GEOsample.dataprocessing", "GEOsample.source", "GEOsample.treatmentprotocol", "GEOsample.extractprotocol", "GEOsample.growthprotocol", "GEOsample.characteristics", "GEOsample.accession", "experiment.title", "experiment.alias", "experiment.library_name", "experiment.design_description", "experiment.library_construction_protocol", "experiment.attributes", "experiment.library_strategy", "experiment.library_source", "experiment.library_selection", "experiment.library_layout", "experiment.platform", "experiment.instrument_model", "experiment.spot_descriptor", "experiment.study_ref", "run.title", "run.attributes", "run.filename", "run.semantic_name", "run.total_bases", "run.total_spots", "run.alias", "run.read_lengths", "run.base_counts", "run.r1_length", "run.r2_length", "run.r3_length", "run.r4_length", "run.Acount", "run.Ccount", "run.Gcount", "run.Tcount", "run.Ncount", "run.experiment", "run.pool_member", "submission.accession", "submission.srasource", "submission.bioprojectsource", "seqdetective.n_mates", "seqdetective.mapping_rate.mate1", "seqdetective.mapping_rate.mate2", "seqdetective.nofeature_rate.mate1", "seqdetective.nofeature_rate.mate2", "seqdetective.sparsity.mate1", "seqdetective.sparsity.mate2", "seqdetective.pos_strand_rate.mate1", "seqdetective.pos_strand_rate.mate2", "seqdetective.readlen.mate1", "seqdetective.readlen.mate2", "seqdetective.judgement.mate1", "seqdetective.judgement.mate2", "seqdetective.judgement.reason", "platform_family", "instrument_generation", "read_bias", "selection_class", "prep_kit", "sc_or_bulk", "tech_class", "technology", "tech_variant", "submission.bioprojectsource.country", "earliest_date", "devstage_curation", "devstage_curation_coarse", "tissue_curation", "tissue_curation_coarse"], "primary_keys": ["rowid"], "primary_key_values": ["61436"], "units": {}, "query_ms": 10.265895005431958}