{"database": "metadata", "table": "run_metadata", "rows": [[52149, "SRR8953738", "SRX5733571", "SRS4646935", "SRP193814", "PRJNA535392", "Transcriptome analysis and identification of insecticide metabolism related genes post exposure to insecticide in Sitobion avenae", "PRJNA535392", "Other", "Aphids causes serious lost in the production of wheat. Grain aphid  Sitobion avenae  is the dominant species of aphid in all wheat region of China  and this species is also considered resistant to a variety of insecticides  including imidacloprid and chlorpyrifos. However  the resistance and metabolize mechanism of insecticides for S. avenae is still unclear. This study employed transcriptome analysis to compare the expression patterns of stress response genes under imidacloprid and chlorpyrifos for 15min  3h and 36h'exposure. post compared insecticide treated samples of different time duration to control sample  we obtained 60 to 2267 Differential Express Unigenes DEUs  among these DEUs  31 790 unigenes was classified into 66 786 categories of GO function group and 24 to 760 DEUs could be mapped into 54 to 268 KEGG pathways. The expression of DEUs related to insecticide metabolism related genes were analyzed. In the insecticide metabolism related genes  cuticle protein is the largest group in DEUs  and the second largest is ABC transporter. Our study will facilitate molecular research on insecticide resistance in S. avenae  as well as in other wheat aphids.", null, null, "miR 430 /  embryos were injected with miR 430 rescued  20 embryos at shield stage were collected for RNA seq analsysis", "Rescued", "R6", null, "strain:AB|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:Hong Kong|age:6 hpf stage:shield stage|sex:pooled male and female|tissue:embyos|phenotype:MiR 430 / |sample type:embryos|treatment:Rescued by miR 430 injection|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Transcriptome analysis and identification of insecticidemetabolism related genes post exposure to insecticide in Sitobion avenae", "IM15min 1", "IM15min 1", "IM15min 1", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "PCR", "PAIRED", "ILLUMINA", "HiSeq X Ten", null, "SRP193814", null, null, "IM15min_1_S91_L004_R1_001.fastq.gz IM15min_1_S91_L004_R2_001.fastq.gz", "fastq fastq", 5908953744.0, 19566072.0, "IM15min 1 S91 L004 R1 001.fastq.gz", "0:151 1:151", "A:1720180168;C:1223518369;G:1218504877;T:1746709378;N:40952", 151, 151, null, null, 1720180168, 1223518369, 1218504877, 1746709378, 40952, "SRX5733571", "SRS4646935", "SRA879464", "Institute of Plant Protection and Agro-Products Safety|Anhui Academy of Agricultural Sciences", "Institute of Plant Protection and Agro-Products Safety", 2, 0.0, 2e-05, 0.0, 0.0, 1.0, 0.99995, null, 0.33333, 151, 151, "T", "T", "mates < 9% mapping rate", "illumina", "hiseq_era", "unknown", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2020-01-25", "Gastrula", "Embryo", "Whole Organism", "All anatomical structures"]], "columns": ["rowid", "run.accession", "experiment.accession", "sample.accession", "study.accession", "bioproject", "study.title", "study.alias", "study.type", "study.abstract", "study.attributes", "study.PMIDs", "sample.description", "sample.title", "sample.alias", "sample.centername", "sample.attributes", "GEOsample.title", "GEOsample.dataprocessing", "GEOsample.source", "GEOsample.treatmentprotocol", "GEOsample.extractprotocol", "GEOsample.growthprotocol", "GEOsample.characteristics", "GEOsample.accession", "experiment.title", "experiment.alias", "experiment.library_name", "experiment.design_description", "experiment.library_construction_protocol", "experiment.attributes", "experiment.library_strategy", "experiment.library_source", "experiment.library_selection", "experiment.library_layout", "experiment.platform", "experiment.instrument_model", "experiment.spot_descriptor", "experiment.study_ref", "run.title", "run.attributes", "run.filename", "run.semantic_name", "run.total_bases", "run.total_spots", "run.alias", "run.read_lengths", "run.base_counts", "run.r1_length", "run.r2_length", "run.r3_length", "run.r4_length", "run.Acount", "run.Ccount", "run.Gcount", "run.Tcount", "run.Ncount", "run.experiment", "run.pool_member", "submission.accession", "submission.srasource", "submission.bioprojectsource", "seqdetective.n_mates", "seqdetective.mapping_rate.mate1", "seqdetective.mapping_rate.mate2", "seqdetective.nofeature_rate.mate1", "seqdetective.nofeature_rate.mate2", "seqdetective.sparsity.mate1", "seqdetective.sparsity.mate2", "seqdetective.pos_strand_rate.mate1", "seqdetective.pos_strand_rate.mate2", "seqdetective.readlen.mate1", "seqdetective.readlen.mate2", "seqdetective.judgement.mate1", "seqdetective.judgement.mate2", "seqdetective.judgement.reason", "platform_family", "instrument_generation", "read_bias", "selection_class", "prep_kit", "sc_or_bulk", "tech_class", "technology", "tech_variant", "submission.bioprojectsource.country", "earliest_date", "devstage_curation", "devstage_curation_coarse", "tissue_curation", "tissue_curation_coarse"], "primary_keys": ["rowid"], "primary_key_values": ["52149"], "units": {}, "query_ms": 12.155518001236487}