{"database": "metadata", "table": "run_metadata", "rows": [[56576, "SRR10990705", "SRX7652107", "SRS6081571", "SRP246169", "PRJNA604017", "DGAT1 is a bona fide oncogene stimulating cell growth and suppressing oxidative stress while enabling fatty acid accumulation", "GSE144555", "Transcriptome Analysis", "Forced over expression of dgat1a using the minicoopR system increased the rate of tumour formation in tp53m214K/m214k; mitfa / ; nras G12D. This was through increased TOR signalling and alterations in key metabolic pathways. Overall design: Examination of the effect of dgat1a overexpression in NRAS driven Zebrafish Melanoma using the miniCoopR system.", null, null, null, "Tgmitfa:dgat1a Rep2", "GSM4290790", null, "source name:Tumour|strain:tp53m214K/m214k; mitfa / ; nras G12D|tissue:Melanoma|genotype:Tgmitfa:dgat1a", "Tgmitfa:dgat1a Rep2", "Adapters were trimmed from raw sequencing reads using Trimmomatics v0.32 Trimmed reads were aligned to the zebrafish genome Ensembl  GRCz11 using STAR v2.5.3 Reads that mapped to chromosomes 1 25 were retained Gene counts were determined using featureCounts v1.6.2 and differential expression analysis was performed using DESeq2 v1.14.1  using a adjusted p value cut off of <0.05 DESeq2 was used to generate log2 normalised variance stabilising transformed VST counts Genome build: Ensembl  GRCz11 Supplementary files format and content: fpm DGAT1 RNAseq GEO Supplementary files format and content: GFP DGAT1 allresults GEO Supplementary files format and content: VST DGAT1 RNAseq GEO", "Tumour", null, "Tumours were removed and RNA was harvested using Trizol reagent. RNA libraries were prepared for sequencing using standard Illumina protocols", "Age matched tumours were harvested at 10 12 weeks", "strain:tp53m214K/m214k; mitfa / ; nras G12D|tissue:Melanoma|genotype:Tgmitfa:dgat1a", "GSM4290790", "GSM4290790: Tgmitfa:dgat1a Rep2; Danio rerio; RNA Seq", "GSM4290790", null, "1", "Tumours were removed and RNA was harvested using Trizol reagent. 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