{"database": "metadata", "table": "run_metadata", "rows": [[51249, "SRR8599810", "SRX5399817", "SRS4386660", "SRP186433", "PRJNA523477", "The alternative splicing regulator Nova2 constrains vascular Erk signalling to limit specification of the lymphatic lineage", "PRJNA523477", "Other", "The correct assignment of cell fate within fields of multipotent progenitors is essential for accurate tissue diversification. The first lymphatic vessels arise from pre existing veins post venous endothelial cells become specified as lymphatic progenitors. Prox1 specifies lymphatic fate and labels these progenitors  however the mechanisms restricting Prox1 expression and limiting the progenitor pool remain unknown. We identified a zebrafish mutant that displayed premature  expanded and prolonged lymphatic specification. The gene responsible encodes the regulator of alternative splicing  Nova2. In zebrafish and human endothelial cells  Nova2 selectively regulates pre mRNA splicing for components of signalling pathways and phosphoproteins. Nova2 deficient endothelial cells display increased Mapk/Erk signalling and Prox1 expression is dynamically controlled by Erk signalling. We identify a mechanism whereby Nova2 regulated splicing constrains Erk signalling  thus limiting lymphatic progenitor cell specification. This identifies the capacity of a factor that tunes mRNA splicing to control assignment of cell fate during vascular differentiation.", null, null, null, "sib1 S1 R1", "sib1 S1 R1", null, "isolate:sib FACS1|age:N/A|sex:N/A|tissue:lymphatic|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "sib1 S1 R1 Danio  rerio", "sib1 S1 R1", "sib1 S1 R1", "sib1 S1 R1", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "ILLUMINA", "NextSeq 500", null, "SRP186433", null, null, "sib1_S1_R1_001.fastq.gz sib1_S1_R2_001.fastq.gz", "fastq fastq", 8260538032.0, 54746632.0, "sib1 S1 R1 001.fastq.gz", "0:75.48 1:75.41", "A:2109874601;C:2016630266;G:2030604420;T:2100518772;N:2909973", 75, 75, null, null, 2109874601, 2016630266, 2030604420, 2100518772, 2909973, "SRX5399817", "SRS4386660", "SRA851016", "Salk Institute|Salk", "Salk Institute", 2, 0.9274, 0.93049, 0.04415, 0.04306, 0.72819, 0.73397, 0.45755, 0.4619, 76, 76, "B", "B", "biological fallback assumption", "illumina", "nextseq", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "United States", "2019-02-23", "Undetermined", "Undetermined", "Lymphatic System", "Cardiovascular System"]], "columns": ["rowid", "run.accession", "experiment.accession", "sample.accession", "study.accession", "bioproject", "study.title", "study.alias", "study.type", "study.abstract", "study.attributes", "study.PMIDs", "sample.description", "sample.title", "sample.alias", "sample.centername", "sample.attributes", "GEOsample.title", "GEOsample.dataprocessing", "GEOsample.source", "GEOsample.treatmentprotocol", "GEOsample.extractprotocol", "GEOsample.growthprotocol", "GEOsample.characteristics", "GEOsample.accession", "experiment.title", "experiment.alias", "experiment.library_name", "experiment.design_description", "experiment.library_construction_protocol", "experiment.attributes", "experiment.library_strategy", "experiment.library_source", "experiment.library_selection", "experiment.library_layout", "experiment.platform", "experiment.instrument_model", "experiment.spot_descriptor", "experiment.study_ref", "run.title", "run.attributes", "run.filename", "run.semantic_name", "run.total_bases", "run.total_spots", "run.alias", "run.read_lengths", "run.base_counts", "run.r1_length", "run.r2_length", "run.r3_length", "run.r4_length", "run.Acount", "run.Ccount", "run.Gcount", "run.Tcount", "run.Ncount", "run.experiment", "run.pool_member", "submission.accession", "submission.srasource", "submission.bioprojectsource", "seqdetective.n_mates", "seqdetective.mapping_rate.mate1", "seqdetective.mapping_rate.mate2", "seqdetective.nofeature_rate.mate1", "seqdetective.nofeature_rate.mate2", "seqdetective.sparsity.mate1", "seqdetective.sparsity.mate2", "seqdetective.pos_strand_rate.mate1", "seqdetective.pos_strand_rate.mate2", "seqdetective.readlen.mate1", "seqdetective.readlen.mate2", "seqdetective.judgement.mate1", "seqdetective.judgement.mate2", "seqdetective.judgement.reason", "platform_family", "instrument_generation", "read_bias", "selection_class", "prep_kit", "sc_or_bulk", "tech_class", "technology", "tech_variant", "submission.bioprojectsource.country", "earliest_date", "devstage_curation", "devstage_curation_coarse", "tissue_curation", "tissue_curation_coarse"], "primary_keys": ["rowid"], "primary_key_values": ["51249"], "units": {}, "query_ms": 15.167609999480192}