{"database": "metadata", "table": "run_metadata", "rows": [[50513, "SRR8293427", "SRX5108026", "SRS4117494", "SRP172911", "PRJNA498365", "Raw sequence reads  in the unfertilized eggs of zebrafish Danio rerio", "PRJNA498365", "Other", "Raw sequence reads of full length transcriptome sequencing in the unfertilized eggs of zebrafish Danio rerio.", null, null, "Three female zebrafish were selected and 100 unfertilized eggs from each female were harvested as three samples Z1 1  Z1 2  and Z1 3 respectively; Identical quantities of RNA from Z1 1  Z1 2  and Z1 3 were combined into sample Z1 M.", "Model organism or animal sample from Danio rerio", "unfertilized eggs from three female zebrafish were harvested as samples Z1 M", null, "isolate:3 female zebrafish were selected and 100 unfertilized eggs from each female were harvested as 3 samples Z1 1  Z1 2  and Z1 3 respectively; and identical quantities of RNA from Z1 1  Z1 2  and Z1 3 were combined into sample Z1 M.|dev stage:unfertilized egg|sex:female|tissue:ovary|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Full length transcriptome sequencing of zebrafish Danio rerio : adult female unfertilized eggs.", "1 2K D05 1", "1 2K D05 1", "RNA sample Z1 M was reverse transcribed into cDNA. Four fragment ranges 0.5 1K  1 2K  2 3K  and >3K were prepared according to the PacBio Iso Seq protocol. PacBio RS II sequencing reactions of 16 SMRT single molecule real time cells 3 cells of 0.5 1K  5 cells of 1 2K  5 cells of 2 3K  3 cells of >3K were performed in DNA Sequencing Reagent 4.0 Clontech  Mountain View  California  USA.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM PCR", "SINGLE", "PACBIO_SMRT", "PacBio RS II", null, "SRP172911", null, null, "m160831_064528_42199_c101016682550000001823228410211623_s1_p0.1.bax.h5 m160831_064528_42199_c101016682550000001823228410211623_s1_p0.2.bax.h5 m160831_064528_42199_c101016682550000001823228410211623_s1_p0.3.bax.h5 m160831_064528_42199_c101016682550000001823228410211623_s1_p0.bas.h5", "pacbio_native pacbio_native pacbio_native pacbio_native", 4494598972.0, 163482.0, "1 2K D05 1.tar.gz", null, "A:1263523570;C:919488657;G:1005901638;T:1241749988;N:0", null, null, null, null, 1263523570, 919488657, 1005901638, 1241749988, 0, "SRX5108026", "SRS4117494", "SRA820074", "Institute of Hydrobiology, Chinese Academy of Sciences|State Key Laboratory of Freshwater Ecology and Bio", "Institute of Hydrobiology, Chinese Academy of Sciences", null, null, null, null, null, null, null, null, null, null, null, null, null, null, "pacbio", "pacbio_early", "full_length", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2018-12-10", "Zygote", "Embryo", "Gonad", "Reproductive System"]], "columns": ["rowid", "run.accession", "experiment.accession", "sample.accession", "study.accession", "bioproject", "study.title", "study.alias", "study.type", "study.abstract", "study.attributes", "study.PMIDs", "sample.description", "sample.title", "sample.alias", "sample.centername", "sample.attributes", "GEOsample.title", "GEOsample.dataprocessing", "GEOsample.source", "GEOsample.treatmentprotocol", "GEOsample.extractprotocol", "GEOsample.growthprotocol", "GEOsample.characteristics", "GEOsample.accession", "experiment.title", "experiment.alias", "experiment.library_name", "experiment.design_description", "experiment.library_construction_protocol", "experiment.attributes", "experiment.library_strategy", "experiment.library_source", "experiment.library_selection", "experiment.library_layout", "experiment.platform", "experiment.instrument_model", "experiment.spot_descriptor", "experiment.study_ref", "run.title", "run.attributes", "run.filename", "run.semantic_name", "run.total_bases", "run.total_spots", "run.alias", "run.read_lengths", "run.base_counts", "run.r1_length", "run.r2_length", "run.r3_length", "run.r4_length", "run.Acount", "run.Ccount", "run.Gcount", "run.Tcount", "run.Ncount", "run.experiment", "run.pool_member", "submission.accession", "submission.srasource", "submission.bioprojectsource", "seqdetective.n_mates", "seqdetective.mapping_rate.mate1", "seqdetective.mapping_rate.mate2", "seqdetective.nofeature_rate.mate1", "seqdetective.nofeature_rate.mate2", "seqdetective.sparsity.mate1", "seqdetective.sparsity.mate2", "seqdetective.pos_strand_rate.mate1", "seqdetective.pos_strand_rate.mate2", "seqdetective.readlen.mate1", "seqdetective.readlen.mate2", "seqdetective.judgement.mate1", "seqdetective.judgement.mate2", "seqdetective.judgement.reason", "platform_family", "instrument_generation", "read_bias", "selection_class", "prep_kit", "sc_or_bulk", "tech_class", "technology", "tech_variant", "submission.bioprojectsource.country", "earliest_date", "devstage_curation", "devstage_curation_coarse", "tissue_curation", "tissue_curation_coarse"], "primary_keys": ["rowid"], "primary_key_values": ["50513"], "units": {}, "query_ms": 12.62360699911369}