{"database": "metadata", "table": "run_metadata", "rows": [[60230, "SRR12173039", "SRX8687802", "SRS6966466", "SRP270926", "PRJNA644883", "Transcriptomic analyses in brains of exposed F0 zebrafish and their unexposed F1 and F2 offspring post early life exposure to permethrin 10 microg/L", "GSE154020", "Transcriptome Analysis", "In the present study  zebrafish were exposed to permethrin during early life  and F1 and F2 generations were bred unexposed. Permethrin exposed F0 fish showed a hypoactive phenotype at maturity  whereas males from the F1 and F2 generations showed a decrease in anxiety like behavior. Thus  we performed transcriptomic changes to identify underlying molecular mechanisms. They showed limited overlap between sex and generations. In F0  genes and pathways related to glutamatergic synapse activity Gene Ontology and Reactome databases were significantly enriched and may explain the behavioral effects. In F1 and F2 generations  mechanisms are less clear as limited changes which could correlate with behavior were observed in the F1 generation. Overall design: RNA sequencing was performed on males and females from the F0  F1 and F2 generations 4 replicates treatment  4 replicates control for each sex and each generation", null, "pubmed:33752003", null, "159B", "GSM4661902", null, "source name:PH11|tissue:whole brain|age:adult|strain:AB line|generation:F2|Sex:male", "159B", "Base calling and prefiltering adaptor removal was performed by BGI STAR 2.7 for alignment of the reads to DanRer11 whole genome with default parameters Counts of reads per gene were obtained with HTSeq v0.11.2 gtf annotation file GRCz11.97 Use of DESeq2 v1.24.0 for differential gene expression analysis Genome build: DanRer11 Supplementary files format and content: csv file including raw counts obtained from htseq unnormalized.", "PH11", null, "Fish were euthanized  brains were dissected and flash frozen in liquid nitrogen  and RNA was extracted using TriPrep extraction kit Macherey Nagel. One microg of total RNA was sent to BGI Europe A/S for pair ended transcriptome sequencing on BGI Seq500 according to BGI standard protocol", null, "tissue:whole brain|age:adult|strain:AB line|generation:F2|Sex:M", "GSM4661902", "GSM4661902: 159B; Danio rerio; RNA Seq", "GSM4661902", null, "1", "Fish were euthanized  brains were dissected and flash frozen in liquid nitrogen  and RNA was extracted using TriPrep extraction kit Macherey Nagel. 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