{"database": "metadata", "table": "run_metadata", "rows": [[49042, "SRR7633492", "SRX4497223", "SRS3618223", "SRP155992", "PRJNA483985", "Transcriptomic analysis of isolated zebrafish podocytes and endothelial cells exposed to human APOL1 mRNA", "GSE118000", "Transcriptome Analysis", "We injected zebrafish embryos with human APOL1 mRNA  dissociated embryos at xxx days of age  and purified podocytes and endothelial cells with fluorescence activated cell sorting. post sorting  mRNA was extracted from purified cells and used to generate cDNA libraries  which were sequenced on an Illumina HiSeq 2500. Overall design: Examination of two cell types exposed to one of 3 mRNA treatments sham  APOL1 G0  APOL1 G2", null, "pubmed:31158233", null, "G2 endo 01", "GSM3317064", null, "tissue:zebrafish endothelium|line:fli:EGFP|treatment:human APOL1 G2 mRNA", "G2 endo 01", "Quality control analysis of each sequenced library was performed using fastQC version 0.11.7 Removal of primer adapters was performed with Trim Galore version 0.4.0 Trimmed sequencing reads were aligned and mapped using to the D. rerio genome release GRCm38 using the STAR version 2.6.0c reads were filtered  sorted and indexed with Samtools version 1.3 Uniquely mapped reads were used to generate counts for each annotated gene using HTSeq 0.10.0 read counts were analyzed with DESeq2 Genome build: GRCm38 Supplementary files format and content: .count files contain raw unnormalized read counts for input to DESeq2", "zebrafish endothelium", "Zebrafsih were injected with 1nL of either APOL1 mRNA 150pg/nL  or phenol red", "RNA was extracted from purified cells using a Qiagen RNeasy micro kit cDNA libraries were prepared using the SMARTSeq v.4 Ultra Low Input RNA Kit Clonetech", "Zebrafish were injected immediately post fertilization single cell stage and enbryos were harvested 4 dpf", "line:fli:EGFP|treatment:human APOL1 G2 mRNA", "GSM3317064", "GSM3317064: G2 endo 01; Danio rerio; RNA Seq", "GSM3317064", null, "1", "RNA was extracted from purified cells using a Qiagen RNeasy micro kit cDNA libraries were prepared using the SMARTSeq v.4 Ultra Low Input RNA Kit Clonetech", "GEO Accession:GSM3317064", "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "SINGLE", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP155992", null, null, "BA_S16_trimmed.fastq.gz", "fastq", 1302352614.0, 25794210.0, "GSM3317064 r1", "0:50.49 1:0", "A:370194702;C:286011167;G:276758200;T:369297736;N:90809", 50, 0, null, null, 370194702, 286011167, 276758200, 369297736, 90809, "SRX4497223", "SRS3618223", "SRA749599", "GEO", "Duke Molecular Physiology Institute, Duke University Medical Center", 1, 0.89468, null, 0.15745, null, 0.69447, null, 0.47938, null, 50, null, "B", null, "usable mapping rate", "illumina", "hiseq_era", "unknown", "cdna_unspecified", "unknown", "sc", "single_cell_plate", "smartseq", null, "United States", "2018-08-01", "Larval", "Larval", "Endothelium", "Cardiovascular System"]], "columns": ["rowid", "run.accession", "experiment.accession", "sample.accession", "study.accession", "bioproject", "study.title", "study.alias", "study.type", "study.abstract", "study.attributes", "study.PMIDs", "sample.description", "sample.title", "sample.alias", "sample.centername", "sample.attributes", "GEOsample.title", "GEOsample.dataprocessing", "GEOsample.source", "GEOsample.treatmentprotocol", "GEOsample.extractprotocol", "GEOsample.growthprotocol", "GEOsample.characteristics", "GEOsample.accession", "experiment.title", "experiment.alias", "experiment.library_name", "experiment.design_description", "experiment.library_construction_protocol", "experiment.attributes", "experiment.library_strategy", "experiment.library_source", "experiment.library_selection", "experiment.library_layout", "experiment.platform", "experiment.instrument_model", "experiment.spot_descriptor", "experiment.study_ref", "run.title", "run.attributes", "run.filename", "run.semantic_name", "run.total_bases", "run.total_spots", "run.alias", "run.read_lengths", "run.base_counts", "run.r1_length", "run.r2_length", "run.r3_length", "run.r4_length", "run.Acount", "run.Ccount", "run.Gcount", "run.Tcount", "run.Ncount", "run.experiment", "run.pool_member", "submission.accession", "submission.srasource", "submission.bioprojectsource", "seqdetective.n_mates", "seqdetective.mapping_rate.mate1", "seqdetective.mapping_rate.mate2", "seqdetective.nofeature_rate.mate1", "seqdetective.nofeature_rate.mate2", "seqdetective.sparsity.mate1", "seqdetective.sparsity.mate2", "seqdetective.pos_strand_rate.mate1", "seqdetective.pos_strand_rate.mate2", "seqdetective.readlen.mate1", "seqdetective.readlen.mate2", "seqdetective.judgement.mate1", "seqdetective.judgement.mate2", "seqdetective.judgement.reason", "platform_family", "instrument_generation", "read_bias", "selection_class", "prep_kit", "sc_or_bulk", "tech_class", "technology", "tech_variant", "submission.bioprojectsource.country", "earliest_date", "devstage_curation", "devstage_curation_coarse", "tissue_curation", "tissue_curation_coarse"], "primary_keys": ["rowid"], "primary_key_values": ["49042"], "units": {}, "query_ms": 7.850297002732987}