{"database": "metadata", "table": "run_metadata", "rows": [[55628, "SRR10586538", "SRX7267026", "SRS5761553", "SRP234693", "PRJNA593443", "A single cell transcriptome atlas of the developing zebrafish hindbrain [fgfr1]", "GSE141426", "Transcriptome Analysis", "RNA seq profile of transgenic hindbrains expressing heat shock induced constitutively activated fgfR1 Tghsp70:ca fgfr1 and compared to heat shocked counterparts Method: 22hpf embryos were heat shocked for 30min at 38.5\u00b0C and then incubated for 2hr at 28.5\u00b0C . post hindbrains were microdissected and the RNA was extracted. fgfR1 overexpression was checked by qPCR and samples were selected for sequencing Overall design: 4 control heat shoked samples and 4 constitutively active fgfr1 were deep sequenced", "parent bioproject:PRJNA593433", "pubmed:32094115", null, "CA fgfr1 1", "GSM4202676", null, "source name:Dissected hindbrain and surrounding tissues|tissue:Dissected hindbrain and surrounding tissues|Stage:24 25hpf|strain:Tghsp70:ca fgfr1|genotype:constitutively active fgfr1|growth:28.5\u00b0C until 22hpf  30min at 38.5\u00b0C  2hr 28.5\u00b0C", "CA fgfr1 1", "Reads were and aligned against zebrafish genome GRCz10 and Ensembl release 86 transcript annotations using STAR v2.5.1b via the transcript quantification software RSEM v1.2.31 RSEM estimated gene level counts were rounded to integers Genome build: GRCz10 Supplementary files format and content: Tab delimited counts matrix with row representing genes and columns representing samples.", "Dissected hindbrain and surrounding tissues", null, "Embryos were decorionated and deyolked in DMEM with high Glucose  no Glutamine  no Calcium 11530556  Gibco; hindbrains were micro dissected using 0.33mm micro fine sterile needles; RNA was isolated using Quick RNA Microprep kit Zymo Research. Samples were prepared with the Clontech SMARTer kit 634926  TaKaRa for cDNA amplification  followed by NexteraXT Illumina for library preparation.", null, "tissue:Dissected hindbrain and surrounding tissues|Stage:24 25hpf|strain:Tghsp70:ca fgfr1|genotype:constitutively active fgfr1|growth:28.5\u00b0C until 22hpf  30min at 38.5\u00b0C  2hr 28.5\u00b0C", "GSM4202676", "GSM4202676: CA fgfr1 1; Danio rerio; RNA Seq", "GSM4202676", null, "1", "Embryos were decorionated and deyolked in DMEM with high Glucose  no Glutamine  no Calcium 11530556  Gibco; hindbrains were micro dissected using 0.33mm micro fine sterile needles; RNA was isolated using Quick RNA Microprep kit Zymo Research. 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