{"database": "metadata", "table": "run_metadata", "rows": [[60119, "SRR13716310", "SRX10104893", "SRS8261917", "SRP270158", "PRJNA644214", "Danio rerio Transcriptome under lethal cold stress", "PRJNA644214", "Transcriptome Analysis", "The transcriptional regulations of fish upon a lethal cold stress and during the following rewarming are not well known. The purpose of this study is to characterize the gene expression dynamics of zebrafish larvae exposed to lethal cold stress and subsequent rewarming. Our data defined the survival and death pathways determining the survival of fish from the cold induced cellular or tissue damage. Modulating the activities of these pathways may enhance cold tolerance of the cold sensitive farmed fishes.", null, null, "The larvae at 96hpf were exposed to 10oC for xxxh in the presence of 5 \u03bcM PD0325901  replicate #3", "10C PD 3", "10C PD 3", null, "strain:AB|dev stage:Larvae|sex:not applicable|tissue:Whole body|biomaterial provider:Zongbin Cui  No. 7 Donghu South Road  Wuchang District  Wuhan  Hubei Province  China|treatment:Zebrafish larvae exposed to 10oC in the presence of 5 \u03bcM PD0325901|replicate:biological replicate 3|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "10C PD#3", "10C PD#3", "10C PD#3", "The RNA sample was sent to Biomarker Technologies http://www.biomarker.com.cn for library construction and RNA sequencing. Briefly  mRNA molecules were enriched using Oligo dT magnetic beads and randomly fragmented. First strand cDNA synthesis was performed using random hexamers. Second strand cDNAs were synthesized by adding dNTPs  RNase and DNA polymerase. The double stranded cDNAs were purified using AMPure XP beads. post end filling  A tailing and sequencing adaptor ligation  cDNA fragments of desired size were selected using AMPure XP beads. Finally  the sequencing library was generated by PCR amplification. Library quantification was performed using a Qubit 2.0 and the insert size of library was analyzed by an Agilent 2102 Bioanalyzer system.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "PCR", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP270158", null, null, "10C-PD-3_L3_1.fq.gz 10C-PD-3_L3_2.fq.gz a10C-PD-3_L1_1.fq.gz a10C-PD-3_L1_2.fq.gz", "fastq fastq fastq fastq", 6553566900.0, 21845223.0, "10C PD 3 L3 1.fq.gz", "0:150 1:150", "A:1711712888;C:1560862189;G:1598014292;T:1682939768;N:37763", 150, 150, null, null, 1711712888, 1560862189, 1598014292, 1682939768, 37763, "SRX10104893", "SRS8261917", "SRA1196464", "Chinese Academy of Sciences|Institute of Hydrobiology", "Chinese Academy of Sciences", 2, 0.92319, 0.92324, 0.0836, 0.08355, 0.67306, 0.67249, 0.48209, 0.47844, 150, 150, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2021-02-15", "Larval", "Larval", "Trunk", "Surface Structure"]], "columns": ["rowid", "run.accession", "experiment.accession", "sample.accession", "study.accession", "bioproject", "study.title", "study.alias", "study.type", "study.abstract", "study.attributes", "study.PMIDs", "sample.description", "sample.title", "sample.alias", "sample.centername", "sample.attributes", "GEOsample.title", "GEOsample.dataprocessing", "GEOsample.source", "GEOsample.treatmentprotocol", "GEOsample.extractprotocol", "GEOsample.growthprotocol", "GEOsample.characteristics", "GEOsample.accession", "experiment.title", "experiment.alias", "experiment.library_name", "experiment.design_description", "experiment.library_construction_protocol", "experiment.attributes", "experiment.library_strategy", "experiment.library_source", "experiment.library_selection", "experiment.library_layout", "experiment.platform", "experiment.instrument_model", "experiment.spot_descriptor", "experiment.study_ref", "run.title", "run.attributes", "run.filename", "run.semantic_name", "run.total_bases", "run.total_spots", "run.alias", "run.read_lengths", "run.base_counts", "run.r1_length", "run.r2_length", "run.r3_length", "run.r4_length", "run.Acount", "run.Ccount", "run.Gcount", "run.Tcount", "run.Ncount", "run.experiment", "run.pool_member", "submission.accession", "submission.srasource", "submission.bioprojectsource", "seqdetective.n_mates", "seqdetective.mapping_rate.mate1", "seqdetective.mapping_rate.mate2", "seqdetective.nofeature_rate.mate1", "seqdetective.nofeature_rate.mate2", "seqdetective.sparsity.mate1", "seqdetective.sparsity.mate2", "seqdetective.pos_strand_rate.mate1", "seqdetective.pos_strand_rate.mate2", "seqdetective.readlen.mate1", "seqdetective.readlen.mate2", "seqdetective.judgement.mate1", "seqdetective.judgement.mate2", "seqdetective.judgement.reason", "platform_family", "instrument_generation", "read_bias", "selection_class", "prep_kit", "sc_or_bulk", "tech_class", "technology", "tech_variant", "submission.bioprojectsource.country", "earliest_date", "devstage_curation", "devstage_curation_coarse", "tissue_curation", "tissue_curation_coarse"], "primary_keys": ["rowid"], "primary_key_values": ["60119"], "units": {}, "query_ms": 8.165735001966823}