{"database": "metadata", "table": "run_metadata", "rows": [[54798, "SRR10151807", "SRX6877386", "SRS5413168", "SRP222763", "PRJNA572587", "Characterization of T cells from the larval zebrafish tail via single cell RNAseq", "GSE137770", "Transcriptome Analysis", "We report single cell RNA sequencing of cells from the Tglck:GFP larval zebrafish tail  to identify cell types expressing the marker and to identify gene candidates related to T cell migration behaviors. We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells  in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.", null, "pubmed:32427565", null, "lckgfp s248", "GSM4087369", null, "source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf", "lckgfp s248", "STAR 2.5 Htseq 0.8.0 htseq count  m intersection nonempty   nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell  in tab separated format lckgfp counts.txt", "Danio rerio tail", "Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+", "Lysis was performed as described in Schaum et al.  Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification  and a Nextera based protocol was used for library preparation  as described in Schaum et al.  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