{"database": "metadata", "table": "run_metadata", "rows": [[49013, "SRR7612976", "SRX4477676", "SRS3602906", "SRP155545", "PRJNA483217", "Sequencing of Danio rerio brain for 5 age groups", "GSE117807", "Transcriptome Analysis", "Comparison of temporal small RNA gene expression profiles from Danio rerio brain. The smallRNA seq data comprise 5 age groups at 6  12  24  36 month and 42 month.    Jena Centre for Systems Biology of Ageing   JenAge www.jenage.de Overall design: 25 samples in 5 groups: 6 month 5 samples  12 month 5 samples  24 month 5 samples  36 month 5 samples  42 month 5 samples.", null, null, null, "NH FLI 103 104 ZF brain smallRNAseq", "GSM3309584", null, "source name:brain|strain:AB JxT\\xFC|tissue:brain|age:6 month|Sex:male", "NH FLI 103 104 ZF brain smallRNAseq", "Sequence information was extracted in FastQ format using CASAVA software 1.8.4 data processing step. Reads were trimmed und filtered using trimmomatic 0.36 [parameter: ILLUMINACLIP:smallRNAAdapter:2:6:6 LEADING:15 SLIDINGWINDOW:4:15 MINLEN:20]. Reads were mapped using BWA 0.7.12 r1039 [parameter:  n 0  o 0  e 0  l 8  k 0]. Reads per gene were counted using samtools 1.3.1 9 ge5dfb0a. Genome build: mirBase Release 21 D. rerio sequences only Supplementary files format and content: The Excel file includes raw counts sample counts.xls of all mirBase D. rerio sequences for each sample.", "brain", null, "Extraction was done as described in Baumgart et al. 2012. PMID:22487494 Library preparation was done using Illumina's TruSeq smallRNA Library Preparation Kit following the manufacturer's instruction.", null, "strain:AB JxT\\xFC|tissue:brain|age:6 month|Sex:male", "GSM3309584", "GSM3309584: NH FLI 103 104 ZF brain smallRNAseq; Danio rerio; ncRNA Seq", "GSM3309584", null, "1", "Extraction was done as described in Baumgart et al. 2012. PMID:22487494 Library preparation was done using Illumina's TruSeq smallRNA Library Preparation Kit following the manufacturer's instruction.", "GEO Accession:GSM3309584", "ncRNA-Seq", "TRANSCRIPTOMIC", "size fractionation", "SINGLE", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP155545", null, null, null, null, 1106423150.0, 22128463.0, "GSM3309584 r1", "0:50", "A:234017026;C:276221869;G:334967256;T:261138362;N:78637", 50, null, null, null, 234017026, 276221869, 334967256, 261138362, 78637, "SRX4477676", "SRS3602906", "SRA745937", "GEO", "Leibniz Institute for Age Research - Fritz Lipmann Institute", 1, 0.07804, null, 0.00455, null, 0.99263, null, 0.55094, null, 50, null, "B", null, "usable mapping rate", "illumina", "hiseq_era", "unknown", "size_fractionation", "trueseq", "bulk", "unknown", "unknown", null, "Germany", "2018-07-27", "Adult", "Adult", "Brain", "Nervous System"]], "columns": ["rowid", "run.accession", "experiment.accession", "sample.accession", "study.accession", "bioproject", "study.title", "study.alias", "study.type", "study.abstract", "study.attributes", "study.PMIDs", "sample.description", "sample.title", "sample.alias", "sample.centername", "sample.attributes", "GEOsample.title", "GEOsample.dataprocessing", "GEOsample.source", "GEOsample.treatmentprotocol", "GEOsample.extractprotocol", "GEOsample.growthprotocol", "GEOsample.characteristics", "GEOsample.accession", "experiment.title", "experiment.alias", "experiment.library_name", "experiment.design_description", "experiment.library_construction_protocol", "experiment.attributes", "experiment.library_strategy", "experiment.library_source", "experiment.library_selection", "experiment.library_layout", "experiment.platform", "experiment.instrument_model", "experiment.spot_descriptor", "experiment.study_ref", "run.title", "run.attributes", "run.filename", "run.semantic_name", "run.total_bases", "run.total_spots", "run.alias", "run.read_lengths", "run.base_counts", "run.r1_length", "run.r2_length", "run.r3_length", "run.r4_length", "run.Acount", "run.Ccount", "run.Gcount", "run.Tcount", "run.Ncount", "run.experiment", "run.pool_member", "submission.accession", "submission.srasource", "submission.bioprojectsource", "seqdetective.n_mates", "seqdetective.mapping_rate.mate1", "seqdetective.mapping_rate.mate2", "seqdetective.nofeature_rate.mate1", "seqdetective.nofeature_rate.mate2", "seqdetective.sparsity.mate1", "seqdetective.sparsity.mate2", "seqdetective.pos_strand_rate.mate1", "seqdetective.pos_strand_rate.mate2", "seqdetective.readlen.mate1", "seqdetective.readlen.mate2", "seqdetective.judgement.mate1", "seqdetective.judgement.mate2", "seqdetective.judgement.reason", "platform_family", "instrument_generation", "read_bias", "selection_class", "prep_kit", "sc_or_bulk", "tech_class", "technology", "tech_variant", "submission.bioprojectsource.country", "earliest_date", "devstage_curation", "devstage_curation_coarse", "tissue_curation", "tissue_curation_coarse"], "primary_keys": ["rowid"], "primary_key_values": ["49013"], "units": {}, "query_ms": 7.3681490030139685}