{"database": "metadata", "table": "run_metadata", "rows": [[60924, "SRR12658671", "SRX9139729", "SRS7381395", "SRP282672", "PRJNA663960", "Redundant mechanisms driven independently by RUNX1 and GATA2 for hematopoietic development", "GSE158099", "Transcriptome Analysis", "We used single cell RNA sequencing to investigate the expression and the heterogeneity of wild type and runx1 mutant cd41 GFPLow cd41GFP hematopoietic stem and progenitor cells at embryonic 2.5 dpf  dpf and larval stages 6  10 dpf 16 dpf. Overall design: Single cell RNA seq with FACS sorted cd41 GFPlow cells from wild type and runx1 /  at 2.5  6  10 dpf 16 dpf.", "parent bioproject:PRJNA663961", "pubmed:34492681", null, "cd41:GFPlow WT 16dpf", "GSM4792214", null, "tissue:sorted cd41:GFPlow|strain:EK|genotype/variation:WT  tgcd41:GFP|age:16 dpf|cell subset:sorted cd41:GFPlow", "cd41:GFPlow WT 16dpf", "Sequences from the Chromium platform were de multiplexed and aligned using CellRanger ver. 2.0.2 from 10x Genomics using custom zebrafish reference genome GRCz10 and transcript annotation reference GRCz10.87 with default parameters. Genome build: GRCz10 Supplementary files format and content: barcodes.tsv  genes.tsv  features.tsv  matrix.mtx", "sorted cd41:GFPlow", null, "Single cell: dissociation protocol: DOI: 10.1016/j.mex.2018.10.009. Single cells were captured using 10x Genomics Chromium. Single cell RNA seq libraries were prepared using Chromium Single Cell 3\u2019 Library & Gel Bead Kit v3. Cell lysis  cDNA preparation and library construction were prepared following the manufacturer's protocol.", null, "strain:EK|genotype/variation:WT  tgcd41:GFP|age:16 dpf|cell subset:sorted cd41:GFPlow", "GSM4792214", "GSM4792214: cd41:GFPlow WT 16dpf; Danio rerio; RNA Seq", "GSM4792214", null, "1", "Single cell: dissociation protocol: DOI: 10.1016/j.mex.2018.10.009. Single cells were captured using 10x Genomics Chromium. Single cell RNA seq libraries were prepared using Chromium Single Cell three prime Library & Gel Bead Kit v3. 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