{"database": "metadata", "table": "run_metadata", "rows": [[51707, "SRR8791414", "SRX5581197", "SRS4542778", "SRP189574", "PRJNA529372", "miR 202 3p is important for the early development of zebrafish embryos.", "PRJNA529372", "Other", "The expression level of miR 202 in embryos is very low. However  when knocking out miR 202 using CRISPR/Cas9  it was found that homozygous embryos died early. Further experimental research found that miR 202 3p plays a crucial role. Therefore  we selected wild type  heterozygous and homozygous at 3.5hpf for RNA sequencing  hoping to explain the important regulation of miR 202 3p on early development of embryo.", null, null, null, "dre wide type 2", "sample 6", null, "strain:not applicable|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|age:not applicable|dev stage:3.5 hpf|sex:not applicable|tissue:embryo|collected by:Haoyu RAN|genotype:wide type|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "dre wide type 2", "202 WT 2", "202 WT 2", "The first step in the workflow involves purifying the poly A containing mRNA molecules using poly T oligo attached magnetic beads. Following purification  the mRNA is fragmented into small pieces using divalent cations under elevated temperature. The cleaved RNA fragments are copied into first strand cDNA using reverse transcriptase and random primers. Strand specificity is achieved by replacing dTTP with dUTP in the Second Strand Marking Mix SMM  followed by second strand cDNA synthesis using DNA Polymerase I and RNase H. The incorporation of dUTP in second strand synthesis quenches the second strand during amplification  because the polymerase used in the assay is not incorporated past this nucleotide. The addition of Actinomycin D to First Stand Synthesis Act D mix FSA prevents spurious DNA dependent synthesis  while allowing RNA dependent synthesis  improving strand specificity. These cDNA fragments then have the addition of a single 'A' base and subsequent ligation of the adapter. The products are then purified and enriched with PCR to create the final cDNA library.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "Oligo-dT", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP189574", null, null, "WT_3_5_9_S9_L001_R1_001.fastq.gz WT_3_5_9_S9_L001_R2_001.fastq.gz WT_3_5_9_S9_L002_R1_001.fastq.gz WT_3_5_9_S9_L002_R2_001.fastq.gz", "fastq fastq fastq fastq", 3728359666.0, 18551655.0, "WT 3 5 9 S9 L001 R1 001.fastq.gz", "0:100.50 1:100.48", "A:954293388;C:880356649;G:889396693;T:997281530;N:7031406", 100, 100, null, null, 954293388, 880356649, 889396693, 997281530, 7031406, "SRX5581197", "SRS4542778", "SRA866342", "SHANGHAI OCEAN UNIVERSITY|College of Fisheries and Life Science", "SHANGHAI OCEAN UNIVERSITY", 2, 0.9294, 0.91719, 0.03104, 0.03129, 0.75483, 0.76315, 0.48625, 0.48351, 101, 101, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "China", "2020-04-01", "Blastula", "Embryo", "Embryo Imprecise", "All anatomical structures"]], "columns": ["rowid", "run.accession", "experiment.accession", "sample.accession", "study.accession", "bioproject", "study.title", "study.alias", "study.type", "study.abstract", "study.attributes", "study.PMIDs", "sample.description", "sample.title", "sample.alias", "sample.centername", "sample.attributes", "GEOsample.title", "GEOsample.dataprocessing", "GEOsample.source", "GEOsample.treatmentprotocol", "GEOsample.extractprotocol", "GEOsample.growthprotocol", "GEOsample.characteristics", "GEOsample.accession", "experiment.title", "experiment.alias", "experiment.library_name", "experiment.design_description", "experiment.library_construction_protocol", "experiment.attributes", "experiment.library_strategy", "experiment.library_source", "experiment.library_selection", "experiment.library_layout", "experiment.platform", "experiment.instrument_model", "experiment.spot_descriptor", "experiment.study_ref", "run.title", "run.attributes", "run.filename", "run.semantic_name", "run.total_bases", "run.total_spots", "run.alias", "run.read_lengths", "run.base_counts", "run.r1_length", "run.r2_length", "run.r3_length", "run.r4_length", "run.Acount", "run.Ccount", "run.Gcount", "run.Tcount", "run.Ncount", "run.experiment", "run.pool_member", "submission.accession", "submission.srasource", "submission.bioprojectsource", "seqdetective.n_mates", "seqdetective.mapping_rate.mate1", "seqdetective.mapping_rate.mate2", "seqdetective.nofeature_rate.mate1", "seqdetective.nofeature_rate.mate2", "seqdetective.sparsity.mate1", "seqdetective.sparsity.mate2", "seqdetective.pos_strand_rate.mate1", "seqdetective.pos_strand_rate.mate2", "seqdetective.readlen.mate1", "seqdetective.readlen.mate2", "seqdetective.judgement.mate1", "seqdetective.judgement.mate2", "seqdetective.judgement.reason", "platform_family", "instrument_generation", "read_bias", "selection_class", "prep_kit", "sc_or_bulk", "tech_class", "technology", "tech_variant", "submission.bioprojectsource.country", "earliest_date", "devstage_curation", "devstage_curation_coarse", "tissue_curation", "tissue_curation_coarse"], "primary_keys": ["rowid"], "primary_key_values": ["51707"], "units": {}, "query_ms": 12.885179006843828}