{"database": "metadata", "table": "run_metadata", "rows": [[52352, "SRR9182358", "SRX5954856", "SRS4865348", "SRP200180", "PRJNA545942", "Targeting redox metabolism: the perfect storm induced by acrylamide poisoning in the brain", "PRJNA545942", "Other", "Acrylamide is a recognized carcinogen that has strong neurotoxic effectseffects in humans and in experimental animals  although the molecular mechanisms underlying these neurotoxic effects are not completely understood. We studied acrylamide neurotoxicity in adult zebrafish using an integrated approach that included biochemical  transcriptomic  proteomic and metabolomic data. Brain samples from exposed and control zebrafish were subjected to proton NMR for metabolomic analysis  to MALDI TOF mass spectrometry for proteome analysis  and to high throughput RNA sequencing for transcriptomic analysis. We detected the formation of acrylamide adducts with thiol groups in the brain metabolome  and the accumulation of acrylamide conjugates and propionamide adducts in Cys residues of proteins. These combined effects resulted in a quasi complete depletion of glutathione and to the inactivation of different components of the thioredoxin system. Multi omic functional analyses identified microtubules  thioredoxin related proteins  transmembrane transport  redox metabolism and catalytic activity  as the cellular functions significantly altered by acrylamide in the fish brain. We propose that the combined loss of function of both redox metabolism related systems configure a perfect storm that explains many acrylamide neurotoxic effects  like the dysregulation of genes related to microtubules  presynaptic vesicle alteration  and behavioral alterations. We further propose an Adverse Outcome Pathway of acrylamide neurotoxicity  connecting the different toxic effects across different levels of organization  from molecular interactions to behavioral changes. Our mechanistical approach may help developing new treatments against the neurotoxic effects of acrylamideand of other neurotoxicants that may share its toxic mode of action.", null, null, null, "ZF Brain NAC.ACR NV 17", "NV 17", null, "strain:WT|dev stage:Adult|sex:male|tissue:Brain|treatment:N acetyl L cysteine \u2013 Acrylamide replicate 1|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA seq of Danio rerio: NAC ACR treatment adult male brain", "NV 17", "NV 17", "Zebrafish brain RNA was sent to the Vanderbilt Technologies for Advanced Genomics Vanderbilt University  Nashville  TN for library preparation and sequencing on the Illumina Novaseq platform", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM PCR", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP200180", null, null, "2465-NV-17-AGGATAGC-CCAAGGTT_S365_R1_001.fastq.gz 2465-NV-17-AGGATAGC-CCAAGGTT_S365_R2_001.fastq.gz", "fastq fastq", 10214864100.0, 34049547.0, "2465 NV 17 AGGATAGC CCAAGGTT S365 R1 001.fastq.gz", "0:150 1:150", "A:2930759198;C:2153599072;G:2292844182;T:2823524324;N:14137324", 150, 150, null, null, 2930759198, 2153599072, 2292844182, 2823524324, 14137324, "SRX5954856", "SRS4865348", "SRA893242", "Mississippi State University|Institute for Genomics, Biocomputing & Biotechnolo", "Mississippi State University", 2, 0.90601, 0.9096, 0.1939, 0.19302, 0.71153, 0.71058, 0.4986, 0.49859, 150, 150, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "United States", "2019-06-03", "Adult", "Adult", "Brain", "Nervous System"]], "columns": ["rowid", "run.accession", "experiment.accession", "sample.accession", "study.accession", "bioproject", "study.title", "study.alias", "study.type", "study.abstract", "study.attributes", "study.PMIDs", "sample.description", "sample.title", "sample.alias", "sample.centername", "sample.attributes", "GEOsample.title", "GEOsample.dataprocessing", "GEOsample.source", "GEOsample.treatmentprotocol", "GEOsample.extractprotocol", "GEOsample.growthprotocol", "GEOsample.characteristics", "GEOsample.accession", "experiment.title", "experiment.alias", "experiment.library_name", "experiment.design_description", "experiment.library_construction_protocol", "experiment.attributes", "experiment.library_strategy", "experiment.library_source", "experiment.library_selection", "experiment.library_layout", "experiment.platform", "experiment.instrument_model", "experiment.spot_descriptor", "experiment.study_ref", "run.title", "run.attributes", "run.filename", "run.semantic_name", "run.total_bases", "run.total_spots", "run.alias", "run.read_lengths", "run.base_counts", "run.r1_length", "run.r2_length", "run.r3_length", "run.r4_length", "run.Acount", "run.Ccount", "run.Gcount", "run.Tcount", "run.Ncount", "run.experiment", "run.pool_member", "submission.accession", "submission.srasource", "submission.bioprojectsource", "seqdetective.n_mates", "seqdetective.mapping_rate.mate1", "seqdetective.mapping_rate.mate2", "seqdetective.nofeature_rate.mate1", "seqdetective.nofeature_rate.mate2", "seqdetective.sparsity.mate1", "seqdetective.sparsity.mate2", "seqdetective.pos_strand_rate.mate1", "seqdetective.pos_strand_rate.mate2", "seqdetective.readlen.mate1", "seqdetective.readlen.mate2", "seqdetective.judgement.mate1", "seqdetective.judgement.mate2", "seqdetective.judgement.reason", "platform_family", "instrument_generation", "read_bias", "selection_class", "prep_kit", "sc_or_bulk", "tech_class", "technology", "tech_variant", "submission.bioprojectsource.country", "earliest_date", "devstage_curation", "devstage_curation_coarse", "tissue_curation", "tissue_curation_coarse"], "primary_keys": ["rowid"], "primary_key_values": ["52352"], "units": {}, "query_ms": 9.419722002348863}